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  • Life Sciences (General)  (2,833)
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  • 1
    Publication Date: 2021-01-30
    Description: The first meeting of the South West Indian Ocean EAF Regional Task Group (RTG) was held in Mombasa, Kenya, from 27 to 30 January 2009, together with an ecological risk assessment methodology workshop. It was attended by 20 partic ipants from the South West Indian Ocean (SWIO) countries, the South West Indian Ocean Fisheries Project (SWIOFP), the Agulhas and Somali Currents Large Marine Ecosystems (ASCLME) project, the Scientific Committee of the South West Indian Ocean Fisheries Commission, the United Nations Environment Programme (UNEP)/Nairobi Convention Implementation Unit, the African Union Commission and FAO. The RTG is an implementation structure under the EAF-Nansen project GCP/INT/003/NOR and serves as the forum for training in ecological risk assessment that is the methodology used for the identification and prioritization of issues requiring management attention. The main objectives of the meeting and workshop we re to discuss and facilitate key processes and activities for the implementation of the ecosystem approach to fisheries management in the South West Indian Ocean region including the modalities for the formation and functioning of the RTG and National Task Groups (NTGs). It was explained that, to be able to achieve the objectives of implementing an ecosystem approach to fisheries at the national level, certain key structures have to be in place including the NTG with representatives of key stakeholders in a given fishery and that would take the lead in the process. An overview of the key concepts and process of the ecological risk assessment methodology were clarified. Participants were also introduced to the preparation of EAF baseline reports to be used as initial input for the work on ecosystems approach to fisheries. It was explained that the preparation of the report is to be led by national and regional experts and overseen by the NTG. For the exercises the participants worked in three subgroups formed during the meeting with each group selecting a chairman who moderate d the discussions and a rapporteur. The participants expressed satisfaction with the development of a communication strategy for the project and especially with the participatory approach used.
    Description: FAO, NORAD, Institute of Marine Research
    Description: Published
    Description: National task groups
    Description: Regional task groups
    Description: Ecosystem Approach to Fisheries
    Keywords: Ecosystems ; Fisheries ; Ecosystems ; Fishery resources ; Fisheries management
    Repository Name: AquaDocs
    Type: Report , Non-Refereed
    Format: 36
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  • 2
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    Nigerian Institute for Oceanography and Marine Research | Lagos, Nigeria
    Publication Date: 2021-01-30
    Description: Published
    Description: Fisheries newsletter
    Keywords: Fisheries ; Marine sciences ; Fisheries literature
    Repository Name: AquaDocs
    Type: Report , Non-Refereed
    Format: 30pp.
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  • 3
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    Nigerian Institute for Oceanography and Marine Research | Lagos, Nigeria
    Publication Date: 2021-01-30
    Description: Published
    Description: Fisheries newsletter
    Keywords: Fisheries ; Marine sciences ; Fisheries literature
    Repository Name: AquaDocs
    Type: Report , Non-Refereed
    Format: 34pp.
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  • 4
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    Nigerian Institute for Oceanography and Marine Research | Lagos, Nigeria,
    Publication Date: 2021-01-30
    Description: Published
    Description: Fisheries newsletter
    Keywords: Fisheries ; Fisheries literature
    Repository Name: AquaDocs
    Type: Report , Non-Refereed
    Format: 11pp.
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  • 5
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    Nigerian Institute for Oceanography and Marine Research, | Lagos, Nigeria,
    Publication Date: 2021-01-30
    Description: Published
    Description: Fisheries newsletter
    Keywords: Fisheries ; Fisheries literature
    Repository Name: AquaDocs
    Type: Report , Non-Refereed
    Format: 8pp.
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  • 6
    Publication Date: 2019-07-27
    Description: In May 2007, what was then the Space Life Sciences Directorate published the 2007 Space Life Sciences Strategy for Human Space Exploration, which resulted in the development and implementation of new business models and significant advances in external collaboration over the next five years. The strategy was updated on the basis of these accomplishments and reissued as the NASA Human Health and Performance Strategy in 2012, and continues to drive new approaches to innovation for the directorate. This short paper describes the open innovation successes and collaborative projects developed over this timeframe, including the efforts of the NASA Human Health and Performance Center (NHHPC), which was established to advance human health and performance innovations for spaceflight and societal benefit via collaboration in new markets.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30438
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  • 7
    Publication Date: 2019-08-03
    Description: The case for life on Mars grows stronger. Investigations at Gale Crater by Curiosity have revealed fine-grained sedimentary rocks inferred to represent an ancient lake environment suited to support life. In addition, Curiosity tentatively found a heterogeneous distribution of organic carbon within these sediments, consistent with the detection of native organic C in Mars meteorites. Furthermore, modern potentially habitable environments have been recognized on Mars including the N. Polar region visited by Phoenix, gully features suggesting modern water flows, and RSLs that occur seasonally suggest liquid processes. The time is ripe for missions to Mars incorporating a search for biochemical evidence of life.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN15436 , International Conference on Mars; Jul 14, 2014 - Jul 18, 2014; Pasadena, CA; United States
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  • 8
    Publication Date: 2019-07-19
    Description: We have become impatient waiting for a web page to load, but the first member of our species evolved about 150,000 years ago - a geological instant as brief and as transitory as a text message. The shortest generation time of a bacterium is a sprint at under ten minutes, whereas a 200-year old whale, turtle or tree is not unknown. Life is a phenomenon that integrates processes ranging from the near instantaneous reactions of photosynthesis to the more stately pace of evolution. Here I will elucidate these processes with radically different time scales that go to creating and maintaining the diversity of life on earth, the clocks that nature uses to time them, and how modern biology is being used to alter the natural time scales.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN11984 , Spring 2014 Biology Seminar Series; Mar 20, 2014; Williamsburg, VA; United States
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  • 9
    Publication Date: 2019-07-19
    Description: Starting in 2015, the NASA Bioculture System will be available to the science community to conduct cell biology and microbiology experiments on ISS. The Bioculture System carries ten environmentally independent Cassettes, which house the experiments. The closed loop fluids flow path subsystem in each Cassette provides a perfusion-based method for maintain specimen cultures in a shear-free environment by using a biochamber based on porous hollow fiber bioreactor technology. Each Cassette contains an incubator and separate insulated refrigerator compartment for storage of media, samples, nutrients and additives. The hardware is capable of fully automated or manual specimen culturing and processing, including in-flight experiment initiation, sampling and fixation, up to BSL-2 specimen culturing, and the ability to up to 10 independent cultures in parallel for statistical analysis. The incubation and culturing of specimens in the Bioculture System is a departure from standard laboratory culturing methods. Therefore, it is critical that the PI has an understanding the pre-flight test required for successfully using the Bioculture System to conduct an on-orbit experiment. Overall, the PI will conduct a series of ground tests to define flight experiment and on-orbit implementation requirements, verify biocompatibility, and determine base bioreactor conditions. The ground test processes for the utilization of the Bioculture System, from experiment selection to flight, will be reviewed. Also, pre-flight test schedules and use of COTS ground test equipment (CellMax and FiberCell systems) and the Bioculture System will be discussed.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN16080 , Annual Meeting of the American Society for Gravitational And Space Research; Oct 22, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 10
    Publication Date: 2019-07-19
    Description: The European Modular Cultivation System, EMCS, was developed by ESA for plant experiments. We performed ground testing to determine whether ARC EMCS seed cassettes could be adapted for use with tardigrades for future spaceflight experiments. Tardigrades (water bears) are small invertebrates that enter the tun state in response to desiccation or other environmental stresses. Tardigrade tuns have suspended metabolism and have been shown to be survive exposure to space vacuum, high pressure, temperature and other stresses. For spaceflight experiments using the EMCS, the organisms ideally must be able to survive desiccation and storage in the cassette at ambient temperature for several weeks prior to the initiation of the experiment by the infusion of water to the cassette during spaceflight. The ability of tardigrades to survive extremes by entering the tun state make them ideal candidates for growth experiments in the EMCS cassettes. The growth substratum in the cassettes is a gridded polyether sulfone (PES) membrane. A blotter beneath the PES membrane contains dried growth medium. The goals of our study were to (1) determine whether tardigrades survive and reproduce on PES membranes, (2) develop a consistent method for dehydration of the tardigrades with high recovery rates upon rehydration, (3) to determine an appropriate food source for the tardigrades that can also be dehydrated/rehydrated and (4) successful mock rehydration experiment in cassettes with appropriate food source. We present results that show successful multigenerational growth of tardigrades on PES membranes with a variety of wet food sources. We have successfully performed a mock rehydration with tardigrades and at least one candidate food, protonema of the moss Polytrichum, that supports multigenerational growth and whose spores germinate quickly enough to match tardigrade feeding patterns post rehydration. Our results indicate that experiments on the ISS using the tardigrade, Hypsibius dujardini and other similar species could successfully be performed in the flight verified hardware of the EMCS seed cassettes.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN16154 , Annual Meeting of the American Society for Gravitational and Space Research; Oct 23, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 11
    Publication Date: 2019-07-19
    Description: The Moon's surface is covered by a layer of fine, potential reactive dust. Lunar dust contain about 12% of very fine respirable dust (less than 3 micrometers). The habitable area of any lunar landing vehicle and outpost would inevitably be contaminated with lunar dust that could pose a health risk. The purpose of the study is to evaluate the toxicity of Apollo moon dust in rodents to assess the health risk of dust exposures to humans. One of the particular interests in the study is to evaluate dustinduced changes of the expression of fibrosisrelated genes, and to identify specific signaling pathways involved in lunar dustinduced toxicity. F344 rats were exposed for 4 weeks (6h/d; 5d/wk) in noseonly inhalation chambers to concentrations of 0 (control air), 2.1, 6.8, 21, and 61 milligrams per cubic meters of lunar dust. Five rats per group were euthanized at 1 day, 1 week, 1 month, and 3 months after the last inhalation exposure. The bronchoalveolar lavage fluid (BALF) was collected by lavaging with phosphatebuffered saline (PBS). A zymosaninduced luminolbased chemiluminescence assay was used to assess the activity of BAL cells. The lavaged lung tissue was snap frozen in LN2 and total RNA was isolated using the Qigen RNeasy kit. The expression of 84 fibrosisrelated genes were analyzed using the RT2 Profiler PCR Array technique. The expression of 18 genes of interest were further measured using realtime PCR technique in all the samples. 10 out of 18 genes of interest showed persistently significant expression changes in the local lung tissue exposed to lunar dust, indicating a prolonged proinflammatory response. The expressions of several of these genes were dose and timedependent and were significantly correlated with other pathological parameters. The potential signaling pathways and upstream regulators were further analyzed using IPA pathway analysis tool based on the gene expression data. The data presented in this study, for the first time, explore the molecular mechanisms of lunar dust induced toxicity, contributing not only the risk assessment for future space exploration, but also understandings of the dustinduced toxicity in humans on earth.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30593 , COSPAR Meeting; Aug 02, 2014 - Aug 10, 2014; Moscow; Russia
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  • 12
    Publication Date: 2019-07-20
    Description: Rodent research in space is needed to advance our understanding of the health risks,consequences and possible countermeasures to protect crew during future, long duration missions. TheAnimal Enclosure Module (AEM) was designed originally to support habitation of rats and mice onrelatively short duration, Shuttle missions (〈19 days). The AEM was flown previously on 27 SpaceShuttle missions, and recently was modified extensively to support future long duration space biology andbiomedical research on the International Space Station (ISS). In consultation with a Science WorkingGroup comprised of veterinarians and investigators experienced in rodent spaceflight experimentation inspace, the Rodent Habitat project team at Ames Research Center modified existing hardware, developednew hardware, operations, and science activities, and performed a series of ground-based operational andscience habitat verification tests in preparation for the first validation flight.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN12037 , NASA Human Research Program Investigatorsý Workshop (HRP 2014); Feb 12, 2014 - Feb 13, 2014; Galveston, TX; United States
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  • 13
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    In:  CASI
    Publication Date: 2019-07-13
    Description: Presentation to POIWG meeting at MSFC to discuss planned operations for upcoming FFL-01 mission on SpaceX-5. Will show hardware suite used, on-orbit operations, training strategy, and data handling architecture.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN13057 , Payload Operations and Integration Working Group (POIWG); Jan 28, 2014; Huntsville, AL; United States
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  • 14
    Publication Date: 2019-07-13
    Description: Understanding the genetic, physiological, and behavioral effects of spaceflight on living organisms and elucidating the molecular mechanisms that underlie these effects are high priorities for NASA. Certain organisms, known as model organisms, are widely studied to help researchers better understand how all biological systems function. Small model organisms such as nem-atodes, slime mold, bacteria, green algae, yeast, and moss can be used to study the effects of micro- and reduced gravity at both the cellular and systems level over multiple generations. Many model organisms have sequenced genomes and published data sets on their transcriptomes and proteomes that enable scientific investigations of the molecular mechanisms underlying the adaptations of these organisms to space flight.
