ALBERT

Description: Along the Norwegian coasts and margins, extensive reefs of the stony coral Lophelia pertusa act as hotspots for local biodiversity. Climate models project that the temperature of Atlantic deep waters could rise by 1–3°C by 2100. In this context, understanding the effects of temperature on the physiology of cold-water species will help in evaluating their resilience to future oceanic changes. We investigated the response of L. pertusa to stepwise short-term increases in temperature. We sampled corals from four reefs, two located north of the Arctic circle and two at the mid-Norwegian shelf (boreal). In on-board experiments (one per reef), the sampled fragments were exposed to increasing temperatures from 5 to 15°C over 58 h. Respiration increased linearly by threefold for a 10°C increase. The short-term temperature increase did not induce mortality, cellular (neutral red assay for lysosome membrane stability; but one exception) or oxidative stress (lipid peroxidation assay) – to a few exceptions. However, the variability of the respiration responses depended on the experiment (i.e., reef location), possibly linked to the genetic structure of the individuals that we sampled (e.g., clones or siblings). The corals from the Arctic and boreal regions appear to have a high tolerance to the rapid temperature fluctuations they experience in the field. Over extended periods of time however, an increased metabolism could deplete the energy stored by the corals, if not met by an increased food availability and/or uptake. Empirical data on organisms’ thermal performance curves, such as the one presented in this study for L. pertusa, will be useful to implement predictive models on the responses of species and populations to climate change.

Description: Vertebrates are recognized as sentient beings. Consequently, urgent priority is now being given to understanding the needs and maximizing the welfare of animals under human care. The general health of animals is most commonly determined by physiological indices e.g., blood sampling, but may also be assessed by documenting behavior. Physiological health assessments, although powerful, may be stressful for animals, time-consuming and costly, while assessments of behavior can also be time-consuming, subject to bias and suffer from a poorly defined link between behavior and health. However, behavior is recognized as having the potential to code for stress and well-being and could, therefore, be used as an indicator of health, particularly if the process of quantifying behavior could be objective, formalized and streamlined to be time efficient. This study used Daily Diaries (DDs) (motion-sensitive tags containing tri-axial accelerometers and magnetometers), to examine aspects of the behavior of bycaught loggerhead turtles, Caretta caretta in various states of health. Although sample size limited statistical analysis, significant behavioral differences (in terms of activity level and turn rate) were found between “healthy” turtles and those with external injuries to the flippers and carapace. Furthermore, data visualization (spherical plots) clearly showed atypical orientation behavior in individuals suffering gas emboli and intestinal gas, without complex data analysis. Consequently, we propose that the use of motion-sensitive tags could aid diagnosis and inform follow-up treatment, thus facilitating the rehabilitation process. This is particularly relevant given the numerous rehabilitation programs for bycatch sea turtles in operation. In time, tag-derived behavioral biomarkers, TDBBs for health could be established for other species with more complex behavioral repertoires such as cetaceans and pinnipeds which also require rehabilitation and release. Furthermore, motion-sensitive data from animals under human care and wild conspecifics could be compared in order to define a set of objective behavioral states (including activity levels) for numerous species housed in zoos and aquaria and/or wild species to help maximize their welfare.

Description: Marine food webs form the major component of the biological pump and play a central role in the global carbon (C) cycle. Understanding the response of particular processes in marine food webs to changing environments is a prerequisite to predict changes in ecological functioning in the future ocean. Here, we experimentally assessed the effects of nitrogen:phosphorus (N:P) supply ratios (the molar ratios 10:1, 24:1, and 63:1) on elemental and biochemical quality of marine phytoplankton Rhodomonas sp., and the interactions between food quantity and quality on stoichiometric C:N:P, fatty acids (FAs) and reproductions in copepods Acartia tonsa. Overall, the stoichiometry of A. tonsa was to some extent homeostatic in response to the changing algal C:N and C:P ratios, with significant changes in C:N ratios of A. tonsa observed, especially under higher food quantities. The relative gross growth efficiencies (GGEs) for C and N (and P) were analyzed, revealing that copepods may achieve homeostasis by lowering the GGE for C while increasing it for the limiting nutrient. Egg production rates in A. tonsa were lowest on nutrient deficient diets under low food quantities. Reduced egg production rates may be attributed to the lowered GGEs for C and reduced transfer efficiency of essential FAs between phytoplankton and copepods, indicating interactive-essential effects of elements and FAs on copepod production. Our results highlight that nutrient deficiency in the environments may reduce energy transfer efficiency at the base of food webs by altering phytoplankton chemical composition, which can interact with food quantity and have implications on food web dynamics in the changing ocean.

