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A method for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum (1974)

Dill, Barbara C. ; Stock, John J.

Springer

Archives of microbiology 96 (1974), S. 281-289

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ISSN: 1432-072X

Keywords: Fungal Nuclei ; Isolation ; Purification ; Characterization ; Dermatophyte ; Microsporum gypseum

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A method is reported for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum. The yield ranged from greater than 90% in crude preparations to less than 20% in highly purified preparations as judged by the recovery of DNA. Nuclei isolated in this manner appeared intact in electron microscopic observations and possessed chemical compositions, i.e. low protein/DNA and RNA/DNA ratios, which indicated a low degree of cytoplasmic contamination.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00590184

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