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  • Articles  (70)
  • Oryza sativa  (70)
  • Animals
  • Chemical Engineering
  • 1990-1994  (70)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (70)
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  • Articles  (70)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 76 (1994), S. 139-143 
    ISSN: 1573-5060
    Keywords: bacterial brown sheat rot ; germination ; Oryza sativa ; Pseudomonas fuscovaginae ; rice ; screening for toxin tolerance ; seedling growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of a Pseudomonas fuscovaginae toxin, on germination, root formation and seedling elongation, after soaking rice grains in the toxin prior to sowing, was investigated. The toxin enhanced germination, but had no apparent effect on the number of roots of the seedlings. It induced a drastic inhibition of seedlings elongation correlated to varieties susceptibility to the disease in the rice field. After denaturation of the bioactive compounds of the extract, all the previously observed effects were lost. Using the toxin and the present test, could be a reliable tool for screening genotype susceptibility to P. fuscovaginae disease.
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  • 2
    ISSN: 1573-5060
    Keywords: gametogenesis ; mutagenesis ; radiosensitivity ; mutation frequency ; X-ray irradiation ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Developmental stages during gametogenesis of rice were histologically examined in the period from differentiation of reproductive organs to anthesis. Plants were exposed to acute X-rays of 20 Gy. Radiosensitivity and mutation frequency were investigated in relation to the developmental stages of reproductive organs. The most radiosensitive stage, as measured by reduction of the M1 pollen-and seed-fertilities, was the last premeiotic interphase. Mutations induced at different developmental stages were scored in M3 strains. Sterility mutants and short-culm mutants were most frequently observed. Grain shape, panicle morphology, heading-date and endosperm character mutants were induced at a relatively low frequency. The overall mutation frequency varied with the developmental stage at the time of irradiation. The highest overall mutation frequency was observed when radiation was applied 10 days before anthesis, the late tetrad stage of microspores. Radiation exposure of florets at the late tetrad stage was found to be a more efficient method of inducing a large number of mutations than radiations applied to seeds and fertilized egg cells.
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  • 3
    ISSN: 1573-5060
    Keywords: linkage ; molecular markers ; morphological mutant markers ; Oryza sativa ; rice RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The classical genetic map and molecular map of rice chromosome 11 were oriented to facilitate the use of these maps for genetic studies and rice improvement. Three morphological markers (d-27, z-2, and la) were crossed to a rice breeding line, IRBB21, which has the Xa-21 gene for bacterial blight resistance. Three F2 populations were analyzed with RFLP markers known to be located on chromosome 11. Segregation analysis of molecular markers and morphological markers was used to construct an RFLP map for each population. The recombination frequency between markers varied from population to population although the marker order on the maps was the same for all three populations. Based on a common set of markers mapped in the three populations, an integrated map was generated consisting of both RFLP and morphological markers. The genetic distance between markers on this map was determined by taking a weighted average of the data from the three populations. The oriented map serves as a bridge to understand the relationship between the classical and molecular linkage maps. Based on this information, the location of several genes on the classical map can be approximated with respect to RFLP markers without having to map them directly.
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  • 4
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    Plant growth regulation 15 (1994), S. 125-128 
    ISSN: 1573-5087
    Keywords: rice ; Oryza sativa ; phloem sap ; polyamine ; abscisic acid ; auxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Putrescine, spermidine, spermine and cadaverine have been identified and quantified in rice phloem sap and shoot extracts by HPLC. It is suggested that diamines, putrescine and cadaverine, easily migrate into the phloem, while movement of a triamine, spermidine, and a tetramine, spermine, tend to be restricted. Spermine especially seems to be the most immobile among polyamines. Thus it is indicated that movement of polyamines into phloem is decreased with increasing number of amino groups. Indole-3-acetic acid and abscisic acid in rice phloem sap were also analyzed by HPLC and it is suggested that indole-3-acetic acid is transported freely into phloem, while abscisic acid is much more actively exuded into phloem.
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  • 5
    ISSN: 1573-5087
    Keywords: abscisic acid ; cadmium ; gibberellic acid ; mineral nutrition ; nickel ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rice plants accumulate high quantities of Cd and Ni when grown for 10 days in a medium containing these heavy metals. Accompanying Cd and Ni uptake, a decrease in shoot and root length was observed, though dry matter accumulation was not affected accordingly. Metal treatments also induced a decrease in K, Ca and Mg contents in the plants, particularly in the shoots, indicating that Cd and Ni interfered not only with nutrient uptake but also with nutrient distribution into the different plant parts. Addition of abscisic acid (ABA) or gibberellic acid (GA3) to the external solution could not overcome the depressing effects of the metals on nutrient acquisition, and even induced a further decrease of Ca content in Ni-treated plants. Both hormones also reduced, significantly, heavy metal incorporation into the plants. Additionally, hormonal applications affected the transport of Cd and Ni to the shoots, resulting in a higher percentage of the metals taken up remaining in the roots.
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  • 6
    ISSN: 1573-5060
    Keywords: DNA fingerprint ; Lactuca sativa ; Oryza sativa ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Thirty-five rice (Oryza sativa L.) varieties, including 18 japonica, 5 javanica and 12 indica subspecies and 12 lettuce (Lactuca sativa L.) varieties were identified taxonomically, using PCR with originally designed 21 RAPD (Random Amplified Polymorphic DNA) primers and 8 sequence-specific primers, used for amplifying four specific DNA fragments. Use of these primers revealed polymorphisms among varieties in rice and lettuce and facilitates DNA fingerprinting. Dendrograms of both species based on polymorphisms were constructed and genetical relationships were established. In rice, half the number of amplified bands were polymorphic and almost all varieties differentiated. However, differentiation of minor genetic alterations among somaclonal variants or mutants and their mother varieties was not feasible. In L. sativa, 47% of the amplified fragments were polymorphic and all 12 varieties were differentiated. Some of the PCR fragments were variety or type specific, which could be used for indicators for type-selection. The dendrogram obtained showed differentiated clusters of crisphead, leaf and butterhead type, findings in good accord with the classification based on the genetic background.
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  • 7
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    Euphytica 76 (1994), S. 89-96 
    ISSN: 1573-5060
    Keywords: Indica rice ; Oryza sativa ; radiation mutagenesis ; thermo-sensitive male sterility ; pollen ; vigour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Male-sterile mutants were isolated from M2 and M3 generations of indica rice variety 26 Zhaizao, dry seeds of which had been exposed to 60Co-γ rays at a dose of 290 Gy. The mutants were planted in early season and ratooned in late season for two successive years for identification of fertility conversion in different growing seasons. One of the mutant lines was further observed in a growth chamber and in the field. Results showed that daily average temperature might be the major factor conditioning the male fertility conversion at a moderate daylength. The critical temperature for the male fertility conversion of the mutant grown under 12.5 h and 14.0 h daylength is about 23°C, below which the plant becomes completely male sterile. Its male fertility conversion character differs from other EGMS lines so far developed. The performance of the hybrids between the mutant and some other indica varieties demonstrated its good combining ability and its potential value in hybrid rice production. The obtained mutant line still sheds KI-stainable pollen grains under male sterilizing conditions. Nevertheless, pollen grains shed from the male sterile plants were much more vulnerable than from normal plants. At sucrose concentration below 1.5 M, the pollen grains from the mutant grown under male sterilizing conditions almost completely broke down, while above 1.5 M they became plasmolysed and shrunken. This is indicative of poor development of the membrane and walls of the pollen grains from the male sterile mutant, causing the pollen grains to be unfunctional. NBT test also clarified the abortion of the pollen grains from the mutant, which were formed in the male sterilizing environment.
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  • 8
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    Plant growth regulation 15 (1994), S. 83-92 
    ISSN: 1573-5087
    Keywords: amine conjugates ; arginine decarboxylase ; Oryza sativa ; polyamine inhibitors ; polyamines ; seedling development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The main free amines identified during growth and development of rice seedlings were agmatine, putrescine, spermidine, diaminopropane and tyramine. Amine composition differed according to tissue and stages of development. Conjugated amines were only found in roots. We present evidence that arginine decarboxylase (ADC) regulates putrescine during the development of rice seedlings. When ADC action was blocked by DFMA (α-DL-difluoromethylarginine, a specific irreversible inhibitor of ADC), polyamine titers and seedling development were diminished; when agmatine or putrescine was added, normal polyamine titers and growth were restored. The effects of DFMA were concentration dependent. DFMO (α-DL-difluoromethylornithine, a specific irreversible inhibitor of ornithine decarboxylase or ODC) promoted growth and development at concentrations below 2 mM. This effect was probably related to its unexplained, but consistently observed slight enhancement of rice ADC. When the increase in the concentration of spermidine was prevented by CHA (cyclohexylammonium sulfate), the number of roots increased and the increase in length of leaves and roots was strongly inhibited. The addition of exogenous spermidine at the time of treatment with CHA reversed the inhibition by CHA.
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  • 9
    ISSN: 1573-5087
    Keywords: 1-aminocyclopropane-1-carboxylic acid ; 1-aminocyclopropane-1-carboxylic acid oxidase ; 2,2′-bipyridine ; ethylene ; 8-hydroxyquinoline ; Oryza sativa ; 1,10-phenanthroline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of metal chelators, 2,2′-bipyridine, 8-hydroxyquinoline and 1,10-phenenthroline, on the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene in detached leaves of light-grown rice (Oryza sativa) seedlings and detached shoots of etiolated rice seedlings were investigated. Metal chelators strongly inhibited the in vivo ACC oxidase activity in detached leaves and detached etiolated shoots. This inhibition could be partially recovered by Fe2+. Our results support the notion that Fe2+ is an essential cofactor for the conversion of ACC to ethylene in vivo.