Description: In the autumn of 2014, nine large mesocosms were deployed in the oligotrophic subtropical North-Atlantic coastal waters off Gran Canaria (Spain). Their deployment was designed to address the acidification effects of CO2 levels from 400 to 1,400 mu atm, on a plankton community experiencing upwelling of nutrient-rich deep water. Among other parameters, chlorophyll a (chl-a), potential respiration (Phi), and biomass in terms of particulate protein (B) were measured in the microplankton community (0.7-50.0 mu m) during an oligotrophic phase (Phase I), a phytoplankton-bloom phase (Phase II), and a post-bloom phase (Phase III). Here, we explore the use of the Phi/chl-a ratio in monitoring shifts in the microplankton community composition and its metabolism. Phi/chl-a values below 2.5 mu L O-2 h(-1) (mu g chl-a)(-1) indicated a community dominated by photoautotrophs. When Phi/chl-a ranged higher, between 2.5 and 7.0 mu L O-2 h(-1) (pg chl-a)(-1) , it indicated a mixed community of phytoplankton, microzooplankton and heterotrophic prokaryotes. When Phi/chl-a rose above 7.0 mu L O-2 h(-1) (mu g chl-a)(-1), it indicated a community where microzooplankton proliferated (〉10.0 mu L O-2 h(-1) (mu g chl-a)(-1)), because heterotrophic dinoflagellates bloomed. The first derivative of B, as a function of time (dB/dt), indicates the rate of protein build-up when positive and the rate of protein loss, when negative. It revealed that the maximum increase in particulate protein (biomass) occurred between 1 and 2 days before the chl-a peak. A day after this peak, the trough revealed the maximum net biomass loss. This analysis did not detect significant changes in particulate protein, neither in Phase I nor in Phase III. Integral analysis of Phi/chl-a and B, over the duration of each phase, for each mesocosm, reflected a positive relationship between 4) and pCO(2) during Phase II [alpha = 230.10-5 mu L O-2 h(-1) L-1 (patm CO2)(-1) (phase-day)(-1), R-2 = 0.30] and between chl-a and pCO(2) during Phase III [alpha = 100.10(-5) Ag chl-a L-1 (mu atmCO(2))(-1) (phase-day)(-1), R-2 = 0.84]. At the end of Phase II, a harmful algal species (HAS), Vicicitus globosus, bloomed in the high pCO(2) mesocosms. In these mesocosms, microzooplankton did not proliferate, and chl-a retention time in the water column increased. In these V globosus-disrupted communities, the (Phi/chl-a ratio [4.1 +/- 1.5 /mu L O-2 h(-1) (mu g chl-a)(-1)] was more similar to the Phi/chl-a ratio in a mixed plankton community than to a photoautotroph-dominated one.

Description: Due to ocean acidification and global warming, surface seawater of the western Baltic Sea is expected to reach an average of ∼1100 μatm pCO2 and an increase of ∼5°C by the year 2100. In four consecutive experiments (spanning 10–11 weeks each) in all seasons within 1 year, the abiotic factors temperature (+5°C above in situ) and pCO2 (adjusted to ∼1100 μatm) were tested for their single and combined effects on epibacterial communities of the brown macroalga Fucus vesiculosus and on bacteria present in the surrounding seawater. The experiments were set up in three biological replicates using the Kiel Outdoor Benthocosm facility (Kiel, Germany). Phylogenetic analyses of the respective microbiota were performed by bacterial 16S (V1-V2) rDNA Illumina MiSeq amplicon sequencing after 0, 4, 8, and 10/11 weeks per season. The results demonstrate (I) that the bacterial community composition varied in time and (II) that relationships between operational taxonomic units (OTUs) within an OTU association network were mainly governed by the habitat. (III) Neither single pCO2 nor pCO2:Temperature interaction effects were statistically significant. However, significant impact of ocean warming was detected varying among seasons. (IV) An indicator OTU (iOTU) analysis identified several iOTUs that were strongly influenced by temperature in spring, summer, and winter. In the warming treatments of these three seasons, we observed decreasing numbers of bacteria that are commonly associated with a healthy marine microbial community and—particularly during spring and summer—an increase in potentially pathogenic and bacteria related to intensified microfouling. This might lead to severe consequences for the F. vesiculosus holobiont finally affecting the marine ecosystem.

Description: In this paper, we investigate the seasonal and spatial variability of stratification on the Siberian shelves with a case study from the Laptev Sea based on shipboard hydrographic measurements, year-round oceanographic mooring records from 2013 to 2014 and chemical tracer-based water mass analyses. In summer 2013, weak onshore-directed winds caused spreading of riverine waters throughout much of the eastern and central shelf. In contrast, strong southerly winds in summer 2014 diverted much of the freshwater to the northeast, which resulted in 50% less river water and significantly weaker stratification on the central shelf compared with the previous year. Our year-long records additionally emphasize the regional differences in water column structure and stratification, where the northwest location was well-mixed for 6 months and the central and northeast locations remained stratified into spring due to the lower initial surface salinities of the river-influenced water. A 26 year record of ocean reanalysis highlights the region’s interannual variability of stratification and its dependence on winds and sea ice. Prior the mid-2000s, river runoff to the perennially ice-covered central Laptev Sea shelf experienced little surface forcing and river water was maintained on the shelf. The transition toward less summer sea ice after the mid-2000s increased the ROFI’s (region of freshwater influence) exposure to summer winds. This greatly enhanced the variability in mixed layer depth, resulting in several years with well-mixed water columns as opposed to the often year-round shallow mixed layers before. The extent of the Lena River plume is critical for the region since it modulates nutrient fluxes and primary production, and further controls intermediate heat storage induced by lateral density gradients, which has implications for autumnal freeze-up and the eastern Arctic sea ice volume. MAIN POINTS 1. CTD surveys and moorings highlight the regional and temporal variations in water column stratification on the Laptev Sea shelf. 2. Summer winds increasingly control the extent of the region of freshwater influence under decreasing sea ice. 3. Further reductions in sea ice increases surface warming, heat storage, and the interannual variability in mixed layer depth.

