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  • Other Sources  (60)
  • Elsevier  (50)
  • John Wiley & Sons, Ltd  (10)
  • Oxford Univ. Press
  • 2020-2022  (60)
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  • 1
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    Elsevier
    In:  In: Fundamentals of Tropical Freshwater Wetlands. , ed. by Dalu, T. and Wasserman, R. J. Elsevier, Amsterdam, The Netherlands, pp. 517-547. ISBN 978-0-12-822362-8
    Publication Date: 2021-11-23
    Type: Book chapter , NonPeerReviewed
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  • 2
    Publication Date: 2021-07-20
    Description: A shell of subsiding air is generally known to develop around cumulus clouds and shield them from their environment. We seek here to improve our understanding of such shells by (a) revealing the detailed vertical and horizontal structure of shells surrounding both shallow and deeper clouds, and (b) identifying the mechanisms responsible for in‐shell subsidence generation and maintenance. To that end, a high‐resolution Cloud Resolving Model simulation of the shallow‐to‐deep convection transition over a tropical land surface is analysed with an emphasis on the cloud's near environment. Shells surrounding shallow and deep clouds are found to possess surprisingly similar characteristics. However important differences are observed near cloud top where the deepest clouds are associated with stronger subsidence and broader shells. In the convective outflow region, stronger in‐shell subsidence coincides with strong buoyancy reversal, but also with strong pressure gradients naturally generated by cloud‐top vortex dynamics. A more delicate balance between various processes takes place below, and in‐shell subsidence is only barely sustained as buoyancy reversal is largely compensated by pressure gradients. Finally, while evaporation is clearly the main source of buoyancy reversal everywhere around cloud edges, it is also shown that the downward transport of warmer air from aloft through the subsiding shells may compensate for evaporative cooling to slowly bring in‐shell buoyancy to a near‐neutral state. Overall, while it cannot be denied that evaporative cooling and buoyancy reversal play important roles in generating and sustaining in‐shell subsidence, the present results also emphasise that mechanical forcing at cloud top and downward transport within the shells should not be overlooked.
    Description: Narrow “shells” of subsiding air generally form around cumulus clouds, but the mechanisms responsible for their formation and maintenance are still debated. In this study, the dynamics of these shells is investigated using a high‐resolution simulation of idealized tropical convection. It is shown that in‐shell subsiding motions are generally driven by buoyancy reversal. However, mechanical forcing contributes at least equally at the top of all convective clouds. In addition, it is found that buoyancy reversal due to evaporative cooling may be offset by the downward transport of warmer air from aloft through the subsiding shells.
    Keywords: 551.5 ; cloud dynamics ; near‐cloud environment ; subsiding shells ; tropical convection
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  • 3
    Publication Date: 2021-07-20
    Description: Motivated by the question of whether and how wave–wave interactions should be implemented into atmospheric gravity‐wave parametrizations, the modulation of triadic gravity‐wave interactions by a slowly varying and vertically sheared mean flow is considered for a non‐rotating Boussinesq fluid with constant stratification. An analysis using a multiple‐scale WKBJ (Wentzel–Kramers–Brillouin–Jeffreys) expansion identifies two distinct scaling regimes, a linear off‐resonance regime, and a nonlinear near‐resonance regime. Simplifying the near‐resonance interaction equations allows for the construction of a parametrization for the triadic energy exchange which has been implemented into a one‐dimensional WKBJ ray‐tracing code. Theory and numerical implementation are validated for test cases where two wave trains generate a third wave train while spectrally passing through resonance. In various settings, of interacting vertical wavenumbers, mean‐flow shear, and initial wave amplitudes, the WKBJ simulations are generally in good agreement with wave‐resolving simulations. Both stronger mean‐flow shear and smaller wave amplitudes suppress the energy exchange among a resonantly interacting triad. Experiments with mean‐flow shear as strong as in the vicinity of atmospheric jets suggest that internal gravity‐wave dynamics are dominated in such regions by wave modulation. However, triadic gravity‐wave interactions are likely to be relevant in weakly sheared regions of the atmosphere.