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  • 10
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    Plant growth regulation 15 (1994), S. 75-82 
    ISSN: 1573-5087
    Keywords: amine conjugates ; arginine decarboxylase ; germination ; Oryza sativa ; polyamine inhibitors ; polyamines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In ungerminated rice seeds, (Japonica rice variety, CV Tapei 309), the content of free amines (putrescine, spermidine, spermine, tyramine) was higher in seed lots having a low germination frequency compared to those with high germination potential. Conversely, amine conjugates (di-feruloylputrescine, di-feruloylspermidine, diferuloyldiaminopropane and feruloyltyramine) decreased with loss of viability. Thus, these compounds appeared to constitute biochemical markers of seed viability. In seeds with high germination potential, conjugates decreased drastically during germination, with an early and rapid increase in free amines (putrescine, spermidine, tyramine). Arginine decarboxylase (ADC) activity was highest during the germination of high germination potential seeds, its activity gradually declining with loss of viability and being closely correlated with agmatine content. The polyamine biosynthetic inhibitors (α-DL-difluoromethylarginine, DFMA, a specific and irreversible inhibitor of ADC; α-DL-difluoromethylornithine, DFMO, a specific irreversible inhibitor of ornithine decarboxylase (ODC); cyclohexylammonium sulfate, CHA, inhibitor of spermidine synthase) neither depleted putrescine and spermidine levels nor inhibited germination in high germination potential seeds. In low germination potential seeds, the germination process was inhibited by DFMA or CHA. Application of agmatine resulted in a reversal of inhibition. DFMA inhibited ADC activity in both categories of seeds. In low germination potential seeds treated with CHA no ADC activity was found. These results suggest that amines are involved in the germination process of rice seeds. It appears that amine conjugates may serve as a storage form of amines which, upon enzymatic hydrolysis, could supply the cell with an additional amine reserve and influence cell division and/or cell elongation.
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  • 11
    ISSN: 1432-0789
    Keywords: Nitrogenase activity ; Acetylene reduction activity ; ARA ; Cyanobacteria ; Soil inoculation ; Wetland rice ; Root system ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse experiment was conducted with wetland rice (Oryza sativa cv. IR-50) in a clay-loam soil (Fluventic Eutrochrept) to study the effect of cyanobacterial inoculation a mixed culture of Aulosira fertilissima, Nostoc muscorum, Nostoc spp., and Anabaena spp., applied at the rate of 0.15 g (dry weight pot-1 or 43 kg ha-1) on acetylene reduction activity in soil and the root system (excised root), and the grain and straw yield. The effects of applying P (40 kg ha-1), N (60 kg ha-1), and P+N to the soil were also evaluated. Cyanbacterial inoculation significantly increased (more than 200% on average) photo-dependent acetylene reduction activity in soils, particularly where the indigenous activity was considerably low, i.e. under unfertilized and N-fertilized conditions. The effect of inoculation was prominent at the maximum tillering and grain formation stages of the crop. This inoculation benefit was, however, marginal in P-applied soils (P and P+N), where the indigenous activity was stimulated more than threefold. The inoculation led to a remarkable increase in root-associative acetylene reduction activity after the maximum tillering stage of the crop, particularly with applied N but for other treatments this inoculation effect was not significant. Cyanobacterial inoculation also increased the grain and straw yield of the crop when N was not applied. The grain and straw yield was significantly correlated with the acetylene reduction activity in flooded soils and in the root system during the tillering and maximum tillering stages of rice growth, respectively.
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  • 12
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    Biology and fertility of soils 16 (1993), S. 151-153 
    ISSN: 1432-0789
    Keywords: Triple cereal cropping ; Barley ; double-cropped rice ; Oryza sativa ; Wetland rice soils ; Stable grain yield ; Organic manuring ; Sustainability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Triple cereal cropping is an important grain production pattern which has been adopted on a vast area of paddy fields in Southern China. A long-term triple cereal cropping (barely with double-cropped rice) experiment has been conducted for 18 years (1974–1991) in paddy fields on the southern coastal plain of Zhejiang. The results showed that long-term triple cereal cropping has a high and stable grain yield level if certain improved crop varieties are used. Furthermore, the use of organic manure to reduce yield fluctuations and promote N-use efficiency was evaluated.
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  • 13
    ISSN: 1573-5060
    Keywords: Oryza sativa ; thermo-sensitive genic male sterile rice (TGMSR) ; critical temperature ; sensitive panicle development stage ; fertility change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of a 1–5 day low temperature treatment applied at different stages during panicle development on the male sterility of 5460S, a thermo-sensitive genic male sterile rice line (TGMSR), was studied. Before and after the period of low temperature treatments the plants were continuously grown under 14.75 h daylength and 29.6° C average temperature (max. 33° C/min. 29° C) in growth chambers in order to avoid any other possible influences on the male sterility. The results indicate that the plant responses to low temperature was dependant on the developmental stage of the young panicle. The stages from formation of pollen mother cell to late uninucleate of pollen grains were the most sensitive to temperature. It is concluded that the critical temperature for the fertility change from male sterile to partially fertile in ‘5460S’ is about 28.5° C in the sensitive panicle development stages under the condition studied.
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  • 14
    ISSN: 1573-5060
    Keywords: direct sowing ; flooded soil ; germplasm ; Oryza sativa ; rice ; seedling establishment ; seedling growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Screening for rice germplasm which can establish seedling from flooded soil was conducted using 256 accessions of conserved germplasm from the International Rice Germplasm Center (IRGC) and 404 accessions from the International Network for Genetic Evaluation for Rice (INGER), IRRI. IRGC germplasm represented broad genetic diversity while INGER germplasm involved desirable agronomic characters. Seeds germinated for 2 d were planted at 25 mm depth in seedling trays. The trays were then submerged to a depth of 30–50 mm. Seedling establishment was evaluated by analyzing leaf development, seedling height, and percentage establishment 15 d after planting. Eight percent and 2% of IRGC and INGER germplasm, respectively, were identified statistically as superior to the control semidwarf IR varieties. Among the superior germplasm were those from Northeast India and Bangladesh which were adapted to deepwater and early summer rainfed lowland cultures. These could be utilized as parents in breeding programs which aim to develop varieties suitable for direct seeding technology (i.e., germinated seeds are sown under the surface of flooded soil).
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  • 15
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    Euphytica 69 (1993), S. 185-190 
    ISSN: 1573-5060
    Keywords: leaf blast ; Magnaporthe grisea ; Oryza sativa ; partial resistance ; Pyricularia oryzae ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In many pathosystems, a prolonged latent period is an important component of partial resistance. Latent period in rice to leaf blast was assessed in cultivars representing a fairly wide range of partial resistance under various conditions that are known to influence the expression of partial resistance considerably. The latent period was slightly more than four days and varied only little between treatments, with a maximum difference of only eight hours between cultivars. The very small differences in latent period were not associated with differences in partial resistance due to cultivar, nitrogen, or leaf age effects. It was concluded that the latent period is of no importance as a component of partial resistance to leaf blast.
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  • 16
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    Euphytica 74 (1993), S. 195-201 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; landraces ; varietal differentiation ; hsien and keng ; indica and japonica ; isozymes ; chloroplast DNA (cpDNA) ; Polymerase Chain Reaction (PCR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ninety Chinese rice landraces were examined with special reference to the indica-japonica differentiation in terms of traditional criteria, isozyme analysis and PCR analysis of the chloroplast DNA (cpDNA). Cultivars were separated into indica and japonica defined by a discriminant function (Z) based on key characters, as well as by isozyme genotypes. Most indica landraces had chloroplast DNAs with a deletion at the Pst-12 fragment, while most japonica landraces had cpDNAs without the deletion. Two traditionally recognized varietal groups in China, keng and hsien, corresponded largely to the respective japonica and indica revealed in our study. The results obtained in this study showed good agreement for classification of indica and japonica types by the three methods: discriminant analysis by Z value, isozyme analysis, and PCR analysis for cpDNA.
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  • 17
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    Plant growth regulation 12 (1993), S. 79-82 
    ISSN: 1573-5087
    Keywords: ethylene biosynthesis ; salicylic acid ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of salicylic acid (SA) on ethylene biosynthesis in detached rice leaves were investigated. SA at pH 3.5 effectively inhibited ethylene production within 2 h of its application. It inhibited the conversion of ACC to ethylene, but did not affect the levels of ACC and conjugated ACC. Thus, the inhibitory effect of SA resulted from the inhibition of both synthesis of ACC and the conversion of ACC to ethylene.
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  • 18
    ISSN: 1573-5060
    Keywords: Porteresia coarctata ; Oryza sativa ; rice ; intergeneric cross ; fluorescent microscopy ; pollen tube ; callose ; postzygotic barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Comparative study with fluorescence microscopy revealed that the pollen tubes of the self-pollinated rice variety BR-9 reached the ovary within 75 min after pollination. In P. coarctata it took 150 min. In the cross between P. coarctata and BR-9, pollen tubes reached the ovary in 165 min but seed setting was not observed. In the cross between BR-9 and P. coarctata, small and deformed pollen tubes were formed and they failed to grow through the stylodium.
