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  • Articles  (457)
  • Rat  (286)
  • Triticum aestivum  (158)
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  • Springer  (457)
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  • 1985-1989  (457)
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  • Articles  (457)
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  • 1
    ISSN: 1435-604X
    Keywords: Brain tumour ; Rat ; Detection ; Fluorescence ; Laser ; Haematoporphyrin derivative
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract Laser-induced fluorescence has been used for the identification of brain tumours in rats, which have been previously given tumour-seeking haematoporphyrin derivative. A pulsed nitrogen laser (λ=337 nm) was used in conjunction with an optical multichannel analyzer. For both inoculated RG-2 and TCVC rat-brain-tumour models, the blue autofluorescence was strongly reduced in the tumour compared with normal brain tissue, and at the same time the characteristic red-drug signal increased. The contrast between tumour and normal tissue was strongly enhanced by forming the ratio between the two signals. Implications for possible improvement of tumour delineation in brain tumour surgery are discussed.
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  • 2
    ISSN: 1432-0789
    Keywords: Wheat ; Paddy straw compost ; N and P enrichment ; Rock phosphate ; Pyrite ; Triticum aestivum ; Nutrient uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A nutrient-rich compost from paddy straw was prepared using urea and Mussoorie rock phosphate for N and P enrichment respectively. Inorganic N was partly conserved in the compost by the addition of pyrite. Citric-acid-soluble P also increased with the addition of pyrite. Compost containing about 1.6% total N and 3.3% total P was found to be a good source of P for a wheat crop and also supplied a significant amount of N to the plants.
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  • 3
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    Current genetics 16 (1989), S. 211-218 
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Repetitive DNA ; Inversion ; Recombination ; Transposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction mapping and DNA sequencing were used to characterize dispersed repetitive DNA in the chloroplast genome of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. To map repeat families, chloroplast DNA (cpDNA) clones were hybridized at high stringency to one another and to cpDNA cut with restriction enzymes. Repeats are clustered in four regions of the genome and comprise at least six families. Sequence analysis of one repeat family shared among three XbaI fragments indicated the presence of a 633 by inverted repeat which contains a complete tRNA-Serine (GCU) gene and a highly conserved open reading frame (ORF 3.6). Both ends of this 633 bp dispersed repeat have a transposon-like combination of short direct and inverted repeats. One copy of the repeat flanks one of the endpoints of a major inversion which differentiates Douglas-fir from tobacco cpDNA. Dispersion of repetitive DNA by transposition, coupled with loss of the large inverted repeat, appears to have predisposed conifer cpDNA to a number of inversions. An 8 by (CATCTTTT) direct repeat in tobacco is located between two inverted sections in Douglas-fir; it may be a target sequence for homologous recombination.
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  • 4
    ISSN: 1432-1432
    Keywords: Carcinoembryonic antigen ; Evolution ; Gene family ; Human ; Rat ; Synonymous substitutions ; Silent molecular clock ; Evolutionary trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Various rodent and primate DNAs exhibit a stronger intra- than interspecies cross-hybridization with probes derived from the N-terminal domain exons of human and rat carcinoembryonic antigen (CEA)-like genes. Southern analyses also reveal that the human and rat CEA gene families are of similar complexity. We counted at least 10 different genes per human haploid genome. In the rat, approximately seven to nine different N-terminal domain exons that presumably represent different genes appear to be present. We were able to assign the corresponding genomic restriction endonuclease fragments to already isolated CEA gene family members of both human and rat. Highly similar subgroups, as found within the human CEA gene family, seem to be absent from the rat genome. Hybridization with an intron probe from the human nonspecific cross-reacting antigen (NCA) gene and analysis of DNA sequence data indicate the conservation of noncoding regions among CEA-like genes within primates, implicating that whole gene units may have been duplicated. With the help of a computer program and by calculating the rate of synonymous substitutions, evolutionary trees have been derived. From this, we propose that an independent parallel evolution, leading to different CEA gene families, must have taken place in, at least, the primate and rodent orders.
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  • 5
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    Nutrient cycling in agroecosystems 20 (1989), S. 59-66 
    ISSN: 1573-0867
    Keywords: Ammonia volatilization ; nitrogen leaching ; denitrification ; time of N application ; wheat ; Triticum aestivum ; Triticale ; irrigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Grain yield, nitrogen (N) assimilation, ammonia (NH3) volatilization, denitrification and fertilizer N distribution were examined in three commercially grown cereal crops; two were sown into conventionally tilled fields, while the third was direct drilled into an untilled field. The crops were top dressed with urea at establishment, tillering or ear initiation. Crop yield and N assimilation were measured in 16 m by 2.5 m plots receiving 0, 35, 70, 105, 140 or 175 kg N ha−1. A mass balance micrometeorological technique was used to measure NH3 volatilization, and other fertilizer N transformations and transfers were studied using15N labelled urea in microplots. On the conventionally tilled sites application of urea increased the grain yield of wheat from 3.9 to 5.5 t ha−1, when averaged over the five application rates, three application times and two sites. There were no site or application time effects. However, on the direct drilled site, time of application had a significant effect on grain yield. When urea was applied at establishment, grain yield was not significantly increased and the mean yield (2.81 t ha−1) was less than that obtained from treatments fertilized at tillering or ear initiation (4.09 and 4.0 t ha−1, respectively). Much of the variation in grain yield at the no-till site could be ascribed to differences in NH3 volatilization. At the no-till site, NH3 losses were equivalent to 24, 12 and 1% of the N applied at establishment, tillering and ear initiation, respectively. Negligible volatilization of NH3 occurred at the other sites. The surface soil at the no-till site had the highest urease activity and the soil was covered with alkaline ash resulting from stubble burning. Plant recovery of fertilizer N did not vary with application time on conventionally tilled sites (mean 62%). However, plant recovery of15N applied to the no-till site at establishment (35% of the applied N) was significantly less than that from plots where the application was delayed (45% at tillering and 55% at ear initiation, respectively). Leaching of N to below 300 mm depth was minimal (0 to 5% of the applied N). The calculated denitrification losses ranged from 1% to 14% of the applied N. The results show that the relative importance of NH3 volatilization, leaching and denitrification varied with site and fertilization time. The importance of the various N loss mechanisms needs to be taken into account when N fertilization strategies are being developed.
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  • 6
    ISSN: 1432-2145
    Keywords: Pollen development ; Triticum aestivum ; RH0007
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A study of pollen development in wheat was made using transmission electron microscopy (TEM). Microspores contain undifferentiated plastids and mitochondria that are dividing. Vacuolation occurs, probably due to the coalescence of small vacuoles budded off the endoplasmic reticulum (ER). As the pollen grain is formed and matures, the ER becomes distended with deposits of granular storage material. Mitochondria proliferate and become filled with cristae. Similarly, plastids divide and accumulate starch. The exine wall is deposited at a rapid rate throughout development, and the precursors appear to be synthesized in the tapetum. Tapetal cells become binucleate during the meiosis stage, and Ubisch bodies form on the plasma membrane surface that faces the locule. Tapetal plastids become surrounded by an electron-translucent halo. Rough ER is associated with the halo around the plastids and with the plasma membrane. We hypothesize that the sporopollenin precursors for both the Ubisch bodies and exine pollen wall are synthesized in the tapetal plastids and are transported to the tapetal cell surface via the ER. The microspore plastids appear to be involved in activities other than precursor synthesis: plastid proliferation in young microspores, and starch synthesis later in development. Plants treated with the chemical hybridizing agent RH0007 show a pattern of development similar to that shown by untreated control plants through the meiosis stage. In the young microspore stage the exine wall is deposited irregularly and is thinner than that of control plants. In many cases the microspores are seen to have wavy contours. With the onset of vacuolation, microspores become plasmolyzed and abort. The tapetal cells in RH0007-treated locules divide normally through the meiosis stage. Less sporopollenin is deposited in the Ubisch bodies, and the pattern is less regular than that of the control. In many cases, the tapetal cells expand into the locule. At the base of one of the locules treated with a dosage of RH0007 that causes 95% male sterility, several microspores survived and developed into pollen grains that were sterile. The conditions at the base of the locule may have reduced the osmotic stress on the microspores, allowing them to survive. Preliminary work showed that the extractable quantity of carotenoids in RHOOO7-treated anthers was slightly greater than in controls. We concluded that RH0007 appears to interfere with the polymerization of carotenoid precursors into the exine wall and Ubisch bodies, rather than interfering with the synthesis of the precursors.
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  • 7
    ISSN: 1432-1351
    Keywords: Rat ; Melatonin ; Circadian rhythm ; 5-hydroxytryptophan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rhythm in melatonin production in the rat is driven by a circadian rhythm in the pineal N-acetyltransferase (NAT) activity. Rats adapted to an artificial lighting regime of 12 h of light and 12 h of darkness per day were exposed to an 8-h advance of the light-dark regime accomplished by the shortening of one dark period; the effect of melatonin, triazolam and fluoxetine, together with 5-hydroxytryptophan, on the reentrainment of the NAT rhythm was studied. In control rats, the NAT rhythm was abolished during the first 3 cycles following the advance shift. It reappeared during the 4th cycle; however, the phase relationship between the evening rise in activity and the morning decline was still compressed. Melatonin accelerated the NAT rhythm reentrainment. In rats treated chronically with melatonin at the new dark onset, the rhythm had already reappeared during the 3rd cycle, in the middle of the advanced night, and during the 4th cycle, the phase relationship between the evening onset and the morning decline of the NAT activity was the same as before the advance shift. In rats treated chronically with melatonin at the old dark onset or in those treated with melatonin 8 h, 5 h and 2 h after the new dark onset during the 1st, 2nd and 3rd cycle, respectively, following the advance shift, the NAT rhythm reappeared during the 3rd cycle as well but in the last third of the advanced night only. Neither triazolam nor fluoxetine together with 5-hydroxytryptophan administered around the new dark onset facilitated NAT rhythm reentrainment after the 8-h advance of the light-dark cycle.
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  • 8
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    Theoretical and applied genetics 78 (1989), S. 728-734 
    ISSN: 1432-2242
    Keywords: Esterases ; Genetic variability ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic variability of endosperm esterase has been studied in 42 cultivars of Triticum aestivum L. 2n=6x=42. Different techniques, including sequential electrophoresis and electrofocusing, have been used with various substrates and esterase inhibitors. The electrophoretic patterns in each cultivar are described. Chromosomal location using the nullitetrasomic and ditelosomic lines of Chinese Spring was carried out in order to relate and/or locate the esterase genes to specific chromosomes. Most of the esterase isozymes located were in the long arm of the chromosomes of the homoeology group 3; but we have found six located in the short arms, five of them in the chromosome 3AS and one in the 3DS. This location increases the number of esterase genes described, because no esterase genes had been described so far in short arms of chromosomes of the homoeology group 3. The genetic control is discussed and, according to our results, between 12 and 15 loci, organized in five “compound loci”, control the endosperm esterases in wheat. Also one “modifier” gene modifying the mobility of two esterase bands and present in all the cultivars studied is postulated.
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  • 9
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    Theoretical and applied genetics 78 (1989), S. 873-878 
    ISSN: 1432-2242
    Keywords: Heat shock proteins ; 2D electrophoresis ; Triticum aestivum ; Gene location ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The low molecular weight heat shock protein (HSP) profiles of the hexaploid wheat cultivar “Chinese Spring” and its ditelosomic series were characterized by isoelectric focusing polyacrylamide gel electrophoresis of denatured in vivo radiolabeled proteins. Comparisons of the ditelosomics (DTs) to the euploid “Chinese Spring” enabled the assignment of genes controlling 9 of the 13 targeted HSPs to seven chromosome arms. There did not appear to be a genome-specific action in the regulation of expression of these HSPs. There did appear to be a higher frequency of controlling genes within homoeologous DT lines 3, 4 and 7. Significant variation in protein quantity was evident among the DT lines for some HSPs, while other HSPs were remarkably stable in their expression across all DTs examined. The results are useful in identifying specific DT lines for the investigation of HSP functions in hexaploid wheat.
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  • 10
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron ; (Elytrigia, Thinopyrum) ; Intergeneric hybridization ; Crossability ; Wide crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids between Triticum aestivum L. and conventional rhizomatous Agropyron species were produced in variable frequencies. They were recovered in high percentage frequencies for T. aestivum cultivars with A. acutum (14.6%), A. intermedium (48.0%), A. pulcherrimum (53.3%), and A. trichophorum (46.6%). The crossability percentages with the highly crossable cultivar ‘Chinese Spring’ for these Agropyron species accessions were 33.12%, 65.0%, 53.3%, and 65.4%, respectively. Autosyndetic associations of two of their three genomes gave mean meiotic chromosome association data of 17.0 I (univalents) +1.53 II (ring bivalents) + 7.04 II (rod bivalents) +1.43 III (trivalents) +0.05 IV (quadrivalents) +0.01 IV (pentavalents) for A. acutum and of 21.8 I + 1.56 II (rings) +7.22 II (rods) +0.84 III + 0.04 IV for A. intermedium. Chromosome pairing at metaphase I was comparatively lower for A. pulcherrimum (34.4 I + 0.2 II (rings) +3.4 II (rods) +0.14 III) and A. trichophorum (36.7 I + 0.35 II (rings) +2.26 II (rods) + 0.04 III) hybrids with T. aestivum. Hybrids of wheat with A. campestre and A. repens were obtained in low frequency. Direct crossing did not permit T. aestivum/ A. desertorum hybridization. However, by utilizing the 2n=10x=70 A. repens/A. desertorum amphiploid as the pollen source, hybridization with T. aestivum did indeed occur. Aneuploidy was prevalent in this hybrid combination while all other hybrid combinations were apparently normal.
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  • 11
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    Theoretical and applied genetics 78 (1989), S. 229-232 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Puccinia recondita ; Adult plant resistance ; Resistance genes ; Durable resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Wheat genotypes, including backcross derivatives of ‘Thatcher’ carrying Lr10 and Lr23, substitution lines for Lr10 and Lr23 in Chinese Spring background and Chinese Spring and Thatcher were analysed against 21 pathotypes of leaf rust in seedling tests. Adult plant responses in all these stocks were observed in the field nurseries under exposure to the inoculum of the Indian virulent races of leaf rust. The seedling data demonstrated that both the substitution lines and the backcross derivatives for each gene carry identical pattern of infection for resistance. The high level of adult plant resistance in the substitution lines, in contrast to the backcross derivatives in Thatcher, has been postulated to be due to the combination of resistance contributed by Lr10 and adult plant Chinese Spring resistance or to Lr23 and Chinese Spring adult plant resistance. It has been suggested that genes Lr10 and Lr23 added to the Chinese Spring background provide sources for durable resistance, since Chinese Spring has continued to provide a moderate level of adult plant resistance to leaf rust for a very long time.