    Description: This study explores wave–wave interactions of modulated internal gravity waves (GWs) in varying background flows using WKBJ techniques. The resulting ray‐tracing model (b) is compared to wave‐resolving LES (a). As a key result, we find that wave modulation partially suppresses the energy exchange in triadic GW interactions, and thus triadic GW interactions are likely to be relevant in weakly sheared regions of the atmosphere.
    Description: German Research Foundation (DFG) US National Science Foundation
    Keywords: 551.5 ; internal gravity waves ; parametrization ; ray‐tracing ; triadic wave–wave interaction ; wave modulation
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  • 4
    Publication Date: 2021-07-20
    Description: One important attribute of meteorological forecasts is their representation of spatial structures. While several existing verification methods explicitly measure a structure error, they mostly produce a single value with no simple interpretation. Extending a recently developed wavelet‐based verification method, this study separately evaluates the predicted spatial scale, orientation and degree of anisotropy. The scale component has been rigorously tested in previous work and is known to assess the quality of a forecast similar to other, established methods. However, directional aspects of spatial structure are less frequently considered in the verification literature. Since important weather phenomena related to fronts, coastlines and orography have distinctly anisotropic signatures, their representation in meteorological models is clearly of interest. The ability of the new wavelet approach to accurately evaluate directional properties is demonstrated using idealized and realistic test cases from the MesoVICT project. A comparison of precipitation forecasts from several forecasting systems reveals that errors in scale and direction can occur independently and should be treated as separate aspects of forecast quality. In a final step, we use the inverse wavelet transform to define a simple post‐processing algorithm that corrects the structural errors. The procedure improves visual similarity with the observations, as well as the objective scores.
    Description: Forecasts of precipitation fields are difficult to evaluate due to their complex, intermittent spatial structure. The SAD forecast verification method uses wavelets to compare the scale (colours in the top row), anisotropy (bottom, arrow length) and preferred direction (bottom, arrow angles) of simulated and observed fields. The new approach is successfully tested using data from the MesoVICT community project.
    Description: German Research Foundation (DFG)
    Keywords: 551.5 ; MesoVICT ; precipitation forecasts ; structure error ; verification ; wavelets
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  • 5
    Publication Date: 2021-07-20
    Description: Cold pools originate from evaporation in precipitating downdraughts and spread as density currents at the surface. Vertical motion at the leading edge of the cold pool is an important trigger for new convective cells in organised convective storms. However, these motions are poorly resolved at the grid lengths of a kilometre or more used in convection‐permitting models. Consequently, the simulated gust fronts do not trigger enough new convection, leading to precipitation deficits and a lack of convective organization. To address these deficits, we introduce a cold pool perturbation (CPP) scheme that strengthens vertical velocity at the simulated cold pool gust fronts. This is achieved by relaxing the vertical velocity in the gust front region towards a target value derived from similarity theory. Applying the CPP scheme for simulations of a highly convective 10‐day period, we find increased precipitation amplitudes during the afternoon. There is also evidence for improvements in the location of precipitation and for stronger organization of convection, although substantial errors remain. The cold pools themselves become more frequent, larger and more intense. An additional potentially beneficial influence was found for convective initiation at sea breeze fronts.
    Description: We develop a cold pool perturbation scheme which strengthens the too weak vertical velocities at cold pool gust fronts in convection‐permitting models. In so doing, cold‐pool‐driven convective initiation is enhanced and simulated precipitation improved.
