ALBERT

Description: Thermoterraces in syngenetic ice complexes are widespread along the erosion dominated Yakutia Arctic coast. Thermoterraces progressively record quantitative information about their existence, which may be used to determine the mean shore retreat rate during the time they are present. Initial measurements of four thermoterraces on the south coast of the Dmitry Laptev Strait were carried out by the authors in 2002 and shore retreat rates were calculated. Comparison of erosion rates obtained using thermoterrace dimensions and geodetic survey results with those determined using aerial photographs showed that erosion rate values obtained in these two ways are approximately of the same order.

Description: A multidisciplinary analysis of intraplate volcanic complexes interbedded with shallow and deeper marine sediments of a Late Miocene carbonate platform (Iblean Plateau, Sicily) has allowed a detailed paleo-environmental reconstruction. Our approach includes sedimentology, physical volcanology, stratigraphy, geochemistry/mineralogy, paleontology and 40Ar/39Ar dating. Four volcanic complexes are distinguished from each other. Two comprise an eastern shallow water platform (diatreme field and Carlentini complex) and two a western deeper water environment representing a seamount belt on the carbonate ramp (Valle Guffari seamount and Mineo complex). The late Miocene volcanism was not time-equivalent: episodic eruptions took place from the Late Tortonian (ca. 9.38 Ma at Mt. Carrubba) to Early Messinian (ca. 6.46 Ma at Valle Guffari). Explosive volcanism of the diatreme field may be related geodynamically to the period of periodic sea-level oscillations at the onset of the Messinian Salinity Crisis. Marine diatomites preserved in the crater areas of two diatremes are the only remnants of Early Messinian diatomites in the eastern Iblean Mountains.

Description: Meltponds on Arctic sea ice have previously been reported to be devoid of marine metazoans due to fresh-water conditions. The predominantly dark frequently also green and brownish meltponds observed in the Central Arctic in summer 2007 hinted to brackish conditions and considerable amounts of algae, possibly making the habitat suitable for marine metazoans. Environmental conditions in meltponds as well as sympagic meiofauna in new ice covering pond surfaces and in rotten ice on the bottom of ponds were studied, applying modified techniques from sea-ice and under-ice research. Due to the very porous structure of the rotten ice, the meltponds were usually brackish to saline, providing living conditions very similar to sub-ice water. The new ice cover on the surface had similar characteristics as the bottom layer of level ice. The ponds were thus accessible to and inhabitable by metazoans. The new ice cover and the rotten ice were inhabited by various sympagic meiofauna taxa, predominantly ciliates, rotifers, acoels, nematodes and foraminiferans. Also, sympagic amphipods were found on the bottom of meltponds. We suggest that, in consequence of global warming, brackish and saline meltponds are becoming more frequent in the Arctic, providing a new habitat to marine metazoans.

Description: Prey regurgitation during capture is a potential important confounding effect in fish dietary ecology studies as it may lead to overestimation of stomach vacuity and underestimation of prey consumption. This study investigates patterns of prey regurgitation and stomach vacuity among five grouper and three snapper species in shallow water off French Polynesia and tests the effectiveness of piercing swim-bladders after capture as a method to prevent regurgitation. Groupers exhibited a moderate overall regurgitation rate of 15.6% of full stomachs and a high true (i.e., after accounting for regurgitation) stomach vacuity rate of 40.5%. In contrast, snappers showed high regurgitation (mean 31.7%) and low true stomach vacuity (14.6%). Not accounting for regurgitation would have resulted in a moderate overestimation of stomach vacuity in groupers, but an almost 3-fold overestimation in snappers. Swim-bladder decompression by piercing after capture prove a highly effective method to reduce regurgitation (more than 2-fold for groupers and near 8-fold for snappers). This study enables a more general understanding of prey regurgitation in two commercially valuable fish families, thus improving understanding of the dietary ecology of these fishes. This information is particularly important in the context of prey consumption estimates and subsequent estimations of the impact of fish predators on ecosystems.

Description: The temporal variation in the stable carbon and nitrogen isotope ratios of particulate organic matter (POM) in the Rhone River was investigated on a monthly basis during a 2-year period (2004–2005). In spite of high month-to-month variation, interannually consistent seasonal trends appeared, with significantly lower δ13C (〈−28.2‰) in spring than in the other seasons. In contrast, δ15N did not exhibit significant temporal variation. In spring and summer, high chlorophyll a and b concentrations were associated with low C/N values (〈8) and a high percentage of organic carbon (%C) and organic nitrogen (%N), testifying to high development of autochthonous riverine phytoplankton (mainly diatoms and chlorophytes). In fall and winter, higher δ13C (〉−27.2‰) and C/N (〉8) values, and lower %C, %N, and chlorophylls concentrations indicated the predominance of allochthonous terrestrial detritus material in the river POM. The lower δ13C values recorded in spring–summer, when the phytoplankton biomass was high, were related to the lower carbon isotopic signatures of freshwater diatoms and chlorophytes compared to those of terrestrial plants. Overall, Rhone River POM was mainly composed of terrestrially derived material (90%), with autochthonous phytoplankton representing only 10% as a mean, in spite of a higher mean contribution of phytoplankton (27%) to river POM in summer.

Description: Nutritional imbalances between predator and prey are the rule rather than the exception at the lower end of food webs. We investigated the role of different grazers in the propagation of nutritionally imbalanced primary production by using the same primary producers in a three-trophic-level food chain and a four-trophic-level food chain experimental setup. The three-trophic-level food chain consisted of a classic single-cell primary producer (Rhodomonas salina), a metazoan grazer (the copepod Acartia tonsa) and a top predator (the jellyfish Gonionemus vertens), while we added a protozoan grazer (Oxyrrhis marina) as primary consumer to the food chain to establish the four-trophic-level food chain. This setup allowed us to investigate how nutrient-limitation effects change from one trophic level to another, and to investigate the performance of two components of our experimental food chains in different trophic positions. Stoichiometry and fatty acid profiles of the algae showed significant differences between the nutrient-depleted [no N and no P addition (−P), respectively] and the nutrient-replete (f/2) treatments. The differences in stoichiometry could be traced when O. marina was the first consumer. Copepods feeding on these flagellates were not affected by the nutritional imbalance of their prey in their stoichiometry, their respiration rates nor in their developmental rates. In contrast, when copepods were the primary consumer, those reared on the −P algae showed significantly higher respiration rates along with significantly lower developmental rates. In neither of our two experimental food chains did the signals from the base of the food chains travel up to jelly fish, our top predator.

Description: A one-dimensional model is used to analyze, at the local scale, the response of the equatorial Atlantic Ocean under different meteorological conditions. The study was performed at the location of three moored buoys of the Pilot Research Moored Array in the Tropical Atlantic located at 10° W, 0° N; 10° W, 6° S; and 10° W, 10° S. During the EGEE-3 (Etude de la circulation océanique et de sa variabilité dans le Golfe de Guinee) campaign of May-June 2006, each buoy was visited for maintenance during 2 days. On board the ship, high-resolution atmospheric parameters were collected, as were profiles of temperature, salinity, and current. These data are used here to initialize, force, and validate a one-dimensional model in order to study the diurnal oceanic mixed-layer variability. It is shown that the diurnal variability of the sea surface temperatures is mainly driven by the solar heat flux. The diurnal response of the near-surface temperatures to daytime heating and nighttime cooling has an amplitude of a few tenths of degree. The computed diurnal heat budget experiences a net warming tendency of 31 and 27 W m‑2 at 0° N and 10° S, respectively, and a cooling tendency of 122 W m‑2 at 6° S. Both observed and simulated mixed-layer depths experience a jump between the nighttime convection phase and the well-stabilized diurnal water column. Its amplitude changes dramatically depending on the meteorological conditions occurring at the stations and reaches its maximum amplitude (~50 m) at 10° S. At 6° and 10° S, the presence of barrier layers is observed, a feature that is clearer at 10° S. Simulated turbulent kinetic energy (TKE) dissipation rates, compared to independent microstructure measurements, show that the model tracks their diurnal evolution reasonably well. It is also shown that the shear and buoyancy productions and the vertical diffusion of TKE all contribute to the supply of TKE, but the buoyancy production is the main source of TKE during the period of the simulation.

Description: Background, aim, and scope Dissolved humic substances (HSs) are exogenous stressors to aquatic plants and animals which activate a variety of transcriptional and biochemical reactions or block photosynthesis. While there are consistent indications which structures may lead to reduced photosynthetic activity, there is much less clear information available on which HS structures or building blocks act as stressors in animals. Consequently, this work was designed to comparatively study the impact of natural organic matter (NOMs) from different sources on major anti-stress mechanisms in one single animal. We utilized major antioxidant responses and relative expression levels of stress proteins (small HSPs and HSP70) and expected that different HSs provoke different response patterns. Materials and methods We tested the freshwater amphipod Gammarus pulex which was collected from several shallow creeks in Northern Germany. All specimens were maintained in aerated 5-L tanks with filtered water from their natural environment at 10°C with prior acclimation. Animals were fed ad libitum with a commercial preparation once every second day. The exposure water was exchanged with the same frequency. NOMs were isolated from three different sources: two from small brown-water lakes in Northern Germany by reverse osmosis and the third one as an aqueous extract from a black layer of a Brazilian sandbar soil (State of Rio de Janeiro). The rationale was to apply NOMs of contrasting quality. Chemical fingerprint features of the NOMs were taken by high-performance size exclusion chromatography. As stress parameters in the animals, the activities of peroxidase and catalase were recorded quantitatively, and stress proteins, HSP70, as well as small α-crystalline HSPs were analyzed semiquantitatively. Results The three NOMs clearly differed in molecular masses, humic substance contents, the moieties of polysaccharides, and low-molecular-weight substances. With the exception of one short-term response, the peroxidase activity increased after 3 to 12 h exposure, whereas the catalase activity did not show any significant modulation. With one exception, the stress protein expression increased after 30 min exposure in a biphasic pattern, and the sHSPs responded less strongly than HSP70. Discussion Although the quality of the exposed NOMs differs significantly, a rather uniform response pattern appears in the animals. Obviously, the contrasting contents of HSs and polysaccharides did not affect the anti-stress response of the exposed gammarids which is in contrast to previous lifespan studies with Caenorhabditis elegans. Furthermore, all NOM sources led to increased contents of both HSP70 and sHSPs. To the best of our knowledge, this is the first protein study to show that also small HSPs are expressed when the animals are exposed against humic material. Conclusions Since the response patterns of the exposed gammarids, in contrast to the initial hypothesis, are rather uniform and since HSs are parts of life on Earth, we furthermore presume that they may have been a primordial exogenous trigger for the development of anti-stress systems in exposed organisms. Recommendations and perspectives Effect studies of chemical stresses on organisms should consider exposure to both natural triggers and xenobiotic compounds in low concentrations—in order to prospectively differentiate between these triggers and, subsequently, classify them.

Description: The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody α (H-300) raised against the human α1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol gFM−1 h−1) than in those of L. vulgaris (31.8 ± 3.3 µmol gFM−1 h−1). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmolATP gFM−1 h−1, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.

Description: The phytoplankton of the Bahía Blanca Estuary, Argentina, has been surveyed since 1978. Chlorophyll a, phytoplankton abundance, species composition and physico-chemical variables have been fortnightly recorded. From 1978 to 2002, a single winter–early spring diatom bloom has dominated the main pattern of phytoplankton interannual variability. Such pattern showed noticeable changes since 2006: the absence of the typical winter bloom and changes in phenology, together with the replacement of the dominant blooming species, i.e. Thalassiosira curviseriata, and the appearance of different blooming species, i.e. Cyclotella sp. and Thalassiosira minima. The new pattern showed relatively short-lived diatom blooms that spread throughout the year. In addition, shifts in the phytoplankton size structure toward small-sized diatoms, including the replacement of relatively large Thalassiosira spp. by small Cyclotella species and Chaetoceros species have been noticed. The changes in the phenology and composition of the phytoplankton are mainly attributed to warmer winters and the extremely dry weather conditions evidenced in recent years in the Bahía Blanca area. Changing climate has modified the hydrological features in the inner part of the estuary (i.e. higher temperatures and salinities) and potentially triggered the reorganization of the phytoplankton community. This long-term study provides evidence on species-specific and structural changes at the bottom of the pelagic food web likely related to the recent hydroclimatic conditions in a temperature estuary of the southwestern Atlantic.