    Keywords: Life Sciences (General)
    Type: NASA-FS-2014-10-01-ARC , ARC-E-DAA-TN18374 , American Society for Gravitational and Space Research (ASGSR 2014) Meeting; Oct 22, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 15
    Publication Date: 2019-07-13
    Description: Rodent Research on the International Space Station (ISS) is one of the highest priority science activities being supported by NASA and is planned for up to two flights per year. The first Rodent Research flight, Rodent Research-1 (RR-1) validates the hardware and basic science operations (dissections and tissue preservation). Subsequent flights will add new capabilities to support rodent research on the ISS. RR-1 will validate the following capabilities: animal husbandry for up to 30 days, video downlink to support animal health checks and scientific analysis, on-orbit dissections, sample preservation in RNA. Later and formalin, sample transfer from formalin to ethanol (hindlimbs), rapid cool-down and subsequent freezing at -80 of tissues and carcasses, sample return and recovery. RR-2, scheduled for SpX-6 (Winter 20142015) will add the following capabilities: animal husbandry for up to 60 days, RFID chip reader for individual animal identification, water refill and food replenishment, anesthesia and recovery, bone densitometry, blood collection (via cardiac puncture), blood separation via centrifugation, soft tissue fixation in formalin with transfer to ethanol, and delivery of injectable drugs that require frozen storage prior to use. Additional capabilities are also planned for future flights and these include but are not limited to male mice, live animal return, and the development of experiment unique equipment to support science requirements for principal investigators that are selected for flight. In addition to the hardware capabilities to support rodent research the Crew Office has implemented a training program in generic rodent skills for all USOS crew members during their pre-assignment training rotation. This class includes training in general animal handling, euthanasia, injections, and dissections. The dissection portion of this training focuses on the dissection of the spleen, liver, kidney with adrenals, brain, eyes, and hindlimbs. By achieving and maintaining proficiency in these basic skills as part of the nominal astronaut training curriculum this allows the rodent research program to focus the mission specific crew training on scientific requirements of research and operations flow.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN18472 , Annual American Society for Gravitational and Space Research; Oct 23, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 16
    Publication Date: 2019-07-13
    Description: We have shown using ESA's Biopan facility flown in Earth orbit that when exposed to the space environment for 2 weeks the survival rate of Synechococcus (Nageli), a halophilic cyanobacterium isolated from the evaporitic gypsum-halite crusts that form along the marine intertidal, and Halorubrum chaoviator a member of the Halobacteriaceae isolated from an evaporitic NaCl crystal obtained from a salt evaporation pond, were higher than all other test organisms except Bacillus spores. These results led to the EXPOSE-R mission to extend and refine these experiments as part of the experimental package for the external platform space exposure facility on the ISS. The experiment was flown in February 2009 and the organisms were exposed to low-Earth orbit for nearly 2 years. Samples were either exposed to solar ultraviolet (UV)-radiation (lambda is greater than 110 nm or lambda is greater than 200 nm, cosmic radiation (dosage range 225-320 mGy), or kept in darkness shielded from solar UV-radiation. Half of each of the UV-radiation exposed samples and dark samples were exposed to space vacuum and half kept at 105 pascals in argon. Duplicate samples were kept in the laboratory to serve as unexposed controls. Ground simulation control experiments were also performed. After retrieval, organism viability was tested using Molecular Probes Live-Dead Bac-Lite stain and by their reproduction capability. Samples kept in the dark, but exposed to space vacuum had a 90 +/- 5% survival rate compared to the ground controls. Samples exposed to full UV-radiation for over a year were bleached and although results from Molecular Probes Live-Dead stain suggested approximately 10% survival, the data indicate that no survival was detected using cell growth and division using the most probable number method. Those samples exposed to attenuated UV-radiation exhibited limited survival. Results from of this study are relevant to understanding adaptation and evolution of life, the future of life beyond earth, the potential for interplanetary transfer of viable microbes via meteorites and dust particles as well as spacecraft, and the physiology of halophiles.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN23546 , International Journal of Astrobiology; 14; 1; 123-128
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  • 17
    Publication Date: 2019-07-12
    Description: The goal of this project is to select and advance methods to enable real-time sampling, microbiological analysis, and sanitation of crops grown on the International Space Station (ISS). These methods would validate the microbiological quality of crops grown for consumption to ensure safe and palatable fresh foods. This would be achieved through the development / advancement of microbiological sample collection, rapid pathogen detection and effective sanitation methods that are compatible with a microgravity environment.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN14972
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  • 18
    Publication Date: 2019-07-12
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN13837
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  • 19
    Publication Date: 2019-07-12
    Description: During hindlimb unloading (HU) dramatic fluid shifts occur within minutes of the suspension, leading to a less precise matching of blood flow to O2 demands of skeletal muscle. Vascular resistance directs blood away from certain muscles, such as the soleus (SOL). The muscle volume gradually reduces in these muscles so that eventually the relative blood flow returns to normal. It is generally believed that muscle volume change is not due to O2 depletion, but a consequence of disuse. However, the volume of the unloaded rat muscle declines over the course of weeks, whereas the redistribution of blood flow occurs immediately. Using a Krogh Cylinder Model, the distribution of O2 was predicted in two skeletal muscles: SOL and gastrocnemius (GAS). Effects of the muscle blood flow, volume, capillary density, and O2 uptake, are included to calculate the pO2 at rest and after 10 min and 15 days of unloading. The model predicts that 32 percent of the SOL muscle tissue has a pO2 1.25 mm Hg within 10 min, whereas the GAS maintains normal O2 levels, and that equilibrium is reached only as the SOL muscle cells degenerate. The results provide evidence that there is an inadequate O2 supply to the mitochondria in the SOL muscle after 10 min HU.
    Keywords: Life Sciences (General)
    Type: NASA/TM-2014-216631 , E-18834 , GRC-E-DAA-TN12351
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  • 20
    Publication Date: 2019-07-19
    Description: Life has had a profound impact on the geological history of our planet, which in turn has had a profound impact back on the evolution of life. Life has been able to adapt and spread into every planetary nook and cranny. At this point in history, life is becoming able to engineer itself, with extreme consequences we are only dimly able to foresee. One probable outcome will be the facilitation of the expansion of the range of life to beyond our planetary cradle, an evolutionary step as profound as the ancient transition from sea to land. Current efforts at NASA and aboard the International Space Station will be discussed in this context.
    Keywords: Life Sciences (General)
    Type: JSC-CN-32196
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  • 21
    Publication Date: 2019-07-19
    Description: Pollen from members of the Cupressaceae are major aeroallergens in many parts of the world. In the south central and southwest United States, Juniperus pollen is the most important member of this family with J. ashei (JA) responsible for severe winter allergy symptoms in Texas and Oklahoma. In New Mexico, pollen from J. monosperma (JM) and other Juniperus species are important contributors to spring allergies, while J. pinchotii (JP) pollinates in the fall affecting sensitive individuals in west Texas, southwest Oklahoma and eastern New Mexico. Throughout this region, JA, JM, and JP occur in dense woodland populations. Generally monitoring for airborne allergens is conducted in urban areas, although the source for tree pollen may be forested areas distant from the sampling sites. Improved pollen forecasts require a better understanding of pollen production at the source. The current study was undertaken to examine the aerobiology of several Juniperus species at their source areas for the development of new pollen forecasting initiatives.
    Keywords: Life Sciences (General)
    Type: M14-3771 , International Congress of Biometeorology; Sep 28, 2014 - Oct 02, 2014; Cleveland, OH; United States
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  • 22
    Publication Date: 2019-07-19
    Description: The aim of our ground testing was to demonstrate the capability of safely putting specific model organisms into dehydrated stasis, and to later rehydrate and successfully grow them inside flight proven ARC EMCS seedling cassettes. The ARC EMCS seedling cassettes were originally developed to support seedling growth during space flight. The seeds are attached to a solid substrate, launched dry, and then rehydrated in a small volume of media on orbit to initiate the experiment. We hypothesized that the same seedling cassettes should be capable of acting as culture chambers for a wide range of organisms with minimal or no modification. The ability to safely preserve live organisms in a dehydrated state allows for on orbit experiments to be conducted at the best time for crew operations and more importantly provides a tightly controlled physiologically relevant growth experiment with specific environmental parameters. Thus, we performed a series of ground tests that involved growing the organisms, preparing them for dehydration on gridded Polyether Sulfone (PES) membranes, dry storage at ambient temperatures for varying periods of time, followed by rehydration. Inside the culture cassettes, the PES membranes were mounted above blotters containing dehydrated growth media. These were mounted on stainless steel bases and sealed with plastic covers that have permeable membrane covered ports for gas exchange. The results showed we were able to demonstrate acceptable normal growth of C.elegans (nematodes), E.coli (bacteria), S.cerevisiae (yeast), Polytrichum (moss) spores and protonemata, C.thalictroides (fern), D.discoideum (amoeba), and H.dujardini (tardigrades). All organisms showed acceptable growth and rehydration in both petri dishes and culture cassettes initially, and after various time lengths of dehydration. At the end of on orbit ISS European Modular Cultivation System experiments the cassettes could be frozen at ultra-low temperatures, refrigerated, or chemically preserved before being returned to Earth for analyses. Our results suggest that with protocol modifications and future verification testing we can utilize the versatile EMCS to conduct tightly controlled experiments inside our culture cassettes for a wide variety of organisms. These physiological experiments would be designed to answer questions at the molecular level about the specific stress responses of space flight.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN15861 , Annual American Society for Gravitational and Space Research; Oct 23, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 23
    Publication Date: 2019-07-19
    Description: The Veggie hardware validation test, VEG-01, was conducted on the International Space Station during Expeditions 39 and 40 from May through June of 2014. The Veggie hardware and the VEG-01 experiment payload were launched to station aboard the SpaceX-3 resupply mission in April, 2014. Veggie was installed in an Expedite-the-Processing-of-Experiments-to-Space-Station (ExPRESS) rack in the Columbus module, and the VEG-01 validation test was initiated. Veggie installation was successful, and power was supplied to the unit. The hardware was programmed and the root mat reservoir and plant pillows were installed without issue. As expected, a small amount of growth media was observed in the sealed bags which enclosed the plant pillows when they were destowed. Astronaut Steve Swanson used the wet/dry vacuum to clean up the escaped particles. Water insertion or priming the first plant pillow was unsuccessful as an issue prevented water movement through the quick disconnect. All subsequent pillows were successfully primed, and the initial pillow was replaced with a backup pillow and successfully primed. Six pillows were primed, but only five pillows had plants which germinated. After about a week and a half it was observed that plants were not growing well and that pillow wicks were dry. This indicated that the reservoir was not supplying sufficient water to the pillows via wicking, and so the team reverted to an operational fix which added water directly to the plant pillows. Direct watering of the pillows led to a recovery in several of the stressed plants; a couple of which did not recover. An important lesson learned involved Veggie's bellows. The bellows tended to float and interfere with operations when opened, so Steve secured them to the baseplate during plant tending operations. Due to the perceived intensity of the LED lights, the crew found it challenging to both work under the lights and read crew procedures on their computer. Although the lights are not a safety hazard, for visual comfort crewmembers were advised to wear sunglasses when working with the plants and then they can lift glasses to read procedures. Steve Swanson had already trail-blazed this procedure when he initiated VEG-01. The temperature and humidity data logger was relocated mid-experiment to provide measurements on both sides of the unit. Images of the plants were downlinked weekly, and videos of installation and harvest were recorded. This imaging frequency was not sufficient to monitor and respond to changes in plant growth. Plants, samples, and data loggers will be returned on SpaceX-4, scheduled to return the fall of 2014. Lessons learned will be translated into hardware and operational modifications for future Veggie payloads.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN15514 , American Society for Gravitational and Space Research 2014; Oct 22, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 24
    Publication Date: 2019-07-19
    Description: From its inception in 2000, one of the primary tasks of the Biomedical Data Reduction Analysis (BDRA) group has been translation of large amounts of data into information that is relevant to the audience receiving it. BDRA helps translate data into an integrated model that supports both operational and research activities. This data integrated model and subsequent visual data presentations have contributed to BDRA's success in delivering the message (i.e., the story) that its customers have needed to communicate. This success has led to additional collaborations among groups that had previously not felt they had much in common until they worked together to develop solutions in an integrated fashion. As more emphasis is placed on working with "big data" and on showing how NASA's efforts contribute to the greater good of the American people and of the world, it becomes imperative to visualize the story of our data to communicate the greater message we need to share. METHODS To create and expand its data integrated model, BDRA has incorporated data from many different collaborating partner labs and other sources. Data are compiled from the repositories of the Lifetime Surveillance of Astronaut Health and the Life Sciences Data Archive, and from the individual laboratories at Johnson Space Center that support collection of data from medical testing, environmental monitoring, and countermeasures, as designated in the Medical Requirements Integration Documents. Ongoing communication with the participating collaborators is maintained to ensure that the message and story of the data are retained as data are translated into information and visual data presentations are delivered in different venues and to different audiences. RESULTS We will describe the importance of storytelling through an integrated model and of subsequent data visualizations in today's scientific presentations and discuss the collaborative methods used. We will illustrate the discussion with examples of graphs from BDRA's past work supporting operations and/or research efforts.