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  • 19
    ISSN: 1573-5060
    Keywords: mesocotyl ; diallel cross ; rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mesocotyl length is an important character in promoting seedling emergence of direct-seeded rice. Genetic analysis of rice mesocotyl length was conducted using a six parent diallel cross. Generation mean analysis was carried out on parents (P), F1, F2 and backcrosses (B) of three crosses to complement the genetic information from the diallel analysis. Both analyses demonstrated the presence of significant additive and dominance effects. Duplicate type of non-allelic interaction was detected by the generation mean analysis and two crosses showed significant negative dominance gene effect. Dominance was partial and the narrow sense heritability estimate for mesocotyl length was high, indicating the preponderance of the additive effects. Mesocotyl length was negatively but weakly correlated with the coleoptile length and length of the second internode L2. There was no correlation between mesocotyl length and other mature plant characters such as plant height and internode lengths L1, (L1 being the peduncle with subsequent internodes to the base of the plant). Selection for mesocotyl length can therefore be carried out independent of these plant characters and semidwarf rice varieties with long mesocotyl can be developed.
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  • 20
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    Euphytica 68 (1993), S. 11-16 
    ISSN: 1573-5060
    Keywords: deepwater rice ; elongation ability ; Oryza sativa ; submergence tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study was conducted with six F2 populations to test the possibility of combining submergence tolerance and stem elongation ability into a single genotype of rice (Oryza sativa L.). Submergence tolerance and stem elongation ability could be combined in the same genotype if strongly submergence tolerant genes are present in submergence tolerant parents.
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  • 21
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    Plant growth regulation 13 (1993), S. 133-136 
    ISSN: 1573-5087
    Keywords: cytosolic pH ; Oryza sativa ; putrescine ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of compounds that influenced cytosolic pH on the level of putrescine in detached rice leaves were examined. Permeant weak acids, isobutyric acid and propionic acid, increased the level of putrescine in detached rice leaves. Procaine and trisodium citrate, known to be permeant weak bases, on the other hand, decreased the level of putrescine. It seems possible that the level of putrescine in detached rice leaves is regulated by the cytosolic pH.
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  • 22
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    Plant growth regulation 13 (1993), S. 197-202 
    ISSN: 1573-5087
    Keywords: abscisic acid ; jasmonic acid methyl ester ; Oryza sativa ; osmotic stress ; proline ; putrescine ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of water stress and osmotic stress (sorbitol treatment) on the production of putrescine and proline in excised rice leaves were compared. Osmotic stress and water stress were found to affect differentially the levels of putrescine and proline in excised rice leaves. Putrescine accumulation is induced by osmotic stress, whereas proline accumulation is induced by water stress. The effects of ABA on the levels of proline and putrescine are similar to those of water stress, whereas the effects of jasmonic acid methyl ester (JA-Me) are similar to those of osmotic stress. Water stress results in an increase of endogenous ABA is excised rice leaves. However, neither osmotic stress nor JA-Me has effect on endogenous ABA levels in excised rice leaves. Of particular interest is the finding that proline levels increase when putrescine levels induced by osmotic stress or JA-Me are reduced by D-arginine and α-methylornithine. L-arginine and L-ornithine applied exogenously also cause an increase in proline levels. It seems that L-arginine and L-ornithine are preferentially utilized as precursors for putrescine accumulation in excised rice leaves treated with osmotic stress and JA-Me, and for proline accumulation in excised rice leaves exposed to water stress and ABA.
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  • 23
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    Plant and soil 155-156 (1993), S. 359-362 
    ISSN: 1573-5036
    Keywords: 15N ; microbial biomass ; nitrogen uptake efficiency ; Oryza sativa ; soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recent field studies on irrigated rice at the IRRI research farm indicate efficient use of fertilizer-N based on plant uptake of applied N, (estimated by N difference), and utilization of acquired N for increased grain yield. These findings contrast with 15N uptake in microplot studies which underestimate the actual increase in plant N from added fertiliser. Constraints other than uptake efficiency, however, may govern fertiliser-N efficiency in farmers fields. In a study of 44 farmers' fields in Central Luzon, rice yields ranged from 2.5 to 6.2 t ha-1 and N uptake from 35 to 95 kg N ha-1 in plots without fertiliser-N addition. Farmers applied from 35 to 240 kg N ha-1, but there was no relationship between the N rate used by each farmer and the effective soil N supply. Mean N uptake efficiency from fertiliser by N difference was only 36%. We conclude that improved fertiliser-N efficiency by farmers will require a more information-intensive management strategy that makes N fertiliser inputs better fitted to the seasonal pattern of crop N demand and soil N supply.
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  • 24
    ISSN: 1573-5036
    Keywords: iron ; mineral deficiency ; mitochondrial electron transport ; NADH ; Oryza sativa ; proline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The concentration of proline in shoots of rice (Oryza sativa) seedlings raised in distilled water was about 3.3 times higher than in the seedlings raised in modified B5 medium. The shoots of seedlings raised in B5 medium which was depleted of calcium, iron, magnesium or potassium had a higher concentration of proline than those grown in standard B5 medium. The shoots of seedlings raised in distilled water with iron had a lower level of proline than those in distilled water. These results suggest that iron deficiency leads to high proline accumulation. The electron transport activity of mitochondria from shoots of etiolated seedlings raised in distilled water or iron-depleted B5 medium was significantly lower than those from equivalent seedlings raised with an iron source. As suppression in mitochondrial electron transport leads to an increase in NADH/NAD+ ratio, we propose that the prime cause of the proline accumulation during iron deficiency is to readily maintain NADH/NAD+ ratio.
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  • 25
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    European journal of plant pathology 99 (1993), S. 197-203 
    ISSN: 1573-8469
    Keywords: Pyricularia ozyzae ; Oryza sativa ; respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect ofPyricularia oryzae, the causal organism of leaf blast in rice, on photosynthesis characteristics of rice leaves was measured in two greenhouse experiments. Leaf blast reduced photosynthesis not only through a reduction in green leaf area, but also through an effect on photosynthesis of green leaf tissue surrounding the lesions. The assimilation rate at light saturation (P max ) was more affected than the initial light use efficiency (∈). Dark respiration (R d ) increased as a result of infection. The experimental data were used to derive relations between leaf blast severity andP max , ∈ andR d .
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  • 26
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    European journal of plant pathology 99 (1993), S. 205-217 
    ISSN: 1573-8469
    Keywords: Pyricularia oryza ; Oryza sativa ; simulation model ; respiration ; vertical distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect ofPyricularia oryzae, the causal organism of leaf blast in rice, on photosynthetic rate of a rice crop was determined with mobile equipment in the field. Canopy CO2 exchanges rate (CER) was significantly reduced in plots inoculated withP. oryzae. The experimentally obtained data were used to evaluate the performance of a model for the effects of leaf blast on canopy photosynthesis. The model comprised photosynthesis and respiration routines of a mechanistic crop growth model, extended with submodels for effects of leaf blast on both processes. Canopy photosynthesis and the effect of leaf blast on CER were accurately simulated with the model. Analysis showed that the reduction in canopy photosynthesis was mainly due to an adverse effect of lesions on leaf photosynthetic rate and to shading by dead leaf are resulting from disease induced senescence. A sensitivity analysis demonstrated the importance of the vertical distribution of the disease in the canopy. This implies that disease monitoring for crop loss assessment should consider vertical disease distribution.
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  • 27
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    European journal of plant pathology 99 (1993), S. 323-334 
    ISSN: 1573-8469
    Keywords: Pyricularia oryzae ; Oryza sativa ; crop loss
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rice crops grown under irrigated conditions were inoculated withPyricularia oryzae during early growth stages to study the effect of leaf blast on yield formation. The inoculations led to severe epidemics of leaf blast around maximum tillering, characterized by the presence of typical blast lesions and an accelerated senescence of heavily infested leaf tissue. Leaf blast led to a prolonged tillering and a delay in flowering and maturity. Crop growth rate and leaf area formation declined sharply during establishment of the disease and continued to be reduced till maturity. This resulted in a marked reduction of total dry matter production and grain yield. Dry matter distribution was not affected. Leaf blast reduced spikelet number, 1000 grain weight, and the fraction filled grains. From this last observation it was concluded that the reduction in grain yield was exclusively source determined. Nitrogen uptake of the inoculated crops before flowering was reduced compared to the N uptake of the control crop, but shoot N content of the inoculated crops at flowering was higher. Uptake of nitrogen after flowering was negligible in both healthy and inoculated crops. Redistribution from vegetative tissue was therefore the main source of N for grain growth. The higher N content of the shoot organs in the inoculated crops during ripening led to the conclusion that the reduced N uptake was not responsible for the yield reduction observed. Consequently, the reduction in grain yield was solely determined by a reduced carbohydrate supply.
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  • 28
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhizae ; Inorganic nutrients ; Plant growth ; Pretransplant ; Fertilization ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of P, N and Ca+Mg fertilization on biomass production, leaf area, root length, vesiculararbuscular mycorrhizal (VAM) colonization, and shoot and root nutrient concentrations of pretransplant rice (Oryza sativa L.) plants were investigated. Mycorrhizal plants generally had a higher biomass and P, N, K, Ca, Mn, Fe, Cu, Na, B, Zn, Al, Mg, and S shoot-tissue nutrient concentrations than non-mycorrhizal plants. Although mycorrhizal plants always had higher root-tissue nutrient concentrations than non-mycorrhizal plants, they were not significantly different, except for Mn. N fertilization stimulated colonization of the root system (colonized root length), and increased biomass production and nutrient concentrations of mycorrhizal plants. Biomass increases due to N were larger when the plants were not fertilized with additional P. P fertilization reduced the colonized root length and biomass production of mycorrhizal plants. The base treatment (Ca+Mg) did not significantly affect biomass production but increased the colonized root length. These results stress the importance of evaluating the VAM rice symbiosis under various fertilization regimes. The results of this study suggest that pretransplant mycorrhizal rice plants may have a potential for better field establishment than non-mycorrhizal plants.
