Call number:
AWI Bio-24-95664
Description / Table of Contents:
This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small to jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture, molecular techniques, protocols for isolate, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.
Type of Medium:
Monograph available for loan
Pages:
XVI, 431 Seiten
,
Illustrationen
ISBN:
978-1-0716-3548-3
,
9781071635483
ISSN:
1064-3745
,
1940-6029
Series Statement:
Methods in Molecular Biology 2738
DOI:
10.1007/978-1-0716-3549-0
Language:
English
Note:
Contents
Preface
Contributors
PART I AN OVERVIEW OF THE DIVERSITY OF BACTERIOPHAGES
1 Structural and Genomic Diversity of Bacteriophages / Bert Ely, Jacob Lenski, and Tannaz Mohammadi
2 The Diversity of Bacteriophages in the Human Gut / Amanda Carroll-Portillo, Derek M. Lin, and Henry C. Lin
3 Breaking the Ice: A Review of Phages in Polar Ecosystems / Mara Elena Heinrichs, Gonçalo J. Piedade, Ovidiu Popa, Pacifica Sommers, Gareth Trubl, Julia Weissenbach, and Janina Rahlff
4 The Diversity of Bacteriophages in Hot Springs / Timothy J. Marks and Isabella R. Rowland
PART II ISOLATION OF BACTERIOPHAGES
5 Isolation of Bacteriophages from Soil Samples in a Poorly Equipped Field Laboratory in Kruger National Park / Ayesha Hassim and Kgaugelo Edward Lekota
6 Purification and Up-Concentration of Bacteriophages and Viruses from Fecal Samples / Frej Larsen, Rasmus Riemer Jakobsen, Xiaotian Mao,
Josue Castro-Mejia, Ling Deng, and Dennis S. Nielsen
7 Isolation of Enterococcus Bacteriophages from Municipal Wastewater Samples Using an Enrichment Step / Cory Schwarz and Jacques Mathieu
8 Phage DNA Extraction, Genome Assembly, and Genome Closure / Justin Boeckman, Mei Liu, Jolene Ramsey, and Jason Gill
PART III ENUMERATION OF BACTERIOPHAGES
9 Enumeration of Bacteriophages by Plaque Assay / Diana Elizabeth Waturangi
10 Detection and Quantification of Bacteriophages in Wastewater Samples by Culture and Molecular Methods/ Laura Sala-Comorera, Maite Muniesa, and Lorena Rodríguez-Rubio
11 Flow Virometry: A Fluorescence-Based Approach to Enumerate Bacteriophages in Liquid Samples / Elena A. Dlusskaya and Rafik Dey
12 A Metagenomics Approach to Enumerate Bacteriophages in a Food Niche / Kelsey White, Giovanni Eraclio, Gabriele Andrea Lugli, Marco Ventura, Jennifer Mahony, Fabio Dal Bello, and Douwe van Sinderen
PART IV CHARACTERIZATION OF BACTERIOPHAGES
13 Bioinformatic Analysis of Staphylococcus Phages: A Key Step for Safe Cocktail Development / Soledad Telma Carrasco and He´ctor Ricardo Morbidoni
14 Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus / Chun-Feng David Hou, Fenglin Li, Stephano Iglesias, and Gino Cingolani
15 Bacteriophage–Host Interactions and Coevolution / Diana M. Álvarez-Espejo, Dácil Rivera, and Andrea I. Moreno-Switt
16 Unraveling Physical Interactions of Clostridioides difficile with Phage and Phage-Derived Proteins Using In Vitro and Whole-Cell Assays / Wichuda Phothichaisri, Tanaporn Phetruen, Surang Chankhamhaengdecha, Tavan Janvilisri, Puey Ounjai, Robert P. Fagan, and Sittinan Chanarat
17 Phage Transduction of Staphylococcus aureus / Melissa-Jane Chu Yuan Kee and John Chen
PART V APPLICATION OF BACTERIOPHAGES AND BACTERIOPHAGE-DERIVED COMPONENTS
18 The Next Generation of Drug Delivery: Harnessing the Power of Bacteriophages / Alaa A. A. Aljabali, Mohammad B. M. Aljbaly, Mohammad A. Obeid, Seyed Hossein Shahcheraghi, and Murtaza M. Tambuwala
19 Construction of Nonnatural Cysteine-Cross-Linked Phage Libraries / Brittney Chau, Kristi Liivak, and Jianmin Gao
20 Application of Deep Sequencing in Phage Display / Vincent Van Deuren, Sander Plessers, Rob Lavigne, and Johan Robben
21 The Application of Bacteriophage and Photoacoustic Flow Cytometry in Bacterial Identification / Robert H. Edgar, Anie-Pier Samson, and John A. Viator
22 Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy / Katarzyna Kosznik-Kwaśnicka, Gracja Topka, Jagoda Mantej, Łukasz Grabowski, Agnieszka Necel, Grzegorz Węgrzyn, and Alicja Węgrzyn
23 Overcoming Bacteriophage Resistance in Phage Therapy / Elina Laanto
24 Bacteriophage Virus-Like Particles: Platforms for Vaccine Design / Ebenezer Tumban
Index
Location:
AWI Reading room
Branch Library:
AWI Library
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