    Keywords: Life Sciences (General)
    Type: JSC-CN-32245 , 2015 NASA Human Research Program Investigators'' Workshop (HRP IWS 2015); Jan 13, 2015 - Jan 15, 2015; Galveston, TX; United States
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  • 25
    Publication Date: 2019-07-19
    Description: The Moon's surface is covered by a layer of fine, potential reactive dust. Lunar dust contain about 12% respirable very fine dust (less than 3 micrometers). The habitable area of any lunar landing vehicle and outpost would inevitably be contaminated with lunar dust that could pose a health risk. The purpose of the study is to analyze the dynamics of global gene expression changes in lung tissues of rats exposed to lunar dust particles. F344 rats were exposed for 4 weeks (6h/d; 5d/wk) in noseonly inhalation chambers to concentrations of 0 (control air), 2.1, 6.8, 21, and 61 mg/m3 of lunar dust. Animals were euthanized at 1 day and 13 weeks after the last inhalation exposure. After being lavaged, lung tissue from each animal was collected and total RNA was isolated. Four samples of each dose group were analyzed using Agilent Rat GE v3 microarray to profile global gene expression of 44K transcripts. After background subtraction, normalization, and log transformation, t tests were used to compare the mean expression levels of each exposed group to the control group. Correction for multiple testing was made using the method of Benjamini, Krieger, and Yekuteli (1) to control the false discovery rate. Genes with significant changes of at least 1.75 fold were identified as genes of interest. Both low and high doses of lunar dust caused dramatic, dosedependent global gene expression changes in the lung tissues. However, the responses of lung tissue to low dose lunar dust are distinguished from those of high doses, especially those associated with 61mg/m3 dust exposure. The data were further integrated into the Ingenuity system to analyze the gene ontology (GO), pathway distribution and putative upstream regulators and gene targets. Multiple pathways, functions, and upstream regulators have been identified in response to lunar dust induced damage in the lung tissue.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30592 , COSPAR Meeting; Aug 02, 2014 - Aug 10, 2014; Moscow; Russia
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  • 26
    Publication Date: 2019-07-19
    Description: Chromosomal translocations and inversions are considered stable, and cells containing these types of chromosome aberrations can survive multiple cell divisions. An efficient method to detect an inversion is multi-color banding fluorescent in situ hybridization (mBAND) which allows identification of both inter- and intrachromosome aberrations simultaneously. Post irradiation, chromosome aberrations may also arise after multiple cell divisions as a result of genomic instability. To investigate the stable or late-arising chromosome aberrations induced after radiation exposure, we exposed human lymphocytes to gamma rays and Fe ions ex vivo, and cultured the cells for multiple generations. Chromosome aberrations were analyzed in cells collected at first mitosis and at several time intervals during the culture period post irradiation. With gamma irradiation, about half of the damages observed at first mitosis remained after 7 day- and 14 day- culture, suggesting the transmissibility of damages to the surviving progeny. Detailed analysis of chromosome break ends participating in exchanges revealed a greater fraction of break ends involved in intrachromosome aberrations in the 7- and 14-day samples in comparison to the fraction at first mitosis. In particular, simple inversions were found at 7 and 14 days, but not at the first mitosis, suggesting that some of the aberrations might be formed days post irradiation. In contrast, at the doses that produced similar frequencies of gamma-induced chromosome aberrations as observed at first mitosis, a significantly lower yield of aberrations remained at the same population doublings after Fe ion exposure. At these equitoxic doses, more complex type aberrations were observed for Fe ions, indicating that Fe ion-induced initial chromosome damages are more severe and may lead to cell death. Comparison between low and high doses of Fe ion irradiation in the induction of late damages will also be discussed.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30645 , COSPAR Scientific Assembly; Aug 02, 2014 - Aug 10, 2014; Moscow; Russia
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  • 27
    facet.materialart.
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    In:  CASI
    Publication Date: 2019-07-19
    Description: RNA world theories figure prominently in many scenarios for the origin and early evolution of life. These theories posit that RNA molecules played a much larger role in ancient biology than they do now, acting both as the dominant biocatalysts and as the repository of genetic information. Many features of modern RNA biology are potential examples of molecular fossils from an RNA world, such as the pervasive involvement of nucleotides in coenzymes, the existence of natural aptamers that bind these coenzymes, the existence of natural ribozymes, a biosynthetic pathway in which deoxynucleotides are produced from ribonucleotides, and the central role of ribosomal RNA in protein synthesis in the peptidyl transferase center of the ribosome. Here, we uses both a top-down approach that evaluates RNA function in modern biology and a bottom-up approach that examines the capacities of RNA independent of modern biology. These complementary approaches exploit multiple in vitro evolution techniques coupled with high-throughput sequencing and bioinformatics analysis. Together these complementary approaches advance our understanding of the most primitive organisms, their early evolution, and their eventual transition to modern biochemistry.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN13642 , Origins 2014 International Conference; Jul 06, 2014 - Jul 11, 2014; Nara; Japan
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  • 28
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    In:  CASI
    Publication Date: 2019-07-20
    Description: The Aviation Safety Reporting System (ASRS) in a partnership between the National Aeronautics and Space Administration (NASA), the Federal Aviation Administration (FAA), participating carriers, and labor organizations. It is designed to improve the National Airspace System by collecting and studying reports detailing unsafe conditions and events in the aviation industry. Employees are able to report safety issues or concerns with confidentiality and without fear of discipline. Safety reports highlighting fuel policies and alternate requirements for the aviation community highlight the human element in the complex aviation system.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN13826 , Aviation Safety InfoShare; Mar 04, 2014; Seattle, WA; United States
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  • 29
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    In:  CASI
    Publication Date: 2019-07-20
    Description: Project Overview: SporeSat is a fundamental space biology science space mission to investigate biophysical mechanisms of plant gravity sensing using a "lab-on-a-chip" experimental approach. The unicellular germinating Ceratopteris richardii fern spore will be studied in outer space. Science Objective: SporeSat shall determine gravity thresholds for calcium ion (Ca2+) channel activation in wild-fern spores. Why This is Important: Ion channels are critical to the functioning of biological organisms, including humans. Ion channels are key components of the nervous system as well as cardiac, skeletal, and smooth muscle function, transport of nutrients and ions, T-cell activation, and pancreatic beta-cell insulin release. Ion channels are often the target of the search for new drugs.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN15447 , Certamen Nacional "Misiones Espaciales Mexico"; May 29, 2014 - May 30, 2014; Mexico City; Mexico
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  • 30
    facet.materialart.
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    In:  CASI
    Publication Date: 2019-07-20
    Description: During spaceflight, astronauts experience weightlessness and are exposed to novel types of radiation. These environmental conditions may contribute to bone loss and reduction of structural integrity of the skeleton, which have negative implications for long-duration missions. The aim of this talk is to provide an overview of skeletal changes observed both in astronauts and in ground-based models of spaceflight, focusing on the fundamental biology and the prevention of deleterious skeletal changes.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN17133 , Ames Director''s Coloquim; Aug 05, 2014; Moffett Field, CA; United States
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  • 31
    Publication Date: 2019-07-20
    Description: DNA, RNA and proteins within a lipid-bound membrane are the core components of life, but the order of their appearance during the origin of life is still under debate. The widely accepted RNA World hypothesis states that RNA likely emerged prior to proteins and DNA since RNA can serve both replicative and catalytic roles. While biochemists have reproduced the synthesis, polymerization, and replication of nucleotides and RNA under controlled prebiotic conditions, it is clear that such complex organic molecules were are not present in significant amounts in the the starting prebiotic material on Earth either from endogenous production or meteoritic input. In contrast, amino acids are naturally abundant in various prebiotic contexts such as carbonaceous chondrites and Urey-Miller type experiments, and many studies have demonstrated that under plausible prebiotic conditions amino acids could condense or polymerize to give rise to short peptides. These findings support the basis of a Protein World hypothesis for life, however little has been done to study the functions of such primitive peptides. Here we present our novel synthetic biology-based approach to the de novo synthesis of billions of primitive peptidesproteins derived from a limited set of naturally abundant proteinogenic amino acids such as glycine, alanine, aspartic acid, glutamic acid, valine and serine. Of these peptides, the ones with divalent metal-binding capability are of particular interest and will be screened and identified. Certain divalent metals were likely present in prebiotic environments. Not only do they coordinate well with amino acids, but they also catalyze reactions, which are difficult to achieve in organic chemistry. Since D-chiral and non-proteinogenic amino acids are also abundant in the universe and may provide insight into the pathway by which life developed, we will also discuss methods to analyze primitive peptides consisting of these amino acids and D-chiral and non-proteinogenic amino acids. By understanding this naturalistic pathway, we will be able to better understand how life developed here on Earth. Since these amino acids are abundant in universe, this work provide insight into pathways by which life developed on Earth and, by extension, the probability of life arising elsewhere.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN13514 , Origins 2014; Jul 06, 2014 - Jul 11, 2014; Nara; Japan
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  • 32
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    In:  CASI
    Publication Date: 2019-07-20
    Description: The Aviation Safety Reporting System (ASRS) in a partnership between the National Aeronautics and Space Administration (NASA), the Federal Aviation Administration (FAA), participating carriers, and labor organizations. It is designed to improve the National Airspace System by collecting and studying reports detailing unsafe conditions and events in the aviation industry. Employees are able to report safety issues or concerns with confidentiality and without fear of discipline.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN13716 , Aviation Safety InfoShare; Mar 04, 2014; Seattle, WA; United States
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  • 33
    Publication Date: 2019-07-20
    Description: The Fruit Fly Lab is a hardware suite being designed to support research on the International Space Station (ISS) for use by the entire Drosophila research community. A validation mission will launch and return on SpaceX-5 in late 2014, followed by Principal Investigator-lead science flights thereafter. Space flight experiments are selected via peer-reviewed proposals open to the Drosophila community. The cassettes (containers) that will house the Drosophila cultures were successfully used to conduct an immunity study on the Space Shuttle. Results showed that the innate immune system of Drosophila melanogaster was affected by space flight with a reduction in phagocytosis function of plasmatocytes, changes in antimicrobial peptides and other gene expression levels, as well as changes in development of the animals. Scientific research topics that are of interest to NASA will be presented. Each cassette used to house the Drosophila has a removable food tray that can be replaced to sustain the growth of the culture, or can be transferred to another cassette, along with embryos and burrowed larvae, enabling multi-generational studies. The cassette can be frozen in the Minus Eighty Laboratory Freezer for ISS to preserve samples until post-flight analysis, expanding the applications of the hardware. Utilization of a centrifuge allows for on-orbit 1g controls for microgravity experiments, as well as variable g-levels for lunar or Mars environment studies. The standard form factor used also allows for implementation of modular upgrades. An observation system, circadian rhythm lighting system, and fixation capability are upgrades currently in development for near-term implementation. This hardware suite, with its flight- proven design and ability to utilize existing on-board facilities, offers the whole Drosophila research community a platform to address several key areas of the National Research Councils decadal survey, supporting the utilization of ISS for science discovery.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN13412 , Annual Drosophila Research Conference; Mar 26, 2014 - Mar 30, 2014; San Diego, CA; United States
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  • 34
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    In:  CASI
    Publication Date: 2019-07-12
    Description: The 2013 Nutrition Risk Standing Review Panel (from here on referred to as the SRP) met for a site visit in Houston, TX on November 20 - 21, 2013. The SRP reviewed the new Evidence Report for the Risk Factor of Inadequate Nutrition (from here on referred to as the 2013 Nutrition Evidence Report), as well as the Research Plan for this Risk. Overall, the SRP thinks the well-qualified research team has compiled an excellent summary of background information in the 2013 Nutrition Evidence Report. The SRP would like to commend the authors in general and particularly note that while the 2013 Nutrition Evidence Report has been written using a single nutrient approach, the research plan takes a much more integrated and physiologically based approach.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30328
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  • 35
    Publication Date: 2019-07-12
    Description: On December 5, 2013, the Pharmacology Risk SRP, participants from the JSC, HQ, the NSBRI, and NRESS participated in a WebEx/teleconference. The purpose of the call (as stated in the Statement of Task) was to allow the SRP members to: 1. Receive an update by the HRP Chief Scientist or Deputy Chief Scientist on the status of NASA's current and future exploration plans and the impact these will have on the HRP. 2. Receive an update on any changes within the HRP since the 2012 SRP meeting. 3. Receive an update by the Element or Project Scientist(s) on progress since the 2012 SRP meeting. 4. Participate in a discussion with the HRP Chief Scientist, Deputy Chief Scientist, and the Element regarding possible topics to be addressed at the next SRP meeting.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30327
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  • 36
    Publication Date: 2019-08-13
    Description: Based on previously reported procedures for permeabilizing vegetative bacterial cells, and numerous trial-and-error attempts with bacterial endospores, a protocol was developed for effectively permeabilizing bacterial spores, which facilitated the applicability of fluorescent in situ hybridization (FISH) microscopy. Bacterial endospores were first purified from overgrown, sporulated suspensions of B. pumilus SAFR-032. Purified spores at a concentration of approx equals 10 million spores/mL then underwent proteinase-K treatment, in a solution of 468.5 L of 100 mM Tris-HCl, 30 L of 10% SDS, and 1.5 microL of 20 mg/mL proteinase-K for ten minutes at 35 C. Spores were then harvested by centrifugation (15,000 g for 15 minutes) and washed twice with sterile phosphate-buffered saline (PBS) solution. This washing process consisted of resuspending the spore pellets in 0.5 mL of PBS, vortexing momentarily, and harvesting again by centrifugation. Treated and washed spore pellets were then resuspended in 0.5 mL of decoating solution, which consisted of 4.8 g urea, 3 mL Milli-Q water, 1 mL 0.5M Tris, 1 mL 1M dithiothreitol (DTT), and 2 mL 10% sodium-dodecylsulfate (SDS), and were incubated at 65 C for 15 minutes while being shaken at 165 rpm. Decoated spores were then, once again, washed twice with sterile PBS, and subjected to lysozyme/mutanolysin treatment (7 mg/mL lysozyme and 7U mutanolysin) for 15 minutes at 35 C. Spores were again washed twice with sterile PBS, and spore pellets were resuspended in 1-mL of 2% SDS. This treatment, facilitating inner membrane permeabilization, lasted for ten minutes at room temperature. Permeabilized spores were washed two final times with PBS, and were resuspended in 200 mkcroL of sterile PBS. At this point, the spores were permeable and ready for downstream processing, such as oligonucleotideprobe infiltration, hybridization, and microscopic evaluation. FISH-microscopic imagery confirmed the effective and efficient (50% successful permeabilization and recovery) permeabilization of numerous spore preparations. The novelty of the technology developed here is in its applicability to bacterial endospores. While protocols abound for the effective permeabilization of bacterial, archaeal, and eukaryotic vegetative cells, there are no such reliable methods for decoating and permeabilizing bacterial endospores in a manner that is amenable to downstream FISH microscopic analyses. This innovation enables the direct visualization and enumeration of spores via FISH-based microscopic techniques, circumventing the complications that accompany previously required germination regimes. The synergistic enzymatic weakening of the many spore layers facilitates a structural compromise that is just enough to render the spores permeable without degrading the spore to a level, which precludes it from recognition.