Description: Somatic mutations are an underappreciated source of genetic variation within multi-cellular organisms. The resulting genetic mosaicism should be particularly abundant in large clones of vegetatively propagating angiosperms. Little is known on the abundance and ecological correlates of genetic mosaicism in field populations, despite its potential evolutionary significance. Because sexual reproduction restores genetic homogeneity, we predicted that in facultatively clonally reproducing organisms, the prevalence of genetic mosaicism increases with increasing clonality. This was tested among 33 coastal locations colonized by the ecologically important marine angiosperm Zostera marina, ranging from Portugal to Finland. Genetic mosaics were detectable as complex microsatellite genotypes at two hypervariable loci that revealed additional mosaic alleles, suggesting the presence of one or more divergent cell lineages within the same ramet. The proportions of non-mosaic genotypes in a population sharply decreased below a clonal richness of 0.2. Accordingly, more genetic mosaics were found at the southern and northern limit of the distribution of Z. marina in Europe where sexual reproduction is rare or absent. The genetic mosaics observed at neutral microsatellite markers suggest the possibility of within-clone variation at selectively relevant loci and supports the notion that members of clones are seldom genetically identical.

Description: A new species of the pelagic marine copepod family Oncaeidae, Oncaea serrulata, is described from the Mediterranean Sea. The species belongs to the notopus-group of Oncaeidae, which are characterized by a long, free exopod on leg 5. It differs from Oncaea notopus Giesbrecht 1891 and any other described species of this group by a combination of morphological characters, including: (1) the relative lengths of distal endopod spines on swimming legs 2 and 3, (2) the denticulate ornamentation of the exopod segment and the two exopodal setae on leg 5, (3) the proportional lengths of the caudal setae, and (4) the comparatively small body length. The species occurs widespread in the Mediterranean Sea in mesopelagic and deep-sea layers down to 3,000 m depth and was also found in near-bottom sediment traps moored at a bottom depth of 2,347 m in the Ligurian Sea. O. serrulata appears to be the only representative of the notopus-group in the Mediterranean Sea and seems to have consistently been confounded with the allegedly cosmopolitan O. notopus Giesbrecht in most earlier studies in this area. The taxonomic history of Giesbrecht’s O. notopus, which was originally described from the Pacific, is summarized and morphological identification parameters applicable for the species identification of notopus-type oncaeids are discussed. In the future, identification may be facilitated by using genetic barcodes in comparison with those recently analysed for the Mediterranean O. serrulata (as Oncaea sp. 7 sensu Böttger-Schnack 1997). Published data on the zoogeographical and vertical distribution as well as the abundance of notopus-type oncaeids in the Mediterranean Sea are reviewed. Possible causes for the lack of positive records of this taxon in Mediterranean studies earlier than 1980 are discussed.

Description: Regional erosion of the Rock Garden ridge top, a bathymetric high within New Zealand’s Hikurangi Subduction Margin, is likely associated with its gas hydrate system. Seismic data reveal gas pockets that appear partially trapped beneath the shallow base of gas hydrate stability. Steady-state fluid flow simulations, conducted on detailed two-dimensional geological models, reveal that anomalous fluid pressure can develop close to the sea floor in response to lower-permeability hydrate-bearing sediments and underlying gas pockets. Transient simulations indicate that large-scale cycling of fluid overpressure may occur on time scales of a few to tens of years. We predict intense regions of hydro-fracturing to preferentially develop beneath the ridge top rather than beneath the flanks, due to more pronounced overpressure generation and gas migration through hydrate-bearing sediments. Results suggest that sediment weakening and erosion of the ridge top by hydro-fracturing could be owed to fluid dynamics of the shallow gas hydrate system.

Description: Detailed geological and petrological-geochemical study of rocks of the lava complex of Young Shiveluch volcano made it possible to evaluate the lava volumes, the relative sequence in which the volcanic edifice was formed, and the minimum age of the onset of eruptive activity. The lavas of Young Shiveluch are predominantly magnesian andesites and basaltic andesites of a mildly potassic calc-alkaline series (SiO2 = 55.0–63.5 wt %, Mg# = 55.5–68.9). Geologic relations and data on the mineralogy and geochemistry of rocks composing the lava complex led us to conclude that the magnesian andesites of Young Shiveluch volcano are of hybrid genesis and are a mixture of silicic derivatives and a highly magnesian magma that was periodically replenished in the shallow-depth magmatic chamber. The fractional crystallization of plagioclase and hornblende at the incomplete segregation of plagioclase crystals from the fractionating magmas resulted in adakitic geochemical parameters (Sr/Y = 50–71, Y 〈 18 ppm) of the most evolved rock varieties. Our results explain the genesis of the rock series of Young Shiveluch volcano without invoking a model of the melting of the subducting Pacific slab at its edge.

Description: The construction of S-wave velocity models of marine sediments down to hundreds of meters below the seafloor is important in a number of disciplines. One of the most significant trends in marine geophysics is to use interface waves to estimate shallow shear velocities which play an important role in determining the shallow crustal structure. In marine settings, the waves trapped near the fluid-solid interface are called Scholte waves, and this is the subject of the study. In 1998, there were experiments on the Ninetyeast Ridge (Central Indian Ocean) to study the shallow seismic structure at the drilled site. The data were acquired by both ocean bottom seismometer and ocean bottom hydrophone. A new type of seafloor implosion sources has been used in this experiment, which successfully excited fast and high frequency (> 500 Hz) body waves and slow, intermediate frequency (〈 20 Hz) Scholte waves. The fundamental and first higher mode Scholte waves have both been excited by the implosion source. Here, the Scholte waves are investigated with a full waveform modeling and a group velocity inversion approach. Shear wave velocities for the uppermost layers of the region are inferred and results from the different methods are compared. We find that the full waveform modeling is important to understand the intrinsic attenuation of the Scholte waves between 1 and 20 Hz. The modeling shows that the S-wave velocity varies from 195 to 350 m/s in the first 16 m of the uppermost layer. Depths levels of high S-wave impedance contrasts compare well to the layer depth derived from a P-wave analysis as well as from drilling data. As expected, the P- to S-wave velocity ratio is very high in the uppermost 16 m of the seafloor and the Poisson ratio is nearly 0.5. Depth levels of high S-wave impedance contrasts are comparable to the layer depth derived from drilling data.

Description: We investigated the effect of commercially available enzymes (α-amylase, α-galactosidase, papain, trypsin, and lipase) as well as proteases from deep-sea bacteria on the larval attachment of the bryozoan Bugula neritina L. The 50% effective concentrations (EC50) of the commercial proteases were 10 times lower than those of other enzymes. Crude proteases from six deep-sea Pseudoalteromonas species significantly decreased larval attachment at concentrations of 0.03 to 1 mIU ml−1. The EC50 of the pure protease from the bacterium Pseudoalteromonas issachenkonii UST041101-043 was close to 1 ng ml−1 (0.1 mIU ml−1). The protease and trypsin individually incorporated in a water-soluble paint matrix inhibited biofouling in a field experiment. There are certain correlations between production of proteases by bacterial films and inhibition of larval attachment. None of the bacteria with biofilms that induced attachment of B. neritina produced proteolytic enzymes, whereas most of the bacteria that formed inhibitive biofilms produced proteases. Our investigation demonstrated the potential use of proteolytic enzymes for antifouling defense.

Description: The production of defence metabolites is assumed to be costly in metabolic terms. If this holds true, low-light stress should reduce the ability of seaweeds to defend themselves chemically against herbivory and fouling. We investigated the effect of energy limitation on the defensive status of seaweeds by assessing their attractiveness to mesograzers and their activity against a bivalve macrofouler in comparison with non-stressed conspecifics. The macroalgae Codium decorticatum (Woodw.) M. Howe, Osmundaria obtusiloba (C. Agardh) R. E. Norris, Pterocladiella capillacea (S. G. Gmel.) Santel. and Hommer., Sargassum vulgare C. Agardh and Stypopodium zonale (Lamour.) Papenf. collected at the southeastern Brazilian coast were exposed to six levels of irradiation (between 1 and 180 μmol photons m−2 s−1) for 10–14 days. After this period, algae from all treatment levels were: (a) processed as artificial food and offered to an amphipod community dominated by Elasmopus brasiliensis Dana and (b) extracted to test for differences in settlement rates of the fouling mussel Perna perna L. on filter paper loaded with the crude extracts. Generally, photosynthesis rates and growth were reduced under low light conditions. Attractiveness to herbivores and macrofoulers, however, was insensitive to energy limitation. We discuss possible explanations for the observed absence of a relationship between light availability and algal defence including the change in nutritional value of the algal tissue, the allocation of resources towards defence instead of growth and the absence of costs for defence.

Description: We have applied the novel analytical method NanoSIMS to cephalopod statoliths for the first time in order to analyse their chemical microstructure, using a spatial resolution of 400 nm. This technique makes it possible to analyse in situ nano-scale chemical variations between increment layers. In statoliths of the boreoatlantic armhook squid Gonatus fabricii, we found distinct concentration patterns indicating a periodicity in strontium and sodium distributions. Sr and Na show a negative relation, both elements showing alternating patterns where the increments vary in width between approximately 1 and 5 μm. Results suggest, that aragonite deposited during the night is rich in Na and poor in Sr, while aragonite deposited during the day is rich in Sr and poor in Na. This study demonstrates the excellent suitability of NanoSIMS for nano-scale microchemical analyses of aragonite, providing new information on calcification processes and individual life histories. Possible future fields of application include not only cephalopod statoliths, but also virtually all biomineralized tissues in aquatic organisms like fish otoliths, gastropod statoliths, bivalve shells, foraminifers and corals.

Description: An indoor mesocosm system was set up to study the response of phytoplankton and zooplankton spring succession to winter and spring warming of sea surface temperatures. The experimental temperature regimes consisted of the decadal average of the Kiel Bight, Baltic Sea, and three elevated regimes with 2°C, 4°C, and 6°C temperature difference from that at baseline. While the peak of the phytoplankton spring bloom was accelerated only weakly by increasing temperatures (1.4 days per degree Celsius), the subsequent biomass minimum of phytoplankton was accelerated more strongly (4.25 days per degree Celsius). Phytoplankton size structure showed a pronounced response to warming, with large phytoplankton being more dominant in the cooler mesocosms. The first seasonal ciliate peak was accelerated by 2.1 days per degree Celsius and the second one by 2.0 days per degree Celsius. The over-wintering copepod populations declined faster in the warmer mesocosm, and the appearance of nauplii was strongly accelerated by temperature (9.2 days per degree Celsius). The strong difference between the acceleration of the phytoplankton peak and the acceleration of the nauplii could be one of the “Achilles heels” of pelagic systems subject to climate change, because nauplii are the most starvation-sensitive life cycle stage of copepods and the most important food item of first-feeding fish larvae.

Description: Pore water profiles from 24 stations in the South Atlantic (located in the Guinea, Angola, Cape, Guyana, and Argentine basins) show good correlations of oxygen and silicon, suggesting microbially mediated dissolution of biogenic silica. We used simple analytical transport and reaction models to show the tight coupling of the reconstructed process kinetics of aerobic respiration and silicon regeneration. A generic transport and reaction model successfully reproduced the majority of Si pore water profiles from aerobic respiration rates, confirming that the dissolution of biogenic silica (BSi) occurs proportionally to O2 consumption. Possibly limited to well-oxygenated sediments poor in BSi, benthic Si fluxes can be inferred from O2 uptake with satisfactory accuracy. Compared to aerobic respiration kinetics, the solubility of BSi emerged as a less influential parameter for silicon regeneration. Understanding the role of bacteria for silicon regeneration requires further investigations, some of which are outlined. The proposed aerobic respiration control of benthic silicon cycling is suitable for benthic–pelagic models. The empirical relation of BSi dissolution to aerobic respiration can be used for regionalization assessments and estimates of the silicon budget to in crease the understanding of global primary and export production patterns.