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  • 29
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhizae ; Fluorescent Pseudomonas spp. ; Oryza sativa ; Pretransplant ; Nursery ; Rhizosphere interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study examined the response of rice (Oryza sativa L.) plants at the pretransplant/nursery stage to inoculation with vesicular-arbuscular mycorrhizal (VAM) fungi and fluorescent Pseudomonas spp., singly or in combination. The VAM fungi and fluorescent Pseudomonas spp. were isolated from the rhizosphere of rice plants. In the plants grown in soil inoculated with fluorescent Pseudomonas spp. alone, I found increases in shoot growth, and in root length and fine roots, and decreases in root growth, and P and N concentrations. In contrast, in the plants colonized by VAM fungi alone, the results were the reverse of those of the pseudomonad treatment. Dual inoculation of soil with VAM fungi and fluorescent Pseudomonas spp. yielded plants with the highest biomass and nutrient acquisition. In contrast, the plants of the control treatment had the lowest biomass and nutrient levels. The dual-inoculated plants had intermediate root and specific root lengths. The precentages of mycorrhizal colonization and colonized root lengths were significantly lower in the dual-inoculated treatment than the VAM fungal treatment. Inoculation of plants with fluorescent Pseudomonas spp. suppressed VAM fungal colonization and apparently reduced photosynthate loss to the mycorrhizal associates, which led to greater biomass and nutrient levels in dual-inoculated plants compared with plants inoculated with VAM fungi alone. Dual inoculation of seedlings with fluorescent Pseudomonas spp. and VAM fungi may be preferable to inoculation with VAM alone and may contribute to the successful establishment of these plants in the field.
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  • 30
    ISSN: 1432-0789
    Keywords: Mixed cropping ; Biological efficiency ; Nitrogen uptake ; Cowpea ; Upland rice ; Oryza sativa ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sources of N used by cropped and intercropped cowpeas and rice were determined by the 15N isotope-dilution technique. The biological efficiency of intercropping cowpeas and rice was assessed by calculating the land equivalent ratio of dry matter yield, total N, and uptake of N. A reduced N uptake by both cowpeas and rice during mixed cropping was attributed to mutual competition, with both crops competing effectively for the scarce environmental resources. The lack of a significant difference in the uptake of fertilizer and soil N by mixed-crop rice and cowpeas is an indication that the soil N was sufficient and that the mixed cropping did not create any imbalance in soil and fertilizer N uptake. The land equivalent ratio ranged between 120% and 180% for shoot dry matter and total N, showing that biological efficiency was increased by intercropping cowpeas with rice. The proportion of N derived from the atmosphere by mono- and intercropped cowpeas was not significantly different, showing that the potential of cowpeas to fix N2 was independent of the cropping system, since the rice did not stimulate the cowpeas to fix more N2.
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  • 31
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    Biology and fertility of soils 14 (1992), S. 1-4 
    ISSN: 1432-0789
    Keywords: Isouron ; Rice seedling ; Uptake ; Translocation ; Metabolism ; Broadleaf herbicide ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Isouron, a pre-emergence herbicide, inhibited the growth of rice seedlings in a nutrient solution; the critical concentration was about 0.10 mg l-1. Isotope studies showed that isouron, added to the nutrient solution, was observed by the rice roots and translocated rapidly to the shoot. In rice seedlings, isouron was converted to six known metabolites, 3-(5-(1-dimethyl-2-hydroxy-ethy)-3-isoxazolyl]urea, 3-(5-tert-butyl-3-isoxazolyl)-1-methylurea, 3-(5-tert-butyl-3-isoxazolyl)ure, 3-amino-5-tert-butylisoxazole, 3-[5-(1,1-dimethyl-2-hydroxyethyl)-3-isoxazolyl]-1-dimethylurea, and 3-[5-(1,1-dimethyl-2-hydroxyethyl)-3-isoxazolyl]-1-methylurea, and two unknown metabolites.
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  • 32
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    Euphytica 63 (1992), S. 115-123 
    ISSN: 1573-5060
    Keywords: environmental influence ; Oryza sativa ; Pyricularia grisea ; P. oryzae ; rice ; rice blast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Blast is one of the most serious diseases of rice worldwide. The pathogen,Pyricularia grisea, can infect nearly all parts of the shoot and is commonly found on the leaf blade and the panicle neck node. Host resistance is the most desirable means of managing blast, especially in developing countries. Rice cultivars with durable blast resistance have been recognized in several production systems. The durable resistance of these cultivars is associated with polygenic partial resistance that shows no evidence of race specificity. This partial resistance is expressed as fewer and smaller lesions on the leaf blade but latent period does not appear to be an important component. Partial resistance to leaf blast is positively correlated with partial resistance to panicle blast, although some cultivars have been found showing leaf-blast susceptibility and panicle-blast resistance. A diverse set of environmental factors can influence the expression of partial resistance, including temperature, duration of leaf-wetness, nitrogen fertilization, soil type, and water deficit. Because of the great diversity of rice-growing environments, resistance that proves durable in one system may or may not prove useful in another. In highly blast-conducive environments, other means of disease management must be applied to assist host-plant resistance.
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  • 33
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    Euphytica 64 (1992), S. 161-165 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; grain weight ; grain density ; genetic effects ; heritability ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Increasing grain weight is one means of increasing rice (Oryza sativa L.) grain yields. Selection for increased density of filled grains may offer an approach to increasing rice grain weight. Before rice breeding programs can begin effectively selecting for higher grain density, the nature and amount of the genetic variation present must be evaluated. A Design II mating plant with two sets was constructed using 16 parents. The 16 parents were representative of cultivars and elite breeding material available to breeders of long-grain rice in the southern U.S. The parents and 32 F1 hybrids were evaluated in 1990 at two Arkansas locations: Stuttgart and Marianna. Additive variation was essentially zero for grain density as indicated by nonsignificant general combining ability (GCA) and specific combining ability (SCA) mean squares and a narrowsense heritability estimate −0.04 (±0.07). Increasing rice grain weight through increased grain density would not be feasible in U.S. southern long-grain rice unless new germplasm with higher filled grain densities is introduced. Genetic variation for grain weight was predominately additive and complemented with additive x additive epistatic variation, indicating that selection for increased grain weight could be practiced in segregating generations. The small proportion of epistatic variation to additive variation would not justify delaying selection until epistatic combinations are fixed in the homozygous line. Midparent grain weight means would serve as an adequate indicator of progeny performance for cross appraisal.
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  • 34
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    Euphytica 64 (1992), S. 143-148 
    ISSN: 1573-5060
    Keywords: blast ; Magnaporthe grisea ; Pyricularia oryzae ; Oryza sativa ; race-non-specific resistance ; face-specific resistance ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Six rice genotypes, differing in partial resistance, were exposed to three isolates of the blast pathogen. Of the variance due to host and pathogen genotypes, 39% was due to host genotype effects, 60% was due to isolate effects, and only 1% was due to host genotype × isolate interactions. Although small, this interaction variance was highly significant and mainly due to the IR50 × W6-1 and IR37704 × JMB8401-1 combinations. Although behaving largely as race-non-specific (large main effects only), the partial resistance cannot be classified as race-non-specific. The results suggest that minor genes for partial resistance operate in a gene for gene relationship with minor genes in the pathogen.
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  • 35
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    Euphytica 63 (1992), S. 23-31 
    ISSN: 1573-5060
    Keywords: genetic resources ; gene bank ; pearl millet ; Pennisetum glaucum ; potato ; Solanum tuberosum ; rice ; Oryza sativa ; cotton ; Gossypium spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic variation in crop species and their wild relatives holds the key to the successful breeding of improved crop cultivars with durable resistance to disease. The importance of the conservation, characterization and utilization of plant genetic resources nationally and internationally has been recognised, though much remains to be done. Gene banks have now been established in many countries and at most of the international crop research centres. Cell and tissue culture techniques and biotechnological aids have done much to ensure the creation and safe transfer of healthy germplasm around the world. Multidisciplinary, international research and collaboration are essential to the successful breeding of improved disease resistant cultivars. Examples are given of the effective use of genetic resources in breeding disease resistant cultivars of a number of crops, including cotton, rice, potatoes and pearl millet.
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  • 36
    ISSN: 1573-5060
    Keywords: α-amylase enzyme activity ; α-amylase mRNA levels ; cold temperature ; differential gene expression ; Oryza sativa ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This report examines the relationship between seedling vigor, α-amylase enzyme activity and α-amylase mRNA accumulation in ten varieties of rice (Oryza sativa L.) grown at two temperatures (15°C and 30°C). A significant, positive correlation was observed between seedling vigor, α-amylase enzyme activity, and the accumulation of mRNA from one rice α-amylase gene (RAmy1A) at both temperature regimens. The results of this study support previous experiments which have correlated α-amylase enzyme activity to seedling vigor. We have extended this correlation to the expression of one of ten genes that comprise the rice α-amylase multigene family. These results suggest that the expression of α-amylase gene RAmy1A is an important, and possibly rate-limiting factor in determining seedling vigor in rice.
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  • 37
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    Euphytica 63 (1992), S. 271-279 
    ISSN: 1573-5060
    Keywords: leaf blast ; Magnaporthe grisea ; Oryza sativa ; partial resistance ; Pyricularia oryzae ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten rice genotypes were inoculated with a virulent isolate of Magnaporthe grisea to study the effect of leaf age on components of partial resistance, and evaluate associations between these components. The number of sporulating lesions per cm2 leaf declined with increase of leaf age in all genotypes. The number of lesions per cm2 leaf area in one week old leaves was about 25% that of the number in very young leaves in the susceptible cultivar CO39, but less than 2% in the more resistant cultivars IR36, IR60 and IR62. Large differences between genotypes were found for the number of sporulating lesions that developed, and this factor was closely related to the period that leaves remained susceptible after appearance. The number of lesions in the most susceptible cultivar CO39 was about 7 times that in the cultivars IR60 and IR64. Differences between genotypes were also found for lesion size. The effect of aging on average lesion size was less pronounced than on lesion density. Lesion size and lesion density were positively correlated, but a rapid decline of density was not necessarily accompanied by a rapid decline of size. No apparent differences between genotypes were observed for latent period. Genotypes with leaves that became highly resistant soon after appearance expressed higher levels of partial resistance in the field.
