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  • Articles  (6,077)
  • Oxford University Press
  • Electrical Engineering, Measurement and Control Technology  (4,934)
  • Geography  (1,143)
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  • Articles  (6,077)
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  • 1
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    Cover Page (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Print ISSN: 0022-0744
    Electronic ISSN: 1477-9986
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Published by Oxford University Press on behalf of The Japanese Electron Microscopy Society.
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  • 2
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    Subscription Page (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Print ISSN: 0022-0744
    Electronic ISSN: 1477-9986
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 3
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    Recent advancements in structured-illumination microscopy toward live-cell imaging (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Description: Fluorescence microscopy allows us to observe fluorescently labeled molecules in diverse biological processes and organelle structures within living cells. However, the diffraction limit restricts its spatial resolution to about half of its wavelength, limiting the capability of biological observation at the molecular level. Structured-illumination microscopy (SIM), a type of super-resolution microscopy, doubles the spatial resolution in all three dimensions by illuminating the sample with a patterned excitation light, followed by computer reconstruction. SIM uses a relatively low illumination power compared with other methods of super-resolution microscopy and is easily available for multicolor imaging. SIM has great potential for meeting the requirements of live-cell imaging. Recent developments in diverse types of SIM have achieved higher spatial (~50 nm lateral) and temporal (~100 Hz) resolutions. Here, we review recent advancements in SIM and discuss its application in noninvasive live-cell imaging.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 4
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    STED microscopy--super-resolution bio-imaging utilizing a stimulated emission depletion (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Description: One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we first describe the principles of STED microscopy and then describe the features of our newly developed two-photon excitation STED microscopy. On the basis of our recent results and those of other researchers, we conclude by discussing future research and new technologies in this field.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 5
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    For Microscopy special feature on 'super resolution microscopy' (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Print ISSN: 0022-0744
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 6
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    Ultrathin sectioning with DUV-pulsed laser ablation: development of a laser ablation nano tome (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Description: The electrically automated ultrathin sectioning apparatus, which has been developed in recent years, can produce consecutive ultrathin sections with a diamond knife and a gallium ion beam. These newly developed apparatuses, however, have several shortcomings, such as the limited block cutting area, thermal damage to the sample by the focused ion beam and a sample electronic charge. To overcome these faults and for easier scanning electron microscopy three-dimensional fine structural reconstruction, we have developed a new cutting method using a deep ultraviolet laser, which we have named the ‘LANTome (Light Ablation Nanotome)’. Using this method, we confirmed the widening of sectioning areas, shortening of the sectioning time, automatic smoothing of rough surfaces, no sample electronic charge and minimal heat effects on the sample tissue, such as thermal denaturation.
    Print ISSN: 0022-0744
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 7
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    A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Description: Recent advances in nanoscopy, which breaks the diffraction barrier and can visualize structures smaller than the diffraction limit in cells, have encouraged biologists to investigate cellular processes at molecular resolution. Since nanoscopy depends not only on special optics but also on ‘smart’ photophysical properties of photocontrollable fluorescent probes, including photoactivatability, photoswitchability and repeated blinking, it is important for biologists to understand the advantages and disadvantages of fluorescent probes and to choose appropriate ones for their specific requirements. Here, we summarize the characteristics of currently available fluorescent probes based on both proteins and synthetic compounds applicable to nanoscopy and provide a guideline for selecting optimal probes for specific applications.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 8
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    Aberrations and adaptive optics in super-resolution microscopy (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Description: As one of the most powerful tools in the biological investigation of cellular structures and dynamic processes, fluorescence microscopy has undergone extraordinary developments in the past decades. The advent of super-resolution techniques has enabled fluorescence microscopy – or rather nanoscopy – to achieve nanoscale resolution in living specimens and unravelled the interior of cells with unprecedented detail. The methods employed in this expanding field of microscopy, however, are especially prone to the detrimental effects of optical aberrations. In this review, we discuss how super-resolution microscopy techniques based upon single-molecule switching, stimulated emission depletion and structured illumination each suffer from aberrations in different ways that are dependent upon intrinsic technical aspects. We discuss the use of adaptive optics as an effective means to overcome this problem.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 9
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    Why did the Portuguese economy stop converging with the OECD? Institutions, politics and innovation (2015)
    Oxford University Press
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    Publication Date: 2015-08-22
    Description: Underlying the crisis affecting peripheral European countries is their structural, long-term loss of competitiveness (Hadjimichalis, 2011, European Urban and Regional Studies , 18: 254–274). This article will focus on the Portuguese case and discuss the institutional constraints that hindered its economy from transitioning towards the production of higher-value added goods and services. It will discuss institutions as the product of a political process laden with power asymmetries and argue that the dominance of a relatively small community at the heart of economic and political life in Portugal has conditioned the development of the economy as a whole. Using this framework, this article will then contribute to the literatures on innovation and technological modernisation and argue that alongside a technical process of catching up there is a political process that can enable or constrain development.
    Keywords: B52 - Institutional ; Evolutionary, O33 - Technological Change: Choices and Consequences ; Diffusion Processes, P16 - Political Economy
    Print ISSN: 1468-2702
    Electronic ISSN: 1468-2710
    Topics: Geography , Economics
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  • 10
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    The geography of financial inclusion across Europe during the global crisis (2015)
    Oxford University Press
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    Publication Date: 2015-08-22
    Description: This article identifies the main determinants of financial inclusion, defined as the probability of using both banking and credit services, across 18 Eastern European economies and 5 Western European ‘comparator’ countries. We elicit demographic and socio-economic information on 25,000 European households from the second round of the Life in Transition Survey undertaken during the 2008–2010 global crisis; the survey also includes several questions on households’ financial decisions collecting data at the regional and local level. Our results show that households hit by negative job or income shocks and without any asset to pledge are less likely to be financially included, especially in Eastern Europe. The individual likelihood of financial inclusion is also affected by the average use of financial services at the local level suggesting the presence of a financial multiplier effect. These results provide useful information for mapping financial inclusion across Europe during the crisis, which in turn can inform policy action at the local level.
    Keywords: D14 - Personal Finance, G21 - Banks ; Other Depository Institutions ; Micro Finance Institutions ; Mortgages
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    Topics: Geography , Economics
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  • 11
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    Analysis of the complexity of the sperm acrosomal membrane by super-resolution stimulated emission depletion microscopy compared with transmission electron microscopy (2015)
    Oxford University Press
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    Publication Date: 2015-08-09
    Description: The acrosome is a Golgi-derived sperm cell organelle enclosed by a continuous acrosomal membrane. The acrosomal membrane complexes with surrounding matrices containing molecules necessary for fertilization; however, the complex of acrosomal membrane and associating matrices (CAMAM) has not been visualized in detail under living conditions. Here, we analyzed the CAMAM at the nanometer level using super-resolution stimulated emission depletion (STED) fluorescence microscopy and equatorin-enhanced green fluorescent protein transgenic mice. The STED images were compared with the corresponding images taken by immunoelectron microscopy. Consequently, the substructure of CAMAM could be differentiated at nanometer-scale resolution by STED microscopy without the need for sectioning. The information obtained in this study will be beneficial not only for understanding the molecular mechanism of fertilization but also for cell imaging under living conditions.
    Print ISSN: 0022-0744
    Electronic ISSN: 1477-9986
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 12
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    The economic effects of owner distance and local property management in US office markets (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: Using a large dataset of US offices we analyse the relationship between investors’ distance to their assets and the effective rent of these assets, and study the extent to which property managers can influence this relationship. We construct hedonic rent models to control for other known rent determinants. It turns out that proximity matters: holding everything else constant, investors located closely to their office buildings are able to extract significantly higher effective rents from these assets, especially if these buildings are of low quality. This effect is due to significant differences in occupancy levels. Interestingly, property managers can affect this relationship, mitigating the adverse effects of investor distance on effective office rents. Especially if the owner does not reside in the same state as the building, external property management is of importance, most prominently so for class-B office buildings.
    Keywords: G11 - Portfolio Choice ; Investment Decisions, R12 - Size and Spatial Distributions of Regional Economic Activity, R32 - Other Production and Pricing Analysis, R33 - Nonagricultural and Nonresidential Real Estate Markets
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    Topics: Geography , Economics
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  • 13
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    Exporting to Russia? Entry barriers for food suppliers in a territory in transition (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: This article draws on extensive fieldwork conducted in Central Asia to explore food exports to Russia. It takes its theoretical starting point in global value chain theory and pinpoints chain entry barriers relating to financing, transportation and standards. The article also proposes rethinking the aspects of territoriality and institutional context, and suggests their integration into one concept, or rather a process of contextualizing territories. In doing so, the article argues for a methodology that not only examines current events, but also captures change as particularly important in what we term the territory in transition examined here.
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    Topics: Geography , Economics
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  • 14
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    Regions and material flows: investigating the regional branching and industry relatedness of port traffics in a global perspective (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: This article proposes a quantitative analysis of the interdependencies between port specialization and regional specialization across the world. A global database is elaborated, covering about 360 port regions in both developed and developing countries. One goal is to verify how interdependent port traffic and regional characteristics are, in a context of increasingly flexible commodity and value chains. Despite the aggregated dimension of available data and the heterogeneity of local situations, the main results confirm the affinity between the primary sector and raw materials traffic, and between the tertiary sector and general cargo traffic, whereas the industrial sector offers mixed evidence. This allows us to address fundamental questions raised by both economic geography and regional science about transport and local development. The global typology of port regions points to certain regularities in their spatial distribution, and the article discusses the policy implications of particular cases.
    Keywords: L90 - General, O18 - Regional, Urban, and Rural Analyses, R40 - General
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    Topics: Geography , Economics
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  • 15
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    Towards a typology of repositioning strategies of GVC/GPN suppliers: the case of functional upgrading and downgrading (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: This article examines various upgrading and downgrading repositioning firm strategies within global value chains (GVCs) or global production networks (GPNs). It builds upon recent evidence that the mode of governance could vary profoundly among firms engaged in the same GVC/GPN. Therefore, the relevance of particular types of upgrading that were originally derived from the ideal types of GVC/GPN governance will be reconsidered. It is argued that the existing dissonance in the literature over possibilities for functional upgrading can be attributed to the different modes of governance that can exist within a particular GVC/GPN and to the diverse nature of functional upgrading. Consequently, a typology of functional upgrading is outlined, and it is argued that these different types vary significantly according to their probability and potential risk-benefit ratios. The article also introduces passive, adaptive and strategic downgrading and outlines their potential negative and positive effects on firms.