Description: In 2000, the Carbon Dioxide in the Ocean working group of the North Pacific Marine Science Organization (PICES) performed an international experiment on the intercalibration of the measurements of the total alkalinity in seawater using certified reference materials (CRM). Taking part in this experiment, Russian specialists presented the method by Bruevich. The results of the intercalibration showed that the alkalinity values obtained by Bruevich's method using modern burettes, an Na2CO3 reactant of high purity as a standard to ascertain the acid titre, and corrections for the acid density and for the weights of the acid and seawater samples in vacuum are in agreement with the standard within +/- 1 mu M/kg.

Description: The pelagic marine copepod family Oncaeidae is highly diversified (over 100 species worldwide) and includes a great number of sibling species, which are difficult to identify morphologically, because of their very small size (0.18–1.2 mm total length as adults). Global investigations of oncaeid biodiversity are severely hampered by insufficient taxonomic knowledge, in particular for species which have first been described from the European Mediterranean Sea (type locality). Many of these species have been reported as key taxa of small-sized copepod communities in very distant oceanic regions. However, due to the taxonomic uncertainties it cannot be excluded that at least some of these allegedly cosmopolitan species in reality represent a complex of distinct, yet closely related, species. To improve the basis for the identification of Oncaeidae of Mediterranean origin, new diagnostic characters in combination with traditional methods were applied in the present study. Copepods were sampled with fine nets of 0.1 mm mesh size down to a maximum depth of 1,000 m on a west-east-transect in the Mediterranean Sea. Oncaeid species and form variants were predefined morphologically and the genetic identity of the morphospecies was analysed by about 650 and 500 bp region of the mitochondrial COI and 12S srRNA gene sequence, respectively (barcoding). A total of 67 individuals from 24 oncaeid species and forms were successfully analysed, including 12 species and one form of Mediterranean origin. For Oncaeidae, the 12S amplification turned out to be more successful (23 species) than the COI amplification (13 species and 1 form). Together, the morphological and molecular results are discussed with respect to three topics: (1) confirmation of a genetic distinction of three Triconia species, which have been interpreted as sibling species by morphological characters, (2) genetic distance of species within the ovalis-complex of oncaeids and (3) the taxonomic status of two form variants of Oncaea mediterranea (Claus).

Description: NOD-like receptors (NLRs) exert pivotal roles in innate immunity as sensors of exogenous or endogenous cellular danger signals. The NLR protein family has a characteristic domain architecture comprising a central nucleotide binding and oligomerization domain (NOD), an N-terminal effector binding domain and C-terminal leucine-rich repeats (LRRs). Mutations in NLR genes are genetically associated with a number of chronic inflammatory diseases of barrier organs. In this chapter, we focus on the influence of NLR regulation and function in the complex pathophysiology of mucosal homeostasis. The understanding of NLR biology may guide our future understanding of how the interaction between the human genome and the metagenome of transient and resident microbiota precipitates into chronic inflammatory disorders, such as Crohn's disease or atopy.

Description: Fabric and growth mode of deep-water isidid gorgonian skeletons showing bright Mg-calcitic internodes and dark proteinageous nodes were investigated on modern, subrecent and fossil skeletons. The internodial microstructure is characterised by three-dimensionally interfingering calcitic fascicles accreting around a central axis. Macroscopic colour banding results from varying orientations of organic-rich fascicle bundles and intercalated bands of organic-poor granular crystals. This skeletal structure of isidid gorgonians strikingly differs from the density banding of scleractinians. Radiocarbon dating of a fossil skeleton gave an age of 3,985±35 to 3,680±35 years before present (BP) with a record of 305±35 years (±range). Linear extension rates of 0.4 mm year−1 average allow for an annual to sub-annual resolution on micrometer scale of colour bands or fascicles, respectively. The growth mode of branched skeletons is characterised by simultaneous secretion of vertically alternating nodes/internodes and lateral accretion of concentric increments enveloping the entire skeleton. Bifurcations at various growth stages imply that adjacent branches have different ages and show varying numbers of growth bands at any skeletal cross section. The scleroprotein gorgonin plays a crucial role in the formation of organic nodes and the secretion of calcitic internodes by providing a structural framework in the biomineralisation process.

Description: Humans have interacted with the Wadden Sea since its origin 7,500 years ago. However, exploitation, habitat alteration and pollution have strongly increased since the Middle Ages, affecting abundance and distribution of many marine mammals, birds, fish, invertebrates and plants. Large whales and some large birds disappeared more than 500 years ago. Most small whales, seals, birds, large fish and oysters were severely reduced by the late 19th and early 20th centuries, leading to the collapse of several traditional fisheries. In the 20th century, conservation efforts have enabled some breeding birds and seals to recover. But other species declined further due to continuing exploitation, habitat destruction, pollution and eutrophication. Moreover, complex three-dimensional habitats such as oyster banks, Sabellaria reefs and subtidal eelgrass beds have been lost completely. In contrast, several opportunistic species such as gulls, polychaetes, green algae and exotic invaders increased during the 20th century. Taken together, multiple human impacts have caused dramatic losses of large predators and habitat-building species in the Wadden Sea over the last 500 years. Although still of high natural value and global importance, the Wadden Sea is a fundamentally changed ecosystem. On the other hand, reduced hunting pressure, increased habitat protection and reduced river pollution have enabled the recent recovery of several species and an increase in environmental quality. These successes, together with a historical vision of what was once possible, should guide current and future conservation, restoration and management efforts towards a more sustainable interaction between man and the sea.

Description: Todayrsquos Wadden Sea is a heavily human-altered ecosystem. Shaped by natural forces since its origin 7,500 years ago, humans gradually gained dominance in influencing ecosystem structure and functioning. Here, we reconstruct the timeline of human impacts and the history of ecological changes in the Wadden Sea. We then discuss the ecosystem and societal consequences of observed changes, and conclude with management implications. Human influences have intensified and multiplied over time. Large-scale habitat transformation over the last 1,000 years has eliminated diverse terrestrial, freshwater, brackish and marine habitats. Intensive exploitation of everything from oysters to whales has depleted most large predators and habitat-building species since medieval times. In the twentieth century, pollution, eutrophication, species invasions and, presumably, climate change have had marked impacts on the Wadden Sea flora and fauna. Yet habitat loss and overexploitation were the two main causes for the extinction or severe depletion of 144 species (~20% of total macrobiota). The loss of biodiversity, large predators, special habitats, filter and storage capacity, and degradation in water quality have led to a simplification and homogenisation of the food web structure and ecosystem functioning that has affected the Wadden Sea ecosystem and coastal societies alike. Recent conservation efforts have reversed some negative trends by enabling some birds and mammals to recover and by creating new economic options for society. The Wadden Sea history provides a unique long-term perspective on ecological change, new objectives for conservation, restoration and management, and an ecological baseline that allows us to envision a rich, productive and diverse Wadden Sea ecosystem and coastal society.

Description: During the twentieth century sea surface temperatures in the Atlantic Ocean exhibited prominent multidecadal variations. The source of such variations has yet to be rigorously established—but the question of their impact on climate can be investigated. Here we report on a set of multimodel experiments to examine the impact of patterns of warming in the North Atlantic, and cooling in the South Atlantic, derived from observations, that is characteristic of the positive phase of the Atlantic Multidecadal Oscillation (AMO). The experiments were carried out with six atmospheric General Circulation Models (including two versions of one model), and a major goal was to assess the extent to which key climate impacts are consistent between the different models. The major climate impacts are found over North and South America, with the strongest impacts over land found over the United States and northern parts of South America. These responses appear to be driven by a combination of an off-equatorial Gill response to diabatic heating over the Caribbean due to increased rainfall within the region and a Northward shift in the Inter Tropical Convergence Zone (ITCZ) due to the anomalous cross-equatorial SST gradient. The majority of the models show warmer US land temperatures and reduced Mean Sea Level Pressure during summer (JJA) in response to a warmer North Atlantic and a cooler South Atlantic, in line with observations. However the majority of models show no significant impact on US rainfall during summer. Over northern South America, all models show reduced rainfall in southern hemisphere winter (JJA), whilst in Summer (DJF) there is a generally an increase in rainfall. However, there is a large spread amongst the models in the magnitude of the rainfall anomalies over land. Away from the Americas, there are no consistent significant modelled responses. In particular there are no significant changes in the North Atlantic Oscillation (NAO) over the North Atlantic and Europe in Winter (DJF). Additionally, the observed Sahel drying signal in African rainfall is not seen in the modelled responses. Suggesting that, in contrast to some studies, the Atlantic Multidecadal Oscillation was not the primary driver of recent reductions in Sahel rainfall.

Description: The genes and intergenic regions of the amoCAB operon were analyzed to establish their potential as molecular markers for analyzing ammonia-oxidizing betaproteobacterial (beta-AOB) communities. Initially, sequence similarity for related taxa, evolutionary rates from linear regressions, and the presence of conserved and variable regions were analyzed for all available sequences of the complete amoCAB operon. The gene amoB showed the highest sequence variability of the three amo genes, suggesting that it might be a better molecular marker than the most frequently used amoA to resolve closely related AOB species. To test the suitability of using the amoCAB genes for community studies, a strategy involving nested PCR was employed. Primers to amplify the whole amoCAB operon and each individual gene were tested. The specificity of the products generated was analyzed by denaturing gradient gel electrophoresis, cloning, and sequencing. The fragments obtained showed different grades of sequence identity to amoCAB sequences in the GenBank database. The nested PCR approach provides a possibility to increase the sensitivity of detection of amo genes in samples with low abundance of AOB. It also allows the amplification of the almost complete amoA gene, with about 300 bp more sequence information than the previous approaches. The coupled study of all three amo genes and the intergenic spacer regions that are under different selection pressure might allow a more detailed analysis of the evolutionary processes, which are responsible for the differentiation of AOB communities in different habitats.

Description: We analyzed bare human footprints in Holocene tuff preserved in two pits in the Acahualinca barrio in the northern outskirts of Managua (Nicaragua). Lithology, volcanology, and age of the deposits are discussed in a companion paper (Schmincke et al. Bull Volcanol doi: 10.1007/s00445-008-0235-9, 2008). The footprint layer occurs within a series of rapidly accumulated basaltic–andesitic tephra that is regionally correlated to the Masaya Triple Layer Tephra. The people were probably trying to escape from a powerful volcanic eruption at Masaya Caldera 20 km farther south that occurred at 2.1 ka BP. We subdivided the swath of footprints, up to 5.6 m wide, in the northern pit (Pit I) into (1) a central group of footprints made by about six individuals, the total number being difficult to determine because people walked in each other’s footsteps one behind the other and (2) two marginal groups on either side of the central group with more widely spaced tracks. The western band comprises tracks of three adjacent individuals and an isolated single footprint farther out. The eastern marginal area comprises an inner band of deep footprints made by three individuals and, farther out, three clearly separated individuals. We estimate the total number of people as 15–16. In the southern narrow and smaller pit (Pit II), we recognize tracks of ca. 12 individuals, no doubt made by the same group. The group represented in both pits probably comprised male and female adults, teenagers and children based on differences in length of footprints and of strides and depth of footprints made in the soft wet ash. The smallest footprints (probably made by children) occur in the central group, where protection was most effective. The footprint layer is composed of a lower 5–15-cm thick, coarse-grained vesicle tuff capped by a medium to fine-grained tuff up to 3 cm thick. The surface on which the people walked was muddy, and the soft ash was squeezed up on the sides of the foot imprints and between toes. Especially, deep footprints are mainly due to local thickening of the water-rich ash, multiple track use, and differences in weight of individuals. The excellent preservation of the footprints, ubiquitous mudcracks, sharp and well-preserved squeeze-ups along the margins of the tracks and toe imprints, and the absence of raindrop impressions all suggest that the eruption occurred during the dry season. The people walked at a brisk pace, as judged from the tight orientation of the swath and the length of the strides. The directions of a major erosional channel in the overlying deposits that probably debouched into Lake Managua and the band of footprints are strictly parallel, indicating that people walked together in stride along the eastern margin of a channel straight toward the lake shore, possibly a site with huts and/or boats for protection and/or escape.

