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  • 2015-2019  (742,503)
  • 1955-1959  (5)
  • 2015  (742,503)
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Years
Year
  • 1
    Monograph available for loan
    Monograph available for loan
    Garmisch-Partenkirchen : Institut für atmosphärische Umweltforschung der Fraunhofer- Gesellschaft
    Call number: MOP 44829 / Mitte
    Type of Medium: Monograph available for loan
    Pages: 25 S. , graph. Darst.
    Language: English
    Location: MOP - must be ordered
    Branch Library: GFZ Library
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  • 2
    Call number: IASS 15.89494
    Type of Medium: Monograph available for loan
    Pages: Losebl.-Ausg.
    Edition: Stand: Oktober 2010
    ISBN: 9783768501828
    Language: German
    Branch Library: RIFS Library
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  • 3
    Call number: MOP 19538/1d-6d
    Type of Medium: Monograph available for loan
    Pages: 111 S.
    ISSN: 0486-2287
    Language: Russian
    Note: In kyrill. Schr.
    Location: MOP - must be ordered
    Branch Library: GFZ Library
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  • 4
    Call number: Z 06.0500
    Type of Medium: Journal available for loan
    Pages: 30 cm
    ISSN: 1824-7741
    Former Title: Vorgänger Geologisch-paläontologische Mitteilungen, Innsbruck
    Language: German , English
    Note: Ersch. unregelmäßig , Beiträge teilweise in Englisch
    Branch Library: GFZ Library
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  • 5
    Monograph available for loan
    Monograph available for loan
    [Edgecumbe, N.Z.] : A. Muller
    Call number: M 15.89146
    Description / Table of Contents: An account of the results of the 2 March 1987 earthquake in the eastern Bay of Plenty and the aftermath's effects on the people and places on the Rangitaiki Plains
    Type of Medium: Monograph available for loan
    Pages: 223 S., , Ill.
    Language: English
    Branch Library: GFZ Library
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  • 6
  • 7
    Call number: S 90.0066(162,1)
    In: Geologisches Jahrbuch / A
    Type of Medium: Series available for loan
    Pages: 261 Seiten , Ill., 1 DVD-ROM (12 cm) und 1 Tafel-Beil. ([2] S.)
    ISBN: 9783510968534
    Series Statement: Geologisches Jahrbuch 162
    Classification:
    Engineering Geophysics
    Language: German
    Location: Lower compact magazine
    Branch Library: GFZ Library
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  • 8
    Monograph available for loan
    Monograph available for loan
    Stuttgart : Schweizerbart Science Publishers ; Volume 1, number 1 (1978)-
    Call number: M 18.91571
    Type of Medium: Monograph available for loan
    Pages: 134 Seiten
    ISSN: 2363-7196
    Series Statement: Global tectonics and metallogeny : special issue Vol. 10/2-4
    Classification:
    Tectonics
    Parallel Title: Erscheint auch als Global tectonics and metallogeny
    Language: English
    Location: Upper compact magazine
    Branch Library: GFZ Library
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  • 9
    Call number: AWI G6-19-92375
    In: Berichte / Christian-Albrechts-Universität zu Kiel, Institut für Geowissenschaften, Nr. 9
    Type of Medium: Monograph available for loan
    Pages: 278 Seiten , Illustrationen
    ISSN: 0175-9302
    Series Statement: Berichte / Christian-Albrechts-Universität zu Kiel, Institut für Geowissenschaften 9
    Language: German
    Note: Zugleich: Dissertation, Christian-Albrechts-Universität zu Kiel, 1999 , INHALTSVERZEICHNIS 1. Einleitung 1.1 Kenntnisstand und offene Fragen 1.2 Fragestellung und Ziele dieser Arbeit 2. Umweltbedingungen in den Arbeitsgebieten 2.1 Hydrographie, Eisverhältnisse und NAO 2.2 Zur Variation von Wassertiefe und Breite der Dänemarkstraße und zur Vereisung Islands während des letzten Glazials 3. Methoden 3.1 Auswahl der Kernstationen 3.2 Probennahme und Analysen (Übersicht) 3.3 Zur Rekonstruktion von Paläobedingungen im Oberflächenwasser Zur Aussage stabiler Isotopenverhältnisse in planktischen Foraminiferen Zur Messung stabiler Isotopenverhältnisse Zur Massenspektrometrie Zur Rekonstruktion von Oberflächentemperaturen Alkane und Alkohole als Maß für Staubeintrag Eistranspmtiertes Material und vulkanische Aschen 3.4 Zur Rekonstruktion von Paläobedingungen im Zwischen-/ Tiefenwasser Häufigkeit von Cibicides- und anderen benthischen Arten (inkl. Taxonomie) Stabile Isotopenverhältnisse in benthischen Foraminiferen 3.5 AMS 14C-Datierungen Probenreinigung 3. 6 Hauptelementanalysen von vulkanischen Asche-Leithorizonten 3. 7 Geomagnetische Meßgrößen und magnetische Suszeptibiltät 3.8 Techniken zur Spektralanalyse 4. Methodische Ergebnisse 4.1 Zum Einfluß der Probenreinigung auf δ18O-/ δ13C-Werte 4.2 Probleme bei der langfristigen Reproduzierbarkeit von δ18O-Zeitreihen 4.3 Einfluß der Korngröße und Artendefinition planktischer Foraminiferen auf SST-Rekonstruktionen in hohen Breiten 4.4 Vergleich der stabilen Isotopenwerte von Cibicides lobatulus und Cibicidoides wuellerstorfi 5. Stratigraphische Grundlagen und Tiefenprofile der Klimasignale 5.1 Stratigraphische Korrelation zwischen parallel-gekernten GKG- und SL-/KL-Profilen 5.2 Flanktische δ18O-/ δ13C-Kurven, 14C-Alter und biostratigraphische Fixpunkte Westliches Islandbecken Kern PS2644 Kern PS2646 Kern PS2647 Kern 23351 Vøring-Plateau Kern 23071 Kern 23074 5.3 Benthische δ18O-/ δ13C-Werte in Kern PS2644 5.4 Siliziklastische Sedimentkomponenten: Eistransportiertes Material Westliches Islandbecken Kern PS2644 Kern PS2646 Kern PS2647 Vøring-Plateau Kern 23071 Kern 23074 5.5 Vulkanische Glasscherben in Kern PS2644: Wind- und Eiseintrag 5.6 Geochemie und Alter einzelner Tephralagen als Leithorizonte Westliches Islandbecken Kern PS2644 Kern PS2646 Kern PS2647 Vøring-Plateau Kern 23071 Kern 23074 5.7 Magnetische Suszeptibilität in den Kernen PS2644, PS2646 und PS2647 Kern PS2644 Kern PS2646 und PS2647 5.8 Geomagnetische Feldintensität und Richtungsänderungen in Kern PS2644 5.9 Variation von Planktonfauna und -flora Westliches Islandbecken: Kern PS2644 Kern PS2646 und PS2647 Vøring-Plateau: Kern 23071 und 23074 5.10 Benthische Foraminiferen in Kern PS2644 6. Entwicklung von Temperatur und Salzgehalt nördlich der Dänemark-Straße 6.1 Variation der Oberflächentemperatur nach Planktonforaminiferen 6.2 Variation der Oberflächentemperatur nach Uk37 6.3 Variation der Oberflächensalinität 7. Die Feinstratigraphie von Kern PS2644 als Basis für eine Eichung der 14C-Altersskala 22 - 55 ka 7.1 Korrelation zwischen den Klimasignalen in Kern PS2644 und der GISP2-Klimakurve zum Kalibrieren der 14C-Alter und Erstellen eines Altersmodells Tephrachronologische Marker Korrelationsparameter und -regeln Sonderfälle/ Probleme bei der Korrelation 7.2 Alters-stratigraphische Korrelation der Klimakurven von Kern 23071 und 23074 7.3 Variation der Altersanomalien zwischen 20 und 55 14C-ka 7.4 Variabilität des planktischen 14C-Reservoiralters in Schmelzwasserbeeinflußten Seegebieten Variation der planktischen 14C-Alter unmittelbar an der Basis von Heinrich-Ereignis 4 Unterschiede zwischen planktischen und benthischen 14C-Altern in der westlichen Islandsee. Zur Erklärung der inversen Altersdifferenzen 7.5 Differenz zwischen 14C- und Kalenderalter: Zeitliche Variation unter Einfluß des Erdmagnetfeldes - Modell und Befund 7.6 Sedimentationsraten der Kerne 23071, 23074 und PS2644 nach dem GISP2-Altersmodell Vøring-Plateau: Kerne 23071 und 23074 Südwest-Islandsee: Kern PS2644 8. Klimaoszillationen im Europäischen Nordmeer in der Zeit und Frequenzdomäne 8.1 "Der Einzelzyklus" in den Klimakurven von Kern PS2644 8.2 Zur Veränderlichkeit der Warm- und Kaltextreme sowie Zyklenlänge Besonderheiten in der Zyklenlänge Variation der Kalt-(Stadiale) Variation der Interstadiale 8.3 Periodizitäten der Klimasignale im Frequenzband der D.-Oe.-Zyklen. Der D.-Oe.-Zyklus von 1470 J., seine Multiplen und harmonischen Schwingungen Weitere Frequenzen: 1000-1150 Jahre- und 490- 510 Jahre-Zyklizitäten Höhere Frequenzen im Bereich von Jahrhunderten und Dekaden 8.4 Phasenbeziehungen und (örtliche) Steuemngsmechanismen der Dansgaard-Oeschger-Zyklen 9. Schlußfolgerungen Danksagung Literaturverzeichnis Anhang
    Location: AWI Reading room
    Branch Library: AWI Library
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  • 10
    Call number: AWI A3-20-93434-2
    In: Meteorologische Abhandlungen / Institut für Meteorologie und Geophysik der Freien Universität Berlin, Band XXXII, Heft 2
    Type of Medium: Series available for loan
    Pages: 218 Seiten , Illustrationen
    Series Statement: Meteorologische Abhandlungen / Institut für Meteorologie und Geophysik der Freien Universität Berlin 32,2
    Language: German
