ALBERT

Beschreibung: Ghana is urbanising rapidly, and over half of the country’s population have lived in urban areas since 2010. Although research has proliferated to explore Ghana’s urbanisation, there is a dearth of research that holistically explores the wider sustainability implications of urbanisation, offers comparative perspectives in the context of large and smaller urban areas, and provides a perspective of local level urbanisation in the context of resource extraction (mining). This study comparatively assesses two urban areas in Ghana (Kumasi and Obuasi), by conducting a spatio-temporal analysis of land cover change through remote sensing and by analysing demographic change through a synthesis of published population data, in order to highlight the sustainability implications of urbanisation. The results show that urbanisation has been rapid, and has resulted in changes in land cover and demography in Kumasi and Obuasi. The sustainability implications of urbanisation are identified to include limited economic opportunities, socio-spatial segregation, and destruction of natural vegetation. The evidence in this study provides insights into urbanisation in Ghana, and suggests that the positive sustainability impacts of urbanisation may be eroded by how factors such as market forces and land tenure interact at the local level.

Beschreibung: The optimization of ecological resource allocation is increasingly seen as a potential solution for urban revitalization and sustainable land use planning, and the key point is to assess and simulate the spatial arrangement of the ecological land. In this study, we proposed a conceptual framework with the aim of reoccupying ecological resources for rust belt cities from the perspective of eco-economic trade-offs. The ecological security pattern, the urban development pattern, and the ecological quality of cropland were constructed and evaluated to measure the development level of an ecological system and a socio-economic system. Furthermore, the results were used as the constraints that influenced land use distribution to simulate the ecological land reoccupation pattern. The suitable area, the preservation area, the configurable area, and the unsuitable area in the reoccupation pattern accounted for 6.94%, 49.97%, 28.17%, and 0.69%, respectively. Significantly, under strict cropland protection policies, the available space for ecological land expansion was heavily compressed. Therefore, the emphasis on agricultural production should be reexamined to release more space for ecological resources. This method could be an effective pathway to alleviate the pressures on urban and natural space caused by the competition between land-use activities, such as economic development, agricultural production, and ecological conservation. The findings are expected to promote urban revitalization, green agriculture, and sustainable social development in rust belt cities, and provide certain references for the utilization of land resources and regional policy making.

Beschreibung: As acidic deposition has decreased across Eastern North America, forest soils at some sites are beginning to show reversal of soil acidification. However, the degree of recovery appears to vary and is not fully explained by deposition declines alone. To assess if other site and soil factors can help to explain degree of recovery from acid deposition, soil resampling chemistry data (8- to 24-year time interval) from 23 sites in the United States and Canada, located across 25° longitude from Eastern Maine to Western Ontario, were explored. Site and soil factors included recovery years, sulfate (SO42−) deposition history, SO42− reduction rate, C horizon pH and exchangeable calcium (Ca), O and B horizon pH, base saturation, and exchangeable Ca and aluminum (Al) at the time of the initial sampling. We found that O and B horizons that were initially acidified to a greater degree showed greater recovery and B horizon recovery was further associated with an increase in recovery years and lower initial SO42− deposition. Forest soils that seemingly have low buffering capacity and a reduced potential for recovery have the resilience to recover from the effects of previous high levels of acidic deposition. This suggests, that predictions of where forest soils acidification reversal will occur across the landscape should be refined to acknowledge the importance of upper soil profile horizon chemistry rather than the more traditional approach using only parent material characteristics.

Beschreibung: The genus Thujopsis (Cupressaceae) comprises monoecious coniferous trees endemic to Japan. This genus includes two varieties: Thujopsis dolabrata (L.f.) Siebold et Zucc. var. dolabrata (southern variety, Td) and Thujopsis dolabrata (L.f.) Siebold et Zucc. var. hondae Makino (northern variety, Th). The aim of this study is to understand the phylogeographic and genetic population relationships of the genus Thujopsis for the conservation of genetic resources and future breeding. A total of 609 trees from 22 populations were sampled, including six populations from the Td distribution range and 16 populations from the Th distribution range. The genotyping results for 19 expressed sequence tag (EST)-based simple sequence repeat (SSR) markers, followed by a structure analysis, neighbor-joining tree creation, an analysis of molecular variance (AMOVA), and hierarchical F statistics, supported the existence of two genetic clusters related to the distribution regions of the Td and Th varieties. The two variants, Td and Th, could be defined by their provenance, in spite of the ambiguous morphological differences between the varieties. The distribution ranges of both variants, which have been defined from their morphology, was confirmed by genetic analysis. The Th populations exhibited relatively uniform genetic diversity, most likely because Th refugia in the glacial period were scattered throughout their current distribution area. On the other hand, there was a tendency for Td’s genetic diversity to decrease from central to southern Honshu island. Notably, the structure analysis and neighbor-joining tree suggest the hybridization of the two varieties in the contact zone. More detailed studies of the genetic structure of Td are required in future analyses.

Beschreibung: In agricultural science, the establishment of a given cropping system in mountainous areas is often understood from the relationship between differences of altitude-specific, agroecological conditions and crop cultivation characteristics. However, social factors can also play a role. We aimed to clarify how the cropping system is maintained through examining sociocultural factors, specifically land tenure and marriage systems, in an agricultural community in rural mountainous Africa. Several surveys based on participatory observation accompanied by home stays were conducted to determine people who participated in cropping systems and to understand which social system maintained the cropping system. We found that around 70% of target households in Kiboguwa village cultivated three staple crops (maize, cassava and rice) using the same cropping system and almost no farmers outside the village used the village’s sloped fields, meaning that the villagers maintained the cropping system. Households acquired nearby sloped fields by various means such as inheriting land through maternal lineage of household heads or wives. We observed virilocal and uxorilocal residence at similar degrees—and if either the husband or wife was from outside the village, that household would also have fields outside the village. However, nearly 80% of marriages were intravillage and villagers predominantly used fields located within the village limits regardless of the residence type, which helped maintain the cropping system.

Beschreibung: The research presented in the paper intends to overcome an information gap on the evolution of urbanized surfaces in Italy which in the studies carried out so far have never been available. The only historical data on this form of land use date back to the 1950s, and were extracted from a national cartography created by the Military Geographic Institute. The next chronological section available was then that of the noughties, already digital. However, much more frequent data were processed by the ten-year censuses by the National Institute of Statistics, but concerning buildings and not urbanized areas. By processing building census data, this study has put together some novel information on land take dynamics between the end of World War II and the year 2000, highlighting the more intensive processes that occurred at an extraordinary rate in the ‘70′s and ‘80′s, obtaining unprecedented information on the speed of transformation of the territory in these decades of economic boom. Through this method, we were able to obtain numerous geographical indications previously lacking on a national scale, highlight the yet significant vigor of this phenomenon and develop an inferential scenario.