Description: We examined 323 cephalopods collected by bottom trawl from depths between 162 and 536 m near Disko island, West Greenland. The most abundant species were the boreoatlantic armhook squid Gonatus fabricii, the sepiolid Rossia moelleri and the spoonarm octopus Bathypolypus arcticus. From this sampling we provide new information on the population structure, bathymetric distribution and the reproductive biology of cephalopods from this area. Special emphasis is given to the sepiolid R. moelleri, for which the life cycle is proposed. Further, this study provides new relationships of beak size to mantle length and wet body mass for G. fabricii, R. moelleri and B. arcticus.

Description: Small cephalopods of the genus Heteroteuthis are the most pelagic members in the family Sepiolidae. This study examines the reproductive biology of Heteroteuthis dispar (Rüppell, 1844), the first such study on any member of the genus, based on 46 specimens (27 females and 19 males) collected during the Mar-Eco cruise in the North Atlantic in the region of the Mid-Atlantic Ridge in 2004, and compares it with reproductive features in the less pelagic members of the family. The unusually large spermatophores of the males have a very small ejaculatory apparatus and cement body, relative to the size of the sperm mass. Females first mate when they are still maturing: a large sperm mass (up to 3.4% of the female body mass), consisting of one to several spermatangia, was found in an internal seminal receptacle of the majority of the females examined regardless of their maturity state. The seminal receptacle has a unique form and position in this species. The receptacle is a thin-walled sac at the posterior end of the visceral mass that is an outpocketing of, and opens into, the visceropericardial coelom. Spermatangia and sperm from the spermatangia apparently enter into the visceropericardial coelom (which is mostly occupied by the ovary) from the seminal receptacle indicating that ova are fertilised internally, a strategy unknown for decapodiform cephalopods (squid and cuttlefish), but present in most octopods. Fecundity of Heteroteuthis dispar (1,100–1,300 oocytes) is much higher than in other sepiolids whereas the egg size (mean max. length ∼1.6 mm) is the smallest within the family. Spawning is continuous (sensu Rocha et al. in Biol Rev 76:291–304, 2001). These and other reproductive traits are discussed as being adaptations to an oceanic lifestyle.

Description: Stable isotope signatures of primary producers display high inter- and intraspecific variation. This is assigned to species-specific differences in isotope fractionation and variable abiotic conditions, e.g., temperature, and nutrient and light availability. As consumers reflect the isotopic signature of their food source, such variations have direct impacts on the ecological interpretation of stable isotope data. To elucidate the variability of isotope fractionation at the primary producer level and the transfer of the signal through food webs, we used a standardised marine tri-trophic system in which the primary producers were manipulated while the two consumer levels were kept constant. These manipulations were (1) different algal species grown under identical conditions to address interspecific variability and (2) a single algal species cultivated under different nutrient regimes to address nutrient-dependent variability. Our experiments resulted in strong interspecific variation between different algal species (Thalassiosira weissflogii, Dunaliella salina, and Rhodomonas salina) and nutrient-dependent shifts in stable isotope signatures in response to nutrient limitation of R. salina. The trophic enrichment in 15N and 13C of primary and secondary consumers (nauplii of Acartia tonsa and larval herring) showed strong deviations from the postulated degree of 1.0‰ enrichment in δ13C and 3.4‰ enrichment in δ15N. Surprisingly, nauplii of A. tonsa tended to keep “isotopic homeostasis” in terms of δ15N, a pattern not described in the literature so far. Our results suggest that the diets’ nutritional composition and food quality as well as the stoichiometric needs of consumers significantly affect the degree of trophic enrichment and that these mechanisms must be considered in ecological studies, especially when lower trophic levels, where variability is highest, are concerned.

Description: Semi Permeable Membrane Device (SPMD) was deployed on an experimental basis for five days inside an Environmental Chemistry Laboratory and two sites outside the building in Kiel, Germany to understand the time-averaged contaminant profiles and concentrations of PCBs. Multi Dimensional High Resolution Gas Chromatography-Electron Capture Detection technique and high resolution clean up techniques were employed to SPMD derived triolein samples. Air concentrations were derived from well established mass transfer coefficients or rate constants for PCBs in SPMD. PCBs profiles in indoor and outdoor samples were distinctly different, exemplified by a particle free clean-up laboratory facility where particle associated higher chlorinated congeners were absent. SPMDs revealed the ‘occupational hazard’ to workers inside the building from chemical contamination derived from both building materials and chemicals used in the laboratory. Finger printing technique using principle component analysis (PCA) revealed that PCB contamination was derived from German commercial PCB mixtures. SPMD derived air concentrations in outdoor samples resembled levels recorded by similar devices in Europe. Ultimately, a simple sampling technique in combination with high resolution analytical techniques demonstrated the uptake of more than 60 PCB congeners within a short period of time.

Description: Cyclone activity and life cycle are analysed in the coupled GCMs ECHAM5/OM and ECHAM4/OPYC3. First, the results for the present climate (1978–1999) are compared with ERA-40 and NCEP/NCAR reanalyses, showing a drastic improvement in the representation of cyclone activity in ECHAM5/OM compared to ECHAM4/OPYC3. The total number of cyclones, cyclone intensity, propagation velocity and deepening rates are found to be much more realistic in ECHAM5/OM relative to ECHAM4/OPYC3. Then, changes in extra tropical cyclone characteristics are compared between present day climate and future climate under the emission-scenario A1B using ECHAM5/OM. This comparison is performed using the 20-year time slices 1978–1999, 2070–2090 and 2170–2190, which were considered to be representative for the various climate conditions. The total number of cyclones does not undergo significant changes in a warmer climate. However, regional changes in cyclone numbers and frequencies are evident. One example is the Mediterranean region where the number of cyclones in summer increases almost by factor 2. Some noticeable changes are also found in cyclone life cycle characteristics (deepening rate and propagation velocity). Cyclones in the future climate scenario tend to move slower and their deepening rate becomes stronger, while cyclone intensity does not undergo significant change in a warmer climate. Generally, our results do not support the hypothesis of enhanced storminess under future climate conditions

Description: This study should clarify the importance of morphology and stability of the mandibular gnathobases for the diet of Antarctic copepod species. The gnathobase morphology of the dominant copepod species Calanoides acutus, Calanus propinquus, Ctenocalanus citer, Rhincalanus gigas, Metridia gerlachei, Stephos longipes, Microcalanus pygmaeus and Paraeuchaeta antarctica from the Southern Ocean was investigated in detail by means of a scanning electron microscope. The mandibular gnathobases of C. acutus, C. propinquus and C. citer have relatively short and compact teeth. These species feed mainly on diatoms and are able to crack the silicious diatom frustules with their mandibular gnathobases by directed pressure. In contrast the teeth of the mandibular gnathobases of P. antarctica are very long and pointed. The nutrition of this species consists predominantly of other smaller copepod species. The motile prey can be held by skewering, using the gnathobases, and then eventually minced. The mandibular gnathobases of P. antarctica have notably more small bristles than those of the other investigated copepod species. These bristles are probably associated with receptors and could serve to locate the prey. The morphology of the gnathobases of R. gigas and M. gerlachei is between that of P. antarctica on the one side and that of C. acutus, C. propinquus and C. citer on the other. Based on the morphology of its gnathobases the copepod species S. longipes, which has to date been found to feed primarily on phytoplankton, mainly ice algae, must also be considered a zooplankton feeder. The investigation showed that M. pygmaeus has gnathobases with surprisingly long and pointed teeth, indicating that this species very probably feeds both on phyto- and on zooplankton organisms. While the mandibular gnathobases of the males of C. propinquus, R. gigas, M. gerlachei and S. longipes have the same morphology as the females of the respective species, in the other four investigated copepod species the males have reduced (C. acutus, C. citer and M. pygmaeus) or completely missing mandibular gnathobases (P. antarctica). The teeth of the gnathobases of all studied species with the exception of M. gerlachei consist of a different material than the remaining parts of the gnathobases. This material seems to be silicate, which probably enhances the stability of the gnathobase teeth.

Description: Lipid content, fatty acid composition, and feeding activity of the dominant Antarctic copepods, Calanoides acutus, Calanus propinquus, and Metridia gerlachei, were studied at a quasi-permanent station in the eastern Weddell Sea in December 2003. During 3 weeks of the spring phytoplankton development, total lipid levels of females and copepodite stages V (CVs) of C. acutus were almost doubled. Meanwhile, only a slight increase in total lipid content occurred in M. gerlachei, and no clear trend was observed in lipids of C. propinquus females. The pronounced increase of lipids in C. acutus was due to an accumulation of wax esters. The proportion of wax esters in the lipids of M. gerlachei was clearly lower, while triacylglycerols played a more important role. In C. propinquus, triacylglycerols were the only neutral lipid class. There were no pronounced changes in the feeding activity of M. gerlachei, whereas the feeding activity of C. acutus had rapidly increased with the development of the phytoplankton bloom in December, which explains its rapid lipid accumulation. The combination of gut content and fatty acid trophic marker analyses showed that C. acutus was feeding predominantly on diatoms. The typical diatom fatty acid marker, 16:1(n-7), slightly decreased and the tracer for flagellates, 18:4(n-3), increased in females and CVs of C. acutus. This shift indicates the time, when the significance of flagellates started to increase. The three copepod species exhibited different patterns of lipid accumulation in relation to their trophic niches and different duration of their active phases. The investigations filled a crucial data gap in the seasonal lipid dynamics of dominant calanoid copepods in the Weddell Sea in December and support earlier hypotheses on their energetic adaptations and life cycle strategies.

Description: A regional classification of shoreline segments along the Alaskan Beaufort Sea Coast was developed as the basis for quantifying coastal morphology, lithology, and carbon and mineral sediment fluxes. We delineated 48 mainland segments totaling 1,957 km, as well as 1,334 km of spits and islands. Mainland coasts were grouped into five broad classes: exposed bluffs (313 km), bays and inlets (235 km), lagoons with barrier islands (546 km), tapped basins (171 km) and deltas (691 km). Sediments are mostly silts and sands, with occasional gravel, and bank heights generally are low (2–4 m), especially for deltas (〈1 m). Mean annual erosion rates (MAER) by coastline type vary from 0.7 m/year (maximum 10.4 m/year) for lagoons to 2.4 m/year for exposed bluffs (maximum 16.7 m/year). MAERs are much higher in silty soils (3.2 m/year) than in sandy (1.2 m/year) to gravelly (−0.3 m/year) soils. Soil organic carbon along eroding shorelines (deltas excluded) range from 12 to 153 kg/m2 of bank surface down to the water line. We assume carbon flux out from depositional delta sediments is negligible. Across the entire Alaskan Beaufort Sea Coast, estimated annual carbon input from eroding shorelines ranges from –47 to 818 Mg/km/year (Metric tones/km/year) across the 48 segments, average 149 Mg/km/year (for 34 nondeltaic segments), and total 1.8×105 Mg/year. Annual mineral input from eroding shorelines ranges from −1,863 (accreting) to 15,752 Mg/km/year, average 2,743 Mg/km/year, and totals 3.3 ×106 Mg/year.