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  • 38
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    Euphytica 64 (1992), S. 71-80 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; wide crosses ; hybrid sterility ; wide compatibility genes ; genetic analysis ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wide compatible varieties (WCVs) show normal spikelet fertility in crosses with Indica and Japonica rice varieties. Crosses of Indica and Japonica varieties frequently show high spikelet sterility which prevents exploitation of heterosis for grain yield. We screened 41 rice varieties for the wide compatibility trait by crossing each with three Indica and three Japonica testers. Varieties giving fertile F1 hybrids with both groups of testers were classified as WCVs. Seven varieties viz., BPI-76 (Indica); N 22; Lambayeque-1 and Dular (Aus); Moroberekan, Palawan and Fossa HV (Japonicas), were identified as WCVs. The frequency of WCVs was higher among Aus and Japonicas. The wide compatibility trait in varieties: Dular and Moroberekan was controlled by a single dominant gene linked with the Est-2 and Amp-3 loci (mean recombination 32.0%). Est-2 and Amp-3 showed complete linkage. Pgi-2 was found to be linked with Est-2 and Amp-3 (mean recombination 16.1%). Est-2 and Amp-3, showed a tighter linkage with C + (mean recombination 4.1%). Pgi-2 showed a lower linkage with C + (mean recombination 17.3%). The recombination values between the WC gene in Dular and C + was much higher than those reported in Japan for the WC gene (S5 n) from Ketan Nangka. It is possible that the WC gene from Dular is different from that in Ketan Nangka. Linkage intensities with the WC gene were not strong enough to be of use for indirect selection for the wide compatibility trait. A search for a more closely linked isozyme or DNA marker was proposed.
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  • 39
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; indica-japonica interracial hybrids ; haploid regeneration ; fertility restoration ; doubled haploid line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Response of anthers in in vitro culture was examined in the indica-japonica hybrids of rice (Oryza sativa L.). Significant genotypic differences were observed for callus induction and regeneration among the different interracial hybrids of indica-japonica races. Induction frequency of haploids ranged from 57.7 to 72.9 per cent and doubled haploid androgenic lines ranged from 27.1 to 42.3 per cent in the anther culture of the different hybrids. The indica-japonica hybrids recorded partial pollen grain and spikelet fertility in F1 (29.9 to 41.5% and 19.4 to 48.7% respectively) as well as in F2 (42.7 to 50.6% and 37.1 to 54.4% respectively). In contrast, the androgenic doubled haploid lines recorded significant increase and the pollen grain and spikelet fertility was 76.3 and 78.6 per cent respecitively. The results suggested that the sterility barriers for realising genetic recombinants and fixation of fertile homozygous lines in indica-japonica hybridization programme could be overcome through F1 anther culture technique.
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  • 40
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    Euphytica 64 (1992), S. 173-182 
    ISSN: 1573-5060
    Keywords: Magnaporthe grisea ; Oryza sativa ; Pyricularia oryzae ; yield components ; yield loss ; partial resistance ; rice neck blast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Neck nodes of eight rice genotypes were inoculated with a virulent isolate of the blast pathogen at four slightly different, increasingly older stages of panicle development shortly after flowering. Resistance to infection as well as resistance to growth of the pathogen after infection was assessed. Significant differences between genotypes were found for both components of resistance. Resistance to growth of the pathogen sharply increased with aging of the neck node as was indicated by a large reduction of the length of the lesions on the culm, but resistance to infection did not change much. The lesion length was closely related to yield loss. A small delay in time of infection can lead to a large decrease in yield reduction. Meaningful comparison of partial resistance to neck blast between genotypes therefore requires infection at an identical stage of panicle development. In the field, where panicles are in different stages of development, selection of genotypes that do show the symptoms of neck blast together with relatively little yield loss in infected panicles is recommended.
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  • 41
    ISSN: 1573-5060
    Keywords: leaf blast ; Oryza sativa ; photosynthesis ; Pyricularia oryzae ; rice ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of an inoculation with Pyricularia oryzae (isolate P06-6) on net leaf photosynthetic rate of rice (Oryza sativa) was studied with four cultivars. Measurements were taken on the sixth leaf of the main culm of plants in the early tillering stage. On cultivars CO39, IR50 and IR64 a susceptible infection type developed, but a clear difference in relative infection efficiency of the cultivars was observed. The highest number of lesions developed on leaves of CO39, whereas the lowest number was found on leaves of IR64. For all three cultivars the effect of a single lesion on the reduction in net leaf photosynthetic rate was found to be equal to a reduction in leaf area of three times the area occupied by the visible lesion. On IR68, a cultivar with complete resistance, brown specks of pinpoint size appeared without any effect on net leaf photosynthetic rate.
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  • 42
    ISSN: 1573-5036
    Keywords: biological nitrogen fixation ; denitrification ; fallow ; flooded soil ; leaching ; legume ; nitrate ; nitrogen balance ; nitrogen loss ; Oryza sativa ; rice ; weeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lowlands comprise 87% of the 145 M ha of world rice area. Lowland rice-based cropping systems are characterized by soil flooding during most of the rice growing season. Rainfall distribution, availability of irrigation water and prevailing temperatures determine when rice or other crops are grown. Nitrogen is the most required nutrient in lowland rice-based cropping systems. Reducing fertilizer N use in these cropping systems, while maintaining or enhancing crop output, is desirable from both environmental and economic perspectives. This may be possible by producing N on the land through legume biological nitrogen fixation (BNF), minimizing soil N losses, and by improved recycling of N through plant residues. At the end of a flooded rice crop, organic- and NH4-N dominate in the soil, with negligible amounts of NO3. Subsequent drying of the soil favors aerobic N transformations. Organic N mineralizes to NH4, which is rapidly nitrified into NO3. As a result, NO3 accumulates in soil during the aerobic phase. Recent evidence indicates that large amounts of accumulated soil NO3 may be lost from rice lowlands upon the flooding of aerobic soil for rice production. Plant uptake during the aerobic phase can conserve soil NO3 from potential loss. Legumes grown during the aerobic phase additionally capture atmospheric N through BNF. The length of the nonflooded season, water availability, soil properties, and prevailing temperatures determine when and where legumes are, or can be, grown. The amount of N derived by legumes through BNF depends on the interaction of microbial, plant, and environmental determinants. Suitable legumes for lowland rice soils are those that can deplete soil NO3 while deriving large amounts of N through BNF. Reducing soil N supply to the legume by suitable soil and crop management can increase BNF. Much of the N in legume biomass might be removed from the land in an economic crop produce. As biomass is removed, the likelihood of obtaining a positive soil N balance diminishes. Nonetheless, use of legumes rather than non-legumes is likely to contribute higher quantities of N to a subsequent rice crop. A whole-system approach to N management will be necessary to capture and effectively use soil and atmospheric sources of N in the lowland rice ecosystem.
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  • 43
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    Biology and fertility of soils 11 (1991), S. 97-100 
    ISSN: 1432-0789
    Keywords: Added N interaction ; Fertilizer N ; 15N ; Organic amendment ; Oryza sativa ; Wetland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A pot experiment was conducted to study the availability of soil and fertilizer N to wetland rice as influenced by wheat straw amendment (organic amendment) and to establish the relative significance of the two sources in affecting crop yield. Straw was incorporated in soil at 0.1, 0.2, and 0.3% before transplanting rice. Inorganic N as 15N-ammonium sulphate was applied at 30, 60, and 90 μg g-1 soil either alone or together with wheat straw in different combinations. After harvesting the rice, the plant and soil samples were analyzed for total N and 15N. Straw incorporation significantly decreased the dry matter and N yield of rice, the decrease being greater with higher rates of straw. The reduction in crop yield following the straw incorporation was attributed mainly to a decrease in the uptake of soil N rather than fertilizer N. The harmful effects of organic matter amendment were mitigated by higher levels of mineral N addition. The uptake of applied N increased and its losses decreased due to the straw incorporation. Mineral N applied alone or together with organic amendment substantially increased the uptake of unlabelled soil N. The increase was attributed to a real added N interaction.
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  • 44
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    Biology and fertility of soils 12 (1991), S. 213-216 
    ISSN: 1432-0789
    Keywords: Soil management problems ; Multiply cropped paddy fields ; Mineral fertilizer ; Organic manure ; Sustainable agriculture ; Oryza sativa ; Trificum sativa ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Multiple cropping of paddy fields has now been widely adopted in East China, in order to increase annual grain yields. The management practices recommended for these multiple cropped fields include the use of mineral fertilizers in combination with organic manure, to sustain a better soil nutrient balance, the adoption of rice-straw manuring, and use of zero or minimum tillage. This paper presents the results of a series of longterm experiments in multiple cropping and discusses the problems associated with these techniques.
