ALBERT

Description: Recently, there has been enormous development due to advancements in technology. Industries and enterprises are moving towards a digital system, and the oil and gas industries are no exception. There are several threats and risks in digital systems, which are controlled through cyber-security. For the first time in the theory of fuzzy sets, this research analyzes the relationships between cyber-security and cyber-crimes in the oil and gas sectors. The novel concepts of complex intuitionistic fuzzy relations (CIFRs) are introduced. Moreover, the types of CIFRs are defined and their properties are discussed. In addition, an application is presented that uses the Hasse diagram to make a decision regarding the most suitable cyber-security techniques to implement in an industry. Furthermore, the omnipotence of the proposed methods is explained by a comparative study.

Description: Crowdsourcing is a new mode of value creation in which organizations leverage numerous Internet users to accomplish tasks. However, because these workers have different backgrounds and intentions, crowdsourcing suffers from quality concerns. In the literature, tracing the behavior of workers is preferred over other methodologies such as consensus methods and gold standard approaches. This paper proposes two novel models based on workers’ behavior for task classification. These models newly benefit from time-series features and characteristics. The first model uses multiple time-series features with a machine learning classifier. The second model converts time series into images using the recurrent characteristic and applies a convolutional neural network classifier. The proposed models surpass the current state of-the-art baselines in terms of performance. In terms of accuracy, our feature-based model achieved 83.8%, whereas our convolutional neural network model achieved 76.6%.

Description: During the past year, health care environments have struggled to cope with the various impacts of COVID-19 around the world. Health care facilities need to help strengthen resistance to pathogen threats and provide care for patients and health workers in the safest possible way. Architectural design strategies can play a significant role in infection prevention and control. The current study aims to examine the experiences of health workers with hospital spaces during the COVID-19 pandemic. Identifying the difficulties they face, the present study attempts to shed light on the role of the health care layout configuration in combating pandemics. The authors conducted observations at four hospitals and a series of online semi-structured interviews with 162 health care staff from March to May 2020. The study indicated that space configuration and the hospitalization of patients, layout and circulation of the environment, operation services such as indoor environment conditions, maintenance of health care system, and organizational support for health care staff were the most critical factors affecting infection control in health care environments. The initial zoning and separation of patients were the most effective methods of controlling infection. Hospitals with clustered plan layouts were found to be the most effective buildings for the zoning of COVID-19 patients during the pandemic and for infection control.

Description: The monoamine serotonin, 5-hydroxytryptamine (5-HT), is a remarkable molecule with conserved production in prokaryotes and eukaryotes and a wide range of functions. In the gastrointestinal tract, enterochromaffin cells are the most important source for 5-HT production. Some intestinal bacterial species are also able to produce 5-HT. Besides its role as a neurotransmitter, 5-HT acts on immune cells to regulate their activation. Several lines of evidence indicate that intestinal 5-HT signaling is altered in patients with inflammatory bowel disease. In this review, we discuss the current knowledge on the production, secretion, and signaling of 5-HT in the intestine. We present an inventory of intestinal immune and epithelial cells that respond to 5-HT and describe the effects of these signaling processes on intestinal homeostasis. Further, we detail the mechanisms by which 5-HT could affect inflammatory bowel disease course and describe the effects of interventions that target intestinal 5-HT signaling.

Description: The main objective of this study was to explore the perceptions of LGBTQIA+ students regarding sexual and gender diversity in the university context by (1) identifying conceptions about a being LGBTQIA+ student in the higher education context, (2) researching perceptions of the stigma and discrimination against, and inclusion of LGBTQIA+ students and (3) to recognize discourses and scenarios identified by students in the university context regarding sexual diversity and gender diversity, distinguishing their experiences in the classroom as well as in the university, with their peers and with their professors. This research was based on a quantitative method, the sample consisted of 171 students from the School of Medicine of a public university in the United States in the state of Texas. The results showed that there is currently a greater knowledge of the subject of sexual and gender diversity and of the spaces and resources offered by the university on the subject compared to previous years, however, it is found that knowledge is still limited and that this knowledge may possibly be due to the faculty in which they study.

Description: The COVID-19 pandemic affected consumers’ behavior worldwide. This paper aims to analyze consumers’ sustainable food behavior during the COVID-19 pandemic. The research was based on an online survey during May–October 2020 among 859 Romanian consumers. Principal component analysis and cluster analysis were employed to group the consumers based on their behavior. Furthermore, the binary-logistic regression was used to identify the socio-demographic profile of the identified groups. Based on the cluster analysis, the consumers were grouped into three main groups: indifferent, pro-environment protection, and health concerned. The results indicate a positive attitude towards sustainable food behavior. Consumers’ attitude towards sustainable food choice is mainly influenced by age and education level. The study offers valuable information for future public policy and marketing campaign regarding sustainable food behavior.

Description: Pre-clinical studies have demonstrated the efficacy of mesenchymal stem cells (MSCs) expressing tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) or MSC-TRAIL against several tumors. However, due to the existence of cancer stem cells (CSCs), some tumors, including non-small cell lung cancer (NSCLC), exhibit TRAIL resistance. This study was designed to evaluate the capacity of using first-line chemotherapies including cisplatin, 5-fluorouracil (5-FU) and vinorelbine to act as a chemo-sensitizer on CD133+ (prominin-1 positive) CSCs derived from NSCLC cell lines (A549, H460 and H2170) for the purpose of MSC-TRAIL-induced inhibition. We showed that MSC-TRAIL was resistant to all three chemotherapies compared to the NSCLC cell lines, suggesting that the chemotherapies had little effect on MSC-TRAIL viability. Pre-treatment using either cisplatin or 5-FU, but not with vinorelbine, was able to increase the efficacy of MSC-TRAIL to kill the TRAIL-resistant A549-derived CSCs. The study also demonstrated that both 5-FU and vinorelbine were an effective chemo-sensitizer, used to increase the anti-tumor effect of MSC-TRAIL against H460- and H2170-derived CSCs. Furthermore, pre-treatment using cisplatin was noted to enhance the effect of MSC-TRAIL in H460-derived CSCs; however, this effect was not detected in the H2170-derived CSCs. These findings suggest that a pre-treatment using certain chemotherapies in NSCLC could enhance the anti-tumor effect of MSC-TRAIL to target the CSCs, and therefore the combination of chemotherapies and MSC-TRAIL may serve as a novel approach for the treatment of NSCLC.

Description: With most epidemiological studies focused on poultry, dogs are often overlooked as a reservoir of Campylobacter, even though these animals maintain close daily contact with humans. The present study aimed to obtain a first insight into the presence and characteristics of Campylobacter spp. in different canine populations in Portugal, and to evaluate its zoonotic potential through genomic analysis. From a total of 125 rectal swabs collected from companion (n = 71) and hunting dogs (n = 54) living in two different settings, rural (n = 75) and urban (n = 50), 32 Campylobacter spp. isolates were obtained. Four different Campylobacter species were identified by Multiplex PCR and MALDI-TOF mass spectrometry, of which Campylobacter jejuni (n = 14, 44%) was overall the most frequently found species. Relevant resistance phenotypes were detected in C. jejuni, with 93% of the isolates being resistant to ciprofloxacin, 64% to tetracycline, and 57% to ampicillin, and three isolates being multi-drug-resistant. Comparison of the phenotypic and genotypic traits with human isolates from Portuguese patients revealed great similarity between both groups. Particularly relevant, the wgMLST analysis allowed the identification of isolates from human and dogs without any apparent epidemiological relationship, sharing high genetic proximity. Notwithstanding the limited sample size, considering the high genomic diversity of C. jejuni, the genetic overlap between human and dog strains observed in this study confirmed that the occurrence of this species in dogs is of public health concern, reinforcing the call for a One Health approach.

Description: Charcot-Marie-Tooth disease type 2A (CMT2A) is the most common hereditary axonal neuropathy caused by mutations in MFN2 encoding Mitofusin-2, a multifunctional protein located in the outer mitochondrial membrane. In order to study the effects of a novel MFN2K357T mutation associated with early onset, autosomal dominant severe CMT2A, we generated a knock-in mouse model. While Mfn2K357T/K357T mouse pups were postnatally lethal, Mfn2+/K357T heterozygous mice were asymptomatic and had no histopathological changes in their sciatic nerves up to 10 months of age. However, immunofluorescence analysis of Mfn2+/K357T mice revealed aberrant mitochondrial clustering in the sciatic nerves from 6 months of age, in optic nerves from 8 months, and in lumbar spinal cord white matter at 10 months, along with microglia activation. Ultrastructural analyses confirmed dysmorphic mitochondrial aggregates in sciatic and optic nerves. After exposure of 6-month-old mice to lipopolysaccharide, Mfn2+/K357T mice displayed a higher immune response, a more severe motor impairment, and increased CNS inflammation, microglia activation, and macrophage infiltrates. Overall, ubiquitous Mfn2K357T expression renders the CNS and peripheral nerves of Mfn2+/K357T mice more susceptible to mitochondrial clustering, and augments their response to inflammation, modeling some cellular mechanisms that may be relevant for the development of neuropathy in patients with CMT2A.

Description: Mycotic keratitis is common in warm, humid regions with a varying profile of pathogenic fungi according to geographical origin, socioeconomic status, and climatic condition. Clinical diagnosis can be challenging in difficult cases and those refractory to treatment. Fungal hyphae on microscopic examination and culture isolation have been the gold standard in the laboratory diagnosis of fungal keratitis. A culture isolate of the aetiological fungus is essential to perform antifungal susceptibility testing. As the culture isolation of fungi is time-consuming, causing delays in the initiation of treatment, newer investigative modalities such as in vivo confocal microscopy and molecular diagnostic methods have recently gained popularity. Molecular diagnostic techniques now help to obtain a rapid diagnosis of fungal keratitis. Genomic approaches are based on detecting amplicons of ribosomal RNA genes, with internal transcribed spacers being increasingly adopted. Metagenomic deep sequencing allows for rapid and accurate diagnosis without the need to wait for the fungus to grow. This is also helpful in identifying new emerging strains of fungi causing mycotic keratitis. A custom-tear proteomic approach will probably play an important diagnostic role in future in the management of mycotic keratitis. Positive repeat cultures are being suggested as an important gauge indicative of a poor prognosis. Positive repeat fungal cultures help to modify a treatment regimen by increasing its frequency, providing the addition of another topical and oral antifungal agent along with close follow-up for perforation and identifying need for early therapeutic keratoplasty. The role of collagen crosslinking in the treatment of fungal keratitis is not convincingly established. Rapid detection by multiplex PCR and antifungal susceptibility testing of the pathogenic fungi, adopted into a routine management protocol of fungal keratitis, will help to improve treatment outcome. Early therapy is essential in minimizing damage to the corneal tissue, thereby providing a better outcome. The role of conventional therapy with polyenes, systemic and targeted therapy of antifungal agents, newer azoles and echinocandins in fungal keratitis has been widely studied in recent times. Combination therapy can be more efficacious in comparison to monotherapy. Given the diversity of fungal aetiology, the emergence of new corneal pathogenic fungi with varying drug susceptibilities, increasing the drug resistance to antifungal agents in some genera and species, it is perhaps time to adopt recent molecular methods for precise identification and incorporate antifungal susceptibility testing as a routine.

Description: Aquafaba (AQ) emulsification properties are determined by genetics and seed processing conditions. The physicochemical properties and hydration rates of chickpea (CDC Leader) as a control with proven emulsifying properties were recently reported. Here, we identify correlations between soybean (Backtae, Seoritae, and Jwinunikong) physical, chemical, and hydration properties as well as AQ yield from seed and functional (emulsion and foaming) properties. In addition, a total of 20 compounds were identified by NMR including alcohols (isopropanol, ethanol, methanol), organic acids (lactic acid, acetic acid, succinic acid, citric acid, and malic acid), sugars (glucose, galactose, arabinose, sucrose, raffinose, stachyose), essential nutrients (choline, phosphocholine), amino acids (alanine, glutamine), and polyphenols (resveratrol, glycitin). The process used in this study utilizes a soaking step to hydrate the seed of the selected Korean soybean cultivars. The product, AQ, is an oil emulsifier and foaming agent, which is suitable for use as an egg substitute with improved emulsion/foam formation properties when compared with a chickpea-based AQ.

Description: Ischemic strokes (IS) and spinal cord injuries (SCI) are major causes of disability. RhoA is a small GTPase protein that activates a downstream effector, ROCK. The up-regulation of the RhoA/ROCK pathway contributes to neuronal apoptosis, neuroinflammation, blood-brain barrier dysfunction, astrogliosis, and axon growth inhibition in IS and SCI. Noncoding RNAs (ncRNAs), such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), were previously considered to be non-functional. However, they have attracted much attention because they play an essential role in regulating gene expression in physiological and pathological conditions. There is growing evidence that ROCK inhibitors, such as fasudil and VX-210, can reduce injury in IS and SCI in animal models and clinical trials. Recently, it has been reported that miRNAs are decreased in IS and SCI, while lncRNAs are increased. Inhibiting the Rho/ROCK pathway with miRNAs alleviates apoptosis, neuroinflammation, oxidative stress, and axon growth inhibition in IS and SCI. Further studies are required to explore the significance of ncRNAs in IS and SCI and to establish new strategies for preventing and treating these devastating diseases.