Description: 18.10. - 25.10.2011

Description: The results of permafrost and coastal dynamics investigations at four key sites on the shores of the Kara and Barents Seas are discussed. Three ACD key sites, Marre-Sale, Shpindler, and Kolguev, characterize areas with active thermal erosion; key site Cape Bolvansky is found on a relatively stable coast. It is found that the coastal retreat rate has spatial and temporal variability, which is typical of the entire Arctic coast. Coastal deposits on the Kara and Barents Seas have a low organic carbon content. Annual input of material into the Kara Sea resulting from coastal degradation reaches 35–40 million t, including about 7.5 million t of ice, 0.35 million t of organic carbon, and 0.3 million t of soluble salts.

Description: We propose a new method for obtaining average velocities and eddy diffusivities from Lagrangian data. Rather than grouping the drifter-derived velocities in geographical bins, we group them by nearest-neighbor distance using a clustering algorithm. This yields sets with approximately the same number of observations, covering unequal areas. A major advantage is that, because the number of observations is the same for the clusters, the statistical accuracy is more uniform than with geographical bins. We illustrate the technique using synthetic data from a stochastic model, employing a realistic mean flow. The latter represents the surface currents in the Nordic Seas and is strongly inhomogeneous in space. We use the clustering algorithm to extract the mean velocities and diffusivities and compare the results with the corresponding quantities from the stochastic model. We perform a similar comparison with the means and diffusivities obtained with geographical bins. Clustering is more successful at capturing the mean flow and improves convergence in the eddy diffusivity estimates. We discuss both the advantages and shortcomings of the new method.

Description: According to available historical evidence, colonization of the White Sea region began in the 9th–8th centuries B.C. [2]. This unique region with ancient residence sites experiences progressively increasing anthropogenic load against the background of natural climatic changes. The intense economic development of the region requires complex ecological and paleogeographic studies aimed at detailed reconstruction of past sedimentation settings. The analysis of dinocysts, which makes it possible to reconstruct various parameters of water masses washing the Arctic shelf, has become a promising method in paleohydrological studies, widely used in recent years [10, 11]. Dinoflagellates, which represent one of the main phytoplankton groups in the Arctic Seas, the White Sea included, form cysts with biopolymer envelopes, which are preserved in sediments. Thus, information on glacial–hydrological conditions is recorded in marine sediments. In the White Sea, this method was first used for the study of bottom sediments only in 2003 [3, 15]. In this communication, we present the first results obtained during the thorough study of dinocysts in bottom sediment cores from the White Sea, which cover the last 250 years. The study of this microfossil group made it possible to reconstruct in detail changes in glacial–hydrological settings in the sea and reveal their relations with known climatic–hydrological events that occurred in neighboring regions during the historical period.

Description: The influence of the natural variability of the Atlantic meridional overturning circulation (AMOC) on the atmosphere is studied in multi-centennial simulations of six global climate models, using Maximum Covariance Analysis (MCA). In all models, a significant but weak influence of the AMOC changes is found during the Northern Hemisphere cold-season, when the ocean leads the atmosphere by a few years. Although the oceanic pattern slightly varies, an intensification of the AMOC is followed in all models by a weak sea level pressure response that resembles a negative phase of the North Atlantic Oscillation (NAO). The signal amplitude is typically 0.5 hPa and explains about 10% of the yearly variability of the NAO in all models. The atmospheric response seems to be due primarily due to an increase of the heat loss along the North Atlantic Current and the subpolar gyre, associated with an AMOC-driven warming. Sea-ice changes appear to be less important. The stronger heating is associated to a southward shift of the lower-tropospheric baroclinicity and a decrease of the eddy activity in the North Atlantic storm track, which is consistent with the equivalent barotropic perturbation resembling the negative phase of the NAO. This study thus provides some evidence of an atmospheric signature of the AMOC in the cold-season, which may have some implications for the decadal predictability of climate in the North Atlantic region.

Description: This work is the first detailed description of the Late Pleistocene-Holocene and Recent Ostracoda of the Laptev Sea. A total of 45 species in 22 genera and 13 families have been identified. All these species are described monographically. Three different ecological assemblages of ostracodes corresponding to different combinations of environmental parameters have been established; they are restricted to three regions of the sea: western-central, eastern, and southern. The recent ostracode assemblages of the Laptev Sea have been compared with those from other Arctic areas and are most similar to those of the Beaufort and Kara seas. Data on recent Ostracoda are used for paleoenvironmental reconstructions on the eastern shelf and western continental slope of the Laptev Sea. For this purpose, ostracodes from five sections obtained from these parts of the sea have been examined. The oldest sediments, which are of Late Pleistocene age (15.8 cal. ka BP), have been recovered in a core from the western continental slope. These yielded five ostracode assemblages, which correspond to different paleoenvironments and replaced each other in the course of the rapid postglacial sea-level rise, thus showing variations in the Atlantic water inflow from the west and freshwater discharge from the subaerially exposed shelf. On the outer shelf of the eastern part of the sea, the rapid sea-level rise in the Early Holocene (lowermost dating 11.3 cal. ka BP) led to a rapid transition from assemblages of brackish-water near-shore environments to those of modernlike normal marine environments; modern environments were established about 8.2 cal. ka ago. Since the core sections from the inner shelf correspond to the time when the level of the sea had already reached its modern values, the changes in the taxonomic composition of ostracode assemblages primarily mirror variations in river runoff.

Description: Ostracode analysis was carried out on samples from ice-rich permafrost deposits obtained on the Bykovsky Peninsula (Laptev Sea). A composite profile was investigated that covers most of a 38-m thick permafrost sequence and corresponds to the last ca. 60 kyr of the Late Quaternary. The ostracode assemblages are similar to those known from European Quaternary lake deposits during cold stages. The ostracode habitats were small, shallow, cold, oligotrophic pools located in low centred ice wedge polygons or in small thermokarst depressions. In total, 15 taxa, representing 7 genera, were identified from 65 samples. The studied section is subdivided into six ostracode zones that correspond to Late Quaternary climatic and environmental stadial-interstadial variations established by other paleoenvironmental proxies: (1) cold and dry Zyrianian stadial (58–53 kyr BP); (2) warm and dry Karginian interstadial (48–34 kyr BP); (3) transition from the Karginian interstadial to the cold and dry Sartanian stadial (34–21 kyr BP); (4) transition from the Sartanian stadial to the warm and dry Late Pleistocene period, the Allerød (21–14 kyr BP); (5) transition from the Allerød to the warm and wet Middle Holocene (14–7 kyr BP); and (6) cool and wet Late Holocene (ca. 3 kyr BP). The abundance and diversity of the ostracodes will be used as an additional bioindicator for paleoenvironmental reconstructions of the Siberian Arctic.

Description: The Kola Peninsula is one of the key areas for the study of postglacial development of northern Europe. The repeated formation of ice shields during the Pleistocene determined the peculiar features of the relief, structure of Quaternary sediments, and recent landscapes of the peninsula. The paleogeographic reconstructions available for the last 10–11 ka are based on results of the palynological analysis of lacustrine and boggy sediments [3–5, 9–11] and, to a lesser extent, on diatoms from the lacustrine sediments [7, 8]. In this communication, we present the first data on diatom assemblages from peat sections of the central Kola Peninsula and their detailed radiocarbon ages. The high sensitivity of lacustrine–boggy ecosystems to environmental changes made it possible to define several Holocene diatom assemblages and to correlate their succession with regional climatic events and variations in the local base level.

Description: Subfossil Cladocera were sampled and examined from the surface sediments of 35 thermokarst lakes along a temperature gradient crossing the tree line in the Anabar-river basin in northwestern Yakutia, northeastern Siberia. The lakes were distributed through three environmental zones: typical tundra, southern tundra and forest tundra. All lakes were situated within the continuous permafrost zone. Our investigation showed that the cladoceran communities in the lakes of the Anabar region are diverse and abundant, as reflected by taxonomic richness, and high diversity and evenness indices (H = 1.89 ± 0.51; I = 0.8 ± 0.18). CONISS cluster analysis indicated that the cladoceran communities in the three ecological zones (typical tundra, southern tundra and forest-tundra) differed in their taxonomic composition and structure. Differences in the cladoceran assemblages were related to limnological features and geographical position, vegetation type, climate and water chemistry. The constrained redundancy analysis indicated that TJuly, water depth and both sulphate (SO4 2−) and silica (Si4+) concentrations significantly (p ≤ 0.05) explained variance in the cladoceran assemblage. TJuly featured the highest percentage (17.4 %) of explained variance in the distribution of subfossil Cladocera. One of the most significant changes in the structure of the cladoceran communities in the investigated transect was the replacement of closely related species along the latitudinal and vegetation gradient. The results demonstrate the potential for a regional cladoceran-based temperature model for the Arctic regions of Russia, and for and Yakutia in particular.

Description: A large monothalamous foraminiferan, Toxisarcon taimyr sp. nov., has been isolated from the benthic samples from the Kara Sea inner shelf near the mouth of Yenisey river estuary, at a depth of 50–100 m. In its overall morphology, the new species closely resembles T. synsuicidica, one of the two species of Toxisarcon described to date. It possesses a large irregularly shaped cell body, covered by a thin layer of a fibrous organic coating. Numerous reticulopodia typically extend from all over the cell surface; the species is very motile and rapidly changes cell shape. Long and thick reticulopodial bundles form in the direction of movement. In the phylogenetic tree based on partial small-subunit ribosomal DNA (SSU rDNA) sequences, T. taimyr branches together with the two other known species of Toxisarcon within the clade C of monothalamous foraminifera.

Description: The Hydrograph model (a distributed process-based model) was applied to the simulation of soil freeze-thaw and runoff processes, to assess the viability of the model approach and the influence of specific environmental factors in a permafrost environment. Three mountainous permafrost watersheds were studied, at the Kolyma Water Balance Station in north-eastern Russia. The watersheds include rocky talus, mountainous tundra and moist larch-forest landscape regimes, and they were modelled at daily time-steps for the period 1971–1984. Simulated results of soil freeze-thaw depth and runoff showed reasonable agreement with observed values. This study reveals and mathematically describes the dependence of surface and subsurface flow on thawing depth and landscape characteristics. Process analysis and modelling in permafrost regions, including ungauged basins, is suggested, with observable properties of landscapes being used as model parameters, combined with an appropriate level of physically based conceptualization.

Description: A regional forest carbon budget accounting technique based on carbon pools balance with incomes from growing woodstocks and losses from harvesting, fires and other disturbances have been developed. Forest carbon budgets of the Russian administrative units during 1988–2009 have been accounted. The carbon sink to Russian forests have increased from 80 Mt C × yr−1 in 1988 to 230–240 Mt C × yr−1 in late 2000s. This tendency is explained with the decline in harvesting, which have started in 1990s. European part of Russia was found to have higher areally averaged carbon sink compared with the Asian part. It have been associated with peculiar ways of wildfires governance in these two parts.

Description: Homoscleromorph sponges such as Oscarella spp. are characterized by unique morphological features, and Homoscleromorpha were therefore recently proposed as the fourth class of sponges. The microbiology of these sponges was mainly studied by electron microscopy while molecular studies are scarce. The aim of this study was to characterize the bacteria in Oscarella sponges using molecular tools. Denaturing gradient gel electrophoresis revealed distinct bacterial profiles in five Oscarella species and several color morphs of Oscarella lobularis. These profiles are characteristic of low microbial abundance (LMA) sponges. This was further confirmed by analysis of a 16S rRNA clone library from O. lobularis that yielded a low phylum-level diversity with dominance of Alphaproteobacteria. Bacterial communities in O. lobularis were very similar among different individuals (collected at the same site and time), five different color morphs, and specimens from different depths and locations, indicating a species-specific association. These results allow novel insights into the microbiology of the first known LMA sponge genus within the new class Homoscleromorpha.

