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  • Articles  (17)
  • fungi  (17)
  • Springer  (17)
  • 2020-2023
  • 1995-1999  (17)
  • 1960-1964
  • 1945-1949
  • Process Engineering, Biotechnology, Nutrition Technology  (17)
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  • Articles  (17)
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  • 1
    Electronic Resource
    Electronic Resource
    Initial oxidative and subsequent conjugative metabolites produced during the metabolism of phenanthrene by fungi (1996)
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 205-215 
    Details
    ISSN: 1476-5535
    Keywords: phenanthrene ; fungi ; degradation ; conjugates ; metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Three filamentous fungi were examined for the ability to biotransform phenanthrene to oxidative (phase I) and conjugative (phase II) metabolites. Phenanthrene metabolites were purified by high-performance liquid chromatography (HPLC) and identified by UV/visible absorption, mass, and1H NMR spectra.Aspergillus niger ATCC 6275,Syncephalastrum racemosum UT-70, andCunninghamella elegans ATCC 9245 initially transformed [9-14C]phenanthrene to produce metabolites at the 9,10-, 1,2-, and 3,4- positions. Subsequently, sulfate conjugates of phase I metabolites were formed byA. niger, S. racemosum, andC. elegans. Minor glucuronide conjugates of 9-phenanthrol and phenanthrenetrans-9,10-dihydrodiol were formed byS. racemosum andA. niger, respectively. In addition,C. elegans produced the glucose conjugates 1-phenanthryl β-d-glucopyranoside and 2-hydroxy-1-phenanthryl β-d-glucopyranoside, a novel metabolite. [9-14C]Phenanthrene metabolites were not detected in organic extracts from biotransformation experiments with the yeasts,Candida lipolytica 37-1,Candida tropicalis ATCC 32113, andCandida maltosa R-42.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570023
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  • 2
    Electronic Resource
    Electronic Resource
    Taxol from fungal endophytes and the issue of biodiversity (1996)
    Springer
    Journal of industrial microbiology and biotechnology 17 (1996), S. 417-423 
    Details
    ISSN: 1476-5535
    Keywords: fungi ; mycology ; taxol ; Taxus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Fungi represent one of the most understudied and diverse group of organisms. Commonly, these organisms make associations with higher life forms and may proceed to biochemically mimic the host organism. An excellent example of this is the anticancer drug, taxol, which had been previously supposed to occur only in the plant genusTaxus (yew). However, taxol has been reported in a novel endophytic fungus—Taxomyces andreanae, but also has been demonstrated to occur in a number of unrelated fungal endophytes includingPestalotia, Pestalotiopsis, Fusarium, Alternaria, Pithomyces, Monochaetia and others. Thus, this report presents information on the presence of taxol among disparate fungal genera, and uses these observations as an additional argument to support efforts to study fungal endophytes and preserve their associated host plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01574772
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  • 3
    Electronic Resource
    Electronic Resource
    Recovery of fungi after storage for over a quarter of a century (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 517-519 
    Details
    ISSN: 1573-0972
    Keywords: Cryopreservation ; freeze-drying ; fungi ; oil storage ; silica gel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This paper discusses the recovery of a sample of strains originally preserved on silica gel over the period 1970–1973. Fifty-three strains were tested of which 18 recovered, demonstrating survival for more than 20 years. The recovery of 26 of the strains is directly compared with that of replicates from oil storage and freeze-drying. A summary of storage of 421 strains by the silica gel technique is given, reporting survival of 64% for a quarter of a century or more. The technique is ideal for preserving sporulating fungi of the Ascomycota and many species of mitotic fungi for laboratories of limited facilities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008959521962
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  • 4
    Electronic Resource
    Electronic Resource
    Cryogenic light microscopy and the development of cooling protocols for the cryopreservation of filamentous fungi (1997)
    Springer
    World journal of microbiology and biotechnology 14 (1997), S. 49-57 
    Details
    ISSN: 1573-0972
    Keywords: Cooling rate ; cryogenic light microscopy ; cryopreservation ; exosmosis ; fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract One hundred and ninety five strains of fungi were observed during freezing and thawing using a cryogenic light microscope. There was no obvious link between taxonomic position and their morphological response to freezing and thawing. The viability of seven of these strains was examined following freezing and thawing in the presence or absence of the cryoprotectants glycerol and dimethyl sulphoxide. Intracellular ice and hyphal shrinkage were not necessarily lethal events, but in many cases they affected the rate and quality of growth. Both cryoprotectants reduced shrinkage, shifted the cooling rate where intracellular ice formed in many cases, and improved the recovery of strains. The results presented aid the development of successful cryopreservation protocols.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008820432471
