Publication Date:
1992-02-28
Description:
Severe combined immunodeficient (SCID) mice transplanted with human bone marrow were treated with human mast cell growth factor, a fusion of interleukin-3 and granulocyte-macrophage colony-stimulating factor (PIXY321), or both, starting immediately or 1 month later. Immature human cells repopulated the mouse bone marrow with differentiated human cells of multiple myeloid and lymphoid lineages; inclusion of erythropoietin resulted in human red cells in the peripheral blood. The bone marrow of growth factor-treated mice contained both multipotential and committed myeloid and erythroid progenitors, whereas mice not given growth factors had few human cells and only granulocyte-macrophage progenitors. Thus, this system allows the detection of immature human cells, identification of the growth factors that regulate them, and the establishment of animal models of human hematopoietic diseases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lapidot, T -- Pflumio, F -- Doedens, M -- Murdoch, B -- Williams, D E -- Dick, J E -- New York, N.Y. -- Science. 1992 Feb 28;255(5048):1137-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1372131" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bone Marrow Cells
;
*Bone Marrow Transplantation
;
Cytokines/*pharmacology
;
Erythropoietin/pharmacology
;
Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
;
*Hematopoiesis
;
Hematopoietic Cell Growth Factors/pharmacology
;
*Hematopoietic Stem Cell Transplantation
;
Humans
;
Interleukin-3/pharmacology
;
Mice
;
Mice, SCID
;
Recombinant Fusion Proteins/pharmacology
;
Stem Cell Factor
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
