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  • Neurosecretion  (52)
  • Synapses  (37)
  • Springer  (89)
  • Essen : Verl. Glückauf
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • 2015-2019
  • 2005-2009
  • 1970-1974  (89)
  • 1940-1944
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 149 (1974), S. 205-221 
    ISSN: 1432-0878
    Keywords: Locus coeruleus ; Tegmentum ; Mesencephalon ; Adrenergic centers ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The locus coeruleus of cat is populated by two types of neurons: medium sized ones, with plump cell bodies and relatively short dendrites; and small ones, with triangular bodies and relatively long dendrites. The former type is regarded here as typical of the centre, whereas the second type could simply represent displaced neurons from the adjacent griseum centrale. Electron microscopy failed to reveal any outstanding richness in pigment granules in kittens up to five weeks old. Very characteristic somatic appendages were found, mostly in the medium sized neurons. These somatic “spines” communicate with the perikaryon by means of a narrow neck region. A complex, multilayered, glial sheath surrounds the cells. This glial sheath is pierced by the somatic appendages, which are not surrounded by glia and make contact with axonal knobs. Typical dendritic spines appear to be absent. Axodendritic synapses are made on medium sized dendritic trunks. By and large, most of the synaptic vesicles present in the centre are of the small, clear-centered type. However, dense core vesicles extremely variegated in size and appearance were found, both in presynaptic and postsynaptic profiles. The possibility that dense core vesicles should be regarded as atypical lysosomes rich in by-products of the metabolism of catecholamines (melanine) has been considered.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 152 (1974), S. 283-292 
    ISSN: 1432-0878
    Keywords: Sense organ ; Lateral line ; Synapses ; Ambystoma mexicanum ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lateral line organs in young salamanders of the species Ambystoma mexicanum were investigated with scanning and transmission electron microscopy. They were found to differ from the lateral line organs in adult animals (1) by being lower, having short hair cells and supporting cells, (2) by the hair cells having areas of lateral contact, (3) by the occasional presence, at the edge of the organ, of hair cells at an early developmental stage. Two types of nerve endings are seen: (1) afferent, and (2) less commonly, efferent vesiculated ones. Synaptic bodies have been seen in the cytoplasm without association to afferent synapses.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 259-275 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Neurosecretion ; Acipenseridae ; NaCl load
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurohypophyses of sexually mature Acipenser güldenstädti Brandt and Acipenser stellatus Pallas maintained in hypertonic solutions were studied by means of light and electron microscopy. The fish were caught in the Ural and transferred into basins containing either freshwater (controls) or 32‰, 22‰ and 17‰ NaCl solutions respectively. The neurohypophyses of control animals kept in freshwater for two days contained a large amount of “Gomori-positive” neurosecretory material (4 arbitrary units). An abundance of neurosecretory granules of different kinds, predominantly elementary ones, was observed electron microscopically in neurosecretory fibres and terminals of these fish. A decrease in the amount of neurosecretory material was found in all experimental animals: up to 1–2 units in A. güldenstädti after 3.5–10 h in 32‰ NaCl, and up to 3.5 units in A. stellatus after 4.5–48 h in 22‰ or 17‰ NaCl. The number of neurosecretory granules decreased considerably in the fibres and terminals in all experimental series, but that of granulated, disintegrating granules and “granule-shadows” increased markedly, especially in preterminal parts of axons. An increased number of hypertrophied mitochondria, lamellar bodies, residual granules and especially “synaptic” vesicles was seen. These changes speak for an active discharge of neurohormones into the general circulation, a phenomenon well known to be characteristic of stress responses. Pathological ultrastructural changes (breakdown of terminals, extracellular wide cisternae) were visible in A. güldenstädti neurohypophyses at the end of the experiments. Also tanycytes — “narrow” ependymal cells — and pituicytes underwent noticeable changes.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 150 (1974), S. 213-229 
    ISSN: 1432-0878
    Keywords: Nerve cells ; Regeneration ; Neurosecretion ; Hydra viridis, littoralis ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using whole mount preparations, nerve cells at the cut surface (head region) and also the peduncular-basal disk region were studied during various stages of regeneration (zero hour — 96 h) in Hydra. Leucomethylene blue staining technique allows excellent stainability of nerve cells and thus a statistical count of them at the specified stages of regeneration was obtained. Within 1 h after transection a significant increase (P 〈 0.001) in release of neurosecretory droplets occurs. Between 4 and 15 h of regeneration the number of perikarya remains relatively constant, but the number of processes increases by 200%. This indicates that neurons in Hydra have the capacity to regenerate their processes. Their growth in length is dramatically illustrated at 18 hrs. of regeneration. This degree of anastomosing arrangement is not observed in any other stage of regeneration. After this time the majority of processes break down releasing the neurosecretory materials. A study of the number of perikarya, the number of neurites and the number of neurosecretory droplets in regenerating animals reveals a continuous increase in the number of nerve cells and neurosecretory droplets released for up to 24 h. With the accumulation of nerve cells at the cut surface (24 h of regeneration) there is a simultaneous appearance of tentacle outpushings. The tentacles increase in number and length during the subsequent periods of regeneration. As the regenerative process approaches completion (72–96 h) the number of neurosecretory droplets released decreases, approaching the pre-transection levels as seen in normal animals. It is suggested that the neurosecretory material may act as a “trophic” agent which stimulates differentiation of interstitial cells into nerve cells and thus influences the regenerative process.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 150 (1974), S. 231-247 
    ISSN: 1432-0878
    Keywords: Regeneration ; Nerve cells ; Neurosecretion ; Hydra ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Major ultrastructural changes in neurons were studied during sequential periods of hypostomal regeneration in Hydra. Some neurons remain unaffected except that at certain periods following amputation, they become more active in neurosecretory production. Other neurons in various stages of differentiation were also observed. Most emphasis was placed on degenerating neurons showing a loss of organelles and destruction of the perikarya. Certain large, membrane-bounded structures (up to 1.7 μ in diameter) suggested tentatively to be of a lysosomal-like nature, may be partly responsible for the degenerative process. The neurites of these cells first assume a beaded appearance and/or contain bulbous endings. The eventual isolated fragments of neurites contain typical membrane-bounded neurosecretory droplets (850/1700 Å in diameter) which disintegrate forming particulate materials (350 Å in diameter). Following complete disruption of the neurites, some of the granules accumulate in the extracellular spaces before they are disintegrated. From the data presented in this and the preceding paper, it is suggested that these particles, derived from neurosecretory droplets, may be responsible for the stimulation of: 1) interstitial cell differentiation into neurons, 2) the increased activity in neurosecretory production in normal cells, 3) the conspicuous increase in neurite length and consequently the exaggerated degree of anastomosis, and 4) the possible capacity of neurons to regenerate neurites.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 153 (1974), S. 101-113 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Neurohypophysis ; Neurosecretion ; Axoplasmic transport ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The lower pituitary stalk has been studied in rats given hypertonic saline for four days and in rats with congenital diabetes insipidus (GDI), that is, in two groups of animals in which there is evidence of an increase in the transport of polypeptides down the axons of the tractus hypophyseus. A quantitative ultrastructural analysis of this tract, the first yet made, has demonstrated that in normal animals it is composed of unusually small axons, over half of which are less than 300 nm in diameter. In this tract there is a significant increase in axonal diameter and microtubular content in animals given hypertonic saline for four days. In adult rats with CDI, these changes are even more marked, a feature which is correlated with the fact that in these animals the transport of polypeptides has probably been abnormal since birth. An alteration in the microtubular content of neurons has not previously been observed either in physiologically stressed animals or in diseased animals; such alterations suggest the participation of microtubules in polypeptide transport. The narrow diameter of axons in the tractus hypophyseus of the normal animal raises the possibility of the extravesicular transport of such polypeptides.
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  • 7
    ISSN: 1432-0878
    Keywords: Synapses ; Embryo (Xenopus laevis) ; Spinal cord ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rostro-caudal gradient of differentiation found in vertebrate embryos has been utilized to examine the sequence of synaptic junction development in the spinal cord of Xenopus laevis at a late embryonic stage. Uniform samples were taken at various points along the cord of a stage 27 embryo and examined in the electron microscope. The general ultrastructure of the cord demonstrated the rostro-caudal gradient of development. The sequence of synaptic junction development was like that in the cervical region (Hayes and Roberts, 1973). “Membrane-vesicle clusters” and “immature” synaptic junctions were found most caudally followed by synaptic junctions, first with cleft and subsynaptic membrane density, then with only cleft density and finally, most rostrally, with cleft, subsynaptic membrane, and subsynaptic cytoplasmic density. Mature synaptic junctions were found in increasing numbers from the mid to anterior trunk cord and could mediate alternating trunk flexions made by the embryos at this stage of development. “Membrane-vesicle clusters” were found near processes containing irregular vesicles and also near membrane outlines. These may be signs of dendritic growth. “Membrane-vesicle clusters” were also found in varicosities, facing the space around the spinal cord and in nerve fibres peripherally between the skin and myotomes. This suggests an association of early stages in synaptogenesis with axon growth. This and other possible inferences about axon and dendrite growth in relation to synaptogenesis are discussed.
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  • 8
    ISSN: 1432-0878
    Keywords: Infracerebral complex ; Polychaeta, Nereidae ; Neurosecretion ; Fine structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The infracerebral complex consists of: (a) two types of ependymoid infracerebral cells located on the ventral surface of the brain, adjacent to a coelomic sinus and blood vessels; (b) a noncellular stroma, the neural lamella, which separates the infracerebral cells from cerebral nervous tissue; (c) two types of cerebral neurosecretory endings (one with many mitochondria) which abut on the neural lamella opposite from the infracerebral cells. The infracerebral a cells become columnar and develop abundant apical caveolae and Golgi elements in older worms. The infracerebral b cells, filled with electron opaque granules, resemble neurosecretory cells; their axon-like processes contain microtubules and make synaptoid contacts with the neural lamella. Both cell types are present in young worms of about 10 segments but the b cells, relatively abundant in younger worms, are outnumbered by a cells in mature individuals. However, during reproductive development neither a nor b cells display any structural changes which would indicate that they represent glandular tissue. Basal processes originating both from a and b cells form entanglements which become progressively more intricate during maturation. Neurosecretory endings are present in 10-segment worms and become very numerous in mature worms but at no time do they penetrate the neural lamella and contact the infracerebral cells. It is unlikely that the infracerebral cells per se constitute a gland but they are strategically located to influence the release of hormones from neurosecretory endings.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 147 (1974), S. 199-207 
    ISSN: 1432-0878
    Keywords: Nucleus praeopticus ; Xenopus, tadpole ; Neurosecretion ; Osmoregulation ; Enzyme cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The activity of four enzymes was studied in the neurosecretory cells of the preoptic nucleus ofXenopus laevis (tadpoles and adults) in control and osmotically stimulated animals. Any activity of lactate dehydrogenase could not be demonstrated, whereas activity of thiamine pyrophosphatase, glucose-6-phosphate dehydrogenase and succinate dehydrogenase was demonstrated only in the cells of the caudo-dorsal region of the preoptic nucleus in experimental animals. It is concluded that the cells of this region may be involved in osmoregulatory processes.
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  • 10
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Harvestman ; Exocytosis ; Release ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les plaques paraganglionnaires de l'Opilion Trogulus nepaeformis sont des organes neurohémaux constitués d'une lamelle neurale externe, épaisse, en contact avec un sinus sanguin, d'une couche basale de cellules gliales, et de 7 types de terminaisons axonales (A1, A2, B1, B2, B3, B4 and C) renfermant des granules élémentaires qui diffèrent par leur taille, leur densité aux électrons et l'aspect de leur contenu. Les granules de type A1 se déchargent par exocytose dans l'espace extra-axonal, sous la lamelle neurale; ceux de type C se fragmentent en microgranules à contenu dense qui s'accumulent avec des vésicules claires contre l'axolemme en des places suggérant des sites de libération dans l'hémolymphe.
    Notes: Summary The paraganglionic plates of the harvestman, Trogulus, are neurohemal organs composed of a thick neural lamella facing a blood sinus, basal glial cells, and seven distinct types of axon terminals (A1, A2, B1, B2, B3, B4 and C) identified on the basis of elementary granule size, electron density, and structure of contents. The release of A1 type granules occurs by exocytosis into the extraaxonal space below the acellular neural lamella. The release of the content of C granules occurs after fragmentation into small electron dense vesicles. Clusters of these vesicles intermixed with clear vesicles pressing against the axolemma beneath the neural lamella, apparantly correspond to the sites of release.
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  • 11
    Electronic Resource
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    Springer
    Cell & tissue research 154 (1974), S. 275-288 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Insect ; Leptinotarsa decemlineata ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of seven types of neurosecretory cells (NSC) in the medial and lateral groups of the protocerebrum is described. The differences among cell types established earlier by light microscopy parallel differences in size and appearance of the neurosecretory particles observed in electron micrographs. No relationship was found between the affinity for Gomori's paraldehyde fuchsin stain and the nature of the particles. The secretions of the A-, A1-, and C-types of NSC of the medial group are characterized by electron-dense neurosecretory granules of 1250 Å dia., medium-dense granules of 2100 Å, and electron-lucent vesicles of 1700 Å, respectively. The L-type NSCof the lateral group contain smaller (1300 Å) or larger (1700 Å) neurosecretory granules. The medial B- and E-types of NSC and the lateral LB-type contain granulated vesicles (1200 Å) of the same appearance. These cell types differ in other respects and most likely have separate functions.
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  • 12
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    Springer
    Cell & tissue research 155 (1974), S. 117-125 
    ISSN: 1432-0878
    Keywords: Sinus gland ; Carcinus maenas (Crustacea, Decapoda) ; Nerve fibre cell types ; Neurosecretion ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sinus gland of Carcinus maenas consists of the swollen axonal endings of the neurosecretory cells of the major ganglia and acts as a storage release centre for the membrane bound neurosecretory material. These neurosecretory granules fall into five different types based on size and electron density. Their contents are released by exocytosis of the primary granules or smaller units budded from the primary granules.
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  • 13
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    Cell & tissue research 136 (1973), S. 461-477 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Acipenseridae ; Neurosecretion ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurohypophysis of sexually mature male and female Acipenser güldenstädti Brandt and Acipenser stellatus Pallas was studied light and electron microscopically. The recessus hypophysei lined with ependymal cells of two main types, “narrow” and “wide”, are in the center of the neurohypophysial “roots”. Processes from both cell types run radially to the basement membrane of the connective tissue layers abutting on the hypophysial intermediate lobe. Protrusions penetrating deep into the recessus hypophyseus are found in the apical parts of the “wide” cells. Pituicytes are rare in the neurohypophysis. The ultrastructure of both ependymal cell types and of the pituicytes is described. Nonmyelinated “Gomori-positive” (peptidergic) neurosecretory A1 and A2 type fibres and their terminals containing elementary neurosecretory granules (1400–1800 Å and 1000–1400 Å respectively) are the main structural elements of the neurohypophysis. Some “dark” and single myelinated neurosecretory fibres have been found. The adrenergic fibres (type B) were described earlier (Polenov et al., 1972a). The structural peculiarities of the neurohypophysis are discussed in functional and comparative-morphological terms.
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  • 14
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Annelida ; Sexuality and Development ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Während der sexuellen Entwicklung vonPlatynereis dumerilii steigt die Zahl der Neurone, die Merkmale einer sekretorischen Aktivität aufweisen, parallel zum Wachstum und zur Reifung der Keimzelle an. Die cytologische Differenzierung eines Neurons zur neurosekretorischen Zelle ist mit einer Steigerung der Synthese Cystin-haltigen Materials gegenüber konventionellen Neuronen gekoppelt. Neurosekretorische Zellen treten erstmals in Erscheinung, kurz bevor die Oocyten in die zweite Oogeniephase eintreten, während der die Vitellogenese abläuft. Aufgetragen gegen den Oocytendurchmesser ergibt die Zahl der Neurone, die sekretorisch aktiv werden und3H-Cystin inkorporieren, eine ansteigende Kurve. Die Kurve biegt auf ein Plateau, wenn die Oocyten eine kritische Größe erreichen. Diese Größe markiert jenes Entwicklungsstadium, von dem ab eine Enthirnung eine normale Reifung nicht mehr verhindert. Die Zahl synthetisch tätiger Neurone steight erneut an, wenn die Oocyten die kortikale Struktur des reifen Eies entwickeln. Das Maximum wird kurz vor der Metamorphose erreicht. Während der epitoken Umwandlung sinkt die synthetische Aktivität rasch ab, um im schwärmbereiten Tier gänzlich zum Stillstand zu kommen. In Tieren, die nach der kritischen Phase zur caudalen Regeneration veranlaßt werden, fällt die Zahl markierter neurosekretorischer Zellen auf ein Niveau ab, das für junge, „vorkritische“ Individuen charakteristisch ist. Entsprechend wird in jungen, vorkritischen Tieren, die erst wenige endokrine Neurone besitzen, die Differenzierung weiterer neurosekretorischer Zellen durch eine Regeneration nicht stimuliert. Der zeitliche Verlauf der synthetischen Aktivität im Zuge der Entwicklung und die durch eine Regeneration erzwungenen Veränderungen sind somit reziprok zur Aktivität des Gehirns in Bezug auf die Ausschüttung des Juvenilhormons. Die Hypothese wird zur Diskussion gestellt, die sinkende Produktion des Juvenilhormons werde von der steigenden Produktion eines antagonistischen neuroendokrinen Prinzips begleitet oder verursacht.
