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  • Conservation  (2)
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  • Universidade Estadual de Maringá. Centro de Ciências Agrárias. Programa de Pós-Graduação em Zootecnia  (1)
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  • 1
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    Universidade Estadual de Maringá. Centro de Ciências Agrárias. Programa de Pós-Graduação em Zootecnia
    Publikationsdatum: 2021-05-19
    Beschreibung: Cryopreservation of genetic material of fish has become a tool essential for preservation of biological materials and production. However, stocking of fish embryos in liquid nitrogen (-196°C) and maintain indefinitely is not yet possible. Recently, developments in research on cryopreservation allowed storing embryos at -8°C for 24 hours. This indicates the possibilityof fish embryo cryopreserving, moreover, the biotechnical cooling for a short time already has practical applications. The present study aimed to evaluate the efficiency of different cryoprotectants to the processes of cryopreservation (freezing, vitrification and cooling) of tambaqui embryos (Colossoma macropomum). Despite not having succeeded in tambaqui embryos cryopreservation important points has been clarified as related to damage to the embryos. Injuries and morphological changes in embryos were identified at all stages of the freezing process, being higher with stabilization at -35°C after being submerged in liquid nitrogen and thawed. At the moment of the seeding besides a lower incidence of injuries, higher percentage of embryos with chorion were observed. The freezing curve protocolstested have not prevented the formation of ice crystals, thus turning unfeasible the embryonic embryos C. macropomum freezing. In the experiment of vitrification the methanol 20% with two-minutes of pre-immersion in exposure to liquid nitrogen, preserved the chorion and some cellular structures. Although it avoided further damage was not sufficient to prevent mortality of embryos, these results open perspectives for continued studies on cryopreservation of fish embryos. Ethylene glycol, glycerol and DMSO were toxic at higher concentrations (20 and30%) not being interesting for the vitrification of embryos C. macropomum. In tests of cooling to at -8°C treatments with ethylene glycol, glycerol and DMSO associated withsucrose did not result in satisfactory answers. To store embryos of C. macropomum at -8°Cfor a period of six hours is suggested cryoprotectant solutions with 17% sucrose combinedwith 10% methanol.
    Beschreibung: A criopreservação do material genético de peixes tornou uma ferramenta imprescindível,tanto para conservação de biodiversidade como para produção. No entanto, estocar embriões de peixes em nitrogênio líquido (-196ºC) e conservar por tempo indeterminado ainda não é possível. Recentemente, a evolução nas pesquisas com criopreservação possibilitou estocar embriões a -8ºC, por até 24 horas. Este fato aponta a possibilidade de criopreservar embriões de peixe, e a biotecnia de resfriamento por curto período já tem aplicações práticas. O presente estudo teve como objetivo avaliar a eficiência de diferentes crioprotetores nos processos da criopreservação (congelação, vitrificação e resfriamento) de embriões de tambaqui (Colossoma macropomum). Embora parte dos objetivos propostos tenha sido alcançados, nos testes de criopreservação, somando ao esclarecimento dos aspectos importantes quanto aos danos causados aos embriões, existem ainda muitas etapas para tornar viável a criopreservação de embriões de peixes. Nos testes, foram identificadas injúrias nos embriões em todas as etapas do processo de congelação, sendo a fase com maior incidência na estabilização em -35ºC e após a submissão em nitrogênio líquido e posterior descongelamento. No momento do ?seeding? (indução a cristalização a -7ºC), além de menor ocorrência de injúrias, maior porcentagem de embriões com córion foram verificados. As curvas de resfriamento nos testes de congelação não evitaram a formação de cristais de gelo, o que inviabilizou a congelação dos embriões de C. macropomum. No experimento de vitrificação, o metanol 20% com dois minutos de exposição pré-imersão ao nitrogênio líquido preservou o córion e algumas estruturas celulares. Embora tenha evitado maiores danos não foi suficiente para evitar a mortalidade dos embriões, o que abre perspectivas para continuidade nos estudos em criopreservação de embriões de peixes. Etilenoglicol, DMSO eglicerol foram tóxicos nas concentrações mais elevadas (20 e 30%), não sendo interessante para a vitrificação dos embriões de C. macropomum. Nos testes de resfriamento a -8ºC, os tratamentos com etilenoglicol, glicerol e DMSO associados à sacarose não resultaram em respostas satisfatórias. Para armazenar embriões de C. macropomum a -8°C, por um período de seis horas, sugere-se soluções crioprotetoras com sacarose 17% associada ao metanol 10%.