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  • 45
    ISSN: 1573-5060
    Keywords: chemical mutagenesis ; male sterility ; Oryza sativa ; pollen ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty-six male sterile plants grown in the field were recovered in the M7 generation from ethyl methane sulfonate-treated material of the rice cultivar M-201. Fertility increased five-fold when ratooned plants from the field were grown in a growth chamber with a 12 hour daylength. Crosses between mutant and normal fertile cultivars produced fertile F1 plants. Female fertility was normal as judged by percent seed set from unbagged panicles of parental and recombinant lines. Transgressive segregation for fertility was observed for all crosses in the F2 and F3 generations. Five of 37 F3 male sterile plants showed moderate levels of seed fertility under winter greenhouse conditions and reduced seed set when transplanted to summer field plots. Fertility data from reciprocal crosses suggested cytoplasmic factors had little or no effect on levels of male sterility in the mutant lines. Chi-squared analyses of F2 and F3 generation results indicated male sterility of the mutants is conditioned by two nuclear genes with epistatic effects.
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  • 46
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; Xanthomonas campestris pv. oryzae ; bacterial blight ; ghost gene ; quantitative resistance ; residual resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary F2 plants of five, and F3 plants of three, crosses between genotypes carrying the race-specific resistance gene Xa-4 and genotypes not carrying this gene were inoculated with two isolates of Xanthomonas campestris pv. oryzae. Half the tillers of each plant received isolate PX061, avirulent on the Xa-4 gene, the other half of the tillers received isolate PX099, virulent for the Xa-4 gene. The F2 and F3 populations segregated for a single dominant resistance gene, Xa-4. The parental, F2 and F3 genotypes not carrying Xa-4 had mean lesion lengths between 28 and 29 cm for both isolates. The Xa-4 carrying parents showed a mean lesion length of 2.7 cm with the avirulent isolate and of 12.4 cm with the virulent isolate. The Xa-4 carrying F2 and F3 genotypes had mean lesion lengths of 5.2 and 20.1 cm for the two isolates, respectively. These observations strongly indicate that the Xa-4 gene, carried by the rice genotypes studied (IR28, Cisadane and BR51-282-8), had a considerable residual effect when exposed to virulent isolate PXO99.
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  • 47
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    Euphytica 56 (1991), S. 43-46 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; genetic diversity ; germplasm ; Taiwan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The pedigrees of 99 Japonica rice (Oryza sativa L.) varieties released between 1940 and 1987 were traced back to 65 ancestors, of which 44 were plant introductions from Japan and only 11 from Taiwan. Japanese introductions collectively contributed more than 85% of the parentage of the varieties. Although more ancestors were integrated into recent breeding programs, as few as 10 ancestors comprised 66% of the genetic background. Extensive use of superior genotypes from the same sources explained why the integration of new germplasm did not result in the diversification of the released varieties. The 10 most important ancestral contributors of the 99 varieties were from Japan except ‘Oloan-chu’ from Taiwan. Two introductions, ‘Sinriki’ and ‘Kameji’, had the highest mean relative genetic contributions of the genes with 21.3% and 16.7%, respectively. Shinriki was the most frequently used ancestor and occurred in 83 of the 99 pedigrees. These results clearly revealed the narrow genetic base in current Japonica rice varieties of Taiwan.
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  • 48
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; male fertility restoration ; genetics ; cytoplasmic male sterility ; epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetics of fertility restoration To avoid tedious repetitions we refer to male fertility and male sterility as fertility and sterility, respectively. in six varieties and breeding lines of rice was studied in ‘Wild Abortive’ cytoplasmic genetic male sterility system using cytoplasmic male sterile lines V 20 A and IR 54752 A. Fertility evaluation of the plants in F2 and testcross populations of the crosses of V 20 A with PR 103, PR 106 and PAU 502-94-1, and IR 54752 A with PAU 1124-36-1 and PAU 1126-1-1 revealed that fertility restoration in PR 103, PR 106, PAU 502-94-1, PAU 1124-36-1 and PAU 1126-1-1 was controlled by two independently segregating dominant genes. The two genes appeared to have additive effects; one of them being stronger than the other in imparting fertility restoration. Data on spikelet fertility of the plants in F2 and testcross populations of V 20 A/UPR 82-1-1 cross showed that fertility restoration in UPR 82-1-1 was controlled by two independently segregating dominant genes which exhibited recessive epistatic interaction.
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  • 49
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    Euphytica 54 (1991), S. 147-154 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; germplasm ; genetic resources ; conservation ; rice pests ; rice pathogens ; core collections ; search strategies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using the evaluation database on the world collection of rice, Oryza sativa, conserved at the International Rice Research Institute, different sampling strategies for choosing germplasm were compared. Random, stratified, sequential and analysed sets of germplasm were chosen and the frequency of finding resistance to different rice pests, the brown planthopper, green leafhopper and whitebacked planthopper, and diseases, bacterial blight and blast were compared. The frequency of the geographically restricted javanica race of rice was also compared in the different germplasm sets. The results indicate that where no prior information is available to choose germplasm for evaluation, for the same sample number, germplasm representing broad genetic diversity are preferable to other sampling strategies.
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  • 50
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    Euphytica 58 (1991), S. 241-244 
    ISSN: 1573-5060
    Keywords: Cold tolerance ; cytoplasmic-genetic male sterile line ; cytoplasmic inheritance ; hybrid rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The cytoplasmic-genetic male sterile line, ‘Krishna-A’ with ‘Kalinga-I’ cytoplasm was developed in rice through repeated backcrossing the completely pollen sterile F1 of the cross ‘Kalinga-I/Krishna’ with the recurrent male parent cv. Krishna. The germination percentage and root-shoot length in ‘Krishna-A’ at 12°C and 9°C indicated a higher degree of tolerance to low temperature than the cold tolerant female parent ‘Kalinga-I’. The high yielding cold tolerant variety ‘Kalinga-I’ was developed from a cross involving the cold tolerant variety ‘Dunghansali’ and the high yielding variety ‘IR 8’. It is inferred that tolerance to low temperature during germination in ‘Krishna-A’ was inherited from the cytoplasm of cv. Dunghansali through ‘Kalinga-I’ besides male sterility. The cytoplasmic control of cold tolerance is reported here for the first time in rice literature. The male sterile line ‘Krishna-A’ because of its tolerance to low temperature would be suitable for the development of hybrid rice especially for areas where low temperature is a problem during germination.
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  • 51
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; Xanthomonas campestris pv. oryzae ; bacterial blight ; quantitative resistance ; polygenic resistance ; transgressive segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four cultivars moderately resistant to Philippine isolates of Xanthomonas campestris pv. oryzae races 2, 3, 5 and 6 and highly resistant to race 1 were crossed with two susceptible cultivars and with each other. The F1 populations were as or more susceptible than the least resistant parent cultivar when assessed for lesion length (cm) by clip-inoculating booting plants with two race 2 and one race 6 isolates of X.c. oryzae. The F2 populations showed continuous distributions when assessed with the race 6 isolate PX099, although populations from crosses between moderately resistant cultivars were positively skewed. Mean broad-sense heritability in the F2 was 0.50. Selection for varying levels of resistance was carried out in the F2 and F3 generations. F3 lines selected from the F2 population modes had variances and ranges equal to those selected from the F2 population extremes and larger than the variances of the parent cultivars. Line selection in the F3 generation was more effective than plant selection in the F3 and in the F2. Realized h2 was 0.39 for line selection in the F3 but only 0.24 for plant selection. A number of lines more resistant than both parents were recovered in crosses between moderately resistant cultivars. Lines more susceptible than both parents were also recovered in crosses between moderately resistant cultivars, but few of these lines were as susceptible as the susceptible cultivars. This indicates that the moderately resistant cultivars had some resistance factor(s) in common. All test cultivars, including the susceptible cultivars, carry few to several factors for quantitative resistance. A model based on nine minor resistance factors is proposed to explain the pattern of transgression found in crosses between the six cultivars.
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  • 52
    ISSN: 1573-5060
    Keywords: Xanthomonas campestris pv. oryzae ; bacterial blight ; rice ; Oryza sativa ; clip inoculation ; spray inoculation ; quantitative resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Lesion size and lesion number were measured on cultivars of rice inoculated by clipping or spraying with virulent isolates of Xanthomonas campestris pv. oryzae. Mean percentage diseased leaf area (%DLA) gave a similar ranking for the two inoculation methods but differences in lesion size among cultivars were much more evident after clip than after spray inoculation. Correlation between the methods was high (r=0.82**), but some cultivars responded differently with the two techniques. Cultivars which had low scores following spray inoculation showed low disease progress during the first nine weeks after transplanting into a screen-house experiment. Assessment after clip inoculation measures resistance due to spread of bacteria within the leaf xylem system, an important component of quantitative resistance. Assessment after spray inoculation measures all resistance, including resistance to entrance of bacteria into the leaf. In order to select rice entries with improved quantitative resistance to X. c. oryzae based on both components, a screening based on lesion length after clip inoculation, followed by a test for lesion number after spray inoculation, is advised.
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  • 53
    ISSN: 1573-5036
    Keywords: acetylene reduction ; bacteria ; greenhouse ; N2-fixation ; Oryza sativa ; rhizoplane ; scanning electron microscopy ; rhizosphere ; top dry weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With an autotrophic, N-free medium, Xanthobacter populations were isolated from the roots of wetland rice grown under field conditions. Xanthobacter populations ranged from 3.2×104 to 5.1×105 colony-forming units (cfu) g-1 of root and averaged 47-fold higher on the root or rhizoplane than in the neighbouring nonrhizosphere. Characterization studies indicated dissimilarities in carbon utilization and motility among the isolated Xanthobacter strains and other recognized Xanthobacter species. Under gnotobiotic conditions, the population of one isolate, Xanthobacter sp. JW-KR1, increased from 105 to 107 cfu plant-1 1 d after inoculation when a rice plant was present, but declined to numbers below the limit of detection (〈104 cfu assembly-1) after 3 d in the absence of a plant. Scanning electron microscopy revealed Xanthobacter as pleomorphic forms on the rhizoplane. To assess the effect of Xanthobacter on plant growth, rice plants were grown under greenhouse conditions in plant assemblies containing sand and half-strength Hoagland's nutrient solution with and without nitrogen. Plants were either inoculated with 105 cfu Xanthobacter g-1 of sand or left uninoculated. After 40 d, plants without nitrogen showed no significant differences in top or root dry weight, plant height, root length, or number of tillers or leaves, whether the plants were inoculated or uninoculated. However, when nitrogen was added, inoculated plants had a significantly larger top dry weight (15%) and number of leaves (19%) than uninoculated plants. Under conditions of added and no added nitrogen, acetylene reduction assays showed Xanthobacter sp. JW-KR1 produced 〈0.1 (below detection limit) and 7 nmol C2H4 plant-1 h-1, respectively. Under the conditions studied, the results suggest that both Xanthobacter and wetland rice derive some benefits from their association.
