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  • Springer  (25)
  • American Meteorological Society  (7)
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  • 2020-2023  (7)
  • 1995-1999  (25)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    OR spectrum 20 (1998), S. 237-250 
    ISSN: 1436-6304
    Keywords: Transport ; Container ; Containerbelade-problem ; Tabu Search ; Praxisrelevante Restriktionen ; Packing ; Container ; Container loading problem ; Tabu search ; Practical restrictions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Economics
    Description / Table of Contents: Abstract The paper presents a tabu search algorithm (TSA) for weakly heterogeneous container loading problems. The approach includes two integrated components: The generation of different stowage plans by means of a greedy algorithm and a tabu search using an appropriate representation of these plans. The TSA considers some restrictions of practical relevance. The overall algorithm has been applied to numerous benchmark problems and its performance is demonstrated by a numerical comparison with several loading procedures suggested by other authors.
    Notes: Zusammenfassung Gegenstand des Beitrags ist die Entwicklung eines Tabu Search-Verfahrens (TSV) für Containerbeladeprobleme mit schwach heterogenem Kistenvorrat. Das TSV basiert auf einem zweistufigen Verfahrensansatz. Ein integrierter Greedy-Algorithmus dient unmittelbar der Erzeugung von Stauplänen. Vermittelt über eine geeignete Repräsentation zulässiger Problemlösungen wird in einer zweiten Verfahrensstufe eine Tabu-Suche durchgeführt. Das TSV berücksichtigt einige praxisrelevante Restriktionen. Die Performance des TSV wird anhand von Benchmarkproblemen aus der Literatur demonstriert, wobei Containerbeladeverfahren verschiedener Autoren zu Vergleichszwecken herangezogen werden.
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  • 2
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    Springer
    Biology and fertility of soils 20 (1995), S. 63-69 
    ISSN: 1432-0789
    Keywords: Lumbricus rubellus ; Introduced bacteria ; Transport ; Survival ; Casts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four strains of bacteria, Rhizobium leguminosarum biovar trifolii, Pseudomonas fluorescens, Pseudomonas cepacia, and Flavobacterium sp., were introduced into loamy sand and then transported by earthworms of the species Lumbricus rubellus to uninoculated soil. Cell densities recovered from the earthworm gut and casts (both expressed per gram dry material) were significantly lower (up to 3 log units) than cell densities recovered from the inoculated soil. Total bacterial counts in casts were similar to those in the inoculated soil. In casts excreted into a sterile environment numbers of colony-forming units (CFU) increased, suggesting a favourable environment for growth. In casts excreted in a non-sterile environment, cell densities of introduced strains decreased. Casts therefore did not offer the introduced bacteria a protective micro-environment for survival in the bulk soil. Transport by worms of R. leguminosarum biovar trifolii and of P. fluorescens appeared to occur mostly by means of cast production; with the Flavobacterium sp. and P. cepacia a large proportion of the cells was possibly transported on the skin of earthworms.
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  • 3
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    Journal of biomedical science 3 (1996), S. 69-77 
    ISSN: 1423-0127
    Keywords: HIV ; Rev ; Nucleus ; Transport ; RNA ; Cofactors ; Processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The Rev axis of HIV is one of two key autoregulatory pathways required for viral replication and pathogenesis. The viral Rev protein interacts with its RNA target sequence, the RRE, to overcome the inhibitory effects of constitutive repressor sequences and promote nucleocytoplasmic transport and expression of viral RNAs. The Rev axis is the subject of intense scrutiny not only because it plays a central role in the viral life cycle, but also because it offers a window onto the workings of key mechanisms of posttranscriptional regulation, including splicing, polyadenylation, degradation, transport, and translation. Recent reports have conclusively demonstrated a central role for transport in the Rev mechanism and have identified cellular factors that are good candidates for mediating the transport phenomena. Other potentially involved cellular factors are being investigated. Much of the apparent heterogeneity in the observed effects of Rev may actually derive from heterogeneity in the constitutive repressor sequences rather than from heterogeneity in the mechanism of action of Rev per se.
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  • 4
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    Springer
    Environmental geology 27 (1996), S. 300-308 
    ISSN: 1432-0495
    Keywords: Subseabed disposal ; Consolidation ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Subseabed disposal of radioactive waste applies a multiple-barrier concept with the sediment being the most important barrier for preventing a release of nuclides into the biosphere. While many investigations have been carried out to analyze the risk potential in this type of disposal, the effects of sediment consolidation and associated fluid flow have not fully been taken into consideration. Here, possible effects of consolidational fluid flow in the penetrator disposal option and possible consequences to the transport of nuclides in the sediment are analyzed. Results of numerical experiments demonstrate that consolidation contributes to the transport of radioactive nuclides released from containers buried in the sediment and to the release of nuclides at the sediment-water interface. Both depend on geological conditions and to a large extent on possible alterations of hydraulic conductivity i of the sediment in the vicinity of the entry path of a penetrator.
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  • 5
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    Environmental geology 25 (1995), S. 71-85 
    ISSN: 1432-0495
    Keywords: Effective porosity ; Longitudinal dispersivity ; Tracer test ; Transport ; Groundwater flow ; Sedimentary rock ; Dispersion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract We performed two field tracer tests in Tertiary sedimentary rocks and laboratory tracer tests using core samples of the sedimentary rock and bentonite and quartz sand mixtures. We have estimated effective porosity and longitudinal dispersivity, which are essential to the evaluation of mass transport in groundwater. The results of these tests indicate that test scale should be considered in the evaluation of effective porosity and longitudinal dispersivity. As the test scale increases, effective porosity decreases and longitudinal dispersivity increases. The relationship may be useful for estimating mass transport in groundwater. From the laboratory scale to the field scale, the decrease in effective porosity and increase in longitudinal dispersivity may be due to the existence of cracks or fissures. At the laboratory scale, the decrease in effective porosity is probably due to a loss of connectivity of continuous pores.