    Keywords: 551.5 ; cold pools ; convective organization ; deep convection ; density currents ; gust fronts
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  • 6
    Publication Date: 2021-07-20
    Description: The transport of trace gases by the atmospheric circulation plays an important role in the climate system and its response to external forcing. Transport presents a challenge for Atmospheric General Circulation Models (AGCMs), as errors in both the resolved circulation and the numerical representation of transport processes can bias their abundance. In this study, two tests are proposed to assess transport by the dynamical core of an AGCM. To separate transport from chemistry, the tests focus on the age‐of‐air, an estimate of the mean transport time by the circulation. The tests assess the coupled stratosphere–troposphere system, focusing on transport by the overturning circulation and isentropic mixing in the stratosphere, or Brewer–Dobson Circulation, where transport time‐scales on the order of months to years provide a challenging test of model numerics. Four dynamical cores employing different numerical schemes (finite‐volume, pseudo‐spectral, and spectral‐element) and discretizations (cubed sphere versus latitude–longitude) are compared across a range of resolutions. The subtle momentum balance of the tropical stratosphere is sensitive to model numerics, and the first intercomparison reveals stark differences in tropical stratospheric winds, particularly at high vertical resolution: some cores develop westerly jets and others easterly jets. This leads to substantial spread in transport, biasing the age‐of‐air by up to 25% relative to its climatological mean, making it difficult to assess the impact of the numerical representation of transport processes. This uncertainty is removed by constraining the tropical winds in the second intercomparison test, in a manner akin to specifying the Quasi‐Biennial Oscillation in an AGCM. The dynamical cores exhibit qualitative agreement on the structure of atmospheric transport in the second test, with evidence of convergence as the horizontal and vertical resolution is increased in a given model. Significant quantitative differences remain, however, particularly between models employing spectral versus finite‐volume numerics, even in state‐of‐the‐art cores.
    Description: The climatological and zonal mean zonal wind ū (m·s−1), as simulated by two different dynamical cores, (left) pseudospectral (GFDL‐PS) and (right) finite‐volume (CAM‐FV), with (top) 40 vertical levels and (bottom) 80 vertical levels. With higher vertical resolution, the pseudospectral core develops westerlies in the tropical stratosphere between 20 and 80 hPa, while the finite‐volume core consistently simulates easterlies at both vertical resolutions. Both cores have comparable horizontal resolution. The contour interval is 10 m·s−1.
    Description: US National Science Foundation
    Keywords: 551.5 ; age of air ; Brewer–Dobson circulation ; dynamical cores ; stratospheric dynamics ; tracer transport.
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  • 7
    Publication Date: 2021-07-20
    Description: A non‐eddy‐resolving microscale model is applied to simulate convection over three different leads (elongated channels in sea ice), which were observed by aircraft over the Arctic Marginal Ice Zone in 2013. The study aims to evaluate the quality of a local and a non‐local turbulence parametrization. The latter represents a lead‐width‐dependent approach for the turbulent fluxes designed for idealised conditions of a lead‐perpendicular, near‐neutral inflow in an atmospheric boundary layer (ABL) capped by a strong inversion at around 250 to 350 m height. The observed cases considered here are also characterised by an almost lead‐perpendicular flow but, in comparison to the idealised conditions, our analysis covers effects in stable inflow conditions and a much shallower ABL. The model simulations are initialised with observed surface parameters and upwind profiles, and the results are compared with measurements obtained above and downwind of the leads. The basic observed features related to the lead‐generated convection can be reproduced with both closures, but the observed plume inclination and vertical entrainment near the inversion layer by the penetrating plume are underestimated. The advantage of the non‐local closure becomes obvious by the more realistic representation of regions with observed vertical entrainment or where the observations hint at counter‐gradient transport. It is shown by comparison with the observations that results obtained with the non‐local closure can be further improved by including the determination of a fetch‐dependent inversion height and by specifying a parameter determining the plume inclination as a function of the upwind ABL stratification. Both effects improve the representation of fluxes, boundary‐layer warming, and vertical entrainment. The model is also able to reproduce the observed vanishing of a weak low‐level jet over the lead, but its downwind regeneration and related momentum transport are not always well captured, irrespective of the closure used.
    Description: In typical springtime conditions of a cold atmospheric flow over the warm surfaces of leads, which are open‐water channels in sea ice, strong convective plumes are generated which have a large impact on atmospheric boundary‐layer characteristics. Here, a small‐scale model is applied to simulate such situations and model results obtained with different turbulence parametrizations are evaluated using airborne measurements. Based on the observations, a non‐local parametrization developed for the small‐scale modelling of the inhomogeneous convection over leads is further improved.