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  • 12
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    Theoretical and applied genetics 78 (1989), S. 521-524 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; SCEs ; Auxin ; Cytokinin ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to know the mutagenic effects of synthetic auxins (NAA, 2,4-D, and 2,4,5-T) and a cytokinin (kinetin) in vitro, sister chromatid exchanges (SCEs) were analyzed in cultured cells of a hexaploid wheat (Triticum aestivum L.). In the MS medium supplemented with 2.0 mg/l 2,4-D, the mean number of SCEs per cell was 15.2, and per pg of DNA, 0.42. No significant effect was found in the treatments of NAA or 2,4-D at concentrations of 0.5–10.0 mg/l, whereas more than 2.0 mg/l of 2,4,5-T induced dramatic increases of SCEs. Kinetin itself had no significant effect on SCE induction, but there was a tendency that SCEs induced by 2,4,5-T were suppressed by kinetin.
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  • 13
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    Theoretical and applied genetics 78 (1989), S. 625-632 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Tissue culture ; Callus ; Monosomic analysis ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ability of immature embryos of wheat (Triticum aestivum L.) to respond in cell culture was examined in crosses between the ‘Wichita’ monosomic series and a highly regenerable line, ‘ND7532’. Segregation in disomic controls and 13 monosomic families showed a good fit to a monogenic ratio indicating a qualitative mode of inheritance. Segregation in the cross involving monosomic 2D showed a high frequency of regeneration (93.6%) and high callus growth rate (1.87 g/90 days) indicating that 2D is a critical chromosome. Modifying genes may be located on other chromosomes. Substitution of chromosomes from a low regenerable cultivar ‘Vona’ further indicated that the group 2 chromosomes, in particular chromosome 2D, possess genetic factors promoting callus growth and regeneration.
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  • 14
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    Cell & tissue research 256 (1989), S. 553-558 
    ISSN: 1432-0878
    Keywords: Transforming growth factor (TGF)-β ; Myocardium ; mRNA ; Fibroblast ; Cardiomyocyte ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transforming growth factor-β (TGF-β) is a biologically active polypeptide present in normal tissues as well as transformed cells. Two structurally related forms of this peptide are TGF-β 1 and TGF-β 2. Using freshly isolated cardiomyocytes and non-myocyte heart cells, and a [32P]-labelled cDNA probe to human TGF-β 1, we demonstrated that mRNA for TGF-β 1 could be detected only in the nonmyocyte fraction of heart cells. In the present study, the distribution of TGF-β 1 in the heart was determined by immunofluorescence staining by use of a polyclonal antibody to porcine TGF-β 1 in cryostat sections of rat heart. Immunofluorescence staining was intense around the blood vessels and radially diffuse in the surrounding myocardium.
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  • 15
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    Cell & tissue research 256 (1989), S. 303-307 
    ISSN: 1432-0878
    Keywords: Arachnoid cells ; Tight and gap junctions ; Cold injury ; Ultrastructure ; Freeze-fracture technique ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The junctional complexes of cells in the outer arachnoid layer overlying the cerebral cortex of 2-week-old rats were examined with freeze-fracture electron microscopy up to 60 min after transcranial cold injury to the dorsal surface of the brain. Within 30 min after injury, areas of gap and tight junctions with morphological features characteristic of junction formation and/or junction disruption were found scattered among normal junctional complexes in some arachnoid cells. Within 60 min after injury, tight junctions with features typical of less leaky zonulae occludentes were present in all arachnoid cells examined. These morphological features include increases in the number of tight junctional strands and the number of strand-to-strand anatomoses. Gap junctions were interspersed among the tight junctional strands, and many were completely encircled by the strands. The increase in the number and complexity of the tight junctional strands in response to brain injury may be the morphological basis for the maintenance of the cerebrospinal fluid-blood dural barrier.
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  • 16
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    Cell & tissue research 256 (1989), S. 559-565 
    ISSN: 1432-0878
    Keywords: Retrograde tracing ; Immunocytochemistry ; Vascular innervation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The origin of nerve fibers to the superficial temporal artery of the rat was studied by retrograde tracing with the fluorescent dye True Blue (TB). Application of TB to the rat superficial temporal artery labeled perikarya in the superior cervical ganglion, the otic ganglion, the sphenopalatine ganglion, the jugular-nodose ganglionic complex, and the trigeminal ganglion. The labeled perikarya were located in ipsilateral ganglia; a few neuronal somata were, in addition, seen in contralateral ganglia. Judging from the number of labeled nerve cell bodies the majority of fibers contributing to the perivascular innervation originate from the superior cervical, sphenopalatine and trigeminal ganglia. A moderate labeling was seen in the otic ganglion, whereas only few perikarya were labeled in the jugular-nodose ganglionic complex. Furthermore, TB-labeled perikarya were examined for the presence of neuropeptides. In the superior cervical ganglion, all TB-labeled nerve cell bodies contained neuropeptide Y. In the sphenopalatine and otic ganglia, the majority of the labeled perikarya were endowed with vasoactive intestinal polypeptide. In the trigeminal ganglion, the majority of the TB-labeled nerve cell bodies displayed calcitonin gene-related peptide, while a small population of the TB-labeled neuronal elements contained, in addition, substance P. In conclusion, these findings indicate that the majority of peptide-containing nerve fibers to the superficial temporal artery originate in ipsilateral cranial ganglia; a few fibers, however, may originate in contralateral ganglia.
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  • 17
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    Cell & tissue research 256 (1989), S. 447-456 
    ISSN: 1432-0878
    Keywords: MAP2 ; Actin ; Dendritic spines ; Spine apparatus ; Spine synapses ; Postsynaptic density ; Synaptic plasticity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment showing MAP2 immunoreactivity. MAP2 immunolabeling was mainly associated with filaments which reacted with a monoclonal anti-actin antibody. Also, by immunogold double-labeling we colocalized MAP2 with actin on the endomembranes of the spine apparatus, smooth endoplasmic reticulum, and in the postsynaptic density. Labeling was nearly absent in axons and axonal terminals. These results indicate that MAP2 is an actin-associated protein in dendritic spines. Thus, MAP2 may organize actin filaments in the spine and endow the actin network of the spine with dynamic properties that are necessary for synaptic plasticity.
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  • 18
    ISSN: 1432-0878
    Keywords: Adrenal medullary endothelial cells ; Pheochromocytoma (PC12) cells ; Co-culture ; Cell surface extracts ; Adhesion ; Cell-cell interactions ; Bovine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chromaffin cells in the adrenal medulla are found in close proximity to capillary endothelial cells, thereby forming the classical endocrine complex. To examine the possible chemical basis of their interaction in more detail, we have grown bovine adrenal medullary endothelial (BAME) cells in monolayer cultures and added to them pheochromocytoma (PC12) cells, a chromaffin tumor cell line of rats. The PC12 cells were chosen because of the similarities they share with adrenal medullary chromaffin cells. PC12 cells rapidly attached to BAME cells cultures, their rate of adhesion being significantly enhanced over binding of PC12 cells to either uncoated plates or to monolayers of unrelated cell cultures. Consistent with this observation, we noted that the extracellular matrix (ECM) derived from the BAME cells did not enhance PC12 cell adhesion and did not promote neurite sprouting as previously described for ECM derived from corneal endothelial cells. The specific adhesion between PC12 and BAME cells could be abolished by cell surface extracts derived from these two cells but not by extracts derived from unrelated cell types. This activity was heat-labile, sensitive to trypsin and, to a lesser extent, to neuraminidase. We therefore conclude that PC12 cells may interact with BAME cells by specific proteinaceous adhesive factors associated with their plasma membranes. These interactions might represent the formative role of cell-cell contacts in the organization of the developing adrenal gland.
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  • 19
    ISSN: 1432-0878
    Keywords: SEM ; TEM ; Interstitial cell ; Myenteric plexus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An extensive cellular network becomes visible over the myenteric plexus of the rat after removal of the overlying tissues under the scanning electron microscope. The cells are mainly stellate and have many slender processes via which they interconnect. They form a three-dimensional network and are closely associated with the ganglia and nerve bundles, and also extend over the smooth muscle cells. They are considered to correspond to the interstitial cells of Cajal because of their peculiar arrangement and their topography. Transmission electron-microscopic evidence demonstrates that the majority of those cells have features of fibroblasts. Gap junctions and intermediate junctions are observed between these fibroblast-like cells, and also between them and smooth muscle cells. Examination of serial thin sections reveals that single fibroblast-like interstitial cells connect to both circular and longitudinal muscle cells via gap junctions. It is suggested that the network of interstitial cells conducts electrical signals.
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  • 20
    ISSN: 1432-0878
    Keywords: Gastrin ; Gastrin-releasing peptide ; Bombesin ; Stomach ; Autonomic innervation ; Immunohistochemistry ; Guinea pig ; Rat ; Dog ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The relationship between bombesin-like immunoreactive (bombesin-LI) nerve fibres and gastrin-LI G-cells was examined in gastric antral mucosa from guineapig, rat, dog and man using a double-labelling fluorescence immunohistochemical technique. The greatest density of bombesin-LI nerve fibres was found within the basal mucosa in all species and the density of innervation decreased towards the luminal surface. Most G-cells were in a band occupying approximately the middle third of the mucosa. The proportion of G-cells found within a distance of 2 μm from bombesin-LI nerve fibres was low in all species (6% in the guinea-pig, 22% in the rat, 14% in the dog, and 9% in the human). It is proposed that the neuropeptide released from bombesin-LI antral mucosal nerve fibres traverses distances of greater than several μm to reach the target G-cells. This may be achieved by passage through the mucosal microcirculation.
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  • 21
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    Cell & tissue research 257 (1989), S. 263-268 
    ISSN: 1432-0878
    Keywords: Retinol ; Vacuoles ; Immunohistochemistry ; Plasma proteins ; Hepatocytes ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The vacuoles occurring in rat hepatocytes after intraportal injection of retinol (33 or 67 μg) were examined immunohistochemically using respective antibodies against rat albumin, human retinol-binding protein, human ceruloplasmin, human α 1-antitrypsin, human transferrin, and human prealbumin as representative plasma proteins. The occurrence of the vacuoles reached a numerical maximum 30 min after injection of 67 μg retinol, followed by a temporal decrease. Hepatocytes from control rats, which had been intraportally injected with either blood plasma diluted to 2/3 concentration or with retinol palmitate solvent (castor oil) dissolved in blood plasma, showed immunoreactive fine granules without the occurrence of vacuoles in the cytoplasm. Identical vacuoles in serial sections appeared immunohistochemically either immunoreactive or non-immunoreactive for all the antibodies used, with rare exceptions. The occurrence of several rare exceptions suggested that 2 kinds of vacuoles might be formed in different cytoplasmic compartments. A zonal distribution of vacuoles was apparent in the hepatic laminae (or acini) within the liver lobules. The vacuoles were predominantly distributed in zone 2, and to a lesser extent in zone 3 and zone 1 in that order.
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  • 22
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    Protoplasma 152 (1989), S. 14-21 
    ISSN: 1615-6102
    Keywords: Differentiation ; Heterochronic lysis ; Polarity ; Root protophloem sieve elements ; Triticum aestivum ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Developing protophloem sieve elements in roots of wheat are arranged in single vertical files. In the last immature differentiating sieve element bearing ribosomes the proximal end of the cytoplasm displays a diluted appearance in contrast to the distal end where the cytoplasm exhibits a considerably increased electron density. Differences can also be observed in ribosome quantity, organelle ultrastructure and the time of initiation of cell component degradation, those at the proximal end disorganizing first, suggesting a nonsimultaneous disorganization of the cell components in the two areas. This phenomenon, termedheterochronic lysis, is presumably an expression of an existing polarity not detectable in younger stages, but it might also be the result of an asynchronous enzymatic activity.
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  • 23
    ISSN: 1573-5036
    Keywords: copper sources ; grain yield ; methods of application ; park wheat ; stem melanosis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Park wheat (Triticum aestivum cv. Park) is susceptible to stem melanosis when grown on Cu-deficient soils. Three field experiments were conducted to evaluate the effectiveness of various Cu sources, using different methods and rates of application for reducing the incidence of stem melanosis and increasing the grain yield of Park wheat on a Cu-deficient Black Chernozemic soil in central Alberta. A fourth experiment determined the residual effect of Cu over 4 years. In the first experiment, Park wheat had less disease and higher grain yield when Cu-chelate solution at 2 and 4 kg Cu ha−1 or Cu-sulfate solution at 10 and 20 kg Cu ha−1 was applied to the soil surface and incorporated to a depth of 8 cm or was applied at these rates as a foliar spray. The foliar application, however, was phytotoxic and delayed maturity by approximately two weeks. Sidebanding Cu (4 cm to the side and 4 cm below the seed row) was least effective. In the second experiment, Cu-sulfate solution incorporated into the soil was more effective than the soil incorporated granular Cu-sulfate in reducing disease incidence and increasing grain yield in the year of application. In the following year the granular Cu was as effective as solution Cu. The third experiment showed that Cu seed dressings did not have any effect on disease incidence or grain yield of Park wheat. In the fourth experiment, the residual effect of Cu-chelate was evident four years after application. The grain yield in the fourth year was about four times that of the control.
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  • 24
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; near-isogenic lines ; phenotypic similarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The degree of phenotypic similarity of Centana and its near-isogenic lines (NILs) has been investigated. Some NILs have a disease reaction, a gliadin pattern and a yield level which differs from Centana. This means that they still possess many donor genes. One of the NILs, Shortana, must be a derivative of a backcross line crossed with an unknown genotype resulting in a good yielding variety.
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  • 25
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    Euphytica 40 (1989), S. 259-264 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum durum ; durum wheat ; pentaploid hybrids ; homoeologous pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The meiotic behavior of 22Triticum turgidum durum × Triticum aestivum L. cv. Chinese Spring (2n=35 chromosomes, AABBD genomes) F1 hybrids has been analyzed and compared with that of the same cultivars hybridized withT. turgidum durum cv. Senatore Capelli. The multivalent associations observed in the hybrids may be attributed to a large extent to homoeologous pairing. Our study shows that not all the chromosomes of the D genome have the same affinity with their homoeologues of the A or B genomes. We have also concluded that there is no correlation between the percentage of the cells with multivalent associations and the mean of chiasmata per cell (attached arm).