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  • 6
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    Environmental geology 27 (1996), S. 300-308 
    ISSN: 1432-0495
    Keywords: Key words: Subseabed disposal ; Consolidation ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract. Subseabed disposal of radioactive waste applies a multiple-barrier concept with the sediment being the most important barrier for preventing a release of nuclides into the biosphere. While many investigations have been carried out to analyze the risk potential in this type of disposal, the effects of sediment consolidation and associated fluid flow have not fully been taken into consideration. Here, possible effects of consolidational fluid flow in the penetrator disposal option and possible consequences to the transport of nuclides in the sediment are analyzed. Results of numerical experiments demonstrate that consolodation contributes to the transport of radioactive nuclides released from containers buried in the sediment and to the release of nuclides at the sediment-water interface. Both depend on geological conditions and to a large extent on possible alterations of hydraulic conductivity of the sediment in the vicinity of the entry path of a penetrator.
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  • 7
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    European biophysics journal 26 (1997), S. 471-476 
    ISSN: 1432-1017
    Keywords: Key words Ion channel ; Transport ; Polymers ; Hydration ; Water structure ; Alpha hemolysin ; Poly(ethylene glycol)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Electrostatic potentials created by fixed or induced charges regulate many cellular phenomena including the rate of ion transport through proteinaceous ion channels. Nanometer-scale pores of these channels also play a critical role in the transport of charged and neutral macromolecules. We demonstrate here that, surprisingly, changing the charge state of a channel markedly alters the ability of nonelectrolyte polymers to enter the channel's pore. Specifically, we show that the partitioning of differently-sized linear nonelectrolyte polymers of ethylene glycol into the Staphylococcus aureus α-hemolysin channel is altered by the solution pH. Protonating some of the channel side chains decreases the characteristic polymer size (molecular weight) that can enter the pore by ∼25% but increases the ionic current by ∼15%. Thus, the “steric” and “electric” size of the channel change in opposite directions. The results suggest that effects due to polymer and channel hydration are crucial for polymer transport through such pores.
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  • 8
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    Archives of microbiology 165 (1996), S. 1-8 
    ISSN: 1432-072X
    Keywords: Key wordsEscherichia coli ; ATPase ; Energetics ; Membrane protein ; Protein folding ; Sec Proteins ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Translocation of precursor proteins across the cytoplasmic membrane in bacteria is mediated by a multi-subunit protein complex termed translocase, which consists of the integral membrane heterotrimer SecYEG and the peripheral homodimeric ATPase SecA. Preproteins are bound by the cytosolic molecular chaperone SecB and targeted in a complex with SecA to the translocation site at the cytoplasmic membrane. This interaction with SecYEG allows the SecA/preprotein complex to insert into the membrane by binding of ATP to the high affinity nucleotide binding site of SecA. At that stage, presumably recognition and proofreading of the signal sequence occurs. Hydrolysis of ATP causes the release of the preprotein in the translocation channel and drives the withdrawal of SecA from the membrane-integrated state. Hydrolysis of ATP at the low-affinity nucleotide binding site of SecA converts the protein into a compact conformational state and releases it from the membrane. In the absence of the proton motive force, SecA is able to complete the translocation stepwise by multiple nucleotide modulated cycles.
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  • 9
    ISSN: 1432-072X
    Keywords: Key words Thiamin ; Biosynthesis ; Prokaryotes ; Transport ; Kinase ; Mechanism ; Structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thiI, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and four are kinase genes (thiD, thiM, thiL, and pdxK). The specific reactions catalyzed by ThiEF, Dxs, ThiDM, ThiL, and PdxK have been reconstituted in vitro and ThiS thiocarboxylate has been identified as the sulfur source. The X-ray structures of thiamin phosphate synthase and 5-hydroxyethyl-4-methylthiazole kinase have been completed. The genes coding for the thiamin transport system (thiBPQ) have also been identified. Remaining problems include the cloning and characterization of thiK (thiamin kinase) and the gene(s) involved in the regulation of thiamin biosynthesis. The specific reactions catalyzed by ThiC (pyrimidine formation), and ThiGH and ThiI (thiazole formation) have not yet been identified.