    Notes: Summary During sexual development ofPlatynereis dumerilii, the number of neurones exhibiting secretory activity rises in parallel with the growth and maturation of the germ cells. The cytological differentiation of neuroglandular properties is accompanied with an increased uptake of tritiated cystine. The first neurones developing the characteristics of secretory cells and incorporating cystine appear just before the oocytes enter the second growth phase which corresponds with vitellogenesis. When plotted against the oocyte diameter the number of labelled neurosecretory cells yields an ascending line, which becomes a plateau once the oocytes approach a critical size. This critical size defines the developmental stage beyond which decerebration does not prevent normal maturation. The neuronal synthetic activity rises again when the oocytes differentiate the cortical structure of ripe eggs. The curve displays its maximum slope at the onset of metamorphosis. During the epitokous transformation the synthetic activity decreases rapidly and is almost totally depressed when swarming is imminent. In animals induced to regenerate their posterior segments after they have passed the critical stage, the number of labelled neurosecretory cells becomes reduced to the precritical level. According to this, regeneration in young precritical animals containing only few endocrine neurones does not stimulate the development of further secretory neurones. Thus, the time course during maturation and the changes caused by regeneration are reciprocal to the activity of the brain with respect to the release of juvenile hormone. The question is thus raised as to whether the declining titer of juvenile hormone may be accompanied or caused by an increased production of an antagonistic principle related to maturation.
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  • 15
    ISSN: 1432-0878
    Keywords: Sympathetic ganglion ; Granule-containing cells ; Synapses ; Three-dimensional analysis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The surface of 4 granule-containing cells, in a cluster within the rat superior cervical ganglion, was studied by a serial sampling technique for electron microscopy. The result shows that all the 4 cells receive one, or three afferent synaptic boutons from the preganglionic fibers impinging upon their somata, and a somatic efferent synapse exists at two locations on each soma of the 2 of these cells. The postsynaptic element of the efferent synapse is observed to be represented by non-vesiculated and vesiculated segments of dendrites, soma and a possible axon collateral of the adrenergic principal neuron of the ganglion. There is a remarkably constant development of the attachment plaque between the granule-containing cells themselves, representing 1.7–2.3% of surface area for each cell. The surface area exposed to the extracellular space (covered only by a basal lamina) varies from 0.1 to 2.3% of the total perikaryal surface of the 4 cells. A tendency is noted that those cells without efferent synapses possess a more extensive area exposed to extracellular space than those forming somatic efferent synapse to the postganglionic elements.
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  • 16
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    Cell & tissue research 143 (1973), S. 117-133 
    ISSN: 1432-0878
    Keywords: Nervous system ; Ctenophores ; Intra-mesogleal nervous system ; Synapses ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural evidence is given of the occurrence of nervous elements in the mesoglea of Ctenophores based on the presence of the typical synapses of this phylum. In Beroids, nervous fibers from the ectodermal nerve-net cross the epithelial basal membrane and run through the mesoglea; they are devoid of any ensheathing cell. These neurites build highly differentiated synapses upon the muscles and upon peculiar cells, tentatively named mesenchymal cells. In Cydippids, nerve fibers and nerve cell-bodies have been observed in the mesoglea of the tentacles. The mesogleal core of each tentacle contains mesenchymal cells and a thick strand of neurons and neurites, forming a kind of elongated ganglion. Neurites of either the axial neurones or the epithelial nerve-net neurones form numerous radial nerve strands across the tentacular muscles. Interneural, neuro-muscular and neuro-mesenchymal junctions are very frequent in the tentacle. As far as the organization of the mesoglea is concerned, the Ctenophora thus appear closer to Turbellaria than to Cnidaria.
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  • 17
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    Cell & tissue research 143 (1973), S. 505-515 
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Paracervical ganglion (Rat) ; Neurosecretion ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two new types of structure of the cytoplasm of an autonomic neuron were described: (1) Inclusions of the rough ergastoplasmic reticulum, which were named “ergastosomes”, contain finely granular material with moderately electron opaque staining with both glutaraldehyde-OsO4 and KMnO4 fixations. A short comment was made on the nature of the contents of the ergastosomes. (2) Tubular formations of endoplasmic reticulum were found in the axons and/or dendrites of the paracervical ganglion.
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  • 18
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    Cell & tissue research 139 (1973), S. 369-396 
    ISSN: 1432-0878
    Keywords: Synapses ; Giant fibres ; Ventral nerve cord ; Lumbricus terrestris L. ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die proximalen Kollateralen der dorsalen Riesenfasern des Regenwurms wurden in Serienschnitten vom Soma bis zum Eintritt in die Riesenfaser verfolgt und im Hinblick auf ihre Feinstruktur und ihre synaptischen Kontakte Untersucht. Es finden sich sowohl chemische als auch elektrische Synapsen. Ihre Feinstruktur wird mit der bekannter Synapsen anderer Wirbellosen und Wirbeltiere verglichen. In beiden Riesenfasersystemen kommen efferente chemische Synapsen mit feinen postsynaptischen Verzweigungen vor, die anscheinend von Bauchmark-Motoneuronen stammen. Das Axon der medianen Riesenfaser weist darüber hinaus nur noch eine elektrische Synapse mit den Rieseninterneuronen auf. Demgegenüber erhalten die Kollateralen der lateralen Riesenfasern zahlreiche Afferenzen, die zum Teil als sensorische Fasern der Epidermis, multisegmentale Fasern der Hauptfaserzüge und Rieseninterneurone identifiziert werden konnten. Weitere Afferenzen stammen vermutlich von unisegmentalen Interneuronen her. Beide lateralen Riesenzellaxone bilden außerdem miteinander eine elektrische Chiasma-Synapse mit besonderen Membraneinfaltungen.
    Notes: Summary The proximal collaterals of the dorsal giant fibres of the earthworm were traced through serial sections from the cell bodies to the giant axons. Their structure and synaptic connections were examined. There are chemical as well as electrical synapses. Their fine structure is compared to that of other known invertebrate and vertebrate synapses. Both giant fibre systems have efferent chemical connections with thin postsynaptic arborizations which probably belong to ventral cord motoneurons. Moreover the median giant axon is connected by an electrical synapse with the giant interneurons. The lateral giant collaterals on the contrary receive many afferences through chemical synapses which were partly identified as sensory fibers from the epidermis, multisegmental axons from the main fibre bundles or giant interneurones. Other afferences probably come from unisegmental interneurones. In addition both lateral giant axons form an electrical chiasma synapse with special membrane folds.
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  • 19
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    Cell & tissue research 141 (1973), S. 301-317 
    ISSN: 1432-0878
    Keywords: Cnidaria ; Nervous system ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nervous system of juvenile and adult Ceriantheopsis americanus has been examined with the electron microscope. The nervous system is exclusively ectodermal, forming a plexus of fibres lying between the epithelium and the muscle layer. The plexus consists of three types of nerve fibre that vary in diameter from 0.1 to 20 μ, and a fourth type of fibre of uncertain nature. The reticulum, previously thought to be the ectodermal nervous system, is redescribed as the peduncles of epithelial supporting cells. Intraneural and neuromuscular synapses are described. About 97% of the interneural are polarized. The unpolarized synapses are a variety not previously described, consisting of a series of polarized zones, for which the name multipolarized synapse is proposed. Structurally, the interneural synapses are more complex than those previously seen in the Cnidaria. Presynaptic projections, periodic cleft densities, and a postsynaptic web are described. By contrast, the neuromuscular synapses bear no membrane specializations. On the basis of observed synaptic interactions of its components a preliminary model of the functional organization of the nervous system is proposed.
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    Cell & tissue research 145 (1973), S. 1-22 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Cerebral neurohemal organ ; Vesicle ; Acheta domesticus ; Nervus corporis cardiaci I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Six principal types of neurons are recognized in the median neurosecretory-cell group of the pars intercerebralis in the house cricket, Acheta domesticus. Four of the six cell types are neurosecretory neurons. The majority of the 900 cells contain granules 1500–3000 Å in diameter and are designated as type I. These neurons, which are associated with the nervus corporis cardiaci I (NCCI), undergo asynchronous secretory cycles. Two cell types (II and IV), which contain 700–1600 Å granules, terminate in a neurohemal area on the ventral median surface of the brain, a site not previously recognized as neurohemal in insects. Neurosecretory axons that lie within and below the perineurial sheath in the cerebral neurohemal area contain typical synaptoid figures, but no evidence of exocytosis was found. A single pair of neurosecretory cells (type III) has been recognized only from light microscope sections. Two distinct types of vesicle-bearing axons occur in protocerebral neuropile. The majority contain electron-dense and dense-cored vesicles 600–1000 Å in diameter. Some axons contain granules of type I cells and may be collaterals or loops of NCCI axons.
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  • 21
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    Cell & tissue research 144 (1973), S. 153-166 
    ISSN: 1432-0878
    Keywords: Arthropods Limulus ; Eye ; Neurosecretion ; Neuroeffector junction ; Nerve degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the protocerebrum of Limulus polyphemus, the horseshoe crab, neurosecretory cells are grouped within the curvature of the central body, an association area for inputs from lateral, median, and ventral eyes. Scattered neurohemal terminals face circulatory sinusoids within the brain. Efferent fibers, originating from unspecified, presumably neurosecretory cells in the central nervous system, course through the optic nerves and terminate on pigment and receptor cells of the eyes. Their terminals have a synaptoid structure and their granules have the characteristic density and crystalline substructure of neurosecretory granules. Transection of the lateral optic nerve leads to degranulation and gradual disappearance of terminals and to accumulation of granules at the proximal ends of cut fibers. The Limulus ocular innervation presents a striking example of directed delivery of neurosecretion to pigmentary effector cells, whose adaption is usually controlled by circulating neurohormones.
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  • 22
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    Cell & tissue research 144 (1973), S. 309-324 
    ISSN: 1432-0878
    Keywords: Pericardial organ ; Anterior ramifications ; Neurosecretion ; Electron mikroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pericardial organ-anterior ramifications complexes of Uca pugilator and Callinectus sapidus were studied by transmission electron microscopy. Five morphologically distinguishable groups of granules and two groups of vesicles were identified. These granules and vesicles are present in approximately the same proportions in the pericardial organs and anterior ramifications of both species. Two of the granule groups are never mixed in the same axon terminals and are believed to represent different hormone-protein complexes. The remaining granule and vesicle groups are believed to be products of neurosecretory hormone release. Evidence that at least some of these granules and vesicles arise from intraaxonal release of neurosecretory material is presented.
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  • 23
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Neurophysin ; Diabetes insipidus ; Vasopressin
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    Notes: Summary The subcellular distribution of radioactive neurophysin and the release of neurophysin from isolated neurosecretory granules (NSG) from the posterior pituitary gland were studied at various time intervals after injection of (35S) cysteine into the supraoptic nucleus under various functional conditions. In consistency with previous findings (Norström, 1972), increased amounts of radioactive neurophysin were recovered extra-granularly at longer time intervals after isotope injection. Increased quantities of “free” labelled neurophysin were found in lactating rats and homozygous diabetes insipidus (DI) rats 7 and 1 day after injection, respectively. Salt-loading for 4 days did not affect the subcellular distribution of radioactive neurophysin 1 day after injection of (35S) cysteine. 8.5 days after isotope injection acute haemorrhage was followed by augmented amounts of radioactive neurophysin among the soluble proteins, but at 1 day no difference was observed between bled animals and controls. The “spontaneous” release of neurophysin form NSG was higher in stimulated animals and increased with time after isotope injection in controls as well as in experimental animals. The absolute increase in release of neurophysin in response to ATP was higher in stimulated rats whereas NSG from DI rats did respond poorly to ATP.
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    Cell & tissue research 142 (1973), S. 443-464 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Posterior Pituitary gland ; Colchicine ; Axoplasmic flow ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of colchicine on the release of neurosecretory material from the posterior pituitary gland was investigated in the rat in vivo and in vitro. Colchicine was administered subarachnoidally when neurophysin, radiolabelled by injection of (35S) cysteine into the supraoptic nucleus, had accumulated in the neural lobe. Dehydration for 3 days of non-colchicine-treated rats was followed by a 100% reduction of neurophysin-bound radioactivity. When colchicine was given prior to dehydration, the reduction of radioactive neurophysin was less marked. Colchicine treatment alone was likewise followed by a lowering of protein-bound radioactivity in the neural lobe, which may indicate that colchicine, in addition to blocking the rapid axonal transport of neurosecretory material, also impedes the slow transport. The release of radioactive neurophysin in response to depolarizing concentration of potassium in vitro was diminished in the presence of colchicine, the reduction being most pronounced after colchicine treatment in vivo. The biochemical data prove the view that colchicine inhibits the release of neurosecretory material from the neural lobe. The ultrastructural findings support the biochemical data. Thus, colchicine treatment alone or followed by dehydration induced a marked increase in the number of organelles, especially of mitochondria and dense bodies. There was a marked increase in the number of enlarged axons filled with dammed organelles in the infundibulum and neurohypophysis. There was an accumulation of dense core vesicles and microvesicles in the axonal terminals in the neurohypophysis after treatment with either colchicine or colchicine followed by dehydration, which indicates an impediment of the release process. Dehydration alone induced a depletion of the dense core vesicles in the terminals. Out from the combined biochemical and ultrastructural findings possible mechanisms for the action of colchicine are discussed.
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  • 25
    ISSN: 1432-0878
    Keywords: Axonal transport ; Neurosecretion ; Posterior pituitary ; Anesthetics ; Chlorpromazine ; Electron microscopy
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    Notes: Summary The effects of chlorpromazine (cpz) and tetracaine (tc) on the rapid axonal transport of neurosecretory material (NSM) in the hypothalamo-neurohypophysial system was investigated. Following subarachnoidal injection of these drugs, the incorporation of (35S) cysteine into proteins of the supraoptic nucleus was slightly depressed. The protein-bound radioactivity in the posterior pituitary was markedly lowered in experimental rats which indicates a partial blockage of the rapid axonal transport of NSM along the hypothalamoneurohypophysial tract. Both cpz and tc induced an increase in the number of mitochondria and profiles of granular endoplasmic reticulum. The axons in the infundibulum and neurohypophysis were enlarged by dammed organelles, indicating a blockage of axonal transport. There was an increased number of microvesicles, often arranged in a crystalloid pattern, in the terminals. The number and distribution of neurotubuli and neurofilaments were not changed. A possible stimulatory effect of cpz on the release of NSM from the neural lobe is assumed Possible mechanisms for the action of mitotic inhibitors, transquilizers and local anesthetics are discussed.
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  • 26
    ISSN: 1432-0878
    Keywords: Neurophysin ; Neurosecretion ; Axonal transport ; Immunohistochemistry
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    Notes: Summary 1. An immunohistochemical study has been made of the hypothalamo-neurohypophyseal system of the dog, 20h after crushing the pituitary stalk. 2. By use of a cross-species-reactive neurophysin antiserum it was shown that neurophysin is a component of the axons which originate in the supraoptic and paraventricular nuclei and terminate around blood vessels in the posterior pituitary. 3. Neurophysin specific fluorescence accumulated in axons proximal to the constriction but was absent from the axons immediately distal to the site of injury. 4. In dogs left for six days it was shown by radioimmunoassay that the amount of neurophysin in the hypothalamus and stalk proximal to the constriction increased twofold while that remaining in the posterior pituitary and stalk distal to the constriction decreased five-fold over the same period. 5. The results are interpreted as evidence for a rapid axonal transport of neurophysin from its site of synthesis in the cell bodies of the hypothalamus to the posterior pituitary.
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  • 27
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    Cell & tissue research 137 (1973), S. 223-250 
    ISSN: 1432-0878
    Keywords: Nervous system ; Ctenophores ; Nerve-net, Nervous concentration ; Synapses ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A subectodermal nerve-net is demonstrated in ten species of Ctenophores by means of either Methylene Blue vital staining or silver impregnation. There is no evidence of subendodermal nerve-net. The ectodermal nerve-net displays a characteristic polygonal pattern which is thought to result from morphogenetic events. Both bipolar and tripolar neurones occur. Three types of concentrations of nervous tissue are described: a) a high accumulation of nervous perikarya at the aboral sensory pole; b) a noticeable densification of the net along the eight meridional ciliary strands of every species, and around the lips of Beroidea; c) in the Cydippids Pleurobrachia and Hormiphora two thick strands of fibers and neurones interconnecting the aboral organ and the tentacles: the tentacular nerves. Ultrastructural evidence is given for the presence of nervous elements under and amidst ectodermal epithelial cells. Three features allow the recognition and characterization of neurites and neurones: a) numerous clear and/or granulated vesicles; b) microtubules in variable amounts; c) frequent and highly differentiated synaptic contacts. The very peculiar arrangement of the presynaptic elements is observed in all the species of Ctenophores so far studied. These synaptic contacts suggest chemical transmission in the nerve-net. On the basis of ultrastructural evidence the author refutes the nervous nature previously attributed to the ciliated cells of the meridional grooves. A parallel is drawn between nervous systems of Cnidaria and Ctenophora.
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    Cell & tissue research 140 (1973), S. 25-37 
    ISSN: 1432-0878
    Keywords: Heart innervation (Turtle) ; Monomamine-containing cells ; Synapses ; Fluorescence and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fluorescence and electron microscopy of the turtle heart indicates the presence of monoamine-containing, granulated cells in the wall of venous sinus and near the openings of the aortic trunks. These cells occur in clusters and are always in association with nerve fibers and/or ganglion cells within the turtle heart. Vesiculated axon terminals make a synaptic contact with the cytolemma of the granulated cell which in turn makes a synapse to the processes from the other granulated cell and to the cardiac nerve fibers. The close contact occurs also between the granulated cell process and the smooth muscle cell membrane in the wall of large vessels. The granulated cell has no special relationship to the blood capillaries. The significance of these results is discussed in relation to nervous control of the cardiac activity of the turtle.
