Publication Date:
2015-11-28
Description:
Vibrational spectroscopy has been extensively applied to the study of molecules in gas phase, in condensed phase, and at interfaces. The transition from spectroscopy to spectroscopic imaging of living systems, which allows the spectrum of biomolecules to act as natural contrast, is opening new opportunities to reveal cellular machinery and to enable molecule-based diagnosis. Such a transition, however, involves more than a simple combination of spectrometry and microscopy. We review recent efforts that have pushed the boundary of the vibrational spectroscopic imaging field in terms of spectral acquisition speed, detection sensitivity, spatial resolution, and imaging depth. We further highlight recent applications in functional analysis of single cells and in label-free detection of diseases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cheng, Ji-Xin -- Xie, X Sunney -- New York, N.Y. -- Science. 2015 Nov 27;350(6264):aaa8870. doi: 10.1126/science.aaa8870.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Weldon School of Biomedical Engineering and Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. jcheng@purdue.edu xie@chemistry.harvard.edu. ; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. jcheng@purdue.edu xie@chemistry.harvard.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26612955" target="_blank"〉PubMed〈/a〉
Keywords:
3T3-L1 Cells
;
Animals
;
Biology
;
Gases
;
Humans
;
Medicine
;
Mice
;
Microscopy/*methods
;
Molecular Imaging/*methods
;
Sensitivity and Specificity
;
Spectrum Analysis, Raman/*methods
;
Vibration
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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