    Keywords: F63 - Economic Development, L23 - Organization of Production, L60 - General
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    Topics: Geography , Economics
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  • 16
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    Infrastructure as a traded product: A relational approach to finance in practice (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: As nations continue to grapple with growing infrastructure demand, financial markets will play an increasingly prominent role in the landscape for urban infrastructure. Yet existing literature tends to depict the ‘financialization’ of urban infrastructure assets as a restless move towards market efficiency aided by the growing transparency of financial information. This article offers a different view, showing how the spatial richness of financial data for infrastructure has progressed towards what we term a more permanent state of ‘informational translucency’. We draw on 53 interviews with participants in the market for infrastructure investment to present this more complicated picture of infrastructure finance, thereby elaborating a more granular understanding of how information flows through and shapes financial market geography. From this we propose a relational model that contributes to theoretical understandings of how financial products are intermediated over time and space.
    Keywords: O18 - Regional, Urban, and Rural Analyses
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    Topics: Geography , Economics
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  • 17
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    Islamic charitable infrastructure and giving in East London: Everyday economic-development geographies in practice (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: This article extends research exploring progressive models of reproducing economic life by reporting on research into some of the infrastructure, practices and motivations for Islamic charitable giving in London. In so doing the article: (i) makes visible sets of values, practices and institutions usually hidden in an otherwise widely researched international financial centre; (ii) identifies multiple, hard-to-research civic actors who are mobilising diverse resources to address economic hardship and development needs; and (iii) considers how these charitable values, practices and agents contribute to contemporary thinking about progressive economic possibilities.
    Keywords: D14 - Personal Finance, O12 - Microeconomic Analyses of Economic Development
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    Topics: Geography , Economics
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  • 18
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    Estimating the impact of Mexican drug cartels and drug-related homicides on crime and perceptions of safety (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: We estimate the impact of drug cartels and drug-related homicides on crime and perceptions of security in Mexico. Since the location where drug cartels operate might be endogenous, we combine the difference-in-difference estimator with instrumental variables. Using surveys on crime victimization we find that people living in areas that experienced drug-related homicides are more likely to take extra security precautions. Yet, these areas are also more likely to experience certain crimes, particularly thefts and extortions. In contrast, these crimes and perceptions of insecurity do not change in areas where cartels operate without leading to drug-related homicides.
    Keywords: C26 - Instrumental Variables (IV) Estimation, K49 - Other, R59 - Other
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    Topics: Geography , Economics
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  • 19
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    The evolution of municipal structure (2016)
    Oxford University Press
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    Publication Date: 2016-07-13
    Description: In most US states, urban expansions are governed by ‘popular determination’, under which residents make annexation decisions through referendum. Yet little research analyzes how urban spatial structure evolves under this system. We develop a model to examine how urban residents’ collective decisions on annexations and property taxes and their interactions with agricultural landowners affect municipal structure under popular determination. We find that the evolution process of an urban area can be divided into four stages similar to human life stages (infancy, juvenile, adulthood and maturity), characterized by the pace of development. The key parameters that determine urban spatial structure include agricultural rents, construction costs, interest rate, and the rate and uncertainty of income growth. Cities tend to be more spread-out and consist of a large number of smaller municipalities, in regions with lower agricultural land rents, lower construction costs, and lower rate and uncertainty of income growth.
    Keywords: H73 - Interjurisdictional Differentials and Their Effects, Q24 - Land, R14 - Land Use Patterns
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    Topics: Geography , Economics
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  • 20
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    Microscopy in this issue (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Print ISSN: 0022-0744
    Electronic ISSN: 1477-9986
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Published by Oxford University Press on behalf of The Japanese Electron Microscopy Society.
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  • 21
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    Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: Polysaccharides account for more than 90% of the content of fungal cell walls, but the mechanism underlying the formation of the architecture of the cell walls, which consist of microfibrils embedded in an amorphous wall matrix, remains unknown. We used electron microscopy to investigate ten different fungal cell-wall polysaccharides to determine whether they could self-assemble into the fibrillar or amorphous component of fungal cell walls in a test tube without enzymes. The ultrastructures formed by precipitating β-1,3-glucan and β-1,6-glucan are different depending on the existence of branching in the molecule. Linear β-1,3-glucan and linear β-1,6-glucan precipitate into a fibrillar ultrastructure. Branched β-1,6-glucan, mannan and glycogen precipitates are amorphous. Branched β-1,3-glucan forms a fibrillar plus amorphous ultrastructure. Self-assembly among combinations of different linear and branched cell-wall polysaccharides results in an ultrastructure that resembles that of a yeast cell wall, which suggests that self-assembly of polysaccharides may participate in the development of the three-dimensional architecture of the yeast cell wall.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 22
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    Endocytic mechanism of transferrin-conjugated nanoparticles and the effects of their size and ligand number on the efficiency of drug delivery (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: When considering drug delivery, the amount of drug that can be carried at a particular time and how the drug is incorporated efficiently into cells are important parameters. Transferrin (Tf)-conjugated nanocarriers have been used for the targeted delivery of drugs to cancer cells due to the availability of receptor-mediated clathrin-dependent endocytosis. In general, however, endocytosis seems to differ according to the size and shape of carriers. Large substances are generally internalized into cells by phagocytosis. We studied the internalization mechanism of Tf-conjugated nanoparticles (diameter, 522 nm). Tf-conjugated polystyrene particles were incorporated into cells by receptor-mediated endocytosis with large clathrin-coated vesicles even though their diameter was 〉500 nm and despite that fact that clathrin-coated vesicles have a diameter of 100 nm. This finding suggests that signals for internalization generated by stimulated Tf receptors (TfRs) activate clathrin-mediated endocytosis preferentially. Whether these larger particles could deliver drugs more efficiently than smaller particles was then examined. The toxicity of larger Tf-conjugated biodegradable nanoparticles (poly(lactic-co-glycolic acid)) encapsulating doxorubicin (diameter, 216 ± 38 nm) was appreciably dependent on the number of Tf molecules conjugated on a particle and the number of TfRs expressed on the cell membrane. Larger Tf-conjugated particles delivered drugs to cancer cells expressing many TfRs more selectively than their smaller counterparts (diameter, 56 ± 9 nm) if they were decorated with an appropriate number of Tf molecules.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 23
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    Imaging and diffraction of protein crystallization using TEM (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: Structural biology relies on good-quality protein crystals in order for structure determination. Many factors affect the growth process of a protein crystal including the way it nucleates and the types of damage and contamination during its growth. Although the nucleation process and quality of a crystal is vital to structure determination, they are both under-studied areas of research. Our research begins to explore ways of measuring the quality of protein crystals, using TEM, thus overcoming the problems associated with viewing wet specimens in a vacuum. Our current understanding of nucleation is that it is a two-step mechanism involving the formation of nuclei from dense liquid clusters; however; it is still unclear whether nuclei first start as amorphous aggregates or as crystalline lattices. Potentially, electron diffraction may be capable of uncovering this process. Using TEM imaging and diffraction of lysozyme as a model protein crystal, we report the internal two-dimensional strain and the density of crystallites in a protein crystal, at a resolution never seen before. The TEM diffraction shows unique features of crystal mosaicity that can be directly correlated to TEM images.
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  • 24
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    Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination in Lotus miyakojimae seeds (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: The cotyledon of legume seeds is a storage organ that provides nutrients for seed germination and seedling growth. The spatial and temporal control of the degradation processes within cotyledons has not been elucidated. Calcium oxalate (CaOx) crystals, a common calcium deposit in plants, have often been reported to be present in legume seeds. In this study, micro-computed tomography (micro-CT) was employed at the SPring-8 facility to examine the three-dimensional distribution of crystals inside cotyledons during seed maturation and germination of Lotus miyakojimae (previously Lotus japonicus accession Miyakojima MG-20). Using this technique, we could detect the outline of the embryo, void spaces in seeds and the cotyledon venation pattern. We found several sites that strongly inhibited X-ray transmission within the cotyledons. Light and polarizing microscopy confirmed that these areas corresponded to CaOx crystals. Three-dimensional observations of dry seeds indicated that the CaOx crystals in the L. miyakojimae cotyledons were distributed along lateral veins; however, their distribution was limited to the abaxial side of the procambium. The CaOx crystals appeared at stage II (seed-filling stage) of seed development, and their number increased in dry seeds. The number of crystals in cotyledons was high during germination, suggesting that CaOx crystals are not degraded for their calcium supply. Evidence for the conservation of CaOx crystals in cotyledons during the L. miyakojimae germination process was also supported by the biochemical measurement of oxalic acid levels.
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  • 25
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    Optimal accelerating voltage for HRTEM imaging of zeolite (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: The electron irradiation damage of MFI-type zeolite was estimated under various accelerating voltages of 100, 200 and 300 kV from successively captured high-resolution transmission electron microscopy (HRTEM) images. To determine the optimal accelerating voltage for HRTEM imaging of electron-sensitive MFI zeolite, the critical dose was estimated from the disappearance of a specific fast Fourier transform spot calculated from experimental images. Based only on the electron dose, a higher voltage was more advantageous. However, taking into account the minimum dose for imaging with a CCD camera, the optimal accelerating voltage for imaging MFI zeolite was 200 kV. The minimum dose for image detection with a CCD camera was surmised from the output/input signal ratio dependence on the accelerating voltage and the contrast range in simulated HRTEM images.
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  • 26
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    Electron holography of magnetic field generated by a magnetic recording head (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: The magnetic field generated by a magnetic recording head is evaluated using electron holography. A magnetic recording head, which is connected to an electric current source, is set on the specimen holder of a transmission electron microscope. Reconstructed phase images of the region around the magnetic pole show the change in the magnetic field distribution corresponding to the electric current applied to the coil of the head. A simulation of the magnetic field, which is conducted using the finite element method, reveals good agreement with the experimental observations.
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  • 27
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    A new grating X-ray spectrometer for 2-4 keV enabling a separate observation of In-L{beta} and Sn-L{alpha} emissions of indium tin oxide (2013)
    Oxford University Press
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    Publication Date: 2013-06-12
    Description: A new multilayer-coated varied line-spaced grating, JS4000, was fabricated and tested for extending the upper limit of a grating X-ray spectrometer for electron microscopy. This grating was designed for 2–3.8 keV at a grazing incidence angle of 1.35°. It was revealed that this new multilayer structure enables us to take soft-X-ray emission spectra continuously from 1.5 to 4.3 keV at the same optical setting. The full-width at half maximum of Te-L α1,2 (3.8 keV) emission peak was 27 eV. This spectrometer was applied to indium tin oxide particles and clearly resolved Sn-L α (3444 eV) and In-L β1 (3487 eV) peaks, which could not be resolved by a widely used energy-dispersive X-ray spectrometer.