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  • 29
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    Cell & tissue research 140 (1973), S. 203-216 
    ISSN: 1432-0878
    Keywords: Synapses ; Development ; Chick embryo ; Cell culture ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary The formation and development of synaptic contacts between dissociated chick spinal cord neurons has been investigated. By the 6th day in vitro “immature” profiles with few vesicles were observed. By 14–18 days “mature” types with numerous vesicles were found, indistinguishable from those of newly hatched chick spinal cord. After this period degeneration occurred, and was especially marked in the post-synaptic element. Such degeneration could be postponed by the addition of small numbers of somatic muscle cells. The Kanaseki and Kadota (1969) technique was applied to the study of coated vesicles at various stages of synaptic development.
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    Cell & tissue research 142 (1973), S. 329-345 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Midbrain ; Cyclostomata ; Lamprey, female ; Light- and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Description / Table of Contents: Zusammenfassung Im Torus semicircularis des Mittelhirns der Neunaugen lassen sich sekretorische Neurone nachweisen. Das mesenzephale neurosekretorische Zentrum entwickelt sich erst nach der Metamorphose und ist nur bei den weiblichen Tieren ausgebildet. Das Neurosekret ist ein zystinreiches Protein und läßt sich im histologischen Schnittpräparat mit allen Methoden, die zum Nachweis peptiderger Neurosekretion im Hypothalamus verwendet werden, darstellen. Produktions- und Speicherorte des Neurosekrets sind erweiterte Zisternen des rauhen endoplasmatischen Retikulums. Es existieren keine speziellen Speicherorganellen in Form der bekannten runden, einheitlich elektronendichten Elementargranula oder in Form von dense-core Vesikeln. Speicherzisternen konnten auch in den Fortsätzen der neurosekretorischen Zellen nachgewiesen werden.
    Notes: Summary In the torus semicircularis of the midbrain of lampreys a neurosecretory area was found. The mesencephalic neurosecretory nucleus develops not earlier than after metamorphosis and is only present in females. The neurosecretory material could be determined as a cystine-rich protein demonstrable in slices, with all of the methods, that are employed for the demonstration of hypothalamic neurosecretion. The neurosecretion is produced and stored in dilatated cisterns of the rough endoplasmic reticulum. No specialized storing vesicles in the form of the well-known spherical, uniformly electron-dense elementary vesicles or dense-core vesicles are present. The storing cisterns could also be demonstrated in the processes of the neurosecretory cells.
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    Cell & tissue research 146 (1973), S. 367-374 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neuromuscular junction ; Synapse, Synaptoid ; Insect
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    Notes: Summary The axons which innervate the ventral abdominal intersegmental muscles ofRhodnius prolixus make typical synaptic contacts with the muscles but are also neurosecretory. The neuromuscular junctions are characterised by the close apposition of the plasma membrane of the axon and that of the muscle, and also by the presence of synaptic vesicles in the axons. Every axon innervating the muscle contains neurosecretory granules and synaptoid structures. The neurosecretory granules are concentrated in groups along the axons, and appear to be released by exocytosis both locally around the muscles and generally into the haemolymph. The synaptoid structures consist of groups of electron-lucent vesicles which are usually clustered against the axolemma at sites adjacent to the haemolymph.
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    Cell & tissue research 146 (1973), S. 1-14 
    ISSN: 1432-0878
    Keywords: Ventral cord ganglia ; Limulus polyphemus ; Cell types ; Neurosecretion ; Cytochemistry ; Light microscopy
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    Topics: Biology , Medicine
    Notes: Summary The ventral cord ganglia of the horseshoe crab, Limulus polyphemus, contains six distinct cell types: three appear to be ordinary neurons and three exhibit the staining affinities of neurosecretory cells. The presumed neurosecretory cells have been termed neurosecretory cell I (NSC I), NSC II and NSC III. NSC I cells contain a colloid-like inclusion which may occur as a single small vacuole or occupy more than one-half of the cell volume. Colloid inclusions occur with greater frequency toward the periphery, although small cells of similar staining affinity occur in cords extending to the fibrous core. The histochemical tests suggest that the cytoplasm is positive for proteins, but contains no strong acidic groups which may have been derived from S-S or S-H groups. The presence of carbohydrate is also indicated. NSC II cells exhibit distinct secretory cycles. Early in the cycle the cytoplasm becomes phloxinophilic and progresses to a distinct fuchsinophilic stage. Small homogeneous irregular inclusions are found in the axon hillock during the latter stages of the cycle. Histochemical tests suggest the presence of a carbohydrate and strong acidic groups which may have been derived from S-S or S-H groups. There are small cells present which appear to be immature neurosecretory cells. NSC III cells are characterized by a perinuclear ring of cytoplasm which is stained by chrome alum hematoxylin but not by paraldehyde fuchsin. A secretory cycle may also be present in this cell type. The three cell types presumed to be ordinary neurons exhibit no particular staining affinity for the stains or tests used in this study.
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    Cell & tissue research 123 (1972), S. 47-54 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Exocytosis ; Electron microscopy
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    Description / Table of Contents: Zusammenfassung 1. Elektronenmikroskopische Untersuchungen an der Neurohypophyse von Ratte und Forelle ergeben, daß sich eine Exocytose von Elementargranula an den Endigungen der neurosekretorischen Fasern nur selten abspielt. Es wird daher angenommen, daß die Abgabe von Hormonen in der Neurohypophyse in der Regel nach dem Muster des „membrane-release“ abläuft. 2. Die Exocytose wird nicht durch eine unmittelbare tangentiale Fusion der Membran des Elementargranulums mit dem Plasmalemm der Nervenendigung (Axolemm) eingeleitet. Vor allem bei Anwendung eines Goniometertisches wird erkennbar, daß vor der Exocytose zwischen Axolemm und Membran des Granulums eine Verbindung in Gestalt eines Stieles entsteht. Die Länge dieses Verbindungsstückes entspricht etwa 2 Axolemmdicken. An der Basis des Stiels im Axolemm tritt das Stoma auf, durch das der Inhalt des Granulums bzw. dieses selbst das Axonende verläßt. 3. Die Herkunft kleiner membrannaher Vesikel (Durchmesser 500 Å) in den Endigungen neurosekretorischer Nervenfasern in der Neurohypophyse konnte nicht geklärt werden. Anzeichen einer kompensatorischen Endocytose im Sinne von Nagasawa, Douglas und Schulz (1970) wurden nicht beobachtet.
    Notes: Summary 1. Electron microscopical investigations of the neurohypophysis in rat and trout reveal that exocytosis of neurosecretory elementary granules from the nerve endings occurs only rarely. The authors are of the opinion that hormone release in the neural lobe follows mainly the “membrane-release” pattern. 2. Exocytosis is not performed by tangential fusion of the elementary granule membrane and the plasmalemma of the nerve ending (axolemma). Administering the goniometer technique one can observe the appearance of a stalk-like structure connecting the two membranes. The basis of the stalk in the axolemma corresponds to the site of the stoma through which the core of the vesicle leaves the nerve ending. 3. The mechanism of the origin of small clear vesicles (diameter 500 Å approx.) near the axolemma of the neurosecretory terminal has not been elucidated. The authors did not observe equivalents of a compensatory endocytosis in the vicinity of granules released by exocytosis.
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  • 34
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    Keywords: Neurones ; Lumbricus ; Monoamines ; Neurosecretion ; Electron microscopy
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    Notes: Summary The cerebral ganglion and the ventral nerve cord of Lumbricus terrestris have been studied with the electron microscope. The results are as follows: In the neuropile small granular vesicles (300 to 500 Å) occur in some varicose nerve fibres after fixation with potassium permanganate. This indicates the presence of noradrenaline. Sometimes only a few of the vesicles produce a positive reaction. After incubation with α-methyl-noradrenaline the numbers of nerve terminals with small granular vesicles greatly increase, indicating the presence of dopamine and/or 5-hydroxytryptamine. In this case the reaction is now complete. The number of small granular vesicles is largest in the terminal swellings. These findings are consistent with histofluorescence, chemical, and microspectrofluorometric analyses, which have demonstrated noradrenaline, dopamine, and 5-hydroxytryptamine in neurones in the central nervous system. Large granular vesicles (600 to 900 Å) are to be found in some perikarya, not identical with neurosecretory cell bodies. In this case the granular vesicles in the axon are smaller and fewer. This indicates a simultaneous proximo-distal transport and gradual decrease in size of the granular vesicles. The intraneuronal distribution of the vesicles is in agreement with the distribution of the fluorophores in the fluorescent neurones. Neurosecretory neurones are found most likely not to contain monoamines.
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    Cell & tissue research 127 (1972), S. 240-257 
    ISSN: 1432-0878
    Keywords: Insects ; Brain ; Corpora pedunculata ; α-lobe ; Synapses
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    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Organisation der α-Loben der Pilzkörper im Gehirn von Acheta domesticus L. wird nach licht- und elektronenmikroskopischen Befunden beschrieben. Der säulenartige Faserkomplex des α-Lobus besteht aus Fortsätzen von Pilzkörperzellfasern (intrinsischen Fasern, IF) und pilzkörperfremden Fasern (extrinsischen Fasern, EF), die in den Lobus eindringen. Die feinen IF durchziehen den Lobus hauptsächlich parallel zu seiner Längsachse, während die EF zumeist senkrecht zur Längsachse angeordnet sind. Der Lobus erscheint von seiner Peripherie bis zu seinem Zentrum durch IF-Zonen gegliedert. Die Verteilung der EF weist auf eine zusätzliche Ordnung von der Basis zur Spitze des Lobus hin. Zahlreiche polarisierte Synapsen verbinden IF mit EF. Die IF zeigen Vesikelanhäufungen und präsynaptische Apparate besonders in Erweiterungen, die auch in Golgi-Präparaten lichtmikroskopisch zu sehen sind. Es werden zwei EF-Typen unterschieden: 1. Postsynaptische EF (zahlreich) und 2. EF mit prä- und postsynaptischen Kontakten, die nur in einigen Regionen des α-Lobus gefunden wurden. Präsynaptische IF konvergieren auf „dendritische“ EF, die Verbindungen mit anderen Teilen des Hirns und des Nervensystems herstellen. Funktionelle Gesichtspunkte werden diskutiert.
    Notes: Summary The organization of the α-lobes of the corpora pedunculata in the brain of the cricket Acheta domesticus L. has been investigated in the light and electron microscopes. The cylindrical fibre complex is composed of branches of “mushroom-body” fibres (intrinsic fibres) and extrinsic fibres, which penetrate the α-lobe. Intrinsic fibres (IF) run through the α-lobe in the same direction, but not strictly parallel to each other or to the axis of the α-lobe. Extrinsic fibres (EF) and their fine branches are often arranged perpendicular to the axis of the α-lobe. There is some evidence that different IF zones occur in the α-lobe when passing from its periphery to its centre. The distribution of EF may reflect a structural order when passing from the base of the lobe to its top. Numerous polarized synapses connect the IF with the EF. The IF show clusters of vesicles and presynaptic figures especially in their “blebs”, which can be seen in Golgi preparations for light microscopy. Two types of EF are distinguished on the basis of their synaptic junctions: (1) postsynaptic EF (abundant) and (2) EF with pre- and postsynaptic sites (perhaps restricted to some regions of the α-lobe). Presynaptic IF converge on EF, which may transfer excitation from the α-lobe to different parts of the brain and nervous system.
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  • 36
    ISSN: 1432-0878
    Keywords: Synapses ; Crustacea ; Abdominal Ganglia ; Lateral glant fibers ; Electron microscopy
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    Notes: Summary The abdominal ganglia of the crayfish Astacus pallipes contain numerous vertebrate-like synapses which are characterized by presynaptic vesicles, darkened pre- and post-synaptic membranes, cleft material, and post-synaptic “fuzz”. Such synapses occur throughout the ganglia but are most easily found dorsally, where the neuropile is relatively coarse. The neuropile is far from homogeneous. Regional variations in fiber size, in degree of profile tortuosity, and in kind, magnitude, and distribution of vesicular content result in conspicuous textural variations. The structural polarity of synapses between the lateral giant fibers and other neurons is consistent with known physiological polarity and, hence, validates our criteria for recognition of synapses within the ganglion.
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    Cell & tissue research 134 (1972), S. 337-349 
    ISSN: 1432-0878
    Keywords: Median eminence of birds ; Thyrotrop function ; Neurosecretion ; Light-and electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary 1. The effects of TSH and PTU treatment with thyroid stimulating hormone and propylthiouracil on the structure of median eminence of the pigeon have been studied by light- and electron microscopy. 2. By light microscopy it has been found that the aldehydefuchsin positive material of the external layers of the anterior median eminence differs functionally from that of the neural lobe. 3. The light- and electron microscopic investigations revealed that the anterior and posterior median eminence differ both structurally and functionally. Specifically after the experimental interventions the considerable reaction can be observed only in the anterior median eminence. 4. Electron microscopically it appears possible that the aldehyde-fuchsin positive material of the anterior median eminence may be associated with the 1200–1500 Å—size granules. 5. The quantitative changes observed in the granular composition after the treatments show a close correlation with thyroid function, namely after treatment with thyroid stimulating hormone the relative quantity of the 1200–1500 Å—size granules increases markedly, while administration of propylthiouracil has the opposite effect and increases the relative quantity of the 1000 Å—size dense-core vesicles. They presumably may play a role in the control of TSH-secretion.
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    Cell & tissue research 132 (1972), S. 25-34 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Perivascular cells ; Neurosecretion ; Ultrastructure
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    Topics: Biology , Medicine
    Notes: Summary The purpose of this study is to investigate the ultrastructural features of perivascular cells as found in the neural lobe of the hypophysis. In particular, an inquiry was made into the nature of the relationship of such cells to neurosecretory fibers and endings. The latter, in fact, are often invaginated within the perivascular cells and enveloped by their processes; furthermore, they often reveal a certain number of empty granules as well as characteristics of degenerative nature. In the course of this study the localization of the perivascular cells has been investigated as well as that of their processes within the extensive interlobular network typical of the hypophysial neural lobe of rodents. Based on the data gathered, the hypothesis is put forward that the perivascular cells play an important role in the turnover of neurosecretory endings, both under physiological and experimental conditions, contributing thereby to the release of post-hypophysial hormones.
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    Cell & tissue research 125 (1972), S. 415-431 
    ISSN: 1432-0878
    Keywords: Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic junctions in intact rat cerebral cortex have been examined following glutaraldehyde fixation and phosphotungstic acid (PTA) staining. In the presynaptic ending the network has a hexagonal arrangement, while the dense projections are regularly placed along the presynaptic membrane. Cleft densities occupy the intracleft region. The postsynaptic thickening extends uninterrupted along the length of the junction. Qualitatively, the majority of junctions fall into the ‘discontinuous-continuous’ category, in which the internal coat of the presynaptic membrane together with its associated dense projections is discontinuous along the length of the junction, whereas the postsynaptic thickening is continuous. By contrast, a small number of junctions are ‘continuous-continuous’. In an attempt to analyze the junctions quantitatively, nine indices were measured. Histograms of the size distributions of seven of these appear to be bimodal, and from this it is concluded that two junction populations may be distinguishable on quantitative grounds. It is also shown that the distance separating dense projections at the presynaptic membrane is of the order of 10–15 nm. This surprisingly low value has consequences for current ideas on the relationship between synaptic vesicles and dense projections, and these are discussed at length.
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    Cell & tissue research 125 (1972), S. 432-447 
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Working with glutaraldehyde fixed, PTA stained rat cerebral cortex, the authors compared the ultrastructural features of synaptosomes with those of intact synaptic junctions. In general there is close correspondence between the two, although the cleft densities and postsynaptic focal densities of synaptosomes show a greater degree of focalization than their counterparts in synaptic junctions. The dense projections have similar profiles in both preparations, but are more difficult to distinguish clearly in synaptosomes on account of the closer packing of the presynaptic network around their apices. The limiting membrane of the presynaptic terminal is usually visible in synaptosomes, but not in synaptic junctions. Comparing the preparations quantitatively reinforces the qualitative findings, and points to their overall similarity. However a number of the indices in synaptosomes are significantly smaller than the corresponding ones in synaptic junctions, and this points to the operation of a shrinkage factor during fractionation procedures. This is confined to the pre- and post-synaptic components and does not affect the intervening contact region. Histograms of the size distributions of the indices are similar to those obtained for intact synaptic junctions, the majority displaying two peaks. It is concluded that synaptosomes accurately reproduce the major ultrastructural features of synaptic junctions.
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  • 41
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    Cell & tissue research 127 (1972), S. 189-200 
    ISSN: 1432-0878
    Keywords: Crustacean ; Muscle ; Nerve ; Blood vessel ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to study the innervation and vascular supply of crayfish skeletal muscle. Blood vessels and nerve terminals identified by TEM were often closely associated. Synaptic regions of the nerve terminals were always located under sarcolemma and contained both dense-cored and agranular synaptic vesicles. Axo-axonal synapses of several different types were observed. Blood vessels consisted of several “vessel cells” or “supporting cells” enclosing a lumen, which was connected to the exterior by fine channels between the “supporting cells”. SEM of whole freeze-dried muscles revealed two types of ramifying structure, which often ran in parallel over the muscle surface. One, identified as nerve, was more cylindrical and had a smoother surface than the other, which was identified as blood vessel. Fine nerve branches disappeared under the sarcolemma, probably near synaptic regions, but synapses could not be seen. Blood vessels also had fine terminations which merged into the sarcolemma.