    Keywords: Life Sciences (General)
    Type: NPO-48035 , NASA Tech Briefs, January 2014; 13
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  • 37
    Publication Date: 2019-08-13
    Description: RNA isolation is a ubiquitous need, driven by current emphasis on microarrays and miniaturization. With commercial systems requiring 100,000 to 1,000,000 cells for successful isolation, there is a growing need for a small-footprint, easy-to-use device that can harvest nucleic acids from much smaller cell samples (1,000 to 10,000 cells). The process of extraction of RNA from cell cultures is a complex, multi-step one, and requires timed, asynchronous operations with multiple reagents/buffers. An added complexity is the fragility of RNA (subject to degradation) and its reactivity to surface. A novel, microfluidics-based, integrated cartridge has been developed that can fully automate the complex process of RNA isolation (lyse, capture, and elute RNA) from small cell culture samples. On-cartridge cell lysis is achieved using either reagents or high-strength electric fields made possible by the miniaturized format. Traditionally, silica-based, porous-membrane formats have been used for RNA capture, requiring slow perfusion for effective capture. In this design, high efficiency capture/elution are achieved using a microsphere-based "microfluidized" format. Electrokinetic phenomena are harnessed to actively mix microspheres with the cell lysate and capture/elution buffer, providing important advantages in extraction efficiency, processing time, and operational flexibility. Successful RNA isolation was demonstrated using both suspension (HL-60) and adherent (BHK-21) cells. Novel features associated with this development are twofold. First, novel designs that execute needed processes with improved speed and efficiency were developed. These primarily encompass electric-field-driven lysis of cells. The configurations include electrode-containing constructs, or an "electrode-less" chip design, which is easy to fabricate and mitigates fouling at the electrode surface; and the "fluidized" extraction format based on electrokinetically assisted mixing and contacting of microbeads in a shape-optimized chamber. A secondary proprietary feature is in the particular layout integrating these components to perform the desired operation of RNA isolation. Apart from a novel functional capability, advantages of the innovation include reduced or eliminated use of toxic reagents, and operator-independent extraction of RNA.
    Keywords: Life Sciences (General)
    Type: MSC-24375-1 , NASA Tech Briefs, January 2014; 13-14
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  • 38
    Publication Date: 2019-08-13
    Description: Long-term spaceflight causes profound changes to the musculoskeletal system attributable to unloading and fluid shifts in microgravity. Future space explorations beyond the earths magnetosphere will expose astronauts to space radiation, which may cause additional skeletal deficits that are not yet fully understood. Our long-term goals are twofold: to define the mechanisms and risk of bone loss in the spaceflight environment and to facilitate the development of effective countermeasures if necessary. Our central hypothesis is that oxidative stress plays a key role in progressive bone loss and vascular dysfunction caused by spaceflight. In animals models, overproduction of free radicals is associated with increased bone resorption, lower bone formation, and decrements in bone mineral density and structure which can ultimately lead to skeletal fragility. Evidence in support of a possible causative role for oxidative stress in spaceflight-induced bone loss derive from knockout and transgenic mouse studies and the use of pharmacological interventions with known anti-oxidant properties. In our studies to simulate spaceflight, 16-wk old, male C56Bl/6J mice were assigned to one of four groups: hind limb unloading to simulate weightlessness (HU), normally loaded Controls (NL) (sham irradiated, no hind limb unloading), irradiated at NASA Space Radiation Laboratory IR with 1-2Gy of (600MeV/n) alone, or in combination with protons (0.5Gy Protons/0.5Gy 56Fe), (IR) or both hind limb unloaded and irradiated, HU+IR. Mice were exposed to radiation 3 days after initiating HU and tissues harvested were 1-14 days after initiating treatments for analyses. Results from our laboratories, which employ various biochemical, gene expression, functional, and transgenic animal model methods, implicate dynamic regulation of redox-related pathways by spaceflight-related environmental factors. As one example, we found that combined HU and radiation exposure caused oxidative damage in skeletal tissues (lipid peroxidation) of wildtype mice, whereas bone from transgenic mice that overexpress human catalase in mitochondria were protected. Interestingly, marrow cells grown under culture conditions that select for endothelial progenitor cells (EPC), showed that HU but not IR reduced EPC cell migration; in contrast HU and IR each inhibited growth of marrow-derived osteoblast progenitors. Taken together, these results indicate that unloading and ionizing elicit distinct effects on progenitor and mature cells of vascular and skeletal tissue, and that oxidative damage may contribute to skeletal and vascular deficits that may emerge during extended space travel.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN18527 , Human Research Program Investigators'' Workshop; Feb 12, 2014 - Feb 13, 2014; Galveston, TX; United States
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  • 39
    Publication Date: 2019-08-13
    Description: Research using rodents is an essential tool for advancing biomedical research on Earth and in space. The National Research Counsels Decadal survey (1) emphasized the importance of expanding NASAs life sciences research to perform long duration, rodent experiments on the International Space Station (ISS). To accomplish this objective, flight hardware, operations, and science capabilities were developed at NASA ARC to support both commercial and government-sponsored research. In preparation for the maiden voyage of the Rodent Habitat hardware and operations system (Rodent Research-1), and in close consultation with a Science Working Group comprised of veterinarians and experienced spaceflight investigators, we modified existing Animal Enclosure Module hardware, developed new hardware, operations, and science activities, and performed a series of ground-based verification tests. Preflight, ground based hardware tests included a simulation of SpaceX Dragon launch conditions (vibration and hypergravity) using the Transporter, and also two long-term biocompatibility tests (32 and 92 days) using the Habitat developed for long term housing on the ISS. The launch simulation test showed that adult mice housed in Transporter hardware adapted well, even if launch simulation was followed by a period of simulated weightlessness (via hind limb unloading). The biocompatibility tests demonstrated that the Habitat successfully supported animal health and also provided a useful video imaging system that enables frequent monitoring of animal health and behavior by veterinary and scientific experts on the ground, independent of ISS crew intervention. At the conclusion of all tests, mice were deemed healthy and suitable for conducting biological research. Additional preflight analyses of tissues preserved by freezing or fixation for gene expression analyses revealed that spleen and liver tissues recovered under conditions that simulated on-orbit activities yielded high quality RNA (RIN values 8-10) and liver enzyme activities and protein content (e.g. catalase). In addition, new methods were developed to optimize future science return by dissecting tissues post-euthanasia and storage. Various tissues were harvested from either intact or partially dissected, frozen carcasses after storage for ~2-6 months; most of the tissues (brain, heart, kidney, eye, adrenal glands and skeletal muscle) were of high RNA quality for science return, whereas some tissues (small intestine, bone marrow and bones) were not. These data demonstrated the protocols developed for future flight experiments supported science return despite delayed preservation post-euthanasia or prolonged storage, and furthermore, that high-quality RNA samples from many different tissues can be recovered by dissection following prolonged storage of the tissue in situ at -80C. The first flight experiments carrying 20 mice were launched on Sept 21, 2014 in an unmanned Dragon Capsule, SpaceX4; Rodent Research-1 is dedicated to achieving both NASA validation and CASIS science objectives. Ground based control groups (housed in flight hardware or standard cages) were maintained in environmental chambers at Kennedy Space Center. Crewmembers previously trained in animal handling transferred mice from the Transporter into Habitats under simultaneous veterinary supervision by video streaming and were deemed healthy. Health and behavior of all mice on the ISS was monitored by video feed on a daily basis. The 10 mice for validation (16wk old, female C57Bl6/J) ambulated freely and actively throughout the Habitat, relying heavily on their forelimbs for locomotion. The first on-orbit dissections of mice were performed successfully on Oct 12 and 13, 2014, and the validation mice will reside on ISS for up to 30 days. In conclusion, new capability for long duration rodent research is under development, including in-flight sample collection (which avoids the complication of reentry); results obtained to date will be described. This new Rodent Research system enables achievement of both basic science and translational research objectives to advance human exploration of space.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN18479 , Human Research Program Investigators Workshop; Feb 12, 2014 - Feb 13, 2014; Galveston, TX; United States
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  • 40
    Publication Date: 2019-08-13
    Description: Long-term spaceflight leads to extensive changes in the musculoskeletal system attributable, in part, to unloading during microgravity exposure. Additionally, irradiation at doses similar to that of a solar flare or a round-trip sojourn to Mars may cause significant depletion of stem/progenitor cell pools throughout the body as well as inflammation associated with prompt skeletal-tissue degradation. Previously, we demonstrated that irradiation leads to rapid bone loss, which can be mitigated in the short term by injection of a potent antioxidant (-lipoic acid). Furthermore, simulated weightlessness in adult mice adversely affects skeletal responses to low linear energy transfer (LET) radiation (137Cs). Here, we hypothesized that simulated weightlessness exacerbates the adverse effects of simulated space radiation (including both protons and 56Fe ions) by adversely affecting skeletal structure and functions as well as associated vasculature. Furthermore, we hypothesized that an antioxidant cocktail, which has been shown to be protective in other tissues, mitigates space radiation induced bone loss.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN12029 , NASA Human Research Program Investigators'' Workshop (HRP 2014); Feb 12, 2014 - Feb 13, 2014; Glaveston, TX; United States
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  • 41
    Publication Date: 2019-08-13
    Description: Alterations and impairment of immune responses in humans present a health risk for space exploration missions. The molecular mechanisms under pinning innate immune defense can be confounded by the complexity of the acquired immune system of humans. Drosophila (fruit fly) innate immunity is simpler, and shares many similarities with human innate immunity at the level of molecular and genetic pathways. The goals of this study were to elucidate fundamental immune processes in Drosophila affected by spaceflight and to measure host-pathogen responses post-flight. Five containers, each containing ten female and five male fruit flies, were housed and bred on the space shuttle (average orbit altitude of330.35 km) for 12 days and 18.5 hours. A new generation of flies was reared in microgravity. In larvae, the immune system was examined by analyzing plasmatocyte number and activity in culture. In adults, the induced immune responses were analyzed by bacterial clearance and quantitative real-time polymerase chain reaction (qPCR) of selected genes following infection with E. coli. The RNA levels of relevant immune pathway genes were determined in both larvae and adults by microarray analysis. The ability of larval plasmatocytes to phagocytose E. coli in culture was attenuated following spaceflight, and in parallel, the expression of genes involved in cell maturation was down regulated. In addition, the level of constitutive expression of pattern recognition receptors and opsonins that specifically recognize bacteria, and of lysozymes, antimicrobial peptide (AMP) pathway and immune stress genes, hallmarks of humoral immunity, were also reduced in larvae. In adults, the efficiency of bacterial clearance measured in vivo following a systemic infection with E. coli post-flight, remained robust. We show that spaceflight altered both cellular and humoral immune responses in Drosophila and that the disruption occurs at multiple interacting pathways.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN12039 , NASA Human Research Program Investigators'' Workshop (HRP 2014); Feb 12, 2014 - Feb 13, 2014; Glaveston, TX; United States
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  • 42
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    In:  CASI
    Publication Date: 2019-08-28
    Description: A method of measuring motion blur is disclosed comprising obtaining a moving edge temporal profile r(sub 1)(k) of an image of a high-contrast moving edge, calculating the masked local contrast m(sub1)(k) for r(sub 1)(k) and the masked local contrast m(sub 2)(k) for an ideal step edge waveform r(sub 2)(k) with the same amplitude as r(sub 1)(k), and calculating the measure or motion blur Psi as a difference function, The masked local contrasts are calculated using a set of convolution kernels scaled to simulate the performance of the human visual system, and Psi is measured in units of just-noticeable differences.
    Keywords: Life Sciences (General)
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  • 43
    Publication Date: 2019-07-12
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: JSC-CN-32010
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  • 44
    Publication Date: 2019-07-12
    Description: A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.
    Keywords: Life Sciences (General)
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  • 45
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    In:  CASI
    Publication Date: 2019-07-12
    Description: Provided herein are an isolated or enriched population of tumor initiating cells derived from normal cells, cells susceptible to neoplasia, or neoplastic cells. Methods of use of the cells for screening for anti-hyperproliferative agents, and use of the cells for animal models of hyperproliferative disorders including metastatic cancer, diagnostic methods, and therapeutic methods are provided.
    Keywords: Life Sciences (General)
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  • 46
    Publication Date: 2019-07-12
    Description: Recently, a parallel pathway model to describe ankle dynamics was proposed. This model provides a relationship between ankle angle and net ankle torque as the sum of a linear and nonlinear contribution. A technique to identify parameters of this model in discrete-time has been developed. However, these parameters are a nonlinear combination of the continuous-time physiology, making insight into the underlying physiology impossible. The stable and accurate estimation of continuous-time parameters is critical for accurate disease modeling, clinical diagnosis, robotic control strategies, development of optimal exercise protocols for longterm space exploration, sports medicine, etc. This paper explores the development of a system identification technique to estimate the continuous-time parameters of ankle dynamics. The effectiveness of this approach is assessed via simulation of a continuous-time model of ankle dynamics with typical parameters found in clinical studies. The results show that although this technique improves estimates, it does not provide robust estimates of continuous-time parameters of ankle dynamics. Due to this we conclude that alternative modeling strategies and more advanced estimation techniques be considered for future work.
    Keywords: Life Sciences (General)
    Type: NASA/TM-2014-218314 , DFRC-E-DAA-TN14535
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  • 47
    Publication Date: 2019-07-12
    Description: Mitigate microbial risk to crew health, safety, and performance during the human exploration of space
    Keywords: Life Sciences (General)
    Type: JSC-CN-31814
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  • 48
    Publication Date: 2019-07-12
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: JSC-CN-31812
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  • 49
    Publication Date: 2019-07-12
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: JSC-CN-31683
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  • 50
    Publication Date: 2019-07-12
    Description: The present invention provides a method of forming a blood-clot microvalve by heating blood in a capillary tube of a microfluidic device. Also described are methods of modulating liquid flow in a capillary tube by forming and removing a blood-clot microvalve.