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  • 5
    Electronic Resource
    Electronic Resource
    Glucoamylase production in continuous cultures of Aspergillus niger with special emphasis on growth parameters (1997)
    Springer
    World journal of microbiology and biotechnology 14 (1997), S. 113-118 
    Details
    ISSN: 1573-0972
    Keywords: Aspergillus ; continuous culture ; glucoamylase ; growth ; fungi ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Maltose-limited continuous culture of Aspergillus niger was carried out with potassium nitrate to investigate growth and glucoamylase formation characteristics. Glucoamylase production was dependent on the specific growth rate. The maximal amount of glucoamylase (units/l and U/g dry weight) was obtained at μ=0.08h−1, and the maximum specific rate of production (units/g/dry weight per hour) was at μ=0.2h−1. The maintenance coefficients (ms and mATP) were higher than for some other fungi. Maximal growth yields on substrate, oxygen and ATP (Yxsm, YxO2m and Yxam) were very efficient (high) and the value of Yxam, which cannot exceed the theoretical maximal value, is obtained when a P/O ratio of 1:1 is assumed. This indicates that biomass formation is energetically inexpensive and most of the expended energy has to be invested in the process of glucoamylase excretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008840904246
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  • 6
    Electronic Resource
    Electronic Resource
    Technical Communication: Evaluation of cassava extract/soya bean digest medium for fungal growth (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 244-244 
    Details
    ISSN: 1573-0972
    Keywords: Cassava ; extract ; fungi ; growth ; soya bean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A medium that is cheaper than commercial media but just as good for assessing growth and viability of yeasts and fungi has been formulated using local ingredients: cassava and soya beans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00704662
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  • 7
    Electronic Resource
    Electronic Resource
    Sources of fungal linamarases (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 678-680 
    Details
    ISSN: 1573-0972
    Keywords: Cassava ; fungi ; linamarase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Forty-four strains of Aspergillus, Penicillium, Fusarium, Trichoderma and Rhizopus were grown on a liquid medium containing glucose and cassava-root extract. All of the Aspergillus and Fusarium strains, eight out of 10 Penicillium strains and three of seven Trichoderma strains showed linamarase activity. No such activity was detected in any Rhizopus strain. The crude enzyme preparation from F. oxysporum had the highest affinity for linamarin whereas that from A. nidulans was the most heat-stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00361015
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  • 8
    Electronic Resource
    Electronic Resource
    Gene pho-2 codes for the multiple active forms of Pi-repressible alkaline phosphatase in the mould Neurospora crassa (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 609-611 
    Details
    ISSN: 1573-0972
    Keywords: Alkaline phosphatase ; enzyme isoforms ; fungi ; Neurospora crassa ; pH regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The mycelial Pi-repressible alkaline phosphatase of the wild-type strain 74A of Neurospora crassa was separated into at least ten isoforms by isoelectric focusing. The components visualized by activity with sodium α-naphthyl phosphate as the substrate were predominantly acidic proteins with isoelectric points ranging from pH 4.5 to 7.6. The number of these isoforms was a function of growth pH. Strain pho-2A did not produce active Pi-repressible alkaline phosphatase (the pho-2 gene codes for its amino acid sequence), which gives an indication that these isoforms are encoded by the same structural gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018550317025
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  • 9
    Electronic Resource
    Electronic Resource
    Purification of Neurospora crassa alkaline phosphatase without DNAse activity for use in molecular biology (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 505-507 
    Details
    ISSN: 1573-0972