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  • 10
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    JBIC 4 (1999), S. 523-527 
    ISSN: 1432-1327
    Keywords: Key words Magnesium ; Transport ; Prokaryotes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  Possessing the largest hydrated radius, the smallest ionic radius, and the highest charge density among the biologically relevant cations, Mg2+ provides an interesting problem for transport into living cells. Transport systems for Mg2+ have been characterized primarily in Salmonella typhimurium because the well-developed genetics of Gram-negative bacteria make cloning and studying the transporters a viable proposition. The CorA transport system is expressed constitutively and is the major Mg2+ transporter in Eubacteria and Archaea. It has three transmembrane domains, a uniquely large periplasmic domain, and no sequence homology to other proteins. The MgtE Mg2+ transporter also lacks sequence homology to other proteins, and it is unclear if Mg2+ transport is its primary function. The MgtA and MgtB Mg2+ transporters have sequence homology to P-type ATPases. They are more closely related to the mammalian Ca2+–ATPases than to the prokaryotic P-type ATPases. MgtA and MgtB mediate Mg2+ influx with, rather than against, the Mg2+ electrochemical gradient. Unlike corA and mgtE, the mgtA and mgtC/mgtB loci are regulated, being induced by the two-component regulatory system PhoP/PhoQ. PhoQ is a Mg2+ membrane sensor kinase that phosphorylates the transcription factor PhoP under Mg2+–limiting conditions. This factor then induces transcription of mgtA and mgtCB. MgtC, which is encoded by the first gene in the mgtCB operon, has no sequence homology to any known protein and is essential for S. typhimurium virulence in mice and macrophages, but does appear to be a Mg2+ transporter. The physiological roles of these Mg2+ transporters and their mechanisms are not yet completely clear, but initial data indicate that Mg2+ transporters are unique transport systems with unusual mechanisms for mediating Mg2+ movement through the membrane.
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  • 11
    ISSN: 1432-1327
    Keywords: Key words Lanthanide ; Erythrocyte ; Transport ; Confocal laser scanning microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  A novel method has been developed to visualize and follow the temporal course of lanthanide transport across the membrane into a single living erythrocyte. By means of confocal scanning microscopy and the optical section technique, the entry of lanthanide ions was followed by the fluorescence quenching of fluorescein isothiocyanate (FITC)-labeled membrane and cytosol. From the difference of the quenching kinetics of the whole section and the central area, the time for diffusion through the membrane and the diffusion in the extracellular and intracellular media can be deduced. To clarify the mechanism of lanthanide-induced fluorescence quenching of FITC-labeled erythrocytes and to ensure that this reaction can be used in this method, the reaction was investigated by steady-state fluorescence techniques. The results showed that the lanthanides strongly quenched the florescence emitted by FITC covalently bound to membrane proteins and cytosolic proteins. The static quenching mechanism is responsible for the fluorescence quenching of FITC-labeled proteins by Ln species. The quenching mechanism is discussed on the basis of complex formation. The dependence of fluorescence quenching on both ion size and the total orbital angular momentum L supports the complexation mechanism. The transport time across the membrane is strikingly correlated with Ln species and extracellular concentration. For a given concentration, the transport time of [Ln(cit)2]3– is much shorter than that of Ln3+, since they enter the cells via the anion channel. This is supported by the inhibition effect of 4,4′-diisothiocyanato-2,2′-stilbenendisulfonate on the transport of [Ln(cit)2]3–. On the other hand, the transport of free Ln3+ might be attributed to the enhanced permeability of erythrocytes owing to Ln3+ binding. These findings strongly demonstrate the existence of the non-internalization mechanism of Ln species uptake by erythrocytes.
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  • 12
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    Journal of computer-aided materials design 6 (1999), S. 283-309 
    ISSN: 1573-4900
    Keywords: Aluminum ; Growth ; Nucleation ; Predictive process engineering ; Reaction ; Semiconductor processing ; Thin films ; Transport ; Tungsten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Two case studies are presented in order to highlight the status of materials modeling in semiconductor materials processing, as well as some opportunities in the area. Both case studies involve programmed rate chemical vapor deposition (PRCVD), which is a CVD process in which conditions are systematically changed during deposition in order to enhance either processing properties or resulting film properties. In the tungsten study, quantitative simulations, based on fundamental transport and reaction modeling and a continuum film representation, are used to guide experiments that demonstrate how PRCVD can provide significantly greater throughput than conventional, constant rate CVD (CRCVD). We start the deposition process at a much higher temperature, compared to a CRCVD process, then decrease the temperature during deposition. We achieve throughput increases of about a factor of three, with more improvement clearly obtainable. In addition to the increase in throughput, the properties of the PRCVD films are equal to, or superior to, CRCVD films. The aluminum PRCVD case study demonstrates some opportunities for materials modeling. The protocols used are based upon qualitative models of nucleation and film growth, as there are no simulators that predict microstructure and film properties. Nevertheless, we demonstrate that the PRCVD processes, designed using qualitative models, can yield films with better properties than CRCVD processes. PRCVD films can have higher nuclei densities, higher fractions of (111) orientated Al, lower surface roughnesses, higher reflectivities, and resistivities closer to that of bulk aluminum. In general, PRCVD protocols provide degrees of freedom that can be used to improve processing or film properties. PRCVD may become much more important as films get thinner and interfaces become more important.
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  • 13
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    Journal of computer-aided materials design 3 (1996), S. 385-402 
    ISSN: 1573-4900
    Keywords: Lithium ; Polyethylene oxide ; Batteries ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary We have made a molecular dynamics study of transport of a single lithium ion in a previously reported model of amorphous polyethylene oxide. New ab initio calculations of the interaction of the lithium ion with 1,2-dimethoxyethane and with dimethyl ether are reported which are used to determine force fields for the simulation. We report preliminary calculations of solvation energies and hopping barriers and a calculation of the ionic conductivity which is independent of any assumptions about the mechanism of ion transport. We also report some details of a study of transport of the trapped lithium ion on intermediate time and length scales.