    Description: Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) via the Transregional Collaborative Research Center ArctiC Amplification (AC)3 (project number 268020496 TRR 172) and via the priority program SPP 1158 (grant LU 818/5‐1)
    Keywords: 551.5 ; aircraft observations ; atmospheric boundary layer ; convection over leads ; counter‐gradient transport ; entrainment ; microscale model ; sea ice ; turbulence parametrization
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  • 8
    Publication Date: 2021-07-20
    Description: We present an Arctic ocean–sea ice reanalysis covering the period 2007–2016 based on the adjoint approach of the Estimating the Circulation and Climate of the Ocean (ECCO) consortium. The spatiotemporal variation of Arctic sea surface temperature (SST), sea ice concentration (SIC), and sea ice thickness (SIT) is substantially improved after the assimilation of ocean and sea ice observations. By assimilating additional World Ocean Atlas 2018 (WOA18) hydrographic data, the freshwater content of the Canadian Basin becomes closer to the observations and translates into changes of the ocean circulation and of transports through the Fram and Davis straits. This new reanalysis compares well with previous filter‐based (TOPAZ4) and nudging‐based (PIOMAS) reanalyses regarding SIC and SST. Benefiting from using the adjoint of the sea ice model, our reanalysis is superior to the ECCOv4r4 product considering sea ice parameters. However, the mean state and variability of the freshwater content and the transport properties of our reanalysis remain different from TOPAZ4 and ECCOv4r4, likely because of a lack of hydrographic observations.
    Description: Arctic sea ice has declined rapidly and reached a record minimum in September, 2012. Arctic ocean–sea ice reanalyses are invaluable sources for understanding the Arctic sea ice changes. We produce an Arctic ocean–sea ice reanalysis of the years 2007–2016 using the adjoint method. The reanalysis is dynamically consistent without introducing unphysical mass and energy discontinuities as in filter‐based data assimilation methods.
    Keywords: 551 ; adjoint method ; data assimilation ; ocean–sea ice reanalysis
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  • 9
    Publication Date: 2021-07-20
    Description: A model for studying Transient Inertia–Gravity And Rossby wave dynamics (TIGAR) is introduced. The presented horizontal component of the model solves the nonlinear rotating shallow‐water equations on the sphere using Hough harmonics. Spectral modelling using Hough harmonics as basis functions describes atmospheric dynamics in terms of physically identifiable structures: Rossby and inertia–gravity eigensolutions of linearized primitive equations. This offers an attractive framework for detangling gravity wave dynamics in high‐resolution simulations. Accurate computations are achieved through the use of higher order integrating factor and exponential time‐differencing methods, leading to a major increase in computational efficiency and stability. A comparison with classical time‐stepping schemes shows accuracy improvements of several orders of magnitude at no additional computational cost. In particular, stability gains are achieved through enhanced accuracy and efficiency in the computation of gravity waves, rather than through their damping. In the new framework, reduced models using Rossby and gravity waves aimed at studying dynamical aspects of data assimilation or wave interactions are easily implemented.
    Description: We present new global forecast model for Transient Inertia–Gravity And Rossby wave dynamics (TIGAR). By decomposing the flow into Rossby and gravity wave components and employing exponential time‐differencing schemes, TIGAR achieves remarkable gains in accuracy, efficiency, and stability. The figure compares forecast errors in Rossby and gravity waves computed by TIGAR with different time‐stepping algorithms in a barotropic instability test at T170 resolution.
    Description: UHH Ideen‐ und Risikofund
    Keywords: 551.52 ; exponential time‐stepping schemes ; gravity waves ; Hough harmonics ; nonlinear wave dynamics ; numerical model accuracy ; Rossby waves
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  • 10
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    John Wiley & Sons, Ltd | Chichester, UK
    Publication Date: 2021-07-20
    Description: Four idealized, high‐resolution (500 m horizontal grid spacing), numerical simulations are used to investigate the evolution of convective structures during tropical cyclogenesis. The simulations all begin with a weak initial axisymmetric cloud‐free vortex in a quiescent environment, but differ in the moisture level of the initial sounding and whether or not ice microphysical processes are considered. Irrespective of experimental setup, there is only a short period where shallow or congestus clouds dominate. The shallow cloud phase is slightly extended with the drier initial environmental sounding. The composite structure of the convective elements sampled changes markedly throughout the genesis period. For much of the genesis phase, vertical profiles of the mean convective cell show significant amounts of anticyclonic vorticity produced in cells in the inner core. Towards the end of the genesis phase, there is a large increase in the production of cyclonic vertical vorticity in inner‐core convection, and cyclonic vorticity becomes dominant at low‐mid levels. The evolution from roughly equal strength vertical profiles of cyclonic/anticyclonic vorticity at low‐mid levels to profiles where cyclonic vorticity dominates occurs at relatively low system wind speeds (Vmax less than 10 m·s−1). This finding indicates a change in the structure of vortical convection prior to rapid intensification. In outer‐core convection, there are roughly equal strength vertical vorticity dipoles produced throughout the genesis period.