    Keywords: Alkaline phosphatase ; fungi ; molecular biology ; Neurospora crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Alkaline phosphatase, excreted by Neurospora crassa preg c and purified to apparent homogeneity by 7.5% PAGE, did not show DNAase activity and removed the terminal 5′-phosphate group from plasmid Bluescript M13+ linearized with EcoRI. The preg c strain may therefore replace other sources of alkaline phosphatase for use in dephosphorylating linearized plasmidial DNA in cloning experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286362
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  • 10
    Electronic Resource
    Electronic Resource
    Short communication: Purification and properties of acid phosphatase (EC 3.1.3.2) secreted by strain 74A of the mould Neurospora crassa (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 109-110 
    Details
    ISSN: 1573-0972
    Keywords: Acid phosphatase ; enzyme secretion ; fungi ; Neurospora crassa ; phosphate regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Both Pi-repressible acid phosphatases, IIb (mycelial) and IIc (extracellular), synthesized by Neurospora crassa and purified to apparent homogeneity by 7.5% PAGE, are monomers, are inhibited by 2 mm ZnCl2 and are non-specifically stimulated by salts. However, the IIc form is activated by p-nitrophenylphosphate (in a negative co-operativity effect with a K 0.5 of 2.5 mm) whereas form IIb shows Michaelis kinetics, with a K m of 0.5 mm. Thus, since both enzymatic forms may be expressed by the same gene (pho-3), it is possible that post-translational modifications lead to the excretion of an enzymatic form with altered Michaelis kinetics compared with the enzymatic form retained by the mycelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327816
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  • 11
    Electronic Resource
    Electronic Resource
    Factors determining rock phosphate solubilization by microorganisms isolated from soil (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 567-572 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; fungi ; rock phosphate ; solubilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Forty two soil isolates (31 bacteria and 11 fungi) were studied for their ability to solubilize rock phosphate and calcium phosphate in culture medium. Eight bacteria and 8 fungi possessed solubilizing ability. Pseudomonas cepacia and Penicillium purpurogenum showed the highest activity. There was a correlation between final pH value and titratable acidity (r=−0.29 to −0.87) and between titratable acidity and soluble phosphate (r=0.22 to 0.99). Correlation values were functions of insoluble phosphate and of the group of microorganisms considered. A high correlation was observed between final pH and soluble phosphate only for the rock phosphates inoculated with the highest concentration of solubilizing bacteria (r=−0.73 to −0.98).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327716
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  • 12
    Electronic Resource
    Electronic Resource
    The mechanism of cadmium removal from aqueous solution by nonmetabolizing free and immobilized live biomass of Rhizopus oligosporus (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 571-578 
    Details
    ISSN: 1573-0972
    Keywords: Biomass ; biosorption ; cadmium ; fungi ; immobilized ; Rhizopus oligosporus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A preliminary study on the removal of cadmium by nonmetabolizing live biomass of Rhizopus oligosporus from aqueous solution is presented. The equilibrium of the process was in all cases well described by the Langmuir sorption isotherm, suggesting that the process was a chemical, equilibrated and saturable mechanism which reflected the predominantly site-specific mechanism on the cell surface. A curve of Scatchard transformation plots reflected the covalent nature of Cd2+ adsorption by the cells. The maximum cadmium uptake capacities were 34.25 mg/g for immobilized cells and 17.09 mg/g for free cells. Some factorial experiments in shake flasks were performed in order to investigate the effect of different initial cadmium concentrations and biomass concentrations on the equilibrium. Experimental results showed a reverse trend of the influence of the immobilized and free biomass concentration on the cadmium specific uptake capacity. The immobilized cells had a higher specific cadmium uptake capacity with increasing biomass concentrations compared to free cells. In a bioreactor, the cadmium uptake capacity of immobilized cells (qmax = 30.1–37.5 mg/g) was similar to that observed in shake flask experiments (qmax = 34.25 mg/g) whereas with free cells the bioreactor qmax of 4.8–13.0 mg/g; was much lower than in shake flasks (qmax = 17.09 mg/g), suggesting that cadmium biosorption by immobilized cells of R. oligosporus might be further improved in bigger reactors. EDAX and transmission electron microscopic experiments on the fungal biomass indicated that the presence of Cd2+ sequestrated to the cell wall was due to bioadsorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008911601490
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  • 13
    Electronic Resource
    Electronic Resource
    Qualitative assessment of hydrolytic activities in antarctic microfungi grown at different temperatures on solid media (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 131-132 