Description: Comparison of five deep-water coral (DWC)/mound ecosystems along the European Continental Margin shows that suspended particulate organic matter (sPOM), a potential food source, is lipid rich and of high quality. However, there are differences between the sites. The Darwin and Pelagia Mounds (N. Rockall Trough and N. Porcupine Bank, respectively) have higher proportions of labile particulate lipids (including high proportions of polyunsaturated fatty acids) in the benthic boundary layer than Logachev, Hovland and Belgica Mounds (Rockall Bank, S. Porcupine Bank and Porcupine Seabight, respectively). The high quality sPOM could be transported downslope from the euphotic zone. There is some evidence for inter-annual variability at some sites (e.g. Hovland and Logachev Mounds) as large differences in suspended lipid and particulate organic carbon concentrations were observed over the sampling period. Elevated total organic carbon contents of sediments at mound sites, relative to control sites in some cases (particularly Darwin Mounds), probably reflect local hydrodynamic control and the trapping of sPOM by the DWC. Fresh POM can be relatively rapidly transferred to significant depth (up to 8 cm) through bioturbation that is evident at all sites. There is no clear evidence of present day hydrocarbon seepage at any of the sites.

Description: Submarine slope failures of various types and sizes are common along the tectonic and seismically active Ligurian margin, northwestern Mediterranean Sea, primarily because of seismicity up to ~M6, rapid sediment deposition in the Var fluvial system, and steepness of the continental slope (average 11°). We present geophysical, sedimentological and geotechnical results of two distinct slides in water depth 〉1,500 m: one located on the flank of the Upper Var Valley called Western Slide (WS), another located at the base of continental slope called Eastern Slide (ES). WS is a superficial slide characterized by a slope angle of ~4.6° and shallow scar (~30 m) whereas ES is a deep-seated slide with a lower slope angle (~3°) and deep scar (~100 m). Both areas mainly comprise clayey silt with intermediate plasticity, low water content (30–75 %) and underconsolidation to strong overconsolidation. Upslope undeformed sediments have low undrained shear strength (0–20 kPa) increasing gradually with depth, whereas an abrupt increase in strength up to 200 kPa occurs at a depth of ~3.6 m in the headwall of WS and ~1.0 m in the headwall of ES. These boundaries are interpreted as earlier failure planes that have been covered by hemipelagite or talus from upslope after landslide emplacement. Infinite slope stability analyses indicate both sites are stable under static conditions; however, slope failure may occur in undrained earthquake condition. Peak earthquake acceleration from 0.09 g on WS and 0.12 g on ES, i.e. M5–5.3 earthquakes on the spot, would be required to induce slope instability. Different failure styles include rapid sedimentation on steep canyon flanks with undercutting causing superficial slides in the west and an earthquake on the adjacent Marcel fault to trigger a deep-seated slide in the east.

Description: This study focuses on bioerosion of an aphotic deep-water coral mound, the Propeller Mound, in the northern Porcupine Seabight. The predominant framework builder is the cosmopolitan cold-water coral Lophelia pertusa. We demonstrate bioerosion patterns within the skeleton of L. pertusa using a new embedding method under vacuum conditions with subsequent scanning electron microscope analysis. Following this method, 23 ichnospecies are documented and related to heterotrophic organism groups such as Bacteria (1), Fungi (12), Bryozoa (1), Foraminifera (3), and Porifera (6). Predominant endolithic sponges in the framework of L. pertusa are Alectona millari and Spiroxya heteroclita. Owing to its characteristic growth and surface ornamentation, trace casts of Spiroxya heteroclita are correlated to the well-known trace fossil Entobia laquea. Investigations of thin sections of post-mortem skeletons show a clearly pronounced endolithic tiering of three penetration depths. The analysed samples are divided into three macroscopic preservational stages differing in post-mortem age, and exposure of the framework. Bioerosion affects bare parts of the coral skeleton. Bioeroders preferably settle on one side of an upright growing colony. A succession usually starts with the infestation by bacteria and fungi. Contact zones of epiliths are preferred areas for penetration by endoliths. Sponges and foraminifers appear 10 cm below the zone of living polyps, followed by boring bryozoans 15 cm below. However, in one case the sponge Spiroxya heteroclita is documented in the skeleton of living polyps. Frameworks exposed to water host 19 ichnospecies, thus forming the most diverse ichnocoenosis, whereas nine ichnospecies are documented in coral specimens buried by sediment. Mapping of epi- and endoliths in living and freshly necrotic colonies represents a useful tool for monitoring environmental conditions and define ecological “health” of deep-water corals in a rapid large-scale assessment of the state of coral reefs.

Description: Deep-water corals are widespread in the North Atlantic. Colonial azooxanthellate scleractinians sustain ecosystems mostly in the bathyal zone down the slopes and oceanic banks off the Iberian Peninsula to as far north as the Scandinavian shelf off northern Norway. Estimates of the geological age of 37 deepwater corals exposed at the seabed from major reef areas in the North Atlantic were based on U/Th datings. In contrast to the purely Holocene ages of deep-water corals in Scandinavian waters, the Faroe area and the Rockall Trough, deep-water corals from lower latitudes like the seamounts off NW-Africa, the Mid-Atlantic Ridge and the western Mediterranean Sea seemed to have grown continuously over the last 50 ka. Overall, deep-water corals showed U/Th ages between 0.09 and 53.5 ka.

Description: Colonial non-zooxanthellate corals from deep-water coral reefs, Lophelia pertusa and Madrepora oculata, produce large amounts of extracellular mucus (EMS). This mucus has various functions, e.g., an antifouling capability protecting the coral skeleton from attacks of endolithic and boring organisms. Both corals show thick epithecal and exothecal skeletal parts with a clear lamellar growth pattern. The formation of the epitheca is unclear. It is supposed that the EMS play a central role during the calcification process of the epithecal skeletal parts. Staining with the fluorochrome tetracycline has shown an enrichment of Ca2+ ions in the mucus. In order to investigate this hypothesis, the protein content of the mucus and the intracrystalline organic matter from newly formed epithecal aragonite of Madrepora oculata was determined via sodium dodecyl sulfate (SDS) gel electrophoresis. Identical band patterns within both substances could be detected, one around 45 kDa molecular weight and a cluster around 30-35 kDa molecular weight. The occurrence of identical protein patterns within the mucus and in the newly formed aragonite confirms the idea that the mucus plays an important role during the organomineralization of the coral epitheca.

Description: In spite of the fundamental role the Atlantic Meridional Overturning Circulation (AMOC) plays for global climate stability, no direct current measurement of the Denmark Strait Overflow, which is the densest part of the AMOC, has been available until recently that resolve the cross-stream structure at the sill for long periods. Since 1999, an array of bottom-mounted acoustic instruments measuring current velocity and bottom-to-surface acoustic travel times was deployed at the sill. Here, the optimization of the array configuration based on a numerical overflow model is discussed. The simulation proves that more than 80% of the dense water transport variability is captured by two to three acoustic current profilers (ADCPs). The results are compared with time series from ADCPs and Inverted Echo Sounders deployed from 1999 to 2003, confirming that the dense overflow plume can be reliably measured by bottom-mounted instruments and that the overflow is largely geostrophically balanced at the sill.

Description: Several cold vents are observed at the northern Cascadia margin offshore Vancouver Island in a 10 km2 region around Integrated Ocean Drilling Program Expedition 311 Site U1328. All vents are linked to fault systems that provide pathways for upward migrating fluids and at three vents methane plumes were detected acoustically in the water column. Downhole temperature measurements at Site U1328 revealed a geothermal gradient of 0.056 ± 0.004°C/m. With the measured in situ pore-water salinities the base of methane hydrate stability is predicted at 218–245 meters below seafloor. Heat-probe measurements conducted across Site U1328 and other nearby vents showed an average thermal gradient of 0.054 ± 0.004°C/m. Assuming that the bottom-simulating reflector (BSR) marks the base of the gas hydrate stability zone variations in BSR depths were used to investigate the linkages between the base of the gas hydrate stability zone and fluid migration. Variations in BSR depth can be attributed to lithology-related velocity changes or variations of in situ pore-fluid compositions. Prominent BSR depressions and reduced heat flow are seen below topographic highs, but only a portion of the heat flow reduction can be due to topography-linked cooling. More than half of the reduction may be due to thrust faulting or to pore-water freshening. Distinct changes in BSR depth below seafloor are observed at all cold vents studied and some portion of the observed decrease in the BSR depth was attributed to fault-related upwelling of warmer fluids. The observed decrease in BSR depth below seafloor underneath the vents ranges between 7 and 24 m (equivalent to temperature shifts of 0.07–0.15°C).

Description: The Northern Pacific seastar, Asterias amurensis, is a benthic marine predator, which has recently established several invasive populations in Australian waters. To investigate population structure, diversity and patterns of connectivity, we isolated and characterised 27 microsatellite loci and tested their polymorphism based on 46 individuals from two invasive populations. The mean allelic richness was 4.33; observed heterozygosity was 0.42, while the percentage of polymorphic loci was 92.6%. The polymorphic markers will prove useful in the assessment of population genetic parameters, in both invasive and native A. amurensis populations.

Description: M. Dunbar (1957) called attention to the existence of a small circular polynya about 50 m in diameter in Cambridge Fiord in northern Baffin Island for which there was no obvious explanation. Other small polynyas are known in arctic fjords which are usually the result of turbulent mixing in areas of strong currents (Sadler 1974), but Cambridge Fiord is 100 km long, its tidal range is small, and the polynya is situated within 300 m of the delta face at the head of the fjord so that strong turbulence is very unlikely. The annual reappearance of the polynya in late winter is confirmed by a series of aerial survey photographs taken by the Royal Canadian Air Force between 1952 and 1957 and also by reports from Inuit hunters from Pond Inlet. It is first seen within about two weeks of 15 March appearing in exactly the same position each year as a circle with a diameter of about 40 m. Over a period of about a week, a lead extends from the polynya to the shore and open water is visible in the tide crack for several hundred metres either side of the shore end of the lead. The lead, unlike the polynya, changes its position from year to year, but once formed it remains fixed until general break-up (Figure 1).

Description: This Encyclopedia comprises the current knowledge in marine geosciences whereby not only basic but also applied and technical sciences are covered. Through this concept a broad scale of users in the field of marine sciences and techniques is addressed, from students and scholars in academia to engineers and decision makers in industry and politics. Globally growing demand of energy and mineral resources, reliable future projection of climate processes and the protection of coasts to mitigate the threats of disasters and hazards require a comprehensive understanding of the structure, ongoing processes and genesis of the marine geosphere. Beyond the “classical” research fields in marine geology in current time more general concepts have been evolved integrating marine geophysics, hydrography, marine biology, climatology and ecology. As an umbrella the term “marine geosciences” has been broadly accepted for this new complex field of research and the solutions of practical tasks in the marine realm.

Description: Rhodopseudomonas globiformis is able to grow on sulfate as sole source of sulfur, but only at concentrations below 1 mM. Good growth was observed with thiosulfate, cysteine or methionine as sulfur sources. Tetrathionate supported slow growth. Sulfide and sulfite were growth inhibitory. Growth inhibition by higher sulfate concentrations was overcome by the addition of O-acetylserine, which is known as derepressor of sulfate-assimilating enzymes, and by reduced glutathione. All enzymes of the sulfate assimilation pathway. ATP-sulfurylase, adenylylphosphate-sulfotransferase, thiosulfonate reductase and O-acetylserine sulfhydrylase are present in R. globiformis. Sulfate was taken up by the cells and the sulfur incorporated into the amino acids cysteine, methionine and homocysteine. It is concluded, that the failure of R. globiformis to grow on higher concentrations of sulfate is caused by disregulation of the sulfate assimilation pathway. Some preliminary evidence for this view is given in comparing the activities of some of the involved enzymes after growth on different sulfur sources and by examining the effect of O-acetylserine on these activities.