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  • 42
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    Cell & tissue research 130 (1972), S. 362-377 
    ISSN: 1432-0878
    Keywords: Lorenzinian ampullae ; Polyodon spathula ; Sensory epithelium ; Synapses ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron microscopic observations on the ampullary organs of Polyodon spathula (Chondrostei, Osteichthyes) reveal a sensory epithelium similar to that found in the Lorenzinian ampulla, an electroreceptor found in marine Elasmobranchs. The sensory cells have a very small luminal part provided with a cilium. They are innervated by many nerve endings. Each nerve fibre apparently makes synaptic contact with several sensory cells. The synaptic structure in the sensory cell is composed of a flat sheet, the outermost part of which is surrounded by 3 or 4 annuli of densely staining material. The sheet extends into a protrusion of the sensory cell, and there is a corresponding invagination in the nerve terminal. The conclusion that these organs are electroreceptors, is supported by the finding that the fish responds to the introduction of an iron tube in the aquarium, whereas a wooden rod introduced in the same way causes no response.
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  • 43
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    Cell & tissue research 130 (1972), S. 481-488 
    ISSN: 1432-0878
    Keywords: Limulus heart ; Neurogenic heart ; Neuromuscular junctions ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural characteristics of the neuromuscular junctions were studied in the neurogenic heart of Limulus polyphemus. Several types were encountered. The first type consisted of nerve terminals which synapsed along the borders of the myocardial fibers, embedded just under the sarcolemma. A second type of terminal was ensheathed in glial cells, synapsing on the outer sarcolemmal membrane. The third, and most prevalent type of junction consisted of terminals which synapsed with arms of granular sarcoplasm, remote from the fibrillar portion of the muscle fibers. Junctional complexes of the third type were often observed near intercalated discs and were often formed by several axons synapsing with arms of sarcoplasm from several muscle fibers. The results are discussed in relation to the previously reported electrophysiological characteristics of the neuromuscular junctions.
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  • 44
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    Cell & tissue research 129 (1972), S. 92-113 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Neurosecretory granule ; Rat ; Membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurosecretory granules (NSG) of rat posterior pituitary glands were prepared by differential centrifugation techniques mainly according to the procedure as described by Barer, Heller and Lederis (1963). As revealed by electron microscopy, the recovery of neurophysin and the contents of enzymes, purified NSG were obtained in a pellet at 30 000 g/60 min (0.44 M sucrose). Eighteen h after injection of (35S) cysteine into the supraoptic nucleus 60% of the recovered radioactivity in the neural lobe was found in the NSG, whereas 20% was found in the final supernatant (100 000 g/120 min). Sixteen days after injection the NSG and the final supernatant fraction contained fairly equal amount of (35S) cysteine (approximately 40%). It is suggested that after a period of intragranular maturation neurophysin is extruded into an extragranular pool of neurosecretory material. With the use of conventional polyacrylamide-gel electrophoresis it was shown that the predominating proportion of radioactivity in the NSG after a hypothalamic injection of (35S) cysteine was located within the neurophysin fraction A and in fraction B. Fraction B is suggested to be partly bound to the NSG membranes. When the NSG soluble and NSG insoluble proteins, obtained after lysis of NSG, were separated on polyacrylamide gels in the presence of sodium dodecylsulphate, the highly radioactive soluble protein was shown to consist of two components with average molecular weights of 12 300 and 14 600. Most of the proteins in the lysate were found in the NSG membranes, though less radioactive. A component with a mol.wt. of 37 000 was enriched in the membrane fraction. At longer times after isotope injection the high mol.wt. proteins, particularly those of the NSG membranes, contained increased amounts of radioactivity.
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  • 45
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    Cell & tissue research 134 (1972), S. 383-402 
    ISSN: 1432-0878
    Keywords: Nucleus praeopticus ; Xenopus, tadpole ; Neurosecretion ; Osmoregulation ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the preoptic nucleus of Xenopus laevis tadpoles during metamorphosis was studied and the effect of osmotic stimulation on this process investigated. The development of this region was not affected by treatment for one or more days in hypertonic media. It was found that at the end of metamorphosis the neurosecretory cells in the preoptic nucleus are localized in three regions: the rostro-dorsal, the caudo-dorsal and the ventral region. After osmotic stimulation only the neurosecretory cells of the caudo-dorsal region appeared to have reacted, as indicated by their loss of neurosecretory (PIC positive) material. It is concluded that the cells of this region may be involved in the synthesis of the posterior lobe hormones.
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  • 46
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    Cell & tissue research 126 (1972), S. 278-296 
    ISSN: 1432-0878
    Keywords: Anura ; Pineal organ ; Synapses ; Acetylcholinesterase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In der Epiphyse von Bombina kommen durch „synaptic ribbons“ gekennzeichnete Synapsen und konventionelle Synapsen vor. Bei den „ribbon“-Synapsen handelt es sich um axodendritische und axosomatische Formen. Die axodendritischen „ribbon“-Synapsen lassen sich aufgrund der Zahl der Dendriten und der „synaptic ribbons“ in 2 Typen gliedern. Es kommen Dendriten vor, die nacheinander in „ribbon“-Synapsen und konventionelle Synapsen einbezogen sind. Neben konventionellen und durch „ribbons“ gekennzeichneten synaptischen Verbindungen finden sich weitere Kontakte zwischen Sinnes- und Nervenzellen und Interrezeptorkontakte, die jedoch beide nicht als echte Synapsen angesprochen werden können. Anhand der Befunde zur Synaptologie werden Probleme der neuronalen Schaltung der Epiphyse diskutiert. Beim Acetylcholinesterase-Nachweis findet sich das Reaktionsprodukt vor allem in den Neuropilzonen der Epiphyse. Eine eindeutige Zuordnung zu Fortsätzen bestimmter Zelltypen ist nicht möglich. Das Ergebnis des Acetylcholinesterase-Nachweises in der Epiphyse wird mit entsprechenden Befunden in anderen Bereichen des ZNS und in der Netzhaut verglichen.
    Notes: Summary Both conventional and ribbon synapses occur in the pineal organ of Bombina. Ribbon synapses are both axodendritic and axosomatic. Two axodendritic types can be distinguished on the basis of the number of dendrites and synaptic ribbons. Both conventional and ribbon synapses can be formed with the same dendrite. Other contacts, which cannot be classified as true synapses, are also found between sensory cells and nerve cells and likewise between sensory cells. The synaptology of the pineal organ permits a discussion of the problems of its neurocircuitry. Products of the acetylcholinesterase reaction occur mainly in the plexiform layer of the pineal organ. It is not possible to correlate the reaction products with definite cell types. The results of the acetylcholinesterase reaction in the pineal is compared with corresponding findings in other parts of the CNS and in the retina of the lateral eyes.
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    Cell & tissue research 129 (1972), S. 114-139 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Pituitary gland ; Rat ; Neurophysin ; Vasopressin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The release of neurophysin from neural lobe tissue slices and isolated neurosecretory granules of the rat was studied at various time intervals after injection of (35S) cysteine into the supraoptic nucleus. For hours after isotope injection the release of radioactive neurophysin from neural lobe tissue was increased by depolarizing concentration of potassium in the presence of calcium ions. Fourteen and 30 days after isotope injection the release of radioactive neurophysin was relatively decreased in a medium of high potassium concentration which might be explained by the heterogeneity of the pool of neurophysin within the neural lobe. Four h after isotope injection the “spontaneous” release of neurophysin from neural lobe tissue was higher in dehydrated rats than in controls, and the neural lobes of these animals did not respond with an increased release of radioactive neurophysin when exposed to high potassium concentration. Eighteen h after isotope injection the predominating proportion of neurophysin-bound radioactivity was found in the neurosecretory granule fraction, whereas 14 days after injection a fairly equal amount of radioactivity was found in this fraction and in the “soluble” protein fraction. This indicates that with time an increasing amount of radioactive neurophysin passes from an intragranular to an extragranular pool. The spontaneous release of radioactive neurophysin from isolated neurosecretory granules was higher, and the increase of release upon exposure to an ATP-regenerating system was higher 14 days after isotope injection than 18 h after injection. This may imply that the neurosecretory material undergoes an intragranular “maturation” process to become more easily releasable. The release of radioactive neurophysin was inhibited in the presence of AMP and EDTA, which demonstrates the dependence of the release process of an ATPase and, probably, of calcium ions. Neurosecretory granules from dehydrated rats possessed a higher spontaneous release than those of control rats 18 h after injection, which may indicate an enhanced intragranular “maturation” process of the neurosecretory material due to osmotic stimulation.
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  • 48
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    Cell & tissue research 114 (1971), S. 61-72 
    ISSN: 1432-0878
    Keywords: Corpora cardiaca ; Neurosecretion ; Insects ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les corpora cardiaca de l'adulte de Locusta migratoria sont formés de deux régions bien individualisées ce qui nous a permis de reconnaître la sécrétion propre des différents types de neurosécrétion. Dans la région nerveuse, nous distinguons par la taille des grains trois types de neurosécrétion dense classique et un quatrième type d'aspect clair. Dans la région »propre« non nerveuse, les cellules ont des caractères nettement endocriniens et sont mélangées à un seul type d'axones neurosécréteurs.
    Notes: Summary The corpora cardiaca of adult Locusta migratoria consist of two well separated areas, a fact which permits the differentiation between intrinsic and extrinsic neurosecretory material. In the neural area three types of electron dense “classical” neurosecretory granules, and a fourth more lucent type can be distinguished according to size. In the non-neural “glandular” area typical endocrine cells mingle with only one type of neurosecretory axons.
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    Cell & tissue research 115 (1971), S. 494-500 
    ISSN: 1432-0878
    Keywords: Axon ; Synapses ; Astrocytes ; Thalamus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies and three-dimensional graphic reconstructions from serial sections have shown that the large axon terminals of synaptic glomeruli in the ventrobasal nucleus of the rat are invaginated by spine-like protrusions from the astrocyte processes surrounding the glomeruli (“micro-trophospongium”). The astrocytic protrusions are similar in dimensions and internal morphology to the synapse-bearing dendritic excrescences that also invaginate the large axon terminals. Consequently astrocytic protrusions may be overlooked, or confused with dendritic excrescences sectioned at a non-synaptic level. The intimate neuronal—neuroglial relationship at such large axon terminals may reflect ion-exchange or metabolic interactions between the astrocytes and the axon terminal.
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    Cell & tissue research 122 (1971), S. 283-300 
    ISSN: 1432-0878
    Keywords: Paraventricular nucleus ; Rat ; Synthesis of hormones ; Acid phosphatase ; Osmium impregnation ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural features of the paraventricular neurones of the non-treated rat are presented comparing them with those of the supraoptic neurones. No striking differences are seen between the general electron microscopic characteristics of the paraventricular and supraoptic neurones. The importance of adequate fixation to obtain good preservation of the neurones is emphasized, since inadequate fixation can cause e.g. artefactual appearance of “dark neurones”. The previously presented classification of the neurosecretory neurones into two categories (e.g. “light and dark neurones”) on the basis of the number of ribosomes is not considered justifiable, since their number can vary to a very great extent even within a single cell. The synthesis of neurosecretory products in the paraventricular neurones obviously follows the general mode of the synthesis of secretory proteins: ribosomes—RER—Golgi complex—secretory vesicles. On the basis of the localization of heavy metal deposits after osmium impregnation and demonstration of acid phosphatase the Golgi complex of the paraventricular neurones is found to be polarized. The direction of the polarity is discussed. The substructures of the dense cores of the neurosecretory granules and of the contents of the lysosomal dense bodies are nearly identical. Therefore it is considered impossible to determine positively the nature of the dark condensed material within the Golgi complex. The characteristics of the immature neurosecretory granules and the possibility of releasing neurosecretory products into the cytoplasm already within the perikarya are speculated.
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  • 51
    ISSN: 1432-0878
    Keywords: Hypophysis ; Chondrichthyens ; Embryology ; Neurosecretion ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
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    Description / Table of Contents: Résumé L'identification et la répartition des cellules endocrines dans l'ébauche adénohypophysaire de Scyllium canicula, et la chronologie de la différenciation cellulaire et de l'histogenèse de cette glande ont fait l'objet de ce travail. Plus nettement encore que chez l'adulte on démontre qu'il y a quatre populations de cellules hormonogènes, localisées chacune dans un lobe différent. Les grains de sécrétion des cellules du lobe rostral sont glycoprotidiques et ont un diamètre modal, de 170 nm, ceux du lobe médian, de la même taille, sont protidiques ou polypeptidiques. Les gros granules du lobe neuro-intermédiaire (diamètre modal 260 nm) sont glycoprotidiques, les petits granules du lobe ventral (diamètre modal 120 nm), protidiques au polypeptidiques. Au stade 10 mm, les cellules de la poche de Rathke sont différenciées de celles de l'épithélium stomodéal par la présence d'inclusions énigmatiques, les ≪godd bodies≫ de Schechter. Au stade 35 mm les cellules des noyaux préoptiques et du tuber comportent des grains de neurosécrétion, de même diamètre, variant autour de 100 nm. A ce même stade, des granules apparaissent simultanément dans les quatre lobes de l'hypophyse, notamment dans le lobe neuro-intermédiaire où aucune fibre neurosécrétrice n'est encore décelée. L'innervation directe des cellules endocrines de ce lobe ne s'effectue qu'au stade 45 mm et s'accompagne de modifications de la taille des granules. La fin du développement embryonnaire se caractérise par une charge de plus en plus importante des cellules endocrines, en granules.
    Notes: Summary The identification and distribution of endocrine cells in embryonic adenohypophysis of Seyllium canicula, and chronology of cellular differentiation and histogenesis of this gland have been investigated in this work. More clearly than in the adult, the presence of four populations of endocrine cells, each one located in a different lobe, is demonstrated. Secretion granules of rostral lobe cells are glycoprotidic, their modal diameter is 170 nm; median lobe ones, of same dimensions, are protidic or polypeptidic. The nemo-intermediate lobe big granules (modal diameter: 260 nm) are glycoprotidic, the ventral lobe small granules (modal diameter: 120 nm), protidic or polypeptidic. At stage 10 mm, Rathke's pouch cells are recognized from stomodeal epithelium by enigmatic inclusions, the “odd bodies” of Schechter. At stage 35 mm, preoptic and tuberal nucleus cells include neurosecretory granules of same diameter varying about 100 nm. At this same stage, granules appear simultaneously in the four hypophyseal lobes, including the neuro-intermediate lobe where no neurosecretory fiber is present. Direct innervation of endocrine cells of this lobe is accomplished at stage 45 mm and is accompanied by changes of granules dimensions. The end of embryonic development is characterized by increasing granular load of endocrine cells.
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    Cell & tissue research 114 (1971), S. 557-579 
    ISSN: 1432-0878
    Keywords: Retina ; Tissue Culture ; Synapses ; Retinal Rods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Netzhäute von 2–3 Tage alten Ratten wurden in Plasma auf Deckgläsern in Rollerröhrchen zur Kultur angesetzt. Nach 7–17 Tagen in vitro wurden die Kulturen mit Aldehyden und Osmiumsäure fixiert und für elektronenmikroskopische Untersuchung weiterverarbeitet. Gewebsquerschnitte (senkrecht zum Deckglas) zeigten histotypische Organisation, besonders in den dickeren Abschnitten der Explantate. Die Schichtung der Zellen entwickelte sich ganz ähnlich derjenigen in der Retina in situ aus dem relativ primitiven ausgepflanzten Netzhautepithel, jedoch enthielten die verschiedenen Schichten weniger Zellen als in der Retina in vivo. Alle Hauptnervenzelltypen konnten auf Grund ihrer Lokalisation und ihrer cytologischen Merkmale unterschieden werden. Die Entstehung von membranösen Lamellen in den Außengliedern der Sinneszellen konnte als Einfaltung der Plasmamembran beobachtet werden. Synaptische Bandkomplexe in ausgereifter Form wurden in der äußeren plexiformen Schicht nachgewiesen, während konventionelle Synapsen in der inneren plexiformen Schicht häufig angetroffen wurden. Synaptische Bänder waren ebenfalls in den Axonen bipolarer Zellen in der inneren plexiformen Schicht nachweisbar. Amakrine und Ganglienzellen waren in diesen Regionen ziemlich selten vertreten. Da die Untersuchung von nicht kultivierten Netzhäuten drei Tage alter Tiere keinerlei Synapsen zeigte, wird geschlossen, daß die Synapsen in den Kulturen in vitro entstanden sein müssen. Die Netzhaut stellt ein günstiges Modell für die Synaptogenese in vitro dar, indem sie verschiedene Vorzüge vor Explantaten aus anderen Regionen des Zentralnervensystems aufweist, nämlich eine klare Schichtung, zahlreiche identifizierbare Zellfortsätze mit charakteristischen synaptischen Beziehungen und eine wohl definierte Folge von Entwicklungsvorgängen.