Beschreibung: Access and use of seed of improved varieties of groundnut among farmers can improve farmers’ livelihoods and contribute to the potential of crop production in Tanzania. This paper analyzes factors underpinning the adoption of improved groundnut varieties among farmers to pave the way for upscaling quality seed used for increased production and commodity business in farming communities. A four-stage stratified sampling was used to collect data from 300 groundnut farmers in seven agro-ecological zones through individual interviews. Secondary data were collected from the literature and the Tanzania Agricultural Research Institute at Naliendele centre (TARI–Naliendele). Descriptive statistics and Probit regression model were used for data analysis. The empirical results showed that Johari 1985, Pendo 1998, Naliendele 2009, Mnanje 2009, Mangaka 2009 and Nachi 2015, are the main six improved groundnut varieties used by farmers, with Pendo 1998 having the highest adoption rate (17.1%). In the grain market, four varieties, namely Pendo 1998, Mnanje 2009, Nachi 2015 and Johari 1985, were observed to be highly preferred by grain off-takers. Furthermore, among the adopted improved varieties, Nachi 2015, is observed to be the most consistent high yielding variety, ranging from 1100 kg/ha to 1500 kg/ha in all agro-ecological zones. A farmer’s decision to adopt new varieties is affected by age and gender, farmer group membership, availability of improved seed and seed cost. Overall, male farmers are more likely to adopt improved varieties of groundnut than female farmers. The implications of these findings are also discussed, in particular in the area of policy support.

Beschreibung: The convenient model Arabidopsis thaliana has allowed tremendous advances in plant genetics and physiology, in spite of only being a weed. It has also unveiled the main molecular networks governing, among others, abiotic stress responses. Through the use of the latest genomic tools, Arabidopsis research is nowadays being translated to agronomically interesting crop models such as tomato, but at a lagging pace. Knowledge transfer has been hindered by invariable differences in plant architecture and behaviour, as well as the divergent direct objectives of research in Arabidopsis vs. crops compromise transferability. In this sense, phenotype translation is still a very complex matter. Here, we point out the challenges of “translational phenotyping” in the case study of drought stress phenotyping in Arabidopsis and tomato. After briefly defining and describing drought stress and survival strategies, we compare drought stress protocols and phenotyping techniques most commonly used in the two species, and discuss their potential to gain insights, which are truly transferable between species. This review is intended to be a starting point for discussion about translational phenotyping approaches among plant scientists, and provides a useful compendium of methods and techniques used in modern phenotyping for this specific plant pair as a case study.

Beschreibung: This study presents an integrated examination of both the ecosystem services (ES) and ecosystem disservices (EDS) associated with smallholder animal husbandry in rural livelihoods in three villages in southeast South Africa. It recognises the contribution of ES supporting and resulting from smallholder livestock and poultry production, but also details the limiting factors or EDS, such as tick-borne disease, birds of prey or unpalatable rangeland, produced by the same system. Using a mixed-methods approach, including focus group discussions with various Participatory Learning and Action (PLA) activities, key informant interviews, household surveys and land-use change mapping on GIS, we consider the relative value and benefits from ES after the effects of EDS, as well as the management and strategies that households adopt to minimise EDS. The effects of ES and EDS were expressed in economic terms to provide a common framework to assess the magnitude of their contribution or effect. Although animal husbandry made measurable contributions to households, with an average gross value of between R2605–R9753 across villages, EDS undermined the meaningful production of livestock and poultry goods and services. The average EDS-induced economic loss in households was between R8289–R22,426 per annum. Despite active management, often at substantial cost to the household, EDS undermined the health of livestock which resulted in a loss of the animal or potential goods and services produced. We emphasise the need to recognise both the positive and negative contributions of ecosystems to identify the complex feedbacks between ES and EDS which interact to determine local ways of doing things.

Beschreibung: Species with wide geographical ranges exhibit specific adaptations to local climates, which may result in diverging responses among populations to changing conditions. Climate change has advanced spring phenology worldwide, but questions of whether and how the phenological responses to warming differ among individuals across the natural range of a species remain. We conducted two experiments in January and April 2019, and performed daily observations of the timings of bud break in 1-year-old seedlings of sugar maple (Acer saccharum Marshall) from 25 Canadian provenances at two thermal conditions (14/10 and 18/14 °C day/night temperature) in a controlled environment. Overall, bud break started 6 days from the beginning of the experiments and finished after 125 days. The earlier events were observed in seedlings originating from the colder sites. Bud break was delayed by 4.8 days per additional degree Celsius in the mean annual temperature at the origin site. Warming advanced the timing of bud break by 17–27 days in January and by 3–8 days in April. Similar advancements in bud break were observed among provenances under warming conditions, which rejected our hypothesis that sugar maple populations have different phenological responses to warming. Our findings confirm the differentiation in ecotypes for the process of bud break in sugar maple. In cases of homogenous spring warming across the native range of sugar maple, similar advancements in bud phenology can be expected in different populations.

Beschreibung: This case study from Stockholm County, Sweden, explores practitioners’ experiences of barriers and bridges in municipal planning practices to support actions for ecosystem services. This qualitative study is based on information gathered from a focus group, workshops, and semi-structured interviews, which aided in identifying key factors for integrating ecosystem services in municipal planning. We identified 10 key factors divided into three themes: (i) regulatory framework and political support, (ii) local organizational capacity, and (iii) local adaptation of tools and practices. In particular, the practitioners pointed to the need for the development of legal support and regulations for ecosystem services on the national and EU policy levels. Furthermore, the need for local capacity building and understanding of ecosystem services as well as increased regional support to enhance local knowledge exchange and learning was emphasized. Also, in a decentralized local governance system such as in Sweden, to fully implement ecosystem services in urban planning for sustainable development, locally adapted practical tools and monitoring procedures were considered important.

Beschreibung: Sugarcane is an important industrial crop because it is the major source of white sugar. It is also one of the crops for the alcohol and biofuel industries. Disease-causing organisms can significantly decrease the productivity of sugarcane plants and sugar quality. Among the disease-causing organisms, Colletotrichum falcatum Went causes the most significant economic loss (5–50%) in the sugarcane production due to red rot disease. This loss results in only 31% sugar recovery. It is reported that C. falcatum can kill sugarcane plants. Currently, there is no sustainable way of preventing red rot disease from spreading in sugarcane plantations. Many popular sugarcane varieties are no longer used in sugarcane cultivation because of their susceptibility to C. falcatum. The objectives of this manuscript were to: (i) summarize existing approaches for the early detection of red rot disease and controlling techniques of red rot disease in the field and laboratory and (ii) assess red rot disease control effectiveness so as to propose better methods for mitigating the spread C. falcatum. If our proposition is adopted or practiced, it could significantly contribute to the mitigation of C. falcatum infection in the sugarcane industry. This could enable achieving sustainable cultivation of sugarcanes to guarantee the sustainability of the sugar industry in the tropics and the subtropics.