    Keywords: Life Sciences (General)
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  • 51
    Publication Date: 2019-07-13
    Description: Photosynthetic and growth data were collected on APH Root Module. Described Stand pipe system for active moisture control. Tested germination in wicks. Evaluated EC-5 moisture sensors. Demonstrated that Wheat plants can grow in the APH Root Module.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN18441 , American Society for Gravitational and Space Research; Oct 22, 2014 - Oct 26, 2014; Pasadena CA; United States
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  • 52
    Publication Date: 2019-07-13
    Description: The lunar surface is covered by a layer of fine, reactive dust. Very little is known regarding the toxicity of lunar dust on human physiology. This study assessed the toxicity of airborne lunar dust exposure in rats on pulmonary and systemic immune parameters.
    Keywords: Life Sciences (General)
    Type: JSC-CN-31994 , Annual Clinical Cytometry Meeting & Course; Oct 10, 2014 - Oct 14, 2014; Seattle, WA; United States
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  • 53
    Publication Date: 2019-07-13
    Description: Presentation of the status of the Seedling Growth ISS Payloads to the Payload Operations Investigator Working Group meeting. The meeting will be held at MSFC, Huntsville AL, January 27-31, 2014.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN12782 , Payload Operations Investigator Working Group Face To Face Meeting; Jan 27, 2014 - Jan 31, 2014; Huntsville, Alabama; United States
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  • 54
    Publication Date: 2019-07-13
    Description: The purpose of this study was to identify and optimize fast and reliable sampling and detection methods for the identification of pathogens that may be present on produce grown in small vegetable production units on the International Space Station (ISS), thus a field setting. Microbiological testing is necessary before astronauts are allowed to consume produce grown on ISS where currently there are two vegetable production units deployed, Lada and Veggie.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN14974 , General Meeting for American Society of Microbiology; May 17, 2014 - May 20, 2014; Boston MA; United States
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  • 55
    Publication Date: 2019-07-13
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: JSC-CN-31352 , International Society for Gravitational Physiology (ISGP) 2014 Conference; Jun 15, 2014 - Jun 20, 2014; Waterloo, Toronto; Canada
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  • 56
    facet.materialart.
    Unknown
    In:  CASI
    Publication Date: 2019-07-13
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: JSC-CN-31094 , Telemedicine (JSC Connect 2014); May 08, 2014; Houston, TX; United States
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  • 57
    Publication Date: 2019-07-13
    Description: The formation of doublestrand breaks (DSBs) and chromosomal aberrations (CAs) is of great importance in radiation research and, specifically, in space applications. We are presenting a new particle track and DNA damage model, in which the particle stochastic track structure is combined with the random walk (RW) structure of chromosomes in a cell nucleus. The motivation for this effort stems from the fact that the model with the RW chromosomes, NASARTI (NASA radiation track image) previously relied on amorphous track structure, while the stochastic track structure model RITRACKS (Relativistic Ion Tracks) was focused on more microscopic targets than the entire genome. We have combined chromosomes simulated by RWs with stochastic track structure, which uses nanoscopic dose calculations performed with the MonteCarlo simulation by RITRACKS in a voxelized space. The new simulations produce the number of DSBs as function of dose and particle fluence for highenergy particles, including iron, carbon and protons, using voxels of 20 nm dimension. The combined model also calculates yields of radiationinduced CAs and unrejoined chromosome breaks in normal and repair deficient cells. The joined computational model is calibrated using the relative frequencies and distributions of chromosomal aberrations reported in the literature. The model considers fractionated deposition of energy to approximate dose rates of the space flight environment. The joined model also predicts of the yields and sizes of translocations, dicentrics, rings, and more complextype aberrations formed in the G0/G1 cell cycle phase during the first cell division after irradiation. We found that the main advantage of the joined model is our ability to simulate small doses: 0.050.5 Gy. At such low doses, the stochastic track structure proved to be indispensable, as the action of individual deltarays becomes more important.
    Keywords: Life Sciences (General)
    Type: JSC-CN-31001 , Radiation Research Society Conference; Sep 21, 2014 - Sep 24, 2014; Las Vegas, NV; United States
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  • 58
    Publication Date: 2019-07-13
    Description: Tree fruit, although desirable from a crew nutrition and menu diversity perspective, have long been dismissed as candidate crops based on their long juvenile phase, large architecture, low short-term harvest index, and dormancy requirements. Recent developments in Rapid Cycle Crop Breeding (RCCB) have overcome these historical limitations, opening the door to a new era in candidate crop research. Researchers at the United States Department of Agriculture (USDA) have developed FT-construct (Flowering Locus T) dwarf plum lines that have a very short juvenile phase, vine-like architecture, and no obligate dormancy period. In a collaborative research effort, NASA and the USDA are evaluating the performance of these FT-lines under controlled environment conditions relevant to spaceflight.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN14905 , AgroSpace; May 22, 2014 - May 23, 2014; Sperlonga, Italy; Italy
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  • 59
    Publication Date: 2019-07-13
    Description: This presentation will review the current capacity and capabilities of the NASA Kennedy Spacer Center's Laboratories.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN14376 , International Workshop on Lunar Science Applications; Apr 08, 2014 - Apr 11, 2014; Cocoa Beach, Florida; United States
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  • 60
    Publication Date: 2019-07-13
    Description: NASA and other space agencies have an interest in using plants for human life support in space. The plants could provide food and O2 for the humans, while removing CO2 and helping purify wastewater. Studies to date have shown that a wide range of crops can be grown in controlled environment conditions envisioned for space. Light is a critical factor both for crop productivity and system power costs, and recent improvements in LEDs make them a preferred lighting option for space. Because space systems would be tightly closed, issues such as ethylene build-up and management must be considered. Ultimately, the costs and reliability of biological life support options must be compared with more conventional life support approaches. Findings to date suggest that about 20-25 sq. meters of crops could supply the O2 for one human, while about 50 sq. meters would be required for food (dietary calories).
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN18592 , International Conference on Plant Factory (ICPF); Nov 10, 2014 - Nov 12, 2014; Kyoto; Japan
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  • 61
    Publication Date: 2019-07-13
    Description: This is our annual report to the North Central Extension Research Activity, which is affiliated with the USDA and Land Grant University Agricultural Experiment Stations. I have been a member of this committee for 25 years. The presentation will be given by Dr. Gioia Massa, Kennedy Space Center
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN14350 , NCERA-101 Annual Meeting; Apr 03, 2014; Fairbanks, Alaska; United States
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  • 62
    Publication Date: 2019-11-09
    Description: The skeleton interacts with its environment in a way that resembles a mechanostat - through a controlled process of bone remodeling, namely local formation and resorption, to maintain a healthy structure. During weightlessness, astronauts lose structure in weight-bearing bones due to decreased formation by osteoblasts and increased resorption by osteoclasts. In contrast, increased mechanical loading through exercise targets bone remodeling to remove and repair microdamage, improving structural integrity. In fact, recent advances in astronaut exercise regimens have prevented the deleterious changes in skeletal structure during spaceflight. However, knowledge of the molecular underpinnings of the skeletal response to spaceflight and to mechanical stimulation is limited. We propose that epigenetic modification, specifically DNA methylation, may influence osteoblast differentiation and activity during spaceflight and exercise. We hypothesize that simulated weightlessness hypermethylates pro-osteoblastogenic gene promoters and decreases expression of osteoblastogenic genes. Oppositely, we hypothesize that mechanical loading hypomethylates pro-osteoblastogenic gene promoters and increases expression of osteoblastogenic genes.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN16021 , Annual Meeting of the American Society for Gravitational and Space Research; Oct 22, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 63
    Publication Date: 2019-08-14
    Description: The E. coli AntiMicrobial Satellite(EcAMSat) mission will investigate space microgravity affects on the antibiotic resistance of E. coli, a bacterial pathogen responsible for urinary tract infection in humans and animals. EcAMSat is being developed through a partnership between NASA Ames Research Center and the Stanford University School of Medicine. Scientists believe that the results of this experiment could help design effective countermeasures to protect astronauts health during long duration human space missions.
    Keywords: Life Sciences (General)
    Type: EcAMSat-FS-061214 , FS #2014-06-02-ARC , ARC-E-DAA-TN15812
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  • 64
    facet.materialart.
    Unknown
    In:  CASI
    Publication Date: 2019-07-12
    Description: Solid-state nanopore-based analysis of nucleic acid polymers is revolutionary. No other technique can determine information content in single molecules of genetic material at the speed of 1 subunit per microsecond. Because individual molecules are counted, the output is intrinsically quantitative. The nanopore approach is more generalized than any other method and in principle may be used to analyze any polymer molecule, including proteins. The approach to the development of a solid-state nanopore device is novel in the use of nanofabrication, nanoelectronic components, and high-speed signal acquisition. A novel geometry of the solid-state nanopore (less than 5 nm in length and 5 nm in diameter) will enable 1 to 5 nucleotide resolution measurements. This means that maximum resolution will be improved at least 100-fold compared to biological ion-channel measurements. The solid-state nanopore sensor will be made to enable sequencing DNA at a much faster rate than presently possible without the need for extensive sample preparation procedures, such as enzymatic amplification and labeling reactions. It will analyze electronic properties of individual subunits of DNA or RNA, to obtain linear composition of each genetic polymer molecule.
    Keywords: Life Sciences (General)
    Type: NASA FS-2014-01-01-ARC , ARC-E-DAA-TN13613
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  • 65
    Publication Date: 2019-07-12
    Description: The process of water purification has many different physical, chemical, and biological processes. One part of the biological process is the task of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB). Both play critical roles in the treatment of wastewater by oxidizing toxic compounds. The broad term is nitrification, a naturally occurring process that is carried out by AOB and NOB by using oxidation to convert ammonia to nitrite and nitrite to nitrate. To monitor this biological activity, bacterial staining was performed on wastewater contained in inoculum tanks and biofilm samples from bioreactors. Using microscopy and qPCR, the purpose of this experiment was to determine if the population of AOB and NOB in wastewater and membrane bioreactors changed depending on temperature and hibernation conditions to determine the optimal parameters for AOB/NOB culture to effectively clean wastewater.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN17140
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  • 66
    Publication Date: 2019-07-12
    Description: Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.
    Keywords: Life Sciences (General)
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  • 67
    Publication Date: 2019-07-12
    Description: The present invention relates to a method of detecting coliform bacteria in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.
    Keywords: Life Sciences (General)
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  • 68
    Publication Date: 2019-08-26
    Description: The present invention is directed to methods of manufacturing bioactive gels from ECM material, i.e., gels which retain bioactivity, and can serve as scaffolds for preclinical and clinical tissue engineering and regenerative medicine approaches to tissue reconstruction. The manufacturing methods take advantage of a new recognition that bioactive gels from ECM material can be created by digesting particularized ECM material in an alkaline environment and neutralizing to provide bioactive gels.