Description: Regardless of the efforts of the European Union, freshwaters are in a state of environmental crisis. The Water Framework Directive has established a basis for the protection and restoration of European inland and coastal waters. In parallel, the Birds and Habitats Directives protect, maintain or restore, at favourable conservation status, selected species and habitats under a representative network of protected areas. Hence, the interplay between the EU regulations is of high scientific interest and practical relevance. In this article, Greece is used as a case study to explore whether anticipated synergies between the Water Framework Directive and the Nature Directives result in a better ecological status in the protected areas than in the non-protected ones. We investigated whether the ecological qualities that are defined by three biological quality elements (BQEs) differ between the WFD monitoring sites that are located within the Natura 2000 protected areas and those that are not. We identified a total of 148 river monitoring sites that are located within the Natura 2000 network, which corresponds to 30% of the WFD monitoring network. By employing ordered logit models for each BQE, we found that the ecological quality has the same likelihood to fail the WFD target of “good” quality for sites that are located within and outside the Natura 2000 protected areas. Our results confirmed our hypothesis that the EU directives have little synergy when it comes to restoration of ecological status of Greek running waters, according to the WFD.

Description: High pressure processing (HPP) represents a non-thermal preservation technique for the gentle treatment of food products. Information about the impact of HPP on lipophilic food ingredients (e.g., carotenoids, vitamin E) is still limited in more complex matrices such as kale. Both the variation of pressure levels (200–600 MPa) and different holding times (5–40 min) served as HPP parameters. Whereas a slightly decreasing solvent extractability mostly correlated with increasing pressure regimes; the extension of holding times resulted in elevated extract concentrations, particularly at high-pressures up to 600 MPa. Surprisingly, slightly increasing bioaccessibility correlated with both elevated pressures and extended holding times, indicating matrix-dependent processes during in vitro digestion, compared to results of extractability. Moreover, the verification of syringe filters for digest filtration resulted in the highest relative recoveries using cellulose acetate and polyvinylidene difluoride membranes. The α-tocopherol equivalent antioxidant capacity (αTEAC) and oxygen radical antioxidant capacity (ORAC) assays of treated kale samples, chopped larger in size, showed increased antioxidant capacities, regarding elevated pressures and extended holding times. Consequently, one may conclude that HPP was confirmed as a gentle treatment technique for lipophilic micronutrients in kale. Nevertheless, it was indicated that sample pre-treatments could affect HP-related processes in food matrices prior to and possibly after HPP.

Description: While the shelterin complex guards and coordinates the mechanism of telomere regulation, deregulation of this process is tightly linked to malignant transformation and cancer. Here, we present the novel finding of a germline stop-gain variant (p.Q199*) in the shelterin complex gene POT1, which was identified in a child with acute myeloid leukemia. We show that the cells overexpressing the mutated POT1 display increased DNA damage and chromosomal instabilities compared to the wildtype counterpart. Protein and mRNA expression analyses in the primary patient cells further confirm that, physiologically, the variant leads to a nonfunctional POT1 allele in the patient. Subsequent telomere length measurements in the primary cells carrying heterozygous POT1 p.Q199* as well as POT1 knockdown AML cells revealed telomeric elongation as the main functional effect. These results show a connection between POT1 p.Q199* and telomeric dysregulation and highlight POT1 germline deficiency as a predisposition to myeloid malignancies in childhood.

Description: Background/Purpose: Over the last two decades, malaria has remained a major public health concern worldwide, especially in developing countries leading to high morbidity and mortality among children. Nigeria is the world most burdened malaria endemic nation, contributing more than a quarter of global malaria cases. This study determined the prevalence of malaria among children at 6–59 months in Nigeria, and the effects of individual and contextual factors. Methods: This study utilized data from 2018 Nigeria Demographic and Health Survey (NDHS) involving a weighted sample size of 10,185 children who were tested for malaria using rapid diagnostic test (RDT). Given the hierarchical structure of the data set, such that children at Level-1 were nested in communities at Level-2, and nested in states and Federal Capital Territory (FCT) at Level-3, multilevel mixed effect logistic regression models were used for the analysis. Results: The proportion of children 6–59 months of age in Nigeria that had malaria fever positive as assessed by RDTs was 35.5% (3418/10,185), (CI: 33.9–37.1). Kebbi State had 77.7%, (CI: 70.2–83.5), which was the highest proportion of 6–59 months who were malaria positive, next in line was Katsina State with 55.5%, (CI: 47.7–63.1). The Federal Capital Territory (FCT), Abuja had the proportion of 29.6%, (CI: 21.6–39.0), malaria positive children of 6–59 months of age. Children between the age of 48 and 59 months were 2.68 times more likely to have malaria fever than children of ages 6–11 months (AOR = 2.68, 95% CI: 2.03–3.54). In addition, children from the rural area (AOR = 2.12, 95% CI: 1.75–2.57), were more likely to suffer from malaria infection compared to children from urban area. Conclusion: The study identified some individual and contextual predictors of malaria among children in Nigeria. These factors identified in this study are potential areas that need to be considered for policy designs and implementations toward control and total elimination of malaria-related morbidity and mortality among children in Nigeria.

Description: Over the last decades, growing interest has been devoted to employees’ perceptions of Human Resource Management Practices because of their positive influence on individual attitudes and behaviors as well as on organizational performance. Furthermore, assuming the mutual benefits coming from a people-based management of the human capital in organizations, both in terms of employees’ increased motivation, engagement and commitment, and consequently enhanced performance and competitive advantage, recent research in the field concentrated on the impact of HRM practices perceptions on some distinctive individual attitudes and behaviors driving the success of organizations especially in times of radical change like the present ones. Moving from these assumptions, the aim of the present study was to examine the relationship between HRM practices perception and objective career success, considering the mediating role played by employability and extra-role behaviors. Participants were 960 Italian employees who filled an online self-report questionnaire available through the web platform Google Forms. The questionnaire encompassed socio-demographic information and self-report scales assessing the study variables. Results showed that HRM practices perception was positively related to employability, objective career success, and extra-role behaviors. Implications for theory and practice, limitations, and future research directions were also discussed.

Description: Traffic congestion and the occurrence of traffic accidents are problems that can be mitigated by applying cooperative adaptive cruise control (CACC). In this work, we used deep reinforcement learning for CACC and assessed its potential to outperform model-based methods. The trade-off between distance-error minimization and energy consumption minimization whilst still ensuring operational safety was investigated. Alongside a string stability condition, robustness against burst errors in communication also was incorporated, and the effect of preview information was assessed. The controllers were trained using the proximal policy optimization algorithm. A validation by comparison with a model-based controller was performed. The performance of the trained controllers was verified with respect to the mean energy consumption and the root mean squared distance error. In our evaluation scenarios, the learning-based controllers reduced energy consumption in comparison to the model-based controller by 17.9% on average.

Description: Nowadays, more and more attention is paid to the development and the intensification of the use of renewable energy sources. Hemp might be an alternative plant for bioenergy production. In this paper, four varieties of Polish industrial hemp (Białobrzeskie, Tygra, Henola, and Rajan) were investigated in order to determine which of them are the most advantageous raw materials for the effective production of bioethanol. At the beginning, physical and chemical pretreatment of hemp biomass was carried out. It was found that the most effective is the alkaline treatment with 2% NaOH, and the biomasses of the two varieties were selected for next stages of research: Tygra and Rajan. Hemp biomass before and after pretreatment was analyzed by FTIR and SEM, which confirmed the effectiveness of the pretreatment. Next, an enzymatic hydrolysis process was carried out on the previously selected parameters using the response surface methodology. Subsequently, the two approaches were analyzed: separated hydrolysis and fermentation (SHF) and a simultaneous saccharification and fermentation (SSF) process. For Tygra biomass in the SHF process, the ethanol concentration was 10.5 g∙L−1 (3.04 m3·ha−1), and for Rajan biomass at the SSF process, the ethanol concentration was 7.5 g∙L−1 (2.23 m3·ha−1). In conclusion, the biomass of Polish varieties of hemp, i.e., Tygra and Rajan, was found to be an interesting and promising raw material for bioethanol production.

Description: A dual-band dual-polarized antenna with high isolation characteristics is proposed for polarimetric synthetic aperture radar (PolSAR) applications. The antenna consists of four dipole antennas and 2 × 2 patch antenna arrays operating at the P-band (450–730 MHz) and Ka-band (34–36 GHz), respectively. The dipole antennas and the patch antenna arrays need dual-linear polarization characteristics to acquire PolSAR data. Improvements in the isolation characteristics at the P-band are achieved by inserting a metamaterial absorber with a fractal geometry between the transmitting (Tx) and receiving (Rx) dipole antennas. Without the absorber, the simulated isolation characteristics between the Tx and Rx antennas are lower than 19.2 dB over the target band. On the other hand, with the absorbers, the simulated isolation characteristics are higher than 23.44 dB over the target band, and remarkable improvement is achieved around the resonance frequency of the absorber. The measured results are in good agreement with the simulated ones, showing that the proposed antenna can be a good candidate for PolSAR applications.

Description: Besides their role in cell metabolism, mitochondria display many other functions. Mitochondrial DNA (mtDNA), the own genome of the organelle, plays an important role in modulating the inflammatory immune response. When released from the mitochondrion to the cytosol, mtDNA is recognized by cGAS, a cGAMP which activates a pathway leading to enhanced expression of type I interferons, and by NLRP3 inflammasome, which promotes the activation of pro-inflammatory cytokines Interleukin-1beta and Interleukin-18. Furthermore, mtDNA can be bound by Toll-like receptor 9 in the endosome and activate a pathway that ultimately leads to the expression of pro-inflammatory cytokines. mtDNA is released in the extracellular space in different forms (free DNA, protein-bound DNA fragments) either as free circulating molecules or encapsulated in extracellular vesicles. In this review, we discussed the latest findings concerning the molecular mechanisms that regulate the release of mtDNA from mitochondria, and the mechanisms that connect mtDNA misplacement to the activation of inflammation in different pathophysiological conditions.

Description: Business organizations all around the globe are looking to expand circular models into their supply chains to harness economic and environmental benefits. Moreover, the act of giving incentives to retailers by the manufacturer is also quite prevalent in the present business environment. These incentives are offered to promote the sales of products of a manufacturer. Therefore, this paper examines the optimal decisions for a dual-retailer closed-loop supply chain (CLSC) in which the manufacturer bestows the credit period to the one retailer (a firm that possesses shallow market penetration and has a higher insistence on the usage of the capital venture), and cash discount to the next retailer (a firm that occupies the market to a greater extent and receives lower thrust on the usage of invested capital) under a non-coordinated system and coordinated systems. This study proposes the mathematical model to determine the optimal decisions of the manufacturer in terms of credit period and cash discount and also compute the optimal decisions of the retailers for their retail prices and order quantities to maximize individual’s profit in the CLSC. Moreover, numerical analysis and sensitivity analysis is performed to get insights into the optimal decisions of the manufacturer and retailers. The results of sensitivity analysis show that credit period and cash discount increases with the rise in price elasticity, and decreases with an increase in cross-price elasticity. The findings also confirm that members of dual-retailer CLSC under coordination and manufacture’s incentive scenario generate higher environmental and economic benefits required to attain sustainability in production and consumption.

Description: The number of cities, or parts of cities, where air quality has been computed using the PMSS 3D model now appears to be sufficient to allow assessment and understanding of performance. Two fields of application explain the growing number of sites: the first is the long-term air quality assessment required in urban areas for any building or road project. The geometric complexity found in such areas can justify the use of a 3D approach, as opposed to Gaussian ones. However, these studies have constraining rules that can make the modelling challenging: several scenarios are needed (current, future with project, future without project), the long-term impact implies a long physical time period to be computed, and the spatial extension of the domain can be large in order to cover the traffic impact zone of the project. The second type of application is dedicated to services and, essentially, to forecasting. As for impact assessments, the modelling can be challenging here because of the extension of the domain if the target area is a whole city. Forecast also adds the constraint of time, as results are requested early, and the constraint of robustness. The CPU amount needed to meet all these requirements is important. It is therefore crucial to optimize all possible parts of the modelling chain in order to limit cost and delay. The sites presented in the article have been modelled with PMSS for long periods. This allows feedback to be provided on different topics: (a) daily forecasts offer an opportunity to increase the robustness of the modelling chain; (b) quantitative validation at air quality measurement stations; (c) comparison of annual impact based on a whole year, and based on a sampling list of dates selected thanks to a classification process; (d) large calculation domains with widespread pollutant emissions offer a great opportunity to qualitatively check and improve model results on numerous geometrical configurations; (e) CPU time variations between different sites provide valuable information to select the best parametrizations, to predict the cost of the services, and to design the needed hardware for a new site.

Description: (1) Background: Obesity (OB) is a frequent co-morbidity in Binge Eating Disorder (BED), suggesting that both conditions share phenotypical features along a spectrum of eating-related behaviors. However, the evidence is inconsistent. This study aimed to comprehensively compare OB-BED patients against OB individuals without BED and healthy, normal-weight controls in general psychopathological features, eating-related phenotypes, and early life experiences. (2) Methods: OB-BED patients (n = 37), OB individuals (n = 50), and controls (n = 44) completed a battery of standardized questionnaires. Responses were analyzed using univariate comparisons and dimensionality reduction techniques (linear discriminant analysis, LDA). (3) Results: OB-BED patients showed the highest scores across assessments (e.g., depression, emotional and stress eating, food cravings, food addiction). OB-BED patients did not differ from OB individuals in terms of childhood traumatization or attachment styles. The LDA revealed a two-dimensional solution that distinguished controls from OB and OB-BED in terms of increasing problematic eating behaviors and attitudes, depression, and childhood adversities, as well as OB-BED from OB groups in terms of emotional eating tendencies and self-regulation impairments. (4) Conclusions: Findings support the idea of a shared spectrum of eating-related disorders but also highlight important distinctions relevant to identifying and treating BED in obese patients.