    Beschreibung: PhD
    Schlagwort(e): Cryoprotectants ; Conservation ; Animal production ; Genetics ; Tambaqui ; Brazil ; Produção animal ; Genética ; Tambaqui ; Reprodução ; Biotecnologia ; Crioprotetores ; Conservação ; Biotechnology ; Brasil ; Reproduction
    Repository-Name: AquaDocs
    Materialart: Thesis/Dissertation
    Format: 91pp.
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 2
    Publikationsdatum: 2022-10-20
    Beschreibung: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Baumgartner, M. F., Bonnell, J., Van Parijs, S. M., Corkeron, P. J., Hotchkin, C., Ball, K., Pelletier, L., Partan, J., Peters, D., Kemp, J., Pietro, J., Newhall, K., Stokes, A., Cole, T. V. N., Quintana, E., & Kraus, S. D. Persistent near real-time passive acoustic monitoring for baleen whales from a moored buoy: System description and evaluation. Methods in Ecology and Evolution, 10(9), (2019): 1476-1489, doi: 10.1111/2041-210X.13244.
    Beschreibung: 1. Managing interactions between human activities and marine mammals often relies on an understanding of the real‐time distribution or occurrence of animals. Visual surveys typically cannot provide persistent monitoring because of expense and weather limitations, and while passive acoustic recorders can monitor continuously, the data they collect are often not accessible until the recorder is recovered. 2. We have developed a moored passive acoustic monitoring system that provides near real‐time occurrence estimates for humpback, sei, fin and North Atlantic right whales from a single site for a year, and makes those occurrence estimates available via a publicly accessible website, email and text messages, a smartphone/tablet app and the U.S. Coast Guard's maritime domain awareness software. We evaluated this system using a buoy deployed off the coast of Massachusetts during 2015–2016 and redeployed again during 2016–2017. Near real‐time estimates of whale occurrence were compared to simultaneously collected archived audio as well as whale sightings collected near the buoy by aerial surveys. 3. False detection rates for right, humpback and sei whales were 0% and nearly 0% for fin whales, whereas missed detection rates at daily time scales were modest (12%–42%). Missed detections were significantly associated with low calling rates for all species. We observed strong associations between right whale visual sightings and near real‐time acoustic detections over a monitoring range 30–40 km and temporal scales of 24–48 hr, suggesting that silent animals were not especially problematic for estimating occurrence of right whales in the study area. There was no association between acoustic detections and visual sightings of humpback whales. 4. The moored buoy has been used to reduce the risk of ship strikes for right whales in a U.S. Coast Guard gunnery range, and can be applied to other mitigation applications.
    Beschreibung: We thank Annamaria Izzi, Danielle Cholewiak and Genevieve Davis of the NOAA NEFSC for assistance in developing the analyst protocol. We are grateful to the NOAA NEFSC aerial survey observers (Leah Crowe, Pete Duley, Jen Gatzke, Allison Henry, Christin Khan and Karen Vale) and the NEAq aerial survey observers (Angela Bostwick, Marianna Hagbloom and Paul Nagelkirk). Danielle Cholewiak and three anonymous reviewers provided constructive criticism on earlier drafts of the manuscript. Funding for this project was provided by the NOAA NEFSC, NOAA Advanced Sampling Technology Work Group, Environmental Security Technology Certification Program of the U.S. Department of Defense, the U.S. Navy's Living Marine Resources Program, Massachusetts Clean Energy Center and the Bureau of Ocean Energy Management. Funding from NOAA was facilitated by the Cooperative Institute for the North Atlantic Region (CINAR) under Cooperative Agreement NA14OAR4320158.
    Schlagwort(e): Acoustics ; Autonomous ; Buoy ; Conservation ; Mitigation ; Real‐time ; Ship strikes ; Whale
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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