ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
High-temperature columns
;
Retention behavior
;
Column equilibration
;
Bilayer formation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Retention volumes (V N) of different kinds of test compound have been measured on silica gel as a function of column temperature (T) in the range 20 to 210°C.n-Heptane and mixtures ofn-heptane and polar modifiers were used as mobile phases. With nC7 and nC7-1,4-dioxane mixtures, there was a good linear relationship between InV N and 1/T for column temperatures above 100 °C. When more polar modifiers such as butanol, ethanol and acetic acid were used, ‘N’-shaped curves were obtained for the plots of InV N against 1/T. Possible explanations of this retention behavior have been proposed on the basis of related studies and common physical chemistry relationships. Column equilibration time after alteration of water content or column temperature is greatly reduced at elevated temperatures. This will benefit the application of LSC on silica gel.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466532