Description: The effectiveness of mulch treatments on soil quality as well as on the yield and growth rates of bamboo are major considerations and require further attention. The present work was aimed at assessing the impacts of three different mulch materials on soil available nutrients, biochemical traits, and growth patterns of Dendrocalamus latiflorus Munro. We found that relative to the control (CK), bamboo leaves (MB) and organic fertilizers (MF) treatments significantly (P 〈 0.05) increased the number of bamboo shoots (47.5 and 22.7%) and yield (21.4 and 9.1%), respectively. We observed that under MB and MF treatments, the concentrations of soil available nutrients (nitrogen, phosphorus, and potassium) increased and played a key role in the differences in chlorophyll, leaf carbohydrate contents (soluble sugar and starch) and were essential to promote bamboo shoot development. Furthermore, we infer from principal component analysis (PCA), that both MB and MF appear to be a better choice than rice husks (MR) to improve nutrient availability, biochemical traits of the leaves, and increased bamboo shoot productivity. Consequently, we suggest using organic fertilizers and bamboo leaves as mulch materials are effective for soil conservation to attain high-quality bamboo production.

Description: In this paper, we provide a mathematical and statistical methodology using heteroscedastic estimation to achieve the aim of building a more precise mathematical model for complex financial data. Considering a general regression model with explanatory variables (the expected value model form) and the error term (including heteroscedasticity), the optimal expected value and heteroscedastic model forms are investigated by linear, nonlinear, curvilinear, and composition function forms, using the minimum mean-squared error criterion to show the precision of the methodology. After combining the two optimal models, the fitted values of the financial data are more precise than the linear regression model in the literature and also show the fitted model forms in the example of Taiwan stock price index futures that has three cases: (1) before COVID-19, (2) during COVID-19, and (3) the entire observation time period. The fitted mathematical models can apparently show how COVID-19 affects the return rates of Taiwan stock price index futures. Furthermore, the fitted heteroscedastic models also show how COVID-19 influences the fluctuations of the return rates of Taiwan stock price index futures. This methodology will contribute to the probability of building algorithms for computing and predicting financial data based on mathematical model form outcomes and assist model comparisons after adding new data to a database.

Description: Smoke-free pregnancies have long-term health benefits for mothers and babies. This paper quantitatively examines factors associated with smoke-free pregnancies among Aboriginal and Torres Strait Islander women (hereafter Aboriginal women) and qualitatively explores their smoking cessation (SC) experiences during pregnancy. An Aboriginal-led online cross-sectional study on SC was conducted with Aboriginal women and in partnership with Aboriginal communities, between July and October 2020. The present analysis includes participants who made a pregnancy-related quit attempt (N = 103). Chi-squared tests, logistic regression models, and thematic analysis of free-form text responses were performed. The adjusted odds of having smoke-free pregnancies were 4.54 times higher among participants who used Aboriginal Health Services (AHS) (AOR = 4.54, p-value 0.018). Participants living in urban settings had 67% lower odds of having smoke-free pregnancies compared to their regional/remote counterparts (AOR = 0.33, p-value 0.020). Qualitative data revealed strong motivations to reduce tobacco-related harms to the fetus and variability in quitting experiences at different stages of and across pregnancies. Smoking cessation care (SCC) can support Aboriginal women meaningfully if their quitting experiences are considered in SCC development and implementation. Consistent funding for AHS-led SCC is needed to garner health benefits for Aboriginal peoples. More research into urban versus regional/remote differences in maternal SC is recommended.

Description: Strong evidence from studies on primates and rodents shows that changes in pupil diameter may reflect neural activity in the locus coeruleus (LC). Pupillometry is the only available non-invasive technique that could be used as a reliable and easily accessible real-time biomarker of changes in the in vivo activity of the LC. However, the application of pupillometry to preclinical research in rodents is not yet fully standardized. A lack of consensus on the technical specifications of some of the components used for image recording or positioning of the animal and cameras have been recorded in recent scientific literature. In this study, a novel pupillometry system to indirectly assess, in real-time, the function of the LC in anesthetized rodents is presented. The system comprises a deep learning SOLOv2 instance-based fast segmentation framework and a platform designed to place the experimental subject, the video cameras for data acquisition, and the light source. The performance of the proposed setup was assessed and compared to other baseline methods using a validation and an external test set. In the latter, the calculated intersection over the union was 0.93 and the mean absolute percentage error was 1.89% for the selected method. The Bland–Altman analysis depicted an excellent agreement. The results confirmed a high accuracy that makes the system suitable for real-time pupil size tracking, regardless of the pupil’s size, light intensity, or any features typical of the recording process in sedated mice. The framework could be used in any neurophysiological study with sedated or fixed-head animals.

Description: Parkinson’s disease (PD) is characterized by the progressive accumulation of neuronal intracellular aggregates largely composed of alpha-Synuclein (αSyn) protein. The process of αSyn aggregation is induced during aging and enhanced by environmental stresses, such as the exposure to pesticides. Paraquat (PQ) is an herbicide which has been widely used in agriculture and associated with PD. PQ is known to cause an increased oxidative stress in exposed individuals but the consequences of such stress on αSyn conformation remains poorly understood. To study αSyn pathogenic modifications in response to PQ, we exposed Drosophila expressing human αSyn to a chronic PQ protocol. We first showed that PQ exposure and αSyn expression synergistically induced fly mortality. The exposure to PQ was also associated with increased levels of total and phosphorylated forms of αSyn in the Drosophila brain. Interestingly, PQ increased the detection of soluble αSyn in highly denaturating buffer but did not increase αSyn resistance to proteinase K digestion. These results suggest that PQ induces the accumulation of toxic soluble and misfolded forms of αSyn but that these toxic forms do not form fibrils or aggregates that are detected by the proteinase K assay. Collectively, our results demonstrate that Drosophila can be used to study the effect of PQ or other environmental neurotoxins on αSyn driven pathology.

Description: Diabetes cardiomyopathy is one of the key factors of mortality among diabetic patients around the globe. One of the prior contributors to the progression of diabetic cardiomyopathy is cardiac mitochondrial dysfunction. The cardiac mitochondrial dysfunction can induce oxidative stress in cardiomyocytes and was found to be the cause of majority of the heart morphological and dynamical changes in diabetic cardiomyopathy. To slow down the occurrence of diabetic cardiomyopathy, it is crucial to discover therapeutic agents that target mitochondrial-induced oxidative stress. Flavonoid is a plentiful phytochemical in plants that shows a wide range of biological actions against human diseases. Flavonoids have been extensively documented for their ability to protect the heart from diabetic cardiomyopathy. Flavonoids’ ability to alleviate diabetic cardiomyopathy is primarily attributed to their antioxidant properties. In this review, we present the mechanisms involved in flavonoid therapies in ameliorating mitochondrial-induced oxidative stress in diabetic cardiomyopathy.

Description: The spatiotemporal evolution of vegetation and its influencing factors can be used to explore the relationships among vegetation, climate change, and human activities, which are of great importance for guiding scientific management of regional ecological environments. In recent years, remote sensing technology has been widely used in dynamic monitoring of vegetation. In this study, the normalized difference vegetation index (NDVI) and standardized precipitation–evapotranspiration index (SPEI) from 1998 to 2017 were used to study the spatiotemporal variation of NDVI in China. The influences of climate change and human activities on NDVI variation were investigated based on the Mann–Kendall test, correlation analysis, and other methods. The results show that the growth rate of NDVI in China was 0.003 year−1. Regions with improved and degraded vegetation accounted for 71.02% and 22.97% of the national territorial area, respectively. The SPEI decreased in 60.08% of the area and exhibited an insignificant drought trend overall. Human activities affected the vegetation cover in the directions of both destruction and restoration. As the elevation and slope increased, the correlation between NDVI and SPEI gradually increased, whereas the impact of human activities on vegetation decreased. Further studies should focus on vegetation changes in the Continental Basin, Southwest Rivers, and Liaohe River Basin.

Description: Synthetic ligands of peroxisome-proliferator-activated receptor beta/delta (PPARβ/δ) are being used as performance-enhancing drugs by athletes. Since we previously showed that PPARβ/δ activation affects T cell biology, we wanted to investigate whether a specific blood T cell signature could be employed as a method to detect the use of PPARβ/δ agonists. We analyzed in primary human T cells the in vitro effect of PPARβ/δ activation on fatty acid oxidation (FAO) and on their differentiation into regulatory T cells (Tregs). Furthermore, we conducted studies in mice assigned to groups according to an 8-week exercise training program and/or a 6-week treatment with 3 mg/kg/day of GW0742, a PPARβ/δ agonist, in order to (1) determine the immune impact of the treatment on secondary lymphoid organs and to (2) validate a blood signature. Our results show that PPARβ/δ activation increases FAO potential in human and mouse T cells and mouse secondary lymphoid organs. This was accompanied by increased Treg polarization of human primary T cells. Moreover, Treg prevalence in mouse lymph nodes was increased when PPARβ/δ activation was combined with exercise training. Lastly, PPARβ/δ activation increased FAO potential in mouse blood T cells. Unfortunately, this signature was masked by training in mice. In conclusion, beyond the fact that it is unlikely that this signature could be used as a doping-control strategy, our results suggest that the use of PPARβ/δ agonists could have potential detrimental immune effects that may not be detectable in blood samples.

Description: Remotely sensed vegetation indices (VIs) have been widely used to estimate the aboveground biomass (AGB) carbon stock of coastal wetlands by establishing Vis-related linear models. However, these models always have high uncertainties due to the large spatial variation and fragmentation of coastal wetlands. In this paper, an efficient coastal wetland AGB model for the Bohami Rim coastal wetlands was presented based on multiple data sets. The model was developed statistically with 7 independent variables from 23 metrics derived from remote sensing, topography, and climate data. Compared to previous models, it had better performance, with a root mean square error and r value of 188.32 g m−2 and 0.74, respectively. Using the model, we firstly generated a regional coastal wetland AGB map with a 10 m spatial resolution. Based on the AGB map, the AGB carbon stock of the Bohai Rim coastal wetland was 2.11 Tg C in 2019. The study demonstrated that integrating emerging high spatial resolution multi-remote sensing data and several auxiliary metrics can effectively improve VIs-based coastal wetland AGB models. Such models with emerging freely available data sets will allow for the rapid monitoring and better understanding of the special role that “blue carbon” plays in global carbon cycle.

Description: Dittrichia viscosa plants were grown hydroponically with different concentrations of Sb. There was preferential accumulation of Sb in roots. Fe and Cu decreased, while Mn decreased in roots but not in leaves. Chlorophyll content declined, but the carotenoid content increased, and photosynthetic efficiency was unaltered. O2●− generation increased slightly, while lipid peroxidation increased only in roots. H2O2, NO, ONOO−, S-nitrosothiols, and H2S showed significant increases, and the enzymatic antioxidant system was altered. In roots, superoxide dismutase (SOD) and monodehydroascorbate reductase (MDAR) activities declined, dehydroscorbate reductase (DHAR) rose, and ascorbate peroxidase (APX), peroxidase (POX), and glutathione reductase (GR) were unaffected. In leaves, SOD and POX increased, MDAR decreased, and APX was unaltered, while GR increased. S-nitrosoglutathione reductase (GSNOR) and l-cysteine desulfhydrilase (l-DES) increased in activity, while glutathione S-transferase (GST) decreased in leaves but was enhanced in roots. Components of the AsA/GSH cycle decreased. The great capacity of Dittrichia roots to accumulate Sb is the reason for the differing behaviour observed in the enzymatic antioxidant systems of the two organs. Sb appears to act by binding to thiol groups, which can alter free GSH content and SOD and GST activities. The coniferyl alcohol peroxidase activity increased, possibly to lignify the roots’ cell walls. Sb altered the ROS balance, especially with respect to H2O2. This led to an increase in NO and H2S acting on the antioxidant system to limit that Sb-induced redox imbalance. The interaction NO, H2S and H2O2 appears key to the response to stress induced by Sb. The interaction between ROS, NO, and H2S appears to be involved in the response to Sb.

Description: Methionine restriction (MetR) is an efficient method of amino acid restriction (AR) in cells and organisms that induces low energy metabolism (LEM) similar to caloric restriction (CR). The implementation of MetR as a therapy for cancer or other diseases is not simple since the elimination of a single amino acid in the diet is difficult. However, the in vivo turnover rate of cysteine is usually higher than the rate of intake through food. For this reason, every cell can enzymatically synthesize cysteine from methionine, which enables the use of specific enzymatic inhibitors. In this work, we analysed the potential of cysteine restriction (CysR) in the murine cell line L929. This study determined metabolic fingerprints using mass spectrometry (LC/MS). The profiles were compared with profiles created in an earlier work under MetR. The study was supplemented by proliferation studies using D-amino acid analogues and inhibitors of intracellular cysteine synthesis. CysR showed a proliferation inhibition potential comparable to that of MetR. However, the metabolic footprints differed significantly and showed that CysR does not induce classic LEM at the metabolic level. Nevertheless, CysR offers great potential as an alternative for decisive interventions in general and tumour metabolism at the metabolic level.

Description: Non-coding RNAs (ncRNAs) are emerging therapeutic tools but there are barriers to their translation to clinical practice. Key issues concern the specificity of the targets, the delivery of the molecules, and their stability, while avoiding “on-target” and “off-target” side effects. In this “ncRNA in therapeutics” issue, we collect several studies of the differential expression of ncRNAs in cardiovascular diseases, bone metabolism-related disorders, neurology, and oncology, and their potential to be used as biomarkers or therapeutic targets. Moreover, we review recent advances in the use of antisense ncRNAs in targeted therapies with a particular emphasis on their basic biological mechanisms, their translational potential, and future trends.