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  • 14
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    Planta 204 (1998), S. 131-139 
    ISSN: 1432-2048
    Keywords: Key words: Aquaporin ; Plasma membrane intrinsic protein ; Tonoplast intrinsic protein ; Transport ; Water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 15
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    Cell & tissue research 292 (1998), S. 219-227 
    ISSN: 1432-0878
    Keywords: Key words Photoreceptor cells ; Transport ; intracellular ; Cytoskeleton ; Myosin ; Actin filaments ; Mitochondria ; Schistocerca gregaria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Light stimulation of locust (Schistocerca gregaria) photoreceptors results in an actin-dependent translocation of mitochondria towards the photoreceptive microvilli and an antagonistic movement of endoplasmic reticulum towards the cell body. Using immunocytochemical techniques, we have tried to identify myosin-like motors that may drive the light-induced organelle motility. A monoclonal antibody against the motor domain of Acanthamoeba myosin identifies a prominent 110-kDa protein on Western blots of locust retina. Cross-reactivity with two polyclonal anti-myosin antibodies and a monoclonal anti-myosin-I-antibody, together with ATP-dependent binding to actin filaments, provides evidence that the 110-kDa protein is an unconventional myosin. By indirect immunofluorescence, the 110-kDa protein has been localized to both photoreceptors and pigment cells within the retina. In the photoreceptor cells, the 110-kDa protein is bound to the surface of mitochondria. This putative unconventional myosin may thus be a motor protein involved in the light-induced translocation of mitochondria in photoreceptors.
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  • 16
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    Cell & tissue research 286 (1996), S. 547-549 
    ISSN: 1432-0878
    Keywords: Key words: Compound eye ; Photoreceptor cells ; Cytoskeleton ; Transport ; intracellular ; Microtubules ; Membrane turnover ; Schistocerca gregaria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Light stimulation of locust photoreceptors causes a translocation of submicrovillar cisternae of the endoplasmic reticulum away from the rhabdome, and a movement of mitochondria towards the rhabdome. To examine whether the microtubule cytoskeleton could be involved in these organelle movements, we have analysed the distribution of the microtubule-dependent motor proteins kinesin and cytoplasmic dynein in the retina of the locust Schistocerca gregaria. Both kinesin and cytoplasmic dynein are associated with vesicular structures that are distinct from mitochondria and the submicrovillar endoplasmic reticulum. These results, together with the previous demonstration of a lack of microtubules in the cell area of light-dependent organelle movements, provide evidence that the microtubule cytoskeleton is not involved with light-induced organelle translocations.
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  • 17
    ISSN: 1432-0878
    Keywords: Key words: Intestine ; Endocytosis ; Tight junctions ; Carbachol ; Forskolin ; Transport ; Carassius auratus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The transepithelial route for mucosa-to-serosa transport of the tracer macromolecule horseradish peroxidase (HRP; MW 40 kDa) and modulation of this transport by forskolin and carbachol have been studied in vi-tro in stripped goldfish intestinal epithelium mounted in Ussing-type chambers. Uptake and transport have been investigated by measuring the HRP flux from the muco-sal to serosal sides by an enzymatic method and by visualising HRP reaction products in the mucosa with electron-microscopical techniques. Both the cholinergic agonist carbachol (which is thought to increase intracellular Ca2+ and activate protein kinase C activity) and forskolin (a direct activator of adenylylcyclase) affect the amount of enzymatically active HRP in the tissue. In control tissue, HRP product is found only within the epithelial cells, the transepithelial flux reaching a constant value of about 1.5 pmoles/cm2 per h. Carbachol increases the amount of HRP product in the cells, but has no significant effect on the HRP flux compared with control values. Forskolin decreases the amount of HRP product in the cells; however, in the presence of forskolin, the lateral intercellular spaces become filled with HRP product. HRP is found in the lamina propria and the transepithelial protein flux increases more than 2.5-fold. In the presence of forskolin plus carbachol, the results are no different from the control. It is concluded that carbachol increases the endocytotic uptake of HRP, whereas forskolin inhibits the uptake but increases the paracellular permeability for HRP in goldfish intestine.
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  • 18
    ISSN: 1432-0878
    Keywords: Key words: Arginine ; Analogues ; Immunocytochemistry ; Nitric oxide ; Pituitary ; Retina ; Transport ; Rat (Wistar) ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Nitric oxide is formed by the action of nitric oxide synthase upon l-arginine. The efficacy of some exogenously applied arginine analogues in inhibiting nitric oxide synthase and thus nitrergic transmission indicates that neurons producing nitric oxide may possess an arginine transport system. To investigate whether arginine analogues are preferentially transported into nitric oxide-utilising cells or into cells making other neurochemicals, we have raised highly specific antisera against a number of arginine analogues including NG-methyl arginine, d-arginine, NGnitro-l-arginine, NG-nitro-l-arginine methyl ester and canavanine. Retinae were incubated in physiological media containing these analogues and rats were given intraperitoneal injections of the analogues to study the pituitary. Immunocytochemistry and NADPH-diaphorase histochemistry revealed that many of these analogues could be transported preferentially, but not exclusively, into nitric oxide-generating cells. However, some nitric oxide-producing cells apparently lacked the ability to take up some arginine analogues. We conclude that nitric oxide-generating cells in the retina and pituitary possess one or more arginine transporters. Other subsets of neurons that use GABA or glutamate as a neurotransmitter may also accumulate arginine analogues, possibly as a substrate for formation of these neurochemicals.