    Description: Four idealized, high‐resolution (500 m horizontal grid spacing), numerical simulations are used to investigate the evolution of convective structures during tropical cyclogenesis. The composite structure of the average convective element sampled changes markedly throughout the genesis period, and towards the end of the genesis phase there is a large increase in the production of cyclonic vertical vorticity in inner‐core convection, which becomes dominant at low‐mid levels. Irrespective of microphysical scheme and environmental sounding used, there is a short period where cumulus congestus clouds dominate, that is, when the mean cloud top is between 5 and 9 km height, a finding which is at odds with a prior theory claiming that tropical cyclogenesis can be viewed as a two‐stage process in which congestus clouds have a large dynamical and thermodynamical contribution at early stages.
    Description: German Research Council (Deutsche Forschungsgemeinschaft) http://dx.doi.org/10.13039/501100001659
    Keywords: 551.5 ; convection ; cyclogenesis ; hurricane ; tropical cyclone
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  • 11
    Publication Date: 2021-06-20
    Description: Motivated by prior research examining the myth of inertial stability as a radial restoring force in the tropical cyclone boundary layer, we explore factors determining the vertical velocity at the top of the linear vortex boundary layer. Possible applications of these findings to mature tropical cyclone vortices are discussed briefly.
    Keywords: 551.51 ; boundary layer ; generalized Ekman balance ; inertial stability ; tropical cyclones
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  • 12
    Publication Date: 2021-02-08
    Description: Eco-friendly, cost efficient and effective extraction methods have become significant for the industries applying zero waste principles. The two main objectives of this study were; to examine fucoxanthin extraction from wet Phaeodactylum tricornutum using subcritical fluid extraction and to characterize the residual biomass in order to determine the potential application areas. The highest fucoxanthin yield of 0.69 ± 0.05 mg/g wet cell weight was achieved using methanol with solvent-to-solid ratio of 200:1 at 120 rpm, 20 MPa pressure and at 35 °C for 60 min by subcritical extraction. Microscopy images showed that most of the cells were disrupted and intracellular components were effectively released. Based on the results of energy dispersive spectroscopy, biomass contained a mixture of organic molecules including mainly carbon (57–72%), oxygen (26–41%), magnesium (0.6–1.4%) and silica (0.4–1%) (wt%). These results make the residual biomass a potential candidate for various areas such as bioenergy, material sciences and sensor technologies.
    Type: Article , PeerReviewed
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  • 13
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    Elsevier
    In:  Progress in Oceanography, 189 . Art.-Nr.: 102452.
    Publication Date: 2021-02-08
    Description: Subsurface-intensified anticyclones are ubiquitous in the ocean, yet their impact on the large-scale transport of heat, salt and chemical tracers is poorly understood. These submesoscale coherent vortices (SCVs) can trap and advect waters thousands of kilometers away from the formation region, providing a transport pathway that is unresolved by low-resolution Earth System Models. However, knowledge of the importance of these eddies for the large scale circulation is hindered by the lack of systematic observations. Here, we take advantage of the global network of Argo floats to identify occurrences of these eddies, which appear as weakly stratified anomalous water masses with Gaussian-shaped vertical structures. We develop a general algorithm to detect subsurface eddies that have propagated away from their source region, and apply it to the database of Argo float profiles, resulting in roughly 4000 detections from more than 20 years of observations. We further group detections into regional populations to identify hot-spots of generation and mechanisms of formation. Analysis of regional SCV statistics reveals important sites of SCV generation in Eastern Boundary Upwelling Systems, marginal sea overflows, and mode water formation regions along major open-ocean fronts. Because of the heat and salt anomaly contained within their cores, SCV could leave a significant imprint on the hydrographic properties of water masses in regions of high SCV density.