Description: The ability to use adenosine 5′-phosphosulfate (APS) or 3′-phosphoadenosine 5′-phosphosulfate (PAPS) as the substrate for the initial reductive step in sulfate assimilation has been tested in most of the known Rhodospirillaceae species and in some chemotrophic bacteria. Improved and optimized methods for the synthesis and purification of the sulfonucleotides APS and PAPS are described. The production of acid volatile radioactivity from 35S-APS and 35S-PAPS was measured under various conditions in the presence and absence of non-labeled sulfate. Specific differences in the ability to reduce APS or PAPS were observed among the Rhodospirillaceae species and also the chemotrophic bacteria. APS was found to be the substrate of the thiolsulfotransferase in Rps. acidophila, Rps. globiformis, Rm. vannielii, Rc. purpureus, R. tenue, Rps. gelatinosa, in Alcaligenes eutrophus and Pseudomonas aeruginosa. PAPS was the substrate in Rps. capsulata, Rps. sphaeroides, Rps. sulfidophila, Rps. palustris, Rps. viridis, R. rubrum, R. fulvum, in Paracoccus denitrificans and in several Enterobacteriaceae. The presence of different enzymatic systems for sulfate reduction in the Rhodospirillaceae family is compared with their taxonomical grouping and their possible phylogenetic relatedness.

Description: The mechanism of sulfate assimilation was investigated in Rhodopseudomonas sulfidophila, a bacterium able to grow either photoautotrophically, with sulfide as electron donor, or photoheterotrophically with sulfate as sole sulfur source. ATP sulfurylase, adenosine-5′-phosphosulfate kinase, 3′-phosphoadenosine-5′-phosphosulfate sulfotransferase, thiosulfonate reductase and cysteine synthase were present. Reduced sulfur compounds, especially sulfide and sulfite repressed all steps of sulfate activation and reduction including sulfate uptake. Adenosine-5′-phosphosulfate kinase activity in contrast to the other activities was high in the presence of cysteine or reduced glutathione in the growth medium. Sulfur was incorporated into the cellular sulfolipid from sulfate and also from reduced sulfur compounds like cysteine and thiosulfate. The activity of 3′-phosphoadenosine-5′-phosphosulfate sulfotransferase was rapidly lost during gel filtration or dialysis. From comparison with other sulfotransferases and from the specific cofactor requirement for the enzyme of R. sulfidophila it is concluded that two different low molecular weight cofactors are required in this system. A reaction sequence is proposed involving thioredoxin as the reductant of another dialysable low molecular weight cofactor, that binds to the protein.

Description: A new purple nonsulfur bacterium was isolated from enrichment cultures of a sulfide-containing marine lagoon. The bacterium is similar to Rhodopseudomonas capsulata and is described as a new species of the genus Rhodopseudomonas: Rhodopseudomonas adriatica. Cells are non-motile, 0.5–0.8 μm by 1.3–1.8 μm, and multiply by binary fission. Intracytoplasmic membranes are of the vesicular type. The photosynthetic pigments are bacteriochlorophyll a and carotenoids of the spheroidene group. Growth is possible anaerobically in the light and at low pO2 in the dark. Biotin and thiamine are required as growth factors. A wide variety of organic compounds, as well as sulfide and thiosulfate, are used as photosynthetic electron donors. Sulfide is oxidized to elemental sulfur, which is subsequently converted to sulfate, whereas thiosulfate oxidation occurs without measurable intermediate. Rhodopseudomonas adriatica is unable to assimilate sulfate, growth is only possible in the presence of a reduced sulfur compound.

Description: Seven strains of five species of the genus Ectothiorhodospira were characterized by oligonucleotide cataloguing of their 16S rRNA in order to determine the phylogenetic relationship to one another and to other phototrophic purple bacteria. All representatives of Ectothiorhodospira are members of that line of descent defined by phototrophic purple sulfur bacteria and relatives, showing a moderate relationship to those phototrophic organisms forming globules of elemental sulfur inside the cell (Chromatium and relatives). The 5 Ectothiorhodospira species fall into two subgroups. E.halophila, E. halochloris and E. abdelmalekii form one, E. mobilis, E. shaposhnikovii and the unnamed strain BN 9906 form the second subgroup. Within the two subgroups the strains are closely related, while the degree of relatedness found between members of the two subgroups is more distant.

Description: Oceanic eddies having scales from several hundred meters to several hundred kilometers are ubiquitous phenomena in the World’s ocean. This became evident only after they could be observed from satellites and space shuttles. Here we present several images taken in different spectral bands which show signatures of eddies of different spatial scales in sea areas around Africa. In particular, we present a series of satellite images showing the propagation of a small-scale cyclonic (cold) eddy generated at Cap-Vert at the coast of Senegal into the open ocean. We show that this small-scale eddy transported nutrients from the Senegal upwelling region westward into the oligotrophic North Atlantic thus giving rise to enhanced chlorophyll-a concentration there. Since eddies are also areas of high fish population, knowledge of their position and properties is of great importance for fishery.

Description: The geoecological situation in the regions of intense industrial exploitation on the Pechora Sea coast, particularly in the Varandei area, is dangerous. Human factors intensify eolian and slope processes and thermoerosion. Coastal stability decreases and coastal retreat rates are twice as high as in regions unaffected by human activity. Industrial exploitation results in the destruction of natural environments and considerable material losses. Several housing estates and industrial constructions have already been destroyed because of coastal erosion. Damage increases each year as the cliff retreats towards the center of the Varandei settlement. The oil terminal, airport and other industrial objects are also endangered.

Description: The experiment IGLOS (Investigation of the Greenland Boundary Layer Over Summit) was conducted in June and July 2002 in the central plateau of the Greenland inland ice. The German research aircraft Polar2, equipped with the turbulence measurement system Meteopod, was used to investigate turbulence and radiation flux profiles near research station “Summit Camp”. Aircraft measurements are combined with measurements of radiation fluxes and turbulent quantities made from a 50 m tower at Summit Camp operated by Eidgenössische Technische Hochschule (ETH) Zürich. During all six flight missions, well-developed stable boundary layers were found. Even in high-wind conditions, the surface inversion thickness did not exceed roughly 100 m. The turbulent height of the stable boundary layer (SBL) was found to be much smaller than the surface inversion thickness. Above the surface layer, significant turbulent fluxes occurred only intermittently in intervals on the order of a few kilometres. Turbulent event fraction in the upper SBL shows the same dependence on gradient Richardson number as reported for near-surface measurements. Clear-air longwave radiation divergence was always found to contribute significantly to the SBL heat budget. In low-wind cases, radiative cooling even turned out to be dominant.

Description: Radiative transfer modelling for a coupled ocean-atmosphere system near 685 nm indicates a sufficiently high fluorescence signal of the chlorophyll a transmitted to the top of the atmosphere. However, only the shortwave half is seen at the top of the atmosphere, the longwave half is fully masked by atmospheric oxygen and water vapour absorption. The impact of atmospheric aerosol extinction on the signal transmission is almost negligible. The Hα line of the sun, atmospheric water vapour absorption, and chlorophyll absorption near 670 nm influence radiative transfer in the shortwave tail of the fluorescence line making the search for a baseline in order to eliminate the background radiation a difficult task.

Description: The success of the 1st International Symposium on Sponge Microbiology reflects the growing interest of the scientific community in this new and emerging field. Research themes of the symposium included symbiont diversity, physiology and function, secondary metabolites, metagenomics, single-cell genomics and other -omics approaches, sponge–symbiont interactions, sponge diseases, environmental stress, and many more. This article summarizes the major developments in the field and identifies future foci for research.

Description: Water temperature has received considerable attention as steering factor for the genesis of different types of marine carbonate sediments. However, parameters other than temperature also strongly influence ecosystems and, consequently, the carbonate grain associations in the resulting carbonate rock. Among those factors are biological evolution, water energy, substrate, water chemistry, light penetration, trophic conditions, CO2 concentrations, and Mg/Ca ratios in the seawater. Increased nutrient levels in warm-water settings, for example, lead to heterotrophic-dominated associations that are characteristic of temperate to cool-water carbonates. Failure to recognize the influence of such environmental factors that shift the grain associations towards heterotrophic communities in low latitudes can lead to misinterpretation of climatic conditions in the past. Modern analogues of low-latitude heterozoan carbonates help to recognize and understand past occurrences of heterozoan warm-water carbonates. Careful analysis of such sediments therefore is required in order to achieve robust reconstructions of past climate.

Description: Previous studies have revealed silica formation in the teeth of mandibular gnathobases of copepods while significant amounts of zinc and copper are present, which might improve mechanical stability of the teeth and represent an adaptation to compact food particles. The present study aimed at analysing the distribution and concentration of trace elements in the mandibular gnathobases of females of the Antarctic copepod species Calanoides acutus. Because of the low overall masses of few micrograms per specimen the application of a combination of position-resolved micro-beam techniques was necessary and micro-particle-induced X-ray emission spectrometry and laser ablation inductively coupled plasma mass spectrometry were used to determine Ba, Br, Ca, Cl, Cu, Fe, K, Mg, Na, Ni, P, S, Si and Zn in the samples with μm to sub-μm resolution. Calibration strategies were optimised to fit for the carbonate matrix. The analyses revealed a distinct enrichment of Br, Ca, Fe, K, S, Si and Zn in the teeth of the gnathobases.

Description: The authors describe in The Russian North Pole Drifting Stations for the first time the history of establishing drifting stations in the Arctic Basin since 1937. They set out the main aims and goals of the observations made and outline the methods of organizing the drifting stations, gear and equipment for life support and scientific observations at the North Pole drifting stations and during the airborne high-latitudinal expeditions. The main scientific results and the analysis of data obtained during metereological, oceanographic, ice and geophysical observations are presented and the book contains illustrations, maps and tables that can be used by a wide range of specialists investigating the nature of the Arctic region. An analysis of the contribution of the data collected at the drifting stations, the process of envionmental research in the Arctic Basin and the plans for future use of drifting stations is provided, with special emphasis on the forthcoming International Polar Year 2007-2008.

Description: The main migrations of the Polar front (PF) during the last 300 ka were identified using planktic foraminiferal census data and derived from them sea surface paleotemperature (SST) estimates in two synchronized AMK-4438 and M23414 cores recovered directly beneath the main stream of the North Atlantic Current (NAC) south of Iceland. During the summer seasons, the cold waters adjacent to the PF did not reach the studied sites. These waters occurred here only during the winter seasons of MIS 2, 6, and 8. The northern part of the study area was influenced by the arctic waters more often than its southern part. During MIS 8 and 6 isotherms in the North Atlantic had mainly the subzonal orientation, while during MIS 2-4 they had the submeridional orientation. During the interglacials, the PF was located northward and westward from the study area. During MIS 7, the front was presumably situated closer to the study area in comparison with its modern position, and the isotherms were oriented mainly subzonal. For the MIS 5e period, we observed the most distant retreat of PF from the investigated area in the western and northwestern direction in relation to the anomalous deflection of the NAC to the north-west (intensification of the Irminger current) and the predominance of the submeridional orientation of the isotherms in the study area. During MIS 1, as well as MIS 7, the isotherms in the study area had mainly the subzonal orientation.

Description: A set of digital maps including geology, Quaternary sediments, landscapes, engineering-geological, vegetation, geocryological and the series of regional sources have been selected to characterize the Russian Arctic coast. Based on this data, new maps of engineering geocryological zoning and zoning of the coast with respect to the intensity of exogenous geological processes and risk of technogenic impacts have been generated at the scales of 1:4,000,000–1:8,000,000. These maps are a tool to assess the impact of industry on the Arctic coast of the country.

