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  • General Chemistry  (14,839)
  • Cell & Developmental Biology  (7,335)
  • Limnology
  • 2015-2019  (14)
  • 1985-1989  (10,313)
  • 1975-1979  (5,781)
  • 1945-1949  (1,246)
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  • 1
    Publication Date: 2021-05-19
    Description: Aquaculture activity is restricted mainly to rainbow trout in cold waters and carps in warm waters in inland waters of Iran as well as Fars Province. Probably for this reason saline waters of south and southeast Fars have not been used for aquaculture. These rivers are ecosystems with a diverse biota, it is predictable that can be found some there endemic aquatic organisms with a potential for aquaculture. Finding a suitable organism can help increase wise use of this natural resource, and local production and recruitment. However, it is highly needed to investigate various environmental elements prior to any use to obtain an estimation of the environmental consequences, parallel to finding usage potentials. In This study, we investigated Dehram saline river in south of Fars Province. Hydrological and physiographical properties, chemical composition of the river, and aquatic populations were sampled and measured monthly in 3 stations in one year. Salinity, electrical conductivity, and total dissolved solids increased from station 1 to 3, reaching from 6.6 g/L, 14.3 g/L, and 12800 µs/cm to 13.5 g/L, 17.7 g/L, and 16500 µs/cm, respectively. Dissolved oxygen and oxygen saturation were higher in station 3, and in autumn and winter. Nitrite showed lowest values in winter (0.006 mg/L) and highest values in spring (0.013 mg/L). Nitrate showed the lowest concentration in station 3 (0.43 mg/L), but no differences were seen among seasons. Ammonium showed no differences among stations or seasons. Phosphate levels in station 1 and 3 were 0.13 mg/L‌to 0.17 mg/L, with the highest level (0.29 mg/L) seen in the spring. Five species of fishes were identified in the river, which all were widely favored by local people. Some of these have been categorized as edible in previous studies, others as ornamental. On the other hand, intensive growth of an algal species throughout the river demonstrates a potential for algal cultivation. Significant role of algae in production of food for both man and livestock is now well recognized in aquaculture industry.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecology ; Dehram River ; Limnology ; Saline water ; Physico-chemical parameters ; Aquaculture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Limnological factors of brackish water pounds of the rainbow trout, Onchorhynchus mykiss were studied at Bafgh region in Yazd province. Physicochemical factors, phytoplankton, zooplankton and benthic populations of pools, supply and drainage channels from different farms were studied in aquaculture cycle in 2002 and 2003. Means were compared using Tukey HSD and t tests. Meanings of factors in 2002 and 2003 were respectively: and 9.3 0.2ppt; dissolved oxygen 7.9 0.2mg/l water temperature 13.8 0.3°c and 14.9 0.6°c; salinity 12.3 0.2ppt and 7.2 0.1mg/l; pH between 7.8 to 9.2 and 8.1 to 9.5; depth of hyalinity 0.85 0.1m and 1.1 0.1m; ammonium 0.1-0.5mg/l; and nitrate 1.8-21mg/l. Main phytoplankton were diatoms specially Chaetoceros, and Cyanophyta. Rotifera were the dominant zooplankton. Benthic fauna mainly included immature Chironomids. Final mean lengths were between 24.7cm to 31.5cm, mean weights between 11179.9gr to 434.6gr. FCR between 1.16 to 1.6; DGR between 1.04gr/day to 3.03gr/day; production 0.03kg/m2 to 0.84 kg/m2. Supply channels had the optimum limnological condition for trout aquaculture. Limnological factors were often not significantly different between pools and drainage channels. Saeeid and Rangin-Kaman farms were the most successful farms.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Limnology ; Brackish water ; Onchorhynchus mykiss ; Rainbow trout ; Phytoplankton ; Zooplankton ; Chaetoceros ; Survey ; Pond culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 175pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Gahar-roud is in 18 kilometer far from Doroud city from East of lorestan province . This river orginated from internal part of Oshteran-kouh mountain altitude and Gahar-Lake . Survey on Gahar roud Limnology began from the end of 2001 year and during the 18 month achived, at first on the length of 20 kilometer five station of sampling was selected and biological, physical and chemical character of this river was surveyed this river in all path of it feeding with large and small spring, little rivers and water of icehouse. The amount water of this river is different and maximum water is about 21 m/s in April month. Specifity of this river is be enough the temperature of water for fish. Minimum in the Jan is about 3 centigrad deegree and maximum is July and Aug about 24 centigrade deegree phis between 6.5-8.2 and is few basic . solauble oxigen is enough and is between 8.5-13 mg/lit.during the year.This river is very bright and haven t the source of polution and during the path of river and be enaugh the dosage of mineral in water are the important physical and chemical character of this river. Gahar-roud is not biological hardness quality. About 31 genus and species from 5 family of phytoplangton and 42 genus from 26 family from the order of insects, molusca, worms and crustacea and 9 species from 12 family from the side aquatic. Plants was collected and recognized. Based on ecological potential of this region, because Gahar- roud river is poor for food material and classified in oligotroph river and hasn t high biological value. And also have a few population and in fact haven t any population and based on the safer system belong to the quatiy elass of oligotroph and amount of water, regular circulation of water with microclima condition rate of natural resource land around river, having program for culture of cold water fish is important but it is better that use from culture of fish, be regulated key word limnology, Gahar-roud, river plangton, bentoz, fish.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Limnology ; Fish ; Bentos ; Plankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 84pp.
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  • 4
    Publication Date: 2021-05-19
    Description: Dorudzan reservoir is the main provider of drinking water for the capital Shiraz, as well as the most significant storage of water in the Kor river course. In addition of eroded material, a load of agricultural outputs from upstream are moved to the lake every year. Main physiographic, physical and chemical, and biological components of the lake were monthly sampled and analysed from Novembr 2010 to October 2011. Mean annual measures were water temperature 16.7 ˚C (SD=5.8), pH 8.2 (SD=0.3), dissolved oxygen 7.6 mg/l (SD=1.7), ammonium 0.05 mg/l (SD=0.08), nitrite 0.004 mg/l (SD=0.003), nitrate 0.44 mg/l (SD=0.44), phosphate 0.20 mg/l (SD=0.23), silicate 7.00 mg/l (SD=2.49), BOD 2.29 mg/l (SD=1.00), COD 24.49 mg/l (SD=15.47), electrical conductivity 720 µs/cm (SD=219), total dissolved solids 457.67 mg/l (SD=138.72), calcium hardness 61.30 mg/l (SD=13.45), and magnesium hardness 20.23 mg/l (SD=9.18). Living parts of the ecosystem including species of fish, orders and families of benthic invertebrates, zooplanktons, and phytoplanktons were investigated. Benthic macroinvertebrates included various nematodes, oligochates, gastropods, bivalves, and insect larvae (dipteral and coleptera), most abundant of which were dipteran larvae, mollusks, and nematodes. Dominant zooplankton was Bosmina, with Daphnia and Cyclops in next ranks. Among rotifer, Trichocera was more abundant than Lecanae. Bosmina showed the most abundance in summer and Daphnia in spring. Trichocera had its most abundance in summer as well, while Lecanae was more abundant in Automn and winter. A total of 41 algal genera from 8 divisions were found. Dominant phytoplankton division was Chlorophyta, with genus Pediastrum. Dominant microalga was Dynobrion (Chrysophyta) in Spring, Cylindrospermum and (both Cyanophyta) in Summer, Microsystis in Automn, and Ceratium (Dynophyta) in Winter. Ceratium were found in all seasons in most of the sampling sites, and showed highest abundance compared to other genera of its division. Dominant genus of diatoms was Fragilaria. Fish populations include Alburnus mossulensis, Capoeta aculeate, Capoeta damascina, Carasobarbus luteus, Carassius gibelio, Cyprinus carpio, and Hypophthalmichthys molitrix. Main different parts of the lake showed quite different composition of fish species. An epidemic occurance of the crustacean ectoparasite Lernea cyprinacea among most fishes was another important finding. These findings will serve as a base for future studies as a set point of drought situations.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Limnology ; Reservoir ; Plankton ; Fish ; Alburnus mossulensis ; Capoeta aculeate ; Capoeta damascina ; Carasobarbus luteus ; Carassius gibelio ; Cyprinus carpio ; Hypophthalmichthys molitrix
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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  • 5
    Publication Date: 2021-05-19
    Description: Abbarik River is an important river in Kerman. Study of limnology of Abbarik River for aquatics & culture was started from Mehr 1381 to Day 1382. Base of this study was physical- chemical and Biological factors. In this research 3 stations was appointed. PH- oxygen- salinity- Ca- Mg- Na- K- Cl and was majored and bentetic- fish was recognized. This study was achievement in 4 season and twice in month. Bentosis recognize was Ephemeroptera- Diptera- Anisoptera- Cleoptera Trichoptera Fishes recognize was Capoeta damascina- Nemachilus sargadensis- Cyprinion watsoni.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physical ; Chemical ; Biological ; Limnology ; Bentosis fish ; Aquatic ; Culture ; PH ; Salinity ; Oxygen ; Ephemeroptera ; Diptera ; Anisoptera ; Cleoptera Trichoptera ; Capoeta damascina ; Nemachilus sargadensis ; Cyprinion watsoni
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 44pp.
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  • 6
    Publication Date: 2021-05-19
    Description: The shiroud river is one of the important riveres in point of view of fisheries matter which located in western part of Mazndran provience. The project of Limnological study have done in this river during June/ 2002 - Jun / 2003 for more and better underestanding situations, of biological and nonbiological parameters like as phytoplankton, zooplonkton, benthoses, Ichthyology, physico - chemical and pesticides of water. - Physico- chemical paramaters by ASTM methods and spectrophotometer and pestisides by US-EPA- method and GC- ECD determined. The procedures of detemination of zooplanklon and phytoplankton by manuals of Newell, 1970 and Sorina, 1974 idantify and determined respectinely. In Ichthyology, by manual procedure Berg, 1949 identifind. The sampeling have been in four stations during four seasons and result as follow: - The maximum and minimum amount of pH were in station 5 (spring) and Station no. 4 (summer) 8.19 and 7.75 respectively. - The maximum and minimum temperature of water were in station no. 8 (summer) and stations no. 2 (winter) 27.7 c and 7.7c respectively. The fluctuatcions of nutroients datas of this study and other studies done during last 5 years showed, there were not big diffrence between them but also these datas were very close to each other. - The maximum residues of pesticides for heptachlor Epoxide (Winter ) and Lindane (spring) betweenresidues of sixteen pesticdes were 79.4 and 3.7 ppb respectively. - The maximum and minimum amount of phytoplankton biomass belong to chrysophyta during four seasons were 2572 through 1081 mg/m3. - The percentage of zooplankton belong to Rotatoria , Protozoa , Copepoda , Cladocera and Zoobenthos were in 41 , 31 , 12 , 4 and 12 percent recpectively. - The maximum aboundant percentage of bentheic animals belong to Diptera with 42 percent and were dominate whole sampeling stations . - The abundence of fishes in this river belong to two species of C. capoeta gracilis and Al. bipunctatus were catched in four seasons at eight stations . The percentages of C. capoeta gracilis species were catched in four seasons at eight stations during winter, summer , spring and fall with 96 ,81 , 73 and 67 percent respectively. The percentages of Al. bipunctatus species were catched in four seasons at eight stations during winter, summer , spring and fall with 47 , 44 , 43 and 40 percent respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Pesticdes residues ; Phytoplankton ; Zooplankton ; Riverine fishes ; Nutrients ; Limnology ; ASTM ; Rotatoria ; Protozoa ; Copepoda ; Cladocera ; Zoobenthos
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 127pp.
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  • 7
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    Woods Hole Oceanographic Institution
    Publication Date: 2022-05-26
    Description: This is a report describing activities associated with the Matamek program in 1977. Research was conducted on biological, chemical and physical factors related to salmonid production in Matamek River and Matamek Lake. Canadian universities, the Quebec government and Woods Hole Oceanographic Institution cooperated in the program.
    Description: Supported by Woods Hole Oceanographic Institution and the Department of Tourism, Fish and Game of the Province of Quebec.
    Keywords: Ecology ; Limnology
    Repository Name: Woods Hole Open Access Server
    Type: Technical Report
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  • 8
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    Universidade Estadual de Maringá. Departamento de Biologia. Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais.
    Publication Date: 2022-05-13
    Description: The environmental variables are important in the functioning and dynamics of freshwater ecosystems, since changes in physical and chemical conditions of these environments contribute to alterations in the ecological functioning. This research was conducted in four neotropical flood plains: Amazônica, Araguaia, Pantanal e Paraná. The samples of zooplankton were performed in the subsurface region of limnetic lakes each floodplain, in the years 2011 and 2012 (dry and wet). At the same time the environmental variables were measured and obtained water samples for later laboratory analysis. Our goal was to investigate the effect of environmental variables in the abundance of copepods, and the following hypothesis was tested: HI) the abundance of copepods has a great set with environmental variables, and extremes range of environmental variation, the lower the abundance of these organisms, unimodal effect. Local factors (physical and chemical environmental conditions) determined the abundance of copepods, as well as the environmental gradient showed a close relationship in the spatial and temporal distribution of the species, being possible to observe higher abundance at specific levels of environmental variables (toward the great). Studies that focus on tolerance limits of the species and their relationships with environmental conditions has risen in recent years, as the anthropogenic changes in climate and natural environments has caused significant changes in community structure, facilitating the invasion by non-native species and loss species diversity and the consequent change in ecosystem functioning. However, the hypothesis (HI) was partially supported, as the abundance follows a pattern of rise towards the great, but it was not possible to clearly unimodal effect on abundance for many species.
