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  • INGV  (56)
  • American Chemical Society
  • American Institute of Physics (AIP)
  • Molecular Diversity Preservation International
  • 2020-2023  (71)
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  • 1
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    Differences in Leaf Chemistry and Glandular Trichome Density between Wild Southwestern American Hop ( Humulus neomexicanus ) and Commercial Hop Cultivars (2021)
    American Chemical Society
    In:  Journal of Agricultural and Food Chemistry, 69 (27). pp. 7798-7814.
    Details
    Publication Date: 2022-01-07
    Description: The female flowers (“cones”) of the hop plant (Humulus L.) produce compounds that contribute to the flavor and other properties of beer. Hop leaves and cones produce many of the same compounds, which also confer agronomic traits such as insect and disease resistance. Targeted and untargeted ultraperformance liquid chromatography-quadrupole time-of-flight–mass spectrometry with Waters MSE technology (UPLC-QTof-MSE) metabolomics were used to compare leaf phytochemical compositions of greenhouse-grown southwestern American wild Humulus neomexicanus (A. Nelson and Cockerell) Rydb. against a group of commercial hop cultivars consisting of both pure European Humulus lupulus L. and European–North American hybrids. Principal component analysis showed a clear distinction in chemical profiles between the two groups. H. neomexicanus leaves had a significantly higher content of total α acids (p = 4.4 × 10–9), total bitter acids (p = 2.6 × 10–6), cohumulone (p = 1.0 × 10–13), humulone + adhumulone (p = 9.1 × 10–4), and the prenylflavonoids xanthohumol (p = 0.013) and desmethylxanthohumol (p = 0.029) as well as significantly higher densities of glandular trichomes (p = 1.3 × 10–6), the biosynthetic site of those compounds. Most flavonol glycosides measured were also significantly more abundant in H. neomexicanus (p = 1.5 × 10–22 to 0.0027), whereas phenolic acids were consistently, but generally nonsignificantly (p 〉 0.05), more abundant in the cultivars. The higher bitter acid, prenylflavonoid, and flavonol glycoside content of H. neomexicanus leaves may help to confer more favorable insect and disease-resistance properties.
    Type: Article , PeerReviewed
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  • 2
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    Genetic and Biochemical Reconstitution of Bromoform Biosynthesis in Asparagopsis Lends Insights into Seaweed Reactive Oxygen Species Enzymology (2020)
    American Chemical Society
    In:  ACS Chemical Biology, 15 (6). pp. 1662-1670.
    Details
    Publication Date: 2022-01-07
    Description: Marine macroalgae, seaweeds, are exceptionally prolific producers of halogenated natural products. Biosynthesis of halogenated molecules in seaweeds is inextricably linked to reactive oxygen species (ROS) signaling as hydrogen peroxide serves as a substrate for haloperoxidase enzymes that participate in the construction these halogenated molecules. Here, using red macroalga Asparagopsis taxiformis, a prolific producer of the ozone depleting molecule bromoform, we provide the discovery and biochemical characterization of a ROS-producing NAD(P)H oxidase from seaweeds. This discovery was enabled by our sequencing of Asparagopsis genomes, in which we find the gene encoding the ROS-producing enzyme to be clustered with genes encoding bromoform-producing haloperoxidases. Biochemical reconstitution of haloperoxidase activities establishes that fatty acid biosynthesis can provide viable hydrocarbon substrates for bromoform production. The ROS production haloperoxidase enzymology that we describe here advances seaweed biology and biochemistry by providing the molecular basis for decades worth of physiological observations in ROS and halogenated natural product biosyntheses.
    Type: Article , PeerReviewed
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  • 3
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    Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics (2021)
    American Chemical Society
    In:  Chemical Reviews, 121 (9). pp. 5193-5239.
    Details
    Publication Date: 2022-01-07
    Description: Iron is an indispensable metabolic cofactor in both pro- and eukaryotes, which engenders a natural competition for the metal between bacterial pathogens and their human or animal hosts. Bacteria secrete siderophores that extract Fe3+ from tissues, fluids, cells, and proteins; the ligand gated porins of the Gram-negative bacterial outer membrane actively acquire the resulting ferric siderophores, as well as other iron-containing molecules like heme. Conversely, eukaryotic hosts combat bacterial iron scavenging by sequestering Fe3+ in binding proteins and ferritin. The variety of iron uptake systems in Gram-negative bacterial pathogens illustrates a range of chemical and biochemical mechanisms that facilitate microbial pathogenesis. This document attempts to summarize and understand these processes, to guide discovery of immunological or chemical interventions that may thwart infectious disease.