Description: Chironomid, pollen, and rhizopod records from a permafrost sequence at Bol’shoy Lyakhovsky Island (New Siberian Archipelago) document the development of a thermokarst palaeo-lake and environmental conditions in the region during the last Interglacial (MIS 5e). Open Poaceae and Artemisia associations dominated vegetation at the beginning of the interglacial period. Rare shrub thickets (Salix, Betula nana, Alnus fruticosa) grew in more protected and wetter places as well. Saalian ice wedges started to melt during this time, resulting in the formation of an initial thermokarst water body. The high percentage of semi-aquatic chironomids suggests that a peatland-pool initially existed at the site. A distinct decrease in semi-aquatic chironomid taxa and an increase in lacustrine ones point to a gradual pooling of water in the basin, which could in turn induce thermokarst and create a permanent pond during the subsequent period. The highest relative abundance of Chironomus and Procladius reflects unfrozen water remaining under the ice throughout the ice-covered period during the later stage of palaeo-lake development. The chironomid record points to three successive stages during the history of the lake: (1) a peatland pool; (2) a pond (i.e., shallower than the maximum ice-cover thickness); and (3) a shallow lake (i.e., deeper than the maximum ice-cover thickness). The trend of palaeo-lake development indicates that intensive thermokarst processes occurred in the region during the last Interglacial. Shrub tundra communities with Alnus fruticosa and Betula nana dominated the vegetation during the interglacial optimum. The climate was moister and warmer than present. The results of this study suggest that quantitative chironomid-based temperature reconstructions from Arctic thermokarst ponds/lakes may be problematic due to other key environmental factors, such as prolonged periods of winter anoxia and local hydrological/geomorphological processes, controlling the chironomid assemblages.

Description: Methanogenic archaea from Siberian permafrost complementary to the already well-studied methanogens from non-permafrost habitats were exposed to simulated Martian conditions. After 22 days of exposure to thermo-physical conditions at Martian low- and mid-latitudes up to 90% of methanogenic archaea from Siberian permafrost survived in pure cultures as well as in environmental samples. In contrast, only 0.3%–5.8% of reference organisms from non-permafrost habitats survived at these conditions. This suggests that methanogens from terrestrial permafrost seem to be remarkably resistant to Martian conditions. Our data also suggest that in scenario of subsurface lithoautotrophic life on Mars, methanogenic archaea from Siberian permafrost could be used as appropriate candidates for the microbial life on Mars.

Description: Arctic coastal evolution is the result of interactions between exogenic and endogenic processes. In the arctic region, this evolution differs from that in other areas of the world’s oceans as a result of interactions between modern wave and ice factors, and the influences of glaciations and large-scale sea level changes in the past. Geologic structure, origin and development determine contemporary relief morphology. Morphology appears to be the most significant relief characteristic, but it is controlled by a set of interactive processes active over long periods. Our approach, in which a multitude of interacting factors are simultaneously analyzed and determined, could be called “morphogenetic”. We consider marine coasts and offshore zones (shelf) as a unit, and providing a general explanation for their evolution. The classification presented here is based upon the general approach given in the Science and Implementation Plan of Arctic Coastal Dynamics (ACD), a project of the International Arctic Science Committee and the International Permafrost Association. Our classification extends beyond the morphological ACD classification to include a morphogenetic classification.

Description: Three bottom sediment cores (140–190 cm long) taken from Isfjorden, West Spitsbergen, were analysed for pollen and spores with the main aim of elucidating the local pattern of pollen and spore succession in order to establish age control and define the stratigraphy of marine sediments. Isfjorden bottom sediments consist of greyish-green silty pelite with gruss, detritus, and pebble inclusions. The upper 25 cm are water-saturated and nonplastic. In spite of extremely low concentrations, and the predominance of reworked pre-Quaternary microfossils, the marine pollen spectra appear quite similar to those known from radiocarbon-dated lake sediments and peat exposures on the coasts of neighbouring Billefjorden, Van Mijenfjorden, and Hornsundfjorden, provided that long-distance transported pollen of conifers, tree birches, and spores of ferns are eliminated from marine pollen spectra compositions. The correlation of pollen zones (PZ) established in fjord sediments with those known from peat and lake sections enables the pollen-based stratigraphy of Isfjorden bottom sediments to be established and further reconstruction to be made of the major stages of the late Holocene terrestrial vegetation history of West Spitsbergen. The oldest pollen records date back to about 2.8–3 ka. They characterize the lowermost silty pelite layer (intervals 180–150 cm in core 11 and 190–60 cm in core 14 from the southwestern part of the fjord). At this time, moss–cereal–sedge fens and heather bogs in the coastal areas coexisted with rocky tundra vegetation at higher elevations. A marked increase in the content of Salix sp., Betula sect. Nanae-type, and Ericales pollen is recorded in the upper part of the pelite layer in cores 11 and 14. Similar spectra dominate core 9 from the northeastern inner part of the fjord. The percentage of green moss spores is extremely low. This type of spectra is suggestive of a warmer-than-present climate in West Spitsbergen. The upper water-saturated layer of all three cores contains pollen assemblages that are very similar to those identified in the Isfjorden surface sediment samples. Therefore, they have been likely accumulated during the last 2000 years. These uppermost pollen assemblages show a sharp increase in sedge pollen. This suggests the expansion of coastal fens, which can be attributed to an increase in the amount of precipitation. Extremely low pollen concentrations in Isfjorden bottom sediments possibly reflect very high accumulation rates during the time of silty pelite layer sedimentation.

Description: Lake Mogilnoe (Kildin Island, the Barents Sea) is a marine stratified lake, a refuge for landlocked populations of marine organisms. Unlike other known marine lakes from polar areas, which communicate with the sea by water percolation at the surface, Mogilnoe has a subterranean connection with the sea like tropical and subtropical anchialine lakes. Similarly to some other marine lakes, Mogilnoe has traditionally been considered to be biologically isolated from the sea and subject to little change. We review the current status of the physical features, zooplankton and benthos of Mogilnoe and trace changes that have occurred in the lake since the start of observations in 1894. The anaerobic bottom water layer has expanded by 100 %, while the upper freshwater layer has diminished by 40 %. The species diversity of zooplankton and macrobenthos has halved. The occurrence of Atlantic cod likens Mogilnoe to some other Arctic marine lakes while the presence of large flocks of sea anemones, scyphomedusae and suberitid sponges makes it similar to tropical anchialine lakes. Lake Mogilnoe is not entirely biologically isolated; accidental introduction of species from the sea does occur. We argue that the idealised model of an isolated steady-state ecosystem can be applied to a marine lake with caution. A model of fluctuating abiotic environment and partial biological isolation portrays the real situation better.

Description: Two sediment cores retrieved from the southern Lomonosov Ridge (LR) in 2007 (core ALR07-26C from the top of the ridge, water depth 1359 m, and core ALR07-15C from the base of Geophysicists’ Spur, water depth 2500 m) were investigated for lithology (wt % 〉 63 μm, terrigenous lithic grains 〉500 μm) and microfossils. Prominent peaks of coarse-grained material in ALR07-26C represented largely by quartz and clastic rocks are regarded as inputs of ice and, especially, iceberg-rafted debris (IRD) of Eurasian origin. In accordance with previously obtained evidence from age-constrained cores from the central LR, the highest peak 4 is correlated with the MIS 6–5 boundary and the disintegration of the Saalian ice sheet. The three younger IRD peaks are provisionally correlated with the MIS 5–4, MIS 4–3, and MIS 2–1 boundaries, respectively. Small peaks of coarse-grained material in ALR07-15C dominated by various rocks in contrast represent local material transported by downslope slides mixed with some IRD. No calcareous microfossils occur in the cores, but only agglutinated benthic foraminifers are found. In ALR07-26C, they correlate with IRD-rich layers, which correspond to glacial terminations with more open-sea ice conditions and, probably, higher productivity in the sea-ice marginal zone. The Cyclammina-dominated assemblage in ALR07-26C below IRD peak 4 supports the proposed age estimate for this peak (MIS 6–5), as similar foraminiferal assemblages in other LR cores are recorded in sediments of MIS 7–9 and older. Younger assemblages show a transition from a Recurvoides-dominated assemblage in the early Late Pleistocene to a more “oligotrophic” recent assemblage with a predominance of Reophax and Rhabdammina.

Description: Palytoxin (PTX), one of the most potent and chemically complex marine toxins, is predominantly found in zoanthid corals and sporadically in dinoflagellates. Its biosynthesis and metabolic pathways are largely unknown. However, the widespread occurrence of the toxin in phylogenetically distinct marine organisms is consistent with its production by microorganisms and subsequent accumulation in the food chain. To investigate a possible microbial origin, bacteria from two zoanthid corals (Palythoa caribaeorum, Zoanthus pulchellus) and one sponge (Neofibularia nolitangere) were isolated. More than 250 bacteria were screened for hemolysis using a newly developed PTX-screening assay of which 7% showed PTX-like hemolytic activity. 16S rRNA gene sequencing revealed that these bacterial isolates belonged to strains of Bacillus cereus group (n = 11) as well as the genera Brevibacterium (n = 4) and Acinetobacter (n = 2). The results indicate the presence of Na+/K+-ATPase toxins and possibly PTX in hemolytic bacteria from P. caribaeorum.

Description: 18S ribosomal DNA and internal transcribed spacer 2 (ITS-2) full-length sequences, each of which was sequenced three times, were used to construct phylogenetic trees with alignments based on secondary structures, in order to elucidate genealogical relationships within the Aplysinidae (Verongida). The first poriferan ITS-2 secondary structures are reported. Altogether 11 Aplysina sponges and 3 additional sponges (Verongula gigantea, Aiolochroia crassa, Smenospongia aurea) from tropical and subtropical oceans were analyzed. Based on these molecular studies, S. aurea, which is currently affiliated with the Dictyoceratida, should be reclassified to the Verongida. Aplysina appears as monophyletic. A soft form of Aplysina lacunosa was separated from other Aplysina and stands at a basal position in both 18S and ITS-2 trees. Based on ITS-2 sequence information, the Aplysina sponges could be distinguished into a single Caribbean–Eastern Pacific cluster and a Mediterranean cluster. The species concept for Aplysina sponges as well as a phylogenetic history with a possibly Tethyan origin is discussed.

Description: In nature, multiple-species rather than single-species microbial associations with plant or animal (including human) hosts are the rule more than the exception. Prominent examples are the microbial consortia of vertebrate intestines and cattle rumen. As many demosponges are associated with enormous amounts of microorganisms, contributing up to 40-60% of the sponge biomass, they are excellent models for marine multi-species, microbe-host associations. Representatives of at least eight different phyla, many of which contain few or no cultivated representatives, have been identified as specific members of the sponge-associated microbiota. Recent studies show that vertical transmission of symbionts through the larval stages rather than horizontal acquisition from seawater appears to be an important mechanism by which the complex and possibly ancient microbial consortia of sponges are formed.

Description: Many sponge species contain large and diverse communities of microorganisms. Some of these microbes are suggested to be in a mutualistic interaction with their host sponges, but there is little evidence to support these hypotheses. Stable nitrogen isotope ratios of sponges in the Key Largo, Florida (USA) area grouped sponges into species with relatively low δ15N ratios and species with relatively high δ15N ratios. Using samples collected in June 2002 from Three Sisters Reef and Conch Reef in the Key Largo, Florida area, transmission electron microscopy (TEM) and denaturing gradient gel electrophoresis were performed on tissues of the sponges Ircinia felix and Aplysina cauliformis, which are in the low δ15N group, and on tissue of the sponge Niphates erecta, which is in the high δ15N group. Results showed that I. felix and A. cauliformis have large and diverse microbial communities, while N. erecta has a low biomass of one bacterial strain. As the low δ15N ratios indicated a microbial input of nitrogen, these results suggested that I. felix and A. cauliformis were receiving nitrogen from their associated microbial community, while N. erecta was obtaining nitrogen solely from external sources. Sequence analysis of the microbial communities showed a diversity of metabolic capabilities among the microbes of the low δ15N group, which are lacking in the high δ15N group, further supporting metabolic differences between the two groups. This research provides support for hypotheses of mutualisms between sponges and their associated microbial communities.