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  • 19
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    Bioscience reports 15 (1995), S. 419-426 
    ISSN: 1573-4935
    Keywords: Transport ; glucose ; membrane trafficking ; interleukin-3 ; Abelson tyrosine kinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The uptake of glucose into mammalian cells, catalysed by members of the GLUT family of glucose transporters, is regulated by a variety of hormones, growth factors and other agents. In adipocytes, skeletal muscle and heart the principal regulator is the hormone insulin, which rapidly stimulates glucose uptake by bringing about the translocation of the GLUT4 glucose transporter isoform from an intracellular vesicular compartment to the cell surface. Recent studies have implicated theC-terminal hydrophilic region of this protein as being primarily responsible for its insulin-regulated trafficking. In an attempt to identify the protein machinery involved in this trafficking, we have used glutathione S-transferase fusion proteins bearing hydrophilic domains of various GLUT transporters in affinity purification experiments on detergent-solubilized extracts of 3T3-L1 adipocyte intracellular membranes. TheC-terminal region of GLUT4 was found specifically to bind a number of polypeptides in these extracts, which are therefore candidates for components of the trafficking machinery. Although these proteins did not bind to the corresponding region of the more widely-distributed GLUT1 glucose transporter isoform, regulation of this transporter also appears to be of physiological importance in some cell types. To study such regulation we have used as a model system the interleukin-3 (IL-3)-dependent haemopoietic cell line IC.DP. These cells express a temperature-sensitive mutane of thev-abl tyrosine kinase, whose activation at the permissive temperature permits cell survival in the absence of IL-3 by suppression of apoptosis, although the growth factor is still required for proliferation. Both IL-3 and activation of the kinase were found to stimulate glucose transport by promoting the translocation of GLUT1 to the cell surface. Moreover, inhibition of glucose uptake by addition of transport inhibitors markedly increased the rate of apoptosis, an effect which could be reversed by the provision of alternative energy sources. These observations suggest that the trafficking of GLUT1, regulated by growth factors or oncogenes, may play an important role in the suppression of apoptosis in haemopoietic cells.
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  • 20
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    Molecular genetics and genomics 259 (1998), S. 541-548 
    ISSN: 1617-4623
    Keywords: Key words Manganese ; Divalent cations ; Transport ; HIP1 ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a search for components involved in Mn2+ homeostasis in the budding yeast Saccharomyces cerevisiae, we isolated a mutant with modifications in Mn2+ transport. The mutation was found to be located in HIP1, a gene known to encode a high-affinity permease for histidine. The mutation, designated hip1–272, caused a frameshift that resulted in a stop codon at position 816 of the 1812-bp ORF. This mutation led to Mn2+ resistance, whereas the corresponding null mutation did not. Both hip1–272 cells and the null mutant exhibited low tolerance to divalent cations such as Co2+, Ni2+, Zn2+, and Cu2+. The Mn2+ phenotype was not influenced by supplementary histidine in either mutant, whereas the sensitivity to other divalent cations was alleviated by the addition of histidine. The cellular Mn2+ content of the hip1–272 mutant was lower than that of wild type or null mutant, due to increased rates of Mn2+ efflux. We propose that Hip1p is involved in Mn2+ transport, carrying out a function related to Mn2+ export.
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  • 21
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    Space science reviews 80 (1997), S. 49-76 
    ISSN: 1572-9672
    Keywords: Magnetosphere ; Ionosphere ; Plasma ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this review, we examine four specific questions/issues of contemporary interest within the overall topic of ionospheric plasma outflow into the magnetosphere. These four question areas are framed here as: (1) Are high-latitude F-region upflows predominantly driven by soft auroral electron precipitation?; (2) Is the O+ within the polar cap magnetosphere supplied primarily by the cleft ion fountain or a direct polar cap ionospheric source?; (3) Is centrifugal acceleration an important mechanism in the acceleration of ionospheric plasma outflows?; and (4) Are lobe ionospheric outflows captured by the plasma sheet?
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  • 22
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    Space science reviews 80 (1997), S. 217-234 
    ISSN: 1572-9672
    Keywords: Magnetopause ; Boundary Layer ; Magnetic Reconnection ; Diffusion ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract There is a long history of particle measurements that have established that plasma is being transferred across Earth's magnetopause in both directions. The paper reviews the nature of the observational evidence as well as the implications regarding the transfer mechanisms and their efficiencies.
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  • 23
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    Journal of biological physics 23 (1997), S. 111-120 
    ISSN: 1573-0689
    Keywords: Membrane ; Solution ; Transport ; Reverse osmosis ; Transport equations.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The work is concerned with an analytical description of reverseosmosis using the Kedem-Katchalsky equations.The process has been considered for well-mixed solutions.We have obtained formulas describing the concentration of a solution(purified by reverse osmosis) as a function of the transportparameters of a membrane, concentration of the initial solution, andthe mechanical pressures on the membrane.The formulae are illustrated by numerical computations.Our results may be applicable to biophysical studies concerningreverse osmosis in biological membrane systems.
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  • 24
    Electronic Resource
    Electronic Resource
    Springer
    Journal of biological physics 23 (1997), S. 233-238 
    ISSN: 1573-0689
    Keywords: Membrane ; Transport ; Transport parameters ; Correlation ; Correlation constant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract We derive a formula for the correlation of the three practical transportparameters Lp, σ, and ω appearing in Kedem-Katchalskyequations. It has a form ω = KLp/vs(1-σ), where K = 0.0306 is a universal constant independent ofthe choice of a membrane and a solute. It can be used to calculate the valueof any of these parameters, provided the other two and the molar volume $$\overline {\upsilon _s } $$ of the solute are known. The formula couldbe very useful, in particular when measurements of the parameters aredifficult or even impossible.