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  • 54
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    Biology and fertility of soils 9 (1990), S. 68-70 
    ISSN: 1432-0789
    Keywords: Leucaena leucocephala ; Green manure ; N Uptake ; Yield ; Wetland rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field studies were conducted over two seasons to examine the effect of Leucaena leucocephala as a green manure on the N uptake and yield of rice grown under lowland conditions. The treatments were 0, 4, 8, and 12t Leucaena ha-1 with 0,44, and 88 kg N ha-1 as urea in a factorial combination. N uptake was evaluated at maximum tillering, panicle initiation, and harvest. The incorporation of Leucaena increased N uptake throughout the vegetative period in both seasons, irrespective of the mineral-N level. At all levels of N, the grain yield increased significantly following the incorporation of Leucaena, and in both seasons the Leucaena treatment of 8 t ha-1 was almost as effective as the highest mineral-N application.
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  • 55
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    Euphytica 49 (1990), S. 203-207 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Sorghum bicolor ; Triticum aestivum ; C3–C4 characters ; growth analysis ; rice × sorghum ; rice × wheat hybrids ; root studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Some photosynthetic characters as-leaf anatomy, leaf photosynthetic rate and CO2 compensation pointdistinguishing C3 and C4 plants and physiological characters as leaf area ratio, nitrogen content, leaf stem ratio and total shoot-to-root and deep root-to-shoot ratios have been studied in rice × sorghum and rice × wheat hybrids. Rice × sorghum 1. has lower values of photosynthetic rate, leaf nitrogen, total root and deep root-to-shoot ratio and CO2 compensation point as of rice parent where as, rice × sorghym 2. is superior in all these characters. Both hybrids lack kranz anatomy. Though both rice × sorghum hybrids show characters of C3 rice plant but rice × sorghum 2. has improved drought tolerance and leaf characters in relation to yield. Rice × wheat hybrid have higher assimilatory area and higher total root-to-shoot ratio. Grains of rice × wheat hybrids are identical to rice grain. However, as grains of rice × wheat hybrid does not contain seed coat, it could be exploited as novel rice germplasm after improvement.
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  • 56
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    Euphytica 51 (1990), S. 87-93 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; heterosis ; heterobeltiosis ; standard heterosis ; heterosis x environment interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten CMS (A) lines and their maintainers (B) and seven restorers (R) were used to make 70 crosses (A/R) and 70 reciprocals (R/B) following line x tester design. The 140 crosses and 17 parents (10 B + 7 R lines) were evaluated in six environments created by three nitrogen fertility levels (0, 60, 120 kg N/ha) and two seasons wet (WS) and dry (DS). Hybrids (both A/R and R/B) were superior to their parents in yield. Hybrids flowered earlier and were taller than the parents. Substantial heterosis, heterobeltiosis and standard heterosis were observed in different environments. Extent of heterosis was higher in WS (stress environment) than in DS (favorable environment). Twenty four hybrids outyielded the standard check (IR46) significantly. Most of them were derived from crosses with parental lines: IR54752A/B, IR58057A/B, IR46 and IR54. For days to flowering, the overall mean heterosis, heterobeltiosis and standard heterosis were all negative values. Heterosis for plant height did not change the plant type of hybrids from semi-dwarf to tall because the parents possessed same dwarfing gene. With proper choice of parents hybrids possessing higher yield potential, early maturity and semi-dwarf plant type can be developed for the tropics.
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  • 57
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; cytoplasmic-genetic male sterile line ; Indica cytoplasm ; Japonica rice ; reciprocal differences in pollen sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary From 28 Indica-Japonica crosses, two Indica cultivars, V.20B and Sattari were identified to possess male sterile cytoplasm with fertility restoring genes. It was possible to develop a new Japonica cytoplasmic genetic male sterile line (Zhunghua-1) on Indica male sterile cytoplasm (V 20B) by repeated backcrossing the complete pollen sterile plants of V 20B x Zhunghua-1 to the recurring male parent, Zhunghua-1. The study indicated that it would be possible to develop male sterile lines rom indica-japonica crosses only when there is sufficient amount of reciprocal differences with respect to pollen sterility. Further, it was inferred that it would be easier to develop Japonica male sterile lines on Indica cytoplasm than developing Indica male sterile line with japonica cytoplasm.
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  • 58
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    Euphytica 46 (1990), S. 157-159 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; aroma ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inheritance of an aroma was worked out in crosses between ‘Brimful’ from Nepal as an aromatic rice and leading Japanese varieties ‘Koshihikari’ and ‘Nipponbare’ as non-aromatic ones. The F2 pattern of segregation for aroma to non-aroma was 3:13 indicating one dose gene for aroma and one dose inhibitor gene in two crosses. This ratio was confirmed by genetic behavior of F3 populations.
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  • 59
    ISSN: 1573-5060
    Keywords: ecotype ; indica ; japonica ; lowland rice ; Oryza sativa ; upland rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rice cultivars (Oryza sativa L.) belonging to five ecotypes (aus, aman, boro, bulu and tjereh) and to two groups of Japanese rice (lowland and upland) are examined with respect to KClO3 resistance, phenol reaction and apiculus hair length. These characters have been used as available criteria to classify rice into two types indica and japonica, for the last thirty years. The findings of this study are that the aman, boro and tjereh ecotypes should be classified as typical indica; and that the Japanese lowland rice cultivars are mainly typical japonica. Some of the aus, bulu and Japanese upland rice cultivars differ from typical indica and typical japonica, so the respective terms aus type, bulu type and J.u.r. type, are proposed. Aman, boro tjereh and Japanese lowland rice are cultivated in lowland. Some of the aus, bulu and Japanese upland rice cultivars have the characteristics of upland rice. In general, lowland rice cultivars can be clearly classified into indica or japonica, while upland cultivars cannot.
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  • 60
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    Euphytica 46 (1990), S. 195-202 
    ISSN: 1573-5060
    Keywords: Manihot esculenta ; cassava ; Oryza sativa ; rice ; selection ; competition ; yield ; biomass yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Relative importance of harvest index (I) and total biomass yield (B) to economic yield (Y) was assessed in several food crops at different levels of environmental productivity. Importance of B is generally higher in low than high yielding environments, while that of I is higher in high than low yielding environments. In some crops B is important throughout different yield levels while in others I is important even in low yielding environments. Past efforts by anonymous farmers have consummated a good part of genetic improvement of crop yields through improvement in B. Many venerable land cultivars of grain crops, adapted to unimproved, limited-input cultural conditions, evolved through this process. The same process may not have thoroughly exhausted the yield improvement opportunity through improving I. Success in yield improvement by modern breeding has been limited mainly to high-input cultural conditions characterized by higher soil fertility and irrigation mainly through improvement in I. Varietal improvement possibility for less productive environments is discussed.
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  • 61
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; Sogatella furcifera ; whitebacked planthopper ; insect resistance ; allelic relationship ; inheritance of resistance ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetics of resistance to whitebacked planthopper, Sogatella furcifera (Horvath) in ten resistant cultivars was studied. The reactions of the F1, F2 and F3 populations of resistant varieties with Taichung Native 1, a suspectible check, showed that WBPH resistance is monogenic in nature and governed by dominant gene(s) in Ptb 19 and IET 6288 and recessive gene in eight cultivars viz. ARC 5838, ARC 6579, ARC 6624, ARC 10464, ACR 11321, ARC 11320, Balamawee and IR 2415-90-4-3. Allelic relationship of resistance gene(s) in the test cultivars revealed recessive gene in IR 2415-90-4-3, ARC 5838 and ARC 11324 to be allelic but it was non allelic to the resistance gene in ARC 6624. Cultivars ARC 6579, ARC 11321 and Balamawee have identical gene among themselves but their relationship with IR 2415-90-4-3, ARC 5838, ARC 11324 and ARC 6624 is unknown. The recessive gene in ARC 10464 is non-identical to all other cultivars having the recessive gene except ARC 6624 with which its relationship needs further investigation.
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  • 62
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    Euphytica 48 (1990), S. 91-95 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; ATPase ; succinic dehydrogenase ; heterosis ; correlation ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Activities of ATPase and succinic dehydrogenase were assessed in three F1 hybrids of rice and their parental lines during boot leaf stage, flowering, on 10th and 20th days after flowering. ATPase activity showed increase at the flowering and on 20th day after flowering. Succinic dehydrogenase activity continued to rise till 10th day after flowering and declined on 20th day after flowering. Hybrids with high yield were generally endowed with more positive heterosis for these two enzymes. Correlation coefficient of grain yield per panicle was significantly positive with ATPase activity at all the stages studied. Heterosis for ATPase activity might serve a reliable criterion for the selection of efficient F1 combinations.