    Description: As variáveis ambientais têm papel relevante no funcionamento e dinâmica de ambientes aquáticos continentais, uma vez que, mudanças nas condições físicas e químicas desses ambientes contribuem para alterações no padrão de funcionamento ecológico. Esta investigação foi realizada em quatro planícies de inundação neotropicais: Amazônica, Araguaia, Pantanal e Paraná. As coletas de organismos zooplanctônicos foram realizadas na subsuperfície da região limnética das lagoas de cada planície de inundação, nos anos de 2011 e 2012 (seca e cheia). Ao mesmo tempo foram medidas as variáveis ambientais e obtidas amostras de água para posterior análise em laboratório. O nosso objetivo foi investigar o efeito das variáveis ambientais na abundância dos copépodes, e foi testada a seguinte hipótese: HI) a abundância de copépodes possui um ótimo estabelecido com as variáveis ambientais, e em extremos de amplitude de variação ambiental, menor será a abundância desses organismos, efeito uninodal. Os fatores locais (condições físicas e químicas do ambiente) determinaram a abundância de copépodes, bem como a variação ambiental apresentou uma estreita relação na distribuição espacial e temporal das espécies, sendo possível observar maiores valores de abundância em níveis específicos das variáveis ambientais (direção ao ótimo). Estudos que focam nos limites de tolerância das espécies e suas relações com as condições ambientais tem ressurgido nos últimos anos, pois as alterações antropogênicas no clima e nos ambientes naturais tem causado expressivas mudanças na estrutura da comunidade, facilitação à invasão por espécies não nativas e perda da diversidade de espécies e a consequente alteração no funcionamento ecossistêmico. Contudo, a hipótese (HI) foi parcialmente corroborada, pois a abundância segue um padrão de ascensão em direção ao ótimo, porém não foi possível estabelecer claramente para muitas espécies um efeito unimodal na distribuição da abundância.
    Description: Masters
    Keywords: Copepoda ; Ciências Ambientais ; Ecology ; Community ; Abundance ; Spatial distribution ; Niche ; Brazil ; Temporal distribution ; Abundância ; Limnologia ; Distribuição espacial ; Nicho ; Copépodes (Crustacea: Copepoda) ; Planícies de inundação ; Comunidades, Ecologia de ; Brasil ; Distribuição temporal ; Limnology
    Repository Name: AquaDocs
    Type: Thesis/Dissertation
    Format: 37pp.
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  • 9
    Publication Date: 2022-05-26
    Description: © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Geophysical Research Letters 44 (2017): 209–218, doi:10.1002/2016GL071378.
    Description: Global lake volume estimates are scarce, highly variable, and poorly documented. We developed a rigorous method for estimating global lake depth and volume based on the Hurst coefficient of Earth's surface, which provides a mechanistic connection between lake area and volume. Volume-area scaling based on the Hurst coefficient is accurate and consistent when applied to lake data sets spanning diverse regions. We applied these relationships to a global lake area census to estimate global lake volume and depth. The volume of Earth's lakes is 199,000 km3 (95% confidence interval 196,000–202,000 km3). This volume is in the range of historical estimates (166,000–280,000 km3), but the overall mean depth of 41.8 m (95% CI 41.2–42.4 m) is significantly lower than previous estimates (62–151 m). These results highlight and constrain the relative scarcity of lake waters in the hydrosphere and have implications for the role of lakes in global biogeochemical cycles.
    Description: Knut and Alice Wallenberg Foundation; National Science Foundation Graduate Research Fellowship Program Grant Number: 2388357; National Science Foundation Grant Number: OCE-1315201
    Keywords: Limnology ; Topograhy ; Scaling ; Volume ; Mean depth
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 10
    Publication Date: 2021-05-19
    Description: This work includes a study of the ecological characteristics and of the distribution of floristic and faunistic communities in a muddy lagoon with large fluctuations of salinity.
    Description: Ce travail est une étude des conditions écologiques générales et de la répartition des peuplements végétaux et animaux dans une lagune envasée soumise à de grandes fluctuations de salinité.
    Description: Published
    Keywords: Salinity ; Lakes ; Climate ; Limnology ; Water analysis ; Sediment analysis ; Freshwater ecology ; Brackish ; Freshwater
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp 115-124
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  • 11
    Keywords: Aquatic ecosystem monitoring ; Aquatic ecosystem assessment ; Aquatic ecosystem management ; Aquatic ecosystem services ; Aquatic ecosystem policy ; Restoration ; Conservation ; Biological indicators ; Streams/ Rivers/ Lakes/ Wetlands ; Aquatic ecology ; Ecohydrology ; Limnology
    Description / Table of Contents: Today, sustainability of a healthy freshwater ecosystem and its associated ecosystem services are hot issues with ever-growing attention placed upon them. We are increasingly recognizing that they are crucial for the survival of the aquatic biota and human beings on our planet. The efficient monitoring of water resources is fundamental for effective management of water quality and aquatic ecosystems. The first stage in sustainable ecosystem management is the evaluation of the current status of target ecosystems. Traditionally, and even today, physico-chemical parameters have mainly been used to evaluate the quality of water resources. However, they have a large limit to grab the wholeness of water system, particularly in the sense of ecosystem health and integrity, for which ecological monitoring should be based on biological factors. Various approaches are applicable to ecosystem health assessment at different levels of the biological hierarchy, from genes to ecosystems. This Special Issue is designed to improve scientific understanding and strategies for sound aquatic ecosystem management and services for researchers, decision makers, and stakeholders.
    Pages: Online-Ressource (XVI, 422 Seiten)
    Edition: Printed Edition of the Special Issue Published in Water
    ISBN: 9783038422679
    Language: English
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  • 12
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    Ministry of Environment and Natural Resources, LVEMP Water Quality Component | Kisumu, Kenya
    Publication Date: 2021-05-19
    Description: Sampling for nutrients, Chrolophyll-a and total suspended solids was done in 9 in-lake stations in the Kenyan part of Lake Victoria between December 2000 and October 2001. In-situ measurements of temperature, dissolved oxygen and water transparency were also carried out. PO4-P was found to range from 0.007mg/l to 0.057 mg/l and was higher in the pelagic stations than in littoral stations. NO3-N concentrations of 0.005-0.037 mg/l were recorded and were found to be relatively higher in the littoral stations than in the pelagic stations. TN:TP ration of the lake was found to be 6.78 indicating a possibility of heterocystous blue-green algae dominating. The ratio of 8.6 in the littoral stations showed a potential for higher photosynthetic rates in this part of the lake than in the pelagic zones. SRSi levels in the pelagic zones were found to have reduced significantly compared to those reported by Talling (1965), whereas those in the littoral zone was within the reported range. Water temperature was found to have increased and transparency values decreased compared to those measured by Worthington (1930) for both the open waters and within the gulf. Anoxic conditions (DO 〈1mg/l) were measured in depths of up to 30m.
    Description: Published
    Keywords: Water quality ; Limnology ; Nutrients (mineral)
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 11pp.
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  • 13
    Publication Date: 2021-05-19
    Description: The objectives of the Lake Victoria environment monitoring were to obtain data for: (1) Determination of the present state of the lake water quality and ecosystem. (2) Analysis of the relative importance of the biological processes and limiting factors in the eutrophication of the lake. (3) Calibration of the Lake Victoria Water Quality Framework Model. (4) Long -term monitoring of changes taking place in the lake. To achieve these objectives a programme for monthly lake monitoring on the lake was established to gather data on the quality of the waters of the lake and the lake’s limnology through in-situ measurements, and laboratory analysis of samples.
    Description: Published
    Keywords: Water quality ; Urbanization ; Eutrophication ; Limnology
    Repository Name: AquaDocs
    Type: Book Section , Not Known
    Format: pp.164- 212 [Chapter 8]
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  • 14
    Publication Date: 2021-05-19
    Description: This paper reports on the major limnological and water quality findings in Lake Victoria, Kenya and offers suggestions on sustainable management of lake water quality.
    Description: Published
    Keywords: Water quality ; Eutrophication ; Resource management ; Limnology
    Repository Name: AquaDocs
    Type: Book Section , Not Known
    Format: pp.239-261 [Chapter 10]
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  • 15
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 199 (1989), S. 165-174 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During ontogeny, the apical and basal components of dicamptodontid teeth exhibit three major developmental stages: nonpedicellate, subpedicellate, and pedicellate. Premetamorphic larvae tend to have nonpedicellate teeth, incompletely or recently metamorphosed individuals tend to have subpedicellate teeth, and fully transformed adults usually have pedicellate teeth. In concert with this transition, cusp morphology is modified from a larval monocuspid, to an incipiently bicuspid, to definitive adult bicuspid, and finally to an adult monocuspid condition. Thus, the larval and adult monocuspid conditions are ontogenetically distinct. The morphology of the larval monocuspid, adult bicuspid, and adult monocuspid conditions differs between Dicamptodon and Rhyacotriton. However, the incipient bicuspid condition in these two genera is very similar in appearance, suggesting that Dicamptodon and Rhyacotriton may be more closely related to each other than to the family Ambystomatidae in which they both sometimes are placed. The method of establishing ontogenetic trajectories seems to be preferable to comparisons based on adult structure, since similarities in the morphology of adults often is owing to convergent or parallel evolution.
    Additional Material: 5 Ill.
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  • 16
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 199 (1989), S. 207-221 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Comparative morphological analysis of the female reproductive tract in macroglossine bats was undertaken to test the hypothesis that nectarivory arose at least twice within Old World fruit bats. Given that features of the female reproductive tract are not directly involved in adaptations for feeding, this data set should provide a test of the monophyly of macroglossine bats. A cladistic analysis of variation in the structure of the ovaries, oviducts, uterus, and external genitalia supports the hypothesis that Megaloglossus has developed a nectar-feeding habit independent of other macroglossine genera. Most of the variation in female reproductive organs among pteropodids is found in the development of derived external and internal features of the uterus. Fusion of uterine cornua, expansion of the common uterine body, and elaboration of the cervical region are found in a group which includes species of Pteropus, Dobsonia, Nyctimene, and the macroglossines (excluding Megaloglossus). Results of this study are concordant with independent data sets, thus providing a phylogenetic framework to evaluate critically structural and functional design in the evolution of pteropodid feeding mechanisms.
    Additional Material: 8 Ill.
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  • 17
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 200 (1989), S. 269-300 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Descriptive data are provided for ontogeny of bone to metamorphosis in the myobatrachine species Uperoleia trachyderma; in pre- and postmetamorphic specimens of U. lithomoda, Crinia signifera, and Pseudophryne bibroni; and in postmetamorphic specimens of U. laevigata. Data derived from postmetamorphic U. laevigata indicate that dermal and endochondral elements ossify independently of each other in Uperoleia. Crinia signifera does not show the same degree of independence of ossification of dermal and endochondral elements as Uperoleia, whereas dermal and endochondral elements are not independent in P. bibroni. Ten (or possibly eleven) features are identified as being influenced by heterochrony within Uperoleia, confirming that the genus represents a highly pedomorphic lineage, four elements are influenced by heterochrony in Crinia, but only two in Pseudophryne.
    Additional Material: 15 Ill.
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  • 18
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 200 (1989), S. 301-319 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Investigation of eight developmental stages by means of serial sections and subsequent graphic or wax model reconstructions, as well as by means of cleared-and-stained and dissected material, revealed that the ethmoidal endocranium in Pipa pipa consists in early states of a single horizontal ethmoid plate lacking labial cartilages. Later in the course of development, structures comparable with those in other anurans appear, though modified and of reduced size. These adult structures arise from the new cartilaginous tissue located above the former larval ethmoid plate, whereas the latter entirely disappear. This phenomenon can be observed also in P. carvalhoi and in Xenopus laevis; hence, it supposedly occurs in all pipids. On the other hand, in anuran larvae, which develop cornua trabecularum in the ethmoidal region, these persist in adults as part of the nasal septum. Positional and developmental differences suggest that, although the ethmoid plate and the cornua trabecularum arise from the same region of the cranial neural crest, they are not fully corresponding structures. Comparison with adults of other pipid genera confirmed the conclusion of some earlier investigators that P. pipa is the most specialized among pipids.
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  • 19
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The Harderian gland of the musk shrew Suncus murinus is elongated anteroposteriorly from in front of the eye to behind the ear. The gland is divided into two portions: an anterior portion (A portion) and a posterior portion (P portion). The single secretory duct of the gland emerges from the anterior end of the P portion, receives several secretory ducts of the A portion during the course along it, runs around the ventral aspect of the eyeball, and finally opens into the anterior corner of conjunctival sacs. The two portions of the gland show a fundamentally similar histological structure, having a poorly developed intraglandular duct system and wide tubular alveoli. The quantity of lipid vacuoles and stromal connective tissue in the A portion is greater than in the P portion. The lipid vacuoles in both portions are surrounded by unit membranes, but their contents appear different.The lacrimal gland of the musk shrew is located along the ventral side of the P portion of the Harderian gland. The lacrimal duct emerges from its anterior end, runs around the ventral and anterior aspects of the ear, crosses the A portion of the Harderian gapos; and, and finally opens at the posterior corner of conjunctival sacs. The lobules of the lacrimal gland comprise a branched duct system and terminal acini with two types of secretory cells: (1) acidic cells positive both for the periodic acid-Schiff reaction (PAS) and for Alcian blue (AB) and (2) neutral cells positive for PAS and negative for AB. Both cell types tend to make separate acini, but when present in the same acinus, the acidic cells occupy relatively peripheral positions in the acinus. Both cell types lack intercellular canaliculi.On the basis of the present study as well as previous descriptions in the literature, the author suggests that the mammalian lacrimal glands can be divided into two sets: (1) a Glandula lacrimalis superior with multiple secretory ducts associated with the upper eyelid and (2) a Glandula lacrimalis inferior with a single secretory duct opening into the lateral corner of the conjunctival sacs. These glands have a fundamentally similar histological structure; but in the rabbit, which possesses both sets of lacrimal glands, they are different. On the other hand, the secretory cells of lacrimal glands generally have no intercellular secretory canaliculi, which are characteristically present between the serous secretory cells of the salivary glands.