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  • 26
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    Euphytica 41 (1989), S. 235-246 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; landrace groups ; classification ; primary landrace ; secondary landrace ; Austria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using 49 morphological characteristics sixty-six accessions originating from 15 populations of bread wheat, together with 8 selections from landraces and 5 improved varieties were classified in five groups: group 1: Alpiner Bartwizen (including Hausbergweizen), Attergauer Bartweizen, which are marked by awned white ears; group 2: Awned Sipbachzeller (and its selection Otterbacher Bartweizen) and marked by awned red ears. This group also includes some awned derivatives of hybrids between group 1 and group 3; group 3: Sipbachzeller wheat, including Innviertler wheat and the selections Ritzlhofer Alt, Achleitner and Wieselburger roter Kolben; group 4: Tassilo and Tassilo-variants, and group 5: Dickkopf (Squarehead) types with lax ear, probably being derivatives of hybrids between group 3 and Dickkopf types. Most populations were contaminated with foreign material. The contamination may have been caused by the farmer who actually mixed varieties, or may have originated on the farmer's field.
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  • 27
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    Euphytica 42 (1989), S. 41-44 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; frost resistance ; locus Fr1 ; recombinant lines ; chromosome 5A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A gene for frost resistance on chromosome 5A of wheat was located using single chromosome recombinant lines from the cross between the substitution line Hobbit (Triticum spelta 5A) and Hobbit. In this sample of recombinant lines the locus for frost resistance, designated Fr1, is completely linked to the locus Vrn1 controlling vernalisation requirement. The results can be explained by a pleiotropic action of the Vrn1 locus or close genetic linkage between Vrn1 and Fr1. Further detailed study is necessary to resolve these alternative hypotheses.
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  • 28
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dwarfing genes ; drought stress ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Near-isogenic tall (no dwarfing gene), semidwarf (Rht1 or Rht2) and dwarf (Rht1 + Rht2 or Rht3) spring wheat lines were evaluated for yield and yield components under irrigated and rainfed conditions. Under irrigated conditions, the dwarf and the semidwarf lines exhibited a significant yield advantage over the tall lines. Under rainfed conditions, the semidwarf lines outyielded the tall as well as the dwarf lines. Percent yield reduction in response to drought stress was highest with the dwarfs and lowest with the tall lines. Dry matter production of the tall lines and that of the semidwarf lines did not differ significantly and both produced significantly more dry matter than the dwarf lines under irrigated as well as rainfed conditions. Plant height and kernel weight decreased with increasing degree of dwarfness while number of kernels per spikelet, harvest index and days to heading increased under both moisture regimes. The dwarfing genes did not have any significant influence on number of tillers/m2 and spikelets per spike in either moisture regime.
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  • 29
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; chromosome substitution ; frost resistance ; hardening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The highly frost resistant wheat variety Cheyenne (donor) and the poorly frost resistant variety Chinese Spring (recipient) were frozen at −9° C and −11° C at various stages of hardening, as were a number of substitution lines of these two varieties (CS/Ch 3A, CS/Ch 5A, CS/Ch 7A, CS/Ch 2B, CS/Ch 4B, CS/Ch 5B, CS/Ch 4D, CS/Ch 5D). Chromosomes 5A, 5B, 5D, 4B and 7A of Cheyenne increased the frost resistance of the recipient variety to varying extents. However, the frost resistance changed not only as a function of the different chromosomes, but also as a function of the duration of hardening, indicating that genes responsible for frost resistance are expressed differently during different phases of the hardening process.
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  • 30
    ISSN: 1573-5060
    Keywords: Triticum durum ; durum wheat ; Triticum aestivum ; wheat germplasm ; collection ; yield components ; drought response ; canopy temperature ; osmotic adjustment ; phenology ; Israel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Diverse landraces of wheat, collected from the semi-arid (150 to 250 mm of total annual rainfall) Northern Negev desert in Israel were considered as a potential genetic resource of drought resistance for wheat breeding. These materials were therefore evaluated for their reponses to drought stress in agronomical and physiological terms. Up to 68 landraces, comprising of Triticum durum, T. aestivum, and T. compactum were tested in two field drought environments, in one favourable field environment, under post-anthesis chemical plant desiccation which revealed the capacity for grain filling from mobilized stem reserves, under a controlled drought stress in a rainout shelter and in the growth chamber under polyethylene glycol (PEG)-induced water stress. Biomass, grain yield and its components, harvest index, plant phenology, canopy temperatures, kernel weight loss by chemical plant desiccation, growth reduction by PEG-induced drought stress and osmotic adjustment were evaluated in the various experiments. Landraces varied significantly for all parameters of drought response as measured in the different experiments, which was in accordance to their documented large morphological diversity. Variation in grain yield among landraces under an increasing drought stress after tillering was largely affected by spike number per unit area. Kernel weight contributed very little to yield variation among landraces under stress, probably because these tall (average of 131 cm) landraces generally excelled in their capacity to support kernel growth by stem reserve mobilization under stress. Yield under stress was reduced with a longer growth duration of landraces only under early planting but not under late planting. Landraces were generally late flowering but they were still considered well adapted phenologically to their native region where they were always planted late. Landraces differed significantly in canopy temperature under drought stress. Canopy temperature under stress in the rainout shelter was negatively correlated across landraces with grain yield (r=0.67**) and biomass (r=0.64**) under stress. Canopy temperature under stress in the rainout shelter was also positively correlated across landraces (r=0.50**) with canopy temperature in one stress field environment. Osmotic adjustment in PEG-stressed plants was negatively correlated (r=−0.60**) with percent growth reduction by PEG-induced water stress. It was not correlated with yield under stress in any of the experiments. In terms of yield under stress, canopy temperatures and stem reserve utilization for grain filling, the most drought resistant landrace was the ‘Juljuli’ population of T.durum.
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  • 31
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust resistance ; alien gene transfers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nine transfers of leaf rust (Puccinia recondita Rob. ex Desm.) resistance to wheat (Triticum aestivum L.) from Agropyron elongatum Host. Beauv., Triticum speltoides Tausch and rye (Secale cereale L.) were backcrossed up to 10 times to commercial wheat cultivars. The objective was to study the effect of the transfers on agronomic and quality characters and to make them available in desirable genetic backgrounds. The results varied greatly for different transfers. In four cases no promising material was obtained even after nine backcrosses. However, for the remaining five transfers material with potential as a new cultivar was obtained.
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  • 32
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; gene action ; oligogenic inheritance ; transgressive segregation ; number of genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between the highly susceptible Little Club and the partially resistant cultivars Westphal 12A, Akabozu and BH 1146 to obtain F1, F2 and backcross generations. Latency period (LP) was determined in plants inoculated at the young flag leaf stage with a monospore culture of race ‘Flamingo’ of wheat leaf rust. Broad sense heritability of LP in the F2 averaged 0.8. The genes showed partial to almost complete recessive inheritance. Scaling tests indicated that additive gene action was the most important factor in the inheritance of partial resistance. The tests showed that there were no indications for additive x additive, additive x dominance or dominance x dominance interactions. The number of effective factors was estimated as one or two for Akabozu, three or more for Westphal 12A, and two or three for BH 1146. BH 1146 also possessed a (semi-)dominant gene for a lower infection type which was temperature sensitive in its expression. The genes of the various parents had unequal effect on LP.
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  • 33
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; oligogenic inheritance ; transgressive segregation ; number of genes ; heritability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three partially resistant spring wheat cultivars, with a long latency period were crossed among each other and with the highly susceptible Little Club, with a very short latency period. Parents, F3 and F5 plants have been inoculated with the leaf rust race Flamingo in the young flag leaf stage to determine the latency period. From the crosses with Little Club, it was concluded that Westphal 12A carries three, Akabozu two and BH 1146 two or three genes for a longer latency period. BH 1146 appears to carry also one hypersensitive resistance gene. Transgressive segregation occurred in crosses between partially resistant cultivars. From crosses between the partially resistant cultivars, it was concluded that the genes in Akabozu and Westphal 12A are different, while those in Akabozu and BH 1146 are at least partly different. The possibilities of accumulation of LP-prolonging genes are discussed.
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  • 34
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; anther culture ; callus initiation ; cytoplasm ; cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nuclear and cytoplasmic factors affect tissue culture response in wheat (Triticum aestivum), and cytoplasmic male sterility may enhance callus initiation in anther culture. Three wheat nuclear genotypes, each in normal and two alien cytoplasms conferring cytoplasmic male sterility, were evaluated for callus initiation frequency in anther culture. Nuclear genotype had the greatest effect on callus initiation, but cytoplasm and nucleus X cytoplasm interaction also produced significant effects. The nuclear genotype of ‘Chris’ outperformed ‘Butte’ and ‘Coteau’ in all cytoplasms. Ordinary wheat and Triticum timopheevi cytoplasms outperformed Aegilops speltoides cytoplasm. Cytoplasmic male sterility did not increase callus initiation. This suggests manipulating the nuclear genotype is the best strategy for improving the capacity of wheat to initiate callus in anther culture.
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  • 35
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    Euphytica 40 (1989), S. 103-109 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; triticale ; wheat-triticale hybrids ; Secale cereale ; rye ; Gaeumannomyces graminis ; take-all ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifteen triticale and wheat-triticale hybrid lines were evaluated for resistance to the take-all fungus Gaeumannomyces graminis var. tritici and compared with five wheat and two rye lines in inoculated field and pot trials. The triticale and wheat-triticale hybrid lines varied in rye chromosome number and degree of resistance expressed. One line, Venus with seven pairs of rye chromosomes consistently showed levels of resistance intermediate between wheat and rye. A trend was observed where increasing rye chromosome content led to greater resistance but exceptions showed that variation within triticales could not be ascribed to rye chromosome content alone.
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  • 36
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; gliadins ; electrophoresis ; cultivar identification ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The process of the separation of gliadin proteins of wheat, using polyacrylamide gel electrophoresis, was studied in detail. It was shown that electrophoresis is a dynamic process during which the pH of the gel changes together with the potassium ion concentration. The most positive effect on the separation of the gliadins was generated by a moving front, which is a boundary between regions with a low concentration of K+ ions and a low pH, and region with a high concentration of K+ ions and a high pH, after optimization of the concentrations of the cations and anions in the electrode solutions. The finding was exploited for the development of an extremely simple electrophoresis system, in which buffers were not needed for obtaining a high resolution. The system was further improved by applying a stacking gel. The advantages of this system are discussed. This new approach may be useful for improving electrophoresis systems for other applications.
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  • 37
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    Euphytica 43 (1989), S. 53-57 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; gibberellic acid insensitivity ; identification of dwarfing genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary GA-insensitive Rht genes of 18 Japanese modern varieties and landraces were identified. Out of 12 modern varieties tested 6 carried only Rht1, and the other 6 carried only Rht2. No varieties carried both Rht1 and Rht2 or Rht3. The geographical distribution of the Rht genotypes in the Japanese modern varieties was clearly localized. All 6 landraces tested carried only Rht2.
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  • 38
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Scalavatis bread wheat ; durum-like hexaploid wheat ; contamination ; variation ; groups ; cluster analysis ; relationship ; Cyprus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The mutual resemblance of 81 hexaploid wheat plants contaminating durum fields on Cyprus was investigated by determining their phenotypes of gliadins, glutenins and peroxidase. Three groups were generated by clustering programs. Group 1 consists of plants with ears resembling those of durum plants, plants of groups 2 and 3 have a bread wheat appearance. Suggestions on the origin of these three groups are made. Group 1 may derive from (back)crosses between hexaploid and durum plants since time immemorial. If so, the hexaploid plants would have served as ‘donor’ of the D genomes, and durum plants as the ‘recurrent parent’. Groups 2 and 3 may derive from two introductions, either from two countries, or from the same country, but then in different periods.
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  • 39
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    Euphytica 44 (1989), S. 143-150 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Triticum durum ; durum ; environmental correlations ; grain yield ; plant height ; tiller number ; grain size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Environmental correlation coefficients were computed among all pairs of five traits, namely grain yield, heading date, number of tillers per m2, plant height and 1000-grain weight (grain size) using 30 Triticum durum and 30 Triticum aestivum varieties grown in 18 environments. Grain yield was significantly correlated with the other four traits in almost all of the varieties. The mean correlation coefficient over all varieties ranged from 0.58 to −0.83 for durum wheat and 0.66 to 0.88 for aestivum wheat. The correlation coefficients between heading date and the other traits were also significant, ranging from −0.45 to −0.79 in durum wheat and −0.61 to −0.85 in aestivum wheat. The correlation coefficient between number of tillers with plant height and 1000-grain weight were the smallest, 0.19−0.32 in durum wheat and 0.39−0.60 in aestivum wheat. It was concluded that agronomic practices favouring early and good stand establishment in the dry regions will favour the yield components and important adaptive traits, which contribute towards larger yields. Significant differences were found among genotypes in the environmental correlation coefficients and the associated changes in one trait as a result of changes in other traits.
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  • 40
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; infection frequency ; urediosorus size ; growth stage ; component analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Latency period (LP), infection frequency (IF) and urediosorus size (US) of leaf rust were determined on primary leaves and young flag leaves of 18 spring wheat cultivars. A large growth stage effect and a large cultivar effect on all three components were observed. Partial resistance as measured by the three components was generally better expressed in the adult plant stage than in the seedling stage. Associated variation of the components was observed: long LP, low IF and small US tended to go together. The association was not complete, cultivars with clear deviations of this association for one of the components were found suggesting the existence of at least partly different genetic factors controlling the respective components. LP measured on flag leaves gave the most reliable results and, therefore, could best be used as a selection criterion in breeding programs for partial resistance.
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  • 41
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; temperature sensitivity ; latency period ; infection frequency ; urediosorus size ; growth stage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three components of partial resistance (PR) were studied at three post-infection temperatures using seven spring wheat genotypes differing in level of PR and two different wheat leaf rust races. The components were latency period (LP), infection frequency (IF) and urediosorus size (US). The expression of LP was more sensitive to temperature than the expression of the other two components. LP-prolonging genes were better expressed at low temperatures than at high temperatures and cultivar differences tended to increase with decreasing temperature in both seedling and adult plant stages. The reaction of IF to temperature differed from that of LP and US, probably because IF is regulated by another mechanism than LP and US. It is recommended to perform PR-screening tests at low rather than at high temperatures. If temperatures are maintained at about 8–13°C (night-day), seedlings can be used to screen for PR instead of the more expensive adult plant tests. The effectiveness of PR in seedling stage at low temperatures suggests that the seedling stage may have epidemiological significance as the low temperatures (8–13°C) are relevant for seedlings in the field.