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  • 25
    Electronic Resource
    Electronic Resource
    Springer
    Journal of inclusion phenomena and macrocyclic chemistry 28 (1997), S. 105-116 
    ISSN: 1573-1111
    Keywords: Transport ; tetramers ; neutral arenes ; aqueous barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The transportation of neutral arenes such as benzene, toluene, o,m,p-xylene, durene,biphenyl, anthracene and phenanthrene through an aqueous medium using the crownconformation of tetrameric cyclophane hosts 1 and 2 has been studied. In general,transportation of all arenes increases with time, exceptfor toluene, which showssaturation behaviour with both hosts 1 and 2.Among the larger arenes, transportationof biphenyl is a maximum with 1; with host 2,transportation of anthracene is a maximumdue to its larger association constant. In the case ofsmaller arenes, 1 transportsbenzene most effectively, and transportation decreases asthe number of methyl groups onbenzene increases, showing that stereoelectronic factors affecttransportation. With 2toluene is transported most effectively due tothe change in the shape of the binding cavitycaused by a change in bridging groups. A comparison oftransportation for 1 h with 1 and 2shows that for all arenes transportation is greater withhost 2 than with 1 due to thehydrophobic nature of 2.
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  • 26
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(4), (2020): 887-905, doi:10.1175/JPO-D-19-0110.1.
    Description: The Equatorial Undercurrent (EUC) encounters the Galápagos Archipelago on the equator as it flows eastward across the Pacific. The impact of the Galápagos Archipelago on the EUC in the eastern equatorial Pacific remains largely unknown. In this study, the path of the EUC as it reaches the Galápagos Archipelago is measured directly using high-resolution observations obtained by autonomous underwater gliders. Gliders were deployed along three lines that define a closed region with the Galápagos Archipelago as the eastern boundary and 93°W from 2°S to 2°N as the western boundary. Twelve transects were simultaneously occupied along the three lines during 52 days in April–May 2016. Analysis of individual glider transects and average sections along each line show that the EUC splits around the Galápagos Archipelago. Velocity normal to the transects is used to estimate net horizontal volume transport into the volume. Downward integration of the net horizontal transport profile provides an estimate of the time- and areal-averaged vertical velocity profile over the 52-day time period. Local maxima in vertical velocity occur at depths of 25 and 280 m with magnitudes of (1.7 ± 0.6) × 10−5 m s−1 and (8.0 ± 1.6) × 10−5 m s−1, respectively. Volume transport as a function of salinity indicates that water crossing 93°W south (north) of 0.4°S tends to flow around the south (north) side of the Galápagos Archipelago. Comparisons are made between previous observational and modeling studies with differences attributed to effects of the strong 2015/16 El Niño event, the annual cycle of local winds, and varying longitudes between studies of the equatorial Pacific.
    Description: This work was supported by National Science Foundation (Grants OCE-1232971 and OCE-1233282) and the NASA Earth and Space Science Fellowship Program (Grant 80NSSC17K0443).
    Keywords: Tropics ; Boundary currents ; Topographic effects ; Transport ; Upwelling/downwelling ; In situ oceanic observations
    Repository Name: Woods Hole Open Access Server
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  • 27
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(10), (2020): 2849-2871, https://doi.org/10.1175/JPO-D-20-0086.1.
    Description: The structure, transport, and seasonal variability of the West Greenland boundary current system near Cape Farewell are investigated using a high-resolution mooring array deployed from 2014 to 2018. The boundary current system is comprised of three components: the West Greenland Coastal Current, which advects cold and fresh Upper Polar Water (UPW); the West Greenland Current, which transports warm and salty Irminger Water (IW) along the upper slope and UPW at the surface; and the Deep Western Boundary Current, which advects dense overflow waters. Labrador Sea Water (LSW) is prevalent at the seaward side of the array within an offshore recirculation gyre and at the base of the West Greenland Current. The 4-yr mean transport of the full boundary current system is 31.1 ± 7.4 Sv (1 Sv ≡ 106 m3 s−1), with no clear seasonal signal. However, the individual water mass components exhibit seasonal cycles in hydrographic properties and transport. LSW penetrates the boundary current locally, through entrainment/mixing from the adjacent recirculation gyre, and also enters the current upstream in the Irminger Sea. IW is modified through air–sea interaction during winter along the length of its trajectory around the Irminger Sea, which converts some of the water to LSW. This, together with the seasonal increase in LSW entering the current, results in an anticorrelation in transport between these two water masses. The seasonality in UPW transport can be explained by remote wind forcing and subsequent adjustment via coastal trapped waves. Our results provide the first quantitatively robust observational description of the boundary current in the eastern Labrador Sea.
    Description: A.P., R.S.P., F.B., D.J.T., and A.L.R. were funded by Grants OCE-1259618 and OCE-1756361 from the National Science Foundation. I.L.B, F.S., and J.H. were supported by U.S. National Science Foundation Grants OCE-1258823 and OCE-1756272. Mooring data from MA2 was funded by the European Union 7th Framework Programme (FP7 2007-2013) under Grant 308299 (NACLIM) and the Horizon 2020 research and innovation program under Grant 727852 (Blue-Action). J.K. and M.O. acknowledge EU Horizon 2020 funding Grants 727852 (Blue-action) and 862626 (EuroSea) and from the German Ministry of Research and Education (RACE Program). G.W.K.M. acknowledges funding from the Natural Sciences and Engineering Research Council.