Beschreibung: The transition from intensive conventional agriculture to sustainable agriculture has become a global priority. This is due to the need for environmentally friendly agriculture to ensure sufficient food for a rapidly growing population. The bioeconomy is essential to progress in the field of sustainable agriculture. It contributes to the conservation of biological resources through circular and comprehensive management. The bioeconomy prioritizes the reduction and reuse of materials and products. The focus of this study is the use of biodegradable/compostable raffia in protected horticultural crops in the Province of Almería (Spain). The analysis and evaluations, based on an extensive literature review and information given by stakeholders, determined that biodegradable raffia use significantly improves the management of residual biomass in Almería. However, biodegradable raffia is only used in a small percentage of crops even though it is a sustainable alternative and profitable for farmers. The economic analysis incorporates the higher cost of recycling non-biodegradable raffia.

Beschreibung: Sustainable agricultural production is endangered by several ecological factors, such as drought, extreme temperatures, excessive salts, parasitic ailments, and insect pest infestation. These challenging environmental factors may have adverse effects on future agriculture production in many countries. In modern agriculture, conventional crop-breeding techniques alone are inadequate for achieving the increasing population’s food demand on a sustainable basis. The advancement of molecular genetics and related technologies are promising tools for the selection of new crop species. Gene pyramiding through marker-assisted selection (MAS) and other techniques have accelerated the development of durable resistant/tolerant lines with high accuracy in the shortest period of time for agricultural sustainability. Gene stacking has not been fully utilized for biotic stress resistance development and quality improvement in most of the major cultivated crops. This review emphasizes on gene pyramiding techniques that are being successfully deployed in modern agriculture for improving crop tolerance to biotic and abiotic stresses for sustainable crop improvement.

Beschreibung: Research Highlights: The informed decisions on land use require assessment of the impacts of these decisions on the supply of different ecosystem services. Background and Objectives: Scenario modeling can be used to provide harmonized and quantitative information on the impacts of various zoning decisions on the provision of various ecosystem services. In this study, we explored the effects of land-use zoning decisions on the provision of roundwood and energy wood, carbon stock of living tree components, berry yields, scenic beauty and recreation. Materials and Methods: Three alternative forest scenarios were formulated in terms of restrictions on forest management for already established land-use zones by the provinces in Finland. These data were integrated with the National Forest Inventory (NFI) plot data for forest dynamics modeling. Results: In Finland, 9% of the forest land was protected and 9% was under restricted forest management due to legislative decisions, forest owners’ decisions, or regional land use plans in 2016. These established zoning decisions resulted in an estimated 17% smaller roundwood and energy wood removals per year in 2016–2025 compared to the estimated wood removals in the scenario without any restrictions. The decrease in annual gross stumpage earnings was EUR 775 million per year. The carbon stock of living tree components in 2025 was 12% larger than in the scenario without any restrictions. Bilberry yield increased due to the restrictions considered in our study, while the restrictions had only a slight effect on cowberry yield at province and country levels. The restrictions increased the recreation value, particularly in Lapland, but only slightly affected scenic beauty at province and country levels. Conclusions: Scenario analyses support informed decision making and the balancing of different forest uses. Harmonized translation of restrictions and the use of standard indicators to model the impacts also support comparisons between the regions.

Beschreibung: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an RNA virus, is responsible for the coronavirus disease 2019 (COVID-19) pandemic of 2020. Experimental evidence suggests that microRNA can mediate an intracellular defence mechanism against some RNA viruses. The purpose of this study was to identify microRNA with predicted binding sites in the SARS-CoV-2 genome, compare these to their microRNA expression profiles in lung epithelial tissue and make inference towards possible roles for microRNA in mitigating coronavirus infection. We hypothesize that high expression of specific coronavirus-targeting microRNA in lung epithelia may protect against infection and viral propagation, conversely, low expression may confer susceptibility to infection. We have identified 128 human microRNA with potential to target the SARS-CoV-2 genome, most of which have very low expression in lung epithelia. Six of these 128 microRNA are differentially expressed upon in vitro infection of SARS-CoV-2. Additionally, 28 microRNA also target the SARS-CoV genome while 23 microRNA target the MERS-CoV genome. We also found that a number of microRNA are commonly identified in two other studies. Further research into identifying bona fide coronavirus targeting microRNA will be useful in understanding the importance of microRNA as a cellular defence mechanism against pathogenic coronavirus infections.

Beschreibung: In this study, dynamic hardness tests on solid and engineered wood flooring specimens of Eucalyptus globulus Labill. and Eucalyptus grandis W. Hill ex Maiden hardwoods were performed because nowadays, these fast-growing hardwoods are still scarcely employed for this use. Furthermore, another two examples of hardwood commonly applied on wood flooring, Quercus robur L. and Hymenaea courbaril L., were also tested. To compare their properties, a dynamic impact hardness test based on the impact of steel balls, with several diameters, and drop heights was developed. Accordingly, 120 solid wood flooring specimens and 120 engineering wood flooring specimens were producing with these four hardwood species. Dynamic impact tests were made with three steel balls of different diameters (30–40–50 mm), and they were carried out from five different drop heights (0.60–0.75–0.90–1.05–1.20 m). The impact of the steel ball drew the size of the footprint on the surface and this mark was measured with a digital caliper for both dimensions, diameter and depth, as footprint diameter (FD) and indentation depth (ID). Data from 3000 samples, corresponding to 120 different individual groups (4 species × 3 ball diameters × 5 drop height × 2 floor type) were analyzed. Results indicated that the variability of ID (CV between 19.25–25.61%) is much greater than the values achieved for FD (CV between 6.72–7.91%). Regarding the fast-growing hardwood species tested, E. globulus showed a similar behavior to traditional hardwood applied on wood flooring in Europe, Q. robur, and it could be a promising growth in the flooring industry. However, E. grandis showed the worst values compared to traditional hardwood in all test configurations.

Beschreibung: The VCAN/versican gene encodes an important component of the extracellular matrix, the chondroitin sulfate proteoglycan 2 (CSPG2/versican). Heterozygous variants targeting exon 8 of VCAN have been shown to cause Wagner disease, a rare autosomal dominant non-syndromic vitreoretinopathy that induces retinal detachment, cataracts and permanent visual loss. In this study, we report on six patients from three unrelated families with Wagner disease in whom we identified three novel copy number variations of VCAN. Quantitative real-time polymerase chain reaction analysis identified deletions, including one exon–intron boundary of exon 8 or both exons 8 and 9, causing the haploinsufficiency of VCAN mRNAs.

Beschreibung: Streptococcus salivarius is a significant contributor to the human oral, pharyngeal and gut microbiomes that contribute to the maintenance of health. The high genomic diversity observed in this species is mainly caused by horizontal gene transfer. This work aimed to evaluate the contribution of integrative and conjugative elements (ICEs) and integrative and mobilizable elements (IMEs) in S. salivarius genome diversity. For this purpose, we performed an in-depth analysis of 75 genomes of S. salivarius and searched for signature genes of conjugative and mobilizable elements. This analysis led to the retrieval of 69 ICEs, 165 IMEs and many decayed elements showing their high prevalence in S. salivarius genomes. The identification of almost all ICE and IME boundaries allowed the identification of the genes in which these elements are inserted. Furthermore, the exhaustive analysis of the adaptation genes carried by these elements showed that they encode numerous functions such as resistance to stress, to antibiotics or to toxic compounds, and numerous enzymes involved in diverse cellular metabolic pathways. These data support the idea that not only ICEs but also IMEs and decayed elements play an important role in S. salivarius adaptation to the environment.

