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  • Articles  (8)
  • Bacillus sphaericus  (4)
  • monoclonal antibody  (4)
  • Lepidoptera
  • temperature
  • Springer  (8)
  • 1985-1989  (8)
  • Process Engineering, Biotechnology, Nutrition Technology  (8)
  • 1
    Electronic Resource
    Electronic Resource
    Experimental formulations ofBacillus sphaericus for the control of anopheline and culicine larvae (1988)
    Springer
    Journal of industrial microbiology and biotechnology 3 (1988), S. 39-47 
    Details
    ISSN: 1476-5535
    Keywords: Bacillus sphaericus ; Bacillus thuringiensis varisraelensis ; Anopheles ; Culex ; Psorophora columbiae ; Microbial control ; Formulation ; Persistence of larvicidal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Four experimental formulations ofBacillus sphaericus Neide (2362 isolate) were evaluated for larvicidal activity against culicine and anopheline larvae in several natural and artificial habitats. A granular formulation (5% primary powder) was tested against natural populations of mosquitoes in two simulated habitats in Florida and in maturing and reflooded rice fields in Louisiana. Larvae ofCulex quinquefasciatus Say were reduced by 97 and 99% after application of the granules at the rate of 10 kg/ha to polluted tanks and 2.5 kg/ha to sod-lined potholes, respectively. Anopheline andPsorophora columbiae (Dyar and Knab) larvae were reduced by 68 and 92–100%, respectively, after application of 5 kg granules/ha to rice fields. A flowable concentrate (12.8% primary powder) applied to unpolluted and organically enriched habitats in Florida at 0.25 kg/ha reduced populations ofCulex spp. by 93–100% and 99%, respectively. Sustained-release briquets (5% primary powder) applied at the rate of one half briquet/1.8 m2 sod-lined potholes reduced larval populations ofCx. quinquefasciatus by 88–95% for up to 2 weeks in open sunlight. Sustained-release pellets (30% primary powder) applied to small woodland pools in Memphis, TN at the rate of four pellets/pool virtually eliminated larval populations ofCx. restuans Theobald for over 8 days. Variable persistance of larvicidal activity was noted for the other treatments depending on the formulation, target species and habitat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569441
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and description of antimicrobic-resistant (marker) strains of select insecticidal and noninsecticidal varieties ofBacillus sphaericus (1986)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1986), S. 245-250 
    Details
    ISSN: 1476-5535
    Keywords: Antibiotic-resistance ; Bacillus sphaericus ; Insecticidal strain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Single and multiple antibiotic-resistant strains were isolated from four insecticidal and two noninsecticidal parent strains ofBacillus sphaericus. Several of the single resistant strains isolated were also cross-resistant to several antibiotics of different modes of action. All of the parent strains were naturally susceptible to 11 and naturally resistant to two out of 22 antimicrobics examined. The majority, 17 out of 23, of antibiotic-resistant strains, that were isolated from the insecticidal parent strains, retained their insecticidal activity while the noninsecticidal strains remained noninsecticidal. Among the isolates were several strains that would be potentially useful as marker strains in the genetic manipulation of the insecticidal strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569278
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  • 3
    Electronic Resource
    Electronic Resource
    Initial studies of the use of aminopeptidases for the differentiation ofBacillus sphaericus strains (1987)
    Springer
    Journal of industrial microbiology and biotechnology 2 (1987), S. 285-292 
    Details
    ISSN: 1476-5535
    Keywords: Dendrogram ; Insecticidal strain ; Multilocus pattern ; Polyacrylamide gel electrophoresis ; Microbial insecicide ; Bacillus sphaericus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Analysis of aminopeptidase activity in commercially available enzyme panels (SAP-II and SAP-III) indicated qualitative differences among the eight strains ofBacillus sphaericus tested. These differences involved specific substrates utilized, the importance of sampling time, as well as differences between whole cells and cell-free extracts. Further detailed analysis of four of thel-amino acid-β-naphthylamide substrates using polyacrylamide gel electrophoresis (PAGE) demonstrated a series of allelic differences in the multilocus patterns observed. The resulting phenogram constructed from the enzyme and PAGE information showed a marked separation of the noninsecticidal strains from the insecticidal strains (58% similarity). In addition, there was a separation within the insecticidal strains of strain SSII-1, phage type 2 from strain 2297, phage type 4, and of both of these strains from the remaining highly insecticidal members of phage type 3 (strains 1593, 2013-4, 2362, 2500).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569431
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  • 4