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    Microscopy in this issue (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
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    Microscopy Hacks: development of various techniques to assist quantitative nanoanalysis and advanced electron microscopy (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: For development of advanced materials, characterization using a scanning transmission electron microscope (STEM) including analysis via X-ray energy-dispersive spectrometry and electron energy-loss spectrometry is essential. Recent advances in aberration-corrected instruments have offered large-scale data acquisition at a high resolution for limited acquisition times both in imaging and in analysis. Further advanced procedures are required to analyze such large-scale datasets more efficiently including quantification. In addition, more simplified tuning procedures are crucial to the best possible resolution in the latest aberration-corrected instruments. In this review article, several approaches to perform advanced electron microscopy, which the author has been developing with his colleague, are described as ‘Microscopy Hacks’. These are (i) quantification and elemental/chemical-imaging procedures, (ii) advanced statistical approaches to handle large-scale datasets and (iii) instrument characterization and tuning procedures including the latest development of an ad hoc autotuning procedure for aberration-corrected STEM imaging.
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    A new type of molybdenum oxide crystal encapsulated inside a single-walled carbon nanotube (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: The crystal structure of a new type of molybdenum oxide crystal encapsulated in a single-walled carbon nanotube (CNT) was examined via diffraction and spectroscopic techniques using both X-rays and electron beams. This new type of molybdenum oxide crystal has a chemical bonding state of MoO 3 , as confirmed by X-ray absorption spectroscopy, and the MoO 3 units exhibit axial symmetry, as clarified by electron diffraction from bundled and individual CNTs encapsulating the crystal. To obtain three-dimensional information on the structure, a cross-sectional sample was prepared using a conventional dimple and ion-mill method. High-resolution transmission electron microscopy images exhibit ring-like shapes that originated from the arrangement of the MoO 3 units inside the CNTs, as observed along the tube axis. The units are spaced 0.36 nm from each other in a ring arrangement and the distance between each ring is 0.391 nm.
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    Transmission electron microtomography in soft materials (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: This review summarizes the recent advances in three-dimensional (3D) imaging techniques and their application to polymer nanostructures, for example, microphase-separated structures of block copolymers. We place particular emphasis on the method of transmission electron microtomography (electron tomography for short; hereafter abbreviated as ET). As a result of recent developments in ET, truly quantitative 3D images of polymer nanostructures can now be obtained with subnanometer resolution. The introduction of scanning optics in ET has made it possible to obtain large amounts of 3D data from micrometer-thick polymer specimens by using conventional electron microscopes at a relatively low accelerating voltage, 200 kV. Thus, ET covers structures over a wide range of thicknesses, from a few nanometers to several hundred nanometers, which corresponds to quite an important spatial range for hierarchical polymer nanostructures. ET provides clear 3D images and a wide range of new structural information that cannot be obtained using other methods. Information traditionally derived from conventional microscopy or scattering methods can be directly acquired from 3D volume data. ET is a versatile technique that is not restricted to only polymer applications; it can also be used as a powerful characterization tool in energy applications such as fuel cells.
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    Performances of an 80-200 kV microscope employing a cold-FEG and an aberration-corrected objective lens (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: The performances of a newly developed 80–200 kV cold field emission gun (CFEG) transmission electron microscope (TEM) integrating a spherical aberration corrector for a TEM image-forming lens have been evaluated. To begin, we show that the stability of both emission and probe currents makes use of this new CFEG much friendlier. The energy spread of electrons emitted from the CFEG has been measured as a function of emission current and shows a very last 0.26 eV energy resolution at 200 kV and even 0.23 eV at 80 kV. The combination of the CFEG and the CEOS™ aberration corrector, associated with enhanced mechanical and electrical stabilities of this new microscope, allows reaching an information transfer below 75 pm at 200 and 80 pm at 80 kV. This unseen resolution at 200 kV has allowed us to study the structure of CoPt nanoparticles by observing direct images of their atomic arrangement along the high indexes zone axis. We have evidenced the presence of defects in these nanostructures that are not parallel to the electron beam. The precise stoichiometry of two iron oxides, FeO and Fe 2 O 3 , has been determined from an analysis of iron valence state that was obtained from a direct analysis of EELS fine structures spectrum of the two oxides.
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    Multi-reference-based multiple alignment statistics enables accurate protein-particle pickup from noisy images (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: Data mining from noisy data/images is one of the most important themes in modern science and technology. Statistical image processing is a promising technique for analysing such data. Automation of particle pickup from noisy electron micrographs is essential, especially when improvement of the resolution of single particle analysis requires a huge number of particle images. For such a purpose, reference-based matching using primary three-dimensional (3D) model projections is mainly adopted. In the matching, however, the highest peaks of the correlation may not accurately indicate particles when the image is very noisy. In contrast, the density and the heights of the peaks should reflect the probability distribution of the particles. To statistically determine the particle positions from the peak distributions, we have developed a density-based peak search followed by a peak selection based on average peak height, using multi-reference alignment (MRA). Its extension, using multi-reference multiple alignment (MRMA), was found to enable particle pickup at higher accuracy even from extremely noisy images with a signal-to-noise ratio of 0.001. We refer to these new methods as stochastic pickup with MRA (MRA-StoPICK) or with MRMA (MRMA-StoPICK). MRMA-StoPICK has a higher pickup accuracy and furthermore, is almost independent of parameter settings. They were successfully applied to cryo-electron micrographs of Rice dwarf virus. Because current computational resources and parallel data processing environments allow somewhat CPU-intensive MRA-StoPICK and MRMA-StoPICK to be performed in a short period, these methods are expected to allow high-resolution analysis of the 3D structure of particles.
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    Immunohistochemistry and ultrastructural characteristics of nerve endings in the oral mucosa of rat (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: The sensory nerve endings of the rat tongue, cheek and palate were studied using immunohistochemical staining and transmission electron microscopy analysis. The specimens were fixed in modified Karnovsky solution and embedded in Spurr resin. Substance P, calcitonin gene-related peptide (CGRP)- and protein gene product 9.5 (PGP b9.5)-containing nerve fibers in the rat tongue, cheek and palate were examined by electronic microscopical analysis and immunohistochemical localization. These fibers run very close to the basal lamina of the epithelium and extend into the filliform and fungiform papillae. Numerous plexiform fibers immunoreactive for substance P, CGRP and PGP 9.5 were found in the connective tissue of mucosa. Electron microscopic observations showed clearly immunostained nerve fibers, which are located very close to the basal lamina of epithelial cells. Some electron-dense granules may be observed in the axoplasms of both substance P and CGRP immunoreactive fibers. Several lamellar corpuscles into the subepithelial connective tissue papillae, Merkel corpuscles and numerous thin unmyelinated and myelinated axons were observed. The terminal axons revealed numerous mitochondria, neurofilaments, microtubules and clear vesicles in the base of axoplasmic protrusions. The lamellar cells showed caveolae and interlamelar spaces filled by amorphous substance. Between the lamellar cells and axoplasmic membrane, and in the adjacent lamellae region, desmosome-type junctions were observed. The quantitative and morphometric analysis showed nerve endings with an average area of 4.83 ± 3.4 μm 2 and 19.4 internal mitochondria in this site and the organized corpuscles with an average area of 79.24 ± 27.24 μm 2 and 24.23 internal mitochondria in this place. All the structures observed are involved in the transmission of pain and mechanoreceptors stimulus of these oral mucosae.
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    A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form in Cryptococcus neoformans treated with the microtubule inhibitor Nocodazole or with the drug solvent dimethyl sulfoxide (DMSO) or grown in yeast extract peptone dextrose (YEPD) medium, but they are absent in cells treated with Latrunculin A. Perinuclear F-actin rings may function as ‘funicular cabin’ for the cell nucleus, and actin cables as intracellular ‘funicular’ suspending nucleus in the central position in the cell and moving nucleus along the polarity axis along actin cables.
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    Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.
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    Ultrathin specimen preparation by a low-energy Ar-ion milling method (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
    Description: The low-energy Ar-ion milling method was used to prepare ultrathin specimens for transmission electron microscope observation. The samples were thinned initially by a usual focused ion beam technique or typical Ar-ion milling with a high energy of 2–10 keV and were thinned additionally by an Ar-ion beam with an energy less than 1 keV, typically 500–900 eV. This low-energy ion beam was scanned over the specimen, and secondary electrons induced by the ion beam could be detected to form secondary electron images with a resolution of a few micrometre. Because a desired area can be selected and thinned by the low-energy ion beam, redeposition or cross contamination from irradiation of a metal grid that supports the sample can be prevented. It was shown that the low-energy Ar-ion beam thins a surface amorphous damage layer preferentially and effectively rather than a crystal specimen. Images from ultrathin specimens of two different materials revealed a detailed structure.
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    Subscription Page (2013)
    Oxford University Press
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    Publication Date: 2013-04-02
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    Cover Page (2013)
    Oxford University Press
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    Subscription Page (2015)
    Oxford University Press
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    Publication Date: 2015-04-03
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    Possibility of high-resolution ptychographic iterative imaging with low energy electrons: dynamical calculations (2015)
    Oxford University Press
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    Publication Date: 2015-04-03
    Description: Ptychographic iterative engine (PIE), a form of coherent diffractive imaging in which the phase of both low-angle and high-angle diffraction data is used to form an image without a lens, can in principle deliver wavelength-limited resolution. Working at low accelerating voltages (30 keV) reduces specimen knock-on damage, increases high-resolution contrast and allows a scanning electron microscope to be used as a transmission electron microscopy. However, electrons are very strongly scattered at low energy. To that end, the effect of dynamical scattering on PIE at low accelerating voltages is calculated to illustrate the possibility of generating high-resolution images with low energy electrons.
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    Railroads, factor channelling and increasing returns: Cleveland and the emergence of the American manufacturing belt (2015)
    Oxford University Press
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    Publication Date: 2015-04-14
    Description: American Manufacturing Belt (AMB) emergence has been used by NEG theorists as a prime example of how increasing returns foster industrial concentration. Other studies suggest the AMB was in place before increasing returns became established. This study examines this previously unrecognized contradiction. An analysis of Cleveland, one of the fastest growing of AMB cities, is undertaken using new data sources. This finds the railroad sector crucial in generating direct employment and stimulating related industrial investment through forward and backward linkages. The former are associated with ‘factor channeling’—planned strategies to direct raw material flows to the city. NEG theorists’ under-emphasis on raw material provision and their use of the iceberg model to avoid analysis of the railroad sector is therefore found to be erroneous. The increasing returns hypothesis is evaluated using new data for several industrial sectors and rejected as a valid explanation for early manufacturing growth in Cleveland.