Beschreibung: Stearoyl-CoA desaturase (SCD) is known to be an important rate-limiting enzyme in the production of monounsaturated fatty acids (MUFAs). However, the role of this enzyme in goose follicular development is poorly understood. To investigate the metabolic mechanism of SCD during goose follicular development, we observed its expression patterns in vivo and in vitro using quantitative reverse-transcription (qRT)-PCR. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine a cellular model of SCD function in granulosa cells (GCs) via SCD overexpression and knockdown. qRT-PCR analysis showed that SCD was abundantly expressed in the GC layer, and was upregulated in preovulatory follicles. Peak expression was found in F1 and prehierarchal follicles with diameters of 4–6 mm and 8–10 mm, respectively. We further found that mRNA expression and corresponding enzyme activity occur in a time-dependent oscillation pattern in vitro, beginning on the first day of GC culture. By LC-MS/MS, we identified numerous changes in metabolite activation and developed an overview of multiple metabolic pathways, 10 of which were associated with lipid metabolism and enriched in both the overexpressed and knockdown groups. Finally, we confirmed cholesterol and pantothenol or pantothenate as potential metabolite biomarkers to study SCD-related lipid metabolism in goose GCs.

Beschreibung: The aim of the study was to evaluate the changes in glomalin-related soil proteins (GRSP) content, microbial diversity and soil physical quality depending on the type of soil measures of soil improvement and changes in soil health. The study was based on a 100-year stationary field microplot experiment where the soil profiles were collected with preserving the natural soil horizons. The microplot experiment was carried out on eight different soil types: Brunic Arenosol (Dystric I), Rendzic Leptosol, Fluvic Cambisol, Haplic Cambisol (Eutric), Gleyic Phaeozem, Brunic Arenosol (Dystric II), Haplic Cambisol (Eutric II) and Haplic Cambisol (Dystric). These soils are the most common types of agricultural soils in Poland. Relatively significant correlations with the soil quality, physical parameters and the glomalin-related soil proteins have been found. The study determined the total GRSP (T-GRSP) and easily extractable GRSP (EE-GRSP) levels in soils as well as the soil physical quality index and soil’s microbial biodiversity. The GRSP depended on the type of soil and correlated with S-Index and also was responsible for the unique chemical and physical properties of soils. Soils characterized by the highest T-GRSP content belonged to the group of very good and good soil physical quality characterized also by high biological activity, for which there were strong correlations with such parameters as dehydrogenase activity (DHA), microbial biomass content (MBC), microbial nitrogen content (MBN) and total bacteria number (B). The highest T-GRSP content and higher microbial diversity were found in Gleyic Phaeozem, Rendzic Leptosol and Fluvic Cambisol. The T-GRSP and EE-GRSP content were additionally correlated with the number of AMF spores. Very poor and poor soil physical quality according to S-Index characterized Brunic Arenosol (Dystric I) and Haplic Cambisol (Dystric). This research indicates that a specific edaphone of soil microorganisms and GRSP content may be of great importance when assessing a soil’s quality and improvements in soil health. The abundance of glomalin-producing fungi significantly affects the quality of the soil. This effect is particularly important for agricultural soils are threatened by ongoing land degradation.

Beschreibung: Water mites represent the most diverse and abundant group of Arachnida in freshwater ecosystems, with about 6000 species described; however, it is estimated that this number represents only 30% of the total expected species. Despite having strong biotic interactions with their community and having the potential to be exceptional bioindicators, they are frequently excluded from studies of water quality or ecology, due to actual and perceived difficulties of taxonomic identification in this group. The objective of this study is to use the variations in the sequences of the mitochondrial cytochrome oxidase subunit I (COI), also known as the DNA barcodes region, as a tool to assess the diversity of water mites at 24 sites in the Yucatan Peninsula of Mexico. We found 77 genetic groups or putative species corresponding to 18 genera: Arrenurus, Atractides, Centrolimnesia, Eylais, Geayia, Hydrodroma, Hydryphantes, Hygrobates, Koenikea, Krendowskia, Limnesia, Limnochares, Mamersellides, Mideopsis, Neumania, Piona, Torrenticola, and Unionicola. This was significant, since there are only 35 species described for this region. Furthermore, this molecular information has allowed us to infer that there are characteristic assemblies per site. These data will facilitate the incorporation of water mites in different studies while the curatorial work continues to assign a Linnaean name.

Beschreibung: Urban areas may contain a wide range of potential habitats and environmental gradients and, given the many benefits to human health and well-being, there is a growing interest in maximizing their biodiversity potential. However, the ecological patterns and processes in urban areas are poorly understood. Using a widely applicable ecological survey method, we sampled epiphytic lichen communities, important bioindicators of atmospheric pollution, on host Quercus trees in urban parks of London, UK, to test if common patterns relating to lichen diversity are mirrored in urban green spaces. We found lichen diversity to be dependent on host species identity, and negatively related to local tree crowding. In addition, we found a strong negative effect of tree size on lichen diversity, leaving large trees as unexploited niches. A novel network analysis revealed the presence of only pioneer communities, showing the lichen communities are being held in successional stasis, likely due to the heritage effects of SO2 emissions and current nitrogen pollution and particulate emissions. Our study highlights that jointly assessing species richness, community structure and the successional stage can be key to understanding diversity patterns in urban ecosystems. Subsequently, this may help best determine the optimum conditions that will facilitate biodiversity increase within cities.

Beschreibung: Eucalyptus grandis and E. dunnii have high productive potential in the South of Brazil, Uruguay, and central Argentina. This is based on the similarity of the climate and soil of these areas, which form an eco-region called Campos. However, previous results show that these species have differences in their distribution caused by the prioritization of Uruguayan soils for forestry, explained by the particular conditions of each site. In this study, the site variables (climate, soil, and topography) that better explain the distribution of both species were identified, and prediction models of current and future distribution were adjusted for different climate change scenarios (years 2050 and 2070). The distribution of E. grandis was associated with soil parameters, whereas for E. dunnii a greater effect of the climatic variables was observed. The ensemble biomod2 model was the most precise with regard to predicting the habitat for both species with respect to the simple models evaluated. For E. dunnii, the average values of the AUC, Kappa, and TSS index were 0.98, 0.88, and 0.77, respectively. For E. grandis, their values were 0.97, 0.86, and 0.80, respectively. In the projections of climatic change, the distribution of E. grandis occurrence remains practically unchanged, even in the scenarios of temperature increase. However, current distribution of E. dunnii shows high susceptibility in a scenario of increased temperature, to the point that most of the area currently planted may be at risk. Our results might be useful to political government and foresters for decision making in terms of future planted areas.