Description: Glioblastoma (GBM) is one of the deadliest of all human cancers. Developing therapies targeting GBM cancer stem cells or glioma stem cells (GSCs), which are deemed responsible for the malignancy of GBM due to their therapy resistance and tumor-initiating capacity, is considered key to improving the dismal prognosis of GBM patients. In this study, we found that folate antagonists, such as methotrexate (MTX) and pemetrexed, are selectively cytotoxic to GSCs, but not to their differentiated counterparts, normal fibroblasts, or neural stem cells in vitro, and that the high sensitivity of GCSs to anti-folates may be due to the increased expression of RFC-1/SLC19A1, the reduced folate carrier that transports MTX into cells, in GSCs. Of note, in an in vivo serial transplantation model, MTX alone failed to exhibit anti-GSC effects but promoted the anti-GSC effects of CEP1347, an inducer of GSC differentiation. This suggests that folate metabolism, which plays an essential role specifically in GSCs, is a promising target of anti-GSC therapy, and that the combination of cytotoxic and differentiation therapies may be a novel and promising approach to effectively eliminate cancer stem cells.

Description: Aquaculture has grown rapidly in the field of food industry in recent years; however, it brought many environmental problems, such as water pollution and reclamations of lakes and coastal wetland areas. Thus, the evaluation and management of aquaculture industry are needed, in which accurate aquaculture mapping is an essential prerequisite. Due to the difference between inland and marine aquaculture areas and the difficulty in processing large amounts of remote sensing images, the accurate mapping of different aquaculture types is still challenging. In this study, a novel approach based on multi-source spectral and texture features was proposed to map simultaneously inland and marine aquaculture areas. Time series optical Sentinel-2 images were first employed to derive spectral indices for obtaining texture features. The backscattering and texture features derived from the synthetic aperture radar (SAR) images of Sentinel-1A were then used to distinguish aquaculture areas from other geographical entities. Finally, a supervised Random Forest classifier was applied for large scale aquaculture area mapping. To address the low efficiency in processing large amounts of remote sensing images, the proposed approach was implemented on the Google Earth Engine (GEE) platform. A case study in the Pearl River Basin (Guangdong Province) of China showed that the proposed approach obtained aquaculture map with an overall accuracy of 89.5%, and the implementation of proposed approach on GEE platform greatly improved the efficiency for large scale aquaculture area mapping. The derived aquaculture map may support decision-making services for the sustainable development of aquaculture areas and ecological protection in the study area, and the proposed approach holds great potential for mapping aquacultures on both national and global scales.

Description: Plicosepalus acacia (Fam. Loranthaceae) has been reported to possess hypoglycemic, antioxidant, antimicrobial, and anti-inflammatory effects. Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) analysis revealed the presence of a high content of polyphenolic compounds that are attributed to the therapeutic effects of the crude extract. In addition, methyl gallate and quercetin were detected as major phytomedicinal agents at concentrations of 1.7% and 0.062 g%, respectively, using high-performance thin layer chromatography (HPTLC). The present study investigated the effect of the P. acacia extract and its isolated compounds, methyl gallate and quercetin, on hind limb ischemia induced in type 1 diabetic rats. Histopathological examination revealed that treatment with P. acacia extract, methyl gallate, and quercetin decreased degenerative changes and inflammation in the ischemic muscle. Further biochemical assessment of the hind limb tissue showed decreased oxidative stress, increased levels of nitric oxide and endothelial nitric oxide synthase (eNOS), and enhancement of the levels of heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) in the groups treated with methyl gallate and quercetin. Expression levels of hypoxia inducible factor-1 alpha (HIF-1α), VEGF, fibroblast growth factor-2 (FGF-2), and miR-146a were upregulated in the muscle tissue of methyl gallate- and quercetin-treated groups along with downregulation of nuclear factor kappa B (NF-κB). In conclusion, P. acacia extract and its isolated compounds, methyl gallate and quercetin, mediated therapeutic angiogenesis in diabetic hind limb ischemia.

Description: In China, peanut sprouts are popular among consumers as functional vegetables. This study reports the change in total phenolic content (TPC), total flavonoid content (TFC), monomeric anthocyanin content (MAC), vitamin C, trans-resveratrol content, antioxidant capacities, and phenolic profile of three different varieties of peanut during 8 days of germination. The TPC, TFC, and antioxidant capacity of peanut samples were reduced and then increased with an increase in germination time. TFC values were highly correlated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) values. MAC values of peanuts were first increased and then decreased during 8 days of germination. The TFC, DPPH, and FRAP values of germinated peanuts were lower compared to the non-germinated peanut. Germination of peanut samples enhanced the total phenolic acids and trans-resveratrol content, but the vitamin C content of peanut sprouts was lower than ungerminated peanuts.

Description: Cholera remains a heavy burden worldwide, especially in Sub-Saharan African countries, which account for the majority of the reported cases on the continent. In this study, a 27-year retrospective analysis of cholera epidemics in Guinea-Bissau was performed in order to highlight major stressors fueling the trigger and dissemination of the disease. Although the role of environmental factors did not always have the same degree of importance for the onset of epidemics, a cholera seasonal pattern was clearly perceived, with most of the reported cases occurring during the wet season. The generated theoretical hypothesis indicated rainfall above climatological average, associated with a lack of WASH (water, sanitation and hygiene) infrastructure, and the occurrence of concomitant epidemics in neighboring countries as the key indicators for optimal conditions for cholera to thrive in Guinea-Bissau. Warmer air temperature, the increase in sea surface temperature, and the decrease in salinity in the coastal areas may also contribute to the emergence and/or aggravation of cholera events. Prediction of the conditions favorable for cholera growth and identification of risk pathways will allow the timely allocation of resources, and support the development of alert tools and mitigation strategies.

Description: Copolymers based on 4,4′-dihydroxydiphenyl, phenolphthalein and 4,4′-dichlorodiphenyl sulfone were synthesized by the method of high temperature polycondensation. The structure of the synthesized copolymers was confirmed by IR spectroscopy. Their physical, mechanical and thermal properties were investigated. It is shown that increases in the content of carded fragments lead to higher glass transition temperatures and heat resistance of the copolymers, as well as higher elastic and strength properties.

Description: PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Initially described as a regulator of chromatin dynamics through the methylation of histone H4 at arginine 3 (H4R3), numerous non-histone substrates have since been identified. The variety of these substrates underlines the essential role played by PRMT1 in a large number of biological processes such as transcriptional regulation, signal transduction or DNA repair. This review will provide an overview of the structural, biochemical and cellular features of PRMT1. After a description of the genomic organization and protein structure of PRMT1, special consideration was given to the regulation of PRMT1 enzymatic activity. Finally, we discuss the involvement of PRMT1 in embryonic development, DNA damage repair, as well as its participation in the initiation and progression of several types of cancers.

Description: Although remote sensors have been increasingly providing dense data and deriving reanalysis data for inversion of particulate matters, the use of these data is considerably limited by the ground monitoring samples and conventional machine learning models. As regional criteria air pollutants, particulate matters present a strong spatial correlation of long range. Conventional machine learning cannot or can only model such spatial pattern in a limited way. Here, we propose a method of a geographic graph hybrid network to encode a spatial neighborhood feature to make robust estimation of coarse and fine particulate matters (PM10 and PM2.5). Based on Tobler’s First Law of Geography and graph convolutions, we constructed the architecture of a geographic graph hybrid network, in which full residual deep layers were connected with graph convolutions to reduce over-smoothing, subject to the PM10–PM2.5 relationship constraint. In the site-based independent test in mainland China (2015–2018), our method achieved much better generalization than typical state-of-the-art methods (improvement in R2: 8–78%, decrease in RMSE: 14–48%). This study shows that the proposed method can encode the neighborhood information and can make an important contribution to improvement in generalization and extrapolation of geo-features with strong spatial correlation, such as PM2.5 and PM10.

Description: Although the negative effect of social media use among youth on body image and eating concerns has been established, few classroom-based resources that can decrease these effects through targeting social media literacy skills have been developed. This study aimed to test the efficacy of SoMe, a social media literacy body image, dieting, and wellbeing program for adolescents, through a cluster randomized controlled trial. Participants (n = 892; Mage = 12.77, SD = 0.74; range 11–15; 49.5% male) were randomized by school (n = 8) to receive either weekly SoMe (n = 483) or control sessions (lessons as usual; n = 409) over 4 weeks in their classroom. Participants completed surveys at four timepoints (baseline, 1-week post-intervention, and 6- and 12-month follow-up) assessing body dissatisfaction, dietary restraint, strategies to increase muscles (primary outcomes), self-esteem and depressive symptoms (secondary outcomes), and internalization of appearance ideals and appearance comparison (exploratory outcomes). Modest positive intervention effects were found in dietary restraint and depressive symptoms at 6-month follow-up in girls but few positive effects emerged for boys. The findings provide only preliminary support for a social media literacy intervention, but suggest the usefulness of both identifying those who benefit most from a universally delivered intervention and the need to refine the intervention to maximize intervention effects.

Description: Photocatalysis is proven to be the most efficient and environmentally friendly method for the degradation of organic pollutants in water purification. To meet the requirement of large-scale water treatment, there are two important points: One is the lifetime and chemical stability of the photocatalyst material, especially in the complex and harsh aqueous conditions. The other is the ease of synthesis of such photocatalysts with specific nano-morphology. In this work, two common photocatalyst materials, zinc oxide (ZnO) and titanium dioxide (TiO2), are selected to form more sustainable photocatalysts with high chemical stability. This involves the combination of both TiO2 and ZnO in a two-step simple synthesis method. It appears advantageous to exploit the conformal deposition of atomic layer deposition (ALD) to achieve nanometer-thick TiO2 coating on ZnO nanowires (NWs) with a high aspect ratio, which are firmly anchored to a substrate and exhibit a large specific surface area. The high chemical stability of the ALD TiO2 coating has been investigated in detail and proven to be effective under both strong acid and strong alkaline aqueous solutions. In addition, photocatalysis experiments with organic dyes show that via this simple two-step synthesis method, the produced ZnO/TiO2 tandem photocatalysts does indeed exhibit improved chemical stability in a harsh environment, while allowing efficient photodegradation.

Description: This study focused on the reduction of the treatment cost of mature landfill leachate (LL) by enhancing the coagulation pre-treatment before a UVA-LED photo-Fenton process. A more efficient advanced coagulation pretreatment was designed by combining conventional coagulation (CC) and electro-coagulation (EC). Regardless of the order in which the two coagulations were applied, the combination achieved more than 73% color removal, 80% COD removal, and 27% SUVA removal. However, the coagulation order had a great influence on both final pH and total dissolved iron, which were key parameters for the UVA-LED photo-Fenton post-treatment. CC (pH = 5; 2 g L−1 of FeCl36H2O) followed by EC (pH = 5; 10 mA cm−2) resulted in a pH of 6.4 and 100 mg L−1 of dissolved iron, whereas EC (pH = 4; 10 mA cm−2) followed by CC (pH = 6; 1 g L−1 FeCl36H2O) led to a final pH of 3.4 and 210 mg L−1 dissolved iron. This last combination was therefore considered better for the posterior photo-Fenton treatment. Results at the best cost-efficient [H2O2]:COD ratio of 1.063 showed a high treatment efficiency, namely the removal of 99% of the color, 89% of the COD, and 60% of the SUVA. Conductivity was reduced by 17%, and biodegradability increased to BOD5:COD = 0.40. With this proposed treatment, a final COD of only 453 mg O2 L−1 was obtained at a treatment cost of EUR 3.42 kg COD−1.

Description: The process of spermatogenesis is complex and systemic, requiring the cooperation of many regulators. However, little is known about how micro RNAs (miRNAs) regulate spermatogenesis in poultry. In this study, we investigated key miRNAs and their target genes that are involved in spermatogenesis in chickens. Next-generation sequencing was conducted to determine miRNA expression profiles in five cell types: primordial germ cells (PGCs), spermatogonial stem cells (SSCs), spermatogonia (Spa), and chicken sperm. Next, we analyzed and identified several key miRNAs that regulate spermatogenesis in the four germline cell miRNA profiles. Among the enriched miRNAs, miRNA-301a-5p was the key miRNA in PGCs, SSCs, and Spa. Through reverse transcription quantitative PCR (RT-qPCR), dual-luciferase, and miRNA salience, we confirmed that miR-301a-5p binds to transforming growth factor-beta 2 (TGFβ2) and is involved in the transforming growth factor-beta (TGF-β) signaling pathway and germ cell development. To the best of our knowledge, this is the first demonstration of miR-301a-5p involvement in spermatogenesis by direct binding to TGFβ2, a key gene in the TGF-β signaling pathway. This finding contributes to the insights into the molecular mechanism through which miRNAs regulate germline cell differentiation and spermatogenesis in chickens.

Description: A new transducer based on a screen-printed carbon electrode has been developed for the quantification of thiram. Detection of this fungicide is based on the performance of two enzymes: (1) aldehyde dehydrogenase catalyzes the aldehyde oxidation using NAD+ as a cofactor and simultaneously, (2) diaphorase reoxidizes the NADH formed in the first enzymatic process due to the presence of hexacyanoferrate(III) which is reduced to hexacyanoferrate(II). Taking into account that aldehyde dehydrogenase is inhibited by thiram, the current decreases with pesticide concentration and thiram can be electrochemically quantified below legal limits. The transducer proposed in this work involves the modification of the carbon WE with the co-factors (NAD+ and hexacyanoferrate(III)) required in the enzymatic system. The new device employed in this work allows the detection of 0.09 ppm thiram, a concentration below legal limits (Maximum Residue Limits 0.1–10 ppm).

