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Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus brevis (1994)

Louw, M. E. ; Reid, S. J. ; James, D. M. ; [et al.]

Springer

Applied microbiology and biotechnology 42 (1994), S. 78-84

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec− strain as well as in a B. subtilis sacU(HY) strain.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00170228

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