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  • 20
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The leg musculature from 11, 14, and 17 day chick embryos was analyzed histochemically to investigate the temporal and spatial distribution of various types of sulfated glycosaminoglycans present during skeletal muscle development. Types of glycans were identified by selective degradation with specific glycosidases and nitrous acid coupled with Alcian blue staining procedures for sulfated polyanions and with [35S]sulfate autoradiography. On day 11, radiolabeled chondroitin sulfate glycosaminoglycans are localized extracellularly in both the myogenic and connective tissue cell populations. By day 17, incorporation of [35S]sulfate into chondroitin sulfate is substantially reduced, although Alcian blue-stained chondroitin sulfate molecules are still detectable. With increasing age and developmental state of the tissues, radiolabeled and stained dermatan sulfate and heparan sulfate progressively increase in relative quantity compared to chondroitin sulfate both in muscle and in associated connective tissue elements. These changes in glycosaminoglycans correlate well with similar changes previously determined biochemically and further document the alterations in extracellular matrix components during embryonic skeletal myogenesis.
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  • 21
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    Journal of Morphology 201 (1989), S. 119-129 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The aquiferous system of representatives of the orders Dictyocer-atida, Dendroceratida, and Verongida has been studied to note its relevance to the systematics of the groups. The volume of the choanocyte chamber, the size and shape of the choanocytes, the number of choanocytes per chamber, the relative development of the mesohyl, and the features of endopinacocytes are estimated from scanning and transmission electron microscopic observations of representatives of most families of the three orders. Although the Dysideidae have a reticulate skeleton and were classified in the order Dictyoceratida, they are actually closer to the Aplysillidae (Dendroceratida) than to dictyoceratids. The anatomy and cytology of the Halisarcidae differ profoundly from those of these three orders and are clearly more closely related to nonkeratose sponges. Some changes in classification lead to a pattern with highly homogeneous orders that clearly differ in their anatomic and cytologic features, which does not support the hypothesis of a common origin of the “keratose” sponges.
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  • 22
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    Journal of Morphology 201 (1989), S. 161-178 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The wall of the asymmetrical saclike lungs of the fishes Polypterus and Erpetoichthys consists of several functionally different tissue layers. Their lumen is lined by a surface epithelium composed of (1) highly attenuated cells, termed pneumocytes I; (2) pneumocytes II with lamellar bodies, presumably indicating surfactant production; (3) mucous cells; and (4) ciliated cells. Underlying the pneumocytes I is a dense capillary net. The thin continuous endothelium of this net, together with the pneumocytes I, constitute the very thin blood-air barrier. The basement membrane of epithelium and endothelium fuse in the area of the blood-air barrier (thickness 210 m̈m). Secretory and ciliary cells form longitudinal rows in the epithelium. Below the zone with a gas-exchanging tissue, a layer of connective tissue containing collagen and special elastic fibers occurs. The blood vessels that give rise to or drain the superficial capillary plexus are located in this connective tissue. The outermost layer of the lung consists of muscle cells, a narrow inner zone with smooth muscle cells, and an outer, broader zone with cross-striated muscle cells. The lung is innervated by myelinated and nonmyelinated nerve fibers. The morphology of the gas-exchange tissue in the lungs of these primitive bony fish is fundamentally very similar to that of the lungs of tetrapod vertebrates. The morphologic observations are in close agreement with physiologic data, disclosing well-developed respiratory capacities. Structural simplicity can be regarded as a model from which the lungs of the higher vertebrates derived. In addition to respiratory function, the lungs seem also to have hydrostatic tasks.
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  • 23
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    Journal of Morphology 201 (1989), S. 179-186 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Effect of turning of the egg during incubation on development of the area vasculosa of the chick embryo was investigated. The size of the area vasculosa was determined by two methods: direct measurement with calipers and measurement of a template cut from the eggshell by use of an automatic surface area recorder. The effects of turning and additionally the effects of lowered temperature (36°C) on both growth of the area vasculosa by day 7 and embryo growth by day 14 of incubation were investigated. The effects of turning during a critical period for turning, from 3 to 7 days of incubation, were also recorded. Generally, failure to turn eggs retarded growth of the area vasculosa. Turning during the critical period stimulated the extent of growth of the area vasculosa by day 7 of incubation and of subsequent embryonic growth by day 14. Incubation at low temperature resulted both in reduced expansion of the area vasculosa and retarded embryonic growth in a pattern similar to that observed for unturned eggs. It is suggested that turning stimulates development of blood vessels in the area vasculosa via localized increases in blood pressure. The effect of a reduced area vasculosa is considered to retard embryonic development through restricted nutrient uptake from the yolk. The prevailing hypothesis that turning prevents deleterious membrane adhesions is questioned in light of these observations. It is suggested that the physiological basis for the need for turning lies in maximizing the growth rate of the area vasculosa to maximize yolk use and embryonic growth rate.
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  • 24
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    Journal of Morphology 200 (1989), S. 123-130 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Recessive mutant gene c in axolotls results in a failure of the heart to function because of abnormal embryonic induction processes. The myocardium in this mutant lacks organized sarcomeric myofibrils. The present study was undertaken to determine if developmental abnormalities were evident in other areas of the heart besides the myocardium. A detailed comparative survey of the structure of developing normal and mutant hearts, including the endocardium, its cellular derivatives, and the extracellular matrix, known as cardiac jelly, showed that in the mutant there are fewer than the normal number of endocardial cells lining the heart lumen, the number of mesenchyme cells is reduced, and the cardiac jelly area is greatly enlarged in the posterior part of the truncus adjacent to the ventricle.
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  • 25
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    Journal of Morphology 200 (1989), S. 163-174 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Plethodontid salamanders have unique nasolabial grooves that may function as “capillary tubes” to convey chemicals to the vomeronasal organ when these animals nose-tap. 3H-proline was placed at the base of these grooves in Plethodon cinereus, and autoradiography revealed large concentrations of radioactive material in the vomeronasal organs. There was no significant accumulation of radioactive material in the main olfactory epithelium. Salamanders with blocked nasolabial grooves lacked significant accumulation of material in their nasolabial grooves or vomeronasal epithelia, although some salamanders had radioactive material in the posterior portion of their vomeronasal organ that had entered through the internal nares. Anteriorly placed vomeronasal organs situated adjacent to the posterior limits of the nasolabial grooves may insure that nose-tapping primarily stimulates the vomeronasal sensory epithelium.
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  • 26
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    Journal of Morphology 200 (1989) 
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  • 27
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    Journal of Morphology 200 (1989), S. 231-245 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A new mechanical model for function of the pharyngeal jaw apparatus in generalized perciform fishes is developed from work with the family Haemulidae. The model is based on anatomical observations, patterns of muscle activity during feeding (electromyography), and the actions of directly stimulated muscles. The primary working stroke of the pharyngeal apparatus involves simultaneous upper jaw depression and retraction against a stabilized and elevating lower jaw. The working stroke is characterized by overlapping activity in most branchial muscles and is resolved into three phases. Four muscles (obliquus dorsalis 3, levator posterior, levator externus 3/4, and obliquus posterior) that act to depress the upper jaws become active in the first phase. Next, the retractor dorsalis, the only upper jaw retracting muscle, becomes active. Finally, there is activity in several muscles (transversus ventrales, pharyngocleithralis externus, pharyngohyoideus, and protractor pectoralis) that attach to the lower jaws. The combined effect of these muscles is to elevate and stabilize the lower jaws against the depressing and retracting upper jaws.The model identifies a novel mechanism of upper jaw depression, here proposed to be the primary component of the perciform pharyngeal jaw bite. The key to this mechanism is the joint between the epibranchial and toothed pharyngobranchial of arches 3 and 4. Dorsal rotation of epibranchials 3 and 4 about the insertion of the obliquus posterior depresses the lateral border of pharyngobranchials 3 and 4 (upper jaw). The obliquus dorsalis 3 muscle crosses the epibranchial-pharyngo-branchial joint in arches 3 and 4, and several additional muscles effect epibranchial rotation. Five upper jaw muscles cause upper jaw depression upon electrical stimulation: the obliquus dorsalis 3, levator posterior, levator externus 3/4, obliquus posterior, and transversus dorsalis. This result directly contradicts previous interpretations of function for the first three muscles. The presence of strong depression of the upper pharyngeal jaws explains the ability of many generalized perciform fishes to crush hard prey in their pharyngeal apparatus.
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  • 28
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    Journal of Morphology 200 (1989), S. 255-267 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Changes in body morphology during growth and reproduction in the hydromedusa Eleutheria dichotoma are described in terms of variations in eight different characters: umbrella diameter, total surface area, tentacle area, umbrella area, tentacle knob diameter, number of embryos, and diameter and area of buds. Sexually (sex) and vegetatively (veg) reproducing medusae differ significantly in their body morphometrics. Statistically significant allometric relations exist between umbrella diameter and (1) central area (sex and veg); (2) tentacle area (veg); (3) total area (veg); (4) tentacle knob diameter (veg); (5) bud diameter; and (6) number of embryos. A significant correlation between umbrella diameter and area is also found in undetached buds. During sexual reproduction, umbrella area shows positive allometry and loses its correlations to total area, tentacle area, and tentacle knob diameter. Linear and nonlinear bivariate allometric coefficients allow estimation of total body size from only one or two easily measurable attributes, e.g., umbrella and tentacle knob diameter. Curve fitting by the classic allometric equation (y = bxc) is only negligibly worse than that obtained with a “full” equation (y = a + c), and statistical confidence is better.Chemical analyses for carbon and nitrogen content allow estimation of biomass from the projection area of the body surface. The relation factors are 1.06 μgC mm-2 (sex) and 1.14 μgC mm-2 (veg) for carbon and 0.293 μgN mm-2 (sex) and 0.287 μgN mm-2 (veg) for nitrogen. The C:N ratios are 3.6 and 4.0 for sexual and vegetative medusae, respectively. The use of allometric regression formulas to calculate surface areas and to relate these to carbon content provides quick estimations of body size in a microscopic animal.
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  • 29
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    Journal of Morphology 202 (1989), S. 13-28 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The neuronal organization of the accessory olfactory bulb (AOB), which receives sensory information from the vomeronasal organ, was described in a squamate reptile (Podarcis hispanica) by means of light microscopy. Using the Golgi-impregnation method, seven neuronal types could be distinguished:Periglomerular cells constitute a morphologically heterogeneous population of small neurons located between and around the glomeruli.The mitral cells are diffusely distributed in the AOB. Their cell bodies are usually located within the mitral cell layer, but some of them could be also observed in the plexiform layers. Mitral cells were classified into three subgroups on the basis of their sizes and dendritic tree morphologies. Thus, the “outer mitral cells” have the biggest cell bodies, and their distal secondary dendrites are mainly distributed rostrocaudally in the external plexiform layer. The “inner mitral cells” have large cell bodies, and their secondary dendrites are distributed dorsoventrally and are located deeper than those of the other two subgroups. The third type, the “small mitral cells,” is the smallest one among mitral cells in the AOB, and from their cell bodies, only two main dendritic trunks arise.The granule cells are composed of several categories based on their different cell body locations and dendritic tree morphologies. Thus, the “superficial granule cells” are located exclusively in the external plexiform layer and have small dendritic fields. The “middle granule cells” have fusiform cell bodies - situated in the internal plexiform layer - and present a wide dendritic projection area. Finally, the “deep granule cells” are distributed throughout the granule cell layer and include a great variety of dendritic tree morphologies.The distribution and morphological features of all neuronal types constituting the AOB of Podarcis were compared with those reported on other vertebrates. The results suggest that the lamination pattern and neuronal organization of the AOB in lizards are more similar to that of mammals than to that of the remaining vertebrates.
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  • 30
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    Journal of Morphology 202 (1989), S. 69-88 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Iridescent butterfly scales are structurally colored, relying upon the interaction of light with detailed architecture to produce their color. In some iridescent scales, the reflective elements are contained within the body of the scale and come in two basic forms, lattices that produce diffraction colors (analogous to those produced by opal), and stacks of laminae that produce thin-film interference colors (analogous to those produced by soap or oil films). Both structures are remarkably complex and precise, yet each is only part of the total edifice built by the cell that makes the scale.To understand better how a cell can produce lattices or thin-film laminae, I studied the development of iridescent scales from two lycaenid butterflies. The presence of diffraction and thin-film scales in the same family (and in some cases on the same individual) suggests that the two types must be developmentally related; yet these results yield no clear explanation as to how. The diffraction lattice appears to be shaped within the boundaries of the scale cell by means of a convoluted series of membranes in which the smooth endoplasmic reticulum plays an important part. The thin-film interference laminae appear to result from the condensation of a network of filaments and tubes secreted outside the boundaries of the cell. This paper outlines the developmental histories of both types of scale and discusses the developmental implications of the mechanisms by which they form.