    Keywords: N61 - U.S. ; Canada: Pre-1913, N71 - U.S. ; Canada: Pre-1913, R15 - Econometric and Input-Output Models ; Other Models
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    Topics: Geography , Economics
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    Global pipelines for innovation: insights from the case of Norway (2015)
    Oxford University Press
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    Publication Date: 2015-04-14
    Description: Using data on 418 Norwegian firms, the results confirm the hypotheses that innovative/radically innovative firms tend to be more involved in international personal and formal networks than non-innovative/incrementally innovative ones. While regional and national networks are much more widespread than international ones, they are not significantly positively associated with innovation. International personal networks and international links with suppliers and customers and with universities and research institutions, as well as global buzz with strangers, are positively related to innovation. This suggests that innovation management and policy, in particular in countries with a limited national innovation base, could benefit from facilitating certain international networks.
    Keywords: O31 - Innovation and Invention: Processes and Incentives, R00 - General
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    Topics: Geography , Economics
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    Culture, spatial diffusion of ideas and their long-lasting imprints--evidence from Froebel's kindergarten movement (2015)
    Oxford University Press
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    Publication Date: 2015-04-14
    Description: We document the spatial diffusion of Friedrich Froebel’s radical invention of kindergartens in 19th-century Germany. The first kindergarten was founded at Froebel’s birthplace. Early spatial diffusion can be explained by cultural proximity, measured by historical dialect similarity, to Froebel’s birthplace. This result is robust to the inclusion of higher order polynomials in geographic distance and similarity measures with respect to industry, geography or religion. Our findings suggest that a common cultural basis facilitates the spill-over of ideas. We further show that the contemporaneous spatial pattern of child care coverage is still correlated with cultural similarity to Froebel’s place of birth.
    Keywords: J13 - Fertility ; Family Planning ; Child Care ; Children ; Youth, N33 - Europe: Pre-1913, Z13 - Economic Sociology ; Economic Anthropology
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    Local warming and violent conflict in North and South Sudan (2015)
    Oxford University Press
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    Publication Date: 2015-04-14
    Description: Our article contributes to the emerging micro-level strand of the literature on the link between local variations in weather shocks and conflicts by focusing on a pixel-level analysis for North and South Sudan between 1997 and 2009. Temperature anomalies are found to strongly affect the risk of conflict, whereas the risk is expected to magnify in a range of 24–31% in the future under a median scenario. Our analysis also sheds light on the competition over natural resources, in particular water, as the main driver of such relationship in a region where pastoralism constitutes the dominant livelihood.
    Keywords: D74 - Conflict ; Conflict Resolution ; Alliances, O13 - Agriculture ; Natural Resources ; Energy ; Environment ; Other Primary Products, Q54 - Climate ; Natural Disasters ; Global Warming, R11 - Regional Economic Activity: Growth, Development, and Changes
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    Application of multispectral imaging detects areas with neuronal myelin loss, without tissue labelling (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: The application of multispectral imaging to discriminate myelinated and demyelinated areas of neural tissue is herein presented. The method is applied through a custom-made, multispectral imaging monochromator, coupled to a commercially available microscope. In the present work, a series of spinal cord sections were analysed derived from mice with experimental autoimmune encephalomyelitis (EAE), an experimental model widely used to study multiple sclerosis (MS). The multispectral microscope allows imaging of local areas with loss of myelin without the need of tissue labelling. Imaging with the aforementioned method and system is compared in a parallel way with conventional methods (wide-field and confocal fluorescence microscopies). The diagnostic sensitivity of our method is 90.4% relative to the ‘gold standard’ method of immunofluorescence microscopy. The presented method offers a new platform for the possible future development of an in vivo , real-time, non-invasive, rapid imaging diagnostic tool of spinal cord myelin loss-derived pathologies.
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    Recent advancement in the challenges to connectomics (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: Advancement of microscopic technologies established significant progress in our understanding of the brain. In the recent effort to elucidate the complete wiring map of the brain circuitry termed ‘connectome’, the different modalities of imaging technology, including those of light and electron microscopy, have started providing essential contribution in multiple organisms. The contribution would be impossible without the recent innovation in both acquisition and analyses of the big connectomic data. The current data demonstrated complicated networks with unidirectional and reciprocal connections of the cerebral circuits at the macroscopic and light microscopic (‘mesoscopic’) levels, and the unimaginable complexity of synaptic connections between axons and dendrites at the electron microscopic (‘microscopic’) level. At the same time, the data highlighted the necessity to make substantial advancement in methodology of the connectomic studies, including efficient handling and automated analyses of the acquired dataset. Further understanding about structural and functional connectome seems to be facilitated by combinations of the different imaging modalities. Such multidisciplinary approaches will give us the clues to address whether the complete connectome can elucidate fundamental mechanisms processing the basic and higher functions of human brains.
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    Quantitative determination of occupation sites of trace Co substituted for multiple Fe sites in M-type hexagonal ferrite using statistical beam-rocking TEM-EDXS analysis (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: The occupation sites and the occupancies of trace dopants in La/Co co-doped Sr-M-type ferrite, SrFe 12 O 19 , were quantitatively and precisely determined by beam-rocking energy-dispersive X-ray spectroscopy (EDXS) on the basis of electron-channeling effects. Because the Co atoms, in particular, should be partially substituted for the five crystallographically inequivalent sites, which could be key parameters in improving the magneto-crystalline anisotropy, it is difficult yet intriguing to discover their occupation sites and occupancies without using the methods of large-scale facilities, such as neutron diffraction and synchrotron radiation. In the present study, we tackled this problem by applying an extended statistical atom location by channeling enhanced microanalysis method, using conventional transmission electron microscopy, EDXS and dynamical electron elastic/inelastic scattering theories. The results show that the key occupation sites of Co were the 2a, 4f 1 and 12k sites. The quantified occupancies of Co were consistent with those of the previous study, which involved a combination of neutron diffraction and extended X-ray absorption fine structure analysis, as well as energetics considerations based on by first-principles calculations.
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    Effects of isoconcentration surface threshold values on the characteristics of needle-shaped precipitates in atom probe tomography data from an aged Al-Mg-Si alloy (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: Needle-shaped precipitates in an aged Al–0.62Mg–0.93Si (mass%) alloy were identified using a compositional threshold method, an isoconcentration surface, in atom probe tomography (APT). The influence of thresholds on the morphological and compositional characteristics of the precipitates was investigated. Utilizing optimum parameters for the concentration space, a reliable number density of the precipitates is obtained without dependence on the elemental concentration threshold in comparison with evaluation by transmission electron microscopy (TEM). It is suggested that careful selection of the concentration space in APT can lead to a reasonable average Mg/Si ratio for the precipitates. It was found that the maximum length and maximum diameter of the precipitates are affected by the elemental concentration threshold. Adjustment of the concentration threshold gives better agreement with the precipitate dimensions measured by TEM.
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    Two-dimensional crystal structure of aquaporin-4 bound to the inhibitor acetazolamide (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: Acetazolamide (AZA) reduces the water permeability of aquaporin-4, the predominant water channel in the brain. We determined the structure of aquaporin-4 in the presence of AZA using electron crystallography. Most of the features of the 5-Å density map were consistent with those of the previously determined atomic model. The map showed a protruding density from near the extracellular pore entrance, which most likely represents the bound AZA. Molecular docking simulations supported the location of the protrusion as the likely AZA-binding site. These findings suggest that AZA reduces water conduction by obstructing the pathway at the extracellular entrance without inducing a large conformational change in the protein.
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    In situ heating SEM observation of the bainitic transformation process in Cu-17Al-11Mn (at.%) alloys (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: To understand the bainitic transformation behavior in Cu–17Al–11Mn (at.%) alloys, dynamic in situ observation during heating was carried out in a scanning electron microscope (SEM). In this study, after optimizing the sample preparation method and observation conditions, we successfully observed the transformation process with sufficient resolution and contrast. From the observation results, bainite is first formed preferentially at the grain boundaries of the parent phase. Bainite is also formed inside the grains to relax the elastic strain generated by the initial bainite. Regarding the growth mode, in the early stage of the transformation, bainite grows along the longitudinal direction, and in the late stage, it grows along the lateral direction. The growth rate of the bainite was also evaluated by continuous observation of the same plate. Dynamic in situ observation of a martensitic transformation in the same alloy was also performed to compare the growth mode with that of bainite, and it was found that the behavior is considerably different between bainitic and the martensitic transformations.
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    Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: Although many studies of the Golgi apparatus structure have been performed by light and electron microscopy, the full shape of the Golgi apparatus remained unclear due to the technical limitations of the previously applied microscopy techniques. In this study, we used serial section scanning electron microscopy (SEM) for the morphological study of the Golgi apparatus. This method is useful for three-dimensional (3D) reconstruction of cellular structures without requiring specialized instruments, unlike focused ion beam SEM (FIB-SEM) and serial block face SEM (SBF-SEM). Using the serial section SEM method developed by our laboratory, we investigate the 3D shape of the osmium-impregnated Golgi apparatus in rat epididymal cells, pancreatic acinar cells and gonadotropes. The combination of serial section SEM and a 3D reconstruction technique enabled us to elucidate the entire shape of the Golgi apparatus in these cells. The full shape of the Golgi apparatus in epididymal cells formed a basket-like structure with oval-shaped cisterns, while the Golgi apparatus in an acinar cell from the pancreas was composed of elongated ribbon-like structures that were connected to each other, making a coarse network. The overall image of the Golgi apparatus cisterns from a gonadotrope looked like a spherical cage. This study has clearly shown that entire 3D shape of the Golgi apparatus varies depending on the cell type and that the Golgi cisterns network appears as a single mass located in the large region of the cytoplasm.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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    9 + 0 and 9 + 2 cilia are randomly dispersed in the mouse node (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: The initial determination of left–right asymmetry is an essential process in embryonic development. In mouse embryo, cilia in the node play an important role generating the nodal flow that subsequently triggers left–right determination in the embryo. Although nodal cilia have historically been thought to have a 9 + 0 axonemal configuration, the existence of 9 + 2 cilia has been reported so far. Because the distribution of those two types of cilia within the node has not yet been reported, we assessed the arrangement of 9 + 0 and 9 + 2 cilia in the node. In this study, we concluded that most of the nodal cilia were 9 + 0 in structure and there were much fewer 9 + 2 cilia than 9 + 0 cilia. Furthermore, the two types of cilia were randomly distributed in the node with no regularity. In addition, we studied the embryonic origin of the crown cells surrounding the node to better understand their identity.