    Type: Article , PeerReviewed
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  • 4
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    Mass Spectrometry-Based Metabolomics Reveals a Concurrent Action of Several Chemical Mechanisms in Arabidopsis-Fusarium oxysporum Compatible and Incompatible Interactions (2020)
    American Chemical Society
    In:  Journal of Agricultural and Food Chemistry, 68 (51). pp. 15335-15344.
    Details
    Publication Date: 2022-01-07
    Description: Fusarium oxysporum is a destructive root-infecting plant pathogen that causes significant yield losses in many economically important crop species. Hence, a deeper understanding of pathogen infection strategies is needed. With liquid chromatography-tandem mass spectrometry and gas chromatography-time of flight mass spectrometry platforms, we analyzed the metabolic changes in a time-course experiment with Arabidopsis accessions either resistant (Col-0) or susceptible (Ler-0) to isolates of Fusarium oxysporum forma specialis matthioli infection. We showed a concurrent effect of Fusarium-derived polyols and the mycotoxin beauvericin in the suppression of the immune response of susceptible hosts. A significant increase in oxidized glutathione in the resistant host was probably associated with effective reactive oxygen species-mediated resistance responses. Through a combination of targeted and untargeted metabolomics, we demonstrated the concurrent action of several Arabidopsis defense systems as well as the concurrent action of several virulence systems in the fungal attack of susceptible Arabidopsis.
    Type: Article , PeerReviewed
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  • 5
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    Synthase-Selective Exploration of a Tunicate Microbiome by Activity-Guided Single-Cell Genomics (2021)
    American Chemical Society
    In:  ACS Chemical Biology .
    Details
    Publication Date: 2022-01-07
    Description: While thousands of environmental metagenomes have been mined for the presence of novel biosynthetic gene clusters, such computational predictions do not provide evidence of their in vivo biosynthetic functionality. Using fluorescent in situ enzyme assay targeting carrier proteins common to polyketide (PKS) and nonribosomal peptide synthetases (NRPS), we applied fluorescence-activated cell sorting to tunicate microbiome to enrich for microbes with active secondary metabolic capabilities. Single-cell genomics uncovered the genetic basis for a wide biosynthetic diversity in the enzyme-active cells and revealed a member of marine Oceanospirillales harboring a novel NRPS gene cluster with high similarity to phylogenetically distant marine and terrestrial bacteria. Interestingly, this synthase belongs to a larger class of siderophore biosynthetic gene clusters commonly associated with pestilence and disease. This demonstrates activity-guided single-cell genomics as a tool to guide novel biosynthetic discovery.
    Type: Article , PeerReviewed
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  • 6
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    Telerilevamento di vibrazioni delle strutture tramite telesensore infrasonico (2022)
    INGV
    Details
    Publication Date: 2022-04-06
    Description: Nell’ambito del progetto EDISECUR, finanziato della regione Lazio, è stato sviluppato un prototipo di telesensore infrasonico, TIS, a tracciamento di speckle per la misura della velocità angolare, delle frequenze fondamentali e delle armoniche di una superficie sottoposta a oscillazioni. Il TIS si presta particolarmente per il rilevamento e il monitoraggio nel tempo degli edifici, ponti e altri manufatti. Conoscere lo stato vibrazionale di queste strutture può essere d’interesse sia per la loro caratterizzazione dinamica che per la sicurezza. Questo prototipo, sufficientemente compatto, si presta a una misura immediata della velocità angolare e, con semplici operazioni, si possono dedurre spostamenti e accelerazioni angolari. Dalle grandezze angolari e dalla conoscenza geometrica della superficie, tramite alcuni schemi ed esempi, si mostra come sia possibile determinare anche altri parametri cinematici lineari. Il telesensore può essere impiegato nelle misure delle vibrazioni di superfici a lunga distanza di varia natura, anche laddove non fosse possibile accedere per eseguire una misura diretta. Lo strumento copre un campo di frequenze fino alle decine di Hz, ha una sensibilità e una dinamica tale da rilevare le vibrazioni indotte dal rumore industriale, dal traffico, dal vento e altro. Questo lavoro è principalmente rivolto alle applicazioni del TIS nel rilevamento delle vibrazioni delle strutture ed è in questo ambito che vengono spiegate le modalità, i limiti e i vantaggi del suo impiego insieme agli errori insiti nella tecnica di misura. Dato che il TIS misura un movimento relativo tra lo stesso strumento e la superficiebersaglio, sono stati valutati gli errori delle vibrazioni dovute alla microsismicità e altre cause ambientali. Vengono infine riportati due preliminari esempi di misura su una struttura edile.
