Publication Date:
2021-05-19
Description:
Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus frisii kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500gl tubes and amplified using PCR method with a pair of primers with cytochrome b gene sequence of Kora volba.
For RFLP analysis, PCR products of cytochrome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I, Hhal, Hinc II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinfl, flha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
Description:
Published
Keywords:
Genetic
;
Species
;
Cyprinidae
;
PCR-RFLP
;
Molecular Marker
;
Rutilus frisii kutum
;
Specimens
;
Barbus capito
;
Bream Abramis brama
;
Aspius aspius
;
Rutilus rutilus
;
DNA
;
Enzymes
Repository Name:
AquaDocs
Type:
Journal Contribution
,
Refereed
Format:
pp.49-58