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  • 42
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    Euphytica 44 (1989), S. 225-234 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; winter wheat ; breeding ; phenology ; vernalization ; Vrn genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An international survey of wheat breeders and scientists was conducted to determine whether there was a common understanding of the term ‘winter wheat’. Response to photoperiod was mentioned by only seven of the 30 survey respondents. It was consequently suggested that it be excluded from any definition. Response to vernalization was mentioned by 28 of the 30 respondents. However, there was considerable variation in the method of quantifying this response and the degree of response necessary for wheat to be called ‘winter’. It was concluded that the only objective and internationally consistent definition of ‘winter wheat’ was a ‘genetic’ definition based on the complement of Vrn genes a wheat possesses. Varieties not possessing any of the dominant Vrn alleles appear to take a distinctively longer and more variable time to head when grown under non-vernalizing, long day conditions. These are ‘genetically winter’ wheats.
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  • 43
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    Euphytica 44 (1989), S. 273-282 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; race-specificity ; Puccinia recondita f.sp. tritici ; wheat leaf rust ; partial resistance ; hypersensitive resistance ; latency period ; disease severity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Partial resistance (PR) in wheat to wheat leaf rust (Puccinia recondita f.sp. tritici) is characterized by a slow epidemic build-up despite a susceptible infection type. Two greenhouse tests and two field tests, in which 11 spring wheat cultivars were exposed to five wheat leaf rust races, revealed some indication for race-specificity of PR. In the greenhouse, the expression of PR was highly dependent on the environment. Significant cultivar-race interactions in the first experiment were lost in the second experiment probably due to cultivar-environment and cultivar-race-environment interactions. In the polycyclic field tests several factors played a role in explaining the inconsistency of the cultivar-race interactions, such as differences in initial inoculum, genotypic differences in earliness, interplot interference or environmental conditions. One cultivar-race combination showed a significant but small interaction towards susceptibility in both field experiments. The interaction was probably too small to detect in the monocyclic greenhouse tests. The results do not conflict with the idea that a gene-for-gene relationship could exist between PR-genes in the host and genes in the pathogen. Some problems with regard to the selection of PR in wheat to wheat leaf rust are discussed.
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  • 44
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    Euphytica 44 (1989), S. 241-245 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; partial resistance ; environmental stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five spring wheat cultivars differing in partial resistance (PR) to wheat leaf rust were tested at Wageningen (the Netherlands) on a sandy and a clay site, El Batan (CIMMYT, Mexico) and Ponta Grossa (Brazil) over two years. The cultivars were Skalavatis 56, Little Club (both very susceptible), Westphal 12A, Akabozu and BH 1146 (all three with high levels of PR). The results showed that PR was expressed at all four locations in both years. The level of expression was influenced by the environment but the cultivar ranking was hardly affected. Selection for PR in the field can therefore be carried out over a wide range of environments.
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  • 45
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    Euphytica 44 (1989), S. 247-258 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; epidemiological parameters ; microfield ; race nursery ; partial resistance ; components of resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eighteen spring wheat cultivars were tested in microfields and race nurseries for their partial resistance PR to wheat leaf rust under low and high disease pressure respectively. Large differences existed between the 18 cultivars, Skalavatis 56 being the most susceptible and Ponta Grossa 1 being the most resistant cultivar. Of the three epidemic parameters, disease severity (DS) at the time that the susceptible check was severely diseased and area under the transformed disease severity curve (AUTC) and the logistic growth rate (r), AUTC and DS were highly correlated. Both seemed to be reliable estimators of PR but DS should be preferred for economical reasons. The logistic growth rate seemed to be unsuitable as an estimator of partial resistance. High and low disease pressure gave similar cultivar ranking. PR can be screened and selected equally well in race nurseries with low space, low time and low cost input as in microfields with high space, time and cost input. Cultivar differences in development rate had a large impact on the cultivar differences for amount of disease and can therefore greatly bias the estimation of cultivar resistance. The resistance of early cultivars tended to be underestimated whereas the resistance of late cultivars tended to be overestimated. The effect of differences in developmental rate was most pronounced in the flag leaf. It is advisable to avoid the assessment of disease levels on the flag leaf only and to incorporate in the tests several susceptible and resistant checks that cover the range of development rates in the material to be selected, because otherwise selection for resistance will tend to select also for lateness. Regression of the epidemiological parameters on three components of partial resistance revealed that latency period (LP) is an important factor in determining the resistance observed in the field explaining on average 67% of the observed variation. Adding infection frequency (IF) and urediosorus size (US) to the linear model increased the proportion of the observed variation in the field explained by the components to 80%. This result supports the idea that the components of PR inherit independently, at least, in part.
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  • 46
    ISSN: 1573-5036
    Keywords: allelopathy ; lyophilization ; Oryza sativa ; phytoxicity ; rhizosphere soil ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using a wheat seed bio assay, the phytotoxicity of extracts obtained from wheat and rice rhizosphere soils was determined. The wheat rhizosphere soil extract was found to show phytotoxic effects. More allelopathic materials were found from the wheat-rice rotation soils than from the rice soil. Extracts obtained under basic conditions (pH 8) were found to be more inhibitory than those obtained by acid extraction (pH 5). Wheat straw was found also to contain phytotoxic substances. A comparison of two methods in concentrating the aqueous extracts, rotary evaporation and lyophilization, indicates that the former contained more substances with phytotoxic effects.
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  • 47
    ISSN: 1573-5044
    Keywords: anther culture ; Triticum aestivum ; donor plant environment ; incubation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four different growth environments (field, two phytotron greenhouses and one growth chamber) were compared, using two genotypes of spring wheat, one recalcitrant and one responsive. Field-grown plants gave inferior results. Large improvements could be made by improving the conditions, embryoid frequencies in the two genotypes reaching 77.1% and 183.9% per 100 anthers, respectively. High light intensity during the induction phase strongly suppressed induction in both genotypes, but stimulated regeneration of green plants in the recalcitrant genotype, which had the lowest regeneration ability. Weak, diffuse light did not inhibit induction while the positive effect on regeneration was maintained. Also, another recalcitrant genotype was grown in the field, together with two F1-hybrids (recalcitrant x recalcitrant and recalcitrant x responsive). Evidence for a three-factor system was obtained.
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  • 48
    ISSN: 1573-5044
    Keywords: wheat ; Triticum aestivum ; ABA ; ABA analogs ; somatic embryogenesis ; precocious germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Zygotic embryos from ten spring wheat (Triticum aestivum L.) genotypes were tested for embryogenic callus induction in the presence or absence of externally supplied (±)-abscisic acid (ABA) and two of its analogs, methyl abscisate and methyl epoxy-beta-ionylideneacetate. (±)-ABA and its analogs suppressed precocious germination of cultured late-stage embryos and promoted embryogenic callus induction. A significantly greater number of plants was regenerated from calli induced in the presence of ABA and ABA analogs. Early-stage embryos when cultured in the presence of (±)-ABA showed a negative response. Possible roles of ABA with respect to the expression of somatic embryogenesis are discussed.
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  • 49
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    Plant cell, tissue and organ culture 18 (1989), S. 227-230 
    ISSN: 1573-5044
    Keywords: sterility ; diploid ; haploid ; pollen plants ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fertility of plants from wheat anther culture was studied. It was found that one half of the 36 plants with diploid root tips didn't set seeds at all, and that 41 of the 42 plants with haploid root tips were completely sterile. It was surprising that sterility was so widely distributed even among the plants with diploid root tips.
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  • 50
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    Journal of comparative physiology 159 (1989), S. 237-242 
    ISSN: 1432-136X
    Keywords: Amylase ; Mosquitofish ; Rat ; Drosophila ; Structure ; Function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Amylases from the mosquitofish (Gambusia affinis holbrooki, Pisces: Poeciliidae) and rat were purified and compared withDrosophila amylases in terms of structure and function. At the structural level, amino acid compositions of the three amylases were compared. At the functional level, amylase activities were compared on various substrates and in the presence of inhibitors. While the amylases from all three organisms had properties typical of alpha-amylases, both structural and functional differences were observed. Using resemblance coefficients of distance and similarity from numerical taxonomy, it was determined that the amylases from the rat andDrosophila were more similar to each other than either was to amylase from the mosquitofish, and that structural differences between the amylases did not reflect functional differences, i.e. there was no correlation between amylase structural and functional distances.
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  • 51
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    European journal of plant pathology 95 (1989), S. 85-105 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; Erysiphe graminis ; vertical distribution ; triadimefon ; AUDPC-value ; economic thresshold ; seed number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Opbrengstderving van wintertarwe door meeldauw werd bestudeerd in 11 veldproeven in Nederland. De schade bedroeg gemiddeld 0.013 (SE=0.003) kg are−1 per puistdag meeldauw per blad, vanaf het tweede-knoop stadium tot begin deegrijp bij opbrengstniveaus van 70 tot 90 kg are−1, in de blanco. Bij een ziektestress van 0 tot 2000 puistdagen meeldauw per blad werd geen afwijking van een rechtlijnig verband gevonden. De schaderelatie werd niet significant beïnvloed door de verschillende jaren, rassen of grondsoorten. Het effect van meeldauw op enkele opbrengstcomponenten werd aangetoond. Meeldauwprofielen in de onbehandelde veldjes konden worden beschreven met de vergelijking: CM=CLAb, waarin CM het cumulatieve aantal puistjes is en CLA het cumulatieve bladoppervlak, beide berekend van bovenin het bladerdek naar beneden, de totalen gestandaardiseerd op één. De gradiënt parameter b bedroeg gemiddeld 3.4 (SE=0.9). Waargenomen verschillen in steilte van de meeldauwprofielen beïnvloedde de schaderelatie niet aantoonbaar.
    Notes: Abstract Damage by mildew to winter wheat was studied in 11 field experiments in the Netherlands. Damage is described by the simple function: −0.013 (SE=0.003) kg are−1 per pustule-day of mildew per leaf, from second node stage to early dough at yield levels of 70 to 90 kg are−1, in diseasefree plots. No deviations from linearity at disease stresses from zero to two thousand pustuledays per leaf were observed. Years, cultivars or soil types did not affect the damage function significantly. The effects of mildew on some yield components were suggested. Mildew profiles in untreated plots could be described by the equation: CM=CLAb, in which CM and CLA are the cumulative pustule number and the cumulative leaf area, respectively, calculated both from top to bottom of the canopy, and totals standardized at unity. Estimates of the gradient parameter b aveaged 3.4 (SE=0.9). Observed differences in steepness of the profiles did not affect the damage function significantly.
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  • 52
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    Pure and applied geophysics 128 (1988), S. 365-399 
    ISSN: 1420-9136
    Keywords: Inversion ; waveforms ; attenuation ; Green's function ; representation theorem ; dual conditions ; reciprocity theorem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract To account for elastic and attenuating effects in the elastic wave equation, the stress-strain relationship can be defined through a general, anisotropic, causal relaxation functionψ ijkl (x, τ). Then, the wave equation operator is not necessarily symmetric (‘self-adjoint’), but the reciprocity property is still satisfied. The representation theorem contains a term proportional to the history of strain. The dual problem consists of solving the wave equation withfinal time conditions and an anti-causal relaxation function. The problem of interpretation of seismic waveforms can be set as the nonlinear inverse problem of estimating the matter density ϱ(x) and all the functionsψ ijkl (x, τ). This inverse problem can be solved using iterative gradient methods, each iteration consisting of the propagation of the actual source in the current medium, with causal attenuation, the propagation of the residuals—acting as if they were sources—backwards in time, with anti-causal attenuation, and the correlation of the two wavefields thus obtained.
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  • 53
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    Biology and fertility of soils 7 (1988), S. 67-70 
    ISSN: 1432-0789
    Keywords: Root activity ; Soil C mineralization ; 14C-labelled plant material ; Decomposition stages ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two different soils were amended with 14C-labelled plant material and incubated under controlled laboratory conditions for 2 years. Half the samples were cropped with wheat (Triticum aestivum) 10 times in succession. At flowering, the wheat was harvested and the roots removed from the soil, and a new crop was started. Thus, the soil was continuously occupied by predominantly active root systems. The remaining samples were maintained without plants under the same conditions. The aim of the experiment was to study the effects of active roots on C-mineralization rates during different stages of decomposition and during long-term incubation. During the first 200 days, corresponding to the active decomposition stages, the roots weakly reduced 14C mineralization. With a lower level of decomposition, when more than 60% of the initial 14C was mineralized and when the available nutrients were markedly exhausted by plant uptake, the roots stimulated 14C mineralization.[/ p]
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  • 54
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    Biology and fertility of soils 7 (1988), S. 71-78 
    ISSN: 1432-0789
    Keywords: Root activity ; Rhizosphere ; C metabolism ; Microbial biomass ; Microbial activity ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two different soils were amended with 14C-labelled plant material and incubated under controlled laboratory conditions for 2 years. Half the samples were cropped with wheat (Triticum aestivum) 10 times in succession. At flowering, the wheat was harvested and the old roots removed from the soil, so that the soil was continuously occupied by predominantly active root systems. The remaining samples were maintained without plants under the same conditions. During the initial stages of high microbial activity, due to decomposition of the labile compounds, the size of the total microbial biomass was comparable for both treatments, and the metabolic quotient (qCO2-C = mg CO2-C·mg−1 Biomass C·h−1) was increased by the plants. During the subsequent low-activity decomposition stages, after the labile compounds had been progressively mineralized, the biomass was multiplied by a factor of 2–4 in the presence of plants compared to the bare soils. Nevertheless, qCO2-C tended to reach similar low values with both treatments. The 14C-labelled biomass was reduced by the presence of roots and qCO2-14C was increased. The significance of these results obtained from a model experiment is discussed in terms of (1) the variation in the substrate originating from the roots and controlled by the plant physiology, (2) nutrient availability for plants and microorganisms, (3) soil biotic capacities and (4) increased microbial turnover rates induced by the roots.
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  • 55
    ISSN: 1432-0789
    Keywords: Biopores ; Root growth ; Transpiration ; Simulations ; Mechanical impedance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The use of vertical biopores by wheat (Triticum aestivum) seminal roots for easy access to the subsoil and the consequences for plant water supply and yield has been investigated by computer simulation. Parameters included were: biopore density and diameter, depth of cultivation and strength of the subsoil — all under a wide range of seasonal weather conditions. The model predicts that biopores add significantly to root penetration at depth, even at a density of 0.1% v/v of small, vertical pores, while 1.5% to 2.0% v/v can ensure maximum root penetration. When the growing season is shorter a larger number of biopores is needed to ensure timely root penetration to depth. With shallow tillage, biopores occur closer to the soil surface, and their importance is increased. Deeper root penetration invariably gives greater water uptake and transpiration, but may have a negative effect on grain yield, especially under the driest climatic conditions. An increase in early water use may result in less soil water being available during the grain-filling period. The effect of biopores on plant transpiration varies from year to year, depending on the amount of rain and its distribution in time, and on the amount of soil water stored at time of sowing.