    Note: Zugleich: Dissertation, Freie Unversität Berlin, [ca. 1963] , INHALTSVERZEICHNIS PROBLEMSTELLUNG UND ZIELSETZUNG 1. BEMERKUNGEN ZUM BEOBACHTUNGSGELÄNDE UND ZUM BEOBACHTUNGSMATERIAL 1.1 Das Beobachtungsgelände 1.2 Das Beobachtungsmaterial 2. HOMOGENITÄTSBETRACHTUNGEN 2.1 Temperatur 2.2 Niederschlag 2.3 Wind 2.4 Sonnenschein und Bewölkung 3. TEMPERATURVERHÄLTNISSE 3.1 Monats- und Jahreswerte 3.2 Tageswerte 3.3 Pentadenwerte 3.4 Häufigkeitsbetrachtungen 3.5 Interdiurne Veränderlichkeit 3.6 Der tägliche Gang 3.7 Vorkommen bestimmter Schwellenwerte 3.71 Frost- und Eistage 3.72 Sommer- und Tropentage 4. DER WASSERGEHALT DER LUFT 4.1 Monats- und Jahreswerte 4.2 Tageswerte 4.3 Häufigkeitsbetrachtungen 4.4 Interdiurne Veränderlichkeit 4.5 Der tägliche Gang 5. BEWÖLKUNGSVERHÄLTNISSE 5.1 Monats- und Jahreswerte 5.2 Tageswerte 5.3 Häufigkeitsbetrachtungen 5.4 Der tägliche Gang 5.5 Heitere und trübe Tage 5.6 Nebel 6. SONNENSCHEIN 6.1 Monats- und Jahreswerte 6.2 Tageswerte 6.3 Der tägliche Gang 7. NIEDERSCHLAGSVERHÄLTNISSE 7.1 Monats- und Jahreswerte 7.2 Niederschlagsbereitschaft 7.3 Tageswerte 7.4 Der tägliche Gang 7.5 Häufigkeitsbetrachtungen 7.6 Niederschlags- und Trockenperioden 7.7 Niederschlag und Wind· 7.8 Schneeverhältnisse 7.81 Schneefall und Schneedecke 7.82 Schneehöhe 7.9 Gewitter 8. WINDVERHÄLTNISSE 8.1 Windrichtung 8.2 Windgeschwindigkeit 8.21 Der jährliche Gang 8.22 Häufigkeitsbetrachtungen 8.23 Sturmtage und Windstillen 8.24 Der tägliche Gang 9.ZUSAMMENFASSUNG VERZEICHNIS DER TEXTTABELLEN VERZEICHNIS DER ABBILDUNGEN LITERATURVERZEICHNIS TABELLENANHANG
    Location: AWI Reading room
    Branch Library: AWI Library
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  • 11
    Monograph available for loan
    Monograph available for loan
    London : Penguin Books
    Type of Medium: Monograph available for loan
    ISBN: 9780141985206
    Language: English
    Branch Library: RIFS Library
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  • 12
    Call number: (DE-599)GBV03709842X
    Type of Medium: Monograph available for loan
    Language: German
    Location: MOP - must be ordered
    Branch Library: GFZ Library
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  • 13
    Monograph non-lending collection
    Monograph non-lending collection
    Leiden : Nijhoff ; 1.2009 -
    Call number: IASS 17.92082
    Type of Medium: Monograph non-lending collection
    ISSN: 1876-8814
    Language: English
    Branch Library: RIFS Library
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  • 14
    Monograph available for loan
    Monograph available for loan
    Leningrad : Gidrometeorolog. Izd.
    Call number: MOP 33767
    Type of Medium: Monograph available for loan
    Pages: 663 S.
    Language: Russian
    Note: In kyrill. Schr., russ.
    Location: MOP - must be ordered
    Branch Library: GFZ Library
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  • 15
    Journal available for loan
    Journal available for loan
    München : Altop Verlag ; 2007 -
    Call number: Z 19.92410
    Type of Medium: Journal available for loan
    Pages: 30 cm
    ISSN: 1865-4266
    Former Title: Vorg. Nachhaltiges Wirtschaften in Deutschland
    Language: German
    Note: Ungezählte Beil. ab 2010: Special , Ersch. jährl. 4x
    Branch Library: RIFS Library
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  • 16
    Journal available for loan
    Journal available for loan
    Tübingen : Mohr Siebeck ; 1.1884 - 48.1931; N.F. 1.1932/33 - 10.1943/44(1945),3; 11.1948/49(1949) -
    Call number: ZS 22.95039
    Type of Medium: Journal available for loan
    Pages: Online-Ressource
    ISSN: 1614-0974 , 0015-2218 , 0015-2218
    Language: German , English
    Note: N.F. entfällt ab 57.2000. - Volltext auch als Teil einer Datenbank verfügbar , Ersch. ab 2000 in engl. Sprache mit dt. Hauptsacht.
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  • 17
    Monograph available for loan
    Monograph available for loan
    Madrid : Secc
    Call number: PIK N 456-17-90913
    Type of Medium: Monograph available for loan
    Pages: 536 Seiten
    Series Statement: Ministerio de Transportes Turismo Y Comunicaciones : Publicación Serie A 114
    Parallel Title: 1,1=6; 2,1=13 von Publicaciones / D / Ministerio del Aire, Subsecretaria de Aviación Civil, Servicio Meteorológico Nacional
    Language: Spanish
    Location: A 18 - must be ordered
    Branch Library: PIK Library
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  • 18
    Call number: 3/S 07.0034(2016)
    In: Annual report
    Type of Medium: Monograph available for loan
    Pages: 51 Seiten
    ISSN: 1865-6439 , 1865-6447
    Parallel Title: Erscheint auch als Annual report ... / Helmholtz Association of German Research Centres
    Language: English
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  • 19
    Call number: AWI A3-20-93434
    In: Meteorologische Abhandlungen / Institut für Meteorologie und Geophysik der Freien Universität Berlin, Band XXXII, Heft 1
    Type of Medium: Series available for loan
    Pages: 121 Seiten , Illustrationen
    Series Statement: Meteorologische Abhandlungen / Institut für Meteorologie und Geophysik der Freien Universität Berlin 32,1
    Language: German
    Note: Zugleich: Dissertation, Freie Unversität Berlin, [ca. 1963] , INHALTSVERZEICHNIS PROBLEMSTELLUNG UND ZIELSETZUNG 1. BEMERKUNGEN ZUM BEOBACHTUNGSGELÄNDE UND ZUM BEOBACHTUNGSMATERIAL 1.1 Das Beobachtungsgelände 1.2 Das Beobachtungsmaterial 2. HOMOGENITÄTSBETRACHTUNGEN 2.1 Temperatur 2.2 Niederschlag 2.3 Wind 2.4 Sonnenschein und Bewölkung 3. TEMPERATURVERHÄLTNISSE 3.1 Monats- und Jahreswerte 3.2 Tageswerte 3.3 Pentadenwerte 3.4 Häufigkeitsbetrachtungen 3.5 Interdiurne Veränderlichkeit 3.6 Der tägliche Gang 3.7 Vorkommen bestimmter Schwellenwerte 3.71 Frost- und Eistage 3.72 Sommer- und Tropentage 4. DER WASSERGEHALT DER LUFT 4.1 Monats- und Jahreswerte 4.2 Tageswerte 4.3 Häufigkeitsbetrachtungen 4.4 Interdiurne Veränderlichkeit 4.5 Der tägliche Gang 5. BEWÖLKUNGSVERHÄLTNISSE 5.1 Monats- und Jahreswerte 5.2 Tageswerte 5.3 Häufigkeitsbetrachtungen 5.4 Der tägliche Gang 5.5 Heitere und trübe Tage 5.6 Nebel 6. SONNENSCHEIN 6.1 Monats- und Jahreswerte 6.2 Tageswerte 6.3 Der tägliche Gang 7. NIEDERSCHLAGSVERHÄLTNISSE 7.1 Monats- und Jahreswerte 7.2 Niederschlagsbereitschaft 7.3 Tageswerte 7.4 Der tägliche Gang 7.5 Häufigkeitsbetrachtungen 7.6 Niederschlags- und Trockenperioden 7.7 Niederschlag und Wind· 7.8 Schneeverhältnisse 7.81 Schneefall und Schneedecke 7.82 Schneehöhe 7.9 Gewitter 8. WINDVERHÄLTNISSE 8.1 Windrichtung 8.2 Windgeschwindigkeit 8.21 Der jährliche Gang 8.22 Häufigkeitsbetrachtungen 8.23 Sturmtage und Windstillen 8.24 Der tägliche Gang 9.ZUSAMMENFASSUNG VERZEICHNIS DER TEXTTABELLEN VERZEICHNIS DER ABBILDUNGEN LITERATURVERZEICHNIS TABELLENANHANG
    Location: AWI Reading room
    Branch Library: AWI Library
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  • 20
    Call number: IASS 22.95033
    Type of Medium: Monograph available for loan
    Pages: 378 S , 225 mm x 135 mm
    ISBN: 3899421876 , 978-3-89942-187-3
    Series Statement: Edition panta rei
    Language: German
    Note: Zugl.: Marburg (Lahn), Univ., Habil.-Schr., 2004 u.d.T.: Gutmann, Mathias: Die Medialität des Erfahrens
    Branch Library: RIFS Library
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  • 21
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 294 (1992), S. 466-478 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 22
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 317 (1993), S. 474-484 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 23
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    Wien : [Verlag nicht ermittelbar] ; 22.1910/25(1925),3; 23.1914/31(1929/31),2-3; 24.1927,1-2; 25.1939,1; 26.1948,1; 27.1971-Band 76 (2022)
    Call number: S 91.1179
    ISSN: 0375-5797 , 0378-0864
    Parallel Title: 35=2 von European Conodont Symposium (ZDB) Guidebook, abstracts / European Conodont Symposium
    Parallel Title: 41=2 von Workshop on Agglutinated Foraminifera (ZDB) Proceedings / Workshop on Agglutinated Foraminifera. Geologische Bundesanstalt
    Parallel Title: 39=3 von International Nannoplankton Association Proceedings of the ... International Nannoplankton Association conference
    Parallel Title: 60=11 von Deutsche Gesellschaft für Geowissenschaften. Fachsektion GeoTop Internationale Jahrestagung der Fachsektion GeoTop der Deutschen Gesellschaft für Geowissenschaften
    Former Title: Vorg. Geologische Reichsanstalt Abhandlungen der Kaiserlich-Königlichen Geologischen Reichsanstalt, Wien
    Subsequent Title: Fortgesetzt durch Abhandlungen
    Language: German
    Branch Library: GFZ Library
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  • 24
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. '1pctCo2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.1 1pctCo2 (6.1 1 percent per year CO2) - Version 1: Idealized 1% per year increase in atmospheric CO2 to quadrupling. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 25
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. historicalMisc is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc . Used forcings in individual ensemble runs (see attached addinfo for more information): r[1-5]i1p1 - Ant: anthropogenic forcing (AA anthropogenic aerosols - a mixture of aerosols, not explicitly defined here; Oz - tropospheric and stratospheric ozone; GHG - well-mixed greenhouse gases, a mixture, not explicitly defined here); r[1-5]i1p2 - NoOz: all forcings except tropospheric and stratospheric ozone concentrations fixed; r[1-5]i1p3 - NoAA: all forcings except anthropogenic aerosols (AA) and the indirect effect of AA on snow albedo; r[1-5]i1p4 - AA: anthropogenic aerosols (AA) including the indirect effect of AA on snow albedo; r[1-5]i1p5 - AntNoAA: the same as NoAA (AA is fixed at preindustrial levels), except that AA emissions are allowed to vary in the Asian region [10S to 45N x 65E to 150E]; r[1-5]i1p6 - VI: volcanic forcing only.