    Type: Article , PeerReviewed
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  • 14
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    Elsevier
    In:  In: Encyclopedia of Geology. , ed. by Elias, S. and Alderton, D. Elsevier, London, pp. 52-59, 8 pp. 2nd edition ISBN 978-0-08-102909-1
    Publication Date: 2021-01-26
    Description: The vast majority of active volcanism that is located at plate boundaries can be easily explained by plate tectonic processes. Intraplate volcanism, which incorporates some of the smallest and largest volcanic events on Earth, cannot be successfully explained by a single process or model. The most volumetrically significant intraplate volcanic events are associated with the arrival of the head of a thermo-chemical anomaly rising from the deep mantle and impacting the base of the lithosphere. This event generates massive and short-lived magmatic activity over a wide area (up to 2000 km across), forming a large igneous province. A long-lived hotspot track can form over the mantle plume tail and is best illustrated by the formation of age progressive volcanic chains, such as the famous Hawaiian-Emperor chain. Millions of smaller solitary volcanic edifices, non-age progressive volcanic chains and provinces, on the other hand, have other potential mechanisms of origin. Potential models comprise decompression melting due to lithospheric extension, destabilization of fusible lithologies in the lithospheric mantle, small scale sub-lithospheric convection, or lithospheric delamination.
    Type: Book chapter , NonPeerReviewed
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  • 15
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    Elsevier
    In:  In: Sustainable Seaweed Technologies. , ed. by Torres, M. D., Kraan, S. and Dominguez, H. Elsevier, Amsterdam, Netherlands, pp. 613-639, 27 pp. ISBN 978-0-12-817943-7
    Publication Date: 2021-01-11
    Description: The concept of biosorption results from the “passive” (nonmetabolic) interaction of a chemical species with a particle of a biological material. This interaction can be practically exploited, for example, for removal of toxic substances of wastewaters or for the enrichment of a fertilizer with micronutrients. In this work, equilibrium and dynamic data obtained with “low-cost” biomaterials of algal waste or invasive seaweed species have been reviewed and critically analyzed in the context of a circular economy. The concept of a biosorption unit, oriented to the valorization of any solid residual material of algal biomass, can be considered as a real possibility in a biorefinery process; but to accomplish this goal it is necessary that the emphasis on research must definitely move from laboratory scale toward pilot plant assays.
    Type: Book chapter , NonPeerReviewed
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  • 16
    Publication Date: 2021-01-08
    Description: Gas hydrates dissociation could induce or trigger submarine landslides, especially in upper continental slopes where hydrates are vulnerable to natural and artificial perturbations. This work investigates destabilization mechanisms of an upper continental slope undergoing hydrate dissociation and identifies spatiotemporal failure modes influenced by characteristics of the overburden above the hydrate-bearing layer (i.e. the hydrate reservoir). A Thermo-Hydro-Chemical coupled numerical model of transient pore pressure induced by hydrate dissociation is coupled with the limit equilibrium slope analysis method to study the spatiotemporal evolution of the potential sliding plane and to calculate the corresponding factor of safety. The results suggest that overpressure generated by the liberated fluid from hydrate dissociation is the primary reason for instability in a gentle marine slope. The study identifies three sliding modes, namely co-melting non-interface sliding, co-melting interface sliding, and post-melting non-interface sliding, depending on the overburden's characteristics, including overburden thickness, permeability, and cohesion. Co-melting non-interface sliding takes place during hydrate dissociation if the hydrate reservoir underlies a thin, pervious and low-cohesion overburden cover. For less permeable and more cohesive overburdens, the potential sliding plane is deeper and co-melting interface sliding could be triggered due to overpressure developed at the reservoir-overburden interface. If the hydrate reservoir is covered by a thick, low-permeability and slightly cohesive overburden, post-melting non-interface sliding could occur after the hydrates are completely dissociated. This failure is delayed, because the gas/water trapped at the interface during hydrate dissociation is insufficient to trigger instability due to very high overburden stresses. However, as the gas migrates upwards over time and encounters a weak zone in the overburden deposits, failure could happen within the overburden deposits even after hydrate dissociation stops. The findings help to improve our fundamental understanding about the destabilization mechanism and failure modes of the continental slopes undergoing hydrate dissociation, and to delineate the vulnerable configurations of the slopes.
