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  • 1
    Electronic Resource
    Electronic Resource
    Unstable genes affecting chloroplast development in soybean (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 10 (1989), S. 532-541 
    Details
    ISSN: 0192-253X
    Keywords: Variegation ; Transposable elements ; Thylakoid membranes ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Y18 is a nuclear gene of soybean (Glycine max) necessary for normal chloroplast development. An unstable allele (Y18-m) of the Y18 gene has been previously characterized genetically [Peterson and Weber: Theor Appl Genet 39:156-162, 1969.] Plants homozygous for the unstable allele produce leaves that exhibit a variegated pattern of green and yellow leaf sectors, indicating somatic mutability events. Germinal instability is detected by the recovery of either pure breeding dominant green (rare) or pure breeding recessive yellow (frequent) plants from the mutable stock. In contrast to most unstable genes identified in other plant systems, the Y18-m mutation is from the dominant green state to the recessive yellow state, producing a pattern of “reverse variegation.” Current work has focused on further characterization of this mutation at the whole plant level as well as at the biochemical level. These results include observations on the cell- and tissue-type specificity of the mutation, stability of the recessive yellow mutation, and a biochemical analysis of mutant and normal thylakoid membranes to identify the specific polypeptides affected by the y18 mutation. Several polypeptides of the thylakoid membranes are missing, and many, including the major light harvesting complex (LHCP) polypeptides, are reduced. Messenger RNAs for LHCPII were also reduced to a greater extent than other leaf transcripts in the yellow sectors of variegated plants. A comparison of Y18-m to other soybean mutable genes and transposable element insertions is made.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020100612
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  • 2
    Electronic Resource
    Electronic Resource
    Masthead (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110101
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  • 3
    Electronic Resource
    Electronic Resource
    Introduction: The expression and function of specific genes in early embryonic development (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 1-1 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110102
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  • 4
    Electronic Resource
    Electronic Resource
    Developmental expression of regionally specific cell surface antigens in the Xenopus gastrula (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 110-122 
    Details
    ISSN: 0192-253X
    Keywords: Embryonic cell surface ; glycoconjugates ; monoclonal antibodies ; developmental expression of glycoconjugates ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Molecular markers for specific cell lineages would be useful in studies of cellular differentiation. To isolate such markers monoclonal antibodies (MoABs) were raised against plasma membranes isolated from gastrulating Xenopus embryos. Those antibodies that recognized subsets of cells within the embryo were selected by indirect immunofluorescence. The analysis of eight such MoAbs is presented. Western blot analysis showed that all but one MoAb recognized a complex pattern of glycoconjugates associated with glycoproteins. All the antigens recognized by the MoAbs were maternal in origin and displayed similar spatial patterns of pregastrular expression. This pattern of immunoreactivity at the apical surface was inherited passively during cleavage by the resulting superficial blastomeres suggesting that ectodermal specific markers of maternal origin are pre-localized to the cortical ooplasm in mature oocytes. We suggest that these maternal components may be specific glycosyl transferases. Three different patterns of expression were observed during gastrulation as exemplified by MoAbs 1F10C1, 3A4D1, and 6F10B6. MoAb 6F10B6 was specific for both neural and non-neural epithelium. MoAb 3A4D1 was specific for non-neural epidermis. MoAb 1F10C1 appeared to recognize a protein epitope on an extracellular component expressed by the superificial and involuting epithelial cells. The pattern of expression for the 1F10C1 antigen suggests that it may play a role in facilitating the movement of the involuting cells during gastrulation.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110112
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic alteration of normal aging processes is responsible for extended longevity in Drosophila (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 141-148 
    Details
    ISSN: 0192-253X
    Keywords: Aging ; longevity ; genes and aging ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The first step in a genetic analysis of aging is to identify and characterize the genetic mutants and their controls that will be used. Such mutants or strains are initially identified by their effect on the life span. Yet many genetic interventions are known to have some effect on the life span without necessarily affecting the aging process. It is therefore necessary to prove that one is actually dealing with an aging mutant before one draws strong inferences from the data. Casarett's rules provide an operational test for doing so, relying as they do on the comparison of aging biomarkers in the experimental and reference strains. We show that our previously described genetically based long-lived NDC-L strain and its normal-lived NDC-R control strain differ only in the chronological age of expression of two behavioral and three physiological functional age biomarkers. They do not differ in the sequence or the physiological age of expression of these biomarkers. These two strains comply with the Casarett rules and thereby comprise a valid tool with which to conduct a comparative genetic analysis of aging. The implications of the available data are discussed, including the possibility that aging in these strains of Drosophila melanogaster may be the result of a multiphasic developmental process.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110204
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  • 6
    Electronic Resource
    Electronic Resource
    Butyrate induces expression of transfected human fetal and endogenous mouse embryonic globin genes in GM 979 erythroleukemia cells (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 168-174 
    Details
    ISSN: 0192-253X
    Keywords: Human fetal globin gene ; hemoglobin switching ; mouse erythroleukemia cells ; erythroid differentiation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have analyzed the expression of endogenous murine genes and of transfected human fetal Aγ globin gene in GM 979, a mouse erythroleukemia line which produces adult as well as embryonic globins. Optimal induction of the endogenous murine adult globin genes was obtained with DMSO or HMBA while the ∊y and βh1 embryonic genes were preferentially induced by butyrate. Similarly, the transferred human Aγ globin gene was preferentially induced by butyrate. These results as well as previous observations in vivo or in erythroid cell cultures suggest that butyrate preferentially induces the expression of fetal globin genes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110207
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  • 7
    Electronic Resource
    Electronic Resource
    Masthead (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110301
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  • 8
    Electronic Resource
    Electronic Resource
    Regulation of nuclear genes encoding chloroplast proteins in transgenic plants (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 197-204 
    Details
    ISSN: 0192-253X
    Keywords: Light-regulated genes ; transgenic plants ; enhancer ; silencer ; regulatory elements ; trans-acting factors ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Transgenic plants have been particularly useful in studying nuclear genes encoding for photosynthetic functions. The expression of these genes and their chimeric constructs in transgenic plants faithfully mimics their natural counterparts. The use of sensitive chimeric reporter genes has enabled localizing the activity of genes encoding photosynthetic proteins to individual cells. Cab and rbcS transgenes have been shown to retain sensitivity to light quality, which is modulated by phytochrome. Conditional light activation under the influence of a circadian rhythm has been shown for Cab transgenes. Transgenic plants containing truncated promoters have helped delineate cis-regulatory positive and negative elements involved in light-mediated transcriptional induction and tissue specificity.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110305
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  • 9
    Electronic Resource
    Electronic Resource
    Masthead (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110401
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  • 10
    Electronic Resource
    Electronic Resource
    Bacterial and firefly luciferase genes in transgenic plants: Advantages and disadvantages of a reporter gene (1990)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 224-232 
    Details
    ISSN: 0192-253X
    Keywords: lux ; luc reporter genes ; light emission ; gene expression ; single photon imaging in vivo ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Genes encoding light-emitting luciferase were recently isolated from luminous marine bacteria and fireflies. Expression of luciferase genes in diverse organisms is a unique way for studying gene expression by simple and sensitive measurement of light. Recent advances in application of luciferase reporter genes are reviewed and documented by examples of in vivo visualization of their expression in transgenic plants.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020110308
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