    Type: dataset_group
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  • 26
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClim' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.2a sstClim (6.2a Control SST Climatology) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
    Format: NetCDF
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  • 27
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClimAerosol' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.4a sstClimAerosol (6.4a all aerosol forcing) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice (as in expt. 6.2a) but with aerosols consistent with conditions in year 2000 of the historical run (expt. 3.2) Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
    Format: NetCDF
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  • 28
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'abrupt4xco2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.3 abrupt4xco2 (6.3 Abrupt 4XCO2) - Version 1: Impose an instantaneous quadrupling of CO2, then hold fixed. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 29
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClim' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.2a sstClim (6.2a Control SST Climatology) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 30
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. '1pctCo2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.1 1pctCo2 (6.1 1 percent per year CO2) - Version 1: Idealized 1% per year increase in atmospheric CO2 to quadrupling. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 31
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. midHolocene is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. midHolocene (3.4 mid-Holocene) - Version 2: Consistent with PMIP (Paleo Model Intercomparison Project) specifications. Impose Mid-Holocene (6 kyrs ago) conditions including Orbital parameters and Atmospheric concentrations of well-mixed greenhouse gasses. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 32
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'amip' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.3 amip (3.3 AMIP) - Version 1: AMIP (1979 - at least 2008). Impose SSTs and sea ice from observations but with other conditions as in experiment 3.2 historical. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 33
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'historicalNat' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 7.1 historicalNat (7.1 natural-only historical simulation) - Version 1: Historical simulation but with natural forcing only. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 34
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'abrupt4xco2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.3 abrupt4xco2 (6.3 Abrupt 4XCO2) - Version 1: Impose an instantaneous quadrupling of CO2, then hold fixed. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 35
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'rcp45' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.1 rcp45 (4.1 RCP4.5) - Version 1: Future projection (2006-2100) forced by RCP4.5. RCP4.5 is a representative concentration pathway which approximately results in a radiative forcing of 4.5 W m-2 at year 2100, relative to pre-industrial conditions. RCPs are time-dependent, consistent projections of emissions and concentrations of radiatively active gases and particles. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 36
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. historicalExt is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 7.4 historicalExt (7.4 Historical Extension) - Version 1: Extend the CMIP5 historical runs to the near-present (as we have for AMIP), rather than ending them in 2005. Simulations extended to at least the end of 2012 using some estimate of recent and future forcing. Groups are free to use whatever concentrations, solar forcing, SO2 emissions etc. they want to use in extending these runs. It is recommended that if an ensemble of "all-forcings" historical simulations have been run, then *each* member of the ensemble should be carried to the end of 2012. If some other forcing is used or if the run is an RCP run that is truncated after a few years, then the run should be considered a "historical extension" experiment with its output placed in a directory named historicalExt. For these historicalExt experiments, the ensemble member (designated by the "rip" value appearing in the filename and recorded as netCDF global attributes) will be identical to the historical run it extends. List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 37
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'amip' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.3 amip (3.3 AMIP) - Version 1: AMIP (1979 - at least 2008). Impose SSTs and sea ice from observations but with other conditions as in experiment 3.2 historical. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 38
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. piControl is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.1 piControl (3.1 Pre-Industrial Control) - Version 1: Pre-Industrial coupled atmosphere/ocean control run. Imposes non-evolving pre-industrial conditions. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 39
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. historicalExt is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 7.4 historicalExt (7.4 Historical Extension) - Version 1: Extend the CMIP5 historical runs to the near-present (as we have for AMIP), rather than ending them in 2005. Simulations extended to at least the end of 2012 using some estimate of recent and future forcing. Groups are free to use whatever concentrations, solar forcing, SO2 emissions etc. they want to use in extending these runs. It is recommended that if an ensemble of "all-forcings" historical simulations have been run, then *each* member of the ensemble should be carried to the end of 2012. If some other forcing is used or if the run is an RCP run that is truncated after a few years, then the run should be considered a "historical extension" experiment with its output placed in a directory named historicalExt. For these historicalExt experiments, the ensemble member (designated by the "rip" value appearing in the filename and recorded as netCDF global attributes) will be identical to the historical run it extends. List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 40
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'historical' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.2 historical (3.2 Historical) - Version 1: Simulation of recent past (1850 to 2005). Impose changing conditions (consistent with observations). Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 41
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'historical' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.2 historical (3.2 Historical) - Version 1: Simulation of recent past (1850 to 2005). Impose changing conditions (consistent with observations). Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 42
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClim4xco2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.2b sstClim4xco2 (6.2b SST Climatology With 4XCO2 Forcing) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice (as in 6.2a) but with quadrupled 4XCO2 imposed. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 43
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. piControl is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.1 piControl (3.1 Pre-Industrial Control) - Version 1: Pre-Industrial coupled atmosphere/ocean control run. Imposes non-evolving pre-industrial conditions. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 44
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. '1pctCo2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.1 1pctCo2 (6.1 1 percent per year CO2) - Version 1: Idealized 1% per year increase in atmospheric CO2 to quadrupling. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 45
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClim4xco2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.2b sstClim4xco2 (6.2b SST Climatology With 4XCO2 Forcing) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice (as in 6.2a) but with quadrupled 4XCO2 imposed. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 46
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. "historicalGHG" is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 7.2 historicalGHG (7.2 GHG-only historical) - Version 1: Historical simulation but with greenhouse gas forcing only. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
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  • 47
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    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. piControl is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.1 piControl (3.1 Pre-Industrial Control) - Version 1: Pre-Industrial coupled atmosphere/ocean control run. Imposes non-evolving pre-industrial conditions. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 48
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'rcp45' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.1 rcp45 (4.1 RCP4.5) - Version 1: Future projection (2006-2100) forced by RCP4.5. RCP4.5 is a representative concentration pathway which approximately results in a radiative forcing of 4.5 W m-2 at year 2100, relative to pre-industrial conditions. RCPs are time-dependent, consistent projections of emissions and concentrations of radiatively active gases and particles. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 49
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. rcp85 is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.2 rcp85 (4.2 RCP8.5) - Version 1: Future projection (2006-2100) forced by RCP8.5. RCP8.5 is a representative concentration pathway which approximately results in a radiative forcing of 8.5 W m-2 at year 2100, relative to pre-industrial conditions. RCPs are time-dependent, consistent projections of emissions and concentrations of radiatively active gases and particles. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 50
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. rcp85 is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.2 rcp85 (4.2 RCP8.5) - Version 1: Future projection (2006-2100) forced by RCP8.5. RCP8.5 is a representative concentration pathway which approximately results in a radiative forcing of 8.5 W m-2 at year 2100, relative to pre-industrial conditions. RCPs are time-dependent, consistent projections of emissions and concentrations of radiatively active gases and particles. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 51
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'rcp45' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.1 rcp45 (4.1 RCP4.5) - Version 1: Future projection (2006-2100) forced by RCP4.5. RCP4.5 is a representative concentration pathway which approximately results in a radiative forcing of 4.5 W m-2 at year 2100, relative to pre-industrial conditions. RCPs are time-dependent, consistent projections of emissions and concentrations of radiatively active gases and particles. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
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  • 52
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'historical' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.2 historical (3.2 Historical) - Version 1: Simulation of recent past (1850 to 2005). Impose changing conditions (consistent with observations). Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
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  • 53
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'historicalNat' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 7.1 historicalNat (7.1 natural-only historical simulation) - Version 1: Historical simulation but with natural forcing only. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 54
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClimSulfate' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.4b sstClimSulfate (6.4b sulfate aerosol forcing) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice (as in expt. 6.2a) but with aerosols consistent with conditions in year 2000 of the historical run (expt. 3.2) Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 55
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'abrupt4xco2' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.3 abrupt4xco2 (6.3 Abrupt 4XCO2) - Version 1: Impose an instantaneous quadrupling of CO2, then hold fixed. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 56
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. rcp85 is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.2 rcp85 (4.2 RCP8.5) - Version 1: Future projection (2006-2100) forced by RCP8.5. RCP8.5 is a representative concentration pathway which approximately results in a radiative forcing of 8.5 W m-2 at year 2100, relative to pre-industrial conditions. RCPs are time-dependent, consistent projections of emissions and concentrations of radiatively active gases and particles. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
    Type: dataset_group
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  • 57
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClimAerosol' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.4a sstClimAerosol (6.4a all aerosol forcing) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice (as in expt. 6.2a) but with aerosols consistent with conditions in year 2000 of the historical run (expt. 3.2) Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 58
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'sstClimSulfate' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 6.4b sstClimSulfate (6.4b sulfate aerosol forcing) - Version 1: AMIP-style experiment with control run climatological SSTs and sea ice (as in expt. 6.2a) but with aerosols consistent with conditions in year 2000 of the historical run (expt. 3.2) Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
    Type: dataset_group
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  • 59
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'amip' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 3.3 amip (3.3 AMIP) - Version 1: AMIP (1979 - at least 2008). Impose SSTs and sea ice from observations but with other conditions as in experiment 3.2 historical. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
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  • 60
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. "historicalGHG" is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 7.2 historicalGHG (7.2 GHG-only historical) - Version 1: Historical simulation but with greenhouse gas forcing only. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
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  • 61
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. rcp26 is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 ( https://pcmdi.llnl.gov/mips/cmip5 ). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.3 rcp26 (4.3 RCP2.6) - Version 1: Future projection (2006-2100) forced by RCP2.6. RCP2.6 is a representative concentration pathway which approximately results in a radiative forcing of 2.6 W m-2 at year 2100, relative to pre-industrial conditions. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax ( https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf ) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc .