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  • 31
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    Notes: Wing folding spicules, elytral binding patches, and elytral locking devices of adult male and female seed weevils, Smicronyx fulvus LeConte and S. sordidus LeConte, involved in stridulation are described. Sound is produced by both sexes of the two species when the plectrum, paired conical teeth located along the anterior margin of the dorsally elevated seventh sternite, is struck against an elongate file, the pars stridens, on the under surface of the apical portion of each elytron. A second plectrum, on the sixth tergite, is well-developed in males of both species and is used by males to produce sound before and during mating.Sex-specific and species-specific differences in the sound produced is attributed to structural variation in the pars stridens and the elytra. The pars stridens determines frequencies, while the elytra may further modify the sound. The frequency range for male S. fulvus is 1,000 cycles per second (cps) through 13,000 cps and for male S. sordidus is 2,500 cps through 13,000 cps. The frequency range for female S. fulvus is 2,000 cps through 11,500 cps and for female S. sordidus is 900 cps through 11,500 cps.
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  • 32
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    Journal of Morphology 202 (1989), S. 53-68 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study of neuromast ontogeny and lateral line canal formation in Oreochromis aureus and Cichlasoma nigrofasciatum reveals the existence of two classes of neuromasts: those that arise just before hatching (presumptive canal neuromasts, dorsal superficial neuromasts, gap neuromasts, and caudal fin neuromasts) and pairs of neuromasts that arise on each lateral line scale lateral to each canal segment at the same time as canal formation. In the anterior trunk canal segment, each presumptive canal neuromast is accompanied by a dorsoventrally oriented superficial neuromast forming an orthogonal neuromast pair. It is suggested that each of these dorsoventrally oriented superficial neuromasts is homologous to the transverse superficial neuromast row described by Münz (Zoomorphology 93:73-86, '79) in other cichlids. It is further suggested that the longitudinal lines described by Münz (Zoomorphology 93:73-86, '79) are derived from the pair of superficial neuromasts that arise during canal formation. Distinct changes in neuromast topography are documented. Neuromast formation, scale formation, and lateral line canal formation are three distinct and sequential processes. The distribution of neuromasts is correlated with myomere configuration; there is always one presumptive canal neuromast on each myomere. A single scale forms beneath each presumptive canal neuromast. Canal segment formation is initiated with the enclosure of each presumptive canal neuromast by an epithelial bridge which later ossifies. The distinction of these three processes raises questions as to the causal relationships among them.
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  • 33
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    Journal of Morphology 199 (1989), S. 299-311 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Gonadal differentiation in premetamorphic Bombina orientalis is described and staged. The pattern of events during differentiation in Bombina differs in several respects from that previously described in other anurans. The Bombina gonad initially develops on the ventral surface of the vena cava, where there is no pre-existent somatic genital ridge prior to the arrival of the germ cells. The sexually undifferentiated gonad does not have a distinct cortex and medulla; instead, medullary cells ingress from the mesonephric blastema during sexual differentiation. Formation of a testis or an ovary appears to depend on the ability (or lack of ability) of the medulla to invade the germ cell-containing cortex. In the germ line, sexual differetiation can be recognized by a premeiotic increase in oogonial cell volume.
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  • 34
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    Notes: The lungs of the New Caldeonian gecko Rhacodactylus leachianus were examined by means of gross dissection and light and electron microscopy. This tropical species, which is the largest living gecko, possesses two simple, single-chambered lungs. Right and left lungs are of similar size and shape. The lung volume (27.2 ml · 100 g-1) is similar to that of the tokay (Gekko gecko) but differs in that the gas exchange tissue is approximately homogeneously distributed, and the parenchymal units (ediculae) are very large, ∼2 mm in diameter. The parenchymal depth varies according to the location in the lung, being deepest near the middle of the lung and shallowest caudally. Scanning and transmission electron microscopy reveal an unusual distribution of ciliated cells in patches on the edicular walls as well as on the trabeculae. Secretory cell are very numerous, particularly in the bronchial epithelium, where they greatly outnumber the ciliated cells. The secretory cells form a morphological continuum characterized by small secretory droplets apically and large vacuoles basally. This continuum includes cells resembling type II pneumocytes but which are devoid of lamellar bodies. Type I pneumocytes similar to those of other reptiles cover the respiratory capillaries, where they form a thin, air-blood barrier together with the capillary endothelial cells and the fused basement laminae. The innervation, musculature, and vascular distribution in R. leachianus are also characterized. Apparent simplification of the lungs in this taxon may be related to features of its sluggish habits, whereas peculiarities of cell tissue composition may reflect demands of its mesic habitat.
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  • 35
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    Journal of Morphology 199 (1989) 
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  • 36
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    Journal of Morphology 199 (1989) 
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  • 37
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    Journal of Morphology 199 (1989), S. 41-52 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The molting cycle of Artemia is described and subdivided in stages A-D3 according to the system of Drach. Determination of the stages is done in living animals by light microscopic observation of changes in the texture of the setal matrix of the exopodites. A parallel ultrastructural investigation of the integument was carried out to control the proposed staging scheme. The duration of each stage was calculated.
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  • 38
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    Journal of Morphology 199 (1989), S. 71-92 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The extracellular matrix surrounding the sea urchin embryo (outer ECM) contains fibers and granules of various sizes which are organized in recognizable patterns as shown by ultrastructural studies, particularly stereoimaging techniques. The use of the ruthenium red method for retaining and staining the ECM, with modification of the Luft (Anatomical Record 171:347-368, 1971) method for invertebrate embryos, allows for the clarification of certain structures, particularly fiber compaction in the interzonal region, and microvillus-associated bodies.The inner ECM in the sea urchin embryo includes the basal lamina and blastocoel matrix. Stereoimages show that the fibers which are loosely distributed in the blastocoel matrix become compacted around the periphery of the blastocoel to form the basal lamina.The ruthenium red method was also used on a number of marine invertebrate embryos and larvae, representing different phyla, to facilitate comparisons between their surface coats. The similarities observed in the specimens shown suggest that ECMs are widely found on marine invertebrate eggs, embryos, and larvae, and that they resemble vertebrate ECMs and may, therefore, have similar functions.
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  • 39
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    Journal of Morphology 199 (1989), S. 151-164 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The coronate larva of the ascophoran bryozoan Watersipora arcuata has a ring of 32 large, multiciliated coronal cells that are used for swimming. Fourteen pairs of small cells are intercalated between the lateral margins of adjacent coronal cells. These intercoronal cells are arranged in a precise pattern and are polymorphic: seven pairs have multiple cilia and seven pairs are mono- or oligociliated. Three pairs of multiciliated intercoronal cells have their cilia arranged as a whorl that is recessed in a pocket formed between the adjacent coronal cells, and they are thought to be photoreceptors that sense general light intensity. Two other pairs of multiciliated cells with cohesive tufts of cilia may be chemo- or mechanoreceptors. Roles of the other intercoronal cells in this species are not evident, but it is proposed that the majority, if not all, of them are sensory. The close proximity of all the intercoronal cells to the equatorial nerve ring is compatible with this interpretation. Analyses of the literature on cleavage patterns, pigment cup ocelli, and flagellar tufts that serve as balancers in coronate larvae lead us to propose that (1) an intercoronal cell is the sensory element of most, if not all, pigment cup ocelli of bryozoan larvae; and (2) intercoronal cells are not modified coronal cells but probably are specialized supra- and/or infracoronal ones that have migrated to an intercoronal position.
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  • 40
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    Journal of Morphology 199 (1989), S. 245-247 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 41
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    Journal of Morphology 199 (1989), S. 223-243 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The projectile tongue of caudate amphibians has been studied from many perspectives, yet a quantitative kinetic model of tongue function has not yet been presented for generalized (nonplethodontid) terrestrial salamanders. The purposes of this paper are to describe quantitatively the kinnematics of the feeding mechanism and to present a kinetic model for the function of the tongue in the ambystomatid salamander Ambystoma tigrinum. Six kinematic variables were quantified from high-speed films of adult A. tigrinum feeding on land and in the water. Tongue protrusion reaches its maximum during peak gape, while peak tongue height is reached earlier, 15 ms after the mouth starts to open. Tongue kinematics change considerably during feeding in the water, and the tongue is not protruded past the plane of the gape. Electrical stimulation of the major tongue muscles showed that tongue projection in A. tigrinum is the combined result of activity in four muscles: the geniohyoideus, Subarcualis rectus 1, intermandibularis posterior, and interhyoideus. Stimulation of the Subarcualis rectus 1 alone does not cause tongue projection. The kinetic model produced from the kinematic and stimulation data involves both a dorsal vector (the resultant of the Subarcualis rectus 1, intermandibularis posterior, and interhyoideus) and a ventral vector (the geniohyoideus muscle), which sum to produce a resultant anterior vector that directs tongue motion out of the mouth and toward the prey. This model generates numerous testable predictions about tongue function and provides a mechanistic basis for the hypothesis that tongue projection in salamanders evolved from primitive intraoral manipulative action of the hyobranchial apparatus.
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  • 42
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    Notes: We have attempted to develop an objective, semiquantitative classification of fiber types in turtle neck and limb muscle using microphotometry and multivariate statistical techniques. We first stained serial sections for myosin adenosine triphosphatase (ATPase) (with acid and alkaline preincubation and without preincubation), NADH-diaphorase, and two glycolysis-associated markers, α-glycerophosphate dehydrogenase (α-GPDH) and glycogen phosphorylase A (GPA). This allowed us to characterize individual muscle fibers in terms of their contraction speed and metabolic properties. Next we used microphotometry to measure the optical density of the reaction product in each fiber, and we subjected the resulting optical density matrix to cluster and discriminant function analyses in order to assign fibers to groups (fiber types) and to determine which stains contribute most to the distinction between groups. As a control, we processed a well characterized mammalian muscle (rat sternomastoid) simultaneously. Our results suggest that both neck and limb muscle in Pseudemys can best described as falling into three groups: (1) slow oxidative (SO) fibers; (2) fast oxidative glycolytic (FOG) fibers, with relatively high oxidative and glycolytic capacities; and (3) fast glycolytic (Fg) fibers, with low oxidative, low/intermediate α-GPDH, and high GPA activities. These three fiber types differ from like-named types in rat muscle both in the pH lability of their myosins and in their metabolic profiles.
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  • 43
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    Journal of Morphology 200 (1989) 
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  • 44
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    Journal of Morphology 200 (1989), S. 1-8 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Distribution and density of the chloride cells in the newly hatched larvae of teleosts vary depending on species and environmental salinity at hatching. In the euryhaline freshwater ayu (Plecoglossus altivelis), chloride cells are concentrated in the skin posterior to the pectoral fins and gradually decrease in number toward the head and tail. In the stenohaline sea water flounder (Kareius bicoloratus), most chloride cells are localized at the inner membrane of gill chambers and in the skin near the openings of gill chambers, but only a few cells appear in the skin of the yolk sac. In the stenohaline freshwater carp (Cyprinus carpio), only a few small chloride cells are scattered in the body skin. The density and abundance of chloride cells appears to be correlated with the different requirements for osmoregulation in teleost larvae.
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  • 45
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    Notes: This investigation relates the occlusal morphology of the continuously growing molars of common wombats (Vombatus ursinus) to the underlying enamel ultrastructure that was investigated using the techniques of light microscopy, scanning electron microscopy, and transmission electron microscopy. The main feature of the occlusal enamel was a prominent ridge, which followed the contour of the dentine-enamel junction (DEJ). It was found that the occlusal morphology depended upon the orientation of the dentinal and enamel tissues, variations in prism orientation, Hunter-Schreger bands (HSB), and presence or absence of cleavage. Cleavage of enamel promoted by sheets of parallel prisms occurred along the face between the DEJ and the ridge, whereas on the face between the ridge and the cementum-enamel junction (CEJ) cleavage was inhibited by HSB. The slope of the latter face was mainly due to a decrease in wear resistance going from the ridge, where prisms were intercepted transversely, toward the CEJ, where they were intercepted obliquely. Occasionally small surface undulations were observed on the face between the ridge and the CEJ. These undulations were found to correspond to gradually decussating enamel regions. The pronounced cleavage of enamel parallel to the face between the DEJ and the ridge played an important role in conferring on the continuously growing molars a distinct property to develop and maintain a self-sharpening ridge throughout the life of the tooth.
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  • 46
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    Journal of Morphology 200 (1989), S. 215-230 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The internal anatomy of juveniles and adults of Hypochthonius rufulus selected as a model species representing the lower Oribatida was investigated histologically and compared with the published characteristics of higher oribatid internal anatomy. In this species, the cuticle is weak and flexible, consisting of epicuticle and endocuticle on the body, but including an exocuticle between the epicuticle and endocuticle of the legs. Walls of the mesenteron in the digestive tract are of uniform thickness and structure without any regional thickening, and there are no proventricular glands. The hindgut is apparently divided into five parts: colon 1 and 2, rectum 1 and 2, and anal atrium; food bolus exhibits a multilamellar structure in this section. The glandular system is less diversified than in some other oribatids. Tracheae are apparently lacking. Females possess only two relatively large eggs, filling one-half of opistosoma, and they lack ovipositors. Eggs are present in females during the whole year. Gonad buds appear first in the protonymph stage. Only one male was found among 146 adults studied. No male external organ (aedeagus or penis) is present.
