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  • 101
    Publication Date: 2021-05-19
    Description: Bahmanshir River derived from Karoon River and finally connected to Arvandrood that passed from Abadan and then flow into the Persian Gulf. Because of low slope of Bahmanshir River from Ahwaz to Abadan, tidal flow intrusion long distance in Bahmanshir, Arvandrood and Karoon. Sampling from physical and chemical parameters and phytoplankton were done by Nansen sampler, benthic sampling, heavy metals, TOM and grain size sampling were done by grab. Samplings of zooplankton were done by filtering 20 liters water in 100μ mesh size net from middepth of river water. Fish sampling were done by gill net, Trawl net, sobor gill net and stable net with 20, 27, 30, 35, 39, 40 mesh sizes. Physical and Chemical parameters were more similar from station one to four but there were some differences with station five in mouth of river in the sea. Nitrate showed same variations in all studied stations and maximum concentration of nitrite was observed in station five in June and July. The highest value of Total hardness and salinity were observed in station five. The range of pH was 7.2 to 8.5. Cd and Zn were found to be the lowest and the highest concentration in sediments respectively. Annual average following as: Cd 〈 Co 〈 Cu 〈 Pb = Ni 〈 Zn According to ISQGs and river water quality standards, chemical and physical parameters and sediment heavy metals were in acceptable range. Only Cd and Zn values were found higher than acceptable ranges. No significant difference was observed between stations for heavy metal concentrations. In general, 44 phytoplankton species were identified Bacillariophycea (77.74%), Cyanophycea( 10.39%), (Chlorophycae 8.88%) and (Dinophycea 2.99%) were the dominant phytoplankton classes in this study. Shannon index have shown the highest species diversity during one year in January and the lowest was in fourth station. 110 Phytoplankton species composition in Bahmanshir have shown that Bacillariophycea became higher from river to estuary and among zooplanktons Rotifera and Tintinida were the most frequent groups in estuary region with 83% of total abundance. Copepoda, Rotifera and Protozoa (especially Tintinida) were the most frequent zooplankton groups. The highest frequency of zooplankton with 40.6% was observed in the station one in Khoramshahr. 16 macrobenthic groups were identified during the study. Polycheate worms, Isopoda and Oligocheate worms have shown the 83.5% , 5.4% and 3% frequencies respectively. 43 fish species that included in 26 families were identified. Cyprinidae with 12 species and Clupeidae with 2 species were the highest and the lowest species frequencies respectively. Among the identified fish species Hypophthalmichthys molitrix, Barbus esocinus, Crrassius auratus were exotic species and rest of them were local species. Seasonal changes were affected directly by quantitative and qualitative variations in Karoon water and planktonic species composition in estuary region were affected by tidal flow and advances of seawater. Cyprinidae and Barbus geniuses were the most frequent fresh water fish species. Migrant species like Johnius dussumeieri, Acanthophagus latus, Hilsa ilisha were captured in most stations and months, presence of these species is important for reproduction. Fish species of this river have different diet that included soft sediments, planktons, benthos, macroscopic plants, small fishes and shrimps.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physical ; Chemical ; Limnological ; Sampling ; Phytoplankton ; Zooplankton ; Salinity ; pH ; Bacillariophyceae ; Cyanophycea ; Chlorophycae ; Dinophycea ; Polycheate ; Oligocheate ; Hypophthalmichthys molitrix ; Barbus esocinus ; Heavy metal
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 110pp.