Beschreibung: Landscape connectivity is increasingly promoted as a conservation tool to combat the negative effects of habitat loss, fragmentation, and climate change. Given its importance as a key conservation strategy, connectivity science is a rapidly growing discipline. However, most landscape connectivity models consider connectivity for only a single snapshot in time, despite the widespread recognition that landscapes and ecological processes are dynamic. In this paper, we discuss the emergence of dynamic connectivity and the importance of including dynamism in connectivity models and assessments. We outline dynamic processes for both structural and functional connectivity at multiple spatiotemporal scales and provide examples of modeling approaches at each of these scales. We highlight the unique challenges that accompany the adoption of dynamic connectivity for conservation management and planning in the context of traditional conservation prioritization approaches. With the increased availability of time series and species movement data, computational capacity, and an expanding number of empirical examples in the literature, incorporating dynamic processes into connectivity models is more feasible than ever. Here, we articulate how dynamism is an intrinsic component of connectivity and integral to the future of connectivity science.

Beschreibung: Although compacted soil can be recovered through root development of planted seedlings, the relationship between root morphologies and soil physical properties remain unclear. We investigated the impacts of soil compaction on planted hybrid larch F1 (Larix gmelinii var. japonica×L. kaempferi, hereafter F1) seedlings with/without N loading. We assumed that N loading might increase the fine root proportion of F1 seedlings under soil compaction, resulting in less effects of root development on soil recovery. We established experimental site with different levels of soil compaction and N loading, where two-year-old F1 seedlings were planted. We used a hardness change index (HCI) to quantify a degree of soil hardness change at each depth. We evaluated root morphological responses to soil compaction and N loading, focusing on ectomycorrhizal symbiosis. High soil hardness reduced the total dry mass of F1 seedlings by more than 30%. Significant positive correlations were found between HCI and root proportion, which indicated that F1 seedling could enhance soil recovery via root development. The reduction of fine root density and its proportion due to soil compaction was observed, while these responses were contrasting under N loading. Nevertheless, the relationships between HCI and root proportion were not changed by N loading. The relative abundance of the larch-specific ectomycorrhizal fungi under soil compaction was increased by N loading. We concluded that the root development of F1 seedling accelerates soil recovery, where N loading could induce root morphological changes under soil compaction, resulting in the persistent relationship between root development and soil recovery.

Beschreibung: Leaf rust (LR) and stem rust (SR) pose serious challenges to wheat production in Kazakhstan. In recent years, the susceptibility of local wheat cultivars has substantially decreased grain yield and quality. Therefore, local breeding projects must be adjusted toward the improvement of LR and SR disease resistances, including genetic approaches. In this study, a spring wheat segregating population of Pamyati Azieva (PA) × Paragon (Par), consisting of 98 recombinant inbred lines (RILs), was analyzed for the resistance to LR and SR at the seedling and adult plant-growth stages. In total, 24 quantitative trait loci (QTLs) for resistance to rust diseases at the seedling and adult plant stages were identified, including 11 QTLs for LR and 13 QTLs for SR resistances. Fourteen QTLs were in similar locations to QTLs and major genes detected in previous linkage mapping and genome-wide association studies. The remaining 10 QTLs are potentially new genetic factors for LR and SR resistance in wheat. Overall, the QTLs revealed in this study may play an important role in the improvement of wheat resistance to LR and SR per the marker-assisted selection approach.

Beschreibung: Chili pepper (Capsicum spp.) is one of the most economically important horticultural crops in the world; its production for the food and pharmaceutical industries has been increasing worldwide. The economic importance of this crop is due, in part, to the nutraceutical properties derived from its secondary metabolism. Drought is the main environmental factor that affects crop production. Nevertheless, studies involving water deficit have considered short-term responses to sharp water deficit rather than long-term acclimation processes through moderate and gradually increasing water deficits, which omitted the dynamics and profile of the secondary metabolism that are part of the plant’s defence system against this stress factor. The present study aimed to identify the different mechanisms that chili pepper plants use to cope with drought stress using a progressive decrease and increase of water availability, conditions that commonly occur for crops in open fields. Four treatments were applied as follows: gradual water deficit (GWD), initial waterlogging with gradual water deficit (IWGD), sudden water deficit with gradual recovery (SWDR), and no deficit of water (NDW). These conditions should represent a more real situation similar to that faced by plants in the agricultural environment. In order to evaluate the response mechanisms associated with these water deficits, changes in phenological variables, proline accumulation, and the gene expression of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), peroxidase (POD), and superoxide dismutase (SOD) were measured in chili pepper plants growing on land under different irrigation regimes in two contrasting soil types in areas where chili pepper plants are cultivated in central Mexico. The variables evaluated showed a differentiated response of the mechanisms in plants growing under different levels of water deficit. Given the differential response observed for the gene expression and morphological and biochemical variables studied in chili pepper plants against different water regimes, in this work, this may have implications for more efficient use of water in crops with high nutraceutical content, in addition to prospects for using products derived from secondary metabolism in the pharmaceutical industry.

Beschreibung: Climate change has the potential to disrupt ecosystem services and further exacerbate the effects of human activities on natural resources. This has significant implications for educational institutions and the populations they serve. As the current crop of landscape architecture students struggles to define its role within the climate crisis and its related social and political underpinnings, a core mission of colleges and universities moving forward should be to provide students with applied knowledge about how climate change affects the landscape. This goes beyond coursework in climate science or policy; for landscape architecture students to be leaders in the response to climate change, they need applied, practical skills. An ever-growing body of the literature focuses on landscape design strategies for climate change adaptation; however, few frameworks integrate these strategies with the hands-on experience students will need to face real-world challenges after graduation. Educational institutions have the potential to utilize their campuses as demonstration sites for applied ecosystem research programs and actively engage students with the design, implementation, politics, and ongoing stewardship of these landscapes. This paper uses a case study methodology to understand how experiential and public-engaged learning pedagogies contribute to student preparedness to address climate change. It examines three cases of engaged learning at the University of California, Davis campus and attributes their impact to intentional connections with research, to the delegation of responsibility; to the openness of spaces for experimentation, and to self-reflection that connects climate with everyday behavior. By promoting experiential learning programs that require students to actively use their heads and their hands to construct and sustainably manage their own campus landscapes, service-learning studios and internships can provide opportunities for students to address the real scenarios of climate crisis and resilience.