    Description: Regione Lazio, progetto EDISECUR
    Description: Published
    Description: 1-36
    Description: 7TM.Sviluppo e Trasferimento Tecnologico
    Description: JCR Journal
    Keywords: Remote Sensor ; Vibration Detector
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 7
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    Bollettino Sismico Italiano 2015 (2022)
    INGV
    Details
    Publication Date: 2022-04-11
    Description: L’Istituto Nazionale di Geofisica e Vulcanologia (INGV) riceve, nella Sala di Sorveglianza Sismica e Centro Allerta Tsunami di Roma, i segnali in tempo reale da centinaia di stazioni sismiche distribuite sul territorio nazionale. Entro due minuti dall’occorrenza di un qualsiasi terremoto, appositi sistemi automatici forniscono una prima valutazione dei parametri ipocentrali. Due sismologi, sempre presenti nella sala operativa della sede centrale, controllano le informazioni ottenute e, per i terremoti sopra una determinata soglia di magnitudo (ML ≥ 2.5), comunicano alla Sala Situazione Italia della Protezione Civile i dati elaborati, in media in circa 12 minuti (massimo entro 30 minuti) [Margheriti et al., 2021]. La valutazione definitiva dei parametri ipocentrali di tutti i terremoti, dai più grandi avvertiti in vaste aree del territorio ai più piccoli rilevati solo da pochi strumenti, è demandata a un’analisi più accurata svolta in un secondo tempo, ormai da alcuni decenni, da un gruppo di analisti specializzati nell’interpretazione dei segnali sismici. Gli analisti sismologi del Bollettino Sismico Italiano revisionano tutti i dati registrati dalle stazioni della Rete Sismica Nazionale (RSN) dell’INGV e riconoscono la presenza di terremoti attraverso un’analisi diretta delle forme d’onda. In tal modo l’analista rileva il tempo d’arrivo delle onde sismiche ai vari sensori e valuta l’ampiezza delle oscillazioni e la direzione del moto del suolo; questi parametri, utilizzati da apposite procedure di calcolo, consentono di localizzare ogni terremoto e di valutare la magnitudo associata. Le informazioni così ottenute confluiscono nel database che l’INGV gestisce e che mette a disposizione della comunità1. Questa pubblicazione ha come scopo quello di far conoscere un prodotto dell’Istituto Nazionale di Geofisica e Vulcanologia, Il Bollettino Sismico Italiano (BSI), con particolare riferimento all’anno 2015. Saranno delineate le principali caratteristiche della sismicità naturale e quella di origine antropica registrata in Italia nel corso dell’anno esaminato.
    Description: Published
    Description: 1-48
    Description: 4IT. Banche dati
    Description: JCR Journal
    Keywords: Bollettino Sismico Italiano 2015 ; Italian Seismic Bulletin 2015 ; sequences and seismic swarms ; explosion ; 04. Solid Earth::04.06. Seismology
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 8
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    Geomorphology and Prehistoric Settlements on a Volcanic Island: the Case of Ustica (Palermo, Italy) (2022)
    INGV
    Details
    Publication Date: 2022-03-07
    Description: This study represents the first attempt to combine the geomorphological characteristics of the island of Ustica with the human settlements that have been established during prehistory, with the purpose of reconstructing the interactions between communities and the natural environment from the Neolithic to the Middle Bronze Age (6th - 1st millennia B.C.). Ustica is a small island in the Southern Tyrrhenian Sea, visible but far (~55 km) from the northern coast of western Sicily. Its rugged volcanic nature, remodeled and enriched by the sea, offered to the first colonizers a wide repertoire of opportunities and challenges. This island can be treated as an ideal “laboratory” to understand how settlers, taking their first steps towards the foundation of organized communities, were able to seize opportunities or succumb to obstacles. The review of archaeological research until now carried out in Ustica, integrated with geomorphological data and other biogeographical indicators, offers a picture of the prehistory of Ustica in which human presence is continuous and distributed in various sites of the island characterized by different physiographic characteristics. There are phases dominated by the choice of naturally protected sites and phases in which settlements expands on open land, suitable for agricultural use. Where the archaeological evidence is scarce, the geomorphological peculiarities allow us to decipher the vocations and characters of a human settlement. The study leads to an open question: in the Middle Bronze Age, after about five thousand years of uninterrupted habitation of Ustica, which factors, geological, social, or other, induced the early communities to abandon the island, without returning there for about eight centuries, until the Hellenistic-Roman age?
