Publication Date:
1992-06-05
Description:
Regulation of interleukin-2 (IL-2) gene expression by the p50 and p65 subunits of the DNA binding protein NF-kappa B was studied in nontransformed CD4+ T lymphocyte clones. A homodimeric complex of the NF-kappa B p50 subunit was found in resting T cells. The amount of p50-p50 complex decreased after full antigenic stimulation, whereas the amount of the NF-kappa B p50-p65 heterodimer was increased. Increased expression of the IL-2 gene and activity of the IL-2 kappa B DNA binding site correlated with a decrease in the p50-p50 complex. Overexpression of p50 repressed IL-2 promoter expression. The switch from p50-p50 to p50-p65 complexes depended on a protein that caused sequestration of the p50-p50 complex in the nucleus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kang, S M -- Tran, A C -- Grilli, M -- Lenardo, M J -- New York, N.Y. -- Science. 1992 Jun 5;256(5062):1452-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Immunology, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1604322" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, CD4/*immunology
;
Base Sequence
;
Binding Sites
;
Cell Line
;
Cell Nucleus/physiology
;
Chloramphenicol O-Acetyltransferase/genetics/metabolism
;
Clone Cells
;
Columbidae
;
DNA/genetics
;
*Gene Expression Regulation
;
Interleukin-2/*genetics
;
Macromolecular Substances
;
Mice
;
Molecular Sequence Data
;
NF-kappa B/*metabolism
;
Oligonucleotide Probes
;
Promoter Regions, Genetic
;
RNA, Messenger/metabolism
;
Recombinant Fusion Proteins/metabolism
;
T-Lymphocyte Subsets/*immunology
;
Transfection
;
Tumor Necrosis Factor-alpha/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