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  • 102
    Publication Date: 2021-05-19
    Description: Shrimp diseases, especially viral diseases are the major limiting factors of shrimp farming activities in the world. White spot disease is one of the most pernicious viral disease that lead to serious loss in all shrimps of penaeids family. In Iran during 2002 to 2004 in Khuzestan province and in 2005 in Bushehr province, the most ponds and farms infected with white spot and the entire industry faced threat of closure. Surface proteins of virus has important role in the early stages of virus with host cell interaction, because these proteins usually enumerated as a candidate to produce a subunit and recombinant vaccines. On the other hand, these proteins can use as an important virus antigen and produce virus-specific antibodies in the preparation of diagnostic kits. In several studies proteins in the form of injection or oral vaccine has shown promising results. This study was designed in order to gain these proteins for immunization of white leg shrimp. To achieve the white spot virus genome, sampling and DNA extraction of moribund shrimps with clinical signs was done from Choebdeh farms. VP28 and VP19 protein-coding gene-specific primers were selected, and after amplification and purification were cloned in E.coli TG1. Protein expression evaluated and commercial plates were coated with inactivated bacteria containing the recombinant protein. White leg shrimp post larvae (PL30) were fed with recombinant food for 7 days then two times in 9th and 23th day after beginning of plate feeding and was challenged by white spot disease virus. The results of the first exposure (end of the seventh day after the shrimp fed with recombinant plate) showed that the lowest mortality at day 14 after exposure in different groups belong to VP28 group with cumulative mortality percent %30 ± 3.84 and the highest percentage of the Group TG1 equal to %72.22±2.93, also The highest and lowest relative percent survival, belong to the group of VP28 (60.29± 5.09%) and TG1 (4.41 ± 2.94%), respectively. In terms of mortality percent and relative percent survival, VP28 and VP 28+19 has significant difference with other groups while there was no significant difference between the groups VP19, TG1 and pMal. In the second exposure experiment (21 days after end of plate feeding) results showed that the lowest cumulative mortality in different groups belong to VP28 (%50 ± 5.09) and the highest percentage was owned by Group TG1 equal to %75.55±2.22, also the highest and the lowest of relative percent survival belong to VP28 (%32.86± 6.83) and TG1 (%0.00 ± 2.98) groups respectively. Similar to the first experiment, VP28 showed significant differences in cumulative mortality and relative percentage of survival in contrast to other group. As a general conclusion it can be noted that recombinant protein VP28 in any form that delivered to shrimp was able to protect shrimp against white spot virus. On the contrary VP19 has no this ability. The survival rate is directly related to the duration of the presence of recombinant protein in the diet.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Shrimp ; WSSV ; Vaccine ; In vitro ; White spot disease ; Proteins ; DNA ; Larvae ; Mortality ; Survival ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 103
    Publication Date: 2021-12-05
    Description: Regarding the fish farming in cages, taking into account all nutritional requirements appropriate to the needs of aquaculture is essential in order to minimize damage to the environment and achieve maximum growth. Fish nutritionists should be aware about the analysis of ability to digest and absorb nutrients in the diet to prepare and adjust the diet according to the needs of aquaculture. Food must also have appropriate physical properties for food intake with minimal loss of nutrients in the water. Inadequate resources with low quality or energy consumption within food, reduced the growth performance and increased the excretion of nitrogen in the environment. Food must be designed so that the balance of minerals in food will help maintain osmotic pressure within the body of fish in salty water. Food requirements and efficiency of food conversion ratio (FCR) is variable by changing environmental conditions (dissolved oxygen, temperature, water quality, flow rate, light intensity ,day length). The fish of cages are feeding by floating pellets which have the ability to stand in the water column. The floating pellets allows the fish to have sufficient time and opportunity for finding food. But sinking pellets pass rapidly from the cage and are inaccessible to fish. According to the Caspian sea conditions is better the extruded food equipped with Hygenizer to be used that a portion of it remains on the water surface and part of it moves gently down with an immersion mode in the water column (Softly deposited) for fish consumption. The amount, number and time of feeding are important factors to assess the nutritional activities. In general, fish feed from dry food between 2 to 3 percent of their body weight. Food consumption was also varied due to the quality of the food and fish physiological factors such as age, size, stage of life and stress level. Fish food distribution being fed manually or by means of demand, launcher and automatic feeding. The farm managers should pay attention to the things like water flows in the cage, wind, fish appetite, consolidated flows in the cage during food distribution as well as food storage in dry places with proper ventilation system to prevent the growth of fungi and insects activities to prevent loss of food. Also, adding antioxidants to the 100150 ppm when storing dry foods including the important cases particularly in prevention of fish liver lipoidosis disease is considered.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cage culture ; Salmon ; Feeding ; Analyze ; Nutrition ; Variety ; Fish ; FCR ; Aquaculture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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