    Details
    ISSN: 1573-0972
    Keywords: Antarctic ; fungi ; hydrolytic activities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Microfungi from Antarctica were grown at 10 °C, 21 °C, 28 °C and 37 °C on a series of plates each containing a single carbon source and designed to indicate the secretion of particular hydrolytic enzymes. Colony radius and hydrolytic activity were measured and a relative activity index (RA) established. In general, effective hydrolysis occurred at mesophilic temperatures. Some enzymes, especially of Trichoderma spp. and Penicillium spp. showed maximum activity at 10 °C, indicating adaptation to the colder temperatures of the antarctic environment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008855406319
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  • 14
    Electronic Resource
    Electronic Resource
    Microbial diversity: ex situ conservation of Indonesian microorganisms (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 359-361 
    Details
    ISSN: 1573-0972
    Keywords: Biodiversity ; conservation ; fermented foods ; fungi ; Indonesia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This paper discusses the role of microbial resource collections in the ex situ conservation of microbial diversity and, in particular, the activities of a recently completed Darwin Initiative project to facilitate collection, identification and exploitation of Indonesian microbial diversity. This helped revitalize the collection of the Lembaga Ilmu Pengetahuea Indonesia (Indonesian Institute of Sciences), LIPI, Research and Development Centre for Biology, Bogor, Indonesia. It is suggested that a focus on microorganisms beneficial to man is essential to enable ex situ collections to be a useful tool in the conservation and sustainable utilization of microbial diversity. This task is enormous and far beyond the capabilities of one collection and its achievement will necessitate a network of collections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018559729803
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  • 15
    Electronic Resource
    Electronic Resource
    Intraspecific hybridization between Coprinus cinereus and Schizophyllum commune by PEG-induced protoplast fusion and electrofusion (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 585-590 
    Details
    ISSN: 1573-0972
    Keywords: Coprinus cinereus ; electro-fusion ; fungi ; polyethylene glycol ; protoplast ; Schizophyllum commune
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two irreversible inhibitors, iodoacetamide and diethylpyrocarbonate, were used to select intraspecific fusion products of two mushroom species, Coprinus cinereus and Schizophyllum commune. Iodoacetamide was the more suitable inhibitor because it gave a low breakage frequency and low survival rate of the cells in the inactivation experiments. Fusion-induced by polyethylene glycol and electro-fusion were compared and, under optimal conditions, gave fusion frequencies of 16.7% to 50.0% and 6.9% to 8.4%, respectively. All fusion progeny were heterokaryons (dikaryons) and had clamp connections. There were no differences in the morphology and fruiting ability of the fusion progeny and those of the heterokaryons generated from mating.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286378
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  • 16
    Electronic Resource
    Electronic Resource
    Arachidonic-acid production by species of Mortierella (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 91-96 
    Details
    ISSN: 1573-0972
    Keywords: Arachidonic acid ; fatty acids ; fungi ; lipids ; Mortierella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A growth-inhibiting, aspirin-containing medium was developed to select arachidonic-acid-(ARA)-producing Mortierella species and to determine the fatty-acid content of 87 Mortierella strains. ARA was detected in 66 strains from 33 species and its production may prove useful for systematic studies on Mortierella spp. The ARA content of the 66 producing strains tested ranged from 4% to 55% of total lipids. Most of the ARA-producing strains of Mortierella (59 strains) grown on potato/glucose/agar synthesized 〈20% ARA. Four strains produced between 20% and 25% ARA and three produced 〉40%. An inverse relationship was observed between ARA and oleic-acid contents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327809
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  • 17
    Electronic Resource
    Electronic Resource
    Short communication: Decolourization of Crystal Violet by fungi (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 601-602 
    Details
    ISSN: 1573-0972
    Keywords: Crystal Violet ; dyes ; fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Crystal Violet was decolourized by Coriolus versicolor, Funalia trogii, Laetiporus sulphureus and Phanerochaete chrysosporium ME446 by 92%, 82%, 86% and 62%, respectively, over 3 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286384
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