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  • 1
    Publication Date: 2002-06-29
    Description: Efforts to influence the biology of blood vessels by gene delivery have been hampered by a lack of targeting vectors specific for endothelial cells in diseased tissues. Here we show that a cationic nanoparticle (NP) coupled to an integrin alphavbeta3-targeting ligand can deliver genes selectively to angiogenic blood vessels in tumor-bearing mice. The therapeutic efficacy of this approach was tested by generating NPs conjugated to a mutant Raf gene, ATPmu-Raf, which blocks endothelial signaling and angiogenesis in response to multiple growth factors. Systemic injection of the NP into mice resulted in apoptosis of the tumor-associated endothelium, ultimately leading to tumor cell apoptosis and sustained regression of established primary and metastatic tumors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hood, John D -- Bednarski, Mark -- Frausto, Ricardo -- Guccione, Samira -- Reisfeld, Ralph A -- Xiang, Rong -- Cheresh, David A -- CA50286/CA/NCI NIH HHS/ -- P41 RR09784/RR/NCRR NIH HHS/ -- T32 CA09696/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2002 Jun 28;296(5577):2404-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12089446" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine Triphosphate/metabolism ; Animals ; Apoptosis ; Cations ; Endothelium, Vascular/cytology/metabolism/pathology ; Gene Targeting ; Gene Transfer Techniques ; Genetic Therapy/*methods ; Genetic Vectors ; Humans ; In Situ Nick-End Labeling ; Ligands ; Lipids ; Melanoma, Experimental/blood supply/pathology/therapy ; Mice ; Mice, Inbred BALB C ; Mutation ; *Nanotechnology ; Neoplasm Metastasis ; Neoplasm Transplantation ; Neoplasms, Experimental/blood supply/pathology/*therapy ; Neovascularization, Pathologic/pathology/*therapy ; Proto-Oncogene Proteins c-raf/*genetics/metabolism ; Random Allocation ; Receptors, Vitronectin/*metabolism ; Tumor Cells, Cultured
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 2022-03-06
    Description: This work presents the data on the content of cadmium and arsenic in the viscera (visceral mass) of the veined rapa whelk Rapana venosa caught in the Azov Sea (Kerch Strait) and the Black Sea (Feodosia Gulf). In the viscera of the individuals from the Azov Sea, cadmium content was 8.6 mg/kg, and for the individuals from the Black Sea, this value was 3.5, with the allowable level 2.0 mg/kg as stipulated by TR CU 021/2011. Cadmium content in various internal organs of the investigated veined rapa whelk individuals varied. The highest content of cadmium (48 mg/kg) was recorded in the kidney of the individuals caught in the Azov Sea; cadmium content in the digestive gland also exceeded the allowable level. Arsenic content in the viscera of the individuals from the Azov Sea exceeded the allowable level by 7.8 %; this value was significantly lower for the Black Sea (0.1 mg/kg, while the established norm is 5.0 mg/kg). In several organs of the individuals from the Azov Sea (namely, the kidney, ovaries/testes, and digestive gland), arsenic content was within the normal values established by the regulations. The product derived from veined rapa whelk through enzymolysis and subsequent concentration by evaporation, was characterized by increased content of cadmium and arsenic. In terms of cadmium and arsenic content, ovaries and testes of the investigated individuals proved to be safe for consumption; their yield amounts to 10.9 % out of the entire visceral mass, and it is possible to use them as a source of valuable nutrients.
    Description: В работе приведены данные о содержании кадмия и мышьяка во внутренностях (висцеральной массе) рапаны Rapana venosa, выловленной в Азовском море (Керченский пролив) и Черном море (Феодосийский залив). Во внутренностях рапаны из Азовского моря содержание кадмия составляло 8,6 мг/кг, у рапаны из Черного моря — 3,5 мг/кг при допустимом уровне 2,0 мг/кг в соответствии с ТР ТС 021/2011. Отмечено различное содержание кадмия во внутренних органах рапаны. Почка рапаны, выловленной в Азовском море, является органом с самым высоким содержанием кадмия (48 мг/кг), несоответствие кадмия допустимому уровню установлено также в пищеварительной железе. Содержание мышьяка во внутренностях рапаны из Азовского моря на 7,8 % превышало допустимый уровень, у рапаны из Черного моря было значительно ниже — 0,1 мг/кг (норма 5,0 мг/кг). В отдельных органах рапаны из Азовского моря (почка, яичник/семенник, пищеварительная железа) содержание мышьяка отвечало установленным требованиям. Продукт, полученный в результате ферментолиза и последующего упаривания ферментолизата, характеризовался повышенным содержанием кадмия и мышьяка. Безопасными по содержанию кадмия и мышьяка являлись яичники/семенники рапаны, выход которых составлял 10,9 % от массы внутренностей. Они могут быть использованы для получения ценных нутриентов.
    Description: Published
    Description: Non Refereed
    Keywords: Rapana venosa ; Food safety ; Lipids ; Visceral mass ; Enzymolysis ; Kidneys ; Ovaries ; Cadmium ; Безопасность питания ; Жиры ; Почки ; Кадмий ; Ферментолиз ; Висцеральная масса ; ASFA_2015::P::Processing fishery products ; ASFA_2015::M::Marine molluscs ; ASFA_2015::A::Arsenic
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: pp.95-102
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  • 3
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: Coral physiological and δ15N isotopic measurements.
    Description: Experimental coral physiological and δ15N isotopic measurements in October 2012 at Reef Systems Coral Farm, Ohio. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/839920
    Description: NSF Emerging Frontiers Division (NSF EF) EF-1041124
    Keywords: Coral calcification ; Photosynthesis ; Carbon budgets ; Chlorophyll a ; Protein ; Carbohydrates ; Lipids ; Energy reserves ; Nitrogen isotopes
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 4
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Gosselin, K. M., Nelson, R. K., Spivak, A. C., Sylva, S. P., Van Mooy, B. A. S., Aeppli, C., Sharpless, C. M., O’Neil, G. W., Arrington, E. C., Reddy, C. M., & Valentine, D. L. Production of two highly abundant 2-methyl-branched fatty acids by blooms of the globally significant marine cyanobacteria Trichodesmium erythraeum. ACS Omega, 6(35), (2021): 22803–22810, https://doi.org/10.1021/acsomega.1c03196.
    Description: The bloom-forming cyanobacteria Trichodesmium contribute up to 30% to the total fixed nitrogen in the global oceans and thereby drive substantial productivity. On an expedition in the Gulf of Mexico, we observed and sampled surface slicks, some of which included dense blooms of Trichodesmium erythraeum. These bloom samples contained abundant and atypical free fatty acids, identified here as 2-methyldecanoic acid and 2-methyldodecanoic acid. The high abundance and unusual branching pattern of these compounds suggest that they may play a specific role in this globally important organism.
    Description: This work was funded with grants from the National Science Foundation grants OCE-1333148, OCE-1333162, and OCE-1756254 and the Woods Hole Oceanographic Institution (IR&D). GCxGC analysis made possible by WHOI’s Investment in Science Fund.
    Keywords: Lipids ; Alkyls ; Bacteria ; Genetics ; Chromatography
    Repository Name: Woods Hole Open Access Server
    Type: Article
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