Description: White adipocytes contribute to energy storage, accumulating lipid droplets, whereas brown and beige adipocytes mainly function in dissipating energy as heat primarily via the action of uncoupling protein 1 (UCP1). Bone morphogenic protein 7 (BMP7) was shown to drive brown adipocyte differentiation in murine interscapular adipose tissue. Here, we performed global RNA-sequencing and functional assays on adipocytes obtained from subcutaneous (SC) and deep-neck (DN) depots of human neck and differentiated with or without BMP7. We found that BMP7 did not influence differentiation but upregulated browning markers, including UCP1 mRNA and protein in SC and DN derived adipocytes. BMP7 also enhanced mitochondrial DNA content, levels of oxidative phosphorylation complex subunits, along with PGC1α and p-CREB upregulation, and fragmentation of mitochondria. Furthermore, both UCP1-dependent proton leak and UCP1-independent, creatine-driven substrate cycle coupled thermogenesis were augmented upon BMP7 addition. The gene expression analysis also shed light on the possible role of genes unrelated to thermogenesis thus far, including ACAN, CRYAB, and ID1, which were among the highest upregulated ones by BMP7 treatment in both types of adipocytes. Together, our study shows that BMP7 strongly upregulates thermogenesis in human neck area derived adipocytes, along with genes, which might have a supporting role in energy expenditure.

Description: Macadamia (Macadamia integrifolia) is endemic to eastern Australia and produces an edible nut that is widely cultivated in commercial orchards globally. A survey of fungi associated with the grey and green mold symptoms of macadamia flowers found mostly species of Botrytis (Sclerotiniaceae, Leotiomycetes) and Cladosporium (Cladosporiaceae, Dothideomycetes). These isolates included B. cinerea, C. cladosporioides, and unidentified isolates. Amongst the unidentified isolates, one novel species of Botrytis and three novel species of Cladosporium were delimited and characterized by molecular phylogenetic analyses. The new species are Botrytis macadamiae, Cladosporium devikae, C. macadamiae, and C. proteacearum.

Description: Graphene, synthesized either epitaxially on silicon carbide or via chemical vapor deposition (CVD) on a transition metal, is gathering an increasing amount of interest from industrial and commercial ventures due to its remarkable electronic, mechanical, and thermal properties, as well as the ease with which it can be incorporated into devices. To exploit these superlative properties, it is generally necessary to transfer graphene from its conductive growth substrate to a more appropriate target substrate. In this review, we analyze the literature describing graphene transfer methods developed over the last decade. We present a simple physical model of the adhesion of graphene to its substrate, and we use this model to organize the various graphene transfer techniques by how they tackle the problem of modulating the adhesion energy between graphene and its substrate. We consider the challenges inherent in both delamination of graphene from its original substrate as well as relamination of graphene onto its target substrate, and we show how our simple model can rationalize various transfer strategies to mitigate these challenges and overcome the introduction of impurities and defects into the graphene. Our analysis of graphene transfer strategies concludes with a suggestion of possible future directions for the field.

Description: A basic assumption of many scientific theories on the topic of decision making is rational behaviour. However, previous authors assume the existence of behavioural biases in freight transport which impede rational decision making. Hardly any research exists on behavioural biases in freight transport. To address this gap, we carry out a systematic literature review on the influencing factors of freight mode choice and provide empirical evidence for the occurrence of behavioural biases in the logistics sector. Fifteen logistics service providers and six shippers are involved in interviews and a focus group to understand their mode choice process and derive information on the existence of behavioural biases. Several biases showed to exist in the practical decision-making process. For example, decision makers tend to avoid complex options (principle of least effort), they stick to already tried and tested options (status quo bias, zero-risk bias) and they tend to make decisions based on immediate and easily accessible information (availability bias). These biases distort the demand for sustainable freight transport. We therefore conclude with several motivational, cognitive and technological debiasing strategies to reduce the negative impact of behavioural biases in freight transport.

Description: Railway vehicle makers manufacture the bogie frame by welding medium-strength carbon steel sheets. It has been a long-standing practice to perform post-weld heat treatment (PWHT) to remove welding-residual stress, but rail car manufacturers are moving toward producing bogie frames without PWHT. Since securing the fatigue strength of the bogie frame is essential for vehicle operation safety, it is necessary to systematically evaluate the effects of PWHT on hardness, microstructure, mechanical properties, corrosion, fatigue strength, etc. In this study, small-scale welding specimens and full-size components were produced using S355JR used in general structures, automobiles, shipbuilding, railroad vehicles, etc. The effect of PWHT on material properties-the hardness of the base material, heat-affected zone and weld metal, microstructure, shock absorption energy, yield strength, tensile strength, and fatigue were investigated. When the weld specimen was annealed at 590 °C and 800 °C for 1 h, the yield strength and tensile strength of the specimen decreased, but the elongation increased. For specimens not heat-treated, the parent material’s yield strength, the yield strength in HAZ, and the yield strength of the weld metal were 350 MPa, 345 MPa, and 340 MPa. For specimens heat-treated at 590 °C, they were 350 MPa, 345 MPa, and 340 MPa. For specimens heat-treated at 800 °C, they were 350 MPa, 345 MPa, and 340 MPa. Annealing heat treatment of the specimen at 800 °C homogenized the structure of the weldments similar to that of the base material and slightly improved the shock absorption energy. For specimens not heat-treated, the Charpy impact absorption energies at 20 °C of the parent material and weld metal were 291.5 J and 187 J. For specimens heat-treated at 590 °C, they were 276 J and 166 J. For specimens heat-treated at 800 °C, the Charpy impact absorption energy at 20 °C of the parent material was 299 J. PWHT at 590 °C had the effect of slightly improving the fatigue limit of the specimen but lowered the fatigue limit by 10.8% for the component specimen.

Description: The electronic and optical properties of polythiophene (PT) for polymer light-emitting diodes (PLEDs) were calculated using density functional theory (DFT) and time-dependent DFT. We calculated the electronic and optical properties of thiophene and PT polymers with degrees of polymerization (DP) from 2 to 30 monomers (T1–T30) and their derivatives. The associated highest occupied molecular orbital (HOMO) energy, lowest unoccupied molecular orbital (LUMO) energy, band gaps, electron orbitals, and molecular structures were determined. As the DP increased, the LUMO energy gradually decreased, and the HOMO energy gradually increased. The band gap of PT approached 2 eV as the DP of the PT polymer increased from 1 to 30. The calculations and exchange–correlation functional were verified against values in the literature and experimental data from cyclic voltammetry (redox potential) and ultraviolet-visible, photoluminescence, and ultraviolet photoelectron spectra. The color of PT PLEDs can be adjusted by controlling the DP of the polymer and the substituents.

Description: This paper presents the results of an experimental program aimed at the assessment of the freeze–thaw (F–T) resistance of concrete based on the evaluation of fracture tests accompanied by acoustic emission measurements. Two concretes of similar mechanical characteristics were manufactured for the experiment. The main difference between the C1 and C2 concrete was in the total number of air voids and in the A300 parameter, where both parameters were higher for C1 by about 35% and 52%, respectively. The evaluation of the fracture characteristics was performed on the basis of experimentally recorded load–deflection and load–crack mouth opening displacement diagrams using two different approaches: linear fracture mechanics completed with the effective crack model and the double-K model. The results show that both approaches gave similar results, especially if the nonlinear behavior before the peak load was considered. According to the results, it can be stated that continuous AE measurement is beneficial for the assessment of the extent of concrete deterioration, and it suitably supplements the fracture test evaluation. A comparison of the results of fracture tests with the resonance method and splitting tensile strength test shows that all testing methods led to the same conclusion, i.e., the C1 concrete was more F–T-resistant than C2. However, the fracture test evaluation provided more detailed information about the internal structure deterioration due to the F–T exposure.

Description: Textile effluent containing azo dyes such as C.I. Acid Violet 1 (AV1) can be degraded to toxic aromatic amines in the environment. Thus, there is a legitimate need to treat such effluents before they are discharged to surface waters. Two methods were proposed to remove AV1 from aqueous solutions: adsorption and advanced oxidation processes (AOPs). The sorption capacity of the strongly basic anion exchanger Purolite A520E of the polystyrene matrix determined from the Langmuir isotherm model was found to be 835 mg/g, while that of Lewatit S5428 of the polyacrylamide matrix Freundlich model seems to be more appropriate for describing the experimental data. The pseudo-second-order kinetic model and external diffusion are the rate limiting steps of adsorption. The removal efficiency of AV1 by the anion exchangers was higher than 99% after 40 min of phase contact time. AOPs involved the usage of hydrogen peroxide and peracetic acid (PAA) as oxidizing agents, while Fe2+ and simulated sunlight were used as oxidizing activators. AV1 oxidation followed the pseudo-first-order kinetics, and the systems with the highest values of the rate constants turned out to be those in which Fe2+ was present. The efficiency of oxidation measured by the degree of decolorization in the systems with Fe2+ was higher than 99% after 10–60 min. AV1 mineralization was slower, but after 120 min of oxidation it was higher than 98% in the H2O2/Fe2+, PAA/Fe2+ and PAA/Fe2+/sunlight systems.

Description: Silver nanoparticles (AgNPs) were successfully synthesized using the aqueous extract of the Paronychia argentea Lam (P. argentea) wild plant. The results showed that the conversion of Ag+ to Ag0 nanoparticles ratio reached 96.5% as determined by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES), with a negative zeta potential (ζ) of −21.3 ± 7.68 mV of AgNPs expected to improve the stability of synthesized AgNPs. AgNP antibacterial activity has been examined against Streptococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria. The minimum inhibition concentration (MIC) was 4.9 µL/mL for both E. coli and S. aureus bacteria, while the minimum bactericidal concentrations (MBC) were 19.9 µL/mL and 4.9 µL/mL for S. aureus and E. coli, respectively. The synthesized AgNPs were incorporated in ultrafiltration polyvinylidene Fluoride (PVDF) membranes and showed remarkable antibiofouling behavior against both bacterial strains. The membranes were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and X-ray diffraction (XRD). The contact angle and porosity of the membrane were also determined. The efficiency of the membranes regarding rejection rate was assessed using bovine serum albumin (BSA). It was found in the flux experiments that membranes BSA rejection was 99.4% and 98.7% with and without AgNPs, respectively.

Description: This paper analyzes the impact of the managerial ability and level of compensation on firm value in small and medium enterprises. The ability of managers is important for the sustainability and growth of small and medium enterprises because they lack awareness of resources, technology, and reputation compared to large enterprises. The managerial ability is the ability to efficiently utilize resources and choose the investment plan with the highest future net cash flows. Managerial ability is also an indicator of the level of compensation for executives. Therefore, the level of executive compensation can help a firm value or growth if it is based on managerial abilities. In addition, high executive compensation standards can be an opportunity or motivation to work hard for the wealth of companies and shareholders. We analyzed 1872 small and medium-sized companies listed on the Korean stock market to achieve the purpose of the research. We analyzed the accounting period of 6 years from 2012 to 2017. Our results have had a positive impact on firm value with executive compensation. In groups with excellent managerial skills, executive compensation has had a positive (+) impact on firm value. However, executive compensation did not have a significant impact on firm value in groups with poor managerial skills. These results validate that the CEO’s role in small and medium enterprise is important and that the level of compensation for executives is important to motivate. It also suggests that executive compensation cannot affect the firm value in groups with low managerial abilities.

Description: The Y Balance Test (YBT) is a dynamic balance assessment typically used in sports medicine. This work proposes a deep learning approach to automatically score this YBT by estimating the normalized reach distance (NRD) using a wearable sensor to register inertial signals during the movement. This paper evaluates several signal processing techniques to extract relevant information to feed the deep neural network. This evaluation was performed using a state-of-the-art human activity recognition system based on recurrent neural networks (RNNs). This deep neural network includes long short-term memory (LSTM) layers to learn features from time series by modeling temporal patterns and an additional fully connected layer to estimate the NRD (normalized by the leg length). All analyses were carried out using a dataset with YBT assessments from 407 subjects, including young and middle-aged volunteers and athletes from different sports. This dataset allowed developing a global and robust solution for scoring the YBT in a wide range of applications. The experimentation setup considered a 10-fold subject-wise cross-validation using training, validation, and testing subsets. The mean absolute percentage error (MAPE) obtained was 7.88 ± 0.20%. Moreover, this work proposes specific regression systems to estimate the NRD for each direction separately, obtaining an average MAPE of 7.33 ± 0.26%. This deep learning approach was compared to a previous work using dynamic time warping and k-NN algorithms, obtaining a relative MAPE reduction of 10%.

Description: Overweight can be an additional problem in patients admitted to hospital. Objective: To analyze gender differences in pre-admission dietary habits and physical exercise and in HRQoL at hospital discharge among hospitalized adults with overweight. Methods: Cross-sectional study in non-diabetic patients enrolled in a clinical trial with body mass index (BMI) ≥ 25 Kg/m2 at admission. Bivariate analyses used Pearson’s chi-square test and Fisher’s exact test for qualitative variables and the Mann–Whitney test for numerical variables. Results: The study included 148 males and 127 females. At admission, women had higher BMI (p = 0.016) than men and a larger percentage consumed drugs for depression (p = 0.030) and anxiety (p = 0.049), and followed a religion-based diet (p = 0.022). Pre-admission, women had healthier habits related to dietary caloric intake (p = 0.009) and greater adherence to recommendations for a healthy diet (p = 0.001). At discharge, women described worse self-perceived health (p = 0.044) and greater pain/discomfort (p = 0.004) in comparison to men. Conclusions: Pre-admission, women had better habits related to a healthy diet and did not differ from men in habits related to physical exercise but had a higher BMI. At discharge, women reported worse self-perceived health and greater pain/discomfort. These differences should be considered for the adequate clinical management of patients with overweight.