    Keywords: Life Sciences (General)
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  • 69
    Publication Date: 2019-07-19
    Description: Human exploration off planet is severely limited by the cost of launching materials into space and re-supply. Thus materials brought from earth must be light, stable and reliable at destination. Using traditional approaches a lunar or Mars base would require either transporting a hefty store of metals or heavy manufacturing equipment and construction materials for in situ extraction; both would severely limit any other mission objectives. Long-term human space presence requires periodic replenishment, adding a massive cost overhead. Even robotic missions often sacrifice science goals for heavy radiation and thermal protection. Biology has the potential to solve these problems because it can replicate and repair itself, and do a wide variety of chemical reactions including making food, fuel and materials. Synthetic biology can greatly enhance and expand life's evolved repertoire. Using natural and synthetically altered organisms as the feedstock for additive manufacturing could one day make possible the dream of producing bespoke tools, food, smart fabrics and even replacement organs on demand. To this end our lab has produced a proof-of-concept bioprinter with nearly one-cell resolution. Genetically engineering yeast cells to secrete bioproducts subsequent to printing allows the potential to make biomaterials with a fine microstructure. Imagine a production system that, at a few micron scale resolution, can add mollusk shell for compressive strength per unit mass, spider silk or collagen for tensile strength per unit mass, and potentially biologically-deposited wires. Now imagine what new products can be enabled by such a technology, on earth or beyond.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN18464 , Inside 3D Printing Conference; Oct 21, 2014 - Oct 23, 2014; Santa Clara, CA; United States
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  • 70
    Publication Date: 2019-07-19
    Description: During prolonged spaceflight, astronauts are exposed to both microgravity and space radiation, and are at risk for increased skeletal fragility due to bone loss. Evidence from rodent experiments demonstrates that both microgravity and ionizing radiation can cause bone loss due to increased bone-resorbing osteoclasts and decreased bone-forming osteoblasts, although the underlying molecular mechanisms for these changes are not fully understood. We hypothesized that excess reactive oxidative species (ROS), produced by conditions that simulate spaceflight, alter the tight balance between osteoclast and osteoblast activities, leading to accelerated skeletal remodeling and culminating in bone loss. To test this, we used the MCAT mouse model; these transgenic mice over-express the human catalase gene targeted to mitochondria, the major organelle contributing free radicals. Catalase is an anti-oxidant that converts reactive species, hydrogen peroxide into water and oxygen. This animal model was selected as it displays extended lifespan, reduced cardiovascular disease and reduced central nervous system radio-sensitivity, consistent with elevated anti-oxidant activity conferred by the transgene. We reasoned that mice overexpressing catalase in mitochondria of osteoblast and osteoclast lineage cells would be protected from the bone loss caused by simulated spaceflight. Over-expression of human catalase localized to mitochondria caused various skeletal phenotypic changes compared to WT mice; this includes greater bone length, decreased cortical bone area and moment of inertia, and indications of altered microarchitecture. These findings indicate mitochondrial ROS are important for normal bone-remodeling and skeletal integrity. Catalase over-expression did not fully protect skeletal tissue from structural decrements caused by simulated spaceflight; however there was significant protection in terms of cellular oxidative damage (MDA levels) to the skeletal tissue. Furthermore, we used an array of countermeasures (Antioxidant diets and injections) to prevent the radiation-induced bone loss, although these did not prevent bone loss, analysis is ongoing to determine if these countermeasure protected radiation-induced damage to other tissues.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN16027 , Annual Meeting of UK''s Association for Radiation Research; Jun 29, 2014 - Jul 02, 2014; Sussex, Brighton; United Kingdom
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  • 71
    Publication Date: 2019-07-19
    Description: Exposure to musculoskeletal disuse and radiation result in bone loss; we hypothesized that these catabolic treatments cause excess reactive oxygen species (ROS), and thereby alter the tight balance between bone resorption by osteoclasts and bone formation by osteoblasts, culminating in bone loss. To test this, we used transgenic mice which over-express the human gene for catalase, targeted to mitochondria (MCAT). Catalase is an anti-oxidant that converts the ROS hydrogen peroxide into water and oxygen. MCAT mice were shown previously to display reduced mitochondrial oxidative stress and radiosensitivity of the CNS compared to wild type controls (WT). As expected, MCAT mice expressed the transgene in skeletal tissue, and in marrow-derived osteoblasts and osteoclast precursors cultured ex vivo, and also showed greater catalase activity compared to wildtype (WT) mice (3-6 fold). Colony expansion in marrow cells cultured under osteoblastogenic conditions was 2-fold greater in the MCAT mice compared to WT mice, while the extent of mineralization was unaffected. MCAT mice had slightly longer tibiae than WT mice (2%, P less than 0.01), although cortical bone area was slightly lower in MCAT mice than WT mice (10%, p=0.09). To challenge the skeletal system, mice were treated by exposure to combined disuse (2 wk Hindlimb Unloading) and total body irradiation Cs(137) (2 Gy, 0.8 Gy/min), then bone parameters were analyzed by 2-factor ANOVA to detect possible interaction effects. Treatment caused a 2-fold increase (p=0.015) in malondialdehyde levels of bone tissue (ELISA) in WT mice, but had no effect in MCAT mice. These findings indicate that the transgene conferred protection from oxidative damage caused by treatment. Unexpected differences between WT and MCAT mice emerged in skeletal responses to treatment.. In WT mice, treatment did not alter osteoblastogenesis, cortical bone area, moment of inertia, or bone perimeter, whereas in MCAT mice, treatment increased these parameters. Taken together, this typically catabolic treatment (disuse and irradiation) appeared to stimulate cortical expansion in MCAT mice but not WT mice. In conclusion, these results reveal the importance of mitochondrial ROS generation in skeletal remodeling and show that MCAT mice provide a useful animal model for bone studies.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN14267 , Annual Meeting of the Radiation Research Society; Sep 21, 2014 - Sep 24, 2014; Las Vegas, NV; United States
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  • 72
    Publication Date: 2019-07-19
    Description: A desired architecture for long duration spaceflight, like aboard the International Space Station or for future missions to Mars, is to provide a supply of fresh food crops for the astronauts. However, some crops can create a high proportion of inedible plant waste. The main goal of the Synthetic Biology project, Cow in a Column, was to produce the components of milk (sugar, lipid, protein) from inedible plant waste by utilizing microorganisms (fungi, yeast, bacteria). Of particular interest was utilizing the valuable polysaccharide, cellulose, found in plant waste, to naturally fuel-through microorganism cellular metabolism- the creation of sugar (glucose), lipid (milk fat), and protein (casein) in order to produce a synthetic edible food product. Environmental conditions such as pH, temperature, carbon source, aeration, and choice microorganisms were optimized in the laboratory and the desired end-products, sugars and lipids, were analyzed. Trichoderma reesei, a known cellulolytic fungus, was utilized to drive the production of glucose, with the intent that the produced glucose would serve as the carbon source for milk fat production and be a substitute for the milk sugar lactose. Lipid production would be carried out by Rhodosporidium toruloides, yeast known to accumulate those lipids that are typically found in milk fat. Results showed that glucose and total lipid content were below what was expected during this phase of experimentation. In addition, individual analysis of six fatty acids revealed that the percentage of each fatty acid was lower than naturally produced bovine milk. Overall, this research indicates that microorganisms could be utilized to breakdown inedible solid waste to produce useable products. For future work, the production of the casein protein for milk would require the development of a genetically modified organism, which was beyond the scope of the original project. Additional trials would be needed to further refine the required environment/organisms for the production of desired sugar and lipid end-products.
    Keywords: Life Sciences (General)
    Type: KSC-E-DAA-TN12681 , International Conference on Environmental Systems (ICES 2014); Jul 13, 2014 - Jul 17, 2014; Tuscon, Az; United States
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  • 73
    Publication Date: 2019-07-19
    Description: The objective of NASA Ames Research Centers WetLab-2 Project is to place on the ISS a research platform capable of conducting gene expression analysis via quantitative real-time PCR (qRT-PCR) of biological specimens sampled or cultured on orbit. The project has selected a Commercial-Off-The-Shelf (COTS) qRT-PCR system, the Cepheid SmartCycler and will fly it in its COTS configuration. The SmartCycler has a number of advantages including modular design (16 independent PCR modules), low power consumption, rapid ramp times and the ability to detect up to four separate fluorescent channels at one time enabling multiplex assays that can be used for normalization and to study multiple genes of interest in each module. The team is currently working with Cepheid to enable the downlink of data from the ISS to the ground and provide uplink capabilities for programming, commanding, monitoring, and instrument maintenance. The project has adapted commercial technology to design a module that can lyse cells and extract RNA of sufficient quality and quantity for use in qRT-PCR reactions while using a housekeeping gene to normalize RNA concentration and integrity. The WetLab-2 system is capable of processing multiple sample types ranging from microbial cultures to animal tissues dissected on-orbit. The ability to conduct qRT-PCR on-orbit eliminates the confounding effects on gene expression of reentry stresses and shock acting on live cells and organisms or the concern of RNA degradation of fixed samples. The system can be used to validate terrestrial analyses of samples returned from ISS by providing on-orbit gene expression benchmarking prior to sample return. The ability to get on orbit data will provide investigators with the opportunity to adjust experiment parameters for subsequent trials based on the real-time data analysis without need for sample return and re-flight. Researchers will also be able to sample multigenerational changes in organisms. Finally, the system can be used for analysis of air, surface, water, and clinical samples to monitor environmental contaminants and crew health. The verification flight of the instrument is scheduled to launch on SpaceX-7 in June 2015.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN15977 , Annual Meeting of the American Society for Gravitational and Space Research; Oct 22, 2014 - Oct 26, 2014; Pasadena, CA; United States
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  • 74
    Publication Date: 2019-07-19
    Description: The formation of DNA double-strand breaks (DSBs) and chromosome aberrations is an important consequence of ionizing radiation. To simulate DNA double-strand breaks and the formation of chromosome aberrations, we have recently merged the codes RITRACKS (Relativistic Ion Tracks) and NASARTI (NASA Radiation Track Image). The program RITRACKS is a stochastic code developed to simulate detailed event-by-event radiation track structure: [1] This code is used to calculate the dose in voxels of 20 nm, in a volume containing simulated chromosomes, [2] The number of tracks in the volume is calculated for each simulation by sampling a Poisson distribution, with the distribution parameter obtained from the irradiation dose, ion type and energy. The program NASARTI generates the chromosomes present in a cell nucleus by random walks of 20 nm, corresponding to the size of the dose voxels, [3] The generated chromosomes are located within domains which may intertwine, and [4] Each segment of the random walks corresponds to approx. 2,000 DNA base pairs. NASARTI uses pre-calculated dose at each voxel to calculate the probability of DNA damage at each random walk segment. Using the location of double-strand breaks, possible rejoining between damaged segments is evaluated. This yields various types of chromosomes aberrations, including deletions, inversions, exchanges, etc. By performing the calculations using various types of radiations, it will be possible to obtain relative biological effectiveness (RBE) values for several types of chromosome aberrations.
    Keywords: Life Sciences (General)
    Type: JSC-CN-31307 , International Symposium of Chromosomal Aberrations (ISCA11); Sep 12, 2014 - Sep 14, 2014; Rhodes; Greece
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  • 75
    Publication Date: 2019-07-19
    Description: The formation of doublestrand breaks (DSBs) and chromosomal aberrations (CAs) is of great importance in radiation research and, specifically, in space applications. We are presenting a new particle track and DNA damage model, in which the particle stochastic track structure is combined with the random walk (RW) structure of chromosomes in a cell nucleus. The motivation for this effort stems from the fact that the model with the RW chromosomes, NASARTI (NASA radiation track image) previously relied on amorphous track structure, while the stochastic track structure model RITRACKS (Relativistic Ion Tracks) was focused on more microscopic targets than the entire genome. We have combined chromosomes simulated by RWs with stochastic track structure, which uses nanoscopic dose calculations performed with the MonteCarlo simulation by RITRACKS in a voxelized space. The new simulations produce the number of DSBs as function of dose and particle fluence for highenergy particles, including iron, carbon and protons, using voxels of 20 nm dimension. The combined model also calculates yields of radiationinduced CAs and unrejoined chromosome breaks in normal and repair deficient cells. The joined computational model is calibrated using the relative frequencies and distributions of chromosomal aberrations reported in the literature. The model considers fractionated deposition of energy to approximate dose rates of the space flight environment. The joined model also predicts of the yields and sizes of translocations, dicentrics, rings, and more complextype aberrations formed in the G0/G1 cell cycle phase during the first cell division after irradiation. We found that the main advantage of the joined model is our ability to simulate small doses: 0.050.5 Gy. At such low doses, the stochastic track structure proved to be indispensable, as the action of individual deltarays becomes more important.
    Keywords: Life Sciences (General)
    Type: JSC-CN-31001 , Annual International Meeting of the Radiation Research Society; Sep 21, 2014 - Sep 24, 2014; Las Vegas, NV; United States
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  • 76
    Publication Date: 2019-07-19
    Description: When describing human motion, biomechanists generally report joint angles in terms of Euler angle rotation sequences. However, there are known limitations in using this method to describe complex motions such as the shoulder joint during a baseball pitch. Euler angle notation uses a series of three rotations about an axis where each rotation is dependent upon the preceding rotation. As such, the Euler angles need to be regarded as a set to get accurate angle information. Unfortunately, it is often difficult to visualize and understand these complex motion representations. One of our key functions is to help design engineers understand how a human will perform with new designs and all too often traditional use of Euler rotations becomes as much of a hindrance as a help. It is believed that using a spherical coordinate system will allow ABF personnel to more quickly and easily transmit important mobility data to engineers, in a format that is readily understandable and directly translatable to their design efforts. Objectives: The goal of this project is to establish new analysis and visualization techniques to aid in the examination and comprehension of complex motions. Methods: This project consisted of a series of small subprojects, meant to validate and verify the method before it was implemented in the ABF's data analysis practices. The first stage was a proof of concept, where a mechanical test rig was built and instrumented with an inclinometer, so that its angle from horizontal was known. The test rig was tracked in 3D using an optical motion capture system, and its position and orientation were reported in both Euler and spherical reference systems. The rig was meant to simulate flexion/extension, transverse rotation and abduction/adduction of the human shoulder, but without the variability inherent in human motion. In the second phase of the project, the ABF estimated the error inherent in a spherical coordinate system, and evaluated how this error would vary within the reference frame. This stage also involved expanding a kinematic model of the shoulder, to include the torso, knees, ankle, elbows, wrists and neck. Part of this update included adding a representation of 'roll' about an axis, for upper arm and lower leg rotations. The third stage of the project involved creating visualization methods to assist in interpreting motion in a spherical frame. This visualization method will be incorporated in a tool to evaluate a database of suited mobility data, which is currently in development.
    Keywords: Life Sciences (General)
    Type: JSC-CN-30931
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  • 77
    Publication Date: 2019-07-13
    Description: Mechanical unloading of muscle during spaceflight in microgravity is known to cause muscular atrophy, changes in muscle fiber type composition, gene expression, and reductions in regenerative muscle growth. Although limited data exists for long-term effects of microgravity in human muscle, these processes have mostly been studied in rodents for short periods of time. Here we report on how 30-day, long-term, mechanical unloading in microgravity affects mouse muscle of the femoral Quadriceps group. To conduct these studies we used muscle tissue from 6 mice from the NASA Biospecimen Sharing Program conducted in collaboration with the Institute for Biomedical Problems of the Russian Academy of Sciences, during the Russian Bion M1 biosatellite mission in 2013. Muscle morphology observed in histological sections shows signs of extensive atrophy and regenerative hypoplasia. Specifically, we observed a two-fold decrease in the number of myonuclei, their central location, low density of myofibers and myofibrils, in fragmentation and swelling of myofibers. Despite obvious atrophy, muscle regeneration nevertheless appears to have continued after 30 days in microgravity as evidenced by thin and short newly formed myofibers. Many of them however showed evidence of apoptotic, TUNEL positive cells and myofibrils degradation, suggesting long-term unloading in microgravity affects late stages of myofiber differentiation. Ground asynchronous and vivarium control animals showed normal, well-developed tissue structure with sufficient blood and nerve supply and evidence of regenerative formation of new myofibers free of apoptotic nuclei. Myonuclei stress response in spaceflight animals was detected by positive nuclear immunolocalization of c-jun and c-myc proteins. Regenerative activity of satellite cells in muscles is detected in mice of all animal groups, by pax7, MyoD, and myogenin immunostaining and myogenin PCR analysis. In summary, long-term spaceflight in microgravity causes significant atrophy and degeneration of the femoral Quadriceps muscle group, and it may interfere with muscle regenerative processes by inducing apoptosis in newly-formed myofibrils during their differentiation phase.