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  • 56
    ISSN: 1432-0983
    Keywords: Ribosomal protein ; Immunological homology ; Yeast ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Polyclonal antibodies raised against ribosomal protein (r-protein) L2 of Schizosaccharomyces pombe were used to check for cross-reaktions with total r-proteins of rat liver. Using this procedure, the rat liver r-proteins, L4 and L24, were identified as being immunologically related to yeast L2. In addtional, homologies between rat liver L4 and L24 were detected. The possible implications for the regulation of r-protein synthesis are discussed.
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  • 57
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; tRNA genes ; Gene duplication ; Inversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined the DNA sequences of regions involved in two of the three inversions known to have occurred during the evolution of wheat chloroplast DNA. This establishes the extent of the second largest of the three inversions. Examination of these sequences suggests that although short repeated sequences are present, the endpoints of the second and third inversions are not associated with repeated sequences as long as those associated with the first inversion. However the endpoints of all three inversions are all adjacent to at least one tRNA gene, and there is evidence that three of the tRNA genes have been subjected to partial duplication, possibly at the time of inversion. This suggests that tRNA genes might be involved with rearrangements of chloroplast DNA, as has also been postulated for mitochondrial DNA.
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  • 58
    ISSN: 1432-0983
    Keywords: Chloroplast genome ; Inversion ; Transposon ; Spinach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 7,022 by BamHI-EcoRI fragment, located in the inverted repeat of spinach chloroplast, has been sequenced. It contains a 2131 codon open reading frame (ORF) homologous to both tobacco ORFs 581 and 1708, and to Marchantia ORF 2136. Relative to the Marchantia chloroplast genome, spinach ORF 2131 is located at the end of a large inversion; the other end point is close to trnL, the position of which is the same in Marchantia, tobacco and spinach. In Marchantia, two 8 by direct repeats flanking two 10 by indirect repeats are present near the end points of the inversion. These repeats may result from a transposon-mediated insertion which would have facilitated the subsequent inversion. From a comparison of the gene organization of the spinach, tobacco, and Marchantia genomes in this region, we propose a step-wise process to explain the expansion of the inverted repeat from a Marchantia-like genome to the spinach/tobacco genome.
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  • 59
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Plant mitochondrial DNA ; Ribosomal genes ; Sequence rearrangements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of the wheat mitochondrial 26S ribosomal RNA gene and flanking regions was determined and compared with mitochondrial 26S rRNA genes from maize and Oenothera. All three genes exhibit a high degree of homology except within two variable regions. When the plant mitochondrial 26S rRNA genes are compared with Escherichia coli 23S rRNA and chloroplast 23S and 4.5S rRNA genes, a third variable region is apparent close to the 3′ end of the gene. The 5′ and 3′ ends of the wheat mitochondrial gene were determined by S1 nuclease mapping. Computer analysis of the wheat mitochondrial gene revealed several small sequences present either in the 5′ region of the 26S rRNA gene or in the 18S rRNA gene.
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  • 60
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Genomic variability ; Mitochondrial DNA ; Somatic tissue culture ; Regeneration ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryogenic and non-embryogenic long-term callus cultures of hexaploid wheat exhibit differences in the organization of their mitochondrial genome. Embryogenic and non-embryogenic fractions of callus cultures initiated from immature embryos of the wheat cultivar “Chinese Spring” have been isolated and subsequently subcultured. DNA-DNA hybridization experiments using labelled cloned wheat mitochondrial DNA fragments have shown that the mitochondrial DNA organization of embryogenic subcultures derived from embryogenic parts of “Chinese Spring” calli is closely related to that of the initial “Chinese Spring” calli, while non-embryogenic subcultures derived from non-embryogenic fragments of “Chinese Spring” calli exhibit a mitochondrial DNA organization similar to that found in non-embryogenic calli derived from cultivar “Aquila”. In addition, somatic tissue cultures initiated from three other non-embryogenic wheat cultivars (“Talent”, “Thésée” and “Capitole”) display mitochondrial DNA arrangements similar to those found in cultivar “Aquila”. These results strongly suggest that, in wheat callus cultures, a particular mitochondrial genome organization is correlated with the ability of cultured cells to regenerate whole plants.
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  • 61
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    Journal of molecular evolution 28 (1988), S. 87-97 
    ISSN: 1432-1432
    Keywords: Genome evolution ; Rearrangement ; Inversion ; Brassica ; Base substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined the tempo and mode of mitochondrial DNA (mtDNA) evolution in six species of crucifers from two genera,Brassica andRaphanus. The six mtDNAs have undergone numerous internal rearrangements and therefore differ dramatically with respect to the sizes of their subgenomic circular chromosomes. Between 3 and 14 inversions must be postulated to account for the structural differences found between any two species. In contrast, these mtDNAs are extremely similar in primary sequence, differing at only 1–8 out of every 1000 bp. The point mutation rate in these plant mtDNAs is roughly 4 times slower than in land plant chloroplast DNA (cpDNA) and 100 times slower than in animal mtDNA. Conversely, the rate of rearrangements is extraordinarily faster in plant mtDNA than in cpDNA and animal mtDNA.
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  • 62
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    International journal of biometeorology 32 (1988), S. 17-20 
    ISSN: 1432-1254
    Keywords: Epilepsy ; Electromagnetic fields ; Rat ; Audiogenic seizure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract In order to study the possible association between epileptic seizures and natural electromagnetic fields, 32 female audiogenic seizure (AGS)-susceptible rats were exposed to simulated 10 kHz and 28 kHz atmospherics and to a sinusoidally oscillating magnetic field with a frequency of 100 Hz and field strength of 1 A/m. After the electromagnetic exposure, seizures were induced in the rats with a sound stimulus. The severity of the seizure was determined on an ordinal scale, the audiogenic response score (ARS). The time from the beginning of the sound stimulus to the onset of the seizure (seizure latency) and the duration of the convulsion was measured. No differences from the control experiments were found in the experiments with simulated atmospherics, but the 100 Hz magnetic field increased the seizure latency by about 13% (P〈0.02). The results do not support the hypothesis that natural atmospheric electromagnetic signals could affect the onset of epileptic seizures, but they suggest that AGS-susceptible rats may be a useful model for studying the biological effects of electromagnetic fields.
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  • 63
    ISSN: 1432-1254
    Keywords: Atmospherics ; Carrageenan inflammation ; Rat ; Susceptibility ; Correlations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract Between the mean daily density of 28 kHz atmospherics and the onset of epileptic fits there is a highly significant correlation coefficient (r) of 0.30; there is a negative coefficient of −0.20 between the fits and the mean daily density of 10 kHz atmospherics. The onset of heart infarction is correlated with 28 kHz atmospherics (r=0.15). Furthermore, we have discovered that sudden deafness is also correlated with certain configurations of atmospherics. In this paper we report the following correlation coefficients between the inflammatory reaction of rats to a carrageenan injection (rci) into a hind paw and the mean daily pulse rate of atmospherics of the same day:r=0.49 for the 8 kHz atmospherics (P〈0.02) andr=0.44 for the 10 kHz atmospherics (P〈0.04). The correlations between rci reaction and other atmospherics (12 and 28 kHz) are smaller and not significant. By the method of multiple linear regression we found a multipleR=0.54 between rci reaction and the 8 and 10 kHz atmospherics (the regression function for the rci reaction is 0.15+0.004×8 kHz+0.002×10 kHz,P〈0.05).
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  • 64
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    Sexual plant reproduction 1 (1988), S. 217-222 
    ISSN: 1432-2145
    Keywords: Triticum aestivum ; Cytology ; In vitroandrogenesis ; Fluorescent microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two winter wheat genotypes (‘Diószegi 200’ and ‘Mv 15’) were compared for their in vitro androgenic capacity. On average, the induction frequency of embryogenic structures was 71.7% in ‘Diószegi 200’ and only 4.3% in ‘Mv 15’. The haploid induction ability of the two genotypes differed considerably, with ‘Diószegi 200’ being much higher. The difference in the in vitro inductability of the microspores may result from genetic differences which are manifested in the survival rate of the microspores during the culture period and their adaptability to in vitro conditions. Special DNA fluorochrornes were suitable for studying the different pathways of in vitro androgenesis. Our data indicate that the repeated equal divisions of the microspore nucleus might lead to pollen embryo formation, and subsequent divisions of the vegetative portion of the pollen grain after the first asymmetric microspore mitosis can result in pollen callus formation.
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  • 65
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    Biochemical genetics 26 (1988), S. 287-301 
    ISSN: 1573-4927
    Keywords: shikimate dehydrogenase ; electrophoresis ; isoelectric focusing ; isozymest ; Triticeae ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Starch gel electrophoresis and polyacrylamide gel isoelectric focusing (IEF) were used to investigate the genetic control of Triticeae shikimate dehydrogenase-1 (SKDH-1). Studies of wheat-alien species chromosome addition lines established thatSkdh-1 ofHordeum vulgare cv. Betzes is located in chromosome 5H,Skdh-V1 ofDasypyrum villosum in 5V,Skdh-R1 ofSecale cereale cvs. Dakold and King II in 5R, andSkdh-S 11 ofTriticum longissimum in 5S1S. Also, the chromosomal locations of the genes that encode SKDH-1 inT. aestivum cv. Chinese Spring,T. umbellulatum, andS. cereale cv. Imperial, determined earlier using zone electrophoresis, were reconfirmed using IEF. Zone electrophoresis and IEF do not differ markedly in their ability to detect the expression of alienSkdh-1 genes in wheat-alien species chromosome addition lines. However, IEF may be superior to zone electrophoresis as a technique for detecting and analyzing SKDH-1 genetic variants within Triticeae species; among the species studied, IEF generally resolved two or more isozymes perSkdh-1 allele present, while zone electrophoresis resolved only one.
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  • 66
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    Plant molecular biology 11 (1988), S. 311-319 
    ISSN: 1573-5028
    Keywords: chloroplast gene ; iron-sulphur protein ; psaC ; Pisum sativum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene for the 8 kDa subunit of photosystem I has been located in the small single copy region of wheat chloroplast DNA by coupled transcription-translation of cloned fragments of DNA and by DNA sequence analysis. The pea gene for this subunit was located in pea chloroplast DNA by using the wheat gene as a probe. The location was confirmed by immunoprecipitation of the products of coupled transcription-translation of cloned DNA with antiserum raised against the small subunits of pea photosystem I and by DNA sequence analysis. The deduced amino acid sequences of the pea and wheat proteins are identical in seventy-six out of the eighty-one amino acid residues. There are nine conserved cysteine residues, eight of which are arranged in the primary structure in a similar way to those in bacterial ferredoxins containing two 4Fe-4S centres, suggesting that the polypeptide binds iron sulphur centres A and B of photosystem I.
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    Theoretical and applied genetics 75 (1988), S. 237-243 
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Hordeum spontaneum ; Spontaneous chromosomal rearrangement ; Reciprocal translocation ; Inversion ; Giemsa C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four of 1,240 cultivated barley lines collected from different regions of the world and 3 of 120 lines of wild barley, Hordeum spontaneum C. Koch, carry spontaneous reciprocal translocations. Break-point positions and rearrangements in the interchanged chromosomes have been examined by both test crosses and Giemsa banding techniques. The four translocation lines in cultivated barley were all of Ethiopian origin and have the same translocation involving chromosomes 2 and 4. The breakpoints are at the centromeres of both chromosomes, resulting in interchanged chromosomes 2S+4S and 2L+4L (S=short arm, L=long arm). A wild barley line, Spont.II, also has translocated chromosomes 2 and 4 which are broken at the centromeres. The resultant chromosomes are, however, 2S+4L and 2L+4S. Another wild barley line, Spont.S-4, has interchanged chromosomes with breakpoints in the short arm of chromosome 3 and the long arm of chromosome 7. In addition, this line has a paracentric inversion in the short arm of chromosome 7 that includes a part of nucleolar constriction, resulting in two tandemly arranged nucleolar constrictions. The third wild barley line, Spont.S-7, has interchanged chromosomes with breakpoints in the long arms of both chromosomes 3 and 6. The translocated chromosome 3 is metacentric and the translocated chromosome 6 has a long arm similar in length to the long arm of chromosome 7.
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    Cell & tissue research 252 (1988), S. 57-66 
    ISSN: 1432-0878
    Keywords: Heart ; Endothelium ; Tracer studies ; Junctional structures ; Permeability ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The isolated perfused heart model was used to examine the structure of rat cardiac capillaries and their permeability to macromolecules of various sizes. Haemoglobin (diameter 6.4 nm) and catalase (10.4 nm) did not cross the endothelium but remained on the luminal side. Cytochrome C (3 nm) and horseradish peroxidase (6 nm) both crossed the endothelium to the subendothelial space and filled the caveolae on the abluminal side as well as the entire length of the lateral intercellular spaces. The membranes of the endothelial cells are separated by an intercellular gap of mean width 18.2 nm. At one or more zonular regions within each lateral intercellular space the two membranes approach each other more closely and frequently appear to fuse. However, tilting the specimen shows that, in these regions, there is a gap of mean width 5.4 nm (in lanthanum- and tannic acid-treated tissue, 3.8 nm in ferrocyanide-treated tissue) between the membranes. We conclude that these narrow regions sieve macromolecules on the basis of size although other factors may determine their permeability properties.
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  • 69
    ISSN: 1432-0878
    Keywords: Uterus ; Intraepithelial lymphocytes ; Estrous cycle ; Early pregnancy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The number of intraepithelial lymphocytes (IEL) in the luminal and glandular epithelium of the uterus of virgin rats was analysed in diestrus, proestrus and estrus, and in nulliparous rats on days 5, 7 and 9 of pregnancy. IEL number was calculated either with respect to the number of epithelial cells or to the length of epithelium section. It was found that in diestrus, the number of IEL was, on average, 3.7 per 100 luminal epithelial cells or 6.7 per 1 mm of epithelium section, whereas in proestrus, it decreased to 0.9 and 1.2 IEL, respectively. On day 5 of pregnancy (before implantation) the number of IEL decreased further to 0.45 per 100 luminal epithelial cells or 0.9 per 1 mm of epithelium. On days 7 and 9 of pregnancy, IEL number further decreased in implantation sites, whereas in interimplantation sites it remained at the level calculated for day 5 of pregnancy. The population of uterine IEL consisted of small (82–99%) and large (1–18%) lymphocytes. In all stages of the estrous cycle, IEL occurred with a frequency of 68–87% in the basal region, 8–20% in the middle region and 4–12% in the apical region of the luminal epithelium width.