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    Sub-nm 3D observation of human hair melanin by high-voltage STEM (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: The ultrastructure of melanin granules in human hair was studied using 1,000 kV high-voltage scanning transmission electron microscopy to successfully reconstruct three-dimensional images of the whole melanin granule. It was revealed that the melanin granule was composed of a membrane-like outer structure that included many spherical vesicles, and an inner matrix containing a sheet-like structure in the elongated direction of the melanin granule and a sheet-like arrays structure in the cross direction. The outer structure of the melanin granule was maintained even after exposure to hair-bleaching agents to decompose the melanin granule, suggesting that the outer structure was a highly robust structure and composition compared with the inner matrix .
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    Effect of Ti diffusion on the microstructure of Ge2Sb2Te5 in phase-change memory cell (2015)
    Oxford University Press
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    Publication Date: 2015-12-13
    Description: The dependence of the microstructure of Ge 2 Sb 2 Te 5 (GST) on Ti diffusion into GST by annealing in GST/Ti/TiN phase-change random access memory stack was studied by various transmission electron microscopy (TEM) techniques. The microstructure and crystal structure of GST were identified with high-resolution TEM (HRTEM) and image simulation technique, and the Ti diffusion into GST was revealed by scanning transmission electron microscope-energy-dispersive X-ray spectroscopy analysis. It was observed that Ti atoms of Ti/TiN thin layers were incorporated into GST cell through several thermal annealing steps and they could retard the phase transition from face-centered cubic (FCC) phase into hexagonal close-packed (HCP) phase partially and restrain the increase in grain size. Thus, it is concluded that Ti diffusion can affect the microstructure of GST including the type of the crystal phase and grain size of GST. It was shown that the insertion of diffusion barrier between TiN and GST could block Ti diffusion into GST and make it possible for FCC phase to completely transform into HCP phase.
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    High-resolution imaging by scanning electron microscopy of semithin sections in correlation with light microscopy (2015)
    Oxford University Press
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    Publication Date: 2015-12-13
    Description: In this study, we introduce scanning electron microscopy (SEM) of semithin resin sections. In this technique, semithin sections were adhered on glass slides, stained with both uranyl acetate and lead citrate, and observed with a backscattered electron detector at a low accelerating voltage. As the specimens are stained in the same manner as conventional transmission electron microscopy (TEM), the contrast of SEM images of semithin sections was similar to TEM images of ultrathin sections. Using this technique, wide areas of semithin sections were also observed by SEM, without the obstruction of grids, which was inevitable for traditional TEM. This study also applied semithin section SEM to correlative light and electron microscopy. Correlative immunofluorescence microscopy and immune-SEM were performed in semithin sections of LR white resin-embedded specimens using a FluoroNanogold-labeled secondary antibody. Because LR white resin is hydrophilic and electron stable, this resin is suitable for immunostaining and SEM observation. Using correlative microscopy, the precise localization of the primary antibody was demonstrated by fluorescence microscopy and SEM. This method has great potential for studies examining the precise localization of molecules, including Golgi- and ER-associated proteins, in correlation with LM and SEM.
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    Dynamical observation of lithium insertion/extraction reaction during charge-discharge processes in Li-ion batteries by in situ spatially resolved electron energy-loss spectroscopy (2015)
    Oxford University Press
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    Publication Date: 2015-12-13
    Description: All-solid-state Li-ion batteries (LIBs) with solid electrolytes are expected to be the next generation devices to overcome serious issues facing conventional LIBs with liquid electrolytes. However, the large Li-ion transfer resistance at the electrode/solid-electrolyte interfaces causes low power density and prevents practical use. In-situ -formed negative electrodes prepared by decomposing the solid electrolyte Li 1+ x +3 z Al x (Ti,Ge) 2– x Si 3 z P 3– z O 12 (LASGTP) with an excess Li-ion insertion reaction are effective electrodes providing low Li-ion transfer resistance at the interfaces. Prior to our work, however, it had still been unclear how the negative electrodes were formed in the parent solid electrolytes. Here, we succeeded in dynamically visualizing the formation by in situ spatially resolved electron energy-loss spectroscopy in a transmission electron microscope mode (SR-TEM-EELS). The Li-ions were gradually inserted into the solid electrolyte region around 400 nm from the negative current-collector/solid-electrolyte interface in the charge process. Some of the ions were then extracted in the discharge process, and the rest were diffused such that the distribution was almost flat, resulting in the negative electrodes. The redox reaction of Ti 4+ /Ti 3+ in the solid electrolyte was also observed in situ during the Li insertion/extraction processes. The in situ SR-TEM-EELS revealed the mechanism of the electrochemical reaction in solid-state batteries.
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    Quantitative annular dark-field imaging of single-layer graphene--II: atomic-resolution image contrast (2015)
    Oxford University Press
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    Publication Date: 2015-12-13
    Description: We have investigated how accurately atomic-resolution annular dark-field (ADF) images match between experiments and simulations to conduct more reliable crystal structure analyses. Quantitative ADF imaging, in which the ADF intensity at each pixel represents the fraction of the incident probe current, allows us to perform direct comparisons with simulations without the use of fitting parameters. Although the conventional comparison suffers from experimental uncertainties such as an amorphous surface layer and specimen thickness, in this study we eliminated such uncertainties by using a single-layer graphene as a specimen. Furthermore, to reduce image distortion and shot noises in experimental images, multiple acquisitions with drift correction were performed, and the atomic ADF contrast was quantitatively acquired. To reproduce the experimental ADF contrast, we used three distribution functions as the effective source distribution in simulations. The optimum distribution function and its full-width at half-maximum were evaluated by measuring the residuals between the experimental and simulated images. It was found that the experimental images could be explained well by a linear combination of a Gaussian function and a Lorentzian function with a longer tail than the Gaussian function.
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    Dual-phase steel structure visualized by extremely slow electrons (2015)
    Oxford University Press
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    Publication Date: 2015-12-13
    Description: Mechanical properties of complex steels are affected by their multi-phase structure. Scanning electron microscopy (SEM) is routinely used for characterizing dual-phase (DP) steels, although the identification of steel constituents is not straightforward. In fact, there are several ways of enabling the ferrite–martensite segmentation by SEM, and a wide range of electron energies can be utilized. This study demonstrates the phase identification of DP steels at high, low and extremely low landing energies of the primary electrons from tens of keV to tens of eV. Visualization of the specimen surface at very low landing energies has been achieved by inserting an earthed detector between the pole piece and the negatively biased specimen. This ‘cathode lens mode’ enables the use of the full energy range up to the primary electron energies. It has been found that extremely slow electrons (〈100 eV) are exceptionally suitable for separation of the martensite from the ferrite matrix due to high surface sensitivity, enabling visualization of very fine features. Moreover, the channelling contrast is significantly suppressed at the landing energy of tens of eV of the primary electrons, which enables separation of the phases clearly even in the images acquired at low magnification. The contrast between the phases at tens of eV can be explained by the different thickness of native oxide covering the martensite and the ferrite phase.
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    Electron microscope detection of tubular aggregates in the mosquito cell line C6/36 treated with Culex australicus (mosquito) homogenate (2015)
    Oxford University Press
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    Publication Date: 2015-12-13
    Description: ‘Tubular aggregates’ are morphologically distinct cytoplasmic structures that have been linked to a variety of pathological conditions. This report documents the presence of tubular aggregates in an insect cell line (C6/36 cells derived from Aedes albopictus ) following inoculation of the cells with material derived from cell culture passaged homogenized Culex australicus mosquitoes. The tubular aggregates were detected in ~2% of treated cells and had three morphological forms that were termed primary, secondary and tertiary, with progressively greater levels of structural complexity. The findings indicate that tubular aggregates can be induced in an insect cell culture system by an unidentified agent present in some mosquitoes.
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    Union operation image processing of data cubes separately processed by different objective filters and its application to void analysis in an all-solid-state lithium-ion battery (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: In this article, we propose a smart image-analysis method suitable for extracting target features with hierarchical dimension from original data. The method was applied to three-dimensional volume data of an all-solid lithium-ion battery obtained by the automated sequential sample milling and imaging process using a focused ion beam/scanning electron microscope to investigate the spatial configuration of voids inside the battery. To automatically fully extract the shape and location of the voids, three types of filters were consecutively applied: a median blur filter to extract relatively larger voids, a morphological opening operation filter for small dot-shaped voids and a morphological closing operation filter for small voids with concave contrasts. Three data cubes separately processed by the above-mentioned filters were integrated by a union operation to the final unified volume data, which confirmed the correct extraction of the voids over the entire dimension contained in the original data.
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    Direct observation of polyelectrolyte brushes under wet and dry conditions by atmospheric scanning electron microscopy (2016)
    Oxford University Press
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    Publication Date: 2016-03-31
    Description: Polyelectrolyte brushes are polyelectrolyte polymers with one end fixed to a substrate. In this study, direct nano-scale visualization of polyelectrolyte brushes was carried out under ‘aqueous conditions’ by atmospheric scanning electron microscopy. The thickness of the polyelectrolyte brush layer was measured under both dry and aqueous conditions, experimentally confirming the swollen state of the brushes. These experimental findings qualitatively agreed with the results from previous neutron reflectivity experiments using similar polyelectrolyte brushes. Such direct visualization of polymer brushes in real space opens up a new route for better understanding their surface properties, such as friction, adhesion and wettability.
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    Accuracy and efficiency in simulating equilibrium land-use patterns for self-organizing cities (2015)
    Oxford University Press
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    Publication Date: 2015-06-20
    Description: Lucas and Rossi-Hansberg (L&RH) (2002, Econometrica , 70: 1445–1476) and Fujita and Ogawa (F&O) (1982, Regional Science and Urban Economics , 12: 161–196, 1989, Environment and Planning A , 21: 363–374) develop urban models in which economic activity self-organizes due to spillovers in production. However, F&O (1982, Regional Science and Urban Economics , 12: 161–196, 1989, Environment and Planning A , 21: 363–374) show that rents and employment density are flat or falling as the city center is approached, while in the simulations of L&RH (2002, Econometrica , 70: 1445–1476), rents rise at an increasing rate toward the center suggesting a concentration of employment near the center. For the Lucas and Rossi-Hansberg model, we prove that land rents and density must be flat or falling near the center. We explain how using a polar coordinate system when approximating a two-dimensional integral can create systematic imprecision in their simulations, and then present revised simulations. The proofs and simulations suggest that in urban models where economic activity self-organizes firms do not unduly cluster at the center of a central business district even in monocentric equilibria.