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  • 47
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    Journal of Morphology 200 (1989), S. 199-213 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have investigated the structural organization of the wind-sensitive giant interneurons in the thoracic ganglia of adult American cockroaches. These seven bilaterally paired interneurons have long been thought to play a role in directing the wind-elicited escape response of the animal. Each of the giant interneurons was labeled individually by intracellular injection of cobaltic hexamine chloride. An individual giant interneuron could be reliably identified from animal to animal based on its branching pattern in thoracic ganglia. The axons of the giant interneurons are situated on each side of the nerve cord in two recognizable subgroups. Comparisons of the axonal arbors of the dorsal and ventral subgroups showed that they project into distinct but partly overlapping regions of thoracic ganglia. Three of the giant interneurons were found to have axonal arbors that cross the longitudinal midline of thoracic and abdominal ganglia. Bilateral pairs of these giant interneurons were labeled concomitantly, and many of the individual neurites from these cells appeared to be closely apposed. All these morphological characteristics are discussed in relation to the connectivity and functional significance of the giant interneurons.
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  • 48
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    Journal of Morphology 200 (1989), S. 247-253 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of the adductor muscle of the boring clam (Tridacna crocea) was investigated. The adductor was composed of opaque and translucent portions. The opaque portion contained smooth muscle cells; the translucent portion contained obliquely striated cells. Smooth muscle cells were classified, according to the statistically analyzed diameters of their thick myofilaments, into two types, S-1 and S-2. S-1 cells had thick myofilaments, 50-60 nm in diameter. S-2 cells had thick myofilaments of two sizes, about 55-65 nm and 85-100 nm in diameter, respectively. Obliquely striated muscle cells in the translucent portion were also classified into two types: O-1 cells, with thick myofilaments 30-35 nm in diameter, and O-2 cells, with myofilaments of 50-60 nm.
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  • 49
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    Journal of Morphology 201 (1989) 
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  • 50
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    Journal of Morphology 201 (1989), S. 23-37 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Sensory and motor innervation of the proboscis by branches of the maxillary and labial nerves of the worker honey bee has been investigated in specimens stained vitally by methylene blue or viewed by scanning electron microscopy. A chordotonal organ consisting of a single scolopidium is present in the maxillary palp. Flexion of the maxillary palp occurs only passively, induced by the flexion of the galea. This chordotonal organ may function as a proprioceptor for the movement of the galea. Another chordotonal organ exists in the prementum of the labium. It contains, on the average, 12 sensory cells and presumably responds to the bending of the labial palp. A nerve-net of bipolar cells arises from the sensory branches of the maxillary nerve. Free nerve endings derived from the periphery of this nerve-net expand broadly on the intersegmental membranes connected to the stipes. The right and left nerves to the dilator muscles of the salivarium exchange branches, resulting in the reciprocal innervation of each muscle.
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  • 51
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    Journal of Morphology 201 (1989), S. 59-68 
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    Notes: The tectorial structures of the inner ear of the proteid salamander Proteus anguinus were studied with transmission and scanning electron microscopy in order to analyze the ultrastructure of the otoconial membranes and otoconial masses of the maculae and the tectorial membrane of the papilla amphibiorum. Both otoconial and tectorial membranes consist of two parts: (1) a compact part and (2) a fibrillar part that joins the membrane with the sensory epithelium. Masses of otoconia occupy the lumina above these membranes.There are two types of calcium carbonate crystals in the otoconial masses within the inner ear of Proteus anguinus. The relatively small otoconial mass of the utricular macula occupies an area no greater than the diameter of the sensory epithelium, and it is composed of calcite crystals. On the other hand, the enormous otoconial masses of the saccular macula and the lagenar macula are composed of aragonite crystals. In the sacculus and lagena, globular structures 2-9 m̈m in diameter were discovered on the lower surfaces of the otoconial masses above the sensory epithelia. These globules show a progression from smooth-surfaced, small globules to large globules with spongelike, rough surfaces. It is hypothesized that these globules are precursors of the aragonite crystals and that calcite crystals develop similarly in the utriculus. The presence of globular precursors in adult animals suggests that the formation of new crystals in the otoconial membranes of the sacculus and lagena of Proteus is a continuous, ongoing process.
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  • 52
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    Journal of Morphology 201 (1989) 
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  • 53
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    Notes: When homozygous, recessive mutant gene c in Ambystoma mexicanum results in a failure of embryonic heart function. This failure is apparently due to abnormal inductive influences from the anterior endoderm resulting in an absence of normal sarcomeric myofibril formation. Biochemical and immunofluorescent studies were undertaken to evaluate the contractile proteins actin and tropomyosin in normal and mutant hearts. For the immunofluorescent studies, cardiac tissues were fixed in periodate-lysine-paraformaldehyde, frozen sectioned, and immunostained by an indirect method with monospecific polyclonal antibodies produced against highly purified chicken heart actin and tropomyosin. In normal hearts, both antiactin and antitropomyosin stained the myofibrillar I-bands intensely. In mutant hearts, intensity of staining with antiactin antibody was similar to normal, although sarcomeric patterns were not observed. Staining intensity for tropomyosin with antitropomyosin antibody was significantly reduced in mutant hearts when compared to normal. Biochemical studies were used to evaluate antibody specificity, antigenic variability, and relative protein concentrations of actin and tropomyosin in normal and mutant cardiac tissues. Tissue homogenates were electrophoresed in two dimensions, and second-dimension slab gels were either Coomassie Blue silver-stained or transblotted onto nitrocellulose and the proteins stained with antibodies. Stained gels and immunoblots of cardiac proteins reveal that the amounts of actin isoforms are identical in normal and mutant hearts. However, these methods demonstrate a significantly reduced amount of tropomyosin in mutant tissue. This confirms earlier studies suggesting reduced amounts of tropomyosin in mutant hearts based upon immunological assays. Thus, failure of normal myofibrillogenesis in gene c mutant hearts does not appear to result from a change in actin isoform composition but may be related to a deficiency in tropomyosin.
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  • 54
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    Journal of Morphology 202 (1989) 
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  • 55
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    Notes: Knee joints from adult, juvenile, hatchling, and embryonic (full term) American alligators were dissected to reveal the cruciate ligaments and the medial and lateral menisci. Two anterior cruciate (major and minor), a posterior cruciate, an intermeniscal, and a meniscofemoral ligament were identified. In addition, we found a fourth internal ligament which has not been reported previously. Menisci and ligaments from left knees were fixed in formalin and processed for routine histological observation. Those from right knees were stained in bulk by using a gold chloride method and were either frozen and sectioned at 100 m̈m on a sliding microtome or were processed for paraffin sections at 30 m̈m. The morphology of the collagenous, cartilaginous, and vascular constituents of the tissues was similar to that of the dog, cat, and human. Nerve fibers were observed in all tissues sampled. Structures resembling Golgi tendon organs and Pacinian corpuscles were identified, reinforcing the theory that neural elements within cruciate ligaments and menisci may provide afferent input that affects the function of the knee joint.
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  • 56
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    Journal of Morphology 202 (1989), S. 205-223 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Topics: Biology , Medicine
    Notes: The ultrastructural changes occurring in the adenohypophysis (AH) of the anadromous sea lamprey, Petromyzon marinus, during metamorphosis (stages one through seven) were examined. The rostral pars distalis initially contains one granulated (secretory) cell type A and one nongranulated type I cell. A second granulated cell (type B) appears during the later stages (stages six and seven) of metamorphosis. The most pronounced ultrastructural changes take place in the caudal pars distalis (CPD). Initially, most cells (80-90%) are nongranulated cells type II and some type I. Granulated type C and D cells form the remainder of the CPD. Almost all cells during stages three and four demonstrate a marked increase in synthetic activity evident by conspicuous Golgi regions, abundant rough endoplasmic reticulum (RER), and increased cell volume. Most cells are sparsely granulated. Secretory cell types C and D and, two new cell types, E and F, are present. Synthetic activity subsides by stage five. Most cells (80-90%) during stages five through seven are granulated. Type E are most prevalent with variable numbers of types C and D and few type F. Nongranulated cells now represent only 10-20% of the CPD. The increase in granulated cells occurs at the expense of type II cells that differentiate into granulated cell types. The fine structure of the pars intermedia throughout metamorphosis remains similar to that of the larva. Most cells are granulated, highly vesiculated type G cells. A few nongranulated type I cells are also present. The functional significance of the secretory cells in the AH is related to the requirement for an intact pituitary gland for the initiation and completion of metamorphosis.
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  • 57
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  • 58
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    Journal of Morphology 202 (1989), S. 255-269 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The gastrodermis of the black coral Antipathes aperta is associated with eight distinct types of cells, including two types of microbasic b-mastigophores (nematocysts), spumous and vesicular mucus cells, and ganglion cells that are essentially the same as in the epidermis. Three additional types of cells are unique to the gastrodermis, and are readily distinguished from those of the epidermis by their electron-opaque inclusions. These include lipoidal cells, zymogen digestive cells, and an unusual type of epitheliomuscular collar cell. The pharyngeal region is characterized by the presence of electron-opaque nematocysts, a scattering of zymogen cells, and a large number of collar cells. The latter are distinguished in part by the presence of dense microfibrillar processes that surround the microvilli and extend into adjacent collars. This interconnection results in the formation of an extensive pharyngeal meshwork. These collar cells are additionally distinguished by the placement of the collar and flagellum adjacent to a flared cup of cytoplasm. This portion of the cell is capable of endocytosis of relatively large unicellular prey, and apparently is capable of forming digestive vesicles as well. The pharyngeal gastrodermis grades into simple lobate septal filaments toward the base of the coelenteron, where large, granular nematocysts all but replace the smaller electron-opaque types Collar cells are found here as well, but in fewer numbers compared to the zymogen cells. Ultrastructural results are compared with those of other coelenterates and discussed in terms of food and modes of nutrition.
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  • 59
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    Notes: In Holland, bugs of the species Hebrus pusillus and H. ruficeps have one generation per year and overwinter as unmated adults. Males have two testes with two follicles + vasa efferentia each, paired vasa deferentia and seminal vesicles, an ejaculatory duct, and a protrusible phallus comprising an articulatory apparatus, phallotheca, endosoma, and paired claspers. The skeletomusculature of this system is described (it has 12 paired and four unpaired muscles) and its functions in generating and transferring sperm (summarized in Figs. 70-75) are reconstructed from study of living bugs, dissections, whole mounts, and serial sections.Males of both species produce sperm 〉2 mm long from stem spermatogonia in the germarium of each follicle. Initial definitive spermatogonia divide synchronously three times to form clones of eight, interconnected, primary spermatocytes. These enlarge up to 43-fold in males of H. pusillus and 78-fold in those of H. ruficeps, undergo meiosis, and, after adult emergence, complete their differentiation into bundles of 32 sperm which coil transversely about the periphery of each follicle at its base. These begin to enter the vasa efferentia in mid August, rupture, and release their sperm into the seminal vesicles where they are stored overwinter. Most spermatocyte and spermatid cysts remaining in the testes degenerate in fall, leaving only stem spermatogonia and a few early spermatocysts in the germaria.Males of H. pusillus begin to mate the first warm days of spring but only the most persistent succeed. A male jumps on the back of a female, induces her to lower her ovipositor, and, within 12 min (@ 18-24°C), introduces the endosoma of his phallus up its shaft and fills his seminal duct with sperm. The female draws this into her gynatrial sac at the end of copulation and transfers it into her spermatheca in about 30 min, the sperm reversing themselves within it so that their heads face towards its mouth. The male may stay on her back for up to 2 hours and may copulate again up to three times before leaving to mate with other females.Males of H. pusillus may be sexually active for months after overwintering, because spermatogonia in their germaria reactivate in spring to produce additional sperm. Those of H. ruficeps do not and males mate successfully only until their supply of overwintered sperm is exhausted.The chromosome complement of H. pusillus males is 2N = 22 + XY. The X and Y chromosomes are of unequal length, form a pseudo pair at metaphase I, and segregate to opposite poles at anaphase I - the first instance of pre-reductional segregation of sex chromosomes to be recorded in the Gerromorpha. The chromosome complement of H. ruficeps may be 2N = 24 + XO but the nature of two chromosomes was not resolved. The single X segregates to half the spermatids at anaphase II.
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  • 60
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    Journal of Morphology 202 (1989), S. 409-424 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The solum nasi of Microcebus murinus is characterized by the presence of a zona annularis, continuity between the anterior transverse lamina and the paraseptal cartilage, a continuous paraseptal cartilage, a palatine cartilage and a posterior transverse lamina. It lacks a fibula reuniens and possibly a cartilage of the nasopalatine duct as well as a palatine papillary cartilage. The morphology in M. murinus closely resembles that seen in Tupaia and Galago. This affinity results from the retention of primitive traits. However, Galago is reported to lack a zona annularis, thus displaying a specialization not shared with M. murinus. Therefore, the zona annularis provides a useful trait for distinguishing between the ontogenies of M. murinus and Galago.
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  • 61
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    Journal of Morphology 202 (1989), S. 435-455 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In each of 30 dipteran species, representing 13 acalyptrate and 7 calyptrate families, the cardia is formed from specialized cells at the junction between foregut and midgut. Foregut epithelium forms the stomodeal valve; midgut epithelium envelops the valve to form the cardia's outer wall. Cytological characteristics within these epithelia differ from region to region and from species to species. Since the cardia secretes the peritrophic membrane, cardias with diverse patterns of cellular differentiation may be expected to produce peritrophic membranes with similarly diverse properties. Close relatives often share more details of cardia structure than do distantly related taxa. Within the monophyletic Calyptratae, a common pattern of cellular differentiation includes three distinct zones of columnar midgut cells enclosing a flanged stomodeal valve. Among species in the paraphyletic Acalyptratae, midgut typically includes a single zone of tall columnar cells, while the valve may be spheroidal, cylindrical, conical, or flanged. The correlation of phylogenetic distance with divergence in cardia organization implies a strong influence of ancestry upon current structure, regardless of current diet. However, at least some of the observed diversity in cardia structure is associated with dietary divergence. Calyptrate flies with derived blood-feeding behavior display cellular differentiation that is simplified from that seen in calyptrate relatives with less specialized feeding habits. This evolutionary modification suggests that cardia organization and hence peritrophic membrane structure can adapt to dietary changes, with possible significance for the spatial organization of digestive processes and interactions with ingested microorganisms.