Description: Pulmonary premature ageing and fibrogenesis as in idiopathic pulmonary fibrosis (IPF) occur with the DNA damage response in lungs deficient of telomerase. The molecular mechanism mediating pulmonary alveolar cell fates remains to be investigated. The present study shows that naturally occurring ageing is associated with the DNA damage response (DDR) and activation of the p53 signalling pathway. Telomerase deficiency induced by telomerase RNA component (TERC) knockout (KO) accelerates not only replicative senescence but also altered differentiation and apoptosis of the pulmonary alveolar stem cells (AEC2) in association with increased innate immune natural killer (NK) cells in TERC KO mice. TERC KO results in increased senescence-associated heterochromatin foci (SAHF) marker HP1γ, p21, p16, and apoptosis-associated cleaved caspase-3 in AEC2. However, additional deficiency of the tumour suppressor p53 in the Trp53−/− allele of the late generation of TERC KO mice attenuates the increased senescent and apoptotic markers significantly. Moreover, p53 deficiency has no significant effect on the increased gene expression of T1α (a marker of terminal differentiated AEC1) in AEC2 of the late generation of TERC KO mice. These findings demonstrate that, in natural ageing or premature ageing accelerated by telomere shortening, pulmonary senescence and IPF develop with alveolar stem cell p53-dependent premature replicative senescence, apoptosis, and p53-independent differentiation, resulting in pulmonary senescence-associated low-grade inflammation (SALI). Our studies indicate a natural ageing-associated molecular mechanism of telomerase deficiency-induced telomere DDR and SALI in pulmonary ageing and IPF.

Description: Background: The invention of non-ionizing emission devices revolutionized science, medicine, industry, and the military. Currently, different laser systems are commonly used, generating the potential threat of excessive radiation exposure, which can lead to adverse health effects. Skin is the organ most exposed to laser irradiation; therefore, this study aims to evaluate the effects of 445 nm, 520 nm, and 638 nm non-ionizing irradiation on keratinocytes and fibroblasts. Methods: Keratinocytes and fibroblasts were exposed to a different fluency of 445 nm, 520 nm, and 638 nm laser irradiation. In addition, viability, type of cell death, cell cycle distribution, and proliferation rates were investigated. Results: The 445 nm irradiation was cytotoxic to BJ-5ta (≥58.7 J/cm2) but not to Ker-CT cells. Exposure influenced the cell cycle distribution of Ker-CT (≥61.2 J/cm2) and BJ-5ta (≥27.6 J/cm2) cells, as well as the Bj-5ta proliferation rate (≥50.5 J/cm2). The 520 nm irradiation was cytotoxic to BJ-5ta (≥468.4 J/cm2) and Ker-CT (≥385.7 J/cm2) cells. Cell cycle distribution (≥27.6 J/cm2) of Ker-CT cells was also affected. The 638 nm irradiation was cytotoxic to BJ-5ta and Ker-CT cells (≥151.5 J/cm2). The proliferation rate and cell cycle distribution of BJ-5ta (≥192.9 J/cm2) and Ker-CT (13.8 and 41.3 J/cm2) cells were also affected. Conclusions: At high fluences, 455 nm, 520 nm, and 638 nm irradiation, representing blue, green, and red light spectra, are hazardous to keratinocytes and fibroblasts. However, laser irradiation may benefit the cells at low fluences by modulating the cell cycle and proliferation rate.

Description: Neurodegenerative diseases are a group of pathologies that cause severe disability due to motor and cognitive limitations. In particular, cognitive impairment is a growing health and socioeconomic problem which is still difficult to deal with today. As there are no pharmacologically effective treatments for cognitive deficits, scientific interest is growing regarding the possible impacts of healthy lifestyles on them. In this context, physical activity is gaining more and more evidence as a primary prevention intervention, a nonpharmacological therapy and a rehabilitation tool for improving cognitive functions in neurodegenerative diseases. In this descriptive overview we highlight the neurobiological effects of physical exercise, which is able to promote neuroplasticity and neuroprotection by acting at the cytokine and hormonal level, and the consequent positive clinical effects on patients suffering from cognitive impairment.

Description: Along with the cells that are exposed to radiation, non-irradiated cells can unveil radiation effects as a result of intercellular communication, which are collectively defined as radiation induced bystander effects (RIBE). Exosome-mediated signalling is one of the core mechanisms responsible for multidirectional communication of tumor cells and their associated microenvironment, which may result in enhancement of malignant tumor phenotypes. Recent studies show that exosomes and exosome-mediated signalling also play a dynamic role in RIBE in cancer cell lines, many of which focused on altered exosome cargo or their effects on DNA damage. However, there is a lack of knowledge regarding how these changes in exosome cargo are reflected in other functional characteristics of cancer cells from the aspects of invasiveness and metastasis. Therefore, in the current study, we aimed to investigate exosome-mediated bystander effects of 2 Gy X-ray therapeutic dose of ionizing radiation on the invasive potential of MCF-7 breast cancer cells in vitro via assessing Matrigel invasion potential, epithelial mesenchymal transition (EMT) characteristics and the extent of glycosylation, as well as underlying plausible molecular mechanisms. The findings show that exosomes derived from irradiated MCF-7 cells enhance invasiveness of bystander MCF-7 cells, possibly through altered miRNA and protein content carried in exosomes.

Description: There is tremendous demand for marine environmental observation, which requires the development of a multi-agent cooperative observation algorithm to guide Unmanned Surface Vehicles (USVs) and Unmanned Aerial Vehicles (UAVs) to observe isotherm data of the mesoscale vortex. The task include two steps: firstly, USVs search out the isotherm, navigate independently along the isotherm, and collect marine data; secondly, a UAV takes off, and in its one round trip, the UAV and USVs jointly perform the task of the UAV reading the observation data from USVs. In this paper, aiming at the first problem of the USV following the isotherm in an unknown environment, a data-driven Deep Deterministic Policy Gradient (DDPG) control algorithm is designed that allows USVs to navigate independently along isotherms in unknown environments. In addition, a hybrid cooperative control algorithm based on a multi-agent DDPG is adopted to solve the second problem, which enables USVs and a UAV to complete data reading tasks with the shortest flight distance of the UAV. The experimental simulation results show that the trained system can complete this tas, with good stability and accuracy.

Description: The objective of this study is to encapsulate flavonoids from yellow onion skins in whey protein isolates (WPI) and xylose (X), by Maillard-based conjugates, as an approach to improve the ability to entrap flavonoids and to develop powders with enhanced antioxidant activity. WPI (0.6%, w/v) was conjugated to X (0.3%, w/v) through the Maillard reaction at 90 °C for 120 min, in the presence of a flavonoid-enriched extract. Two variants of powders were obtained by freeze-drying. The glycation of WPI allowed a better encapsulation efficiency, up to 90.53 ± 0.29%, corresponding to a grafting degree of 30.38 ± 1.55%. The molecular modelling approach was used to assess the impact of X interactions with α-lactalbumin and β-lactoglobulin on the ability of these proteins to bind the main flavonoids from the yellow onion skins. The results showed that X might compete with quercetin glucosides to bind with α-lactalbumin. No interference was found in the case of β-lactoglobulin. The microstructural appearance of the powders revealed finer spherosomes in powder with WPI–X conjugates via the Maillard reaction. The powders were added to nachos, followed by a phytochemical characterization, in order to test their potential added value. An increase in antioxidant activity was observed, with no significant changes during storage.

Description: Muscle fatigue (MF) declines the capacity of muscles to complete a task over time at a constant load. MF is usually short-lasting, reversible, and is experienced as a feeling of tiredness or lack of energy. The leading causes of short-lasting fatigue are related to overtraining, undertraining/deconditioning, or physical injury. Conversely, MF can be persistent and more serious when associated with pathological states or following chronic exposure to certain medication or toxic composites. In conjunction with chronic fatigue, the muscle feels floppy, and the force generated by muscles is always low, causing the individual to feel frail constantly. The leading cause underpinning the development of chronic fatigue is related to muscle wasting mediated by aging, immobilization, insulin resistance (through high-fat dietary intake or pharmacologically mediated Peroxisome Proliferator-Activated Receptor (PPAR) agonism), diseases associated with systemic inflammation (arthritis, sepsis, infections, trauma, cardiovascular and respiratory disorders (heart failure, chronic obstructive pulmonary disease (COPD))), chronic kidney failure, muscle dystrophies, muscle myopathies, multiple sclerosis, and, more recently, coronavirus disease 2019 (COVID-19). The primary outcome of displaying chronic muscle fatigue is a poor quality of life. This type of fatigue represents a significant daily challenge for those affected and for the national health authorities through the financial burden attached to patient support. Although the origin of chronic fatigue is multifactorial, the MF in illness conditions is intrinsically linked to the occurrence of muscle loss. The sequence of events leading to chronic fatigue can be schematically denoted as: trigger (genetic or pathological) -〉 molecular outcome within the muscle cell -〉 muscle wasting -〉 loss of muscle function -〉 occurrence of chronic muscle fatigue. The present review will only highlight and discuss current knowledge on the molecular mechanisms that contribute to the upregulation of muscle wasting, thereby helping us understand how we could prevent or treat this debilitating condition.

Description: Effective gas dispersion and liquid mixing are significant parameters in the design of an inert-particle spouted-bed reactor (IPSBR) system. Solid particles can be used to ensure good mixing and an efficient rate of mass and heat transfer between the gas and liquid. In this study, computational fluid dynamics (CFD) coupled with the discrete phase model (DPM) were developed to investigate the effect of the feed gas velocity (0.5–1.5 m/s), orifice diameter (0.001–0.005 m), gas head (0.15–0.35 m), particle diameter (0.009–0.0225 m), and mixing-particle-to-reactor-volume fraction (2.0–10.0 vol.%) on the solid mass concentration, average solid velocity, and average solid volume fraction in the upper, middle, and conical regions of the reactor. Statistical analysis was performed using a second-order response surface methodology (RSM) with central composite design (CCD) to obtain the optimal operating conditions. Selected parameters were optimized to maximize the responses in the middle and upper regions, and minimize them in the conical region. Such conditions produced a high interfacial area and fewer dead zones owing to good particle dispersion. The optimal process variables were feed gas velocity of 1.5 m/s, orifice diameter of 0.001 m, gas head of 0.2025 m, a particle diameter of 0.01 m, and a particle load of 0.02 kg. The minimum average air velocity and maximum air volume fraction were observed under the same operating conditions. This confirmed the novelty of the reactor, which could work at a high feed gas velocity while maintaining a high residence time and gas volume fraction.

Description: In China, ground-level ozone has shown an increasing trend and has become a serious ambient pollutant. An accurate spatiotemporal distribution of ground-level ozone concentrations (GOCs) is urgently needed. Generalized linear models (GLMs) and Bayesian maximum entropy (BME) models are practical for predicting GOCs. However, GLMs have limited capacity to capture temporal variations and can miss some short-term and regional patterns, while the performance of BME models may degrade in cases of sparse or imperfect monitoring networks. Thus, to predict nationwide 1 km monthly average GOCs for China, we designed a novel hybrid model containing three modules. (1) A GLM was established to accurately describe the variability in GOCs in the space domain. (2) A BME model incorporating GLM residuals was employed to capture the temporal variability of GOCs in detail. (3) A combination of GLM and BME models was developed based on the specific broad range of each submodel. According to the cross-validation results, the hybrid model exhibited superior performance, with coefficient of determination (R2) values of 0.67. The predictive performance of the large-scale and high-resolution hybrid model is superior to that in previous studies. The nationwide spatiotemporal variability of the GOCs derived from the hybrid model shows that they are valuable indicators for ground-level ozone pollution control and prevention in China.

Description: Ammonia and phosphate, which are present in large quantities in waste streams such as livestock manure, are key compounds in fertilization activities. Their recovery will help close natural cycles and take a step forward in the framework of a circular economy. In this work, a lab-scale three-chambered microbial electrolysis cell (MEC) has been operated in continuous mode for the recovery of ammonia and phosphate from digested pig slurry in order to obtain a nutrient concentrated solution as a potential source of fertilizer (struvite). The maximum average removal efficiencies for ammonium and phosphate were 20% ± 4% and 36% ± 10%, respectively. The pH of the recovered solution was below 7, avoiding salt precipitation in the reactor. According to Visual MINTEQ software modelling, an increase of pH value to 8 outside the reactor would be enough to recover most of the potential struvite (0.21 mmol L−1 d−1), while the addition of up to 0.2 mM of magnesium to the nutrient recovered solution would enhance struvite production from 5.6 to 17.7 mM. The application of three-chambered MECs to the recovery of nutrients from high strength wastewater is a promising technology to avoid ammonia production through industrial processes or phosphate mineral extraction and close nutrient natural cycles.

Description: Voltage-dependent anion channels (VDACs) are the most important proteins in mitochondria. They localize to the outer mitochondrial membrane and contribute to the metabolite transport between the mitochondria and cytoplasm, which aids plant growth regulation. Here, we report that Arabidopsis thaliana VDAC1 is involved in the floral transition, with the loss of AtVDAC1 function, resulting in an early-flowering phenotype. AtVDAC1 is expressed ubiquitously in Arabidopsis. To identify the flowering pathway integrators that may be responsible for AtVDAC1′s function during the floral transition, an RNA-seq analysis was performed. In total, 106 differentially expressed genes (DEGs) were identified between wild-type and atvdac1-5 mutant seedlings. However, none were involved in flowering-related pathways. In contrast, AtVDAC1 physically associated with FLOWERING LOCUS T. Thus, in the floral transition, AtVDAC1 may function partly through the FLOWERING LOCUS T protein.