    Keywords: Life Sciences (General)
    Type: ARC-E-DAA-TN18686 , Life Sciences in Space Research (ISSN 2214-5524) (e-ISSN 2214-5532); 16; 18-25
    Format: application/pdf
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  • 78
    Publication Date: 2021-05-19
    Description: Over the last decade eutrophication of freshwater artificial reservoirs in Cuba occurred in parallel to oligotrophication of estuarine and coastal waters. These two processes influenced both freshwater and marine fisheries. A dramatic shift in species composition in freshwater fisheries has occurred, from dominance by cichlids (tilapia) to dominance by cyprinids. The high fishery yield from some reservoirs, and shift in species composition, seems related to progressive eutrophication of reservoirs by nutrient subsidies from different anthropogenic activities; particular those related to the size of urban areas within their watersheds. On the other hand, marine landings of estuarine-dependent species declined more significantly than for other groups associated with seagrass beds–coral reefs and oceanic waters. The ratio between catches of estuarine-dependent species and those associated with seagrass beds and coral reefs, decreased significantly over the last 20 years. The decrease in landings was more evident in typical estuarine species, such as shrimps (Litopenaeus schmitti and Farfantopenaeus notialis), mangrove oyster (Crassostrea rhizophorae) and mullets (Mugil spp.). River damming increased during the same period and is significantly correlated with these decreases. It is hypothesized that two different processes acted synergistically, leading to dramatic decreases of several orders of magnitude, in the catches of estuarine species over the last decade: the trapping of nutrients and sediments by river damming, and a drastic reduction in nutrient inputs from land-based sources due to reduced fertilizer use. These are postulated to have affected not only estuarine resources, but also the whole coastal ecosystem.
    Description: Durante la última década, la eutrofización de los embalses artificiales de agua dulce en Cuba se produjo en paralelo a oligotrophication de estuarios y costeras aguas. Estos dos procesos de influencia de agua dulce y la pesca marina. Un cambio dramático en la composición de las especies en la pesca de agua dulce se ha producido, de la dominación de los cíclidos (tilapia) a la dominación de ciprínidos. La alta producción de la pesca de algunos embalses, y el cambio en las especies composición, parece relacionada con la progresiva eutrofización de los embalses de las subvenciones de nutrientes de diferentes actividades antropogénicas, especialmente las relacionado con el tamaño de las zonas urbanas dentro de sus cuencas. Por otro lado, los desembarques de especies marinas de estuario-dependientes disminuyeron significativamente más que para otros grupos asociados con algas marinas Los arrecifes de coral-camas y las aguas oceánicas. La relación entre las capturas de las especies que dependen de los estuarios y las asociadas a las praderas marinas y Los arrecifes de coral, se redujo significativamente en los últimos 20 años. La disminución en los desembarques fue más evidente en especies típicas de estuarios, tales como camarones (Litopenaeus schmitti y Farfantopenaeus notialis), ostra de mangle (Crassostrea rhizophorae) y la lisa (Mugil spp.). El represamiento del Río aumentó durante el mismo período y se correlaciona significativamente con estas disminuciones. Se planteó la hipótesis de que dos procesos diferentes actúan de forma sinérgica, que conduce a una disminución drástica de varios órdenes de magnitud, en las capturas de las especies de estuario en la última década: la captura de nutrientes y sedimentos por represas río, y una reducción drástica de los aportes de nutrientes de fuentes terrestres, debido a la reducción del uso de fertilizantes. Se postula que han afectado no sólo a los recursos estuarinos, sino también todo el ecosistema costero.
    Description: Published
    Description: ríos
    Description: impactos antropogénicos
    Keywords: river ; Fisheries ; Eutrophication
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 79
    Publication Date: 2021-05-19
    Description: Se muestra la evolución de las capturas de las sierras y pintadas, se describen las 3 maneras en que las especies pueden quedar atrapadas en la redes de enmalles, así como también los tipos de redes (deriva, media agua y de fondo).
    Description: Published
    Description: Scomberomorus regalis
    Description: Scomberomorus maculatus
    Description: Scomberomorus cavalla
    Description: spanish mackerel
    Description: king mackerel
    Description: painted mackerel
    Description: enmalle
    Description: arte de pesca
    Keywords: Fisheries ; Artisanal fishing
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 80
    Publication Date: 2021-05-19
    Description: Published
    Description: Mugil curema
    Description: Mugil liza
    Description: Mugil cephalus
    Description: Gerres cinereus
    Description: patao
    Description: coastal lagoons
    Keywords: Mullet fisheries ; Fisheries
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 81
    Publication Date: 2021-05-19
    Description: Ce travail représente une contribution à l'étude de l'activité de pêche hauturière dans le gouvernorat de Médenine, particulièrement, au niveau du port de Zarzis ; durant la période (juin 2005-juin 2006). Il consiste à réaliser, grâce à des enquêtes menées au port, deux types d'analyses : une analyse quantitative afin de déterminer les débarquements saisonniers moyens des espèces les plus débarquées par la pêche hauturière et une analyse qualitative pour établir les structures démographiques des espèces les plus exploitées dans la région. Les résultats obtenus ont montré que, durant la période d'étude, les principales espèces débarquées par la pêche hauturière sont la sardinelle, le maquereau, le saurel, le rouget blanc, le pageot, la thonine, la seiche et la crevette royale. En examinant les débarquements saisonniers moyens des espèces les plus débarquées par type de pêche, nous avons remarqué que deux ou trois espèces dominent pour chaque saison. De plus, l'analyse des structures démographique des apports a montré que la majorité des individus débarqués sont matures, à l’exception du maquereau dont les captures sont constituées d’individus immatures, surtout pendant les saisons d’été et d’automne.
    Description: This work represents a contribution to the study of the offshore fishing activity in the governorate of Medenine, particularly at the port of Zarzis, during the period (june 2005-june 2006. It is to realize, through surveys conducted at the port, two types of analyzes: a quantitative analysis to determine the average seasonal landings of most landed species by fishing offshore units and qualitative analysis to determine the demographic structure of the most exploited species in the region. The obtained results showed that during the period of study, the main landed species by offshore fishing are sardinella, mackerel, horse mackerel, white mullet, sea bream, skipjack tuna, cuttlefish and the caramote prawn. By examining the average seasonal landings of the most landed species, we noticed that, for each season, two or three species dominate. Furthermore, analysis of demographic structures of the landings showed that the majority of landed individuals are mature, with the exception of mackerel catches which are composed of immature individuals, especially during the seasons of summer and autumn.
    Description: Published
    Description: analyse qualitative
    Description: pêche hauturière
    Keywords: Quality analysis ; Offshore ; Fisheries
    Repository Name: AquaDocs
    Type: Proceedings Paper , Refereed , Meeting abstract
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  • 82
    Publication Date: 2021-05-19
    Description: Se muestran las capturas históricas de la biajaiba (1935-2000), la evolución de las capturas del pargo (una de las especies de mayor interés económico en Cuba). Se evalúa la utilización de éstas artes de pesca en especies comerciales y su implicación con daños al medio ambiente. Se tiene en cuenta que los pargos criollos, la biajaiba y el caballerote representan el 20 % de las capturas.
    Description: Published
    Description: pesquería artesanal
    Description: cubera
    Description: cherna criolla
    Description: pesca del alto
    Description: pargo
    Description: rabirrubia
    Description: caballerote
    Description: biajaiba
    Keywords: Fisheries ; Artisanal fishing
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 83
    Publication Date: 2021-05-19
    Description: Un análisis de los datos arqueológicos e históricos sobre la pesca en Cuba muestra que el impacto de la población aborigen sobre los recursos pesqueros no fue significativo debido a su baja densidad poblacional y a las tecnologías de pesca. El interés por el oro, el tamaño de la población, las limitaciones tecnológicas para la pesca y preservación de las capturas así como las preferencias dietéticas de los españoles todas indican que la pesca tampoco fue una actividad económica importante durante los tres siglos que siguieron a la llegada de Colón. Las preferencias por la carne de res y el bacalao salado y la baja tasa de crecimiento poblacional durante el período colonial determinaron que la mayor parte de los recursos pesqueros, con la excepción del manatí y las tortugas marinas, permanecieran prácticamente inexplorados durante varios siglos después de la Conquista. Los datos estadísticos e históricos revelan que la pesca experimentó un rápido crecimiento desde 1950 y este patrón parece ser una característica común en el Caribe así como en otras partes del mundo. Las presiones sobre la vida marina fueron más evidentes en la segunda mitad del siglo xx cuando el crecimiento poblacional, las mejoras tecnológicas y las demandas del mercado aceleraron el sector pesquero así como la urbanización en la mayoría de los países del caribe.
    Description: Published
    Keywords: Integrated management ; Fisheries
    Repository Name: AquaDocs
    Type: Proceedings Paper , Refereed , Article
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  • 84
    Publication Date: 2021-05-19
    Description: A yield per recruit model has been used to compare the effects of mesh size increment on the yields and revenues of the fisheries of Cameroon under two different cases. Case 1 assumes the commercial fishery to move from the exploitation of three age-groups to two age-groups with no interactions with the artisanal fishery, whereas Case 2 takes into account these interactions. The difference in the percentage increase of yield per recruit between case 1 and case 2 is 61% at current fishing (46% and 18% yield per recruit increment in cases 1 and 2 respectively). The usually accepted long-term yield per recruit increment with increase of age at first capture (with a single non-interacting fisheries) is, in this case, cancelled out. However, the revenues increase by 72% and 63% in cases 1 and 2 respectively. Therefore the economic approach, compared with purely biological analyses, is more convincing. In general, as fisheries always interact, a single-fishery management approach should not be the rule as it is at present; management strategies should consider interactions between different fisheries and be based on their economic performances and not, as said earlier, on purely biological considerations. This is because a biological approach to fisheries management will, at best, be modified by economic factors, or, at worst, be ignored totally in favour of economic policies.
    Description: Published
    Keywords: Biology ; Fisheries ; Biological interaction ; Sciaenidae ; Fisheries development ; Fishery management ; Yield/recruit ; Commercial fisheries ; Artisanal fishing
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 85
    Publication Date: 2021-05-19
    Description: La pêche au Sénégal joue un rôle économique et social très important par une contribution globale de près de 11% du PIB primaire et 2,3% du PIB total. Elle occupe de façon directe et indirecte environ 600 000 personnes, soit près de 17% de la population active selon la DP M. Cependant ces dernières décennies, les études ont montré que le secteur traverse une crise aiguë se traduisant par une raréfaction croissante des ressources, notamment les espèces nobles qui ont une forte valeur commerciale. Cette crise est due entre autres à une surexploitation de ces ressources qui est le résultat d’un manque de maîtrise de l’effort de pêche surtout pour les pêcheries artisanales (80% des débarquements et plus de 60% des approvisionnements des industries de la place) au regard du volume de ressources disponibles. Ce manque de maîtrise est dû essentiellement par un défaut du suivi contrôle et surveillance au niveau de la pêche artisanale par l’Etat, qui n’a pas les moyens humains et matériels pour appliquer des mesures de gestion, qui pour la plupart ne sont pas adaptées aux réalités des acteurs à la base. L’objet de cette étude est d’analyser une nouvelle approche axée sur la surveillance participative basée sur l’émergence d’initiatives locales de cogestion au niveau des quatre (04) sites du programme GIRMaC que sont ; Ouakam, Ngaparou, Foundiougne et Bétenty. Il ressort de cette étude que : • Le secteur de la pêche traverse une crise aiguë due principalement selon les acteurs à un défaut de suivi, contrôle et surveillance tel que appliqué actuellement ; • L’approche de cogestion avec des bases juridiques cohérentes, comme le propose le programme GIRMaC peut être un moyen viable pour solutionner à la crise que traverse la pêche ; • D’une part le système Suivi Contrôle et Surveillance coûte cher, d’autre part que ces coûts sont fortement amoindris lorsque les acteurs participent à l’effort de surveillance, par une démarche de surveillance participative en cogestion.