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  • 70
    ISSN: 1432-0878
    Keywords: Retinal pigment epithelium ; Cytoskeleton ; Cytokeratins ; Vimentin ; Desmosomes ; Anura (Rana ridibunda, Xenopus laevis) ; Chicken ; Bovine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In vertebrate tissue development a given cell differentiation pathway is usually associated with a pattern of expression of a specific set of cytoskeletal proteins, including different intermediate filament (IF) and junctional proteins, which is identical in diverse species. The retinal pigment epithelium (RPE) is a layer of polar cells that have very similar morphological features and practically identical functions in different vertebrate species. However, in biochemical and immunolocalization studies of the cytoskeletal proteins of these cells we have noted remarkable interspecies differences. While chicken RPE cells contain only IFs of the vimentin type and do not possess desmosomes and desmosomal proteins RPE cells of diverse amphibian (Rana ridibunda, Xenopus laevis) and mammalian (rat, guinea pig, rabbit, cow, human) species express cytokeratins 8 and 18 either as their sole IF proteins, or together with vimentin IFs as in guinea pig and a certain subpopulation of bovine RPE cells. Plakoglobin, a plaque protein common to desmosomes and the zonula adhaerens exists in RPE cells of all species, whereas desmoplakin and desmoglein have been identified only in RPE desmosomes of frogs and cows, including bovine RPE cell cultures in which cytokeratins have disappeared and vimentin IFs are the only IFs present. These challenging findings show that neither cytokeratin IFs nor desmosomes are necessary for the establishment and function of a polar epithelial cell layer and that the same basic cellular architecture can be achieved by different programs of expression of cytoskeletal proteins. The differences in the composition of the RPE cytoskeleton further indicate that, at least in this tissue, a specific program of expression of IF and desmosomal proteins is not related to the functions of the RPE cell, which are very similar in the various species.
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  • 71
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    Cell & tissue research 254 (1988), S. 251-253 
    ISSN: 1432-0878
    Keywords: Space flight ; Skeletal muscle ; Collagen ; Protein ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Biochemical determinations of non-collagenous protein and hydroxyproline were made on rat skeletal muscles following 7 days of space flight aboard the NASA space shuttle mission SL-3. Relative to ground-based controls, the wet weight of each experimental muscle was significantly reduced. This was concomitant with a reduction in noncollagenous protein in the muscles. Protein concentration, however, was reduced only in slow-twitch muscles. The effect of space flight on the concentration and hydroxyproline content was different among the muscles. As a result, the loss of muscle mass in some muscles was the consequence of a reduction in both collagenous and non-collagenous proteins, while in others it was primarily the result of a non-collagenous protein loss.
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  • 72
    ISSN: 1432-0878
    Keywords: Pineal complex ; Pinealocytes, receptor line ; Subcommissural organ ; Immunocytochemistry ; Protein secretion ; Neuroendocrine system Geotria australis (Cyclostomata) ; Onkorhynchus kisutch (Teleostei) ; Eupsophus roseus (Anura) ; Heloderma suspectum, Varanus monitor (Lacertilia) ; Domestic fowl ; Rat ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By means of light-microscopic immunocyto-chemistry two polyclonal antibodies (AFRU, ASO; see p. 470) directed against secretory glycoproteins of the subcom-missural organ were shown to cross-react with cells in the pineal organ of lamprey larvae, coho salmon, a toad, two species of lizards, domestic fowl, albino rat and bovine (taxonomic details, see below). The AFRU-immunoreactive cells were identified as pinealocytes of the receptor line (pineal photoreceptors, modified photoreceptors or classical pinealocytes, respectively) either due to their characteristic structural features or by combining AFRU-immunoreaction with S-antigen and opsin immunocytochemistry in the same or adjacent sections. Depending on the species, AFRU- or ASO-immunoreactions were found in the entire perikaryon, inner segments, perinuclear area, and in basal processes facing capillaries or the basal lamina. In most cases, only certain populations of pinealocytes were immunolabeled; these cells were arranged in a peculiar topographical pattern. In lamprey larvae, immunoreactive pinealocytes were observed only in the pineal organ, but not in the parapineal organ. In coho salmon, the immunoreaction occurred in S-antigen-positive pinealocytes of the pineal end-vesicle, but was absent from S-antigen-immunoreactive pinealocytes of the stalk region. In the rat, AFRU-immunoreaction was restricted to S-antigen-immunoreactive pinealocytes found in the deep portion of the pineal organ and the habenular region. These findings support the concept that several types of pinealocytes exist, which differ in their molecular, biochemical and functional features. They also indicate the possibility that the AFRU- and ASO-immunoreactive material found in certain pinealocytes might represent a proteinaceous or peptidic compound, which is synthesized and released from a specialized type of pinealocyte in a hormone-like fashion. This cell type may share functional characteristics with peptidergic neurons or paraneurons.
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  • 73
    ISSN: 1432-0878
    Keywords: Perivascular space ; Cerebrospinal fluid compartments ; Dura mater ; Pacchionian granulations ; Rat ; Cebus apella, Callitrix jacchus (Primates)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary At the sites where a vein penetrates through the dura mater, two aspects deserve particular attention: (i) The delineation of the perivascular cleft, a space belonging to the interstitial cerebrospinal fluid (CSF) compartment, toward the interior hemal milieu of the dura mater. (ii) The relationship between the perivascular arachnoid layer and the subdural neurothelium at the point of vascular penetration. These problems were investigated in the rat and in two species of New-World monkeys (Cebus apella, Callitrix jacchus). Concerning the first aspect, tight appositions of meningeal cells to the vessel wall, the basal lamina of which is widened and enriched with microfibrils, prevent communication between the interstitial CSF in the perivascular cleft and the hemal milieu in the dura mater. With reference to the second aspect, the perivascular arachnoid cells are transformed into neurothelial cells at the point where they become exposed to the hemal milieu of the dura mater and subsequently continuous with the subdural neurothelium. Leptomeningeal protrusions encompassing outer CSF space can penetrate into the dura mater. These protrusions may expand and branch repeatedly, forming along the wall of the durai sinus Pacchionian granulations. At these sites, however, the structural integrity of the sinus wall and the Pacchionian granulation is not lost. Numerous vesiculations not only in the sinus and vascular walls, but also in the cellular arrays of the Pacchionian granulations or paravascular leptomeningeal protrusions indicate mechanisms of transcellular fluid transport. Moreover, the texture of the leptomeningeal protrusions favors an additional function of these structures as a “volume” buffer.
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  • 74
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    Cell & tissue research 251 (1988), S. 171-181 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Basal lamina ; Laminin ; Anterior pituitary gland ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To determine whether circulating antibodies against laminin can bind in vivo to basement membranes within endocrine glands, affinity-purified sheep or rabbit anti-laminin IgG was intravenously injected into rats. One to five hours after injection, anti-laminin IgG was bound to all basement membranes of adrenal and anterior pituitary glands of mature as well as 2-day-old newborn rats as shown by immunofluorescence microscopy. After the injection of anti-laminin conjugated directly to horseradish peroxidase (HRP), HRP reaction product was also present throughout adrenal and pituitary basement membranes in mature and immature glands 1–5 h post-injection. Ultrathin Lowicryl sections from rats that received unconjugated rabbit anti-laminin IgG 1 h prior to fixation with paraformaldehyde were labeled directly with anti-rabbit IgG-colloidal gold. In these cases, gold also bound specifically over the lamina densa and lamina rara. When adrenal or pituitary glands from mature rats were examined by immunofluorescence 1 week after the injection of sheep anti-laminin IgG, the patterns and amounts of bound sheep IgG were indistinguishable from those observed 1 h after injection. In contrast, significantly less fluorescence was present in glands from 7-day-old rat pups that had received anti-laminin IgG 5 days earlier. In addition, when anti-laminin IgG-HRP was injected into newborns and glands were fixed 5 days later, lengths of labeled endothelial and epithelial basement membranes were often interspersed with unlabeled lengths in zones of cellular proliferation in the outer adrenal cortex and throughout the pituitary gland. These results indicated that unlabeled basement membranes in these regions were probably assembled after the injection of anti-laminin IgG, which would also explain diminished labeling of basement membranes in these animals. Despite the continued presence of heterologous anti-laminin IgG within endocrine basement membranes, however, rat IgG, rat C3, inflammatory cells, or histologic abnormalities were observed in neither newborn nor adult glands under the conditions examined here. Sections from rats injected with control IgG or control IgG-HRP were entirely negative by immunofluorescence, immunoperoxidase, and immunogold techniques. We therefore conclude that (1) apparently large amounts of circulating anti-laminin IgG can bind to adrenal and pituitary basement membranes, and (2) at least some of these basement membranes are assembled during development by progressive splicing of newly synthesized matrix into that already present.
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  • 75
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    Cell & tissue research 252 (1988), S. 181-190 
    ISSN: 1432-0878
    Keywords: Embryonic rat brain ; Cell culture ; Development ; NILE-glycoprotein ; Neurons ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fetal rat cerebral cells underwent neuronal differentiation in culture. This process was accompanied by distinct changes in the cellular glycoprotein pattern. The incorporation of [3H]-fucose into two proteins of apparent molecular weights of 30000 and 60000 daltons was significantly decreased and specific developmental changes were observed in a group of glycoproteins with high molecular weights (150000–250000 dalton). By means of indirect immunoprecipitation one of them was identified as NILE gp (nerve growth factor-inducible large external) glycoprotein (200000 dalton), a marker of central and peripheral neurons. Its developmental expression on neurons of dissociated rat cerebral cultures was studied using the indirect immunofluorescence technique and compared to the fluorescent-labeling pattern of other neuronal markers. Neurons expressing NILE gp were detected as early as after one day in culture. No preferential staining of neuntes versus cell bodies was observed. Two classes of NILE gp-positive cells were identified. One group consisted of a rounded cell-type, whereas the other group was represented by larger, more spindle-shaped neurons with a limited number of neuritic processes. In most cases one of these neuritic processes was preferentially labeled. Astroglia cells, as identified by immunolabeling with antisera against the glial acidic fibrillary protein, were observed to develop and mature after the first week in culture. NILE-positive neurons were found to be positioned in close association with glial cell processes.
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  • 76
    ISSN: 1432-0878
    Keywords: Endocytosis ; Absorptive cells ; Ileum ; Intestine, small ; Apical tubules ; Membrane recycling ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ileal absorptive cells of suckling rats exhibit high levels of endocytic activity being engaged in nonselective uptake of macromolecules from the intestinal lumen. The apical cytoplasm usually contains an extensive network of small, membrane-limited tubules (apical tubules: AT), in addition to newly formed endocytic vesicles and large endocytic vacuoles. To determine whether the AT are directly involved in the endocytic process by carrying the tracer into the cell, we have analysed movements of the apical cell membrane of the ileal absorptive cells by using a membrane-bound tracer (horseradish peroxidase-labelled cancanavalin-A: Con-A HRP). The ileal absorptive cells were exposed in vitro to Con-A HRP for 10 min at 4° C, incubated for different times in Con-A free medium at 37° C, and prepared for electron microscopy. After 1 min incubation at 37° C, invaginations of the apical cell membrane, including coated pits, and endocytic vesicles were labelled with HRP-reaction product, whereas the AT and large endocytic vacuoles were negative. After 2.5 min, almost all the large endocytic vacuoles were labelled with reaction product, which was seen in their vacuolar lumen and along the luminal surface of their limiting membrane. A few AT with reaction product were seen in the apical cytoplasm; they were in frequent connection with the reaction-positive large endocytic vacuoles. With increasing incubation time, the number of the labelled AT increased. Thus, after 15 min at 37° C, the apical cytoplasm was fully occupied by the reaction-positive AT. The ends of these AT were often continuous with small spherical coated vesicles. No reaction product was detected in the Golgi complex at any time after incubation. These observations indicate that the AT located in the apical cytoplasm probably originate by budding off from the large endocytic vacuoles, rather than being involved in the process of endocytosis.
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  • 77
    ISSN: 1432-0878
    Keywords: Periodontal ligament ; Incisor ; Neurofilament protein ; S-100 protein ; Immunohistochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the nerve fibers and their terminations differed markedly from those in molars. NFP-positive, thick nerve bundles entered the lingual periodontal ligament through slits located in the mid-region of the alveolar socket, and immediately formed numerous Ruffini-like corpuscles. In the labial periodontal ligament, all of the NFP-immunoreactive nerve fibers terminated in free endings. The restricted location of the stretch receptor, Ruffini-like corpuscle, in the lingual periodontal ligament appears to be an essential element, because this region is regularly extended during mastication. The nervous elements were restricted to the alveolar half of the periodontal ligament in every region; they avoided the dental half of the periodontal ligament, which presumably moves continuously with the tooth. Pulpal nerve fibers in incisors also showed a characteristic distribution different from those in molars; individual nerve fibers with beaded structures ran in the center of the pulp toward the incisai edge, and did not form the subodontoblastic nerve plexus of Raschkow. Immunostaining for S-100 protein revealed a distribution pattern of nervous elements similar to that for NFP, suggesting that the nerves supplying the periodontal ligament and dental pulp were mostly covered by a Schwann sheath.
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  • 78
    ISSN: 1432-0878
    Keywords: Posterior pituitary ; Immunocytochemistry ; Anti-GABA ; GABA-transaminase ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antibody against gamma-aminobutyric acid (GABA) was used to identify GABAergic elements immunocytochemically in the rat posterior pituitary. In order to increase the intracellular concentration of GABA, rats were treated with the GABA-transaminase inhibitor gamma-vinyl-GABA (GVG). Light-microscopic observations of Vibratome and semithin sections revealed the presence of numerous immunoreactive nerve fibers throughout the neural lobe; the mean number and length of these fibers increased by 90% after GVG treatment. Electron microscopy demonstrated the immunostained axons to be of small diameter. The reaction product was confined to small vesicles. No immunostaining occurred in pituicytes. The richness of the GABAergic innervation of the neural lobe contrasts with previous reports using antibodies against glutamate decarboxylase and supports the idea that GABA participates in the presynaptic control of neurosecretion.