    Keywords: Boundary currents ; Convection ; Deep convection ; Transport ; In situ oceanic observations ; Seasonal cycle
    Repository Name: Woods Hole Open Access Server
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  • 28
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    Unknown
    American Meteorological Society
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(11),(2020): 3331–3351, https://doi.org/10.1175/JPO-D-20-0035.1.
    Description: This study examines the generation of warm spiral structures (referred to as spiral streamers here) over Gulf Stream warm-core rings. Satellite sea surface temperature imagery shows spiral streamers forming after warmer water from the Gulf Stream or newly formed warm-core rings impinges onto old warm-core rings and then intrudes into the old rings. Field measurements in April 2018 capture the vertical structure of a warm spiral streamer as a shallow lens of low-density water winding over an old ring. Observations also show subduction on both sides of the spiral streamer, which carries surface waters downward. Idealized numerical model simulations initialized with observed water-mass densities reproduce spiral streamers over warm-core rings and reveal that their formation is a nonlinear submesoscale process forced by mesoscale dynamics. The negative density anomaly of the intruding water causes a density front at the interface between the intruding water and surface ring water, which, through thermal wind balance, drives a local anticyclonic flow. The pressure gradient and momentum advection of the local interfacial flow push the intruding water toward the ring center. The large-scale anticyclonic flow of the ring and the radial motion of the intruding water together form the spiral streamer. The observed subduction on both sides of the spiral streamer is part of the secondary cross-streamer circulation resulting from frontogenesis on the stretching streamer edges. The surface divergence of the secondary circulation pushes the side edges of the streamer away from each other, widens the warm spiral on the surface, and thus enhances its surface signal.
    Description: Authors W. G. Zhang and D. J. McGillicuddy are both supported by the National Science Foundation through Grant OCE 1657803.
    Keywords: Buoyancy ; Eddies ; Frontogenesis/frontolysis ; Mesoscale processes ; Transport ; Vertical motion
    Repository Name: Woods Hole Open Access Server
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  • 29
    Publication Date: 2022-05-27
    Description: Author Posting. © American Meteorological Society, 2021. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 51(7), (2021): 2087–2102, https://doi.org/10.1175/JPO-D-20-0255.1.
    Description: The boundary current system in the Labrador Sea plays an integral role in modulating convection in the interior basin. Four years of mooring data from the eastern Labrador Sea reveal persistent mesoscale variability in the West Greenland boundary current. Between 2014 and 2018, 197 middepth intensified cyclones were identified that passed the array near the 2000-m isobath. In this study, we quantify these features and show that they are the downstream manifestation of Denmark Strait Overflow Water (DSOW) cyclones. A composite cyclone is constructed revealing an average radius of 9 km, maximum azimuthal speed of 24 cm s−1, and a core propagation velocity of 27 cm s−1. The core propagation velocity is significantly smaller than upstream near Denmark Strait, allowing them to trap more water. The cyclones transport a 200-m-thick lens of dense water at the bottom of the water column and increase the transport of DSOW in the West Greenland boundary current by 17% relative to the background flow. Only a portion of the features generated at Denmark Strait make it to the Labrador Sea, implying that the remainder are shed into the interior Irminger Sea, are retroflected at Cape Farewell, or dissipate. A synoptic shipboard survey east of Cape Farewell, conducted in summer 2020, captured two of these features that shed further light on their structure and timing. This is the first time DSOW cyclones have been observed in the Labrador Sea—a discovery that could have important implications for interior stratification.
    Description: A. P. and R. S. P. were funded by National Science Foundation Grants OCE-1259618 and OCE-1756361. I. L. B. and F. S. were funded by National Science Foundation Grants OCE-1258823 and OCE-1756272. N. P. H. was supported by the Natural Environment Research Council U.K. OSNAP program (NE/K010875/1 and NE/K010700/1). M. A. S. was supported by NSF Grants OCE-1558742 and OPP-1822334.
    Description: 2021-12-08
    Keywords: Boundary currents ; Eddies ; Transport
    Repository Name: Woods Hole Open Access Server
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  • 30
    facet.materialart.
    Unknown
    American Meteorological Society
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(8), (2020): 2251-2270, doi:10.1175/JPO-D-19-0303.1.
    Description: The Gulf Stream affects global climate by transporting water and heat poleward. The current’s volume transport increases markedly along the U.S. East Coast. An extensive observing program using autonomous underwater gliders provides finescale, subsurface observations of hydrography and velocity spanning more than 15° of latitude along the path of the Gulf Stream, thereby filling a 1500-km-long gap between long-term transport measurements in the Florida Strait and downstream of Cape Hatteras. Here, the glider-based observations are combined with shipboard measurements along Line W near 68°W to provide a detailed picture of the along-stream transport increase. To account for the influences of Gulf Stream curvature and adjacent circulation (e.g., corotating eddies) on transport estimates, upper- and lower-bound transports are constructed for each cross–Gulf Stream transect. The upper-bound estimate for time-averaged volume transport above 1000 m is 32.9 ± 1.2 Sv (1 Sv ≡ 106 m3 s−1) in the Florida Strait, 57.3 ± 1.9 Sv at Cape Hatteras, and 75.6 ± 4.7 Sv at Line W. Corresponding lower-bound estimates are 32.3 ± 1.1 Sv in the Florida Strait, 54.5 ± 1.7 Sv at Cape Hatteras, and 69.9 ± 4.2 Sv at Line W. Using the temperature and salinity observations from gliders and Line W, waters are divided into seven classes to investigate the properties of waters that are transported by and entrained into the Gulf Stream. Most of the increase in overall Gulf Stream volume transport above 1000 m stems from the entrainment of subthermocline waters, including upper Labrador Sea Water and Eighteen Degree Water.