Description: The carbon dioxide (CO2) methanation reaction is a process that produces methane (CH4) by reacting CO2 and H2. Many studies have been conducted on this process because it enables a reduction of greenhouse gases and the production of energy with carbon neutrality. Moreover, it also exhibits a higher efficiency at low temperatures due to its thermodynamic characteristics; thus, there have been many studies, particularly on the catalysts that are driven at low temperatures and have high durability. However, with regards to employing this process in actual industrial processes, studies on both toxic substances that can influence catalyst performance and regeneration are still insufficient. Therefore, in this paper, the activity of a Ni catalyst before and after hydrogen sulfide (H2S) exposure was compared and an in-depth analysis was conducted to reveal the activity performance through the regeneration treatment of the poisoned catalyst. This study observed the reaction activity changes when injecting H2S during the CO2 + H2 reaction to evaluate the toxic effect of H2S on the Ni-Ce-Zr catalyst, in which the results indicate that the reaction activity decreases rapidly at 220 °C. Next, this study also successfully conducted a regeneration of the Ni-Ce-Zr catalyst that was poisoned with H2S by applying H2 heat treatment. It is expected that the results of this study can be used as fundamental data in an alternative approach to performance recovery when a small amount of H2S is included in the reaction gas of industrial processes (landfill gas, fire extinguishing tank gas, etc.) that can be linked to CO2 methanation.

Description: Antibody-drug conjugates (ADCs) are innovative biopharmaceutical products in which a monoclonal antibody is linked to a small molecule drug with a stable linker. Most of the ADCs developed so far are for treating cancer, but there is enormous potential for using ADCs to treat other diseases. Currently, ten ADCs have been approved by the United States Food and Drug Administration (FDA), and more than 90 ADCs are under worldwide clinical development. Monoclonal antibodies have evolved from research tools to powerful therapeutics in the past 30 years. Tremendous strides have been made in antibody discovery, protein bioengineering, formulation, and delivery devices. This manuscript provides an overview of the biology, chemistry, and biophysical properties of each component of ADC design. This review summarizes the advances and challenges in the field to date, with an emphasis on antibody conjugation, linker-payload chemistry, novel payload classes, drug-antibody ratio (DAR), and product development. The review emphasizes the lessons learned in the development of oncology antibody conjugates and look towards future innovations enabling other therapeutic indications. The review discusses resistance mechanisms to ADCs, and give an opinion on future perspectives.

Description: Wave energy has great prospect among many forms of marine renewable energy for its high density and storage. This paper proposes an underwater direct drive wave energy converter (UDDWEC), which is composed of a submerged point absorbing buoy and a linear-rotating axial flux permanent magnetic generator (LR-AFPMG). In addition, a maximum energy capture control strategy, resonance control, is derived for UDDWEC, based on small amplitude oscillation and hydrodynamic analysis. The proposed control strategy assumes the availability of sea condition such as wave height and period. This control strategy has three main characteristics. Firstly, this control strategy is derived based on hydrodynamic analysis of the submerged point absorber. Added mass, radiation damping and other hydrodynamic parameters are obtained to participate in UDDWEC dynamic model. Secondly, a LR-AFPMG is applied as power take-off device to realize energy conversion, which can improve the power density. Thirdly, small amplitude oscillation can be changed into long stroke rotary motion through the LR-AFPMG. The reliability and effectiveness of the proposed control strategy are assessed at various operation conditions for a heaving system and the validity for the UDDWEC is verified.

Description: Calmodulin (CaM), as an important factor in the calcium signaling pathway, is widely involved in plant growth and development regulation and responses to external stimuli. In this study, the full-length sequence of the ScCaM gene (GenBank: GQ246454) was isolated from the leaves of a Saccharum spp. hybrid. Prokaryotic expression showed that ScCaM could be solubly expressed and purified in Escherichia coli BL21. Subcellular localization confirmed that ScCaM was localized in the plasma membrane and nucleus of cells. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that ScCaM can be induced by various stresses, including sodium chloride (NaCl), chromium trichloride (CrCl3), salicylic acid (SA), and methyl jasmonate (MeJA). Ectopic expression in Arabidopsis thaliana demonstrated that ScCaM can affect the growth and development of transgenic plants. Moreover, the qRT-PCR analysis indicated that the overexpression of the allogenic ScCaM gene inhibits the expression of AtSTM, leading to the phenomenon of multiple-tillering in transgenic A. thaliana. The present study provided valuable information and facilitates further investigation into the function of ScCaM in the future.

Description: The aim of this work is to investigate niche variations in endemic Silene velutina (Caryophyllaceae, Angiosperms) on Mediterranean islands that differ in size. Six populations on both large and small islands were sampled across the geographic range of the species. For each population, 10 plots (1 × 2 m, with a 25 cm grill) were randomly placed to quantify environmental (abiotic and biotic factors and disturbance) and population (demographic structure and reproductive success) parameters. Niche parameters related to substrate, plant cover, community diversity and composition and disturbance showed significant variation in relation to island size. At the regional scale, we detected a broader niche on large islands associated with spatial heterogeneity and island size. In contrast, at the local scale, populations on small islands showed a broader niche, potentially due to a release from competition (low diversity and plant cover and absence of phanerophytes). Populations on large islands had a demographic structure biased towards vegetative individuals (seedlings and juveniles) with few reproductive individuals, while those on small islands had a majority of adults. Together, the results on niche breadth and demographic structure concord with the idea of a strategy based on adult persistence on small islands.

Description: Hot pepper (Capsicum annum L.) is a major spice crop and is used worldwide for its nutritional value. In the field, its plant is susceptible to various fungal diseases, including fusarium wilt, caused by soil-borne fungus Fusarium oxysporum f. sp. capsici, which can survive in the soil for several years. The infected plant can be recognized by the yellowing of older leaves and downward curling of apical shoots, followed by plant wilting and ultimately the death of the plant. The resistance mechanism in plants is controlled by a single dominant gene, and conventional plant breeding techniques are used to develop a wilt-resistant germplasm. Non-conventional techniques such as gene pyramiding and expression enhancement of antifungal genes could be used to shorten the time to develop resistance against fusarium wilt in hot peppers. In this review, we discuss different aspects of the disease and the molecular basis of resistance in chili/hot pepper plants. Furthermore, this review covers the scope of conventional and non-conventional breeding strategies and different management approaches used to tackle the disease.

Description: The Boa constrictor is one of the most common reptiles bred in captivity. To achieve a successful breeding season, thorough knowledge of the females’ reproductive activity is necessary. In this regard, information on the Boa constrictor is still rather scarce. The aim of the present study was to monitor the ovarian activity and the embryonic development of boas by ultrasound. We performed brief scans on thirty non-anaesthetized snakes using a portable ultrasound system and a 7.5–10 MHz linear array transducer (Esaote MyLab™ Classic). Ultrasound features, dimensions, and echogenicity of the preovulatory and postovulatory follicles were determined. As gestation progresses, the postovulatory follicle size increases, and the embryonic silhouette becomes increasingly recognizable. During the second month after ovulation, by using color Doppler, early embryos’ heart activity could be evaluated. It is possible to highlight vascular connections between the mother and the membrane covering the embryonic structures. Ultrasound also allows one to identify follicular regression or slugs (nonfertilized eggs) early. The present study suggests that ultrasound could be an excellent noninvasive technique to evaluate the reproductive activity of Boa constrictor, allowing us to precisely identify the correct time for mating, monitor embryo development and viability, and allow the early diagnosis of follicular regression.

Description: Arterial media calcification (AMC) is predominantly regulated by vascular smooth muscle cells (VSMCs), which transdifferentiate into pro-calcifying cells. In contrast, there is little evidence for endothelial cells playing a role in the disease. The current study investigates cellular functioning and molecular pathways underlying AMC, respectively by, an ex vivo isometric organ bath set-up to explore the interaction between VSMCs and ECs and quantitative proteomics followed by functional pathway interpretation. AMC development, which was induced in mice by dietary warfarin administration, was proved by positive Von Kossa staining and a significantly increased calcium content in the aorta compared to that of control mice. The ex vivo organ bath set-up showed calcified aortic segments to be significantly more sensitive to phenylephrine induced contraction, compared to control segments. This, together with the fact that calcified segments as compared to control segments, showed a significantly smaller contraction in the absence of extracellular calcium, argues for a reduced basal NO production in the calcified segments. Moreover, proteomic data revealed a reduced eNOS activation to be part of the vascular calcification process. In summary, this study identifies a poor endothelial function, next to classic pro-calcifying stimuli, as a possible initiator of arterial calcification.

Description: The nature of genome organization into two basic structural compartments is as yet undiscovered. However, it has been indicated to be a mechanism of gene expression regulation. Using the classification approach, we ranked genomic marks that hint at compartmentalization. We considered a broad range of marks, including GC content, histone modifications, DNA binding proteins, open chromatin, transcription and genome regulatory segmentation in GM12878 cells. Genomic marks were defined over CTCF or RNAPII loops, which are basic elements of genome 3D structure, and over 100 kb genomic windows. Experiments were carried out to empirically assess the whole set of features, as well as the individual features in classification of loops/windows, into compartment A or B. Using Monte Carlo Feature Selection and Analysis of Variance, we constructed a ranking of feature importance for classification. The best simple indicator of compartmentalization is DNase-seq open chromatin measurement for CTCF loops, H3K4me1 for RNAPII loops and H3K79me2 for genomic windows. Among DNA binding proteins, this is RUNX3 transcription factor for loops and RNAPII for genomic windows. Chromatin state prediction methods that indicate active elements like promoters, enhancers or heterochromatin enhance the prediction of loop segregation into compartments. However, H3K9me3, H4K20me1, H3K27me3 histone modifications and GC content poorly indicate compartments.

Description: The manipulation of light intensity by shade nets can lead to exchanges in the phytochemical quality and antioxidants ofsome herbs. This study aimed to determine whether shading by pearl nets (50% shade index) could improve the bioactive compounds in several medicinal herbs such as thyme (Thymus vulgaris L.), marjoram (Origanum majorana L.), oregano (Origanum vulgare L.), lemon balm (Melissa officinalis L.), and peppermint (Mentha piperita L.), and their correspondingherbal extracts during a four-week maceration process in ethanol. Oregano and thyme provided the highest yield of total extractive substances (TES) from both shaded and non-shaded plants. Among all studied herbs, the highest level of antioxidants, expressed as total phenolic content (TPC) was found in extracts from shaded plants of lemon balm. Herbal extracts produced from non-shaded thyme and marjoram had higher flavonoid contents compared to herbal extracts from shaded plants. Accumulation of the investigated secondary metabolites depends more on the specificity of a plant species rather than light intensity. This study was an initial step in the production of medicinal plants with an increased quantity of antioxidant and other bioactive compounds during the maceration process in extracts, aimed to be used as natural alcoholic productwith added value.

Description: Disordered eating, or abnormal eating behaviours that do not meet the criteria for an independent eating disorder, have been reported among people with schizophrenia. We aimed to systemati-cally review literature on disordered eating among people with schizophrenia spectrum disorder (SSD). Seven databases were systematically searched for studies that described the prevalence and correlates of disordered eating among patients with SSD from January 1984 to 15 February 2021. Qualitative analysis was performed using the National Institutes of Health scales. Of 5504 records identified, 31 studies involving 471,159 subjects were included in the systematic review. The ma-jority of studies (17) rated fair on qualitative analysis and included more men, and participants in their 30s and 40s, on antipsychotics. The commonest limitations include lack of sample size or power calculations, poor sample description, not using valid tools, or not adjusting for con-founders. The reported rates were 4.4% to 45% for binge eating, 16.1% to 64%, for food craving, 27% to 60.6% for food addiction, and 4% to 30% for night eating. Positive associations were re-ported for binge eating with antipsychotic use and female gender, between food craving and weight gain, between food addiction and increased dietary intake, and between disordered eating and female gender, mood and psychotic symptoms. Reported rates for disordered eating among people with SSD are higher than those in the general population. We will discuss the clinical, treatment and research implications of our findings.

Description: Rapid commercialisation of nano-enabled products (NEPs) elevates the potential environmental release of engineered nanomaterials (ENMs) along the product life cycle. The current review examined the state of the art literature on aquatic environment exposure and ecotoxicity of product released (PR) engineered nanomaterials (PR–ENMs). Additionally, the data obtained were applied to estimate the risk posed by PR–ENMs to various trophic levels of aquatic biota as a means of identifying priority NEPs cases that may require attention with regards to examining environmental implications. Overall, the PR–ENMs are predominantly associated with the matrix of the respective NEPs, a factor that often hinders proper isolation of nano-driven toxicity effects. Nevertheless, some studies have attributed the toxicity basis of observed adverse effects to a combination of the released ions, ENMs and other components of NEPs. Notwithstanding the limitation of current ecotoxicology data limitations, the risk estimated herein points to an elevated risk towards fish arising from fabrics’ PR–nAg, and the considerable potential effects from sunscreens’ PR–nZnO and PR–nTiO2 to algae, echinoderms, and crustaceans (PR–nZnO), whereas PR–nTiO2 poses no significant risk to echinoderms. Considering that the current data limitations will not be overcome immediately, we recommend the careful application of similar risk estimation to isolate/prioritise cases of NEPs for detailed characterisation of ENMs’ release and effects in aquatic environments.