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  • 63
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; clonal propagation ; cytokinins ; shoot proliferation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Shoot base segments have been explanted from seedlings of rice (Oryza sativa L. subsp. Japonica, cv. Arborio) and grown on agar-solidified MS medium supplemented with different concentrations of four cytokinins: kinetin, BAP, 2iP and zeatin. After one month, segments were explanted from proliferated shoots and subcultured on their respective media. BAP was by far the most effective in inducing shoot proliferation. Highest rates were achieved at the higher concentration used: 5 mg 1−1. Shoot base segments were subcultured fifteen times consecutively on seven different concentrations of BAP. Shoots grown in the presence of 5 mg 1−1 of BAP proliferated an average of 12 normal shoots for each base segment throughout the fifteen subcultures. The shoots rooted easily on hormone-free medium. The technique does not require any particular skill, it is very effective and, therefore, can be suggested as suitable for clonal propagation of rice.
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  • 64
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; drought resistance ; screening methods ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Although many selection indices have been used to screen rices (Oryza sativa L.) for drought resistance, there has been little comparison of the relative merits of these indices. Research was conducted to compare drought resistance as estimated from grain yields, canopy-temperature-based stress indices, visual scoring, and uprooting force for 30 rice genotypes grown in the field with a puddled Maahas clay (Typic Tropaquept) and to evaluate traits related to drought resistance from nonstressed plants grown in the field and in aeroponic culture. Water deficit was imposed in the field by withholding irrigation from 45 to 75 days after transplanting compared to a continuously flooded control. Grain yields in the stress treatment were most strongly correlated with visual assessment of drought stress symptoms according to a standard evaluation system (r = 0.66). Canopy-temperature-based indices were also significantly correlated with grain yields of the stress treatment (r from −0.55 to −0.63). No trait of aeroponically grown plants was correlated with traits of stressed plants in the field. We conclude that visual scoring of stressed plants was the best method of screening for drought resistance, but if controlled water deficit cannot be imposed, then drought resistance may be estimated by measuring both uprooting force and grain yield.
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  • 65
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    Euphytica 49 (1990), S. 135-139 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; Xanthomonas campestris pv. oryzae ; bacterial blight ; disease ; inheritance of esistance ; dominant ; recessive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inheritance of resistance to the Punjab isolate of Xanthomonas campestris pv. oryzae of bacterial blight disease of rice was studied in seven breeding lines resistant to the disease. The results revealed that resistance in breeding lines PAU 122-73-1-4-1, PAU 164-102-1-2-1-1-1, KJT 24, IR 5657-33-2-1-2 and IR 22082-41-2-2 was controlled by single dominant genes allelic to the dominant gene which confers resistance to the Punjab isolate in Patong 32. Resistance to the Punjab isolate in breeding lines IET 7172 and RP 2151-40-1 was found to be controlled by single recessive resistance genes allelic to one of the recessive resistance genes present in BJ 1. The two genes are independently inherited and are being used to develop bacterial blight resistant varieties.
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  • 66
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    Euphytica 45 (1990), S. 191-195 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; Oryza glaberrima ; non-glutinous pollen ; glutinous pollen ; semi-sterility ; gametocidal factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Recurrent backcrossing has been carried out with a view to transfer a gene for non-glutinous endosperm from two strains of O. glaberrima (Wx g /Wx g ) to glutinous japonica and indica varieties (wx/wx) of Oryza sativa. In the course of backcrosses Wx g /wx segregants were crossed with each of the two glutinous varieties of sativa as the respective recurrent male parent. The wx/wx and Wx g /wx segregants in the successive generations were consistently fully fertile and semi-sterile, respectively. The semi-sterility of Wx g /wx plants was attributable to abortion of most of the pollen grains carrying the gene wx. The nucleus but not cytoplasm was related to the semi-sterility. The Wx g /Wx plants having the gene for non-glutinous endosperm of a glaberrima strain and a japonica variety of sativa were also semi-sterile. Both wx- and Wx-megaspores in the plants heterozygous for the gene Wx g were deleteriously affected. The results could be explained by assuming that a factor tightly linked with the gene Wx g of glaberrima sterilizes gametes not carrying it in the heterozygotes and that the gametocidal action is exerted when combined with the sativa nucleus by the recurrent backcross method.
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  • 67
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    Euphytica 48 (1990), S. 177-188 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; cytoplasmic effects ; heterosis ; combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effect of sterilizing (‘WA’) cytoplasm on heterosis and combining ability for days to flowering, plant height and grain yield in rice was studied in 70 crosses and their reciprocals produced by 10 cytoplasmic male sterile (A), their maintainer (B) and seven restorer (R) lines following line x tester design. The materials consisting of 140 hybrids (70 A/R and their reciprocal 70 R/B) plus 17 parental lines (10 B + 7 R) were evaluated under six environments, created by growing in three fields with different fertilizer doses (0, 60, 120 kg N/ha) and 2 seasons (dry and wet) during 1986 at IRRI farm. Reciprocal cross effect (A/R vs R/B) were highly significant for all the three traits. Interaction of reciprocal cross effects with environments were also highly significant for yield and days to flowering. Cytoplasmic effect for yield, days to flowering and plant height were estimated by comparing A/R and R/B combination and testing the significance of difference with LSD value. In order to avoid confounding effect of spikelet sterility on yields, twenty crosses showing normal spikelet fertility were selected. Both positive and negative cytoplasmic effects were observed for the three traits. The effects were modified by environments, except for plant height. Heterosis for all three traits was also affected by cytoplasm, however, manifestation of cytoplasmic effects was higher for heterosis for days to flower than in heterosis for yield and plant height. Effect of cytoplasm was more pronounced on general combining ability effects of parents than specific combining ability effects of crosses. Among the parents two CMS lines A4 (IR54752A) and A8 (IR22107-113-3-3A) and two R lines: R2 (IR46) and R7 (IR9761-19-1) showed consistent positive effect of cytoplasm on general combining ability. These lines have given several good heterotic combinations. The study indicated the usefulness of evaluating diverse cytoplasmic sources in various nuclear genotypes bred for hybrid rice breeding program.
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  • 68
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    Euphytica 48 (1990), S. 215-218 
    ISSN: 1573-5060
    Keywords: Oryza sativa ; rice ; indica rice ; cytoplasmic-genetic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four indica cultivars viz. Kalinga-I, Ptb. 10, IR 27280-13-3-3-3 and Co. 41 were found to possess male sterile cytoplasm with fertility restoring genes while the cultivar Krishna was found to maintain the male sterility in all the cases. All the plants in the F1 of Kalinga-I × Krishna were observed to be completely male sterile and continued to show complete pollen sterility in subsequent backcross generations when backcrossed with recurring pollen parent, Krishna. Thus, it was posible to develop a new cytoplasmic-genetic male sterile line in indica rice (Krishna A) with Kalinga-I male sterile cytoplasm and this male sterile cytoplasm was found to be genetically different from others. Further, the newly developed male sterile line (Krishna A) was observed to be tolerant for low temperature at seedling stage.
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  • 69
    ISSN: 1573-5060
    Keywords: Sorghum bicolor ; sorghum ; Triticum aestivum ; wheat ; Oryza sativa ; rice ; Fraction-1-Protein inheritance ; Isoelectric focusing ; intergeneric hybrids ; Large and small sub-units ; rice × sorghum ; rice × wheat hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The polypeptide composition of Fraction-1-Protein (F1P) from rice × sorghum, rice × wheat hybrids and their respective parents have been analyzed by a microelectrofocusing method. The large sub-unit (LSU) is composed of three polypeptides and the small sub-unit (SSU) of two polypeptides in rice and sorghum parents and rice × sorghum hybrids. Similarly, LSU is composed of three polypeptides in the rice and wheat parents and rice × wheat hybrids. Two polypeptides occur in the SSU of rice parent and rice × wheat hybrids where as only one polypeptide in the wheat parent. These polypeptides also differ in their isoelectric points. Based on the previous reports of F1P inheritance in hybrids in other crops, F1P analysis of rice × sorghum and rice × wheat hybrids does not seem to be an important marker to identify such intergeneric hybrids. Since this is first such report of F1P inheritance in hybrids between distantly related plants, its implication in different modes of inheritance are discussed.
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  • 70
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    Plant and soil 126 (1990), S. 227-235 
    ISSN: 1573-5036
    Keywords: Oryza sativa ; rice ; root length density ; soil impedance ; tillage ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of various tillage methods on two wetland rice soils in the Philippines is reported. The soils differed principally in clay content, 38% for the clay loam (clayey, mixed isohyperthermic Entic Hapludoll) while 56% for the clay (clayey, mixed noncalcareous, isohyperthermic Andaqueptic Haplaquoll). This had a marked effect on their response to tillage and varying water regime. The clay soil, under field conditions, showed little change in pore size distribution or soil water behaviour with different tillage methods. Crop (Rice, Oryza sativa L., var. IR20) yields were unaffected by tillage. In contrast, tillage effects were very marked in the clay loam soil, which consisted of a greenhouse and a field trial. In the greenhouse, which experienced severe dry periods, wet tillage not only increased the moisture retentivity but also the soil impedance at soil matric potential (ψ)〈−0.01 MPa. Seasonal average ψ was 〈−1 MPa. Root length density decreased by 39% with dry tillage and by 56% with wet tillage compared with zero tillage. Grain yield however, did not vary with soil treatment. In the field, which experienced moderate dry spells, ψ varied between −0.13 and −0.48 MPa. Root length density was significantly reduced at soil impedance 〉0.75 MPa. Wet tillage increased soil moisture storage which minimized the soil impedance during the dry cycle more effectively than did dry tillage. The crop performed best under wet tillage and least under zero tillage. Wet tillage in this soil was more effective under moderate than under severe water stress conditions.
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