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  • 62
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    Journal of Morphology 202 (1989), S. 239-253 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The tentacular epidermis of the black coral Antipathes aperta is organized into three distinct regions, containing at least nine different types of cells. The outermost region is dominated by spirocytes along with two types of nematocytes, organized into discrete wart-like batteries. The two nematocyte types both contain microbasic b-mastigophore nematocysts. The outer boundary of the wart is marked by the presence of both spumous and vesicular mucus cells. The ciliation of the wart is contributed principally by the spirocytes. Warts are enveloped and separated from one another by an unusual neurosensory cell complex that extends from the tentacular surface to the mesogleal connective tissue foundation. Funnel-like, flagellated cells composing the complex connect with ganglion cells composing the dominant portion of the nerve net system. Branches of this complex also penetrate the central portion of the wart, making direct contact with the cnidae. The tentacular mid-region is composed of nematocytes and spirocytes in various stages of maturation, along with epitheliomuscular cell (EMC) somata. The EMC's narrow apically extend toward the tentacle surface, forming contacts with the cnidae. The basal end of the EMC expands to form the larger portion of the tentacular musculature. The inner region of tentacular epidermis is marked by a neuromuscular complex sheathed by extensions of mesoglea. The ganglion cells occur as a plexus deep within the tentacle and form polarized junctions with the EMC's, but neuromuscular synapses are not well enough defined for documentation. Polarized synapses lacking well-defined membrane thickenings characterize the interneuronal junctions. Granular cells lining the mesogleal surface appear to be responsible for mesogleal fibrillogenesis.
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  • 63
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    Journal of Morphology 202 (1989), S. 325-338 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The stages of differentiation of the inner ear sensory epithelia of the neotenous cave urodele, Proteus anguinus, was studied with light and electron microscopy. Comparative ultrastructural analysis among specimens of different sizes confirms that new sensory cells may be generated throughout life, particularly along the periphery of the saccular macula. The inner ear of Proteus contains at least four types of sensory cells that differ in their apical ciliary part.The lungs and air-filled buccal cavity may function as transducers of sound pressure in underwater conditions. Sound waves might be transmitted from the buccal cavity to the connected oval window. The very complex orientation of the sensory hair cells of the saccular macula and the large overlying saccular otoconial mass suggest that this macula facilitates orientation of Proteus in its underground aqueous habitat.
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  • 64
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    Journal of Morphology 202 (1989), S. 351-359 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In the Uloboridae, web reduction is accompanied by changes in opisthosomal shape, leg length, and web-monitoring tactics. These morphological changes make reduced-web spiders more cryptic and alter their leg leverage and centers of mass. When compared with the orb-weaver Uloborus glomosus, the irregular, reduced-web spider, Miagrammopes animotus, invests more mass in its prosoma and first legs. However, the latter species' elongate opisthosoma posteriorly shifts this region's center of mass, causing the relative position of its composite center of mass and the distribution of weight between its first and fourth legs to be similar to that of the orb-weaver. Like these species, the opisthosomal center of mass of the triangle-weaver, Hyptiotes cavatus, lies near its midpoint. However, the shorter first legs and rounder, heavier opisthosoma of Hyptiotes posteriorly shift its composite center of mass and distribute more of its weight onto its fourth legs. Consequently, the morphology of M. animotus can be adequately explained by its adaptiveness for web manipulation, balance, and weight distribution and the crypsis that these features confer as an ancillary advantage. In contrast, anatomical changes in H. cavatus are better explained as adaptations for web manipulation and crypsis.
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  • 65
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    Journal of Morphology 202 (1989), S. 425-433 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Eosinophilopoiesis in the musk shrew, Suncus murinus, a representative of the order Insectivora, was studied by light and electron microscopy. To examine biochemical features of cytoplasmic granules, extraction with proteolytic enzymes was carried out on ultrathin sections of bone marrow. In this species, eosinophils are produced in the same manner in both spleen and bone marrow. Developing eosinophils were distinguished as belonging to four stages, recognized by ultrastructural changes in cytoplasmic organelles as well as the eosinophilic granules during maturation. Granulogenesis began by budding of vacuoles containing flocculent material from the concave face of the Golgi apparatus, in the promyelocyte to myelocyte stage. The matrix of developing granules transformed into a finely granular structure, and the large spherical granules of mature eosinophils were homogeneous without crystalline cores. It was shown by proteolytic enzyme extraction that the proteinaceous cores of mature granules were uniformly removed; there was no evidence that they contained crystalloid inclusions. These results indicate that shrew eosinophils can be regarded as cells that retain a prototype of eosinophil granules, probably like those of ancestral mammals rather than those of higher living Mammalia.
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  • 66
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    Journal of Morphology 199 (1989), S. 363-378 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The chelonian carapace is composed of the endochondral ribs and vertebrae associated with a specialized dermis. The ribs are found in an aberrant position compared to those of all other tetrapods; they are superficial and dorsal to the limb girdles. This morphological arrangement, which constitutes the unique chelonian Bauplan, is examined from a developmental perspective. Embryos of Chelydra serpentina were studied during stages of carapace development. Tissue morphology, autoradiography, and indirect immunofluorescent localization of adhesion molecules indicate that the outgrowth of the embryonic carapace occurs as the result of an epithelial-mesenchymal interaction in the body wall. A carapacial ridge composed of mesenchyme of the dermis and overlying ectoderm is formed dorsal to the ectodermal boundary between somitic and lateral plate mesoderm. It is the anlage of the carapace margin, in which the ribs will eventually terminate. The ectoderm of the carapacial ridge is thickened into a pseudostratified columnar epithelium, which overlies a condensation in the mesenchyme of the dermis. Patterns of cell proliferation and the distribution of N-CAM and fibronectin in the carapacial ridge are consistent with patterns seen in other structures initiated by epithelial-mesenchymal interactions such as feathers and limb buds.Based on an analogy to this developmental mechanism in the development of the limb skeleton, a further analogy with the evolution of the limbs from lateral fin folds is used to form a hypothesis on the evolution of the carapace from elements of the primitive reptilian integument.
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  • 67
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    Journal of Morphology 200 (1989), S. 29-36 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Autologous tongues were transplanted onto the liver of the anuran Rana catesbeiana, specimens of which were sacrificed at intervals from 6 hr to 5 months after transplantation. Light and electron microscopy as well as histochemistry disclose that the grafts start to organize into cysts after 14 days. The taste organs occur in all grafts irrespective of the age of the graft. All surviving taste organs exhibit intense adenosine triphosphatase (ATPase) activity and contain fluorescent basal cells of the usual type. Ultrastructurally, the taste organs are composed of three distinct types of cells that lack nerves. The taste and basal cells retain the characteristic dense-core granules in their cytoplasm through the experimental periods. The present results suggested that the taste organs of Rana can survive ectopically in the liver for up to 5 months.
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  • 68
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    Journal of Morphology 200 (1989), S. 63-69 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Epithelium of the sea anemone Acontiophorum niveum is modified into four general arrangements of microappendages: (1) uniform microvilli covering pedal disc and column, (2) an interspersion of microvilli, ciliary cones, and kinocilia on tentacles, (3) flagella among an understory of microvilli from the oral disc, actinopharynx, filaments, and acontia, and (4) sparse flagella among irregular microvilli from endoderm. These arrangements are similar to those described previously in the epithelia of other anthozoans.
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  • 69
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    Journal of Morphology 200 (1989), S. 87-92 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Inserting on the buccal and esophageal foregut of Gammarus minus are numerous pairs of serially arranged dorsal dilator muscles, a single pair of lateral muscles, and two pairs of posterior muscles. Muscles of the cardiac stomach include three dorsal sets, a single pair associated with the pterocardiac ossicles, and two pairs inserting on the ventral aspect. A single pair of muscles inserts on the lateral aspect of the pyloric stomach. The extrinsic muscles of the foregut originate from exoskeletal apodemes of the cephalothoracic cuticle, sockets of the mandible, and a maxillary bridge that lies just ventral to the cardiac stomach. The extrinsic musculature of the hindgut is restricted to the rectal region and consists of paired dorsal and ventral series in an X-configuration. A single unpaired muscle inserts on the ventral midline. Extrinsic muscles of the hindgut originate from the integument of the last pleonic segment. The general arrangement of extrinsic gut muscles in G. minus is similar but not identical to that of other amphipods studied. However, the pattern is quite different from that of other malacostracans.
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  • 70
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    Journal of Morphology 199 (1989), S. 1-13 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study examines the morphology of sporadic congenital microphthalmia in 1-day-old chicks, with particular emphasis on the neural retina. On the basis of the size of the eyeball it is possible to classify microphthalmia into two groups, severe and mild. In severe microphthalmia (less than 5 mm in equatorial diameter), the eyeball is severely malformed, but in most cases it shows evidence of an organized neural retina. Although ganglion cells and an optic nerve head are present in a small proportion of these retinae, we could not trace an optic nerve projection to the brain. These results indicate that some ganglion cells are able to be sustained after the period of naturally occurring cell death, suggesting either that those ganglion cells have established some contact with the central nervous system or that the presence of their axons in a rudimentary optic nerve is adequate for survival. In mild microphthalmia (greater than 5 mm in equatorial diameter), the most consistent abnormality is a defect in the pecten, which together with other abnormalities such as orbital cysts and colobomas indicates that the major abnormality occurs in the region of the choroid fissure. Associated with these defects are abnormalities within the ganglion cell layer. In some cases the number of ganglion cells was reduced, and in others the numbers of both ganglion and displaced amacrine cells were reduced. Unexpectedly, there were localized regions completely devoid of cells in the ganglion cell layer. The timing of the congenital defect may provide some clue as to the presence of a critical period in which displaced amacrine cells are formed or are sensitive to events related to ganglion cell loss.
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  • 71
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    Journal of Morphology 199 (1989), S. 93-101 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The muscle-fiber architecture of 29 muscles from six rabbits (Oryctolagus cuniculus) was measured in order to describe the muscular properties of this cursorial animal, which possesses several specific skeletal adaptations. Several muscles were placed into one of four functional groups: hamstrings, quadriceps, dorsiflexors, or plantarflexors, for statistical comparison of properties between groups. Antagonistic groups (i.e., hamstrings vs. quadriceps or dorsiflexors vs. plantarflexors) demonstrated significant differences in fiber length, fiber length/muscle length ratio, muscle mass, pinnation angle, and number of sarcomeres in series (P〈.02). Discriminant analysis permitted characterization of the “typical” muscle belonging to one of the four groups. The quadriceps were characterized by their large pinnation angles and low fiber length mass ratios, suggesting a design for force production. Conversely, the hamstrings, with small pinnation angles, appeared to be designed to permit large excursions. Similar differences were observed between plantarflexors and dorsiflexors, which have architectural features that suit them for force production and excursion respectively. Although these differences were not absolute, they represented clear morphological distinctions that have functional consequences.
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  • 72
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    Journal of Morphology 199 (1989), S. 125-150 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The larva of the ascophoran cheilstome Watersipora arcuata is described on the basis of serial 1-μ sections, light microscopy of whole mounts, and scanning electron microscopy. Using lightly osmicated specimens, it was possible to map almost every cell on the larval surface. Limited observations on hatching and larval behavior are provided in conjunction with the anatomical description. Tissues of the larva are partitioned between those that function exclusively during the larval period and are degraded at metamorphosis as transitory tissues and those that will have postmetamorphic fates in formation of the ancestrula. Significantly, W. arcuata has two possible anlagen for the ancestrular polypide, the infracoronal cells in the oral hemisphere and the epidermal blastemal cells in the aboral hemisphere, rather than only one or the other of these as reported in other species. Also detailed are the supracoronal flange and groove, which are unique to this genus and are involved in the transmission of mycoplasma-like organisms between successive generations of adults; two pairs of complex pigment cup ocelli; multiple intercoronal cells that are presumed to have varied sensory and mechanical functions; and the sensory, adhesive, and locomotory components of the pyriform organ. The larval anatomy of W. arcuata is compared with that of the larvae of the ctenostomes Alcyonidium gelatinosum (coronate), Bowerbankia imbricata (coronate), B. gracilis (coronate), and Flustrellidra hispida (shelled lecithotrophic) and of the cheilostomes Bugula neritina (coronate), Electra pilosa (cyphonautes), and Membranipora membranacea (cyphonautes). This study is the first detailed analysis of the larval structure of any ascophoran bryozoan and provides a necessary platform for subsequent analyses of embryology and metamorphosis.
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  • 73
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    Journal of Morphology 199 (1989), S. 175-196 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The embryonic development of the hemipteran insect Rhodnius prolixus was studied by use of contemporary light and electron microscopy. Embryos were staged according to days postoviposition. Eggs laid on day one complete blastoderm formation and anatrepsis, the first phase of blastokinesis, by day 5. The embryo develops in a cephalocaudal orientation which is 180° to the anteroposterior axis of the egg. Subsequent development, prior to the second phase of blastokinesis (katatrepsis), leads to segmentation of the germ band, evagination of appendages, and histogenesis of germ layers. Concomitantly with these events, the amnion undergoes dramatic change. By day 7 the embryo begins a 180° revolution while migrating to the ventral surface of the yolk. This restores its polarity with respect to that of the egg and facilitates hatching. The serosa contracts, pulling the amnion and embryo anteriorly. Eventually the serosa is internalized at a point dorsal to the head and the lateral walls of the embryo grow up and surround the yolk. Development continues until day 15 when the embryo hatches as a first instar larva.