    Notes: Summary Retinae from two- and three-day-old rats were explanted in plasma clots and grown in vitro with the flying coverslip method. After seven to seventeen days in culture, the retinal tissue was fixed with aldehydes and osmium tetroxide and embedded for examination with the electron microscope. Study of cross sections (perpendicular to the coverslip) revealed a histotypic pattern of organization, especially in the thicker regions of the explants. Layering of cells quite similar to that in the intact retina was seen to develop from the relatively primitive, explanted retinal epithelium. However, each layer contained fewer cells than its counterpart in vivo. All major neuronal cell types were distinguished by their location and cytological characteristics. Development of the saccules of sensory cell outer segments was observed to occur in vitro by an infolding of the plasma membrane. Synaptic ribbon complexes developed to the mature form in the outer plexiform layers, while conventional synapses were numerous in the inner plexiform layers. Synaptic ribbons were also seen in bipolar cell axons in the inner plexiform layers. Amacrine and ganglion cells in these regions were relatively sparse. A survey of posterior regions of noncultured three-day-old rat retinae showed no synapses of any sort; therefore the synapses in the cultures formed in vitro. The retina is recommended for studies of synaptogenesis in tissue culture, for it offers several advantages over expiants from other areas of the neuraxis, including a clear layering pattern, many identifiable cell processes with characteristic synaptic relationships between them, and a well-defined sequence of developmental events.
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  • 53
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neuromuscular Junction ; Synaptoid ; Insect ; Frog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurosecretory nerve-endings are found in association with the ventral longitudinal muscles of the body wall in locust, stick insect and blowfly larva, and with toe muscles in the frog. In the stick insect the endings are less intimately associated with the muscles than in the locust or in the blowfly larva. In the locust and the stick insect and in the frog there are separate “neurosecretory” and “ordinary” motor endings but in the blowfly larva the characteristic features of motor and neurosecretory endings are combined in the same axon. The apparent sites of release of neurosecretory material show a characteristically rippled plasma membrane and are covered by only a flimsy layer of connective tissue material (stroma). In all three insects studied most of the neurosecretory material appears to be liberated into the haemolymph. Neurosecretory material appears to be released through the membrane at any point however, including, in the locust and in the blowfly larva, that part applied to the muscle. In the stick insect no endings have been seen as closely associated with the muscle as in the other two species. In the frog, as in the stick insect, the neurosecretory material is not liberated directly on to the muscle but into the connective tissue that lies between muscle fibres.
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  • 54
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    Cell & tissue research 118 (1971), S. 532-554 
    ISSN: 1432-0878
    Keywords: Glomera coronaria ; Cat ; Chemoreceptor cell ; Blood supply ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Gefäße der zwischen Aorta ascendens und Truncus pulmonalis, an der Vorder- und Hinterwand des Truncus pulmonalis gelegenen Glomera, sowie das sogenannte „Glomus pulmonale“ der Katze lassen sich von den Coronararterien aus durch Tusche-Gelatine-Lösungen füllen. Die erwähnten Paraganglien sind als Chemoreceptorenfelder dem Coronarkreislauf angeschlossen und daher als Glomera coronaria zu bezeichnen. Licht- und elektronenmikroskopische Untersuchungen ergaben eine morphologische Übereinstimmung mit dem Glomus caroticum. Vagusdurchtrennungen verursachen eine Degeneration der an den Glomuszellen gelegenen synaptischen Formationen sowie Veränderungen in Glomuszellen.
    Notes: Summary The vessels of the glomera that lie between the aorta and the pulmonary trunk, on the anterior and posterior wall of the pulmonary trunk, as well as the so-called glomus pulmonale of the cat have been injected with gelatine-india ink mixture. The paraganglia mentioned above are associated with the coronary circulation as chemoreceptors and are designated as the glomera coronaria. The investigations demonstrate a morphologic similarity with the glomus caroticum. Section of the vagus causes a degeneration of the synaptic structures on the glomus cells as well as changes in the cells themselves.
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    Cell & tissue research 113 (1971), S. 344-360 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Pulmonates ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Durch Anwendung von Pseudoisocyaninchlorid lassen sich im zentralen Nervensystem von stylommatophoren Pulmonaten in allen Schlundringganglien Neurosekretzellen fluoreszenzmikroskopisch nachweisen. In den Cerebralganglien bilden sie mit ihren ausleitenden Axonen das bereits bei einigen Gastropoden bekannte geschlossene neurosekretorische System. In den übrigen Ganglien liegen die neurosekretorischen Zellen einzeln oder in Gruppen zusammen und bilden kein System. Es konnte nachgewiesen werden, daß in Visceral-Parietaganglien der untersuchten Arten die meisten sekretorischen Neurone vorkommen. Die Zahl der neurosekretorischen Zellen in den einzelnen Ganglien ist zumindest für die hier untersuchten Arten wahrscheinlich artspezifisch, ihre topographische Lage in den Ganglien aber bei allen Arten identifizierbar ähnlich. Bei allen untersuchten Pulmonaten fanden sich nur im Procerebrum keine neurosekretorischen Zellen. Die vielfältigen, über die Darstellung der Neurosekretzellen hinaus feststellbaren fluoreszenzmikroskopischen Erscheinungen in den Nerven- und Gliazellen, in der Fasermasse der Ganglien, den Kommissuren und Nerven und im Bindegewebe um die Ganglien, wurden mit Hilfe anderer lichtmikroskopischer Färbungen vergleichend untersucht; die Ergebnisse werden diskutiert.
    Notes: Summary Using fluorescence microscopy with pseudoisocyaninchloride one can demonstrate the well known cells and axons of the neurosecretory system in the cerebral ganglia of some gastropod pulmonates. In the other ganglia of the central nervous system-except procerebrum-single secretory neurones or groups of neurosecretory cells occur. These secretory neurones do not build up a neurosecretory system. The majority of these cells is concentrated in the visceral-parietal ganglia. The specific elements are topographically identifiable relative to each other in the ganglia of each species of pulmonates investigated. Other fluorescence-microscopic investigations of the neurones, glial cells and the neuropil of the ganglia, commissures and nerves and in the connective tissue surrounding the ganglia are described in comparison with other light microscopical findings obtained by staining methods; the results are discussed.
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  • 56
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    Cell & tissue research 113 (1971), S. 396-419 
    ISSN: 1432-0878
    Keywords: Lateral geniculate nucleus ; Synapses ; Interneuron ; Transneuronal degeneration ; Monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two neuron types are distinguished by electron microscopy in the lateral geniculate nucleus (LGN) of the monkey-a large cell (P cell) interpreted as a geniculostriate relay cell, and a small cell (I cell) interpreted as an inhibitory interneuron. The I cell, distinguished by its small size, infolded nucleus, small mitochondria, cilium and small granular bodies, forms about 10% of the total neuron population. It could not be determined whether this cell has an axon, but its dendrites, which contain aggregates of flattened vesicles, are thought to form a proportion of the “F processes”, profiles which are post-synaptic to the retinal (RLP) axons and presynaptic to the dendrites of the P cells. The small dark (RSD) axon terminals of unknown origin contact the dendrites of both cell types. After eye enucleation the P cells of the affected laminae of the LGN shrink and partially withdraw their dendrites from the neuropil. By 29 months' survival, they have only a narrow cytoplasmic rim around the nucleus. A necrotic process also occurs, affecting fine dendrites by 22 days and large profiles by 45 days, but it is not clear whether whole cells are destroyed by this process. At 45 days the I cells are commonly seen to form somatodendritic synapses. The appearance of these synapses is interpreted as the result of a withdrawal to the soma of the presynaptic dendrites. It is concluded that the I cells are probably inhibitory interneurons subject to excitation and presynaptic inhibition by the RLP and RSD axons, and a diagram is presented to demonstrate the possible significance of these connections for the transmission of information through the LGN.
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  • 57
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    Cell & tissue research 114 (1971), S. 95-105 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Puromycin ; Annelids (oligochetes)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Untersucht wurde die Wirkung von Puromycin auf die neurosekretorischen Q- und P-Zellen im Cerebralganglion von Enchytraeus. Die ersten Erscheinungen treten bereits 15 min nach Applikation des Antibiotikums an den Nucleolen (periphere Auflockerung) und an den Ribosomen (Lösung von den Membranen) auf. In späteren Stadien der Puromycineinwirkung wurden Schwellung der Mitochondrien und Desintegration ihrer Innenstrukturen sowie Veränderungen des endoplasmatischen Retikulums beobachtet. Die durch Puromycin im Kern und Cytoplasma der Q- und P-Zelle hervorgerufenen Effekte entsprechen sich weitgehend. Die Befunde zeigen, daß die durch Puromycin (und wohl auch durch andere Antibiotika) ausgelösten substrukturellen Veränderungen meistens sehr komplexer Natur und daher nur selten auf direkte Ursachen zurückzuführen sind.
    Notes: Summary The influence of puromycin on the neurosecretory Q and P cells of the brain of Enchytraeus was studied. The first effects were noticeable in nucleoli and ribosomes as early as 15 min after the administration of the antibiotic. At later stages of puromycin treatment swelling of mitochondria and disintegration of their internal structure, as well as changes in the endoplasmic reticulum, were observed. The effects of puromycin on the nucleus and cytoplasm of Q and P cells were essentially similar. The results demonstrate that the ultrastructural changes caused by puromycin (and probably also by other antibiotics) are very complex and presumably not attributable to direct causes.
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  • 58
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    Keywords: Supraoptic nucleus ; Neurohypophysis ; Neurosecretion ; Axonal transport ; Colchicine
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The effect of colchicine on the transport of proteins in the hypothalamo-neurohypophyseal tract of the rat was studied after injection of (35S) cysteine into the supraoptic nucleus (SON) region. Colchicine, dissolved in distilled water and administered subarachnoidally, inhibited the axonal transport of labelled proteins into the neurohypophysis: the radioactivity that was recovered in neurohypophyseal TCA precipitable material was markedly decreased and hardly any radioactivity was found in the neurohypophyseal proteins which were separated by polyacrylamide gel disc electrophoresis. As revealed by electron microscopy the SON cell bodies showed marked changes after treatment with colchicine: a deeply folded nucleolemma; a pronounced, granular nucleolus; a dispersed chromatin; a zonal distribution of cell organelles with mitochondria and lysosomes accumulated at the periphery, crowded ribosomes, often arranged as polyribosomes and richly branching short profiles of endoplasmic reticulum filled with filamentous material forming an inner perinuclear zone separated by enlarged Golgi complexes. The profiles of elongated Herring bodies in the infundibulum were increased. The axon terminals were filled with heavily osmiophilic neurosecretory granules. The neurofilaments were slightly or moderately increased in number. No apparent changes were observed with regard to the neurotubuli in the SON neurons. The glial cells of the supraopticoneurohypophyseal tract showed reactive changes with a proliferation of filamentous elements. The biochemical and ultrastructural findings are discussed especially with respect to the mechanisms of transport and release of neurosecretory granules.
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    Cell & tissue research 116 (1971), S. 564-577 
    ISSN: 1432-0878
    Keywords: Axons ; Dendrites ; Microtubules ; Endoplasmic Reticulum ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies of neural processes in the cerebellum, optic tectum, and cerebral hemisphere of the frog reveal a distinctive system of SER cisternae lying at intervals (commonly 1–2 μm apart) perpendicular to the long axis of axons and dendrites, interconnected by tubular, longitudinally orientated SER elements, and in direct continuity with the outer membrane of mitochondria. The transverse cisternae are fenestrated, with a single mierotubule (or rarely, two) passing through the centre of each 50–75 nm fenestration. Extensions of the SER-microtubule complex may be located parasynaptically in axon terminals and dendrites. The SER of dendritic spines also appears to be continuous with the fenestrated cisternae. Possible roles for the specialized SER (particularly of the parasynaptic extensions), such as calcium ion sequestration and ATP or monoamine oxidase transport, are discussed.
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  • 60
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    Keywords: Neurosecretion ; Actinomycin ; Protein synthesis ; Annelids (Oligochetes)
    Source: Springer Online Journal Archives 1860-2000
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    Description / Table of Contents: Zusammenfassung Die Wirkung von Aktinomycin auf die neurosekretorischen Q-und P-Zellen im Cerebralganglion von Enchytraeus wurde untersucht. Die Cytophotometrie lichtmikroskopischer Präparate von Q-Zellen ergab, daß in den ersten Stunden nach Aktinomycin-Behandlung eine deutliche Verminderung PAF-positiven Materials auftritt. Die ersten Veränderungen wurden elektronenmikroskopisch zwischen 1 und 4 Std nach Aktinomycin-Injektion beobachtet. Sie waren in beiden Zelltypen am eindeutigsten am Nucleolus. Es kommt zu einer Sonderung und räumlichen Trennung von granulärem und fibrillärem Material. Letzteres wird sehr stark vermehrt. In bezug auf Veränderungen der Strukturen des Cytoplasmas unterscheiden sich die Q-und P-Zellen besonders im Verhalten des Golgi-Apparates und der Ribosomen. Der Golgi-Apparat wird in den Q-Zellen kurze Zeit nach Applikation von Aktinomycin reduziert. In den P-Zellen persistiert er dagegen über alle beobachteten Zeitstufen hinweg. Die Ribosomen lösen sich von den Membranen in den Q-Zellen 4–8 Std nach Injektion, was in den P-Zellen nicht festzustellen ist. Diese Tatsachen führen zu der Annahme, daß das System der Proteinsynthese der P-Zellen relativ stabiler als das der Q-Zellen ist. Die in den späteren Zeitstufen beobachtete Normalisierung der Zellstrukturen läßt darauf schließen, daß die Wirkung des einmalig injizierten Aktinomycins 24 Std danach nachzulassen beginnt.
    Notes: Summary The influence of actinomycin on the neurosecretory Q and P cells of the brain of Enchytraeus was studied. Cytophotometrical measurements of Q cells in light mirocscopic preparations showed a significant decrease of PAF-positive material in the first hours after actinomycin application. At the ultrastructural level primary changes were established one to four hours after injection of actinomycin: In the nucleolus granular and fibrillar material became separated; there was a substantial increase of the fibrillar component. Concerning structural changes of the cytoplasm, Q and P cells differed especially with respect to the Golgi apparatus and the ribosomes. In the Q cells the Golgi apparatus had become greatly reduced shortly after actinomycin treatment. However, it persisted in P cells during all stages examined. Ribosomes became detached from membranes only in Q cells between 4 and 8 hours after injection. These data indicate that protein synthesis in P cells shows greater stability than in Q cells. The restitution of normal ultrastruoture during subsequent stages indicates that effects begin to subside 24 hours after a single injection.
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  • 61
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    Keywords: Hypothalamus (rat) ; Neurosecretion ; Air ions ; Diuresis
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    Description / Table of Contents: Résumé Les noyaux supra-optiques et paraventriculaires de l'hypothalamus du Rat albinos soumis aux ions atmosphériques positifs pendant 20 et 40 jours, présentent des signes d'hypo-activité: tendance à la diminution des volumes nucléaires et nucléolaires, augmentation des granules de neurosécrétat dans le cytoplasme des cellules neurosécrétrices, et diminution du matériel neurosécrétoire au long des axones. Corrélativement, la post-hypophyse est très appauvrie (surtout après 40 jours) en matériel coloré par l'hématoxyline chromique. On peut admettre, à la lumière de résultats antérieurs ou non publiés, que cette hypoactivité des neurones hypothalamiques est liée à une hyposécrétion d'hormone antidiurétique (A.D.H.). Cependant, on ne note pas de polyurie, mais une oligurie qui tend à disparaître une quinzaine de jours après le début du traitement; ceci suggère l'idée d'une réponse homéostatique hypothalamo-hypophysaire consécutive à un trouble du métabolisme hydrique induit par un autre facteur oligurique. Par ailleurs, Krueger a trouvé que l'aéro-ionisation positive entraine une augmentation du taux sanguin de sérotonine dont l'action antidiurétique au niveau rénal est bien connue. Ceci permet d'expliquer une composante oligurique initiale, provoquant en retour une inhibition hypothalamo-post-hypophysaire, de signification diurétique, cette réaction hypothalamique, ne normalisant la diurèse qu'au bout d'une quinzaine de jours, lors de l'épuisement de la réserve en ADH de la neuro-hypophyse. L'ensemble des modifications du métabolisme hydrominéral observées après aéro-ionisation positive peut s'expliquer de façon satisfaisante par l'augmentation du taux sanguin de sérotonine, contrebalancée progressivement par la réduction du taux d'ADH.
    Notes: Summary In the hypothalamus of albino rats submitted to positive air ions for 20 to 40 days, supra-optic and paraventricular nuclei exhibit various signs of hypo-activity: a slight decrease of nuclear and nucleolar volumes, an increase of the amount of neurosecretory granules in these neurones, while the migration of the neurosecretory material in the axons is diminished, almost no staining substance being detected along the axons. Simultaneously, the chrome-haematoxylin positive material is greatly reduced in the neurohypophysis, mainly after 40 days. Considering these data and other results, either unpublished or previously reported by various authors, it appears that the hypo-activity observed in the hypothalamo-neurohypophysial system may be correlated with a hyposecretion of antidiuretic hormone (ADH). There is no increase of diuresis; instead, an antidiuresis was noted which tends to disappear after about two weeks of treatment. This finding suggests that the hypothalamo-hypophysial response observed may be due to a disturbance of water metabolism induced by another antidiuretic factor. Besides, it was established that a treatment with positive air ions induces a significant increase of the serotonin level of the blood (Krueger). As an antidiuretic effect is directly exerted by serotonin on the kidneys, it may be responsible for the antidiuresis observed in this experiment. The inhibition of hypothalamic nuclei according to histological criteria would represent a homeostatic response trying to compensate the antidiuresis initially produced by serotonin, the neurohypophysial storage of ADH delaying the efficiency of this hypothalamic inhibition. All the available data on the modifications of water and electrolyte metabolism detected after a positive aero-ionization may be explained satisfactorily by an increase of the serotonin level of the blood, progressively counteracted by a decrease of ADH.