Description: Groundwater resources in coastal regions are facing enormous pressure caused by population growth and climate change. Few studies have investigated whether offshore freshened groundwater systems are connected with terrestrial aquifers recharged by meteoric water, or paleo-groundwater systems that are no longer associated with terrestrial aquifers. Distinguishing between the two has important implications for potential extraction to alleviate water stress for many coastal communities, yet very little is known about these connections, mainly because it is difficult to acquire continuous subsurface information across the coastal transition zone. This study presents a first attempt to bridge this gap by combining three complementary near-surface electromagnetic methods to image groundwater pathways within braided alluvial gravels along the Canterbury coast, South Island, New Zealand. We show that collocated electromagnetic induction, ground penetrating radar, and transient electromagnetic measurements, which are sensitive to electrical contrasts between fresh (low conductivity) and saline (high conductivity) groundwater, adequately characterize hydrogeologic variations beneath a mixed sand gravel beach in close proximity to the Ashburton River mouth. The combined measurements – providing information at three different depths of investigation and resolution – show several conductive zones that are correlated with spatial variations in subsurface hydrogeology. We interpret the conductive zones as high permeability conduits corresponding to lenses of well-sorted gravels and secondary channel fill deposits within the braided river deposit architecture. The geophysical surveys provide the basis for a discharge model that fits our observations, namely that there is evidence of a multilayered system focusing groundwater flow through stacked high permeability gravel layers analogous to a subterranean river network. Coincident geophysical surveys in a region further offshore indicate the presence of a large, newly discovered freshened groundwater system, suggesting that the offshore system in the Canterbury Bight is connected with the terrestrial aquifer system.

Description: The GO-SHIP nutrient manual covers all aspects of nutrient analysis from basic sample collection and storage, specifically for Continuous Flow analysis using an Auto-Analyzer, and describes some specific nutrient methods for Nitrate, Nitrite, Silicate, Phosphate and Ammonium that are in use by many laboratories carrying out at-sea analysis and repeat hydrography sections across the world. The focus is on segmented flow analyzers not flow injection analyzers. It also covers laboratory best practices including quality control and quality assurance (QC/QA) procedures to obtain the best results, and suggests protocols for the use of reference materials (RM) and certified reference materials (CRMs).

Description: Chromophoric dissolved organic matter (CDOM) is the dominant absorber of ultraviolet radiation in the ocean, but its sources within the ocean, as well as its chemical composition, remain uncertain. One source of marine CDOM is Sargassum, an epipelagic marine macro brown alga common to the Gulf of Mexico, Caribbean, and Western North Atlantic. Furthermore, Sargassum contains phlorotannins, a class of polyphenols that may have similar optical properties to terrestrial polyphenols. Here, we analyze Sargassum CDOM optical properties, acquired from absorption and fluorescence spectra of filtered samples collected during Sargassum exudation experiments in seawater tanks. To further evaluate the structural basis of Sargassum CDOM optical properties, Sargassum CDOM was collected by solid phase extraction (SPE) and its chemical composition was tested by pH titration and sodium borohydride reduction. These chemical tests revealed that Sargassum CDOM absorption spectra respond similarly to pH titration and borohydride reduction when compared to terrestrially-derived materials, but Sargassum CDOM has unique absorbance peaks in difference spectra that have not been observed in terrestrially-derived CDOM. These absorbance features are consistent with the deprotonation of modified Sargassum phlorotannins, which are likely highly related phenolic acids and polyphenols. Sargassum CDOM was also more rapidly photodegraded when compared to terrestrial CDOM such as Suwannee River Natural Organic Matter. Similar to terrestrial DOM, ultrahigh resolution mass spectrometry revealed that sunlight decreases relative abundances of m/z ions and molecular formulas with an average O/C ratio of 0.6 and an average H/C ratio of 0.9, suggesting preferential photodegradation and/or phototransformation of hydrogen-deficient and oxygenated compounds, such as Sargassum phlorotannins. Assuming a large fraction of Sargassum CDOM is quickly mineralized to CO2 during its rapid photodegradation, Sargassum could play a major role in marine photochemical carbon mineralization during its annual growth cycle