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  • 62
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    WDCC
    Publication Date: 2020-10-13
    Description: The experiment includes the latest CMIP5 data of CSIRO for January 2015. The data is a newer version of the IPCC DDC AR5 data of CSIRO. 'rcp60' is an experiment of the CMIP5 - Coupled Model Intercomparison Project Phase 5 (https://pcmdi.llnl.gov/mips/cmip5). CMIP5 is meant to provide a framework for coordinated climate change experiments for the next five years and thus includes simulations for assessment in the AR5 as well as others that extend beyond the AR5. 4.4 rcp60 (4.4 RCP6) - Version 1: Future projection (2006-2100) forced by RCP6. RCP6 is a representative concentration pathway which approximately results in a radiative forcing of 6 W m-2 at year 2100, relative to pre-industrial conditions. Experiment design: https://pcmdi.llnl.gov/mips/cmip5/experiment_design.html List of output variables: https://pcmdi.llnl.gov/mips/cmip5/datadescription.html Output: time series per variable in model grid spatial resolution in netCDF format Earth System model and the simulation information: CIM repository Entry name/title of data are specified according to the Data Reference Syntax (https://pcmdi.llnl.gov/mips/cmip5/docs/cmip5_data_reference_syntax.pdf) as activity/product/institute/model/experiment/frequency/modeling realm/MIP table/ensemble member/version number/variable name/CMOR filename.nc.
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  • 63
    Publication Date: 2015-05-11
    Description: Disruption of circadian rhythmicity is associated with obesity and related disorders, including type 2 diabetes and cardiovascular disease. Specifically, prolonged artificial light exposure associates with obesity in humans, although the underlying mechanism is unclear. Here, we report that increasing the daily hours of light exposure increases body adiposity through attenuation of brown adipose tissue (BAT) activity, a major contributor of energy expenditure. Mice exposed to a prolonged day length of 16- and 24-h light, compared with regular 12-h light, showed increased adiposity without affecting food intake or locomotor activity. Mechanistically, we demonstrated that prolonged day length decreases sympathetic input into BAT and reduces β3-adrenergic intracellular signaling. Concomitantly, prolonging day length decreased the uptake of fatty acids from triglyceride-rich lipoproteins, as well as of glucose from plasma selectively by BAT. We conclude that impaired BAT activity is an important mediator in the association between disturbed circadian rhythm and adiposity, and anticipate that activation of BAT may overcome the adverse metabolic consequences of disturbed circadian rhythmicity.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 64
    Publication Date: 2015-10-26
    Description: The autoactivation of an initiating serine protease upon binding of pattern recognition proteins to pathogen surfaces is a crucial step in eliciting insect immune responses such as the activation of Toll and prophenoloxidase pathways. However, the molecular mechanisms responsible for autoactivation of the initiating protease remains poorly understood. Here, we investigated the molecular basis for the autoactivation of hemolymph protease 14 (HP14), an initiating protease in hemolymph of Manduca sexta, upon the binding of β-1,3-glucan by its recognition protein, βGRP2. Biochemical analysis using HP14 zymogen (proHP14), βGRP2, and the recombinant proteins as truncated forms showed that the amino-terminal modular low-density lipoprotein receptor class A (LA) domains within HP14 are required for proHP14 autoactivation that is stimulated by its interaction with βGRP2. Consistent with this result, recombinant LA domains inhibit the activation of proHP14 and prophenoloxidase, likely by competing with the interaction between βGRP2 and LA domains within proHP14. Using surface plasmon resonance, we demonstrated that immobilized LA domains directly interact with βGRP2 in a calcium-dependent manner and that high-affinity interaction requires the C-terminal glucanase-like domain of βGRP2. Importantly, the affinity of LA domains for βGRP2 increases nearly 100-fold in the presence of β-1,3-glucan. Taken together, these results present the first experimental evidence to our knowledge that LA domains of an insect modular protease and glucanase-like domains of a βGRP mediate their interaction, and that this binding is essential for the protease autoactivation. Thus, our study provides important insight into the molecular basis underlying the initiation of protease cascade in insect immune responses.
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    Topics: Biology , Medicine , Natural Sciences in General
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  • 65
    Publication Date: 2015-12-29
    Description: Synaptic depression is prominent among synapses, but the underlying mechanisms remain uncertain. Here, we use paired patch clamp recording to study neuromuscular transmission between the caudal primary motor neuron and target skeletal muscle in zebrafish. This synapse has an unusually low number of release sites, all with high probabilities of release in response to low-frequency stimulation. During high-frequency stimulation, the synapse undergoes short-term depression and reaches steady-state levels of transmission that sustain the swimming behavior. To determine the release parameters underlying this steady state, we applied variance analysis. Our analysis revealed two functionally distinct subclasses of release sites differing by over 60-fold in rates of vesicle reloading. A slow reloading class requires seconds to recover and contributes to depression onset but not the steady-state transmission. By contrast, a fast reloading class recovers within tens of milliseconds and is solely responsible for steady-state transmission. Thus, in contrast to most current models that assign levels of steady-state depression to vesicle availability, our findings instead assign this function to nonuniform release site kinetics. The duality of active-site properties accounts for the highly nonlinear dependence of steady-state depression levels on frequency.
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    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 66
    Publication Date: 2015-12-23
    Description: Two specific genetic variants of the apolipoprotein L1 (APOL1) gene are responsible for the high rate of kidney disease in people of recent African ancestry. Expression in cultured cells of these APOL1 risk variants, commonly referred to as G1 and G2, results in significant cytotoxicity. The underlying mechanism of this cytotoxicity is poorly understood. We hypothesized that this cytotoxicity is mediated by APOL1 risk variant-induced dysregulation of intracellular signaling relevant for cell survival. To test this hypothesis, we conditionally expressed WT human APOL1 (G0), the APOL1 G1 variant, or the APOL1 G2 variant in human embryonic kidney cells (T-REx-293) using a tetracycline-mediated (Tet-On) system. We found that expression of either G1 or G2 APOL1 variants increased apparent cell swelling and cell death compared with G0-expressing cells. These manifestations of cytotoxicity were preceded by G1 or G2 APOL1-induced net efflux of intracellular potassium as measured by X-ray fluorescence, resulting in the activation of stress-activated protein kinases (SAPKs), p38 MAPK, and JNK. Prevention of net K+ efflux inhibited activation of these SAPKs by APOL1 G1 or G2. Furthermore, inhibition of SAPK signaling and inhibition of net K+ efflux abrogated cytotoxicity associated with expression of APOL1 risk variants. These findings in cell culture raise the possibility that nephrotoxicity of APOL1 risk variants may be mediated by APOL1 risk variant-induced net loss of intracellular K+ and subsequent induction of stress-activated protein kinase pathways.
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  • 67
    Publication Date: 2015-01-26
    Description: G protein-coupled receptor 17 (GPR17) was recently reported to be a Foxo1 target in agouti-related peptide (AGRP) neurons. Intracerebroventricular injection of GPR17 agonists induced food intake, whereas administration of an antagonist to the receptor reduced feeding. These data lead to the conclusion that pharmacological modulation of GPR17 has therapeutic potential to treat obesity. Here we report that mice deficient in Gpr17 (Gpr17−/−) have similar food intake and body weight compared with their wild-type littermates. Gpr17−/− mice have normal hypothalamic Agrp mRNA expression, AGRP plasma levels, and metabolic rate. GPR17 deficiency in mice did not affect glucose homeostasis or prevent fat-induced insulin resistance. These data do not support a role for GPR17 in the control of food intake, body weight, or glycemic control.
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  • 68
    Publication Date: 2015-04-06
    Description: Major changes in bacterial physiology including biofilm and spore formation involve signaling by the cyclic dinucleotides c-di-GMP and c-di-AMP. Recently, another second messenger dinucleotide, c-AMP-GMP, was found to control chemotaxis and colonization by Vibrio cholerae. We have identified a superregulon of genes controlled by c-AMP-GMP in numerous Deltaproteobacteria, including Geobacter species that use extracellular insoluble metal oxides as terminal electron acceptors. This exoelectrogenic process has been studied for its possible utility in energy production and bioremediation. Many genes involved in adhesion, pilin formation, and others that are important for exoelectrogenesis are controlled by members of a variant riboswitch class that selectively bind c-AMP-GMP. These RNAs constitute, to our knowledge, the first known specific receptors for c-AMP-GMP and reveal that this molecule is used by many bacteria to control specialized physiological processes.
