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Solid phase radioimmunoassay of triiodothyronine (T3) using antibody coupled carboxymethylcellulose powder (1987)

Pillai, M. R. A. ; Narayanan, Jyotsna ; Gupte, J. H. ; [et al.]

Springer

Journal of radioanalytical and nuclear chemistry 116 (1987), S. 317-323

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ISSN: 1588-2780

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering

Notes: Abstract The paper describes a solid-phase radioimmunoassay for triiodothyronine (T3) using antibody coupled to carboxymethylcellulose powder. The free carboxylic acid groups of cellulose are covalently coupled to the amino groups of the antibody using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride. Immobilized antibody thus prepared was used in a solid-phase radioimmunoassay of T3. The assay has a sensitivity of 0.18 μg/l, and a range of 0.18–4 μg/l. Satisfactory correlation was obtained when this assay was compared with a T3 assay based on dextran coated charcoal separation system (Y=0.95X+0.15 μg/L; r=0.98).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02035775

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