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  • 79
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    Cell & tissue research 251 (1988), S. 65-70 
    ISSN: 1432-0878
    Keywords: Adipose tissue ; Cell proliferation ; Cell differentiation ; Histochemistry ; Swine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cells in fetal adipose tissue and cells in vitro are characterized by rapid proliferation. Serum factors have been shown to be important for the rapid proliferation of cells in vitro. The present experiment was performed to determine if neuroendocrine regulatory mechanisms of the fetus can influence the actions of serum factors on preadipocyte proliferation and differentiation in vitro. Sera were obtained from decapitated fetal pigs and intact littermates during gestation. Sera were tested for their effects on primary cultures of preadipocytes and stromalvascular cells derived from inguinal adipose tissue of young Sprague-Dawley rats. Coverslip cultures were used for histochemical analysis of enzymes after 12 days of incubation with test media. Analysis of growth curves produced from sequential [3H]-thymidine labeling indicated that fetal age influences rates of proliferation. Sera from decapitated fetal pigs specifically reduced the number of proliferating preadipocytes in culture. Sera from decapitated fetal pigs induced a minimum of 50% less differentiation of sn-glycerol-3-phosphate dehydrogenase activity than sera from intact pigs at all fetal ages. Histochemical staining for enzymes of differentiating preadipocytes was also reduced in cultures incubated with sera from decapitated fetal pigs in comparison to sera from intact pigs. The present study has demonstrated that the in vivo effect of decapitation on fetal adipose tissue development is a consequence of alterations in systemic factors present in serum in response to removal of central regulation by the hypothalamic-pituitary axis.
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  • 80
    ISSN: 1432-0878
    Keywords: Monoclonal antibody ; Blood-brain barrier ; Cerebral pericytes ; Transporting epithelia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A monoclonal antibody that recognizes a 140 kDa peripheral plasma membrane protein in pericytes of nervous tissues of the rat is described. Microvessels of brain cortex and perineurium of peripheral nerves are shown to react positively to this antibody. The antigen is absent in brain regions that lack a blood-brain barrier, i.e., choroid plexuses and area postrema. Antigen expression starts as early as day 18 of embryonic development. By means of immuno-electron microscopy the 140 kDa antigen was detected as clusters along the entire circumference of cerebral pericytes. The same antigenic determinant is also expressed in apical domains of plasma membranes of a variety of transporting epithelia, such as hepatocytes, enterocytes of the small intestine, and epithelial cells of proximal tubules of the kidney. We postulate the 140 kDa protein as being a constituent of the pericytes involved in regulative functions of the blood-brain barrier.
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  • 81
    ISSN: 1432-0878
    Keywords: Ciliary epithelium ; Orthogonal arrays of particles ; Filipin ; Freeze-fracture ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It has been suggested that orthogonal arrays of particles may increase the rigidity of plasma membrane, as does cholesterol. Therefore, using freeze-fractured non- pigmented ciliary epithelium, the distribution of such arrays was compared to the distribution of membrane deformations induced by the sterol-probes filipin and digitonin in different domains of the basolateral plasma membrane. The distribution of orthogonal arrays of particles was homogeneous between different regions of the basolateral membrane of the non-pigmented ciliary epithelium, while the number of filipin-induced alterations was nearly 4 times higher in the membrane domains not in contact with the basal lamina than in domains in contact with it. Contrary to the homogeneous distribution of arrays, digitonin-induced deformations also differed markedly in these two basolateral membrane domains. Considering that a marked positive response to sterol probes implies a high sterol content, we conclude that orthogonal arrays of particles can occur in plasma membrane regions well-provided with cholesterol and not in direct contact with the basal lamina. Other possible roles of these arrays are discussed.
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  • 82
    ISSN: 1432-0878
    Keywords: Bone phosphoprotein ; Osteopontin ; Kidney ; Inner ear ; Trigeminal nerve ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous immunohistochemical data have shown that the 44-kDal bone phosphoprotein (44K BPP, also called sialoprotein I or oestopontin) recently isolated in our laboratory was synthesized by osteoblasts and osteocytes and was expressed early during differentiation of boneforming cells. We report here the presence of 44K BPP antigenicity at certain ectopic sites, namely, the proximal-convoluted tubule of the kidney, neurons, sensory and secretory cells in the internal ear. To insure specificity and reproducibility, different immunohistochemical methods were used and affinity-purified antibodies against two separate preparations of pure 44K BPP were tested. In the cells of the proximal-convoluted tubule, 44K BPP immunoreactivity was observed within apical endocytotic vacuoles and within lysosomes. This staining thus correlates with the degradation of the 44K BPP epitope which we previously demonstrated to occur in serum. On the other hand, in the neurons of the acoustic ganglion and the sensory cells of the macula, 44K BPP immunoreactivity was associated with the Golgi apparatus indicating synthesis and secretion by these cells. The finding that the 44K BPP (or a structurally related molecule) is synthesized by neurons and neuroepithelial cells deserves further investigation with respect to a possible embryologie relationship between neuroectodermal cells and the precursors of some bone forming-cells of the skull.
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  • 83
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    Cell & tissue research 251 (1988), S. 117-121 
    ISSN: 1432-0878
    Keywords: Collagen ; Submucosa ; Intestine ; Scanning electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The three-dimensional architecture of the submucosal collagen fibres of the rat (3 weeks old) small intestine was examined by scanning electron microscopy using a selective microdissection method. The main framework of the submucosa was composed of two arrays of collagen fibre bundles running diagonally around the intestinal wall, one set in a clockwise direction, the other counterclockwise. These fibre bundles were about 5 μm in diameter and were oriented at a range of angle ± 30°–50° to the longitudinal axis of the intestine. With the advantage of the SEM observation it was demonstrated that these fibres in different arrays did not constitute two separate layers but interwove to form a unified lattice sheet. An irregular network of fine collagen fibrils over the main framework was also seen. The significance of their arrangement is discussed with respect to the skeletal function of the submucosa in the intestine.
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  • 84
    ISSN: 1432-0878
    Keywords: Adipocyte ; Primary culture ; Collagen ; Fibronectin ; Serum ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of collagenous substrata, fibronectin, and fetal bovine serum on the adhesion, proliferation, and adipogenesis of rat stromal-vascular cells are reported. There was no effect on initial stromal-vascular cell-attachment by fetal bovine serum or fibronectin. The number of cells attached to a hydrated collagen-gel was almost twice (P〈0.04) the number attached to dried collagen-gel or dried denatured collagen-gel. Total number of cells after 5 days in culture was similar among the collagenous substrata and among the treatments with or without fibronectin in the growth media. Total number of cells increased significantly (P〈0.02) with 10% FBS. Adipocytic formation was inhibited by hydrated collagen-gel (P〈0.02) compared to dried collagen-gel or dried, denatured collagenous substrata. An interaction occurred between dried, denatured gel and fetal bovine serum so that total formation of adipocytes increased by increasing the level of fetal bovine serum (P〈0.07). Adipocytic formation was inhibited by hydrated collagen-gel at all levels of fetal bovine serum. The percentage of cells that converted to adipocytes was significantly lower (P〈0.01) on hydrated collagen-gel compared to dried, denatured or dried collagen-gel. Percentage of conversion was not significantly different among levels of fetal bovine serum, although this percentage increased as fetal bovine serum level increased. Adipocytic conversion was not different between fibronectin-treated or untreated cells. Morphology of stromal vascular cells was similar on dried collagen and dried, denatured collagen-gel, but tended to remain bipolar on hydrated collagen-gel. These studies indicate that fetal bovine serum in combination with the extracellular matrix (dried, denatured collagen) increased the differentiation of rat stromal-vascular cells into adipocytes, and that hydrated collagen inhibited differentiation.
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  • 85
    ISSN: 1432-0878
    Keywords: Atrial natriuretic peptide ; Ventricular myocytes ; Atrial myocytes ; Cell culture ; Secretion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have demonstrated that atrial natriuretic peptide-like immunoreactivity is stored and secreted by ventricular and atrial myocytes in dissociated cell culture preparations from the heart of newborn rat. Culture preparations were maintained in either foetal calf serum-supplemented medium 199 or in hormone-supplemented, serum-free medium 199. The presence of atrial natriuretic peptidelike immunoreactivity in the cultured myocytes was demonstrated at both light-and electron-microscopical levels. Release of atrial natriuretic peptide-like immunoreactivity into the culture medium was measured by radioimmunoassay; molecular forms of the stored and secreted peptide were determined by gel column chromatography. The atrial natriuretic peptide-like immunoreactivity of cultured atrial and ventricular myocytes was concentrated in the perinuclear cytoplasm and was localised to electron-dense secretory granules. The number of immunoreactive ventricular myocytes and the intensity of their immunofluorescence changed with time in culture and was higher in cultures in foetal calf serum-supplemented medium than in serum-free medium. Gamma-atrial natriuretic peptide was stored and released by cultured atrial and ventricular myocytes, but was broken down to alpha-atrial natriuretic peptide in the growth medium. This process was foetal calf serum-independent, since it occurred in both the media used, indicating that cardiac myocytes in culture may release a factor that cleaves gamma-atrial natriuretic peptide to form alphaatrial natriuretic peptide.
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  • 86
    ISSN: 1432-0878
    Keywords: Enkephalin-nerves ; Transient appearance ; Submandibular gland ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A time course study with enkephalin(Enk)-like immunoreactivity has revealed that nerve fibers intensely immunoreactive for Enk-8 appeared transiently only during the postnatal week 2 and 4 within the acini as well as in the inter- and intralobular connective tissues of the submandibular gland of rats. At these stages numerous nerve fibers immunoreactive for tyrosine hydroxylase (TH) appeared also in the inter- and intralobular connective tissues and within the acini. Coincidently with these postnatal stages, abundant Enk-immunoreactive principal ganglion cells appeared in the superior cervical ganglion. These were not immunoreactive for neuropeptide tyrosine (NPY). A substantial number of Enk-immunoreactive ganglion cells were also present in the submandibular ganglia at these younger postnatal stages. Superior cervical ganglionectomy at these stages resulted in a marked decrease in number of the inter- and intralobular Enk-immunoreactive nerve fibers, a slight decrease in number of the intraacinar Enk-immunoreactive nerve fibers, and almost complete disappearance of intraglandular TH-immunoreactive nerve fibers. Immuno-electron-microscopic analysis revealed that Enk-immunoreactive nerve fibers in the submandibular gland were identified as electron-dense neuronal profiles enclosed by Schwann cells in the inter- and intralobular connective tissues and those directly apposed to secretory cells within the acini. They contained small clear vesicles mixed with some large granular vesicles. After postnatal week 6, no Enk-immunoreactive nerve fibers were detected in the submandibular gland, and no TH-immunoreactive nerve fibers were seen within the acini, while TH-immunoreactive nerve fibers remained numerous in the inter- and intralobular connective tissues. These findings indicate that both sympathetic and parasympathetic nerve fibers exhibit Enk-like immunoreactivity transiently during postnatal weeks 2 and 4. It is further indicated that the inter- and intralobular nerve fibers lose Enk-like immunoreactivity while the intraacinar fibers disappear at the adult stage.
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  • 87
    ISSN: 1432-0878
    Keywords: Gap junctions ; cAMP ; Theophylline ; Freeze-fracture ; Liver ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In view of the presumed involvement of gap junctions in the coordination of metabolic activities, the influence of cAMP as a regulatory signal of cell metabolism on gap junctions of hepatocytes has been examined. Male rats received two intraperitoneal doses of 10 mg dibutyryl cAMP/100 g body weight with a time interval of 2.5 h and were decapitated 2.5 h later. After this 5-h interval, analysis of freeze-fracture replicas of fixed liver tissue revealed an increase in the mean (± SEM) gap-junctional membrane portion on the lateral hepatocyte membranes from 0.049 + 0.003 (n = 66) in controls to 0.061 ± 0.003 (n = 70) in treated rats, while the configuration of the connexons appeared unaltered. This effect could not be reinforced by prior administration of aminophylline: the relative gapjunctional area is similarly extended from 0.054 ± 0.003 (n = 126) in the control group to 0.065 ± 0.004 (n = 105) in the experimental animals. Probing for the time course of the junctional response, a group of rats was sacrificed 3 h after the onset of treatment. Already within this time, the gapjunctional area is augmented from 0.042 ± 0.004 (n = 63) in the concurrent controls to 0.069 ± 0.006 (n = 42) in the treated rats. These statistically significant increases in area may suggest a stimulating effect of cAMP on gap junctions of hepatocytes in vivo.
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  • 88
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    Cell & tissue research 251 (1988), S. 523-529 
    ISSN: 1432-0878
    Keywords: Glial fibrillary acidic (GFA) protein ; S-100 protein ; Supportive cells ; Intestine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The non-neuronal, supportive cells of the enteric nerve plexus were investigated in the colon and rectum of adult and developing rats by means of immunohistochemistry, utilizing antisera against GFA protein and S-100 protein. Immunoreactivity to GFA protein was almost exclusively found in cells associated with the myenteric plexus and a small number of cells within the submucous ganglia. On the other hand, the use of S-100 protein antiserum resulted in the visualization of all supportive elements in the enteric nervous system. However, two types of supportive cells could be tentatively differentiated in the enteric nerve plexus during the second week of postnatal development, using GFA protein and S-100 protein antisera; GFA protein-positive cells were clearly discernible from S-100 protein-positive cells in terms of both the morphological profiles and immunohistochemical properties. It was assumed that at least two different types of supportive cells are contained in the enteric nerve plexus. We suggest that in the enteric nervous system the terms “glial cells” and “Schwann cells” should be employed to designate the supportive cells containing GFA and S-100 proteins, and cells containing S-100 protein, respectively. We discuss the possibility that glial cells are associated with the parasympathetic preganglionic fibres directly derived from the central nervous system, while Schwann cells originate from the neural crest.
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  • 89
    ISSN: 1432-0878
    Keywords: Calcium-binding protein ; Enteric nervous system ; Intestine ; Immunocytochemistry ; Guinea-pig ; Rat ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunoreactivity for vitamin D-dependent calcium-binding protein (CaBP) has been localized in nerve cell bodies and nerve fibres in the gastrointestinal tracts of guinea-pig, rat and man. CaBP immunoreactivity was found in a high proportion of nerve cell bodies of the myenteric plexus, particularly in the small intestine. It was also found in submucous neurons of the small and large intestines. Immunoreactive nerve fibres were numerous in the myenteric ganglia, and were also common in the submucous ganglia and in the intestinal mucosa. Immunoreactive fibres were rare in the circular and longitudinal muscle coats. In the myenteric ganglia of the guinea-pig small intestine the immunoreactivity is restricted to one class of nerve cell bodies, type-II neurons of Dogiel, which display calcium action potentials in their cell bodies. These neurons were also immunoreactive with antibodies to spot 35 protein, a calcium-binding protein from the cerebellum. From the distribution of their terminals and the electrophysiological properties of these neurons it is suggested they might be sensory neurons, or perhaps interneurons. The discovery of CaBP in restricted sub-groups of enteric neurons may provide an important key for the analysis of their functions.