Beschreibung: Marek’s disease (MD) represents a significant global economic and animal welfare issue. Marek’s disease virus (MDV) is a highly contagious oncogenic and highly immune-suppressive α-herpes virus, which infects chickens, causing neurological effects and tumour formation. Though partially controlled by vaccination, MD continues to have a profound impact on animal health and on the poultry industry. Genetic selection provides an alternative and complementary method to vaccination. However, even after years of study, the genetic mechanisms underlying resistance to MDV remain poorly understood. The Major Histocompatability Complex (MHC) is known to play a role in disease resistance, along with a handful of other non-MHC genes. In this study, one of the largest to date, we used a multi-facetted approach to identify quantitative trait locus regions (QTLR) influencing resistance to MDV, including an F6 population from a full-sib advanced intercross line (FSIL) between two elite commercial layer lines differing in resistance to MDV, RNA-seq information from virus challenged chicks, and genome wide association study (GWAS) from multiple commercial lines. Candidate genomic elements residing in the QTLR were further tested for association with offspring mortality in the face of MDV challenge in eight pure lines of elite egg-layer birds. Thirty-eight QTLR were found on 19 chicken chromosomes. Candidate genes, microRNAs, long non-coding RNAs and potentially functional mutations were identified in these regions. Association tests were carried out in 26 of the QTLR, using eight pure lines of elite egg-layer birds. Numerous candidate genomic elements were strongly associated with MD resistance. Genomic regions significantly associated with resistance to MDV were mapped and candidate genes identified. Various QTLR elements were shown to have a strong genetic association with resistance. These results provide a large number of significant targets for mitigating the effects of MDV infection on both poultry health and the economy, whether by means of selective breeding, improved vaccine design, or gene-editing technologies.

Beschreibung: The human microbiome is comprised of the microbes that live on and within an individual, as well as immediately surrounding them. Microbial profiling may have forensic utility in the identification or association of individuals with criminal activities, using microbial signatures derived from a personal microbiome. This review highlights some important aspects of recent studies, many of which have revealed issues involving the effect of contamination of microbial samples from both technical and environmental sources and their impacts on microbiome research and the potential forensic applications of microbial profiling. It is imperative that these challenges be discussed and evaluated within a forensic context to better understand the future directions and potential applications of microbial profiling for human identification. It is necessary that the limitations identified be resolved prior to the adoption of microbial profiling, or, at a minimum, acknowledged by those applying this new approach.

Beschreibung: We evaluated surface-based analysis for assessing the possible relationship between the microstructural properties and particulate matter (i.e., two size fractions of PM2.5 and PM10) adsorption efficiencies of their leaf surfaces on ten woody species. We focused on the effect of PM adsorption capacity between micro-morphological features on leaf surfaces using a scanning electron microscope and a non-contact surface profiler as an example. The species with higher adsorption of PM10 on leaf surfaces were Korean boxwood (Buxus koreana Nakai ex Chung & al.) and evergreen spindle (Euonymus japonicus Thunb.), followed by yulan magnolia (Magnolia denudata Desr.), Japanese yew (Taxus cuspidata Siebold & Zucc.), Japanese horse chestnut (Aesculus turbinata Blume), retusa fringetree (Chionanthus retusus Lindl. & Paxton), maidenhair tree (Ginkgo biloba L.), and royal azalea (Rhododendron schlippenbachii Maxim.). There was a higher capacity for the adsorption of PM2.5 on the leaf surfaces of B. koreana and T. cuspidata, followed by A. turbinata, C. retusus, E. japonicus, G. biloba, and M. denudata. In wax layer tests, T. cuspidata, A. turbinata, R. schlippenbachii, and C. retusus showed a statistically higher PM2.5 capturing capacity than the other species. Different types of trichomes were distributed on the adaxial and abaxial leaves of A. turbinata, C. retusus, M. denudata, pagoda tree (Styphnolobium japonicum (L.) Schott), B. koreana, and R. schlippenbachii; however, these trichomes were absent on both sides of the leaves of G. biloba, tuliptree (Liriodendron tulipifera L.), E. japonicus, and T. cuspidata. Importantly, leaf surfaces of G. biloba and S. japonicum with dense or thick epicuticular leaf waxes and deeper roughness revealed lower PM adsorption. Based on the overall performance of airborne PM capture efficiency, evergreen species such as B. koreana, T. cuspidata, and E. japonicus showed the best results, whereas S. japonicum and L. tulipifera had the lowest capture. In particular, evergreen shrub species showed higher PM2.5 depositions inside the inner wall of stomata or the periphery of guard cells. Therefore, in leaf microstructural factors, stomatal size may be related to notably high PM2.5 holding capacities on leaf surfaces, but stomatal density, trichome density, and roughness had a limited effect on PM adsorption. Finally, our findings indicate that surface-based microstructures are necessarily not a correlation for corresponding estimates with leaf PM adsorption.

Beschreibung: Incidental findings on newborn screening (NBS) are results that are not the target of screening within a given NBS program, but rather are found as a result of the screening and resulting diagnostic workup for that target. These findings may not have an immediate clinical impact on the newborn, but are sometimes an additional benefit of NBS programs and may be considered secondary targets of NBS programs. This work describes four case reports that had incidental findings on the NBS, which eventually led to the diagnosis of another metabolic disease instead of the one that was initially suspected. The first case was a new defect in the cationic amino acid transporter-2 (CAT-2), which was oriented as an arginase-1 deficiency in the newborn. The second case was a maternal glutaric aciduria type 1 (GA-1) that mimicked a carnitine transporter deficiency in the newborn. The third report was a case of lysinuric protein intolerance (LPI), which appeared as high levels of citrulline on the NBS. The fourth case was a mother with homocystinuria that was diagnosed during the biochemical study of vitamin B12 status. All cases provide new or interesting data that will help guide differential diagnosis in the future.

Beschreibung: In domesticated strains of the Nile tilapia, phenotypic sex has been linked to genetic variants on linkage groups 1, 20 and 23. This diversity of sex-loci might reflect a naturally polymorphic sex determination system in Nile tilapia, or it might be an artefact arising from the process of domestication. Here, we searched for sex-determiners in wild populations from Kpandu, Lake Volta (Ghana-West Africa), and from Lake Koka (Ethiopia-East Africa) that have not been subjected to any genetic manipulation. We analysed lab-reared families using double-digest Restriction Associated DNA sequencing (ddRAD) and analysed wild-caught males and females with pooled whole-genome sequencing (WGS). Strong sex-linked signals were found on LG23 in both populations, and sex-linked signals with LG3 were observed in Kpandu samples. WGS uncovered blocks of high sequence coverage, suggesting the presence of B chromosomes. We confirmed the existence of a tandem amh duplication in LG23 in both populations and determined its breakpoints between the oaz1 and dot1l genes. We found two common deletions of ~5 kb in males and confirmed the presence of both amhY and amh∆Y genes. Males from Lake Koka lack both the previously reported 234 bp deletion and the 5 bp frameshift-insertion that creates a premature stop codon in amh∆Y.

Beschreibung: The Brazilian Cerrado biome has undergone major changes, with the incorporation of new areas for agricultural production. While this can certainly provide for the worldwide growing need for agricultural products, especially food, care should be taken to prevent possible environmental degradation. Worldwide, mites of the cohort Gamasina constitute the most abundant and diverse group of soil predatory mites, usually considered important in maintaining the ecological balance of natural environments. Little is known about the abundance and diversity of Gamasina in the Cerrado. The objective of the present work was to evaluate the abundance and diversity of Gamasina in soils of natural vegetation and of agroecosystems in Cerrado areas of the northern Brazilian state of Tocantins. This is considered the first step in the determination of possible role of the local predators as biological control agents, and their potential for practical use locally and elsewhere. Soil samples were taken monthly between July 2015 and June 2016. In total, 1373 Gamasina representing 45 species of 24 genera and 9 families were collected. The most abundant Gamasina belonged to Rhodacaridae in areas of the natural vegetation and to Ascidae in the agroecosystems. Abundance and diversity were much higher in the rainy than in the dry season. Rhodacarids and ascids have not been used commercially for pest control, but investigations conducted so far suggest their potential as biological control agents. The confirmation of this possibility and the development of techniques that would allow their maintenance in agricultural areas require subsequent research efforts.