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    Cell & tissue research 107 (1970), S. 454-465 
    ISSN: 1432-0878
    Keywords: Cerebellum ; Neuron ; Synapses ; Myelin sheath ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In long-term organized cultures of newborn mouse cerebellum, granule cell neurons were studied with silver impregnation and electron microscopy. In silver impregnated cultures, small neurons are defined as granule cell neurons from their size, morphology and location. There are also occasional large nerve endings with the morphology typical of mossy fiber endings. In correlative electron micrographs, granule cell neurons revealed a faithful reproduction of characteristic structures seen in vivo. The fine structural details of cerebellar glomeruli and myelinated granule cell bodies developed in vitro were also described.
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    Cell & tissue research 107 (1970), S. 508-521 
    ISSN: 1432-0878
    Keywords: Synapses ; Axo-axonal ; Adrenergic and cholinergic terminals ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The relations between adrenergic and cholinergic terminals were studied in rat iris and rat heart with the electron microscope. Adrenergic terminals were identified by treating the animals with 5-hydroxydopamine, which produces dense-cored synaptic vesicles in adrenergic terminals in tissues fixed in glutaraldehyde and osmium. The specificity of this observation was verified. It was found that adrenergic and cholinergic nerve terminals often come in close contact with one another, the distance between the adjoining membranes being about 250 Å. At times, faint membrane thickenings could be observed in these places. The available pharmacological, physiological, and morphological evidence leaves little room for doubt that cholinergic terminal fibres can influence the adrenergic fibres. From mainly morphological evidence, it is also postulated that adrenergic terminals influence cholinergic ones.
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    Cell & tissue research 111 (1970), S. 316-345 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Histophysiology of median eminence ; Avian neurohypophysis ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
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    Description / Table of Contents: Résumé Les effets de l'adénohypophysectomie et de diverses sollicitations de l'axe hypothalamo-hypophysio-corticosurrénalien sur l'ultrastructure de l'Eminence Médiane (E.M.) ont été étudiés chez le Pigeon. 1. Chez le Pigeon entier, l'Eminence Médiane Caudale (E.M.C.) se distingue de l'Eminence Médiane Rostrale (E.M.R.) essentiellement par l'absence dans les deux couches les plus externes (couches palissadique et superficielle) de l'E.M.C. de granules de gros calibres (1600 à 1900 Å), la rareté de granules de diamètre moyen (1200–1400 Å) et la prédominance de petites vésicules à cœur dense de 600–800 Å. 2. La préhypophysectomie entraine: a) dans l'E.M.R. la quasi disparition de granulations dans les deux couches externes; b) dans l'E.M.C. la ≪vidange≫ de nombreux axones, mais un enrichissement relatif, parmi les granulations restantes, des granulations de gros calibre (1600–1900 Å) aux dépens des granules de plus petit calibre. 3. Un shock insulinique entraine des modifications du même ordre: a) déplétion des granules denses, limitée dans ce cas à la portion la plus antérieure des deux couches externes de l'E.M.R.; b) enrichissement relatif des granulations de moyen (1200–1400 Å) et de gros (1600–1900 Å) calibre dans l'E.M.C. avec, en plus dans l'E.M.C., un enrichissement en vésicules de type synaptique. 4. Un traitement à la métopirone produit un accroissement du nombre des granulations de moyen (1200–1400 Å) calibre dans les couches externes de l'E.M.R. et de l'E.M.C., et un enrichissement important de l'E.M.C. en vésicules de type synaptique. 5. Le traitement à la prednisolone conduit à un enrichissement très marqué des couches externes de l'E.M.R. en grains de 1200–1400 Å, et à un enrichissement des couches externes de l'E.M.C. en granulations de 1000 Å. Ces résultats sont discutés dans la perspective des régulations hypothalamo-corticotropes, particulièrement en ce qui concerne les granules de 1200–1400 Å.
    Notes: Summary The effects of adenohypophysectomy, and of several experimental interventions on the hypothalamo-pituitary-adrenal cortical axis have been studied in relation to the fine structure of the median eminence in the pigeon. 1. In control animals, the following morphological features of the caudal median eminence (C.M.E.) distinguish it from the rostral median eminence (R.M.E.): a) the absence in both external layers of the C.M.E. of large (1,600–1,900 Å) electron-dense granules, b) the presence in the C.M.E. of a small number of medium-size (1,200–1,400 Å) granules, and c) the predominance in the C.M.E. of small (600–800 Å) dense-core vesicles. 2. Adenohypophysectomy leads to: a) almost complete disappearance of electron-dense granules in both external layers of the R.M.E., and b) “emptying” of numerous axons and a relative increase in the number of large (1,600–1,900 Å) granules in the C.M.E. 3. Insulin shock produces modifications similar to those of adenohypophysectomy. The depletion of electron-dense granules from the axons is, however, restricted to the most anterior part of the R.M.E., and, in the C.M.E., the relative increase in the number of larger granules affects the 1,200–1,400 Å and the 1,600–1,900 Å size granules. 4. Metopirone enhances the number of medium-size (1,200–1,400 Å) granules in the external layers of both the R.M.E. and the C.M.E. and causes a significant increase in the number of synaptic-like vesicles in the C.M.E. 5. Prednisolone treatment leads to a marked enrichment of the external layers of the R.M.E. with 1,200–1,400 Å granules, and of the external layers of the C.M.E. with 1,000 Å granules. These results have been discussed with special reference to the hypothalamic control of the adrenocorticotropic function, especially reviewing the role of the 1,200–1,400 Å granules.
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    Cell & tissue research 111 (1970), S. 572-585 
    ISSN: 1432-0878
    Keywords: Brain ; Intraventricular cellular processes ; Ependyma ; Lateral ventricle ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ependym und subependymale Strukturen der Seitenventrikel von Katzen aus dem Bereich des Nucleus caudatus und des Corpus callosum wurden nach Perfusionsfixierung elektronenmikroskopisch untersucht. Dort findet sich eine Reihe von Neuritenanschnitten, deren kolbenförmige Auftreibungen leere Vesikel, “dense core granula” und Mitochondrien enthalten. Neben diesen neuronalen Elementen werden auch Zellfortsätze nichtneuronaler Natur im Ventrikellumen beobachtet, die Ependym- und intraventrikulären Zellen entstammen. An umschriebenen Stellen nehmen diese kolbig aufgetriebenen Fortsätze desmosomenartige Kontakte mit dem Ependym auf. An diesen Orten umgeben Mikrovilli die Fortsätze korbartig. Ob es sich bei diesen Kontakten um synapsenartige Strukturen handelt, oder um „Rezeptoren“ für unbekannte chemische Stoffe, wird diskutiert. Die Kontakte sind unabhängig von der Form der Ependymzellen und davon, ob diesen graue oder weiße Substanz unterliegt. Zwischen und in den Ependymzellen finden sich — bedingt durch den unterschiedlichen Gehalt an Zellorganellen — drei verschiedene Arten von Fortsätzen. Die Zahl der neuronalen Fortsätze in diesem Bereich ist sehr viel kleiner als im Ventrikellumen.
    Notes: Summary Ependyma and subependymal regions of the lateral ventricles of cats (area of Nucleus caudatus and Corpus callosum) were fixed by perfusion and investigated electronmicroscopically. Intraventricular axons showing a beaded shape with smaller and thicker parts were found. The varicosities contain empty vesicles, dense core granules and mitochondria. Beside those certainly neuronal elements there exist intraventricular cell processes originating from ependymal cells and free cells. The neuronal processes make desmosome-like contacts with the ependyma. These regions of contact are surrounded by basket-like arranged microvilli. It is discussed, whether the contacts function like synapses or as receptors for a substance, which is still unknown. Contacts do not depend on the shape of ependymal cells. They exist with the ependyma of Nucleus caudatus (grey substance) as well as with the ependyma of Corpus callosum (white substance). Because of the different contents of cell-organelles one can differentiate three different sorts of processes situated inter- or intracellularly in the ependyma. Neuronal processes within the lateral ventricles are more numerous than those found within the ependymal layer.
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    Cell & tissue research 111 (1970), S. 179-194 
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Rat cerebral cortex ; Synapses ; Postnatal development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptosomes (nerve-ending particles) prepared from the cerebral cortex of rats, aged 2–21 days, were examined after fixation in glutaraldehyde and osmium tetroxide, in order to study the development of the contact region between their pre- and postsynaptic components. Synaptosomes were present at all ages studied, although they increased in number and underwent morphological changes, e.g. a decrease in ribosomes and increase in synaptic vesicles, during development. The seventh postnatal day appeared to be a critical period for development, as many adult features were first observed at this time. The synaptic contact region was characterized by desmosome-like symmetrical thickenings until the fourth postnatal day, after which it became increasingly asymmetrical. The postsynaptic thickening, which had been undifferentiated until the seventh day, underwent a focalization of its material at this stage to form postsynaptic densities typical of the adult synapse. Cleft material was present at all ages, although for the first few days it was sparse and comparatively unorganized. An attempt has been made to distinguish between synaptosomes derived from axosomatic and axodendritic endings. The possible significance of the early desmosome-like thickenings is discussed, and the role of the cleft material and post-synaptic densities in the development of the synapse is analysed.
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    Cell & tissue research 111 (1970), S. 293-315 
    ISSN: 1432-0878
    Keywords: Herring bodies ; Ultrastructure ; Degeneration ; Regeneration ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Herring bodies in the posterior lobe of the bovine hypophysis are very large (2–600 μ) and can be classified into three types. The type I Herring body contains an accumulation of neurosecretory granules. These Herring bodies are very scarce and should not be confused with the numerous, but small, axonal swellings which also contain neurosecretory granules. The type II Herring body is characterized by the presence of a varying number of normal, moderately electron dense and “empty” vesicles, autophagic vacuoles, multilamellate bodies and occasional mitochondria. These Herring bodies are frequently observed. The type III Herring body is typified by the presence of dense vesicles connected to tubular formations which contain material of variable electron density, of filaments, and of long slender and very numerous mitochondria. The presence of multilamellate bodies and autophagic vacuoles suggests that the type II Herring body is in a degenerating phase. This concept is further substantiated by the similarity between this type of Herring body and transected neurosecretory axons in which degeneration is occurring. A similar comparison suggests that the type III Herring body is undergoing a regenerative process. Our current concept of the structure and function of Herring bodies is revised in the discussion.
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    Cell & tissue research 109 (1970), S. 573-582 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Herring Bodies ; Lysosomal Residues ; Aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Anschluß an frühere Beobachtungen über die Reaktion von Herringk: orpern auf osmotische Belastung wurden die Veränderungen dieser Strukturen mit zunehmendem Alter studiert. In parallelen färberisch-lichtmikroskopischen und elektronenmikroskopischen Untersuchungen wurden die im Gebiet der Nuclei supraopticus und paraventricularis gelegenen Herringkörper von jungen (3 Monate) und alten (15–20 Monate) Ratten miteinander verglichen. Bei alten Tieren findet man im Lichtmikroskop eine starke Zunahme der durch Neurosekretfärbung darstellbaren Herringkörper und im Elektronenmikroskop eine Füllung dieser Gebilde mit „dense bodies“ und Myelinkörpern. Diese werden als lysosomale Residualkörper gedeutet, die aus den neurosekretorischen Perikarya in die Zellfortsätze abgeschoben werden und sich dort mit zunehmendem Alter anhäufen. Die fraglichen Zellfortsätze werden aufgrund von morphologischen und topographischen Gesichtspunkten als Dendriten angesehen.
    Notes: Summary In continuation of previous investigations on the reaction of neurosecretory Herring bodies to osmotic stress, the alterations of these structures with increasing age were studied. The Herring bodies located in the vicinity of the supraoptic and paraventricular nucleus of young (3 months) and aged (15–20 months) rats were compared by means of light and electron microscopy. In old rats, the light microscope reveals a strong increase in the number of Herring bodies as visualized by neurosecretory stain whereas the electron microscope exhibits an accumulation of dense bodies and myelinated bodies inside these structures. These are interpreted as lysosomal residual bodies which are transported from the neurosecretory pericarya into the cell processes, thus accumulating with increasing age. Morphological and topographical considerations lead to the assumption that the storage sites are dilatations of dendrites.
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    Cell & tissue research 110 (1970), S. 540-558 
    ISSN: 1432-0878
    Keywords: Cerebral cortex ; Synapses ; Rat ; Postnatal differentiation ; Morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of layers I and II of the motor cerebral cortex of rat brain has been studied at birth, 4, 7 and 14 days postnatal and in the adult. Compared with the adult, neonatal rat motor cortex exhibited a large extracellular space which decreases with increasing age. At all stages studied the neurons were seen to contain the organelles usually found in adult neurons. Growth cones were present in decreasing numbers up to 14 days old. Synapses were detectable at birth and there was an obvious increase in their number throughout the postnatal development. At the earliest stages studied there was a lack of specialization characteristic of the adult. Many synapses were either avesicular or relatively so and lacked the high degree of modification of adult pre- and postsynaptic membranes. By 7 days after birth many synapses existed which in all morphological respects resembled those of the adult, and by 14 days, the majority were of the adult type. These findings, particularly with reference to the postnatal development of synapses, have been discussed in relation to the known electrophysiological findings.
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    Cell & tissue research 108 (1970), S. 190-224 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohaemal areas ; Invertebrates ; Lymnaea stagnalis L. ; Cytology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the central ganglia of Lymnaea stagnalis neurosecretory cell groups have previously been identified by means of chrome-haematoxylin or paraldehyde-fuchsin stains. In the present study seven types have been distinguished within the class of Gomori-positive cells on the basis of different staining reactions with the alcian blue/alcian yellow technique. Five types are located in the cerebral ganglia and in the lateral lobes, whereas two cell types occur in the ganglia of the visceral ring. No neurosecretory cells have been observed in the buccal and pedal ganglia. The staining technique used proved to be superior to the classic neurosecretory stains, because with this method the secretory substances can easily be distinguished from nonsecretory Gomori-positive tissue constituents. One of the two Gomori-negative neurosecretory cell types of the cerebral ganglia react positively with the alcian blue/alcian yellow technique. In addition, two Gomori-negative neurosecretory cell types, which had not been described before, were identified in the visceral ring. The ultrastructure of the four neurosecretory cell types in the visceral ring is described. The electron microscope revealed that each of the histochemically distinguished secretory substances consists of elementary granules which differ in size and appearance from each other and from the neurosecretory elementary granules which have been described by other authors in the cerebral ganglia and in the lateral lobes. The neurohaemal areas of the neurosecretory cells in the visceral ring are very extensive and include the peripheral parts of the nuchal nerves and of the connectives and nerves of the ganglia of the ring. The perineurium and the adjacent parts of the connective tissue which surround the ganglia, the connectives and the nerves are regarded as additional neurohaemal zones, because in these regions many tiny nerves occur, which consist mainly of neurosecretory axons ending non-synaptically near parts of the vascular system. In the perineurium surrounding the cerebral ganglia and their neurohaemal area a similar network of neurosecretory fibres was observed. Indications of release of the secretory material were regularly observed. Release apparently takes place by exocytosis. A circadian rhythmicity was observed in the release activity of some of the neurosecretory cell types.
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    Cell & tissue research 108 (1970), S. 282-296 
    ISSN: 1432-0878
    Keywords: Synapses ; Complex ; Nuclei vestibulares ; Lampetra planeri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The vestibular fibres of ammocoetes of Lampetra planeri form a peculiar type of synaptic contact with the large nerve cells of the vestibular nuclei. In fact, a large expansion of the fibre is enveloped by the nerve cell cytoplasm, which makes a sort of spoon or actual channel by sealing its own plasma membrane with adhesion plaques around the fibre. In the whole area of juxtapposition between the fibre and the cell membranes, three different types of specialized contacts occur: desmosomoid junctions, which are the most numerous; zones having the characteristics of chemically active synapses on account of the presence of vesicles clustered on the presynaptic side; gap-junctions, where electrical transmission might occur. A puzzling feature is the presence of an enormous number of presynaptic vesicles located in the central part of the axoplasm of the fibre expansion.
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    Cell & tissue research 108 (1970), S. 563-581 
    ISSN: 1432-0878
    Keywords: Insect ; Brain ; Deutocerebrum ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Feinstruktur des Glomerulineuropils im Antennenhügel (sensorisches Zentrum) der Wanderheuschrecke Locusta migratoria wird beschrieben. Die Glomeruli stellen ein kompliziertes Netzwerk von sensorischen Antennennervenfasern und interneuronalen hirneigenen Elementen dar. Die Nervenfasern sind in ihrer großen Mehrheit von klaren synaptischen Vesikeln gefüllt, die sich mit Zinkjodid-Osmiumsäure färben (Akert und Sandri, 1968). Zwei Synapsentypen treten auf, die sich vor allem durch die Form und Ausdehnung des Synapsenspaltes unterscheiden. Beide Typen zeigen eine morphologische Polarität. Paramembranöses synaptisches Material läßt sich besonders deutlich mit der Wismutjodid-Methode von Pfenninger et al. (1969) darstellen. Diese Methode, die den gleichen Effekt wie bei Wirbeltieren zeigt, scheint zur Beurteilung synaptischer Verhältnisse (Verteilung, Häufigkeit) besonders geeignet. Normalerweise sind über synaptische Kontakte drei aneinandergrenzende Nervenfasern miteinander verknüpft. Obwohl Degenerationsexperimente vorgenommen wurden, konnte die synaptische Verschaltung der verschiedenen Fasern nur teilweise geklärt werden. Sensorische Fasern sind mit interneuronalen Fortsätzen verbunden, die prä- und postsynaptische Zonen haben. Es gibt einige Hinweise, daß auch sensorische Fasern miteinander synaptisch verknüpft sind. Vesikelfreie postsynaptische Elemente sind sehr selten, während eine synaptische Verbindung über „axo-axonale“ Kontakte häufig vorkommt. Die Antennalglomeruli mit ihrer großen Anzahl von kleinen, mit synaptischen Vesikeln gefüllten Fasern sind deutlich anders organisiert als die Glomeruli im Pilzkörperkelch (vgl. Lamparter et al., 1969).