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  • 69
    Publication Date: 2015-04-27
    Description: Aminoacyl-tRNA synthetases (ARSs) establish the rules of the genetic code, whereby each amino acid is attached to a cognate tRNA. Errors in this process lead to mistranslation, which can be toxic to cells. The selective forces exerted by species-specific requirements and environmental conditions potentially shape quality-control mechanisms that serve to prevent mistranslation. A family of editing factors that are homologous to the editing domain of bacterial prolyl-tRNA synthetase includes the previously characterized trans-editing factors ProXp-ala and YbaK, which clear Ala-tRNAPro and Cys-tRNAPro, respectively, and three additional homologs of unknown function, ProXp-x, ProXp-y, and ProXp-z. We performed an in vivo screen of 230 conditions in which an Escherichia coli proXp-y deletion strain was grown in the presence of elevated levels of amino acids and specific ARSs. This screen, together with the results of in vitro deacylation assays, revealed Ser- and Thr-tRNA deacylase function for this homolog. A similar activity was demonstrated for Bordetella parapertussis ProXp-z in vitro. These proteins, now renamed “ProXp-ST1” and “ProXp-ST2,” respectively, recognize multiple tRNAs as substrates. Taken together, our data suggest that these free-standing editing domains have the ability to prevent mistranslation errors caused by a number of ARSs, including lysyl-tRNA synthetase, threonyl-tRNA synthetase, seryl-tRNA synthetase, and alanyl-tRNA synthetase. The expression of these multifunctional enzymes is likely to provide a selective growth advantage to organisms subjected to environmental stresses and other conditions that alter the amino acid pool.
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  • 70
    Publication Date: 2015-09-23
    Description: Host compatible rhizobia induce the formation of legume root nodules, symbiotic organs within which intracellular bacteria are present in plant-derived membrane compartments termed symbiosomes. In Medicago truncatula nodules, the Sinorhizobium microsymbionts undergo an irreversible differentiation process leading to the development of elongated polyploid noncultivable nitrogen fixing bacteroids that convert atmospheric dinitrogen into ammonia. This terminal differentiation is directed by the host plant and involves hundreds of nodule specific cysteine-rich peptides (NCRs). Except for certain in vitro activities of cationic peptides, the functional roles of individual NCR peptides in planta are not known. In this study, we demonstrate that the inability of M. truncatula dnf7 mutants to fix nitrogen is due to inactivation of a single NCR peptide, NCR169. In the absence of NCR169, bacterial differentiation was impaired and was associated with early senescence of the symbiotic cells. Introduction of the NCR169 gene into the dnf7-2/NCR169 deletion mutant restored symbiotic nitrogen fixation. Replacement of any of the cysteine residues in the NCR169 peptide with serine rendered it incapable of complementation, demonstrating an absolute requirement for all cysteines in planta. NCR169 was induced in the cell layers in which bacteroid elongation was most pronounced, and high expression persisted throughout the nitrogen-fixing nodule zone. Our results provide evidence for an essential role of NCR169 in the differentiation and persistence of nitrogen fixing bacteroids in M. truncatula.
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  • 71
    Publication Date: 2015-03-30
    Description: Polycystic ovary syndrome (PCOS) is one of the most common female endocrine disorders and a leading cause of female subfertility. The mechanism underlying the pathophysiology of PCOS remains to be illustrated. Here, we identify two alternative splice variants (ASVs) of the androgen receptor (AR), insertion and deletion isoforms, in granulosa cells (GCs) in ∼62% of patients with PCOS. AR ASVs are strongly associated with remarkable hyperandrogenism and abnormalities in folliculogenesis, and are absent from all control subjects without PCOS. Alternative splicing dramatically alters genome-wide AR recruitment and androgen-induced expression of genes related to androgen metabolism and folliculogenesis in human GCs. These findings establish alternative splicing of AR in GCs as the major pathogenic mechanism for hyperandrogenism and abnormal folliculogenesis in PCOS.
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  • 72
    Publication Date: 2015-05-20
    Description: After their endosymbiotic acquisition, plastids become intimately connected with the biology of their host. For example, genes essential for plastid function may be relocated from the genomes of plastids to the host nucleus, and pathways may evolve within the host to support the plastid. In this review, we consider the different degrees of integration observed in dinoflagellates and their associated plastids, which have been acquired through multiple different endosymbiotic events. Most dinoflagellate species possess plastids that contain the pigment peridinin and show extreme reduction and integration with the host biology. In some species, these plastids have been replaced through serial endosymbiosis with plastids derived from a different phylogenetic derivation, of which some have become intimately connected with the biology of the host whereas others have not. We discuss in particular the evolution of the fucoxanthin-containing dinoflagellates, which have adapted pathways retained from the ancestral peridinin plastid symbiosis for transcript processing in their current, serially acquired plastids. Finally, we consider why such a diversity of different degrees of integration between host and plastid is observed in different dinoflagellates and how dinoflagellates may thus inform our broader understanding of plastid evolution and function.
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  • 73
    Publication Date: 2015-04-13
    Description: Toll-like receptor (TLR) signaling is initiated by dimerization of intracellular Toll/IL-1 receptor resistance (TIR) domains. For all TLRs except TLR3, recruitment of the adapter, myeloid differentiation primary response gene 88 (MyD88), to TLR TIR domains results in downstream signaling culminating in proinflammatory cytokine production. Therefore, blocking TLR TIR dimerization may ameliorate TLR2-mediated hyperinflammatory states. The BB loop within the TLR TIR domain is critical for mediating certain protein–protein interactions. Examination of the human TLR2 TIR domain crystal structure revealed a pocket adjacent to the highly conserved P681 and G682 BB loop residues. Using computer-aided drug design (CADD), we sought to identify a small molecule inhibitor(s) that would fit within this pocket and potentially disrupt TLR2 signaling. In silico screening identified 149 compounds and 20 US Food and Drug Administration-approved drugs based on their predicted ability to bind in the BB loop pocket. These compounds were screened in HEK293T-TLR2 transfectants for the ability to inhibit TLR2-mediated IL-8 mRNA. C16H15NO4(C29) was identified as a potential TLR2 inhibitor. C29, and its derivative,ortho-vanillin (o-vanillin), inhibited TLR2/1 and TLR2/6 signaling induced by synthetic and bacterial TLR2 agonists in human HEK-TLR2 and THP-1 cells, but only TLR2/1 signaling in murine macrophages. C29 failed to inhibit signaling induced by other TLR agonists and TNF-α. Mutagenesis of BB loop pocket residues revealed an indispensable role for TLR2/1, but not TLR2/6, signaling, suggesting divergent roles. Mice treated witho-vanillin exhibited reduced TLR2-induced inflammation. Our data provide proof of principle that targeting the BB loop pocket is an effective approach for identification of TLR2 signaling inhibitors.
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  • 74
    Publication Date: 2015-01-20
    Description: The ability to predict the mechanisms and the associated rate constants of protein–ligand unbinding is of great practical importance in drug design. In this work we demonstrate how a recently introduced metadynamics-based approach allows exploration of the unbinding pathways, estimation of the rates, and determination of the rate-limiting steps in the paradigmatic case of the trypsin–benzamidine system. Protein, ligand, and solvent are described with full atomic resolution. Using metadynamics, multiple unbinding trajectories that start with the ligand in the crystallographic binding pose and end with the ligand in the fully solvated state are generated. The unbinding rate koff is computed from the mean residence time of the ligand. Using our previously computed binding affinity we also obtain the binding rate kon. Both rates are in agreement with reported experimental values. We uncover the complex pathways of unbinding trajectories and describe the critical rate-limiting steps with unprecedented detail. Our findings illuminate the role played by the coupling between subtle protein backbone fluctuations and the solvation by water molecules that enter the binding pocket and assist in the breaking of the shielded hydrogen bonds. We expect our approach to be useful in calculating rates for general protein–ligand systems and a valid support for drug design.
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  • 75
    Publication Date: 2015-08-05
    Description: Vitamin B12 (cobalamin) is required by humans and other organisms for diverse metabolic processes, although only a subset of prokaryotes is capable of synthesizing B12 and other cobamide cofactors. The complete aerobic and anaerobic pathways for the de novo biosynthesis of B12 are known, with the exception of the steps leading to the anaerobic biosynthesis of the lower ligand, 5,6-dimethylbenzimidazole (DMB). Here, we report the identification and characterization of the complete pathway for anaerobic DMB biosynthesis. This pathway, identified in the obligate anaerobic bacterium Eubacterium limosum, is composed of five previously uncharacterized genes, bzaABCDE, that together direct DMB production when expressed in anaerobically cultured Escherichia coli. Expression of different combinations of the bza genes revealed that 5-hydroxybenzimidazole, 5-methoxybenzimidazole, and 5-methoxy-6-methylbenzimidazole, all of which are lower ligands of cobamides produced by other organisms, are intermediates in the pathway. The bza gene content of several bacterial and archaeal genomes is consistent with experimentally determined structures of the benzimidazoles produced by these organisms, indicating that these genes can be used to predict cobamide structure. The identification of the bza genes thus represents the last remaining unknown component of the biosynthetic pathway for not only B12 itself, but also for three other cobamide lower ligands whose biosynthesis was previously unknown. Given the importance of cobamides in environmental, industrial, and human-associated microbial metabolism, the ability to predict cobamide structure may lead to an improved ability to understand and manipulate microbial metabolism.