Beschreibung: The most massive and fast-eroding thaw slump of the Northern Hemisphere located in the Yana Uplands of Northern Yakutia was investigated to assess in detail the cryogenic inventory and carbon pools of two distinctive Ice Complex stratigraphic units and the uppermost cover deposits. Differentiating into modern and Holocene near-surface layers (active layer and shielding layer), highest total carbon contents were found in the active layer (18.72 kg m−2), while the shielding layer yielded a much lower carbon content of 1.81 kg m−2. The late Pleistocene upper Ice Complex contained 10.34 kg m−2 total carbon, and the mid-Pleistocene lower Ice Complex 17.66 kg m−2. The proportion of organic carbon from total carbon content is well above 70% in all studied units with 94% in the active layer, 73% in the shielding layer, 83% in the upper Ice Complex and 79% in the lower Ice Complex. Inorganic carbon is low in the overall structure of the deposits.

Beschreibung: Organic amendments can reportedly sustain and increase lowland rice productivity in smallholder systems. Few studies have assessed locally-available substrates in hydrologically variable floodplain environments. We investigated the effects of green and farmyard manures on rice yields, and total soil C and N in the Kilombero floodplain, Tanzania. At both the fringe and the middle positions, five treatments were applied in 2016 and 2017, comprising (1) non-amended control, (2) farmyard manure, (3) pre-rice legumes, (4) post-rice legumes and (5) a combination of green and farmyard manures. Residual treatment effects were assessed in 2018 when rice plots were uniformly non-amended. Depending on the year and the position, organic amendments increased rice grain yields by 0.7–3.1 Mg ha–1 above the non-amended control. Sole green and farmyard manure applications had similar effects on grain yield, while a combination of green and farmyard manure led to a significant increase in grain yield above both the control and sole applications of organic amendments in both years. The contribution from biological N2 fixation by legumes ranged from 4 to 61 kg N ha–1. Despite partial N balances being mostly negative, we observed positive residual effects on the yield of the non-amended rice in the third year. Such effects reached up to 4 Mg ha−1 and were largest with post-rice legumes, sole or combined with farmyard manure. Irrespective of the position in the floodplain, manures significantly increased soil C and N contents after two years, hence enhancing soil fertility and resulting in increased rice grain yields. Comparable benefits may be obtained along the hydrological gradients of other large river floodplains of the region and beyond.

Beschreibung: The aim of this study was to analyse the genetic and phenotypic differentiation of bacteria isolated from root nodules of Trifolium rubens, a rare clover species. The symbiotic compatibility of selected isolates was investigated using two agronomically important plants, red clover (Trifolium pratense L.) and pea (Pisum sativum L.). A high genetic diversity of analysed strains was shown using the Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) method. Most of the strains showed a high similarity to R. leguminosarum in the 16S rRNA sequence. Two strains were identified as Agrobacterium spp. There was a strong positive correlation between fresh clover weight and the number of root nodules and a statistically significant increase in the number of the root nodules in selected strains compared to the negative control. Phenotypic tests and BIOLOG analysis showed the ability of the analysed strains to grow in different experimental conditions (pH = 6–10, 0.5–3% NaCl, different carbon and nitrogen sources), which makes them suitable for application as an agronomically important factor.

Beschreibung: Ecuador Andean purple corn (Zea mays L.) was subjected to a germination process at 15–40 °C for 24–168 incubation hours. Purple corn protein concentrates (PCPCs) were obtained by alkaline extraction at pH 8.0 and pH 10.0, followed by an isoelectric precipitation process at pH 4.0, pH 5.0 and pH 6.0. Proteins and phenolic content of PCPCs was calculated. PCPC antioxidant properties were determined by the ferric-reducing antioxidant power (FRAP) in vitro method and by the 2,2-azinobis, 3-ethyl-benzothiazoline-6-sulfonic acid, (ABTS) in vitro method. Andean purple corn seeds were able to germinate under the germination conditions tested in this study. The higher percentage of germination was of 63.33% at 168 h/25 °C. The PCPCs protein profile was characterized for the presence of six bands with molecular weights of 14.50 kDa, 20.12 kDa, 25.18 kDa, 41.85 kDa, 59.59 kDa, and 65.87 kDa. Germinated PCPC presented a high TPC content with ranges of 605.71–1820.00 mg gallic acid equivalents (GAE)/g PCPC dry weight (DW), germinated PCPC/72 h/25 °C presented a higher value of 1820.00 mg GAE/g PCPC, DW. All germinated PCPCs samples assayed presented strong antioxidant activity when measured by the ABTS and FRAP methods. Germinated PCPC/144 h/35 °C presented high antioxidant activity by ABTS with 804.35 µmol Trolox equivalents (TE)/g PCPC DW and germinated PCPC/144 h/30 °C presented a high value by the FRAP method, 987.83 µmol TE/g PCPC DW.

Beschreibung: Weedy rice is one of the most common weeds in rice cultivation in many rice areas throughout the world and it is able to cause significant yield reductions. Weedy rice is characterized by a high biological diversity that permits different populations to be identified on the basis of their morphological and physiological traits. This variability contributes to its success in different environments and allows different abiotic stresses, which are intensified by climate change, to be faced. Taller plants, enhanced tillering, seed shattering and the presence of red pericarp, variable hull coloration and awn morphology, linked to a deeper seed dormancy, are some of the traits that help weedy rice to spread in changing environments. The higher phenotypic plasticity and genetic variability of weedy rice make it more able to cope with temperature variations, intermittent water availability, soil salinity, drought conditions and increased CO2 concentrations than cultivated rice. As these abiotic stresses will become more frequent in the future, weedy rice competitiveness may be higher, with a spread of infestations. Thus, the control of weedy rice should be based on an integration of different preventive and agronomic techniques, a sensible use of herbicides and the use of suitable rice varieties.

Beschreibung: Testing Fusarium resistance to ear rots in maize requires a well-supported methodology and tests for toxin responses. In this study, commercial hybrids were tested for resistance to Fusarium graminearum, Fusarium culmorum, and Fusarium verticillioides (kernel and silk channel), as well as their toxin response. One third of the hybrids tested showed a similar resistance or susceptibility to the three pathogens and their toxin response, but there is no proof for their genetic background being the same or different. The performance of the remaining hybrids was highly variable and supports the idea of different genetic regulation. The mean ear rot severity of the kernel resistance was doubled compared with the silk channel resistance data. The ear rot and toxin tests displayed significant positive correlations, verifying the decisive role of resistance in toxin regulation. Several hybrids, termed toxigenic hybrids, showed significant extra toxin production, indicating an additional food safety risk. The toothpick method gave more reliable results and a better differentiation of genotypes. The resistance to different Fusarium spp. in a specific growing region should be analyzed separately in independent resistance tests. Through this, the food safety risks could be better identified. Susceptible hybrids should not be used for commercial production.