    Description: We gratefully acknowledge funding from the Office of Naval Research (N000141713040), the National Science Foundation (OCE-0220769, OCE-1633911, OCE-1923362), NOAA’s Global Ocean Monitoring and Observing Program (NA14OAR4320158, NA19OAR4320074), WHOI’s Oceans and Climate Change Institute, Eastman Chemical Company, and the W. Van Alan Clark, Jr. Chair for Excellence in Oceanography at WHOI (awarded to Breck Owens).
    Keywords: Continental shelf/slope ; North Atlantic Ocean ; Boundary currents ; Transport ; In situ oceanic observations ; Profilers, oceanic
    Repository Name: Woods Hole Open Access Server
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  • 31
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(7), (2020): 1839-1852, https://doi.org/10.1175/JPO-D-19-0209.1.
    Description: The Lagrangian characteristics of the surface flow field arising when an idealized, anticyclonic, mesoscale, isolated deep-ocean eddy collides with continental slope and shelf topography are explored. In addition to fluid parcel trajectories, we consider the trajectories of biological organisms that are able to navigate and swim, and for which shallow water is a destination. Of particular interest is the movement of organisms initially located in the offshore eddy, the manner in which the eddy influences the ability of the organisms to reach the shelf break, and the spatial and temporal distributions of organisms that do so. For nonswimmers or very slow swimmers, the organisms arrive at the shelf break in distinct pulses, with different pulses occurring at different locations along the shelf break. This phenomenon is closely related to the episodic formation of trailing vortices that are formed after the eddy collides with the continental slope, turns, and travels parallel to the coast. Analysis based on finite-time Lyapunov exponents reveals initial locations of all successful trajectories reaching the shoreline, and provides maps of the transport pathways showing that much of the cross-shelf-break transport occurs in the lee of the eddy as it moves parallel to the shore. The same analysis shows that the onshore transport is interrupted after a trailing vortex detaches. As the swimming speeds are increased, the organisms are influenced less by the eddy and tend to show up en mass and in a single pulse.
    Description: IR and LP were supported by National Science Foundation (NSF) Grant OCE-1558806. DC was supported by NSF U.S. National Science Foundation’s Physical Oceanography program through Grants OCE-1059632 and OCE-1433953 as well as the Academic Programs Office, Woods Hole Oceanographic Institution. We acknowledge high-performance computing support from Yellowstone (http://n2t.net/ark:/85065/d7wd3xhc) provided by NCAR’s Computational and Information Systems Laboratory, sponsored by the National Science Foundation.
    Keywords: Ocean ; Eddies ; Nonlinear dynamics ; Transport
    Repository Name: Woods Hole Open Access Server
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  • 32
    facet.materialart.
    Unknown
    American Meteorological Society
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2020. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 50(1), (2020): 255-268, doi:10.1175/JPO-D-19-0166.1.
    Description: Regional connectivity is important to the global climate salinity response, particularly because salinity anomalies do not have a damping feedback with atmospheric freshwater fluxes and may therefore be advected over long distances by ocean circulation, resulting in nonlocal influences. Climate model intercomparison experiments such as CMIP5 exhibit large uncertainty in some aspects of the salinity response, hypothesized here to be a result of ocean dynamics. We use two types of Lagrangian particle tracking experiments to investigate pathways of exchange for salinity anomalies. The first uses forward trajectories to estimate average transport time scales between water cycle regimes. The second uses reverse trajectories and a freshwater accumulation method to quantitatively identify remote influences in the salinity response. Additionally, we compare velocity fields with both resolved and parameterized eddies to understand the impact of eddy stirring on intergyre exchange. These experiments show that surface anomalies are readily exchanged within the ocean gyres by the mean circulation, but intergyre exchange is slower and largely eddy driven. These dynamics are used to analyze the North Atlantic salinity response to climate warming and water cycle intensification, where the system is broadly forced with fresh surface anomalies in the subpolar gyre and salty surface anomalies in the subtropical gyres. Under these competing forcings, strong intergyre eddy fluxes carry anomalously salty subtropical water into the subpolar gyre which balances out much of the local freshwater input.
    Description: We acknowledge the World Climate Research Programme’s Working Group on Coupled Modelling, which is responsible for CMIP, and we thank the climate modeling groups (listed in Table 1 of this paper) for producing and making available their model output. We also thank the creators of the SODA and ECCO reanalysis products. This work was supported by NASA Headquarters under the NASA Earth and Space Science Fellowship Program Award 80NSSC17K0372, and by National Science Foundation Award OCE-1433132. The SODA outputs used here can be accessed at http://www.atmos.umd.edu/~ocean/, and the ECCO outputs at https://ecco.jpl.nasa.gov/. Data from the CMIP5 ensemble is available at https://esgf-node.llnl.gov/projects/esgf-llnl/. The particle tracking code used for these experiments can be found at https://github.com/slevang/particle-tracking.
    Description: 2020-07-20
    Keywords: North Atlantic Ocean ; Eddies ; Hydrologic cycle ; Lagrangian circulation/transport ; Transport ; Climate change
    Repository Name: Woods Hole Open Access Server
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