Publication Date:
2001-04-03
Description:
We report the development of a pseudorabies virus that can be used for retrograde tracing from selected neurons. This virus encodes a green fluorescent protein marker and replicates only in neurons that express the Cre recombinase and in neurons in synaptic contact with the originally infected cells. The virus was injected into the arcuate nucleus of mice that express Cre only in those neurons that express neuropeptide Y or the leptin receptor. Sectioning of the brains revealed that these neurons receive inputs from neurons in other regions of the hypothalamus, as well as the amygdala, cortex, and other brain regions. These data suggest that higher cortical centers modulate leptin signaling in the hypothalamus. This method of neural tracing may prove useful in studies of other complex neural circuits.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉DeFalco, J -- Tomishima, M -- Liu, H -- Zhao, C -- Cai, X -- Marth, J D -- Enquist, L -- Friedman, J M -- DK48247/DK/NIDDK NIH HHS/ -- R01133506/PHS HHS/ -- R01DK41096/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2001 Mar 30;291(5513):2608-13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11283374" target="_blank"〉PubMed〈/a〉
Keywords:
Afferent Pathways
;
Animals
;
Arcuate Nucleus of Hypothalamus/cytology/*physiology/virology
;
Brain/cytology/*physiology/virology
;
Brain Mapping
;
Carrier Proteins/genetics/metabolism
;
Chromosomes, Artificial, Bacterial
;
*Eating
;
Gene Expression
;
Green Fluorescent Proteins
;
Herpesvirus 1, Suid/*genetics/physiology
;
Hypothalamus/cytology/*physiology/virology
;
Integrases/genetics/metabolism
;
Luminescent Proteins/genetics/metabolism
;
Mice
;
Mice, Inbred C57BL
;
Mice, Transgenic
;
Neurons/*metabolism/virology
;
Neuropeptide Y/genetics/metabolism
;
*Receptors, Cell Surface
;
Receptors, Leptin
;
Recombinant Fusion Proteins/metabolism
;
Recombination, Genetic
;
*Viral Proteins
;
Virus Replication
;
tau Proteins/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