Beschreibung: The study’s objective was to evaluate the influence of thinning on the quality and regularity of yield of ‘Regina’ cherries grown on a dwarf Gisela 5 rootstock. The experiments were conducted in the years 2009–2012 in Western Poland. Trees were thinned using a chemical agent, ammonium thiosulphate (ATS), at doses of 20 g ATS L−1, 30 g ATS L−1 and 40 g ATS L−1, and by hand, and the results of chemical and hand thinning were compared with those obtained for unthinned trees. The course of weather conditions in winter and during flowering had a significant impact on yield. Chemical thinning is known to be an effective method to regulate fruiting of pome trees, but it can also be successful if applied to cherry trees, as reflected by the alternate bearing index, which was the lowest after applying ATS at a dose of 40 g. ATS treatment improved fruit quality in full crop years. Thinning resulted in, among other things, larger and darker fruit and higher content of total soluble solids and titratable acidity. Treatment with 40 g ATS L−1 significantly stimulated the vegetative growth of trees.

Beschreibung: Emerald ash borer is an invasive pest in North American forests. Ecological impacts of ash mortality from emerald ash borer are wide-ranging, including shifts in insect communities and wildlife behavior. Additionally, loss of ash from forests may have important implications regarding plant succession. Surveys of overstory, midstory, and understory trees within forests in northeastern Indiana, Lower Peninsula of Michigan, and northwestern Ohio were conducted to quantify the change in forest composition over a 10 year period. Interpolation of ash dominance illustrated inversion of live and dead ash values between 2007 and 2017. Even though more than 83% of overstory live ash basal area was lost across the study area, green ash was the most abundant midstory and understory species representing regeneration. Additionally, loss of ash from many of the sites resulted in compositional changes that were greater than merely the subtraction of ash. Due to the relatively large number of forest types with which ash species are associated, loss of ash will have broad ecological consequences, including on community composition.

Beschreibung: Since 2006, an increasing number of French vineyards have chosen to convert to organic farming. One major change in vineyard practices includes replacing chemical pesticides with copper and sulfur-based products in line with Council Regulation (EC) No. 834/2007. This change can make overall management and pest and disease control more difficult and potentially lead to yield losses. From 2013 to 2016, a network of 48 vineyard plots, in southern France, under conventional management and in conversion to organic farming were monitored throughout the three-year conversion phase to investigate the grapevine phytosanitary management of four major pests and diseases and variations in control efficiency. The severity of downy and powdery mildew, grape berry moths, and Botrytis bunch rot were assessed and linked to the protection strategy. The findings showed that pests and diseases were controlled in the third year of conversion at similar efficiency levels as in conventional farming. However, the first two years of conversion were a transitional and less successful period during which higher incidences of cryptogamic diseases were observed. This demonstrates a need for winegrowers to receive more in-depth technical advice and support, especially on pest and disease control, during this critical transition period.

Beschreibung: Increasing demands on animal welfare and the higher temperatures in summer due to climate change make it necessary to adapt conventional pig husbandry systems. A cooled, solid lying area has the potential to increase lying comfort and reduce the heat stress, which improves animal welfare. In the present study, the effect of a cooled, solid lying area on lying and elimination behavior was investigated. In two fattening compartments, eight pens with 28 pigs each were rebuilt. Two pen designs, different in feeder place and type, were tested. The floor was cooled from 24.5 to 20 °C by cool water in half of the pens. A total of 672 fattening pigs were tested over three fattening periods. The lying behavior was recorded by video analysis three times per week and three times per day. In the pens with a cooled lying area, 14% (SED 2.9; p = 0.002), respectively 12% (SED 0.9; p = 0.0382) more pigs were lying on the solid lying area. Additionally, the fouling of the animals was reduced by the cooling in one pen design; the results were derived from weekly scores (0.42 vs. 0.67; SED 0.058 p = 0.0006). The fouling of the pen was not affected by the floor cooling, however, the fouling in all pens was very low.

Beschreibung: The sugarcane (Saccharum spp. L.) mechanical harvesting system leaves a large amount of straw mulch on the soil surface. The straw mulch may affect soil conditions, root regrowth, and sugarcane yield. Thus, this study assessed the response of sugarcane root system growth and stalk yield to different rates of straw removal. An experiment was conducted in a Rhodic Kandiudox with sand clay loam texture to test the impact of four rates of straw removal: no removal (18.9 Mg ha−1 of dry mass); moderate removal (8.7 Mg ha−1); high removal (4.2 Mg ha−1) and total removal on sugarcane root system and stalk yield. Higher concentrations of roots (60%) were found in the first 40 cm of soil. Moderate straw removal resulted in higher root mass (3.6 Mg ha−1) and stalk production (23 Mg ha−1 of dry mass). However, no straw removal reduced root mass by 20% (105 Mg ha−1). Through regression analysis, it was estimated that retaining between 8.5 and 13 Mg ha−1 of straw resulted in the highest root mass and stalk yield. Managing straw removal to retain a moderate amount enables producers to sustain suitable soil conditions for sugarcane root growth and stalk production while providing straw for industrial use.

Beschreibung: Proximal sensing techniques can potentially survey soil and crop variables responsible for variations in crop yield. The full potential of these precision agriculture technologies may be exploited in combination with innovative methods of data processing such as machine learning (ML) algorithms for the extraction of useful information responsible for controlling crop yield. Four ML algorithms, namely linear regression (LR), elastic net (EN), k-nearest neighbor (k-NN), and support vector regression (SVR), were used to predict potato (Solanum tuberosum) tuber yield from data of soil and crop properties collected through proximal sensing. Six fields in Atlantic Canada including three fields in Prince Edward Island (PE) and three fields in New Brunswick (NB) were sampled, over two (2017 and 2018) growing seasons, for soil electrical conductivity, soil moisture content, soil slope, normalized-difference vegetative index (NDVI), and soil chemistry. Data were collected from 39–40 30 × 30 m2 locations in each field, four times throughout the growing season, and yield samples were collected manually at the end of the growing season. Four datasets, namely PE-2017, PE-2018, NB-2017, and NB-2018, were then formed by combing data points from three fields to represent the province data for the respective years. Modeling techniques were employed to generate yield predictions assessed with different statistical parameters. The SVR models outperformed all other models for NB-2017, NB-2018, PE-2017, and PE-2018 dataset with RMSE of 5.97, 4.62, 6.60, and 6.17 t/ha, respectively. The performance of k-NN remained poor in three out of four datasets, namely NB-2017, NB-2018, and PE-2017 with RMSE of 6.93, 5.23, and 6.91 t/ha, respectively. The study also showed that large datasets are required to generate useful results using either model. This information is needed for creating site-specific management zones for potatoes, which form a significant component for food security initiatives across the globe.