    Keywords: R13 - General Equilibrium and Welfare Economic Analysis of Regional Economies, R14 - Land Use Patterns, R30 - General
    Print ISSN: 1468-2702
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    Topics: Geography , Economics
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    Scanning electron microscopy of scale microstructures in Channa barca, a poorly known snake head fish in regard to taxonomy (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: Scanning electron microscopy has been identified as an important approach in studying scale microstructures in fish with reference to taxonomy. In this article, a detailed microstructural analysis of the scales of Channa barca , a poorly known snakehead fish was carried out the location of focus, inter-radial distance, average width and inter-circular space of anterior circulii; inter-circular distance and dentition in lateral circulii; the shape, spacing, length of lepidonts in anterior circulii and the number and width of radii were compared with those of a related species, Channa aurantimaculata . The location of the focus was found to be similar to those of the gachua group of the genus Channa but was different from those of the marulius group. There were major similarities—though with a few notable differences—in scale microstructures between C. barca and the aforementioned closely related species C. aurantimaculata , indicating that scale microstructure analysis has the potential to distinguish even closely related fish species . While several of the microstructural features of the scale were found to be similar to those of the gachua group, others were closer in nature to those of the marulius group. Some microstructural characteristics, however, were found to be totally different from those of both gachua and marulius groups. All of these characteristic features of scale microstructure in C. barca are discussed with reference to taxonomic significance.
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    Subscription Page (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
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    Comparison of optical data from flow cytometry and microscopy of leukocytes after exposure to specific reagents (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: Flow cytometry and microscopy are equally important in cell analysis. However, few reports have compared the optical data (cell size, internal complexity and fluorescent signal) from flow cytometry and microscopy. In this study, we compared the scattergram from XN-series, a flow cytometry based hematology analyzer with microscopic images of similarly treated leukocytes, and investigated the correlation between the appearance in the scattergram and cell size, internal complexity and fluorescence intensity. Healthy human peripheral blood was analyzed using the XN analyzer. For microscopic comparison, five types of leukocytes (monocytes, lymphocytes, basophils, neutrophils and eosinophils) were isolated from the peripheral blood by centrifugation and magnetic cell sorting, treated with a specific reagent and analyzed using electron microscopy, laser microscopy and confocal laser microscopy. Cell size, residual internal structures and fluorescence intensity correlated with intensity of forward-scattering, side scattering and fluorescent light. In this study, optical data from a clinically used hematology analyzer was clarified using microscopic images.
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    Characterization of complex phase steel using backscattered electron images with controlled collection angles (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: For optimizing the microstructure of complex phase (CP) steels, characterization using scanning electron microscopy (SEM) is powerful because it allows observations from very low to high magnification. SEM specimens of steels are often etched in order to distinguish between the different phases by producing topographic information. This is however an ‘indirect’ method of characterization, which does not give precise structural information. We have developed a new technique for the selective imaging of the martensite (M) phase in a ferritic (F)–M complex phase steel. Backscattered electron (BSE) images at 15–20 kV were recorded by systematically changing the collection angle , where is measured from the specimen surface. When was 30–45°, strong channeling contrast was observed. For lower values of , it is the low energy loss electrons that mainly contribute to the contrast. As increases, the M phase exhibits brighter contrast. When exceeds 60°, a selective imaging of the M phase is achieved. This is not because martensite has a larger mean atomic number than ferrite, but is due to the fact that martensite has a high crystallographic defect density. Anomalously bright M contrast is due to multiple scattering of BSE due to the high density of planar defects and dislocations. Low angle BSE allows high resolution characterization of complex microstructures, while high angle BSE gives quantitative assessment of the distribution and the volume fraction of the martensite phase.
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    Quantitative analysis of angle-selective backscattering electron image of iron oxide and steel (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: The contrasts in backscattered electron (BSE) images, such as topographic, channeling and mean atomic number (Z) contrasts, were investigated quantitatively from the cross section of a heat-treated steel sheet using a scanning electron microscope (SEM). High primary electron energy ( E P ) enhances Z contrast, whereas low E P improves channeling contrast. A high take-off angle ( ; measured from the specimen surface) also enhances Z contrast, whereas low improves channeling contrast. When becomes very low, topographic information is enhanced and superimposed on channeling contrast due to the tilt effect of BSE. The relationship of the behaviors of the Z contrast and the channeling contrast can be understood by the detection ratio of low-loss electrons (LLEs) to the inelastic BSE components emitted from the sample surface; LLEs contribute to channeling contrast, and their ratio increases with decreasing E P and . The systematic results obtained in this study are useful for controlling SEM conditions in order to enhance the target information in BSE images for practical materials of interest.
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    Effects of non-rotationally symmetric aberrations on the quantitative measurement of lattice positions in a graphene monolayer using high-resolution transmission electron microscopy (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: It is crucial to determine the position of lattice atoms in a monolayer specimen with high precision using high-resolution transmission electron microscopy (HRTEM). Image simulations indicate that the intensity centers of periodic lattice atoms in graphene may deviate from their intrinsic positions if non-rotationally symmetric aberrations (except for three-fold and six-fold aberrations) exist in the HRTEM imaging system. In this letter, we quantitatively compared the deviations caused by non-rotationally symmetric aberrations, which are equivalent to individually produce a /4 phase shift in the wave aberration function at a given frequency of 7.2 nm –1 . A two-fold aberration caused a maximum shift of 0.3 Å, and in the images affected by the axial coma, graphene still maintained its hexagonal structure while all of the measured atomic positions deviated. Furthermore, we discovered that atoms on each sublattice tended to shift by similar distances in the image. Based on this rule, we retrieved the intrinsic bond length between neighboring carbon atoms by ‘shifting’ the measured atom positions in an experimental HRTEM image affected by residual aberrations.
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    Crack tip shielding observed with high-resolution transmission electron microscopy (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: The dislocation shielding field at a crack tip was experimentally proven at the atomic scale by measuring the local strain in front of the crack tip using high-resolution transmission electron microscopy (HRTEM) and geometric phase analysis (GPA). Single crystalline (110) silicon wafers were employed. Cracks were introduced using a Vickers indenter at room temperature. The crack tip region was observed using HRTEM followed by strain measurements using GPA. The measured strain field at the crack tip was compressive owing to dislocation shielding, which is in good agreement with the strain field calculated from elastic theory.
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    Local cluster symmetry of a highly ordered quasicrystalline Al58Cu26Ir16 extracted through multivariate analysis of STEM images (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: The structure of a highly ordered Al 58 Cu 26 Ir 16 decagonal quasicrystal (d-QC), which is constructed by a periodic stack of quasiperiodic atomic planes, was investigated using aberration-corrected scanning transmission electron microscopy. The entire quasiperiodic structure can be well described based on the pentagonal Penrose lattice decorated with a decagonal columnar cluster 2 nm across, and the individual clusters apparently showed up with localized atomic disorder to various degree that blurs the underlying cluster symmetry. Multivariate analysis of the cluster images with principal component analysis has successfully extracted two fundamental clusters, which are with definite mirror and 10-fold-rotation symmetries; this is the first solid example of the d-QC composed of multiple fundamental clusters with different symmetry. Consequently, it is found that all the observed variations can be reasonably interpreted by a linear combination between these two fundamental clusters of mirror and 10-fold symmetries, indicating that the possible atomic disorder is strongly restricted by these particular symmetries. Characteristic distributions of the mirror/10-fold clusters on the pentagonal Penrose lattice are also described.
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    Electron probe X-ray microanalysis studies on the distribution change of intra- and extracellular calcium in the elongation zone of horizontally reoriented soybean roots (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: To clarify the contribution of Ca to the gravitropic response, quantitative X-ray microanalyses were performed on cryosections of roots of soybean seedlings reoriented horizontally from their original vertical orientation. After reorientation, the roots bent gradually toward the ground at the elongation zone. The concentrations of Ca in the cell walls, cytoplasmic matrices and central vacuoles of cortical cells were measured in the upper and lower halves of the elongation zone at 0, 30, 60 and 120 min after reorientation. The Ca concentration did not significantly change in the cytoplasmic matrices or vacuoles. Additionally, the Ca concentration did not change significantly in cell walls at 30 min after reorientation; however, beyond 30 min, this concentration significantly increased gradually in the lower half of the elongation zone and decreased in the upper half of the elongation zone, indicating a typical asymmetrical distribution of Ca. These results suggest that Ca moves apoplastically in soybean roots to produce an asymmetrical Ca distribution in the elongation zone, which contributes to root curvature. The possible role of Ca in accelerating or repressing the effect of auxin is also discussed in this study.
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    Rapid contrast evaluation method based on affinity beads and backscattered electron imaging for the screening of electron stains (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: Uranyl salts are toxic and radioactive; therefore, several studies have been conducted to screen for substitutes of electron stains. In this regard, the contrast evaluation process is time consuming and the results obtained are inconsistent. In this study, we developed a novel contrast evaluation method using affinity beads and a backscattered electron image (BSEI), obtained using scanning electron microscopy. The contrast ratios of BSEI in each electron stain treatment were correlated with those of transmission electron microscopic images. The affinity beads bound to cell components independently. Protein and DNA samples were enhanced by image contrast treated with electron stains; however, this was not observed for sugars. Protein-conjugated beads showed an additive effect of image contrast when double-stained with lead. However, additive effect of double staining was not observed in DNA-conjugated beads. The varying chemical properties of oligopeptides showed differences in image contrast when treated with each electron stain. This BSEI-based evaluation method not only enables screening for alternate electron stains, but also helps analyze the underlying mechanisms of electron staining of cellular structures.
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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    Development of a novel straining holder for transmission electron microscopy compatible with single tilt-axis electron tomography (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
    Description: We have developed a newly designed straining specimen holder for in situ transmission electron microscopy (TEM) compatible with high-angle single tilt-axis electron tomography. The holder can deform a TEM specimen under tensile stress with the strain rate between 1.5 x 10 –6 and 5.2 x 10 –3 s –1 . We have also confirmed that the maximum tilt angle of the specimen holder reaches ±60° with a rectangular shape aluminum specimen. The new specimen holder, termed as ‘straining and tomography holder’, will have wide range potential applications in materials science.
