Publication Date:
1999-06-26
Description:
The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors, but only when GRIP1 or SRC-1a was coexpressed. Thus, CARM1 functions as a secondary coactivator through its association with p160 coactivators. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduced both methyltransferase and coactivator activities. Thus, coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, D -- Ma, H -- Hong, H -- Koh, S S -- Huang, S M -- Schurter, B T -- Aswad, D W -- Stallcup, M R -- AG00093/AG/NIA NIH HHS/ -- DK43093/DK/NIDDK NIH HHS/ -- NS17269/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1999 Jun 25;284(5423):2174-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology HMR 301, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10381882" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Cell Line
;
Histone Acetyltransferases
;
Histones/metabolism
;
Methylation
;
Mice
;
Molecular Sequence Data
;
Mutation
;
Nuclear Receptor Coactivator 1
;
Nuclear Receptor Coactivator 2
;
Nuclear Receptor Coactivator 3
;
Protein-Arginine N-Methyltransferases/chemistry/genetics/*metabolism
;
Receptors, Androgen/metabolism
;
Receptors, Estrogen/metabolism
;
Receptors, Thyroid Hormone/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Signal Transduction
;
Trans-Activators/*metabolism
;
Transcription Factors/metabolism
;
*Transcriptional Activation
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