Description: Cancer is one of the leading causes of death globally, associated with multifactorial pathophysiological components. In particular, genetic mutations, infection or inflammation, unhealthy eating habits, exposition to radiation, work stress, and/or intake of toxins have been found to contribute to the development and progression of cancer disease states. Early detection of cancer and proper treatment have been found to enhance the chances of survival and healing, but the side effects of anticancer drugs still produce detrimental responses that counteract the benefits of treatment in terms of hospitalization and survival. Recently, several natural bioactive compounds were found to possess anticancer properties, capable of killing transformed or cancerous cells without being toxic to their normal counterparts. This effect occurs when natural products are associated with conventional treatments, thereby suggesting that nutraceutical supplementation may contribute to successful anticancer therapy. This review aims to discuss the current literature on four natural bioactive extracts mostly characterized by a specific polyphenolic profile. In particular, several activities have been reported to contribute to nutraceutical support in anticancer treatment: (1) inhibition of cell proliferation, (2) antioxidant activity, and (3) anti-inflammatory activity. On the other hand, owing to their attenuation of the toxic effect of current anticancer therapies, natural antioxidants may contribute to improving the compliance of patients undergoing anticancer treatment. Thus, nutraceutical supplementation, along with current anticancer drug treatment, may be considered for better responses and compliance in patients with cancer. It should be noted, however, that when data from studies with bioactive plant preparations are discussed, it is appropriate to ensure that experiments have been conducted in accordance with accepted pharmacological research practices so as not to disclose information that is only partially correct.

Description: The cross-flow rotating packed bed (RPB) has attracted wide attention in recent years because of its advantages of large gas capacity, low pressure drop and lack of flooding limitation. However, the complex structure of the packing makes it difficult to obtain the gas flow characteristics in the cross-flow RPB by experiments. In this study, the dry pressure drop in the cross-flow RPB was investigated by computational fluid dynamics (CFD). The packing was modeled by the porous media model and the rotation of the packing was simulated by the sliding mesh model. The simulation results obtained by three turbulence models were compared with experimental results, and the RNG k-ε model was found to best describe the turbulence behaviors in the cross-flow RPB. Then, the effects of gas flow rate and rotating speed on dry pressure drop in different parts of the cross-flow RPB were analyzed. The results of this study can provide important insights into the design and scale-up of cross-flow RPB.

Description: We consider alternative possibilities for hedging spot positions on the FTSE LATIBEX Index, the index of the only international market exclusively for Latin American firms that is denominated by the euro. Since there is not a futures market on the index, it is unclear whether a relatively successful hedge can be found. We explore the plausibility of employing futures on four stock market indices: EUROSTOXX 50, S&P500, BOVESPA, and IPC, and simulate the results that could be obtained by a hedge position based on either unconditional or conditional second order moments estimated from different asymmetric GARCH models. Several criteria for hedging effectiveness suggest that futures contracts on BOVESPA should be preferred, and that a salient reduction in risk can be achieved over the unhedged LATIBEX portfolio. The evidence in favor of a better performance of conditional moments is very clear, without significant differences among the alternative GARCH specifications.

Description: Cadmium (Cd) is one of the most injurious heavy metals, affecting plant growth and development. Melatonin (N-acetyl-5-methoxytryptamine) was discovered in plants in 1995, and it is since known to act as a multifunctional molecule to alleviate abiotic and biotic stresses, especially Cd stress. Endogenously triggered or exogenously applied melatonin re-establishes the redox homeostasis by the improvement of the antioxidant defense system. It can also affect the Cd transportation and sequestration by regulating the transcripts of genes related to the major metal transport system, as well as the increase in glutathione (GSH) and phytochelatins (PCs). Melatonin activates several downstream signals, such as nitric oxide (NO), hydrogen peroxide (H2O2), and salicylic acid (SA), which are required for plant Cd tolerance. Similar to the physiological functions of NO, hydrogen sulfide (H2S) is also involved in the abiotic stress-related processes in plants. Moreover, exogenous melatonin induces H2S generation in plants under salinity or heat stress. However, the involvement of H2S action in melatonin-induced Cd tolerance is still largely unknown. In this review, we summarize the progresses in various physiological and molecular mechanisms regulated by melatonin in plants under Cd stress. The complex interactions between melatonin and H2S in acquisition of Cd stress tolerance are also discussed.

Description: This study aimed to investigate the effects of multi-bacteria solid-state fermented diets with different crude fiber (CF) levels on growth performance, nutrient digestibility, and microbial flora of finishing pigs. The multi-bacteria solid-state fermented diets were made up of Lactobacillus amylovorus, Enterococcus faecalis, Bacillus subtilis, and Candida utilis. According to a 2 (factors) × 2 (levels) design, with the two factors being multi-bacteria solid-state fermentation (fed non-fermented diet or multi-bacteria fermentation) or CF levels (fed a basal diet containing 2.52% CF or 7.00% CF), a total of 36 finishing pigs (70.80 ± 5.75 kg) were divided into 4 treatments with 9 barrows per group: (1) pigs fed a diet containing 7.00% CF (HF), (2) pigs fed a multi-bacteria fermentation diet containing 7.00% CF (HFM), (3) pigs fed a diet containing 2.52% CF (LF), and (4) piglets fed a multi-bacteria fermentation diet containing 2.52% CF (LFM). This experiment lasted 28 days. The multi-bacteria solid-state fermented diet increased the backfat thickness (p 〈 0.05) and apparent total tract nutrient digestibility (ATTD) of CF, neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), 8 amino acids (Trp, Asp, Gly, Cys, Val, Met, Ile, and Leu), total essential amino acids (EAA), total non-essential amino acids (NEEA), and total amino acids (TAA) (p 〈 0.05). Multi-bacteria solid-state fermented diet increased serum concentrations of HDL-c, ABL, TP, and GLU, the serum enzyme activities of GSH-Px, T-AOC, SOD, and CAT (p 〈 0.05), the relative abundance of Lactobacillus, Oscillospira, and Coprococcus (p 〈 0.05), and the abundance of YAMINSYN3-PWY, PWY-7013, GOLPDLCAT-PWY, ARGORNPROST-PWY, and PWY-5022 pathways (p 〈 0.05). The multi-bacteria solid-state fermented diet reduced the digestion amount of CF, NDF, and ADF (p 〈 0.05), the serum concentrations of TC, TG, LDL-c, BUN, and MDA (p 〈 0.05), the relative abundance of Streptococcaceae (p 〈 0.05), and the abundance of PWY-6470, PWY0-862, HSERMETANA-PWY, LACTOSECAT-PWY, MET-SAM-PWY, PWY-6700, PWY-5347, PWY0-1061, and LACTOSECAT-PWY pathways (p 〈 0.05). The high-fiber diet increased average daily feed intake (p 〈 0.05), the serum concentrations of TC, TG, LDL-c, BUN, and MDA (p 〈 0.05), the relative abundance of Clostridiaceae_Clostridium and Coprococcus (p 〈 0.05), and the abundance of TCA-GLYOX-BYPASS, GLYCOLYSIS-TCA-GLYOX-BYPASS, and PWY-6906 pathways (p 〈 0.05). The high-fiber diet reduced chest circumference (p 〈 0.05) and ATTD of ether extract (EE), CF, NDF, ADF, Ca, CP, 18 amino acids (Trp, Thr, Val, Met, Ile, Leu, Phe, Lys, His, Arg Asp, Ser, Glu, Gly, Ala, Cys, Tyr, and Pro), EAA, NEAA, and TAA (p 〈 0.05). The high-fiber diet also reduced the serum concentrations of HDL-c, TP, ABL, and GLU, the serum enzyme activities of T-AOC, GSH-Px, SOD, and CAT (p 〈 0.05), and the relative abundance of Akkermansia and Oscillospira (p 〈 0.05). There was no significant effect of the interaction between multi-bacteria fermentation and dietary CF levels, except on the digestion amount of CF (p 〈 0.05). The 7.00% CF had a negative effect on the digestion of nutrients, but multi-bacteria solid-state fermentation diets could relieve this negative effect and increase backfat thickness. High-fiber diets and multi-bacteria solid-state fermentation improved the diversity and abundance of fecal microorganisms in finishing pigs.

Description: In the first quarter of 2020, Italy became one of the earliest hotspots of COVID-19 infection, and the government imposed a lockdown. During the lockdown, an online survey of 2053 adults was conducted that asked about health behaviors and about the psychological and overall impact of COVID-19. The present study is a secondary analysis of that data. We hypothesized that self-control, higher socio-economic status, existing health conditions, and fear of infection were all inversely related to actions (or intentions) that violated the lockdown (i.e., infractions). Using partial least squares structural equation modeling (PLS-SEM), we found that only the fear of infection significantly dissuaded people from violating lockdown rules. Since it is not practical or ethical to sow a fear of infection, our study indicates that enacting rules and enforcing them firmly and fairly are important tools for containing the infection. This may become more important as vaccines become more widely available and people lose their fear of infection.

Description: This paper summarizes the results from microclimatic monitoring of the impacts related to the construction and exploration phase of a hydroelectric powerplant in the upper Douro River (Portugal). Reference climatic elements for both periods were obtained and the impacts of the reservoir water mass on the region’s microclimate were evaluated. For this purpose, Sen’s slope estimate non-parametric test was used to detect the meteorological variables’ trends over 10 years, considering the division into the construction (2011–2015) and exploration (2016–2020) phases. A network of meteorological stations located close to the dam, in addition to a control station relatively distant from the dam, was used to collect the meteorological data. The control station is still integrated into the same regional climatic context but not exposed to the direct influence of the resulting reservoirs. As a result, temporal differences were determined for temperature and relative humidity. The results show a statistically significant increase in the minimum relative humidity, accompanied by a statistically significant decrease in the daily and seasonal temperature amplitudes between the construction and the exploration phase periods. These outcomes thereby suggest that large reservoirs affect the local climate and may create new microclimates, in the surrounding area, with both positive and negative potential effects.

Description: In Europe, the qualification of injection anchors in masonry under static and quasi-static actions is based on an assessment of tests performed in undamaged masonry. Nevertheless, in seismic prone countries like Italy the influences deriving from earthquake actions cannot be disregarded. Masonry elements are very sensitive to cyclic/seismic action and research on the behavior of anchors in damaged masonry is rather limited. The paper presents the results of an experimental campaign aimed at evaluating the residual tensile strength of adhesive anchors installed into undamaged walls that were subsequently subjected to cyclic in-plane loading to simulate seismic actions before. Consequently, the anchors experienced different stresses depending on their location within the walls. Overall, 29 tests were performed with anchors placed both, in undamaged and damaged areas. The results showed that there is a correlation between residual tensile strength and masonry initial conditions, and therefore the installation of anchors in masonry elements should be carefully planned avoiding areas that could be heavily damaged during seismic events or considering redundant connections in critical areas. In particular, it seems that the width of the crack (created by cyclic actions) that passes nearby/into the anchor borehole is the main parameter that affects the ultimate resistance of the anchors.

Description: Despite a keen interest in clinical research, most paramedical professionals are unwilling to play an active role. Our objective was to explore paramedical professionals’ representations of research. Using an existing database of final year paramedical students (speech therapy, occupational therapy, psychomotricity, audiometry, physiotherapy, orthoptics), we deployed a qualitative approach composed of two successive steps: (1) a free word association task, and (2) semi-structured individual interviews. Out of the 54 students who agreed to be contacted, we received 21 responses to the free word association questionnaire, and 11 interviews were conducted. The hierarchical evocation matrix revealed that the scientific representation of research is based on words defining the research and the purpose of the research. “Collaboration” was identified as being an essential part of the research process. The central core of the representation is coherent with all its components perceived as positive. The content analysis of the interviews showed a polarization around two key points: (1) participants are interested in accessing and using evidence in their practice (2) but feel less confident about and/or motivated to generate evidence themselves. This study highlights the need to develop more research-friendly environments, especially in training institutions.

Description: The United Nations 2030 Agenda has emphasized the potential of digital technology to enhance sustainability performance, assuming that digital transformation can enable firms’ convergence toward the Sustainable Development Goals. Despite this, the literature is unclear regarding whether there is a positive relationship between digitalization and sustainability, as the effects of digital transformation are controversial. The main goal of this study was to assess the hypothesis that digital technology contributes to the achievement of Sustainable Development Goals within the UN 2030 Agenda. To test this hypothesis, a textual analysis was performed to assess Italian firms’ digitalization efforts; the obtained results were then related to the selected firms’ ESG scores using a regression analysis. The analysis focused on Italian FTSE MIB listed firms for the period 2016–2019. The findings show a positive relation between digitalization and Sustainable Development Goals, highlighting the relevance of digital technology in implementing the sustainability agenda.

Description: Optimization and sensitivity analysis of the kinetic parameters of the catalytic ozonation process is crucial to improve water treatment, reactor design, and construction. This study evaluated the optimization of the kinetic constants for Diclofenac (DCF) degradation during catalytic ozonation with Goethite (FeOOH, as a catalyst) through different kinetic modeling approaches. A central composite design was used to evaluate the effect of ozone dose and catalyst loading. The results showed that FeOOH did not significantly influence the degradation of DCF, while the reactivity of DCF with ozone was high (with 〉90% degradations in 20 min). However, the variation in catalyst loading significantly affected TOC removal (〉10%) and ozone use, with ozone efficiency in ozone transfer (RU) 5% higher than ozonation. After evaluating the different kinetic models of reaction speed by optimizing kinetic parameters and performing sensitivity analysis for the treatment of DCF by catalytic ozonation, it can be concluded that the addition of FeOOH improved the kinetics of the decomposition of ozone and the yield in the production of hydroxyl radicals.

Description: We consider the predictor-corrector numerical methods for solving Caputo–Hadamard fractional differential equations with the graded meshes logtj=loga+logtNajNr,j=0,1,2,…,N with a≥1 and r≥1, where loga=logt0