Publikationsdatum:
1990-06-29
Beschreibung:
The human immunodeficiency virus (HIV) tat protein (Tat) is a positive regulator of virus gene expression and replication. Biotinylated Tat was used as a probe to screen a lambda gt11 fusion protein library, and a complementary DNA encoding a protein that interacts with Tat was cloned. Expression of this protein, designated TBP-1 (for Tat binding protein-1), was observed in a variety of cell lines, with expression being highest in human cells. TBP-1 was localized predominantly in the nucleus, which is consistent with the nuclear localization of Tat. In cotransfection experiments, expression of TBP-1 was able to specifically suppress Tat-mediated transactivation. The strategy described may be useful for direct identification and cloning of genes encoding proteins that associate with other proteins to modulate their activity in a positive or negative fashion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nelbock, P -- Dillon, P J -- Perkins, A -- Rosen, C A -- New York, N.Y. -- Science. 1990 Jun 29;248(4963):1650-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Hoffmann-La Roche Inc., Nutley, NJ 07110.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2194290" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Amino Acid Sequence
;
Animals
;
Cell Line
;
Cloning, Molecular
;
DNA, Neoplasm/genetics
;
DNA-Binding Proteins/*genetics/metabolism
;
Escherichia coli/genetics
;
Gene Expression
;
Gene Library
;
Gene Products, tat/*metabolism
;
HIV/genetics
;
Humans
;
Molecular Sequence Data
;
Plasmids
;
Polymerase Chain Reaction
;
*Proteasome Endopeptidase Complex
;
Recombinant Fusion Proteins/metabolism
;
Trans-Activators/*metabolism
;
Transcriptional Activation
;
Transfection
;
tat Gene Products, Human Immunodeficiency Virus
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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