Abstract
A method is reported for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum. The yield ranged from greater than 90% in crude preparations to less than 20% in highly purified preparations as judged by the recovery of DNA.
Nuclei isolated in this manner appeared intact in electron microscopic observations and possessed chemical compositions, i.e. low protein/DNA and RNA/DNA ratios, which indicated a low degree of cytoplasmic contamination.
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Dill, B.C., Stock, J.J. A method for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum . Arch. Microbiol. 96, 281–289 (1974). https://doi.org/10.1007/BF00590184
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DOI: https://doi.org/10.1007/BF00590184