    Description: Published
    Description: VO550
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Keywords: Geoarchaeology ; Geoarchaeology ; Prehistoric Settlements ; Island Archaeology ; Volcanic Landscape
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 9
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    A study for the selection of a calibration system for seismic sensors (2022)
    INGV
    Details
    Publication Date: 2022-03-09
    Description: This work sets out to identify a state-of-the-art system to be used for the calibration of seismic sensors. The aim is to acquire such a system within the framework of the PON ARS01 00926 EWAS (an Early Warning System for cultural heritage) project, which seeks to develop new technologies for the protection, conservation and safety of cultural heritage and envisages creating a newly developed seismic monitoring system. This system will exploit the ETL3D/5s-H hybrid sensors, resulting from the integration of a precision accelerometer within the ETL3D/5s velocimeter [Fertitta et al., 2020]. The new calibration system, already acquired and being installed, can be used by the EWAS project partners (including the National Institute of Geophysics and Volcanology and the Kore University of Enna), to calibrate the ETL3D/5s-H sensors, and by external organisations to calibrate or gauge other seismic sensors, thus providing a useful service to the scientific community and supporting industrial activities. This paper presents the method used and the activities undertaken to define the technical specifications of the calibration system. A feasibility study of an electromechanical vibrating table and the testing of two electrodynamic calibration systems were carried out. One of the electrodynamic systems is the CS18P (Calibration System for Seismic Sensors) produced by the German firm SPEKTRA. The CS18P comprises two vibrating tables, one horizontal and one vertical, which, thanks to their fluid-dynamic suspension, eliminate the sliding and rolling friction associated with the movement of the moving part with respect to the fixed part. A hardware and software system monitors and controls the motion in real time, analyses the data and automatically processes a predefined set of measurements. In the light of the technical specifications and experimental results, the CS18P represents the ideal solution for the aims of the EWAS project and also in view of the possible future uses of the calibration system.
    Description: Published
    Description: 1-38
    Description: 2IT. Laboratori analitici e sperimentali
    Description: JCR Journal
    Keywords: Seismic sensors calibration, Vibration exciter, Seismometer
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 10
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    Earthquake Rupture Forecasts for the MPS19 Seismic Hazard Model of Italy (2021)
    INGV
    Details
    Publication Date: 2022-04-29
    Description: In recent years, new approaches for developing earthquake rupture forecasts (ERFs) have been proposed to be used as an input for probabilistic seismic hazard assessment (PSHA). Zone- based approaches with seismicity rates derived from earthquake catalogs are commonly used in many countries as the standard for national seismic hazard models. In Italy, a single zone- based ERF is currently the basis for the official seismic hazard model. In this contribution, we present eleven new ERFs, including five zone-based, two smoothed seismicity-based, two fault- based, and two geodetic-based, used for a new PSH model in Italy. The ERFs were tested against observed seismicity and were subject to an elicitation procedure by a panel of PSHA experts to verify the scientific robustness and consistency of the forecasts with respect to the observations. Tests and elicitation were finalized to weight the ERFs. The results show a good response to the new inputs to observed seismicity in the last few centuries. The entire approach was a first attempt to build a community-based set of ERFs for an Italian PSHA model. The project involved a large number of seismic hazard practitioners, with their knowledge and experience, and the development of different models to capture and explore a large range of epistemic uncertainties in building ERFs, and represents an important step forward for the new national seismic hazard model.
    Description: Published
    Description: SE220
    Description: 6T. Studi di pericolosità sismica e da maremoto
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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