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    Cover Page (2015)
    Oxford University Press
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    Publication Date: 2015-10-10
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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    Chicken or egg? The PVAR econometrics of transportation (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
    Description: To analyse the mutually dependent relationship between local economic performance, demand for and supply of transport services, we employ the structural panel VAR method that is popular in the macroeconomic literature, but has not previously been applied to the modelling of the within-city dynamics of transportation. We focus on a within-city panel of Berlin, Germany during the heyday of the construction of its dense public transit network (1890–1914). Our results suggest that economic outcomes and a supply of transport infrastructure mutually determine each other. We find a short-run (long-run) elasticity of property prices with respect to transport supply of 2% (8.5%). Both transport demand and supply seem to be driven more by firms than by residents.
    Keywords: N73 - Europe: Pre-1913, N74 - Europe: 1913-, R12 - Size and Spatial Distributions of Regional Economic Activity, R14 - Land Use Patterns, R41 - Transportation: Demand, Supply, and Congestion ; Safety and Accidents ; Transportation Noise
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    Topics: Geography , Economics
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    Transnational corporations shaping institutional change: the case of English law firms in Germany (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
    Description: Questions remain about the factors that influence the ability of transnational corporations (TNCs) to shape processes of institutional change. In particular, questions about power relations need more attention. To address such questions, this article develops a neo-institutional theory-inspired analysis of the case of English law firms and their impacts on institutional change in Germany. The article shows that the shaping of the direction of institutional change by English legal TNCs was a product of conjunctural moments in which local institutional instability combined with the presence, resources and strategies of the TNCs to redirect the path of institutional evolution. This draws attention to the need to go beyond the TNC and its resources and to consider the way a diverse array of local actors and their generating of instability in existing institutional structures influence the ability of TNCs to become involved in processes of institutional change in particular, conjunctural moments in time.
    Keywords: General, L84 - Personal, Professional, and Business Services, M16 - International Business Administration
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    Topics: Geography , Economics
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    Airport cities and metropolitan labor markets: an extension and response to Cidell (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
    Description: Using Census 2000 CTPP tract-level data for the 51 largest U.S. metropolitan areas, airport cities—agglomerations of employment anchored by airports—are placed in the context of metropolitan spatial form in order to understand their emergence and function. Major airports anchor significant concentrations of employment which average one-third to one-half the size of the respective CBDs, depending upon the operationalization, while 80% of the airports anchor employment agglomerations. Airport cities are anchored by airports but not driven by aviation. The relationship between spatial form and economic function suggests that need for airport access determines the location of transportation-providing employment while spatial employment filtering, based on urban land costs and agglomeration benefits, are responsible for the presence of transportation-supporting and transportation-using employment, such as producer services.
    Keywords: L93 - Air Transportation, O18 - Regional, Urban, and Rural Analyses, R12 - Size and Spatial Distributions of Regional Economic Activity
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    Topics: Geography , Economics
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    The role of major infrastructure in subregional economic development: an empirical study of airports and cities (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
    Description: The link between airports, air service and regional economic development has been well-established and used to justify airport expansion at the expense of local communities because of subsequent region-wide benefits. However, local-level spatial analyses based on US Economic Census data indicate that economic benefits in terms of professional and administrative employment do not necessarily offset local economic and quality of life costs. Furthermore, arguments for an airport city or aerotropolis phenomenon in the US context ignore the individual histories and morphologies of metropolitan areas and overstate the influence an airport has on the economic development of its region.
    Keywords: L93 - Air Transportation, O18 - Regional, Urban, and Rural Analyses, R41 - Transportation: Demand, Supply, and Congestion ; Safety and Accidents ; Transportation Noise, R53 - Public Facility Location Analysis ; Public Investment and Capital Stock
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    Topics: Geography , Economics
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    Cities and product variety: evidence from restaurants (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
    Description: This article measures restaurant variety in US cities and argues that city structure directly increases product variety by spatially aggregating demand. I discuss a model of entry thresholds in which market size is a function of both population and geographic space and evaluate implications of this model with a new data set of 127,000 restaurants across 726 cities. I find that geographic concentration of a population leads to a greater number of cuisines and the likelihood of having a specific cuisine is increasing in population and population density, with the rarest cuisines found only in the biggest, densest cities. Further, there is a strong hierarchical pattern to the distribution of variety across cities in which the specific cuisines available can be predicted by the total count. These findings parallel empirical work on Central Place Theory and provide evidence that demand aggregation has a significant impact on consumer product variety.
    Keywords: L10 - General, R12 - Size and Spatial Distributions of Regional Economic Activity
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    Topics: Geography , Economics
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    The New Geography of Capitalism: Firms, Finance, and Society (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
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    Topics: Geography , Economics
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    Econometric modelling of the regional knowledge production function in Europe (2015)
    Oxford University Press
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    Publication Date: 2015-10-14
    Description: By adopting a semiparametric approach, the ‘traditional’ regional knowledge production function is developed in three complementary directions. First, the model is augmented with region-specific time trends to account for endogeneity due to selection on unobservables . Second, the nonparametric part of the model relaxes the standard assumptions of linearity and additivity regarding the effect of R&D and human capital. Finally, the assumption of homogeneity in the effects of R&D and human capital is also relaxed by explicitly accounting for the differences between developed and lagging regions. The analysis of the genesis of innovation in the regions of the European Union unveils nonlinearities, threshold effects, complex interactions and shadow effects that cannot be uncovered by standard parametric formulations.
    Keywords: C14 - Semiparametric and Nonparametric Methods, C23 - Models with Panel Data, O32 - Management of Technological Innovation and R&D, R11 - Regional Economic Activity: Growth, Development, and Changes
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    Topics: Geography , Economics
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    Cover Page (2015)
    Oxford University Press
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    Publication Date: 2015-12-18
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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    Subscription Page (2015)
    Oxford University Press
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    Publication Date: 2015-12-18
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    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
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  • 85
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    Application of strain to orbital-spin-coupled system MnV2O4 at cryogenic temperatures within a transmission electron microscope (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: The impact of mechanical stress on the morphology of crystallographic and magnetic domains in shape-controlled specimens of an orbital-spin-coupled system, MnV 2 O 4 , was examined by cryogenic Lorentz microscopy. Because of the difference in thermal expansion coefficients of MnV 2 O 4 and the supporting Mo mesh, compression on the order of 0.01% was applied to the thin-foil specimens near the structural/magnetic phase transformation temperatures. The extent of compression was comparable to the lattice striction associated with the cubic-to-tetragonal phase transformation in MnV 2 O 4 . The applied strain thus clearly influenced the morphology of crystallographic domains (i.e. twinning configuration in the tetragonal phase) produced during cooling. The magnetic domain structure was entirely dependent on the configuration of twinning in the tetragonal phase. The observations in this study provided useful information for understanding the relationship between the crystallographic domains and the magnetic domains in MnV 2 O 4 .
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    Higher-order assemblies of BAR domain proteins for shaping membranes (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: Most cellular organelles contain lipid bilayer membranes. The earliest characterization of cellular organelles was performed by electron microscopy observation of such membranes. However, the precise mechanisms for shaping the membrane in particular subcellular organelles is poorly understood. Classically, the overall cellular shape, i.e. the shape of the plasma membrane, was thought to be governed by the reorganization of cytoskeletal components such as actin and microtubules. The plasma membrane contains various submicron structures such as clathrin-coated pits, caveolae, filopodia and lamellipodia. These subcellular structures are either invaginations or protrusions and are associated with the cytoskeleton. Therefore, it could be hypothesized that there are membrane-binding proteins that cooperates with cytoskeleton in shaping of plasma membrane organelles. Proteins with the Bin–Amphiphysin–Rvs (BAR) domain connect a variety of membrane shapes to actin filaments. The BAR domains themselves bend the membranes by their rigidity and then mold the membranes into tubules through their assembly as spiral polymers, which are thought to be involved in the various submicron structures. Membrane tubulation by polymeric assembly of the BAR domains is supposed to be regulated by binding proteins, binding lipids and the mechanical properties of the membrane. This review gives an overview of BAR protein assembly, describes the significance of the assembly and discusses how to study the assembly in the context of membrane and cellular morphology. The technical problems encountered in microscopic observation of BAR domain assembly are also discussed.
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    Morphological and chemical analysis of bainite in Cu-17Al-11Mn (at.%) alloys by using orthogonal FIB-SEM and double-EDS STEM (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: In this study, new microscopy techniques were developed for understanding the mechanism for the bainitic transformation in a Cu–17Al–11Mn (at%) alloy. An orthogonally arranged focused ion beam and a scanning electron microscope were employed to observe three-dimensional (3D) morphology of the bainite phase, in addition to compositional analysis by using a scanning transmission electron microscope equipped with a double-detector energy-dispersive X-ray spectrometer system. The 3D morphology of these samples was observed at different aging times and aging temperatures; the results obtained indicated that with increasing aging time and/or aging temperature, the bainite phase at the initial stage of formation exhibits a plate-like shape, which changes to a lenticular form. A habit plane was uniquely determined as ~{9 3 2} by the combination of 3D image reconstruction and an electron back-scattered diffraction technique. The compositional analysis revealed the spatial distribution of the compositional variation between the bainite and matrix phases in the initial stages of the transformation. In the bainite phase, the Cu concentration was higher, while the concentrations of Al and Mn were lower than those in the surrounding matrix, indicative of the diffusion of the constituent elements with the growth of the bainite phase.
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    Cofilin contributes to phagocytosis of IgG-opsonized particles but not non-opsonized particles in RAW264 macrophages (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: Cofilin is an actin-binding protein that severs actin filaments. It plays a key role in regulating actin cytoskeletal remodeling, thereby contributing to diverse cellular functions. However, the involvement of cofilin in phagocytosis remains to be elucidated. We examined the spatiotemporal localization of cofilin during phagocytosis of IgG-opsonized erythrocytes, IgG-opsonized latex beads and non-opsonized latex beads. Live-cell imaging showed that GFP-cofilin accumulates in the sites of IgG-opsonized particle binding and in phagocytic cups. Moreover, immunofluorescence microscopy revealed that endogenous cofilin localizes to phagocytic cups engulfing IgG-opsonized particles, but not non-opsonized latex beads. Scanning electron microscopy demonstrated a notable difference in morphology between phagocytic structures in IgG-dependent and IgG-independent phagocytosis. In phagocytosis of IgG-opsonized particles, sheet-like pseudopodia extended along the surface of IgG-opsonized particles to form phagocytic cups. In contrast, in opsonin-independent phagocytosis, long finger-like filopodia captured non-opsonized latex beads. Importantly, non-opsonized beads sank into the cells without extending phagocytic cups. Our analysis of cofilin mutant expression demonstrates that phagocytosis of IgG-opsonized particles is enhanced in cells expressing wild-type cofilin or active mutant cofilin-S3A, whereas the uptake of non-opsonized latex beads is not. These data suggest that cofilin promotes actin cytoskeletal remodeling to form phagocytic cups by accelerating actin turnover and thereby facilitating phagosome formation. In contrast, cofilin is not involved in opsonin-independent phagocytosis of latex beads.