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  • 74
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    Journal of Morphology 199 (1989) 
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  • 75
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    Journal of Morphology 199 (1989), S. 287-297 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Morphological and physiological analyses confirm that the semitendinosus muscle of goats contains two separate compartments in series, each with distinct innervation. These compartments of the muscle are in turn composed of short fibers (approximately four fibers in series in the proximal compartment and seven to eight fibers in the distal compartment) which overlap each other for more than 30% of their length, with much of the overlapping portions consisting of slender tails that terminate at one-tenth of the midfiber diameter. Groups of fibers are associated into relatively narrow bands that run end-to-end in each compartment. The data suggest that the maximum length of muscle fibers may be limited; even the fibers of parallelfibered muscles may not scale with the dimension of the animal.
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  • 76
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    Journal of Morphology 199 (1989), S. 349-361 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of the 51/2-6-day-old embryonic asteroid basal lamina (BL) was studied by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and after treatment with anionic dyes. Conventional fixation in glutaraldehyde and osmium reveals a BL consisting of a lamina densa separated from the basal cell surface by a lamina lucida. Little or no reticular lamina is present. Material similar in appearance to the basal lamina extends into the blastocoel, forming an extracellular matrix (ECM). Following fixation in the presence of the dye ruthenium red, proteoglycan (PG) granules are visible in the lamina lucida and immediately beneath the lamina densa. The ECM consists of granules of a similar appearance, which are associated with fibers of an intermediate electron density resembling invertebrate collagen. After fixation in the presence of alcian blue under polyanionic conditions, all aspects of the basal lamina and the ECM stain very densely. The use of alcian blue in 0.3 M MgCl2 (monoanionic condition) or in low concentrations reveals a lamina densa consisting of a fine feltwork and tubule-like structures. A meshwork composed of thick, densely stained and thinner, intermediately stained strands is embedded in the inner aspect (that adjacent to the blastocoel) of the ectodermal lamina densa. Similar elements are present in the endodermal BL, but the dense material is represented by short regions that do not form a meshwork. The dense and intermediate strands of both basal laminae also extend into the blastocoel as ECM. The tubule-like structures extend from the dense material of the inner meshwork into the lamina densa. They also cross both the lamina densa and lucida to associatee with the basal cell membranes. The fact that the basal cell surfaces are often puckered outward at the points of contact suggests that this configuration might be providing a means whereby forces can be transferred from the ECM through the basal lamina to the cells.
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  • 77
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    Journal of Morphology 200 (1989), S. 17-27 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Epithelial and cuticular linings of the stomach investigated in three species representing different genera of the Mysidacea are elaborated into a set of structural specializations dividing the stomach longitudinally into one dorsal and two ventral channels. The dorsal, or food, channel contains ingested food and retains coarse particles, which eventually are transported into the midgut through a funnel. The ventral, or filtration, channels, which are separated by an anterior and a posterior median ridge (anteromedianum, inferomedianum), contain fine particles and soluble materials extracted from the dorsal channel through two filter systems: primary filters, which lie anteriorly on either side of the anteromedianum, and posterior secondary filters, which are located on the inferomedianum. The final filtrate is transported into the ventral caeca or midgut glands. The ultrastructure of the cuticle lining the lumen of the stomach shows several specializations, the most prominent of which are stout spines and delicate filter devices. The epithelium is multilayered in circumscribed areas (the lateralia). The basement lamina is extremely developed in the inferomedianum. Detailed knowledge of the microscopic anatomy and the ultrastructure of the stomach allows identification of several homologous gastric structures among different peracaridean groups and in Decapoda.
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    Journal of Morphology 200 (1989), S. 71-86 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In primitive eutherians, the stapedial artery is the primary supplier of blood to the nonneural tissues of the head. Beyond a certain body size, the stapedial artery can no longer function as the sole supplier to its original territory because the diameter of its stem is limited by the size of the intercrural foramen of the stapes, which exhibits strong negative allometry. Some eutherians have extended the upper limit that the diameter of the stapedial stem can attain by developing a coarctation (narrowing) at the transcrural portion of the vessel. In the Norway rat (Rattus norvegicus) and the golden hamster (Mesocricetus auratus) the coarctation develops in postnatal life and is evidently caused by a retardation in growth that keeps the diameter of the vessel at infantile dimensions. In the rat, additional reduction in the external diameter is produced by a thinning of the tunica media of the arterial wall. A comfortable gap between the wall of the artery and the sides of the intercrural foramen is maintained that most likely facilitates the attenuation of potentially disruptive low-frequency vibrations produced by the arterial pressure pulse. The only negative side effect of a coarctation in rat-sized animals is that resistance to flow is increased and volume flow rate is concomitantly diminished. The coarctation does not create flow disturbances downstream of the constriction. One possible additional benefit of the coarctation is a flattening out of the arterial pressure pulse. It is speculated that the capacity to develop a coarctation once a certain body size is reached is an ancient trait that dates at least as far back as the Early Cretaceous.
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    Journal of Morphology 200 (1989), S. 93-119 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structure of the organ of Bellonci indicates that it is a sensory organ able to receive a variety of external stimuli. The suspension of the organ was investigated in the marine amphipod Gammarus setosus by light, scanning, and transmission electron microscopy. The organ is suspended ventrally by a cord whose specialized cuticular attachment is in the roof of a canal under the interantennal angle of the head. The conical end of the cord consists of five epithelial attachment cells sheathed by processes of 21 cells arranged in five tiers inside a basal lamina. The attachment cells end within a knob in the centre of a diaphragm. The cuticular attachment is confined to the knob and consists of endocuticular rods within pits on the surfaces of the attachment cells. Dorsally, the long processes of the attachment cells form a net over the surface of the organ and reunite in the dorsal suspensor, which anchors the organ in the epidermis at the crown of the head. The attachment cells are characterized by extensive hemidesmosomes and dense core granules at both attachment sites and by microtubules that extend through their entire length of up to 1 mm in the adult. The large size, cephalic position, elaborate structure, and suspension of the organ suggest that it is of considerable importance in the sensory capability of aquatic Malacostraca.
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  • 80
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    Topics: Biology , Medicine
    Notes: Adult male marbled newts (Triturus marmoratus) were collected at the beginning of the spermatogenetic period and exposed to different photoperiods (natural photoperiod with progressively increasing daylengths, total darkness, 8L:16D, 12L:12D, 16L:8D, and continuous light) for 3 months at 20°C. To evaluate the effect of photoperiodic input via pineal gland photoreceptors, two additional groups of newts were blinded by a non-aggressive method (an elastic rubber cap was adjusted to the head to cover the eyes but not the pineal photoreceptors). These animals were exposed either to the natural photoperiod or to 12 hr of light per day. Quantitative histologic studies on testicular development and germ-cell volume revealed no significant differences between non-blinded and blinded animals. Testicular size and germ-cell development increased in the following order: total darkness, constant light, 8L:12D, natural photoperiod, 12L:12D, and 16L:8D. These results suggest that (1) long photoperiods enhance testicular development, whereas short photoperiods or an environment of continuous light have the opposite effects and (2) the effect of photoperiods on testicular function in newts is independent of the ocular photoreceptors.
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    Journal of Morphology 199 (1989), S. 259-268 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Development of the gastric connective tissue of Xenopus laevis during metamorphosis was investigated by electron microscopy. Throughout the larval period to stage 60, the layer of connective tissue underlying the gastric epithelium consists of immature fibroblasts surrounded by a sparse extracellular matrix. At the beginning of the transition from the larval to the adult epithelial form, at about stage 60, extensive changes occur in the connective tissue. The number of cells suddenly increses and different cell types appear. Numerous contacts between epithelial and connective tissue cells are established through random gaps in the thickened basal lamina. During stages 62-63, just after the beginning of the morphogenesis of adult-type glands, the basal lamina lining the glandular epithelium becomes thinner, and the number of contacts decreases rapidly except near the tips of the glands. After the glandular cells begin to produce zymogen granules at stage 64, contacts become rare. From stage 63, when the muscularis mucosae develops, until the completion of metamorphosis, the connective tissue consists mainly of typical fibroblasts. Outside the muscularis mucosae, the fibroblasts of the lamina propria are aligned in parallel with the curvature of the glands. These observations indicate that developmental changes in the connective tissue are closely related spatiotemporally to those of the epithelial transition from larval to adult form during metamorphic climax. Although some changes are similar to those in the intestine (Ishizuya-Oka and Shimozawa, '87b), others are specific to the gastric region, which suggests that connective tissue may have a role in organ-specific differentiation of the gastric epithelium.
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    Journal of Morphology 199 (1989), S. 313-325 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: To our knowledge, this paper presents the first description of neuroendocrine complexes in invertebrates. Neuroendocrine complexes have been identified in the lamina propria of the snail gut by light microscopy using Giemsa-stained, deplasticized sections and by electron microscopy. Silver impregnations and immunocytochemical tests for 15 different peptide hormones yielded negative results. The ultrastructure of the snail neuroendocrine complexes is basically similar to that of neuroendocrine complexes in vertebrates. They are composed of endocrine cells and associated unmyelinated nerve processes, both being surrounded by a discontinuous glial sheath and a basement membrane. The endocrine cells contain small (139 nm), round, dense-cored granules. The presence of complex multivesicular bodies and autophagic vesicles in the endocrine cells suggests that excess granules may be eliminated by a process of crinophagy. Some of the neuroendocrine complexes were seen to form connections with the epithelium by means of an endocrine cell cytoplasmic process, a fact that strongly suggests a functional integration between the intestinal epithelium and the enteric nervous system.
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  • 83
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    Journal of Morphology 199 (1989), S. 327-347 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Water drinking in the mallard is accomplished by a fine-tuned set of movements of upper and lower jaw and of the tongue. During immersion of the tips of the bill, the oral cavity is formed into smaller volumes containing water and into connecting tubes. Two mechanisms serve the water transport: (1) lingual and jaw movements press water from the water-containing spaces into the tubes; (2) a quantitative simulation of the shape of the oral cavity during immersion shows that the two tubes are so narrow that capillary action also contributes to water transport.Thereafter, the tips of the bill are raised until they point upward. In this “tip-up” position, water flows into the esophagus because of gravity. We conclude that, in addition to normal tip-up drinking observed in almost all Passeriformes and Galliformes, a second type of tip-up drinking may be distinguished in Anseriformes. The integration of the drinking mechanism, keeping the water inside the mouth, and the straining mechanism, expelling the water along the beak rims, is effected by specific actions of the elaborate lingual apparatus.
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  • 84
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    Journal of Morphology 199 (1989), S. 379-384 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Tubules containing sperm were identified by light microscopy in the oviducts from 11 species of turtles representing six different families. Sperm storage tubules were found in a small region of the posterior portion of the egg albumin-secreting section of the oviduct located between the infundibulum and the uterus. This location of storage tubules, midway between the ovary and vagina, is unique among vertebrates. Ducts, restricted to the posterior albumin region, connect the tubules to the oviduct lumen, allow entrance of sperm to the tubules. Sperm were identified in tubules of female turtles isolated from males for as long as 423 days.
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  • 85
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The antennal gland of the crayfish Pacifasticus leniusculus was studied using standard techniques for scanning electron microscopy as well as newer procedures for ultrasonic microdissection. To clarify relationships in the nephron tubule, transmission electron microscopy was employed.The coelomosac contains elongated cells (podocytes) displaying microvilli and extensive apical blebbing. A smooth basal lamina lines the blood space that furnishes hemolymph to the coelomosac. The labyrinth consists of tall columnar cells displaying apical microvilli, numerous blebs that seem to represent an expansion of apical plasma membrane, and lateral interdigitations. The nephron tubule consists of two distinctly different areas: a proximal region of flattened cells with extensive intercellular fusions, and a distal segment of separate, dome-shaped cells.Despite many similarities between the crayfish kidney and the vertebrate nephron, there are striking differences. The amount of surface blebbing that occurs in the coelomosac and labyrinth far exceeds that of the vertebrate nephron and may reflect its importance in the function of the crayfish kidney. The cells of the coelomosac are taller than are the vertebrate podocytes and possess less obvious arms and pedicels. In addition, the proximal segment of the nephron tubule is notable for its intercellular fusions, which are not present in the vertebrate nephron. Although the function of the intercellular fusions is unknown, they may play a role in cellular communication or the redistribution of fluids or electrolytes between cells.
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  • 86
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    Journal of Morphology 201 (1989), S. 235-242 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Light and electron microscopic examination of the dorsal lingual epithelium of beagle dogs (Canis domesticus) revealed three different regions: that anterior to the filiform papillae, that posterior to the papillae, and an interpapillary region. Whereas the basal and suprabasal cells are similar throughout, differences characterize the intermediate and surface layers. Keratohyalin granules are common in the intermediate layers in the anterior and interpapillary regions, tonofibrils are prominent in the posterior region, and no keratohyalin granules occur. The surface layer of the interpapillary region is not keratinized, that of the anterior region shows soft keratinization, and that of the posterior region shows hard keratinization. The perimeter of keratohyalin granules is composed of ribosomes 10-20 nm in diameter.