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  • 76
    Publication Date: 2015-12-07
    Description: Published estimates of methane emissions from atmospheric data (top-down approaches) exceed those from source-based inventories (bottom-up approaches), leading to conflicting claims about the climate implications of fuel switching from coal or petroleum to natural gas. Based on data from a coordinated campaign in the Barnett Shale oil and gas-producing region of Texas, we find that top-down and bottom-up estimates of both total and fossil methane emissions agree within statistical confidence intervals (relative differences are 10% for fossil methane and 0.1% for total methane). We reduced uncertainty in top-down estimates by using repeated mass balance measurements, as well as ethane as a fingerprint for source attribution. Similarly, our bottom-up estimate incorporates a more complete count of facilities than past inventories, which omitted a significant number of major sources, and more effectively accounts for the influence of large emission sources using a statistical estimator that integrates observations from multiple ground-based measurement datasets. Two percent of oil and gas facilities in the Barnett accounts for half of methane emissions at any given time, and high-emitting facilities appear to be spatiotemporally variable. Measured oil and gas methane emissions are 90% larger than estimates based on the US Environmental Protection Agency’s Greenhouse Gas Inventory and correspond to 1.5% of natural gas production. This rate of methane loss increases the 20-y climate impacts of natural gas consumed in the region by roughly 50%.
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  • 77
    Publication Date: 2015-04-09
    Description: Transmembrane β-barrels (TMBs) carry out major functions in substrate transport and protein biogenesis but experimental determination of their 3D structure is challenging. Encouraged by successful de novo 3D structure prediction of globular and α-helical membrane proteins from sequence alignments alone, we developed an approach to predict the 3D structure of TMBs. The approach combines the maximum-entropy evolutionary coupling method for predicting residue contacts (EVfold) with a machine-learning approach (boctopus2) for predicting β-strands in the barrel. In a blinded test for 19 TMB proteins of known structure that have a sufficient number of diverse homologous sequences available, this combined method (EVfold_bb) predicts hydrogen-bonded residue pairs between adjacent β-strands at an accuracy of ∼70%. This accuracy is sufficient for the generation of all-atom 3D models. In the transmembrane barrel region, the average 3D structure accuracy [template-modeling (TM) score] of top-ranked models is 0.54 (ranging from 0.36 to 0.85), with a higher (44%) number of residue pairs in correct strand–strand registration than in earlier methods (18%). Although the nonbarrel regions are predicted less accurately overall, the evolutionary couplings identify some highly constrained loop residues and, for FecA protein, the barrel including the structure of a plug domain can be accurately modeled (TM score = 0.68). Lower prediction accuracy tends to be associated with insufficient sequence information and we therefore expect increasing numbers of β-barrel families to become accessible to accurate 3D structure prediction as the number of available sequences increases.
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  • 78
    Publication Date: 2015-12-11
    Description: Preeclampsia (PE) is a pregnancy-specific disorder characterized by hypertension and proteinuria after 20 wk gestation. Abnormal extravillous trophoblast (EVT) invasion and remodeling of uterine spiral arterioles is thought to contribute to PE development. Interleukin-11 (IL11) impedes human EVT invasion in vitro and is elevated in PE decidua in women. We demonstrate that IL11 administered to mice causes development of PE features. Immunohistochemistry shows IL11 compromises trophoblast invasion, spiral artery remodeling, and placentation, leading to increased systolic blood pressure (SBP), proteinuria, and intrauterine growth restriction, although nonpregnant mice were unaffected. Real-time PCR array analysis identified pregnancy-associated plasma protein A2 (PAPPA2), associated with PE in women, as an IL11 regulated target. IL11 increased PAPPA2 serum and placental tissue levels in mice. In vitro, IL11 compromised primary human EVT invasion, whereas siRNA knockdown of PAPPA2 alleviated the effect. Genes regulating uterine natural killer (uNK) recruitment and differentiation were down-regulated and uNK cells were reduced after IL11 treatment in mice. IL11 withdrawal in mice at onset of PE features reduced SBP and proteinuria to control levels and alleviated placental labyrinth defects. In women, placental IL11 immunostaining levels increased in PE pregnancies and in serum collected from women before development of early-onset PE, shown by ELISA. These results indicate that elevated IL11 levels result in physiological changes at the maternal–fetal interface, contribute to abnormal placentation, and lead to the development of PE. Targeting placental IL11 may provide a new treatment option for PE.
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  • 79
    Publication Date: 2015-09-23
    Description: We previously computed that genes with de novo (DN) likely gene-disruptive (LGD) mutations in children with autism spectrum disorders (ASD) have high vulnerability: disruptive mutations in many of these genes, the vulnerable autism genes, will have a high likelihood of resulting in ASD. Because individuals with ASD have lower fecundity, such mutations in autism genes would be under strong negative selection pressure. An immediate prediction is that these genes will have a lower LGD load than typical genes in the human gene pool. We confirm this hypothesis in an explicit test by measuring the load of disruptive mutations in whole-exome sequence databases from two cohorts. We use information about mutational load to show that lower and higher intelligence quotients (IQ) affected individuals can be distinguished by the mutational load in their respective gene targets, as well as to help prioritize gene targets by their likelihood of being autism genes. Moreover, we demonstrate that transmission of rare disruptions in genes with a lower LGD load occurs more often to affected offspring; we show transmission originates most often from the mother, and transmission of such variants is seen more often in offspring with lower IQ. A surprising proportion of transmission of these rare events comes from genes expressed in the embryonic brain that show sharply reduced expression shortly after birth.
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  • 80
    Publication Date: 2015-11-30
    Description: Dynamic particle-scale numerical simulations are used to show that the shear thickening observed in dense colloidal, or Brownian, suspensions is of a similar nature to that observed in noncolloidal suspensions, i.e., a stress-induced transition from a flow of lubricated near-contacting particles to a flow of a frictionally contacting network of particles. Abrupt (or discontinuous) shear thickening is found to be a geometric rather than hydrodynamic phenomenon; it stems from the strong sensitivity of the jamming volume fraction to the nature of contact forces between suspended particles. The thickening obtained in a colloidal suspension of purely hard frictional spheres is qualitatively similar to experimental observations. However, the agreement cannot be made quantitative with only hydrodynamics, frictional contacts, and Brownian forces. Therefore, the role of a short-range repulsive potential mimicking the stabilization of actual suspensions on the thickening is studied. The effects of Brownian and repulsive forces on the onset stress can be combined in an additive manner. The simulations including Brownian and stabilizing forces show excellent agreement with experimental data for the viscosity η and the second normal stress difference N2.
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  • 81
    Publication Date: 2015-10-02
    Description: Classical feed-forward inhibition involves an excitation–inhibition sequence that enhances the temporal precision of neuronal responses by narrowing the window for synaptic integration. In the input layer of the cerebellum, feed-forward inhibition is thought to preserve the temporal fidelity of granule cell spikes during mossy fiber stimulation. Although this classical feed-forward inhibitory circuit has been demonstrated in vitro, the extent to which inhibition shapes granule cell sensory responses in vivo remains unresolved. Here we combined whole-cell patch-clamp recordings in vivo and dynamic clamp recordings in vitro to directly assess the impact of Golgi cell inhibition on sensory information transmission in the granule cell layer of the cerebellum. We show that the majority of granule cells in Crus II of the cerebrocerebellum receive sensory-evoked phasic and spillover inhibition prior to mossy fiber excitation. This preceding inhibition reduces granule cell excitability and sensory-evoked spike precision, but enhances sensory response reproducibility across the granule cell population. Our findings suggest that neighboring granule cells and Golgi cells can receive segregated and functionally distinct mossy fiber inputs, enabling Golgi cells to regulate the size and reproducibility of sensory responses.
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  • 82
    Publication Date: 2015-04-20
    Description: Model projections indicate that climate change may dramatically restructure phytoplankton communities, with cascading consequences for marine food webs. It is currently not known whether evolutionary change is likely to be able to keep pace with the rate of climate change. For simplicity, and in the absence of evidence to the contrary, most model projections assume species have fixed environmental preferences and will not adapt to changing environmental conditions on the century scale. Using 15 y of observations from Station CARIACO (Carbon Retention in a Colored Ocean), we show that most of the dominant species from a marine phytoplankton community were able to adapt their realized niches to track average increases in water temperature and irradiance, but the majority of species exhibited a fixed niche for nitrate. We do not know the extent of this adaptive capacity, so we cannot conclude that phytoplankton will be able to adapt to the changes anticipated over the next century, but community ecosystem models can no longer assume that phytoplankton cannot adapt.
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  • 83
    Publication Date: 2015-05-18
    Description: Guiding behavior requires the brain to make predictions about the future values of sensory inputs. Here, we show that efficient predictive computation starts at the earliest stages of the visual system. We compute how much information groups of retinal ganglion cells carry about the future state of their visual inputs and show that nearly every cell in the retina participates in a group of cells for which this predictive information is close to the physical limit set by the statistical structure of the inputs themselves. Groups of cells in the retina carry information about the future state of their own activity, and we show that this information can be compressed further and encoded by downstream predictor neurons that exhibit feature selectivity that would support predictive computations. Efficient representation of predictive information is a candidate principle that can be applied at each stage of neural computation.
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  • 84
    Publication Date: 2015-09-08
    Description: Acanthamoeba species are infected by the largest known DNA viruses. These include icosahedral Mimiviruses, amphora-shaped Pandoraviruses, and Pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. Mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. Its approximately spherical virion (0.6-µm diameter) encloses a 651-kb GC-rich genome encoding 523 proteins of which 64% are ORFans; 16% have their closest homolog in Pandoraviruses and 10% in Acanthamoeba castellanii probably through horizontal gene transfer. The Mollivirus nucleocytoplasmic replication cycle was analyzed using a combination of “omic” approaches that revealed how the virus highjacks its host machinery to actively replicate. Surprisingly, the host’s ribosomal proteins are packaged in the virion. Metagenomic analysis of the permafrost sample uncovered the presence of both viruses, yet in very low amount. The fact that two different viruses retain their infectivity in prehistorical permafrost layers should be of concern in a context of global warming. Giant viruses’ diversity remains to be fully explored.
