Publication Date:
2006-04-15
Description:
The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xie, X Sunney -- Yu, Ji -- Yang, Wei Yuan -- New York, N.Y. -- Science. 2006 Apr 14;312(5771):228-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. xie@chemistry.harvard.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16614211" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cells/*metabolism
;
Fatty Acids/metabolism
;
*Gene Expression
;
*Lipid Metabolism
;
Luminescent Proteins/analysis/metabolism
;
Molecular Motor Proteins/*metabolism
;
*Molecular Probe Techniques
;
Proteins/*metabolism
;
Quantum Dots
;
Spectrum Analysis, Raman
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