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  • 87
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    Journal of Morphology 201 (1989), S. 273-284 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Based on light microscopic observations, nerve pathways are described for the first time in the praesoma of a species of the Echinorhynchidae. The pathways are described for 18 nerves, 8 paired and 2 single, which originate from the cerebral ganglion and a post-ganglionic cell and terminate in the body wall musculature and the proboscis. The location of two commissures formed by these nerves is also described.
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  • 88
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    Journal of Morphology 201 (1989), S. 253-272 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ontogeny of the gut in Penaeus setiferus was investigated by reconstruction of serial sections examined by light microscopy. Development of the gut into the adult form is protracted over several weeks beyond metamorphosis in steps that may be directly related to the unique postlarval life history of Penaeus. The gastric mill is lacking in larval stages of P. setiferus. In protozoeal stages Z1-Z3, the pyloric ampullae are blind sacs that do not communicate with the midgut. The gland filter first appears in mysis stage M2. The gastric mill in early postlarval (PL) stages consists of poorly chitinized lobes with flexible setae. By PL21 the ossicles of the gastric mill are rigid and setae are replaced by spine-like denticles, but even by PL35 the gastric mill is neither as massive nor heavily chitinized as in adults. During the mysis stages and early PL stages, the hepatopancreas communicates freely with both the foregut and the midgut trunk. By PL35 the hepatopancreatic ducts are essentially isolated from the remainder of the midgut by foregut ossicles.The midgut in Z1 consists of two pairs of simple caeca and the midgut trunk. During larval growth, each of the lateral midgut caeca develops into a number of lobes. After metamorphosis these lobes begin to ramify into small-diameter tubules, and by PL35 have completely ramified into the hepatopancreas of adults. From M1 to PL4, the anterior midgut caeca decrease in absolute size and become a single anterior diverticulum. The posterior midgut diverticulum first appears in PL21 as a simple sac and thereafter increases in size and complexity.
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  • 89
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    Journal of Morphology 201 (1989), S. 293-299 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The sexually dimorphic vocal characteristics of Rana pipiens release calls suggest that there may be differences in the anatomical components of the larynx. The volumes of the arytenoid cartilage, surrounding muscle masses, vocal cords, supporting bronchial process, and the release-call amplitudes of six males and five females were measured in same-sized animals and sexual differences assessed. No qualitative differences in laryngeal morphology were observed, but all features measured except vocal cords were significantly larger in males. The implications of an increased laryngeal size are discussed in relation to differences previously observed in the vocalizations of this and other species and in relation to prior suggestions regarding the developmental basis of anuran sexual dimorphisms.
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  • 90
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    Journal of Morphology 201 (1989), S. 315-329 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The simultaneous use of electromyography (EMG), strain gauges, and cinematography show that the capacity of continuous displacement from a single peg is based on the following: sequential activity of the tested muscles from front to rear; activity restricted to the short portion of the body in contact with the peg; alternate action of the muscle longissimus dorsi on the two sides, the transition between one side to the other occurring at the site of contact with the peg; unilateral activity of the muscle supracostalis ventralis responsible for a bulging against the peg; a great stability in the direction of the resultant force, which makes only a small angle with the directio of the motion.
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  • 91
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    Journal of Morphology 201 (1989), S. 11-22 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The sequence of morphological differentiation of Müller cells in the chick retina was investigated in relation to the differentiation of the retinal neurons using the Golgi method. From the beginning of differentiation, the Müller cell develops spurs and lateral processes. Some of these glial processes become transformed into accessory prolongations of the Müller cell. From the 17th or 18th day of incubation, the morphology of the Müller cells is similar to that of the adult retina. On the basis of their inner prolongation, two types of Müller cells were identified. The first type, with diffuse and abundant descending processes, is identical to that described classically. The second type is a cell characterized by sparse and scanty inner ramifications.This report also describes electron microscopic observations of Müller cells and their enwrapping relationship with the axons of the optic nerve fiber layer.
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  • 92
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    Notes: The ovotestis of Spurilla neapolitana consists of a series of spherical lobes, each of which is composed of radially arranged, sac-like acini or follicles. The male and female portions of each acinus are separated by ovarian follicle cells and testicular accessory cells. A thick basal lamina serves as a barrier between adjacent acini. The surface of each ovotestis lobe is covered by several layers of myoepithelial cells resting on a connective tissue layer. Developing oocytes are intimately associated with follicle cells except in the last stages of vitellogenesis. Follicle cells are characterized by the presence of extensive arrays of rough endoplasmic reticulum (RER) and Golgi complexes and may play a role in vitellogenesis. An ultrastructural analysis of vitellogenesis suggests that oocytes utilize both auto- and heterosynthetic mechanisms of yolk formation. Autosynthetsis is suggested by the activity of the Golgi complex and RER, while heterosynthesis is indicated by high levels of endocytotic activity by the oocyte. Follicle cell development and high endocytotic activity in the oocytes may be a reproductive adaptation to accelerate yolk synthesis, resulting in more rapid egg production.
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  • 93
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    Journal of Morphology 201 (1989), S. 285-291 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Each of the 34 nephridia in the leech, Hirudo medicinalis, has its own separate bladder. Urine flows from the last portion of the nephridium, the final canal, into the bladder through a special inlet which prevents backflow of urine. This inlet consists of a vestibule formed by two serially arranged septa, each with a small pore. As no muscles or cilia are associated with either the nephridia or the bladder inlet, urine flow into the bladder is passive. Urine leaves the bladder through an outlet that consists of a urethra with sphincters at both ends and an opening, the nephridiopore, in the ventral skin. The sphincter muscles are distinct from the body wall muscles and receive double innervation: urine retention and release is therefore active.
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  • 94
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    Journal of Morphology 201 (1989), S. 301-314 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Scanning electron microscopy of postnatally developing gerbil vagina (birth to maturity) shows that longitudinal folds form prior to transverse folds; the process of fold formation is initiated on the dorsal wall and proceeds ventrally. From days 1 to 7 postnatally, the vaginal epithelium is composed of either flat or bulging cells, depending on the vaginal region. The luminal cell surface is covered with uniform stubby microvilli and solitary cilia. Between days 9 and 20, the flat cells with distinct cell boundaries spread toward more proximal areas, leading to the formation of mixed patches of cells with flat or rounded apices. Individual elongated microvilli or tufts of forked microvilli may sprout from their surfaces. Solitary cilia gradually disappear.The transition from immature to mature vaginal epithelium starts around day 20, when individual cells recess below the level of neighboring cells. This process spreads throughout the vagina during the following days, reflecting local changes in the subsurface layers of the epithelium preparatory to exfoliation. Around day 40 the actual exfoliation of the luminal cell layer starts. By this time the surface characteristics of many of the desquamating cells have changed. In addition to microvilli, microridges are being formed. The process of exfoliation is finished by about day 60. The newly appearing cell layers now transform into typical cornified cells of the cycling vaginal epithelium.
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  • 95
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    Journal of Morphology 202 (1989), S. 29-51 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The developing skeleton of a pelobatid frog, Spea bombifrons, is described based on a series of 81 cleared-and-stained tadpoles and juveniles. Most limb and limb-girdle elements commence ossification premetamorphically, along with the parasphenoid, frontoparietals, exoccipitals, and vertebral column, during Gosner Stage 36. The chondrocranium undergoes dramatic restructuring at the beginning of metamorphosis (ca. Stage 40); and the nasal cartilages, prootics, premaxillae, nasals, maxillae, septomaxillae, and ischium appear. Near the end of metamorphosis (ca. Stage 44), the branchial arches are resorbed, the hyoid plate and quadrate form, and the angulosplenials, vomers, squamosals, dentaries, and pterygoids ossify. After metamorphosis (Stage 46), the laryngeal cartilages, sternum, omosternum, and plectral apparatus chondrify; and the carpals, tarsals, sphenethmoid, posteromedial hyoid processes, mentomeckelian bones, quadrates, columellae, and opercula ossify. The development of the fused sacrococcygeal articulation in S. bombifrons is described and compared to the more widespread bicondylar articulation. The presumed palatine bone and transverse processes of the coccyx are discussed, as are several seemingly paedomorphic skeletal features of Spea. Comparison with the sequence and timing of ossification in other anurans reveals that ossification of the skull and vertebral column occurs later in S. bombifrons than in other anurans; nonetheless, many aspects of the ossification sequence seem to be shared by a surprisingly wide range of anuran taxa.
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  • 96
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    Journal of Morphology 202 (1989), S. 89-98 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The climbing behavior, muscle mechanics, and functional properties of selected forelimb muscles were examined to ascertain how three distantly related mammals may be adapted for climbing. To determine if features of the fox squirrel (Stalheim-Smith: J. Morphol.180:55-68, apos;84) are general or unique features for a climber, two distantly related climbers, the raccoon (Procyon lotor) and the opossum (Didelphis virginiana), were studied. Muscle mechanics varied: the elbow flexors of the fox squirrel produced significantly more torque per unit mass than did the corresponding muscles of the opossum except at 80°, but not more than the corresponding muscles of the raccoon. On the other hand, there were no statistically significant differences in torque per unit mass among the elbow extensors of the three climbers. Both elbow flexors and elbow extensor had faster contraction times and were more fatigable in the fox squirrel than in the opossum or in the raccoon. The data suggest that the musculoskeletal characteristics of the forelimbs of climbers vary according to behavioral, and possibly phylogenetic, differences.
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  • 97
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    Journal of Morphology 202 (1989), S. 151-164 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of five epithelial cellular types is described. (1) Absorptive-storing cells possess a striated border and accumulate lipids and glycogen. They also contain an apical multivesicular complex and numerous membrane-bound PAS-positive granules of unknown significance. (2) Zymogenic cells contain numerous, large secretory granules. These cells also possess microvilli and accumulate lipid droplets. (3) Current-producing cells contain bundles of thick and thin myofilaments and extend from the basal lamina to the lumen. (4) Mucous cells display the characteristic features of such secretory cells. (5) In addition to these four cellular types, already observed under the light microscope by other authors, endocrine cells have also been identified by both light and electron microscope. They contain variable amounts of secretory granules of diverse sizes and electron densities. Immunocytochemical studies reveal the presence of cells immunoreactive to somatostatin, glucagon, and pancreatic polypeptide. Intraepithelial nerve fibers in contact with endocrine cells are also present. As far as we know, this appears to be the first description of enteroendocrine cells in the phylum Echinodermata.
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  • 98
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    Journal of Morphology 202 (1989), S. 129-150 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The feeding mechanism of Epibulus insidiator is unique among fishes, exhibiting the highest degree of jaw protrusion ever described (65% of head length). The functional morphology of the jaw mechanism in Epibulus is analyzed as a case study in the evolution of novel functional systems. The feeding mechanism appears to be driven by unspecialized muscle activity patterns and input forces, that combine with drastically changed bone and ligament morphology to produce extreme jaw protrusion. The primary derived osteological features are the form of the quadrate, interopercle, and elongate premaxilla and lower jaw. Epibulus has a unique vomero-interopercular ligament and enlarged interoperculo-mandibular and premaxilla-maxilla ligaments. The structures of the opercle, maxilla, and much of the neurocranium retain a primitive labrid condition. Many cranial muscles in Epibulus also retain a primitive structural condition, including the levator operculi, expaxialis, sternohyoideus, and adductor mandibulae. The generalized perciform suction feeding pattern of simultaneous peak cranial elevation, gape, and jaw protrusion followed by hyoid depression is retained in Epibulus. Electromyography and high-speed cinematography indicate that patterns of muscle activity during feeding and the kinematic movements of opercular rotation and cranial elevation produce a primitive pattern of force and motion input. Extreme jaw protrusion is produced from this primitive input pattern by several derived kinematic patterns of modified bones and ligaments. The interopercle, quadrate, and maxilla rotate through angles of about 100 degrees, pushing the lower jaw into a protruded position. Analysis of primitive and derived characters at multiple levels of structural and functional organization allows conclusions about the level of design at which change has occurred to produce functional novelties.
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  • 99
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    Journal of Morphology 202 (1989), S. 179-184 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The post-hatching growth of the kidney of the chukar partridge, Alectoris chukar, was studied in birds aged 5, 24, 56, and 140 days. As body mass increased from 24 gm (5 d) to 479 gm (140 d), kidney mass increased from 0.16 to 1.6 gm. The number of nephrons in the kidney increased through age 56 d, but did not change thereafter. This increase was primarily a function of increasing development of the cortical regions; more than 90% of reptilian-type nephrons developed after hatching. The full complement of medullary cones was present at hatching. However, the lengths of the medullary cones and the number of loops of Henle of mammalian-type nephrons contained in each medullary cone increased through growth, so that approximately 65% of the mammalian-type nephrons developed after hatching. The chukar kidney contained 19% mammalian-type nephrons at age 5 d, but this was reduced to approximately 5% by 24 d, after which the proportions of mammalian-and reptilian-type nephrons did not change. This study demonstrates that metanephric kidney growth in birds begins in the deepest regions of the kidney and then extends peripherally but also that mammalian-type nephrons continue to develop throughout kidney growth, presumably from pre-existing reptilian-type nephrons.
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  • 100
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    Journal of Morphology 199 (1989), S. 15-22 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A previously unidentified intercellular junction between cone cells in the compound eye of the moth Ephestia is described. The junctions are characterized by deposition of granular material, in register, along portions of the membranes of adjacent cone cells during compound eye development and by a constant intercellular space of 8-10 nm. Accumulation of the cone cell material along localized regions of the cell membrane suggests an interaction between a specialized area of the membrane and a specific cytoplasmic constituent, and the exact matching of the regions of deposition between adjacent cells implies intercellular interaction. The junctional nature of these membrane regions is often obscured in the adult crystalline cone but is inferred from observations on developing cone cells.
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