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  • 85
    Publication Date: 2015-04-14
    Description: Inositol 1,4,5-trisphosphate receptor (IP3R) binding protein released with IP3 (IRBIT) contributes to various physiological events (electrolyte transport and fluid secretion, mRNA polyadenylation, and the maintenance of genomic integrity) through its interaction with multiple targets. However, little is known about the physiological role of IRBIT in the brain. Here we identified calcium calmodulin-dependent kinase II alpha (CaMKIIα) as an IRBIT-interacting molecule in the central nervous system. IRBIT binds to and suppresses CaMKIIα kinase activity by inhibiting the binding of calmodulin to CaMKIIα. In addition, we show that mice lacking IRBIT present with elevated catecholamine levels, increased locomotor activity, and social abnormalities. The level of tyrosine hydroxylase (TH) phosphorylation by CaMKIIα, which affects TH activity, was significantly increased in the ventral tegmental area of IRBIT-deficient mice. We concluded that IRBIT suppresses CaMKIIα activity and contributes to catecholamine homeostasis through TH phosphorylation.
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  • 86
    Publication Date: 2015-01-05
    Description: The two products of the KRAS locus, K-Ras4A and K-Ras4B, are encoded by alternative fourth exons and therefore, possess distinct membrane-targeting sequences. The common activating mutations occur in exons 1 or 2 and therefore, render both splice variants oncogenic. K-Ras4A has been understudied, because it has been considered a minor splice variant. By priming off of the splice junction, we developed a quantitative RT-PCR assay for K-Ras4A and K-Ras4B message capable of measuring absolute amounts of the two transcripts. We found that K-Ras4A was widely expressed in 30 of 30 human cancer cell lines and amounts equal to K-Ras4B in 17 human colorectal tumors. Using splice variant-specific antibodies, we detected K-Ras4A protein in several tumor cell lines at a level equal to or greater than that of K-Ras4B. In addition to the CAAX motif, the C terminus of K-Ras4A contains a site of palmitoylation as well as a bipartite polybasic region. Although both were required for maximal efficiency, each of these could independently deliver K-Ras4A to the plasma membrane. Thus, among four Ras proteins, K-Ras4A is unique in possessing a dual membrane-targeting motif. We also found that, unlike K-Ras4B, K-Ras4A does not bind to the cytosolic chaperone δ-subunit of cGMP phosphodiesterase type 6 (PDE6δ). We conclude that efforts to develop anti–K-Ras drugs that interfere with membrane trafficking will have to take into account the distinct modes of targeting of the two K-Ras splice variants.
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  • 87
    Publication Date: 2015-11-20
    Description: Coherent laser radiation has enabled many scientific and technological breakthroughs including Bose–Einstein condensates, ultrafast spectroscopy, superresolution optical microscopy, photothermal therapy, and long-distance telecommunications. However, it has remained a challenge to refrigerate liquid media (including physiological buffers) during laser illumination due to significant background solvent absorption and the rapid (∼ps) nonradiative vibrational relaxation of molecular electronic excited states. Here we demonstrate that single-beam laser trapping can be used to induce and quantify the local refrigeration of physiological media by 〉10 °C following the emission of photoluminescence from upconverting yttrium lithium fluoride (YLF) nanocrystals. A simple, low-cost hydrothermal approach is used to synthesize polycrystalline particles with sizes ranging from 1 μm. A tunable, near-infrared continuous-wave laser is used to optically trap individual YLF crystals with an irradiance on the order of 1 MW/cm2. Heat is transported out of the crystal lattice (across the solid–liquid interface) by anti-Stokes (blue-shifted) photons following upconversion of Yb3+ electronic excited states mediated by the absorption of optical phonons. Temperatures are quantified through analysis of the cold Brownian dynamics of individual nanocrystals in an inhomogeneous temperature field via forward light scattering in the back focal plane. The cold Brownian motion (CBM) analysis of individual YLF crystals indicates local cooling by 〉21 °C below ambient conditions in D2O, suggesting a range of potential future applications including single-molecule biophysics and integrated photonic, electronic, and microfluidic devices.
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  • 88
    Publication Date: 2015-08-18
    Description: Functional connectivity between the hippocampus and prefrontal cortex (PFC) is essential for associative recognition memory and working memory. Disruption of hippocampal–PFC synchrony occurs in schizophrenia, which is characterized by hypofunction of NMDA receptor (NMDAR)-mediated transmission. We demonstrate that activity of dopamine D2-like receptors (D2Rs) leads selectively to long-term depression (LTD) of hippocampal–PFC NMDAR-mediated synaptic transmission. We show that dopamine-dependent LTD of NMDAR-mediated transmission profoundly disrupts normal synaptic transmission between hippocampus and PFC. These results show how dopaminergic activation induces long-term hypofunction of NMDARs, which can contribute to disordered functional connectivity, a characteristic that is a hallmark of psychiatric disorders such as schizophrenia.
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  • 89
    Publication Date: 2015-08-31
    Description: Although anatomy is often the first step in assigning functions to neural structures, it is not always clear whether architecturally distinct regions of the brain correspond to operational units. Whereas neuroarchitecture remains relatively static, functional connectivity may change almost instantaneously according to behavioral context. We imaged panneuronal responses to visual stimuli in a highly conserved central brain region in the fruit fly, Drosophila, during flight. In one substructure, the fan-shaped body, automated analysis revealed three layers that were unresponsive in quiescent flies but became responsive to visual stimuli when the animal was flying. The responses of these regions to a broad suite of visual stimuli suggest that they are involved in the regulation of flight heading. To identify the cell types that underlie these responses, we imaged activity in sets of genetically defined neurons with arborizations in the targeted layers. The responses of this collection during flight also segregated into three sets, confirming the existence of three layers, and they collectively accounted for the panneuronal activity. Our results provide an atlas of flight-gated visual responses in a central brain circuit.
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  • 90
    Publication Date: 2015-11-17
    Description: We surveyed the “dark” proteome–that is, regions of proteins never observed by experimental structure determination and inaccessible to homology modeling. For 546,000 Swiss-Prot proteins, we found that 44–54% of the proteome in eukaryotes and viruses was dark, compared with only ∼14% in archaea and bacteria. Surprisingly, most of the dark proteome could not be accounted for by conventional explanations, such as intrinsic disorder or transmembrane regions. Nearly half of the dark proteome comprised dark proteins, in which the entire sequence lacked similarity to any known structure. Dark proteins fulfill a wide variety of functions, but a subset showed distinct and largely unexpected features, such as association with secretion, specific tissues, the endoplasmic reticulum, disulfide bonding, and proteolytic cleavage. Dark proteins also had short sequence length, low evolutionary reuse, and few known interactions with other proteins. These results suggest new research directions in structural and computational biology.
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  • 91
    Publication Date: 2015-05-19
    Description: We predict by first-principles methods a phase transition in TiO2 at 6.5 Mbar from the Fe2P-type polymorph to a ten-coordinated structure with space group I4/mmm. This is the first report, to our knowledge, of the pressure-induced phase transition to the I4/mmm structure among all dioxide compounds. The I4/mmm structure was found to be up to 3.3% denser across all pressures investigated. Significant differences were found in the electronic properties of the two structures, and the metallization of TiO2 was calculated to occur concomitantly with the phase transition to I4/mmm. The implications of our findings were extended to SiO2, and an analogous Fe2P-type to I4/mmm transition was found to occur at 10 TPa. This is consistent with the lower-pressure phase transitions of TiO2, which are well-established models for the phase transitions in other AX2 compounds, including SiO2. As in TiO2, the transition to I4/mmm corresponds to the metallization of SiO2. This transformation is in the pressure range reached in the interiors of recently discovered extrasolar planets and calls for a reformulation of the equations of state used to model them.
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  • 92
    Publication Date: 2015-05-18
    Description: Decomposition of organic material by soil microbes generates an annual global release of 50–75 Pg carbon to the atmosphere, ∼7.5–9 times that of anthropogenic emissions worldwide. This process is sensitive to global change factors, which can drive carbon cycle–climate feedbacks with the potential to enhance atmospheric warming. Although the effects of interacting global change factors on soil microbial activity have been a widespread ecological focus, the regulatory effects of interspecific interactions are rarely considered in climate feedback studies. We explore the potential of soil animals to mediate microbial responses to warming and nitrogen enrichment within a long-term, field-based global change study. The combination of global change factors alleviated the bottom-up limitations on fungal growth, stimulating enzyme production and decomposition rates in the absence of soil animals. However, increased fungal biomass also stimulated consumption rates by soil invertebrates, restoring microbial process rates to levels observed under ambient conditions. Our results support the contemporary theory that top-down control in soil food webs is apparent only in the absence of bottom-up limitation. As such, when global change factors alleviate the bottom-up limitations on microbial activity, top-down control becomes an increasingly important regulatory force with the capacity to dampen the strength of positive carbon cycle–climate feedbacks.
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  • 93
    Publication Date: 2015-10-22
    Description: Glutamine synthetase (GS), a key enzyme in biological nitrogen assimilation, is regulated in multiple ways in response to varying nitrogen sources and levels. Here we show a small regulatory RNA, NsiR4 (nitrogen stress-induced RNA 4), which plays an important role in the regulation of GS in cyanobacteria. NsiR4 expression in the unicellularSynechocystissp. PCC 6803 and in the filamentous, nitrogen-fixingAnabaenasp. PCC 7120 is stimulated through nitrogen limitation via NtcA, the global transcriptional regulator of genes involved in nitrogen metabolism. NsiR4 is widely conserved throughout the cyanobacterial phylum, suggesting a conserved function. In silico target prediction, transcriptome profiling on pulse overexpression, and site-directed mutagenesis experiments using a heterologous reporter system showed that NsiR4 interacts with the 5′UTR ofgifAmRNA, which encodes glutamine synthetase inactivating factor (IF)7. InSynechocystis, we observed an inverse relationship between the levels of NsiR4 and the accumulation of IF7 in vivo. This NsiR4-dependent modulation ofgifA(IF7) mRNA accumulation influenced the glutamine pool and thusNH4+assimilation via GS. As a second target, we identifiedssr1528, a hitherto uncharacterized nitrogen-regulated gene. Competition experiments between WT and an ΔnsiR4KO mutant showed that the lack of NsiR4 led to decreased acclimation capabilities ofSynechocystistoward oscillating nitrogen levels. These results suggest a role for NsiR4 in the regulation of nitrogen metabolism in cyanobacteria, especially for the adaptation to rapid changes in available nitrogen sources and concentrations. NsiR4 is, to our knowledge, the first identified bacterial sRNA regulating the primary assimilation of a macronutrient.