    Electronic Resource
    Electronic Resource
    High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium (1989)
    Springer
    Cytotechnology 2 (1989), S. 5-8 
    Details
    ISSN: 1573-0778
    Keywords: hybridoma ; monoclonal antibody ; perfusion culture ; mammalian cell culture ; serum-free culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365409
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  • 5
    Electronic Resource
    Electronic Resource
    SSGF-I, a potent growth-promoting substance for mammalian cells from swine serum (1989)
    Springer
    Cytotechnology 2 (1989), S. 9-17 
    Details
    ISSN: 1573-0778
    Keywords: heterohybridoma ; LDL ; monoclonal antibody ; serum-free medium ; PEG ; swine serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A small amount of swine serum markedly stimulated cell growth for high productivity subclones derived from a mouse human-human heterohybridoma, N12-16.63, secreting an anti-tetanus toxoid human monoclonal antibody in a polyethylene glycol (PEG)-containing serum-free medium, PEG-86-1. A growth promoting substance, SSGF-I, was isolated from the serum by ammonium sulfate fractionation, Cibacron blue F3A-G affinity chromatography, DEAE-agarose ion exchange chromatography, and gel filtrations on Trisacryl GF 2000 and Sephacryl S-300. SSGF-I was characterized as a low density lipoprotein (LDL) of swine serum by its physico-chemical properties. It promoted cell growth synergistically with PEG and its optimum concentration was 1 to 100μg/ml. Human LDL was less active, and human or swine high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were inactive. Based on these results, we propose an improved serum-free medium, PEG-86-3, which contains all the ingredients of PEG-86-1 and 10μg/ml SSGF-I. This medium is useful for not only high productivity heterohybridomas but also for a variety of lymphoid cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365410
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  • 6
    Electronic Resource
    Electronic Resource
    Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium (1988)
    Springer
    Cytotechnology 1 (1988), S. 159-169 
    Details
    ISSN: 1573-0778
    Keywords: lipoprotein ; egg yolk ; serum-free medium ; cell growth ; monoclonal antibody ; ITES
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Egg yolk lipoprotein promoted growth of a wide variety of mammalian cell lines, including plasma-cytomas and epithelial cell lines, in serum-free medium. The lipoprotein was active for cell growth when used with insulin, transferrin, ethanolamine and selenite. The most active lipoprotein fraction (YLP-pI7.5) was purified to give a single peak by chromatofocusing and gel filtration, and was homogeneous on a 0.35% agarose gel electrophoretogram. The lipoprotein was characterised as a very low density lipoprotein with a protein content of only 1.3%. This lipoprotein had an optimal concentration of 300 μg/ml (4 μg protein/ml). It was easily separable from proteinous molecules secreted into the serum-free medium by the cells, since it floated on the surface of the medium after addition of ammonium sulfate, to precipitate protein, and centrifugation. An associated structure of lipid and protein seemed to be still necessary for the lipoprotein to exhibit a growth promoting activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00146817
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  • 7
    Electronic Resource
    Electronic Resource
    High cell density perfusion culture of hybridoma cells recycling high molecular weight components (1988)
    Springer
    Cytotechnology 1 (1988), S. 171-178 
    Details
    ISSN: 1573-0778
    Keywords: high-density ; hybridoma ; monoclonal antibody ; perfusion culture ; serum-free ; suspension culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media. The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00146818
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  • 8
    Electronic Resource
    Electronic Resource
    Batch and continuous culture production of the mosquito larval toxin ofBacillus sphaericus 2362 (1987)
    Springer
    Journal of industrial microbiology and biotechnology 2 (1987), S. 277-283 
    Details
    ISSN: 1476-5535
    Keywords: Bacillus sphaericus ; Microbial insecticide ; Mosquito ; Mosquito toxin ; Continuous culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Batch and continuous culture were used to investigate the production ofBacillus sphaericus mosquito larvicide. In batch culture, control of the pH at 7.2–7.3 rather than allowing the normal rise to about 8.6 decreased the toxicity of the cells. Oxygen was required for toxin formation but increasing the level of dissolved oxygen in the medium by use of pure oxygen in the gas stream lowered toxin production. Sporulation and toxin production occurred in continuous culture and were greater at lower dilution rates. However, toxin yield in continuous culture was too low to be a likely alternative to batch culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569430
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