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  • 90
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    Cell & tissue research 251 (1988), S. 275-280 
    ISSN: 1432-0878
    Keywords: Cerebral arteries ; Vasoactive intestinal polypeptide ; Vascular innervation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pathway of nerves with vasoactive intestinal polypeptide(VIP)-like immunoreactivity to the major cerebral arteries was studied in rats by means of the indirect immunofluorescent method. The fibers are densely distributed in the ethmoidal nerves and in the adventitia of both the external and internal ethmoidal arteries. Section of both ethmoidal nerves and external ethmoidal arteries before they enter the cranial cavity induced a marked reduction of VIP-like immunoreactive fibers in the walls of the vessels of the circle of Willis and its major branches. However, section of the external ethmoidal artery alone did not result in visible changes of the nerves around major cerebral arteries. The present study suggests that VIP-like immunoreactive fibers surrounding major cerebral arteries of the rat arise from fibers in the ethmoidal nerve showing immunoreactivity to VIP.
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  • 91
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    Cell & tissue research 251 (1988), S. 243-248 
    ISSN: 1432-0878
    Keywords: Thoracic duct ; Smooth muscle cell ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The three-dimensional cytoarchitecture and ultrastructure of the smooth muscle cells in the wall of the rat thoracic duct were investigated by scanning and transmission electron microscopy. The muscle layer basically consists of a single layer of circularly arranged cells. The smooth muscle cell is fusiform or ribbon-like in shape, as in veins or venules with a similar or smaller diameter. Connections by spinous processes are observed between adjacent muscle cells along their length. Spot-like membrane contacts frequently occur in areas where facing membranes are closely apposed. These are thought to be gap junctions and may be responsible for electrical coupling and mechanical attachment. Large invaginations arranged regularly in rows on the surface of the smooth muscle cells can be observed. These invaginations are closely associated with a flattened sarcoplasmic reticulum, and caveolae tend to open into the invaginations.
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  • 92
    ISSN: 1432-0878
    Keywords: GABA ; Glutamate decarboxylase ; GABA transaminase ; Exocrine pancreas ; Immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, l-glutamate decarboxylase (GAD) and gamma aminobutyrate-α-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar cell, GABA and its biosynthetic enzyme, GAD, were localized in zymogen granules. Quantitative analysis of the GABA immunoreactivity in the acinar cell revealed 1.7±0.5 gold particles/μm2 over the cytoplasm, 36.6±14.1 gold particles/ μm2 over the zymogen granules, and 2.9±2.1 gold particles /μm2 over the mitochondria. Quantitative analysis of the distribution of colloidal gold particles, representing glutamate decarboxylase immunoreactivity in the acinar cells, revealed 38.4±2.5 gold particles/μm2 over the zymogen granules, 4.7±1.1 gold particles/μm2 over the mitochondria and 6.3±0.5 gold particles/μm2 over the remainder of the cytoplasm. Substitution of normal sheep serum for the sheep anti-glutamate decarboxylase serum revealed a significant (p〈 0.001) decrease of the colloidal gold particle distribution over the zymogen granules and cytoplasmic compartments of the acini. Gamma aminobutyrate -α-ketoglutarate transaminase, the catabolic enzyme for GABA, was not detected in the mitochondria, zymogen granules, and cytoplasm of the acinar cell, suggesting that GABA is not catabolized within the acinar cell. Preabsorption and substitution controls resulted in an absence of labeling. These results suggest that GABA may act extracellularly and/or have a role within the zymogen granule in the exocrine pancreas.
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  • 93
    ISSN: 1432-0878
    Keywords: MPTP ; Rat ; Nigrostriatum ; Parkinsonism ; Immunocytochemistry ; Tyrosine hydroxylase ; Animal model ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several laboratories have reported that N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine causes damage to the nigral dopamine neurons of man, monkey, and mouse. Controversial data suggest that a rat model of Parkinsonism may be possible. Although loss of dopamine cells has not been detected in the rat brain, our immunocytochemical studies show that immunoreactive tyrosine hydroxylase, the rate-limiting enzyme which synthesizes dopamine, is significantly reduced in concentration, or its antigenicity altered, in substantia nigra/pars compacta as well as the caudate nucleus. Optical density measurements demonstrate the reduction or alteration of immunoreactive tyrosine hydroxylase in nigro-striatal neurons, indicating that axonal terminals, as well as parent perikarya, may be sensitive to the drug. After treatment, abnormal morphological remodelling may result in the affected neuronal processes, perhaps indicating sublethal toxicity, followed by slow recovery. Despite the lack of nigral cell death, it is proposed that the present data support the use of the rat as a model to investigate the early effects of Parkinsonism induced by this agent, and the biological mechanisms of cellular recovery.
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  • 94
    ISSN: 1432-0878
    Keywords: Pancreas, endocrine ; Stomach ; Intestine ; Immunohistochemistry ; Thyrotropin-releasing hormone (TRH) ; Somatostatin ; Avian pancreatic polypeptide ; Insulin ; Gastrin ; Rat ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By use of the indirect immunofluorescence technique, the cellular localization of thyrotropin-releasing hormone (TRH) was studied in the gastrointestinal tract of rats and guinea pigs of different ages. TRH-like immunoreactivity (LI) was observed in many pancreatic islet cells of young rats and guinea pigs but only in single cells of 6-month-old rats. In aged guinea pigs, a reduction in the number of TRH-positive cells was evident; however, numerous strongly fluorescent cells were still present. In the guinea pig, TRH-LI was in addition observed in gastrin cells in the stomach. TRH-positive nerve fibers occurred in the myenteric plexus of the oesophagus, stomach and intestine of the rat, and in the muscle layers of the guinea pig. These results suggest a functional role of TRH both as hormone and neuroactive compound in various portions and sites of the gastro-intestinal tract of the rat and guinea pig
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  • 95
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    Cell & tissue research 251 (1988), S. 205-214 
    ISSN: 1432-0878
    Keywords: Kupffer cells ; Granulocytes ; Ultrastructure ; Lipopolysaccharide ; Liver ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of Kupffer cells has been studied at various times after an intravenous injection of lipopolysaccharide of Salmonella abortus equii. The most prominent effects were: an increase in the number and dimensions of phagocytic vacuoles (often containing ingested LPS and neutrophilic granulocytes); mitochondrial damage, including disintegration of the matrix and cristae; an increase in the amount of dilated, lucent rough endoplasmic reticulum; presence of fat droplets in the cytoplasm. Five days after injection of lipopolysaccharide, the Kupffer cells had resumed their normal ultrastructure. Several minutes after injection of lipopolysaccharide, platelets adhered to the Kupffer and endothelial cells. Between one and six hours, neutrophilic granulocytes accumulated in the liver sinusoids. The resulting obstruction of the hepatic microcirculation most probably affected cellular ultrastructure by ischaemia. At three days, the number of Kupffer cells was doubled, and increased further at later time intervals.
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  • 96
    ISSN: 1432-0878
    Keywords: Neuropeptide ; Intracellular localization ; Sympathetic ganglia ; Colchicine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In colchicine-pretreated cells of sympathetic ganglia, intensely NPY-immunoreactive material was localized within vacuoles and vesicles of the disorganized, widely dispersed Golgi apparatus. Intensely positive large granular vesicles, which are known to be one of major storage sites of various peptides in the autonomic nerve endings, were essentially unobserved in the perikaryal cytoplasm. The present finding provides evidence that one pool of NPY-like immunoreactivity is localized in the Golgi apparatus of colchicine-pretreated as well as normal sympathetic ganglion cells. It is also clear that visualization of NPY-immunoreactive somata by colchicine-pretreatment in the sympathetic ganglia is due to the accumulation of the neuropeptide in the disorganized Golgi stacks instead of increased amount of the large granular vesicles containing NPY.
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  • 97
    ISSN: 1432-0878
    Keywords: Receptor-mediated endocytosis ; Chondroitin sulphate ; Colloidal gold ; Liver endothelial cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Endocytosis via the hyaluronic acid/chondroitin sulphate receptor of rat liver endothelial cells was studied ultrastructurally, by use of a probe consisting of chondroitin sulphate proteoglycan attached to 15-nm gold particles. The probe bound to the surface of the cells exclusively in coated regions of the plasma membrane. Internalization at 37° C took place in less than one minute during which time interval the bound probe was transferred to coated vesicles. Further transfer to lysosomes was delayed in association with an accumulation of probes in a prelysosomal compartment consisting of large vacuoles in which probes lined the inner aspect of the membrane. Transport to lysosomes occurred only after a lag phase of at least 40–60 min at 37° C.
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  • 98
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    Protoplasma 145 (1988), S. 195-199 
    ISSN: 1615-6102
    Keywords: Microtubule-associated proteins ; Development ; Rat ; Quail ; Xenopus laevis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the adult rat brain, MAP 2 is a high-molecular weight protein that is highly concentrated in dendrites. Immunoblots of homogenates of developing rat brain have indicated that a low-molecular weight form of MAP 2, MAP 2 c, is transiently expressed as the brain is undergoing morphogenesis. Using MAP 2-specific monoclonal antibodies, we have demonstrated that the compartmentalization of high-molecular weight MAP 2 and the developmental regulation of MAP 2 are conserved in mammalian, avian, and amphibian brain. We have also determined the distribution of MAP 2 c in developing neuronal tissue. MAP 2 c appears before high-molecular weight MAP 2 in developing neurons, and in contrast to the dendrite-specific high-molecular weight forms of MAP 2, MAP 2 c is present in axons and glia. We have also shown that MAP 2 c is present in the adult rat retina, where it is concentrated in regenerative photosensitive cells. The transient expression of MAP 2 c in the developing brain of three species as well as in adult photosensitive cells suggests a role for this protein in neurite growth and plasticity.
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  • 99
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    Plant systematics and evolution 160 (1988), S. 77-89 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Triticum aestivum ; T. timopheevi ; T. monococcum ; Dasypyrum villosum ; B, G, S, A, V genomes ; Nor-loci ; DNA-DNA hybridization ; spacer rDNA sequences ; molecular evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheNor-loci of polyploid wheats and their putative diploid progenitor species were assayed by probing isolated nuclear DNA with ribosomal DNA spacer sequences (spacer rDNA sequences, isolated by cloning), from theNor-loci of genomes B (Triticum aestivum), G (T. timopheevi), B (syn. S,T. speltoides), A (T. monococcum) and V (Dasypyrum villosum). DNA samples for analysis were digested with the restriction endonuclease Taq 1 and assayed by DNA-DNA hybridization under standard (37°C) and high stringency (64°C) conditions. The assay procedure emphasized differences between the divergent spacer sequences of the polyploid species and allowed relative homologies to the respective sequences in diploid species to be established. — The studies indicated thatT. timopheevi andT. speltoides contain different sets of spacer rDNA sequences which were readily distinguishable and, in the case ofT. timopheevi, assigned toNor-loci on different chromosomes. This contrast with the spacer rDNA sequences of the majorNor-loci on chromosomes 1 B and 6 B inT. aestivum, which were difficult to distinguish and were deduced to contain very similar sequences. Among the diploid progenitor species only the spacer rDNA fromT. speltoides shared close homology with polyploid wheat species. OneNor-locus inT. timopheevi (on chromosome 6 G) did not show close homology with any of the rDNA spacer probes available. — The data suggestsT. speltoides was the origin of someNor-loci for both theT. timopheevi andT. turgidum lines of tetraploid wheats. The possibility that the 6GNor-locus inT. timopheevi may have derived from an unknown diploid species by introgressive hybridization is discussed. The spacer rDNA sequence probe fromT. monococcum shared good homology with some accessions ofD. villosum and a line ofT. dicoccoides; the implications of this finding for evolution of present-day wheats are discussed.
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  • 100
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    Euphytica 39 (1988), S. 137-144 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum vulgare ; barley ; boron toxicity ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and yield of seven wheat and two barley cultivars or lines, previously found to show different degrees of boron tolerance under field conditions, were compared in a pot experiment at a range of soil boron treatments. Soil treatments ranged up to 150 mg/kg applied B. Extractable B in soils ranged up to 103 mg/kg. At the highest B treatment seedling emergence was delayed, but the percentage emergence was not reduced. The degree of boron toxicity symptom expression varied between the wheat cultivars and lines, with the two most tolerant, Halberd and (Wq*KP)*WmH)/6/12, displaying the least symptoms. The concentration of boron applied to the soil which produced a significant depression of growth and yield varied between cultivars. For example, the yield of (Wq*KP)*WmH)/6/12 was not affected at the 100 mg/kg applied boron treatment, while the grain yield for (Wl*MMC)/W1/10 was significantly reduced at the 25 mg/kg treatment. There was a linear increase in boron concentration in tillers at the boot-stage with increasing concentration of boron in the soil. The most boron tolerant genotypes had the lowest tissue boron concentrations in each of the treatments. Halberd and (Wq*KP)*WmH)/6/12 had approximately half the boron concentrations of the more sensitive genotypes at the 25 and 50 mg/kg treatments. Differential tolerance of boron within the tissue was also observed. Both Stirling and (Wl*MMC)/W1/10 had significantly reduced total dry matter and grain yields at the 25 mg/kg treatment, while the concentrations of boron in boot stage tillers at this treatment were 118 and 100 mg/kg, respectively. On the other hand, Halberd and (Wq*KP)*WmH)/6/12 had tissue boron concentrations of 144 and 131 mg/kg, respectively, at the 50 mg/kg treatment but yield was unaffected. The relative responses in the pot experiment, for wheat, were in close agreement with field results. Halberd and (Wq*KP)*WmH)/6/12 had the highest grain yields, with the lowest concentrations of boron in the grain when grown under high boron conditions in the field. In pots these two genotypes proved to be the most tolerant of boron. For barley the advantage in grain yield in the field, expressed by WI-2584 compared with Stirling, was not repeated in pots. WI-2584 was, however, more tolerant than Stirling on the basis of total dry matter production. The results show that useful variation in boron tolerance exists among wheat, and that breeding should be able to provide cultivars tolerant to high levels of boron.
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