    Notes: Summary The fibre anatomy of the glomeruli neuropil of the antennal lobes (sensory centres) of Locusta migratoria has been analysed by electron microscopy. The glomeruli consist of interneuronal and sensory elements of the antennal nerve, forming a complicated mesh-work of fine fibres. The great majority of nervous fibres is filled with clear synaptic vesicles, which stain with zinc-iodide-osmic-acid (Akert and Sandri, 1968), Two types of synaptic junctions are found, differing especially in shape and size of their synaptic gap. Both types show a clear morphological polarity, so that the distinction of pre- and postsynaptic fibres is possible. Inter- and intracellular paramembraneous synaptic material can be demonstrated by the bismuth iodide impregnation of Pfenninger et al. (1969) This method, showing the same effect as in vertebrate nervous systems, seems to be very adequate for the identification of synaptic structures and synaptic distribution. Normally presynaptic structures are situated in angles of nerve fibres. Although degeneration experiments were made, the synaptic interaction of the various fibre types could only partially be explained. Sensory fibres interact with intemeurons, which show pre- and postsynaptic zones. They seem to be responsible for the integration of different glomeruli. There is some evidence that sensory fibres have synaptic connections, too. Postsynaptic profiles with several synaptic contacts and free of vesicles are very rare, whereas “axo-axonic” contacts are found quite often. These glomeruli with their great number of vesicle-filled profiles differ from corpora pedunculata calyx glomeruli (see Lamparter et al. 1969).
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    Cell & tissue research 109 (1970), S. 64-82 
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    Keywords: Median eminence ; Avian neurohypophysis ; Neurosecretion ; Electronmicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'étude ultra-structurale de l'éminence médiane (E.M.) du Canard, réalisée à l'aide d'une fixation par perfusion intracarotidienne fait apparaître plusieurs traits saillants particulièrement remarquables: a) Dans la couche sous-épendymaire, l'existence de neurones infundibulaires dispersés, de fibres parfois dilatées renfermant des granulations à cœur dense, et de vaisseaux structuralement aptes à drainer des sécrétions. b) Dans la couche fibreuse, la présence d'axones appartenant au faisceau hypothalamopost-hypophysaire, dont les granulations semblent se différencier comme dans la Pars Nervosa. c) Dans la couche palissadique l'existence de trois types de fibres neurosécrétoires au moins: Type 1: Granulations comprises entre 1100 et 1600 Å (avec une subdivision possible: 1100–1300 et 1300–1600 Å), associées éventuellement à des vésicules de 400–600 Å. Type 2: Granulations ≦1000 Å (pouvant être également subdivisées en deux catégories: 800 Å et 1000 Å), avec généralement de nombreuses vésicules de 400 à 600 Å. Type 3: Vésicules de 400 à 600 Å, sans granulations. La différence entre les régions rostrale et caudale de l'E.M. est due essentiellement à une variation dans les proportions relatives de ces différentes catégories axonales, le type 1 étant plus abondant dans la région antérieure, et le type 2 dans la postérieure.
    Notes: Summary Ultra-structural studies, performed after fixation by intracarotidal perfusion, have revealed some peculiar features in the duck median eminence (M.E.): a) Within the subependymal layer, there were scattered neurons belonging to the nucleus infundibularis, axons and varicosities with dense core granules, and capillaries with structural characteristics similar to those in the superficial layer; b) Within the fiber layer, were seen the axons of the hypothalamo-neurohypophysial tract, with granules showing the same differentiation as found within the pars nervosa; c) Within the palisade layer, there was evidence for at least three main types of neurosecretory axons: Type 1 axons, containing granules ranging from 1,100 to 1,600 Å (with a possible subdivision into 1,100–1,300 Å and 1,300–1,600 Å), possibly associated with 400–600 Å vesicles. Type 2 axons, including granules ≦1,000 Å (with a possible subdivision into 800 and 1,000 Å), associated with numerous 400–600 Å vesicles. Type 3 axons, containing 400–600 Å vesicles, but without dense granules. At the ultrastructural level, the main difference between the rostral and caudal M.E. essentially lies in the relative percentage of these 3 axon types. Type 1 axons were found to be more numerous within the anterior M.E., whereas Type 2 axons were more abundant in the caudal region.
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    Cell & tissue research 110 (1970), S. 336-349 
    ISSN: 1432-0878
    Keywords: Nervous system ; Flies ; Intermediate retina (Lamina) ; Centrifugal fiber system ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By combining the Golgi and the electronmicroscope techniques it has been possible to identify accurately the system of centrifugal fibers which arborizes in the lamina of muscoid flies forming the so-called nervous bags. Each of them originates from a single fiber entering the lamina at the site in which the second order and the long visual fibers leave it. This single fiber represents the peripheral portion of a T-shaped trunk stemming from a small neuronal body located in the external region of the medulla. The central branch terminates within the first synaptic field of this visual center. After entering the lamina the centrifugal fiber ramifies profusely and its branches can be seen climbing and synapsing on the surface of the photoreceptor axon endings. The synaptic loci show characteristic synaptic ribbons located within the nervous bag fibers. This fact suggests that direction of conduction is from the medulla to the lamina. This study has also revealed that the intramedullar terminals of the centrifugal fibers establish intimate contacts with one of the two second order fiber endings.
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    Keywords: Neurosecretion ; Gastropoda ; Fluorescence ; Electron microscopy
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    Description / Table of Contents: Zusammenfassung Durch Vergleich fluoreszenz- und elektronenmikroskopischer Untersuchungen am zentralen Nervensystem von Planorbarius corneus L. wird nachgewiesen, daß in den Schlundringganglien Neurosekretzellen vorkommen (Nachweis mit Pseudoisocyaninchlorid), die mit Nervenzellen nicht identisch sind, die durch ihren hohen Gehalt an biogenen Aminen auffallen (Nachweis durch die Methode von Falck und Owman, 1965). Es können daher im Schlundring von Planorbarius corneus peptiderge und aminerge Neurosekretzellen unterschieden werden. Die PSC-positiven Neurosekretzellen enthalten elektronendichte Elementargrana und die aminergen Neurone „dense-core“ Vesikel. Der Nachweis biogener Amine in einigen Nervenzellen von Planorbarius corneus spicht für deren chemische Identität mit Transmittersubstanzen, ihre hohe Konzentration aber für eine Abgabe in die Körperflüssigkeit.
    Notes: Summary The neurosecretory system of the snail Planorbarius corneus has been investigated by fluorescence and electron microscopy. With the fluorochrome Pseudoisocyanin the established neurosecretory system in the cerebral ganglia and single neurosecretory cells in the other ganglia show an intensive yellow fluorescence. Electron micrographs reveal the presence of electron dense granules (elementary granules) in the pericarya and the axons of neurones which have the same localisation in the ganglia as the pseudoisocyanin-positive cells. The fluorescence technique for biogenic amines produces yellow and green fluorescence within neurons and in the neuropil and nerves. The fluorescence obtained in determinable areas and neurones correlates well with the electron microscopic location of “dense-core” vesicles within the pericarya and axons of cells in even the same areas. It is discussed, that in this animal both types of cells are so-called “neurosecretory cells”, because the high content of elementary granules in the “peptidergic neurosecretory cells” and of “dense-core” vesicles in the “aminergic neurosecretory cells” is an indication for secretion of these products in neurohaemal areas (circulatory channels).
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    Cell & tissue research 110 (1970), S. 559-568 
    ISSN: 1432-0878
    Keywords: Cerebral cortex ; Rat ; Synapses ; Postnatal ; Quantitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Quantitation of synapses at different postnatal ages has been undertaken in the cerebral cortex of the rat. In this study axial ratios of presynaptic bags, proportion of cortex occupied by presynaptic bags and numbers of synapses per unit volume of cortex have been estimated. Observations on synaptic vesicle packing densities have also been made. Synaptic bags become increasingly spherical up to 7 days of age and become more elongated thereafter. The proportion of cortex occupied by presynaptic bags increases rapidly up to 7 days of age and then at a decelerated rate up to maturity. The number of synapses per unit volume increases slowly over the first four days after which there is a rapid increase to 14 days, followed by a decelerated rate. The average presynaptic bag shows marked changes in volume with increasing age which indicate the probability of two stages of synaptic development. This two stage development is further reflected in the estimates on vesicle packing densities. The implications of the results are discussed in relationship to changes in functional activity of the cortex during postnatal development.
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    Cell & tissue research 111 (1970), S. 195-208 
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Rat cerebral cortex ; Glutaraldehyde/PTA ; Synapses ; Postnatal development
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Synaptosomes derived from 2–21 days postnatal rat cerebral cortex have been examined following glutaraldehyde fixation and block PTA staining, with the aim of investigating the maturation of the paramembranous densities at the contact region between the pre- and postsynaptic components. The internal coats of pre- and postsynaptic membranes first appear as undifferentiated plaque-like thickenings, which gradually develop into, or are replaced by, dense projections and postsynaptic focal densities respectively. Both sets of densities pass through an interconnected phase before starting to emerge as discrete entities at 5–7 days. The external coats of the pre- and postsynaptic membranes coalesce to form a plate-like structure which breaks down during development to form the cleft densities or transverse bars of the adult contact region. Although for the first few days of postnatal development only one type of synaptosome can be identified, from 5 days onwards two types corresponding to types A and B of adult life become recognizable. Increase in height of the dense projections has been correlated with increase in the number of synaptic vesicles per synaptosome during postnatal development, indicating that the synaptic vesicles may play a role in the formation and maturation of dense projections. The possible importance of other factors in this process is also discussed.
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    Cell & tissue research 105 (1970), S. 137-154 
    ISSN: 1432-0878
    Keywords: Echinoderms ; Nervous System ; Radial Nerve (Lange) ; Asteriasrubens ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Langes Nerv, ein Teil des hyponeuralen Nervensystems der Asteroiden, wurde licht-, fluoreszenz- und elektronenmikroskopisch untersucht. Er zeigt die typische Gliederung in Kern- und Faserschicht. Die großen unipolaren Ganglienzellen reichen mit kolbenförmigen Ausläufern in das Cölom. Ihre basalen Fortsätze ziehen in die Faserschicht. Cölomepithelzellen liegen den Nervenzellen in unregelmäßigen Abständen als Deckzellen kappenartig auf oder sind als Stützzellen ausgebildet. Deck- und Stützzellen werden als Gliazellen aufgefaßt. Unterhalb der Kernschicht wurden licht- und elektronenmikroskopisch Zellen beobachtet, die für neurosekretorische Elemente gehalten werden. Der Feinbau der erwähnten Zelltypen wird beschrieben und diskutiert. Der histochemische Nachweis auf biogene Amine in Langes Nerv fiel negativ aus.
    Notes: Summary The nerve of Lange, part of the starfish hyponeural nervous system, was investigated with the light-, fluorescence- and electron microscopes in Asterias rubens. It consists of two layers, one containing perikarya, the other fibres. The big nerve cells are unipolar and possess club-shaped processes which extend into the coelom. At their basis, the axon merges into the fibrous layer. Irregularly spaced coelomic epithelial cells cover partly the ganglion cells or constitute supporting elements. Covering and supporting cells are interpreted to represent glia cells. Underneath the perikarya layer, cells have been found by both the light and electron microscopes which are considered to be neurosecretory elements. The fine structure of the various cell types is described and discussed. Histochemical tests for biogenic amines were negative.
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    Cell & tissue research 103 (1970), S. 483-501 
    ISSN: 1432-0878
    Keywords: Cerebrospinal fluid ; Contacting neurons ; Paraventricular nucleus ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Der neurosekretorische Nucleus paraventricularis von Reptilien wurde licht- und elektronenmikroskopisch untersucht. Zellen dieses Kernes bilden ventrikuläre Fortsätze, welche mit knöpfchenförmigen Endigungen — „Liquorkontaktnervenendigungen“ — in das Lumen des 3. Ventrikels vordringen. — Der Nucleus paraventricularis der Sumpfschildkröte (Emys orbicularis) besteht aus einer proximalen, am Ependym liegenden Zelllage und einer distalen Nervenzellgruppe. Zwischen den Zellreihen sind synaptische Zonen vorhanden. Elektronenmikroskopisch weisen die knöpfchenförmigen, mit dem Liquor in Kontakt stehenden Nervenendigungen Elemente des endoplasmatischen Retikulums, freie Ribosomen und eine wechselnde Anzahl von Mitochondrien auf. Die Endigungen enthalten neurosekretorische Elementargranula (Durchmesser 1300–1800 Å) und sind mit atypischen Zilien versehen, zu denen meist zwei Basalkörperchen mit langen Zilienwurzeln gehören. Die Liquorkontakt-Endigungen sind mit den benachbarten Ependymzellen und mitunter untereinander durch Desmosomen verbunden. — Im Lumen des 3. Ventrikels treten auch dünne, Katecholamingranula enthaltende Axone auf, die mit den den Liquor erreichenden Nervenendigungen des Nucleus paraventricularis Synapsen bilden. Synapsen werden außerdem auf den ventrikulären und peripheren Fortsätzen und an den neurosekretorischen Perikaryen beschrieben. In den synaptischen Zonen findet man axodendritische Synapsen, deren Dendrit neurosekretorische Elementargranula enthält. — Die morphologische Ähnlichkeit zwischen neurosekretorischen Liquorkontakt-Nervenendigungen und denen des Liquorkontakt-Neuronensystems wird hervorgehoben. An Hand der Ergebnisse wird die Bedeutung und Funktion der neurosekretorischen, mit dem Liquor in Kontakt tretenden Nervenendigungen im Rahmen des Informationsaustausches zwischen Liquor cerebrospinalis und dem neuroendokrinen System diskutiert.
    Notes: Summary The neurosecretory paraventricular nucleus of reptilia was studied light and electron microscopically. Nerve cells of this nucleus form ventricular processes which as club-like endings — “liquor contacting nerve endings” — protrude into the lumen of the 3rd ventricle. — The paraventricular nucleus of the turtle, Emys orbicularis, is built up of a proximal neuronal layer situated close to the ependyma, and of a distal group of neurons. Synaptic zones can be found between the cell rows. As demonstrated electronmicroscopically, the club-shaped liquor contacting nerve endings show elements of the endoplasmic reticulum, free ribosomes and mitochondria in various amounts. The terminals contain neurosecretory elementary granules (diameter about 1,300–1,800 Å) and bear atypical cilia supplied mostly with two basal bodies and long rootlet fibres. The liquor contacting nerve endings are connected by desmosomes with the neighbouring ependymal cells and sometimes with one another. — In the lumen of the 3rd ventricle, also small axons containing catecholamine granules, can be found. These neurites form synapses with the liquor contacting nerve terminals of the paraventricular nucleus. Furthermore, synapses situated on the ventricular and peripheral nerve processes and on the neurosecretory perikarya, are described. In the synaptic zones, axodendritic synapses occur; the dendrite contains neurosecretory elementary granules. — The morphological similarity between neurosecretory liquor contacting nerve terminals and those of the liquor contacting neuronal system is stressed. On the basis of the results, the significance and function of the neurosecretory liquor contacting terminals is discussed in connection with the exchange of informations between cerebrospinal fluid and the neuroendocrine system.
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    Cell & tissue research 103 (1970), S. 544-558 
    ISSN: 1432-0878
    Keywords: Supraoptico-hypophysial system ; Rabbit ; Neurosecretion ; Nucleus paraventricularis ; Nucleus supraopticus ; Nucleus suprachiasmaticus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Zwischenhirn von 16 Kaninchen wurde das supraopticohypophysäre System mit Neurosekretfärbungen untersucht. Die Ausdehnung der großzelligen Kerngebiete und der Verlauf ihrer Axone wurde den Ergebnissen von Ford und Kantounis (1957) gegenübergestellt. Die Ganglienzellen des Nucleus paraventricularis lassen sich morphologisch und topographisch in zwei Gruppen unterteilen. In den Ganglienzellen des Nucleus suprachiasmaticus kommen Granula vor, die mit Paraldehydfuchsin, Gallocyanin und Pseudoisocyanin dargestellt werden können. Im Infundibulum der untersuchten Kaninchen findet sich eine Doppelung des Tractus supraoptico-hypophyseos. Paraldehydfuchsin-, gallocyanin- und pseudoisocyaninpositives Material in der Adenohypophyse und solches im Zwischenlappen wird beschrieben; die Substanz im Zwischenlappen kann als Neurosekret gedeutet werden.
    Notes: Summary In the diencephalon of 16 rabbits the supraoptico-hyophysial system was studied by means of special stainings for neurosecretory substances. The extent of the magnocellular nuclear areas and the course of the respective axons were traced and compared with the findings of Ford and Kantounis (1957). Morphologically and topographically the nerve cells of the nucleus paraventricularis can be subdivided into two groups. In the nerve-cell bodies of the nucleus suprachiasmaticus neurosecretory granules are demonstrated by means of paraldehydfuchsine, gallocyanine and pseudoisocyanine. In the infundibulum of the rabbits investigated there is a duplication of the supraoptico-hypophysial tract. Substances reacting with paraldehydfuchsine, gallocyanine and pseudoisocyanine are found in both the anterior and intermediate lobes of the hypophysis; the substance found in the intermediate lobe is regarded as a neurosecretory material.