    Description: Président : Omar Thiom THIAW, Professeur UCAD Membres : • Niokhor DIOUF, Chercheur IUPA. • Luis Tito Morais, Chercheur IRD Dakar. • Djiby THIAM, Chercheur GIRMac
    Description: Published
    Description: surveillance; contrôle; cogestion; effort de pêche; pêcherie; surexploitation; biodiversité; pêche artisanale
    Keywords: Ocean surveillance ; Ecosystem management ; Fishing effort ; Overexploitation ; Biodiversity ; Artisanal fishing ; Fisheries
    Repository Name: AquaDocs
    Type: Theses and Dissertations , Master thesis
    Format: 87
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  • 86
    Publication Date: 2021-05-19
    Description: Published
    Description: espéces pélagiques
    Description: sardine
    Description: pêche industrielle
    Keywords: Fisheries ; Pelagic fisheries ; Industrial fish ; Fish products ; Fishing industry ; Fishing fleet
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 87
    Publication Date: 2021-05-19
    Description: Unpublished
    Description: conservation
    Description: chaine froide
    Description: poisson
    Keywords: Food conservation ; Fisheries ; Fish conversion ; Conservation (fishery products) ; Glass ; Fisheries
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 88
    Publication Date: 2021-05-19
    Description: Published
    Description: Pêche
    Description: Senneur
    Keywords: Seining ; Fisheries ; Fisheries ; Seiners
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 89
    Publication Date: 2021-05-19
    Description: ACDI;CRDI; République Française, Ministère des Affaires Etrangéres; CDE; Enda Graf Sahel;
    Description: Conseil Info MPEA, Projer d'Accés à l'information et au Conseil pour les microet petites entreprises agroalimentaires; Projet d'Appui aux Opérateurs/trices de l'Agroalimentaires (PAOA); Cintech Agroalimentaire; Groupe de Recherche et d'Echanges Technologiques (GRET); Enda GRaf Sahel; SNC-LAVALIN International
    Description: Published
    Description: transformation; transformation artisanale; échantillonage; collecte de données; commercialisation
    Keywords: Processing fishery products ; Biological sampling ; Data collections ; Marketing and distribution ; Fisheries
    Repository Name: AquaDocs
    Type: Theses and Dissertations , Master thesis
    Format: 41
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  • 90
    Publication Date: 2021-05-19
    Description: Français - Les traits essentiels de la côte sénégalaise, repères historiques de la pêche, communautés de pêcheurs, caractéristiques des flottes et modes d’accès à la ressource et d’interaction sont analysés. Les caractéristiques, tendances décroissantes de l’abondance,niveaux d’exploitation, mesures d’aménagement, relations interspécifiques, changements potentiels et sources de variation des stocks démersaux côtiers sont précisés. Les notions de tactiques et stratégies de pêche sont passées en revue, conceptualisées puis étudiées.Le modèle «Dynamique Conjointe Exploitation Ressource» utilisé repose sur la définition de 4 typologies. L’utilisation de méthodes de classification et de connaissances d’experts conduit à considérer 32 stocks, 31 strates, 82 tactiques et 25 flottilles artisanales et chalutières. Les typologies sont articulées avec la prise en compte de paramètres relatifs à l’économie, aux stocks, flottilles et règles de décisions. L’ajustement est satisfaisant en partie. Toutefois, le modèle garde son importance en matière de prospective et d’objectifs à atteindre via la définition de variables de contrôle. Notre travail peut être considéré comme une esquisse invitant à un groupe de travail élargi aux experts des institutions halieutiques nationales voire, sous-régionales. Des propositions sont faites sur les bases de données, les licences de pêche et l’étude des tactiques et stratégies à mener suivant des pas de temps raisonnables pour tenir compte du grand dynamisme des pêcheries. Dès lors qu'il s’agit de caractériser l'impact de la pêche sur l'écosystème, ces travaux sont des éléments essentiels des recherches à faire sur la dynamique des écosystèmes exploités. English - The essential features of the Senegalese coast, fisheries history, fishermen's communities, artisanal and trawling fleet’s characteristics and their way of accessing to the resource and interactions are described. The characteristics, evolutionary tendencies of abundance, level of exploitation, measures of fisheries management, interspecific relationship, potential evolutionary changes and sources of variation of coastal demersal stocks are specified. Tactics and strategies are reviewed, conceptualized and studied. We used the model "Dynamique Conjointe Exploitation Ressource" which lies on 32 stocks, 31 strata, 82 tactics, 25 fleets and several other parameters dealing with stocks, fleets, economy and making decision rules. The adjustment is partially satisfactory but the model is of great interest for prospective studies and objectives to reach once defined variables of control. Our work is an outline calling for an enlarged working group implying national or sub regional fisheries institutional experts. Tactics and strategies studies, re-actualized in artisanal fisheries, are performed for the first time in industrial fisheries. The typological approach is simple, pertinent, efficacy and fast. Propositions are made relatively to databases, fishing licenses and tactics and strategies studies that should be implemented according to a reasonable time steps in order to take into account the big dynamism of the Senegalese fisheries. As it is intended to characterize fisheries impact on ecosystem, these works are one of essential research elements on the dynamic of exploited ecosystems.
    Description: Published
    Description: pêcherie; démersale côtière; modélisation; stocks; écosystème; communauté de pêcheurs; eaux continentales; eaux marines; saison hydrologique; pêcheur; flottille; crevettier; capture; aménagement des pêcheries; pêche chalutière; pêche artisanale; licence de pêche; permis de pêche
    Keywords: Fisheries ; Demersal fisheries ; Modelling ; Stocks ; Ecosystems ; Community fishing ; Inland waters ; Marine water ; Hydrologic cycle ; Fishermen ; Fishing fleet ; Shrimp fisheries ; Capture fisheries ; Fisheries management ; Trawling ; Artisanal fishing ; Fishing licenses
    Repository Name: AquaDocs
    Type: Theses and Dissertations , Bachelor thesis
    Format: 234
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  • 91
    Publication Date: 2021-05-19
    Description: Published
    Description: conservation
    Description: produits
    Description: pêche
    Keywords: Conservation ; Fisheries ; Fish products
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 92
    Publication Date: 2022-05-25
    Description: Author Posting. © Ecological Society of America, 2013. This article is posted here by permission of Ecological Society of America for personal use, not for redistribution. The definitive version was published in Ecological Applications 23 (2013): 959–971, doi:10.1890/12-0447.1.
    Description: The biological benefits of marine reserves have garnered favor in the conservation community, but “no-take” reserve implementation is complicated by the economic interests of fishery stakeholders. There are now a number of studies examining the conditions under which marine reserves can provide both economic and ecological benefits. A potentially important reality of fishing that these studies overlook is that fishing can damage the habitat of the target stock. Here, we construct an equilibrium bioeconomic model that incorporates this habitat damage and show that the designation of marine reserves, coupled with the implementation of a tax on fishing effort, becomes both biologically and economically favorable as habitat sensitivity increases. We also study the effects of varied degrees of spatial control on fisheries management. Together, our results provide further evidence for the potential monetary and biological value of spatial management, and the possibility of a mutually beneficial resolution to the fisherman–conservationist marine reserve designation dilemma.
    Description: M. G. Neubert acknowledges the support of the National Science Foundation (DMS-0532378, OCE-1031256) and a Thomas B. Wheeler Award for Ocean Science and Society. H. V. Moeller acknowledges support from a National Science Foundation Graduate Research Fellowship. This research is based in part on work supported by Award No. USA 00002 made by King Abdullah University of Science and Technology (KAUST).
    Keywords: Bioeconomics ; Destructive fishing practices ; Fisheries ; Habitat damage ; Marine protected areas ; Marine reserves ; Optimal control ; Optimal harvesting ; Spatial management
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 93
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    Ministerio de Medio Ambiente y Medio Rural y Marino | Madrid (Espagne)
    Publication Date: 2021-05-19
    Description: Description du secteur de la pêche Avec la Mauritanie, le Sénégal est de loin le plus grand producteur de produits de pêche de la sous région. Avec 718 km de cote et un territoire maritime de 198km2, 15 745 km2 de superficie fluviale, 2 estuaires, des mangroves et un lac de 350km2, le Sénégal possède tous les atouts des plus grands producteurs de pêche. Le potentiel annuel de captures s’élève à 500 000 tonnes. Le secteur a subi une croissance spectaculaire ces trente dernières années : les débarquements sont passés 50 000 tonnes en 1965 à plus de 450 000 en 1997. Le secteur se compose principalement de deux sous-secteurs : la pêche industrielle et la pêche traditionnelle ou artisanale. Cette dernière, vielle de plusieurs siècles a su s’adapter et se moderniser au cours des dernières décennies. La flotte de pêche artisanale est dynamique et diversifiée ; selon les estimations, en 2005 il y avait plus de 13 000 pirogues. La pêche artisanale se concentre dans sept régions maritimes et fluviales du pays: Dakar, Thiès, Saint Louis, Fatick, Zinguinchor, Louga et Kaolack. Le port de Kayar dans la région de Thiès correspond à la principale zone de débarquement de la pêche artisanale avec environ 70% des débarquements. Dans le secteur industriel, les pêcheurs travaillent à bord d’une flottille de petits sardiniers, de 143 chalutiers destinés à la pêche d’espèces demersales, de 3 sardiniers et de 42 thoniers. Alors que le Sénégal domine largement le sous-secteur demersale avec 70% des chalutiers opérants, le pays ne possède que 6 thoniers contre 38 étrangers, dont une partie seulement des captures est débarquée au Sénégal. La pêche industrielle est concentrée autour de la capitale dans le Port autonome de Dakar et des ports secondaires de Saint Louis, Kaolack et Ziguinchor. Aujourd’hui, le secteur de la pêche sénégalaise est largement dominé par la pêche artisanale, qui constituait 90% des cargaisons totales des produits de la mer en 2006 avec 350 000 tonnes. Plus de 60% des produits de la pêche artisanale sont destinés à l’exportation et la transformation. En 2006, le secteur dans son ensemble représentait 9% du PIB du secteur primaire, 1.5% du PIB total, et 1/3 des devises étrangères (250 millions de dollars par an). Plus importante que le poids économique du secteur, est son poids social : la pêche génère indirectement 600 000 emplois, dont 67% au sein du secteur artisanal. En outre, les produits de la pêche comblent les besoins en protéines animales de la population sénégalaise à hauteur de 75%1. La pêche a connu une expansion spectaculaire au cours des dernières décennies grâce à l’impulsion de politiques publiques de développement basées sur une logique sectorielle productiviste. Une flotte nationale artisanale et industrielle a été progressivement mise en place ainsi que de fortes capacités de transformation industrielle. Cet engagement de l’Etat a ensuite connu un net ralentissement du fait, entre autre, des politiques d’ajustement structurel. Le renchérissement des coûts de production subséquents à la dévaluation du franc CFA en 1994 a lourdement affecté les pêcheurs. Parallèlement, avec la forte demande en produits halieutiques et en l’absence de politiques adéquates et cohérentes de gestion durable des ressources exploitables, une situation de surexploitation des ressources halieutiques s’est installée. En conséquence de la raréfaction des ressources halieutiques, la pêche sénégalaise traverse une crise sans précédent, dont les effets commencent à se faire sentir à tous les niveaux : appauvrissement des communautés de pêcheurs, menace de l’approvisionnement en poisson des populations, baisse des captures à haute valeur ajoutée, baisse des exportations, baisse de la rentabilité et des revenus des unités de pêche. Par ailleurs, de plus en plus d’établissements de transformation industrielle ferment (23 entreprises entre 1999 et 2006).
    Description: Ministerio de Medio Ambiente Y Medio Rural y Marino
    Description: Pablo Manuel Xandri Royo CTP Proyecto (INT/07/16M/SPA) Este trabajo se ha realizado en el marco del Convenio de Colaboración entre el actual Ministerio de Medio Ambiente y Medio Rural y Marino de España y la Organización Internacional del Trabajo de 28 de diciembre de 2007
    Description: Published
    Description: environnement; socio-économie; pêche; capture; débarquement; engin de pêche; femme; pêcheur; convention internationale; réglementation
    Keywords: Fisheries ; Marine environment ; Socioeconomic aspects ; Capture fisheries ; Landing statistics ; Fishing gear ; Women ; Fishermen ; Conventions ; Fishery regulations
    Repository Name: AquaDocs
    Type: Report , Non-Refereed
    Format: 208
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  • 94
    Publication Date: 2021-05-19
    Description: Programme KURUKAN FUGAN (Union Européenne/Gouvernement du Sénégal/EndaGraf/GRET)
    Description: Published
    Description: politique de pêche; environnement marin; socio-économie; co-gestion; aquaculture; pêche artisanale; pêche industrielle; pêcherie; ONG; AMP; ressource halieutique; organisations professionnelles
    Keywords: Marine environment ; Socioeconomic aspects ; Fishery management ; Aquaculture ; Artisanal fishing ; Fisheries ; Industrial fish ; Fishery resources ; Fishing policy ; Protected areas ; Professionals
    Repository Name: AquaDocs
    Type: Book , Non-Refereed , Article
    Format: 26
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  • 95
    Publication Date: 2021-09-18
    Description: En la época colonial algunas especies de peces cubanos retienen sus nombres por los cuales eran conocidos por los aborígenes: biajaca, biajaiba, cojinúa, jiguagua, cibí. Aún hoy se designan de igual forma. En esta época aparece la primera obra científica impresa en Cuba, los primeros pueblos pescadores y los primeros científicos.
    Description: Published
    Keywords: Fisheries
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 96
    Publication Date: 2021-09-18
    Description: Published
    Description: especies nativas
    Description: pesca
    Keywords: Fisheries ; Indigenous species ; Indigenous fishing
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 97
    Publication Date: 2021-09-18
    Description: Published
    Keywords: Fisheries
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 98
    Publication Date: 2021-09-18
    Description: Recuento en forma breve de las pesquerías de estas especies durante varios siglos, en los cuales los sabios Parra y Felipe Poey ofrecen descripciones. Precios, lugares de captura y pescadores son los protagonistas.
    Description: Published
    Description: Emperador-Xiphias gladius
    Description: pez espada
    Description: castero-Makaira nigricans
    Description: aguja blanca-Tetrapturus albidus
    Keywords: Fisheries ; Fishes
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 99
    Publication Date: 2021-09-18
    Description: Causas del descenso en las pesquerías de estas especies a partir de 1975. Se describen las técnicas y artes de pesca empleadas y se abunda en la complejidad de las capturas y las características de los palangres empleados para cada especie. Una comparación es establecida además con países del área. De forma muy didáctica e instructiva.
    Description: Published
    Description: castero-Makaira nigricans
    Description: Emperador-Xiphias gladius
    Description: aguja blanca-Tetrapturus albidus
    Keywords: Fisheries ; Fishes
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 100
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    Unknown
    Nature Publishing Group (NPG)
    Publication Date: 2013-07-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Walker, Cameron -- England -- Nature. 2013 Jul 4;499(7456):115-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23841144" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Authorship ; Biodiversity ; Congresses as Topic ; *Cooperative Behavior ; Ecology/education ; *Education, Graduate ; Fisheries ; *Group Processes ; International Cooperation ; Multicenter Studies as Topic ; Research Personnel/*education ; Research Report ; Statistics as Topic
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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