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    Cover Page (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
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  • 90
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    Quantitative analysis of cation mixing and local valence states in LiNixMn2-xO4 using concurrent HARECXS and HARECES measurements (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: Cation mixing in positive electrode materials for rechargeable lithium ion batteries, LiNi x Mn 2– x O 4 ( x = 0, 0.2, 0.5) and Li 0.21 Ni 0.7 Mn 1.64 O 4– (denoted as x = 0.7), is analyzed by high-angular-resolution electron-channeling X-ray/electron spectroscopy (HARECXS/HARECES) techniques, using energy-dispersive X-ray spectroscopy and electron energy-loss spectroscopy. Mixing between the tetrahedral lithium sites and the octahedral transition metal sites is quantified, and the site-dependent valence states of the transition metals are examined. In the non-doped ( x = 0) sample, Mn was found to occupy only octahedral sites as either Mn 3+ or Mn 4+ . For x = 0.2–0.7, some of the nickel ions (6–13% depending on x ) occupy tetrahedral anti-sites. All the nickel ions are in the divalent state, regardless of the occupation site. For x = 0.2 and 0.7, manganese ions occupy both octahedral and tetrahedral sites; those in the octahedral sites are tetravalent, while the tetrahedral sites contain a mixture of divalent and trivalent ions. For x = 0.5, manganese occupies only the octahedral sites, with all ions determined to be in the tetravalent state (within experimental accuracy). All the samples substantially satisfied the local charge neutrality conditions. This study demonstrates the feasibility of using HARECXS/HARECES for quantitative analysis of the atomic configuration and valence states in lithium manganese oxide spinel materials.
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    Application of low vacuum scanning electron microscopy for Papanicolaou-stained slides for cytopathology examinations (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: Papanicolaou (Pap)-stained slides are usually observed using a transmitted light microscope for cytopathology. However, progress in pathological examinations has created a need for new diagnostic tools, because cytopathological preparations do not allow additional examinations without a loss of specimen, unlike histopathology. Low-vacuum scanning electron microscopy (LVSEM) can reveal the surface topography at an ultrastructual resolution without metal coating. The aim of this study was to determine the conditions required for observing Pap-stained slides of oral smears using LVSEM without any loss of specimen and to reexamine the same slides again using light microscopy, while preserving the cytopathological information.
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    Motion analysis and ultrastructural study of a colonial diatom, Bacillaria paxillifer (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: The pennate diatom, Bacillaria paxillifer , forms a colony in which adjacent cells glide smoothly and almost continuously, yet no obvious apparatus driving the movement, such as flagella or cilia, is observed. Thus far, neither the mechanism nor physiological significance of this movement has been well understood. Here, we report quantitative analysis of the gliding motion of B. paxillifer and morphological analysis of this diatom with light and electron microscopes. The gliding of pairs of adjacent B. paxillifer cells in a colony was cyclic with rather constant periods while the average gliding period varied from a few seconds to multiples of 10 s among colonies. The gliding was compromised reversibly by inhibitors for actin and myosin, suggesting involvement of the actomyosin system. Indeed, we observed two closely apposed actin bundles near the raphe by fluorescence-labeled phalloidin staining. Using electron microscopy, we observed filamentous structures that resemble the actin bundles seen with fluorescence microscopy, and we also found novel electron-dense structures located between the plasma membrane and these actin-like filaments. From these and other observations, we suggest that B. paxillifer also uses actin bundles and propose a putative myosin as a molecular motor in the gliding of unicellular diatoms.
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    Subscription Page (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
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    Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes (2016)
    Oxford University Press
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    Publication Date: 2016-05-29
    Description: Mitochondrial cytochrome c oxidase utilizes electrons provided by c ytochrome c for the active vectorial transport of protons across the inner mitochondrial membrane through the reduction of molecular oxygen to water. Direct structural evidence on the transient cytochrome c oxidase–cytochrome c complex thus far, however, remains elusive and its physiological relevant oligomeric form is unclear. Here, we report on the 2D crystallization of monomeric bovine cytochrome c oxidase with tightly bound cytochrome c at a molar ratio of 1:1 in reconstituted lipid membranes at the basic pH of 8.5 and low ionic strength.
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    Local variety and firm diversification: an evolutionary economic geography perspective (2016)
    Oxford University Press
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    Publication Date: 2016-09-20
    Description: The aim of this article is to investigate whether and how local agglomeration forces—related and unrelated variety—influence firm diversification. Using a large dataset of 5112 Italian manufacturing business groups for the year 2001, and estimating Tobit models, we show the ‘consistency’ between the patterns of firm diversification and that of the local system in which the firm is located. Specifically, firms located in local systems dominated by unrelated variety are more likely to show unrelated diversification patterns, while firms located in local systems dominated by related variety are more likely to show related diversification patterns. This supports the Evolutionary Economic Geography prediction of firm similarity ‘within’ the same local system, and firm heterogeneity ‘between’ different local systems.
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    Topics: Geography , Economics
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    Globalization and local profiles of economic growth and industrial change (2016)
    Oxford University Press
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    Publication Date: 2016-09-20
    Description: The aggregate German economy is characterized by a secular decline of manufacturing and a rise of modern service industries. This trend of structural change is not uniform across space, however. Some regions exhibit it at an accelerated pace, while other regions reinforced their manufacturing specializations. We first categorize all German regions into one of three groups, with ‘pro-trend’, ‘anti-trend’ or ‘featureless’ growth and provide a detailed comparison of these groups. Afterwards we propose an explanation why a particular region ended up in one of those groups: We argue that the profiles of regional growth and change are systematically related to the initial sizes, and the import and export exposures of the local manufacturing sectors.
    Keywords: F16 - Trade and Labor Market Interactions, O14 - Industrialization ; Manufacturing and Service Industries ; Choice of Technology, R11 - Regional Economic Activity: Growth, Development, and Changes
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    Topics: Geography , Economics
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    Direct observation of the actin filament by tip-scan atomic force microscopy (2016)
    Oxford University Press
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    Publication Date: 2016-09-03
    Description: Actin filaments, the actin–myosin complex and the actin–tropomyosin complex were observed by a tip-scan atomic force microscope (AFM), which was recently developed by Olympus as the AFM part of a correlative microscope. This newly developed AFM uses cantilevers of similar size as stage-scan AFMs to improve substantially the spatial and temporal resolution. Such an approach has previously never been possible by a tip-scan system, in which a cantilever moves in the x , y and z directions. We evaluated the performance of this developed tip-scan AFM by observing the molecular structure of actin filaments and the actin–tropomyosin complex. In the image of the actin filament, the molecular interval of the actin subunits (~5.5 nm) was clearly observed as stripes. From the shape of the stripes, the polarity of the actin filament was directly determined and the results were consistent with the polarity determined by myosin binding. In the image of the actin–tropomyosin complex, each tropomyosin molecule (~2 nm in diameter) on the actin filament was directly observed without averaging images of different molecules. Each tropomyosin molecule on the actin filament has never been directly observed by AFM or electron microscopy. Thus, our developed tip-scan AFM offers significant potential in observing purified proteins and cellular structures at nanometer resolution. Current results represent an important step in the development of a new correlative microscope to observe nm-order structures at an acceptable frame rate (~10 s/frame) by AFM at the position indicated by the fluorescent dye observed under a light microscope.
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    Precision enhancement in boundary element methods with application to electron optics (2016)
    Oxford University Press
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    Publication Date: 2016-09-03
    Description: A hybrid approach is presented for obtaining electric potentials for use in electron optics modeling. An initial solution from the boundary element method (BEM) is used to derive the bounding potential of a cylindrical subdomain subsequently used in a Fourier series solution. The approach combines the inherent precision of this analytic solution with the flexibility of BEM to describe practical, non-idealized systems of electrodes. The resulting lens field in the Fourier series subdomain is of higher precision, thereby allowing smaller errors in subsequent calculations of electron ray paths. The effects of aberrations are thus easier to observe in tracing non-paraxial rays. Example ray-traces through a simple, known einzel lens are given as validation of this approach.
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    A spider toxin, {omega}-agatoxin IV A, binds to fixed as well as living tissues: cytochemical visualization of P/Q-type calcium channels (2016)
    Oxford University Press
    Details
    Publication Date: 2016-09-03
    Description: -Agatoxin IV A, a peptidyl toxin from Agelenopsis aperta venom, selectively binds to voltage-gated P/Q-type calcium channels. -Agatoxin IV A has been used as a selective tool in pharmacological and electrophysiological studies. Visualization of P/Q-type calcium channels has previously been accomplished using biotin-conjugated -Agatoxin IV A in freshly prepared mouse cerebellar and hippocampal slices (Nakanishi et al ., J. Neurosci. Res. , 41 , 532, 1995). Here biotinylated -agatoxin IV A was applied to transcardially fixed brain slices prepared with various fixatives. -Agatoxin IV A did not bind to fixed tissues from P/Q-type calcium channel knockout mice, confirming that binding to normal, fixed tissues was not an artifact. Using transmission electron microscopy, locations of biotinylated -agatoxin IV A binding sites visualized with gold-conjugated streptavidin showed a similar pattern to those visualized with antibody. The ability of biotinylated -agatoxin IV A to bind to fixed tissue provides a new cytochemical technique to study molecular architecture of synapses.
    Print ISSN: 0022-0744
    Electronic ISSN: 1477-9986
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Published by Oxford University Press on behalf of The Japanese Electron Microscopy Society.
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    High-efficiency antibody discovery achieved with multiplexed microscopy (2016)
    Oxford University Press
    Details
    Publication Date: 2016-09-03
    Description: The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully leveraged by accessing primary B-cell populations from evolutionarily divergent species such as chickens.
    Print ISSN: 0022-0744
    Electronic ISSN: 1477-9986
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Published by Oxford University Press on behalf of The Japanese Electron Microscopy Society.
    Location Call Number Expected Availability
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    PAPER CURRENT
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