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    Cell & tissue research 103 (1970), S. 559-569 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Guinea-pig ; Fluorescence ; Neurosecretion
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    Notes: Summary Sections of guinea-pig hypothalamus stained by various methods have been studied under bright-field and fluorescence microscopy to determine the characteristics of refractile granules originally observed in fixed tissue stained for cholinesterases but also seen in unfixed, unstained material. When sections of fresh tissue or tissue fixed in formaldehyde: Na2SO4 were examined by fluorescence microscopy, the granules emitted with a wave-length between 600 and 620 mμ. The density and distribution of the granules closely paralleled that of one of the components that stained with the aldehyde-fuchsin and chrome-alum techniques for neurosecretory substance; granules were absent, however, from the area characterized by Herring substance, and from the stained perikarya and beaded axons of the supraoptic and paraventricular nuclei. The granules were most numerous in the median eminence and infundibular nuclei. Their concentration was about “average” in mature males and in lactating and hypophysectomized females; they were more abundant in late pregnancy and after ovariectomy and were particularly plentiful some weeks after hysterectomy. They were virtually absent from immature males and females. The possible relation of the granules to releasing factors or to vasotocin-like neurosecretory material is discussed.
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    Cell & tissue research 104 (1970), S. 107-115 
    ISSN: 1432-0878
    Keywords: Cephalopoda ; Eledone ; Neurosecretion ; Ultrastructure ; Cardioaccelerator
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary 1. It is thought that the extensive system of nerves passing into the wall of the anterior vena cava in Eledone cirrosa has a neurosecretory function. Examination of the fine structure of the nerve terminations demonstrated the presence of large numbers of electron-dense granules similar to those described in known neurosecretory tissue. 2. Extracts of the anterior vena cava have a persistent excitatory effect on the isolated Eledone heart. The activity is not attributable to 5-hydroxytryptamine, noradrenaline or adrenaline. The active factor is heat stable. The greatest level of activity was found in the inner layers of the vessel in the region where the nerves pass into the wall of the vessel, i.e. the distribution of the active principle parallels that of the nerve terminations in the vein. 3. The physiological role of the system is discussed.
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    Cell & tissue research 104 (1970), S. 517-529 
    ISSN: 1432-0878
    Keywords: Hypothalamo-hypophysial system ; Neurosecretion ; Guinea pig ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The time and place of occurrence of the neurosecretory substance in the hypothalamo-neurohypophysial system of the guinea pig during embryogenesis have been investigated. Use is made of the luminiscence of neurosecretion stained with paraldehydefuchsin when observed in a dark field. It is established that the neurosecretory material occurs first in some cells of the supraoptic nucleus about the 39th–40th day of intrauterine development. In the paraventricular nucleus it is observed about the 44th–45th day. At that time it is seen also in Eminentia mediana and in the neurohypophysis. In the latter, however, it is in a smaller amount than in the areas situated above it. These results are discussed in connection with the transport theory of Bargmann and Scharrer.
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    Keywords: Hypothalamus (Rat) ; Neurosecretion ; Air ions ; Adrenals ; Diuresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les noyaux supraoptiques et paraventriculaires de l'hypothalamus du Rat albinos soumis aux ions atmosphériques négatifs pendant 3 à 5 semaines, présentent des signes d'hyperactivité: augmentation significative des volumes nucléaires et nucléolaires, diminution des granules de neurosecrétat dans le cytoplasme des cellules neurosecrétrices et augmentation du matériel neurosecrétoire au long des axones. Corrélativement la post-hypophyse reste riche en matériel coloré par l'hématoxyline chromique. On peut admettre, à la lumière de résultats antérieurs ou non publiés, que cette hyperactivité des neurones hypothalamiques est liée à une hypersécrétion d'ADH (hormone antidiurétique). L'origine de cette réponse antidiurétique semble complexe: l'activation corticosurrénalienne observée simultanément, et classiquement interprétable comme une conséquence de la stimulation hypothalamique, peut aussi, d'après les travaux de nombreux auteurs, en être partiellement la cause. On note corrélativement l'absence d'oligurie, mais au contraire parfois une polyurie modérée; ceci suggère l'idée d'une réponse antidiurétique consécutive à un trouble du métabolisme hydrique induit par d'autres facteurs polyuriques; mais l'ADH déchargée ne semblerait pas capable de contrebalancer complètement cette tendance polyurique. Par ailleurs il a été prouvé que l'aéro-ionisation négative entraine une baisse du taux sanguin de sérotonine, dont les propriétés antidiurétiques sont connues. Ceci permet d'expliquer une composante polyurique favorisant une réponse hypothalamique antidiurétique. L'ensemble des modifications du métabolisme hydro-minéral observées après aéro-ionisation négative (pertes d'eau et de potassium, sodium inchangé) peuvent s'expliquer de façon satisfaisante par l'augmentation des décharges de l'hormone antidiurétique, et des hormones corticosurrénaliennes, contrebalancée partiellement par une chute concomitante du taux de sérotonine.
    Notes: Summary Supraoptic and paraventricular nuclei in the hypothalamus of albino rats submitted to negative air ions for 3 to 5 weeks exhibit various signs of hyperactivity: a significant increase of nuclear and nucleolar volumes, a decrease of the amount of the neurosecretory granules in these neurones while the migration of the neurosecretory material in the axons is enhanced and becomes clearly visible compared with controls. Simultaneously, the neurohypophysis remains well laden with chrome-haematoxylin positive material. Considering these data, and other results, either unpublished or previously obtained by various authors, it appears that the hyperactivity registered in the hypothalamo-neurohypophysial system may be correlated with a hypersecretion of antidiuretic hormone. The mechanism of this antidiuretic response seems to be rather complex. The adrenocortical stimulation simultaneously detected most often is interpreted as a consequence of a hypothalamic hyperactivity. However, regarding other results, another possibility occurs: that of a role played by the adrenal cortex stimulating the hypothalamus. The feedback between the two hormonal active organs seems to be resetted at a higher level. No antidiuresis could be observed, instead, sometimes a slight increase of diuresis was noted. It appears that the released antidiuretic hormone is unable to counterbalance completely this water metabolism disturbance probably correlated with other factors. Besides, it was established that a treatment with negative air ions induces a significant diminution of the serotonin level of the blood. As serotonin exerts an antidiuretic effect, the antidiuretic response of the hypothalamus would also be enhanced by this decrease of serotonin. All the available data on the modifications of the water and electrolyte metabolism observed after a negative aero-ionization (water and potassium losses, whereas sodium is not clearly affected) may be explained satisfactorily by a simultaneous increase of the discharge of antidiuretic and adrenocortical hormones partly counteracted by a decrease of the serotonin level of the blood.
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    Cell & tissue research 105 (1970), S. 303-316 
    ISSN: 1432-0878
    Keywords: Eye ; Arthropods ; Neurosecretion ; Microscopy, Electron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The lateral rudimentary eye of Limulus polyphemus, the horseshoe crab, is located beneath the posterior border of the compound eye. It consists of a bipartite mass of guanophores and about 100 associated photoreceptor cells. These neurons, up to 150 μ in diameter, have standard attributes of arthropod retinula cells and send large, uninterrupted axons to the brain. Their cytoplasm contains conspicuous clumps of residual bodies and variable, but usually extensive, masses of glycogen and glycoprotein. Hence, these neurons are not neurosecretory in the strict sense, notwithstanding axonal transport of glycogen masses toward the brain. Efferent axons to the rudimentary eye terminate in synaptoid fashion on the axon hillock of sensory cells. Since the rudimentary eye does not transmit impulses to the brain, but is photosensitive, its function may reside in a metabolic responsiveness to long-term changes in illumination.
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  • 86
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    Cell & tissue research 103 (1970), S. 129-148 
    ISSN: 1432-0878
    Keywords: Enteropneusts ; Nerve fibre ; Neurocord ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nerve fibre layer and the neurocord of the Enteropneusts Saccoglossus horsti, Harrimania kupfferi and Ptychodera flava have been examined with the electron microscope. The nerve fibres vary in diameter between 0.15 to 10 μm. The majority of the fibres are of the smaller diameters. The nerve fibre layer is intraepidermal, and is divided by processes running radially from the epithelial cells to the basement membrane that separates the nerve fibre layer from the muscle cells. The cells of origin of these nerve fibres are situated mainly in the innermost layers of the epidermal cells. The nerve fibre profiles contain numerous vesicles of very varied diameter and contents, together with larger granular inclusions that are also found in the nerve cell bodies. Morphologically recognisable synapses are rare, but the majority of fibres are in intimate contact with one another. Sometimes the mass of fibres is divided into bundles by the epithelial cell processes. The majority of giant fibres are situated near to the basement membrane of the neurocord. The giant fibres also have a varied content of vesicles as well as neurofilaments and neurotubules. The central canal in Ptychodera flava and the remnants of the central canal in Saccoglossus horsti are both lined by columnar cells that bear microvilli as well as cilia with the typical 9 + 2 pattern of tubules. Scattered amongst these cells are mucus secreting cells which open into the cavity of the canal.
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  • 87
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    Cell & tissue research 103 (1970), S. 365-381 
    ISSN: 1432-0878
    Keywords: Insects ; Brain ; Corpora pedunculata ; Mushroom bodies ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Die Stiele der Corpora pedunculata im Gehirn von Acheta domesticus L. weisen eine Zonierung auf, die auf einer bestimmten Gruppierung von Globulizellfasertypen in der Stielsäule beruht. 2. Pilzkörperfremde Fasern durchziehen das Stielneuropil in allen Richtungen. Sie sind besonders klar zu erkennen, wenn sie senkrecht zu den Globulizellfasern verlaufen. 3. Sog. chemische Synapsen treten hauptsächlich in der Zone III, die den größten Teil des Stielvolumens einnimmt, und im basalen Stiel in der Zone IV auf. Außer synaptischen Vesikeln, die sich mit Zinkjodid-Osmiumsäure imprägnieren lassen, finden sich in den Pilzkörperfasern zahlreiche osmiophile Grana vom Typ der „dense core vesicles“. 4. Globulizellfasern sind mit pilzkörperfremden Fasern im Stiel synaptisch verknüpft. Sehr wahrscheinlich konvergieren im unteren Stielteil Globulizellfasern auf postsynaptische extrinsische Fortsätze. Wahrscheinlich haben auch Globulizellfasern untereinander synaptischen Kontakt. 5. Synaptische SpezialStrukturen (elektronendichte Auflagerungen, Massierungen von synaptischen Vesikeln) finden sich größtenteils in den anliegenden Winkeln von zwei benachbarten Axonen. Oftmals kann eine Entscheidung über die Polarisierung der Synapsen nicht getroffen werden. 6. Die verschiedenen Fasertypen und ihre Kontakte über Synapsen werden im Hinblick auf die Funktion der Corpora pedunculata diskutiert, die nach verbreiteter Ansicht assoziative Hirnzentren verkörpern.
    Notes: Summary 1. The stalk of the mushroom-bodies (Corpora pedunculata) of the cricket Acheta domesticus L. is composed of nerve fibers from different types of globuli cells. These fibers are arranged into four groups. 2. Extrinsic nerve fibers run through the pedunculus neuropil in different directions. 3. Synapses occur primarily in stalk zone III and in basic stalk parts of zone IV. Intrinsic nerve fibers of globuli cells contain synaptic vesicles, which stain with ZIO-impregnation (Akert and Sandri, 1968), and dense core vesicles. 4. Intrinsic and extrinsic nerve fibers make synaptic contacts. There is some evidence, that globuli cell fibers interact by chemical synapses too. 5. Special synaptic structures such as electron dense projections and accumulations of vesicles are situated predominantly in the angles of two neighbouring axons. The question of synaptic polarization can not always be resolved. 6. The different fiber types and their synaptic contacts are discussed with respect to the function of the corpora pedunculata. The results support the general view, that mushroom bodies represent an important brain center for association.
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    Cell & tissue research 103 (1970), S. 48-60 
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Glutaraldehyde/PTA ; Synapses ; Octopus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Octopus synaptosomes have been examined after glutaraldehyde fixation and phosphotungstic acid (PTA) staining of non-osmicated tissue. The results concentrate on the appearance of the contact region between the presynaptic component of synaptosomes and their postsynaptic processes. Membranes have a triple-layered appearance, consisting of an electronopaque internal coat, an electrontranslucent band and an electronopaque external coat. Good examples of this are found in synaptosomal, dendritic and axonal membranes. At specialized synaptic contact regions the external coats of the pre- and postsynaptic membranes coalesce to form a prominent synaptic plate, which has a width of 18 nm and is subdivided into zones of varying electronopacity. It is suggested that this plate is formed from the specialized external coat of the postsynaptic membrane and the unspecialized external coat of the presynaptic membrane. Presynaptic spicules extend from the internal coat of the presynaptic membrane. They are closely associated with elements of the presynaptic network. It is suggested that the synaptic plate is probably composed of mucopolysaccharides, while the relation of the plate to acetylcholinesterase is discussed. It is proposed that functional localization at the synapse is less precise in octopus than vertebrates.
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    Cell & tissue research 105 (1970), S. 276-302 
    ISSN: 1432-0878
    Keywords: Pineal organ ; Passer domesticus ; Synapses ; Nervous pathway ; Sympathetic innervation ; Photo-neuro-endocrine organ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Parenchym der Epiphysis cerebri von Passer domesticus kommen Nervenzellen vor. Ihre Neuriten ziehen im langgestreckten Epiphysenstiel zur Commissura habenularum. Im proximalen Endabschnitt des Epiphysenstiels wird ein Teil dieser Fasern myelinisiert. Zwischen die Nervenfasern schieben sich zahlreiche Pinealocytenausläufer; synaptische Bänder helfen die letzteren eindeutig zu identifizieren. Im Bereich der synaptischen Bänder liegen: 1. 300 Å Vesikel, 2. 300 Å Vesikel und 800–1200 Å Granula, 3. nur 800–1200 Å große granulierte Vesikel. Die Tatsache, daß in Pinealocytenausläufern nebeneinander synaptische Bänder und Granula vorkommen, und daß apikal in zilientragenden Zellen ebenfalls Granula nachweisbar sind, spricht dafür, daß bei Passer domesticus ein Pinealzelltyp sensorische und sekretorische Strukturmerkmale besitzen kann. Außerdem werden Kontaktsynapsen beobachtet; ihre praesynaptischen Fasern enthalten die gleichen Strukturelemente wie die Fasern mit synaptischen Bändern. Die Zahl der Mikrofibrillen und Mikrotubuli variiert in den Pinealocytenausläufern, in den postsynaptischen Dendriten und in den Neuriten so stark, daß es mitunter schwierig ist, diese Fortsatztypen einwandfrei zu unterscheiden und die Zahl der zum Gehirn ziehenden Neuriten exakt zu ermitteln. Efferente sympathische Nervenfasern dringen in die Bindegewebssepten der Epiphyse ein. Sie enthalten Granula mit einem Durchmesser von 300–500 Å und 800–1200 Å. Nach Injektion von Nialamid zeigen beide Granulatypen einen elektronendichten Kern. Mikrospektrographisch ist Serotonin und Noradrenalin in diesen Nervenfasern nachweisbar. Das Material dieser Studie enthält keinen fluoreszenzmikroskopischen oder elektronenmikroskopischen Hinweis darauf, daß die sympathischen Nervenfasern durch die Basalmembran in den Zellverband des Epiphysenparenchyms eintreten. Im elektronenmikroskopischen Bild haben manche Pinealocytenausläufer eine Ähnlichkeit mit autonomen Nervenfasern. Die funktionelle Bedeutung der Vogelepiphyse als photo-neuro-endokrines Organ wird diskutiert.
    Notes: Summary The pineal organ of Passer domesticus contains nerve cells within its parenchyma. Axons of the nerve cells run within the elongated stalk of the pineal organ to the habenular commissure. At the proximal end of the stalk, some axons become myelinated. In the stalk, the axons intermingle with pinealocyte processes containing synaptic ribbons. The synaptic ribbons are in contact with (1) vesicles with a diameter of 300 Å; (2) 300 Å diameter vesicles and 800–1,200 Å diameter dense-core granules; or (3) the dense-core granules only. Dense-core granules are also present in pinealocytes with 9+0 type cilia. These results suggest that sensory and secretory structures are present in the same pineal cell type. Furthermore, “conventional” synapses are present between receptor and nerve cells: The presynaptic fibers have the same structure as the fibers containing synaptic ribbons. The numbers of microfibrils and microtubules vary among postsynaptic fibers (dendrites), the pinealocyte processes, and the neurites. Thus it is difficult to obtain an exact count of the number of axons running to the brain. Efferent sympathetic nerve fibers enter the pineal organ associated with the connective tissue surrounding blood vessels. The fibers show granules of 300–500 Å diameter or 800–1,200 Å diameter. After nialamide injection, both types of granules contain a dense core. Microspectrographically serotonin and noradrenaline are demonstrated in the sympathetic nerve fibers. There is no evidence found in the material of this study to suggest that sympathetic nerve fibers perforate the basement membrane and enter the parenchymal cell complexes of the pineal organ. Pinealocyte processes and sympathetic nerve fibers often show a very similar ultrastructural pattern. The role of the avian pineal organ in photo-neuro-endocrine regulation is discussed.
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