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  • 94
    Publication Date: 2015-03-02
    Description: Bone mass is maintained by balanced activity of osteoblasts and osteoclasts. Lrp4 (low-density lipoprotein receptor related protein 4) is a member of the LDL receptor family, whose mutations have been identified in patients with high–bone-mass disorders, such as sclerosteosis and van Buchem diseases. However, it remains unknown whether and how Lrp4 regulates bone-mass homeostasis in vivo. Here we provide evidence that Lrp4-null mutation or specific mutation in osteoblast-lineage cells increased cortical and trabecular bone mass, which was associated with elevated bone formation and impaired bone resorption. This phenotype was not observed in osteoclast-selective Lrp4 knockout mice. Mechanistic studies indicate that loss of Lrp4 function in osteoblast-lineage cells increased serum levels of sclerostin, a key factor for bone-mass homeostasis that interacts with Lrp4, but abolished the inhibition of Wnt/β-catenin signaling and osteoblastic differentiation by sclerostin. Concomitantly, sclerostin induction of RANKL (receptor activator of nuclear kappa B ligand) was impaired, leading to a lower ratio of RANKL over OPG (osteoprotegerin) (a key factor for osteoclastogenesis). Taken together, these results support the view for Lrp4 as a receptor of sclerostin to inhibit Wnt/β-catenin signaling and bone formation and identify Lrp4 as a critical player in bone-mass homeostasis.
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  • 95
    Publication Date: 2015-01-23
    Description: Methane emissions from natural gas delivery and end use must be quantified to evaluate the environmental impacts of natural gas and to develop and assess the efficacy of emission reduction strategies. We report natural gas emission rates for 1 y in the urban region of Boston, using a comprehensive atmospheric measurement and modeling framework. Continuous methane observations from four stations are combined with a high-resolution transport model to quantify the regional average emission flux, 18.5 ± 3.7 (95% confidence interval) g CH4⋅m−2⋅y−1. Simultaneous observations of atmospheric ethane, compared with the ethane-to-methane ratio in the pipeline gas delivered to the region, demonstrate that natural gas accounted for ∼60–100% of methane emissions, depending on season. Using government statistics and geospatial data on natural gas use, we find the average fractional loss rate to the atmosphere from all downstream components of the natural gas system, including transmission, distribution, and end use, was 2.7 ± 0.6% in the Boston urban region, with little seasonal variability. This fraction is notably higher than the 1.1% implied by the most closely comparable emission inventory.
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  • 96
    Publication Date: 2015-11-16
    Description: microRNAs (miRNAs) can act as oncosuppressors or oncogenes, induce chemoresistance or chemosensitivity, and are major posttranscriptional gene regulators. Anaplastic lymphoma kinase (ALK), EGF receptor (EGFR), and V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) are major drivers of non-small cell lung cancer (NSCLC). The aim of this study was to assess the miRNA profiles of NSCLCs driven by translocated ALK, mutant EGFR, or mutant KRAS to find driver-specific diagnostic and prognostic miRNA signatures. A total of 85 formalin-fixed, paraffin-embedded samples were considered: 67 primary NSCLCs and 18 matched normal lung tissues. Of the 67 primary NSCLCs, 17 were echinoderm microtubule-associated protein-like 4–ALK translocated (ALK+) lung cancers; the remaining 50 were not (ALK−). Of the 50 ALK− primary NSCLCs, 24 were EGFR and KRAS mutation-negative (i.e., WT; triple negative); 11 were mutant EGFR (EGFR+), and 15 were mutant KRAS (KRAS+). We developed a diagnostic classifier that shows how miR-1253, miR-504, and miR-26a-5p expression levels can classify NSCLCs as ALK-translocated, mutant EGFR, or mutant KRAS versus mutation-free. We also generated a prognostic classifier based on miR-769-5p and Let-7d-5p expression levels that can predict overall survival. This classifier showed better performance than the commonly used classifiers based on mutational status. Although it has several limitations, this study shows that miRNA signatures and classifiers have great potential as powerful, cost-effective next-generation tools to improve and complement current genetic tests. Further studies of these miRNAs can help define their roles in NSCLC biology and in identifying best-performing chemotherapy regimens.
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  • 97
    Publication Date: 2015-04-13
    Description: Human pluripotent stem cells (PSCs) show epiblast-type pluripotency that is maintained with ACTIVIN/FGF2 signaling. Here, we report the acquisition of a unique stem cell phenotype by both human ES cells (hESCs) and induced pluripotent stem cells (iPSCs) in response to transient (24–36 h) exposure to bone morphogenetic protein 4 (BMP4) plus inhibitors of ACTIVIN signaling (A83-01) and FGF2 (PD173074), followed by trypsin dissociation and recovery of colonies capable of growing on a gelatin substratum in standard medium for human PSCs at low but not high FGF2 concentrations. The self-renewing cell lines stain weakly for CDX2 and strongly for NANOG, can be propagated clonally on either Matrigel or gelatin, and are morphologically distinct from human PSC progenitors on either substratum but still meet standard in vitro criteria for pluripotency. They form well-differentiated teratomas in immune-compromised mice that secrete human chorionic gonadotropin (hCG) into the host mouse and include small areas of trophoblast-like cells. The cells have a distinct transcriptome profile from the human PSCs from which they were derived (including higher expression of NANOG, LEFTY1, and LEFTY2). In nonconditioned medium lacking FGF2, the colonies spontaneously differentiated along multiple lineages, including trophoblast. They responded to PD173074 in the absence of both FGF2 and BMP4 by conversion to trophoblast, and especially syncytiotrophoblast, whereas an A83-01/PD173074 combination favored increased expression of HLA-G, a marker of extravillous trophoblast. Together, these data suggest that the cell lines exhibit totipotent potential and that BMP4 can prime human PSCs to a self-renewing alternative state permissive for trophoblast development. The results may have implications for regulation of lineage decisions in the early embryo.
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  • 98
    Publication Date: 2015-09-15
    Description: Light-induced material phase transitions enable the formation of shapes and patterns from the nano- to the macroscale. From lithographic techniques that enable high-density silicon circuit integration, to laser cutting and welding, light–matter interactions are pervasive in everyday materials fabrication and transformation. These noncontact patterning techniques are ideally suited to reshape soft materials of biological relevance. We present here the use of relatively low-energy (
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  • 99
    Publication Date: 2015-02-23
    Description: Even moderate doses of alcohol cause considerable impairment of motor coordination, an effect that substantially involves potentiation of GABAergic activity at δ subunit-containing GABAA receptors (δ-GABAARs). Here, we demonstrate that oxytocin selectively attenuates ethanol-induced motor impairment and ethanol-induced increases in GABAergic activity at δ-GABAARs and that this effect does not involve the oxytocin receptor. Specifically, oxytocin (1 µg i.c.v.) given before ethanol (1.5 g/kg i.p.) attenuated the sedation and ataxia induced by ethanol in the open-field locomotor test, wire-hanging test, and righting-reflex test in male rats. Using two-electrode voltage-clamp electrophysiology in Xenopus oocytes, oxytocin was found to completely block ethanol-enhanced activity at α4β1δ and α4β3δ recombinant GABAARs. Conversely, ethanol had no effect when applied to α4β1 or α4β3 cells, demonstrating the critical presence of the δ subunit in this effect. Oxytocin had no effect on the motor impairment or in vitro effects induced by the δ-selective GABAAR agonist 4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol, which binds at a different site on δ-GABAARs than ethanol. Vasopressin, which is a nonapeptide with substantial structural similarity to oxytocin, did not alter ethanol effects at δ-GABAARs. This pattern of results confirms the specificity of the interaction between oxytocin and ethanol at δ-GABAARs. Finally, our in vitro constructs did not express any oxytocin receptors, meaning that the observed interactions occur directly at δ-GABAARs. The profound and direct interaction observed between oxytocin and ethanol at the behavioral and cellular level may have relevance for the development of novel therapeutics for alcohol intoxication and dependence.
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  • 100
    Publication Date: 2015-03-02
    Description: The uterine epithelium of mice and humans undergoes cyclical waves of cell proliferation and differentiation under the regulation of estradiol-17β (E2) and progesterone (P4). These epithelial cells respond to E2 with increased protein and DNA synthesis, whereas P4 inhibits only the E2-induced DNA synthetic response. Here we show that E2 regulates protein synthesis in these epithelial cells through activating PKC that in turn stimulates ERK1/2 to phosphorylate and thereby activate the central regulator of protein synthesis mechanistic target of rapamycin (mTOR). This mTOR pathway is not inhibited by P4. Inhibitor studies with an estrogen receptor (ESR1) antagonist showed the dependence of this mTOR pathway on ESR1 but that once activated, a phosphorylation cascade independent of ESR1 propagates the pathway. E2 also stimulates an IGF1 receptor (IGF1R) to PI3 kinase to AKT to GSK-3β pathway required for activation of the canonical cell cycle machinery that is inhibited by P4. PKC activation did not stimulate this pathway nor does inhibition of PKC or ERK1/2 affect it. These studies therefore indicate a mechanism whereby DNA and protein synthesis are regulated by two ESR1-activated pathways that run in parallel with only the one responsible for the initiation of DNA synthesis blocked by P4. Inhibition of mTOR by rapamycin in vivo resulted in inhibition of E2-induced protein and DNA synthesis. Proliferative diseases of the endometrium such as endometriosis and cancer are common and E2 dependent. Thus, defining this mTOR pathway suggests that local (intrauterine or peritoneal) rapamycin administration might be a therapeutic option for these diseases.
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