Publication Date:
2012-08-04
Description:
Using single-molecule fluorescence resonance energy transfer, we have defined bacterial RNA polymerase (RNAP) clamp conformation at each step in transcription initiation and elongation. We find that the clamp predominantly is open in free RNAP and early intermediates in transcription initiation but closes upon formation of a catalytically competent transcription initiation complex and remains closed during initial transcription and transcription elongation. We show that four RNAP inhibitors interfere with clamp opening. We propose that clamp opening allows DNA to be loaded into and unwound in the RNAP active-center cleft, that DNA loading and unwinding trigger clamp closure, and that clamp closure accounts for the high stability of initiation complexes and the high stability and processivity of elongation complexes.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626110/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626110/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chakraborty, Anirban -- Wang, Dongye -- Ebright, Yon W -- Korlann, You -- Kortkhonjia, Ekaterine -- Kim, Taiho -- Chowdhury, Saikat -- Wigneshweraraj, Sivaramesh -- Irschik, Herbert -- Jansen, Rolf -- Nixon, B Tracy -- Knight, Jennifer -- Weiss, Shimon -- Ebright, Richard H -- AI072766/AI/NIAID NIH HHS/ -- BB/E023703/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- GM037554/GM/NIGMS NIH HHS/ -- GM069709/GM/NIGMS NIH HHS/ -- GM069937/GM/NIGMS NIH HHS/ -- GM41376/GM/NIGMS NIH HHS/ -- R01 AI072766/AI/NIAID NIH HHS/ -- R01 GM041376/GM/NIGMS NIH HHS/ -- R01 GM069709/GM/NIGMS NIH HHS/ -- R37 GM041376/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2012 Aug 3;337(6094):591-5. doi: 10.1126/science.1218716.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Waksman Institute, and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22859489" target="_blank"〉PubMed〈/a〉
Keywords:
DNA Polymerase III/*chemistry/drug effects
;
Fluorescence Resonance Energy Transfer/methods
;
*Gene Expression Regulation, Bacterial
;
Protein Conformation
;
*Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