    Type: Article , PeerReviewed
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  • 17
    Publication Date: 2021-01-08
    Description: n our daily lives, we consume foods that have been transported, stored, prepared, cooked, or otherwise processed by ourselves or others. Food storage and preparation have drastic effects on the chemical composition of foods. Untargeted mass spectrometry analysis of food samples has the potential to increase our chemical understanding of these processes by detecting a broad spectrum of chemicals. We performed a time-based analysis of the chemical changes in foods during common preparations, such as fermentation, brewing, and ripening, using untargeted mass spectrometry and molecular networking. The data analysis workflow presented implements an approach to study changes in food chemistry that can reveal global alterations in chemical profiles, identify changes in abundance, as well as identify specific chemicals and their transformation products. The data generated in this study are publicly available, enabling the replication and re-analysis of these data in isolation, and serve as a baseline dataset for future investigations.
    Type: Article , PeerReviewed
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  • 18
    Publication Date: 2021-01-08
    Description: A comparison of gouge and hammer coring techniques in intertidal wetland soils highlights a significant effect of soil compaction of up to 28% associated with the widely applied hammer coring method employed in Blue Carbon research. Hammer coring reduces the thickness of the soil profile and increases the dry bulk density, which results in an overestimation of the soil OC stock of up to 22%. In saltmarshes with multiple different soil units, we show that hammer coring is unsuitable for the calculation of OC stocks and should be avoided in favour of Russian or gouge cores. Compaction changes both soil dry bulk density and porosity and we show that resultant radiometric chronologies are compromised, almost doubling mass accumulation rates. While we show that the OC (%) content of these sediments is largely unchanged by coring method, the implication for OC burial rates are profound because of the significant effect of hammer coring on the calculation of soil mass accumlation rates.
    Type: Article , PeerReviewed
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  • 19
    Publication Date: 2021-01-08
    Description: Seagrasses provide multiple ‘ecosystem services' in coastal waters, including carbon sequestration. However, this ‘Blue Carbon’ potential has been only evaluated for certain species from some areas of the world. In this study, we provide initial estimates on the magnitude and local variability of carbon sequestration, as organic carbon stocks, for seagrass meadows of Cymodocea nodosa (Ucria) Ascherson in the oceanic island of Gran Canaria (Canary Islands, Spain, central-eastern Atlantic). Six seagrass meadows were selected; at each meadow, cores inserted up to 30 cm in the seabed were collected in the ‘interior’, ‘edge’ and ‘unvegetated’ bottoms immediately adjacent to seagrass patches. We estimated organic carbon (Corg) pools by means of the Loss of Ignition (LOI) procedure. Overall, larger Corg pools were observed in the ‘interior’ and 'edges' of meadow patches than in adjacent ‘unvegetated’ bottoms. At the meadow-level, Corg pools were not predicted neither by the meadow area, nor by the mean shoot density, or sediment grain fractions. Overall, the total estimated stock was 86.20 ± 19.06 Mg C ha−1. By considering the total potential extension of seagrass meadows across the entire island perimeter, we estimated a total stock of 60.34 Gg of C, for a mean estimated financial value of 919,432.249 € (1313.47 € ha−1), which ranges between 351,631.35 € (502.33 € ha−1) and 1,498,954.45 € (2141.36 € ha−1), according to varying market prices in the last 5 years. This work highlights, therefore, the importance of meadows underpinned by C. nodosa not only at an ecological, but also at an economic level, in particular from the perspective of regional climate change adaptation strategies.
    Type: Article , PeerReviewed
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  • 20
    Publication Date: 2021-01-08
    Description: Ciona intestinalis is within the group of taxa that are spreading globally and is one of the most thriving invasive marine species, known to depress both species richness and abundance at a local scale in distinct geographic areas. It is a dominant biofouling agent, associated with the decreasing economic incomes from aquaculture operations around the world. Ciona intestinalis was first observed in 2007 at Straumsvík in Southwest Iceland. The present study was designed to provide general information on its current distribution across Icelandic harbours. The species was found only on the SW coast of Iceland, in dense aggregations reaching up to 876 ind/m2 in all harbours from Grindavík to Akranes. The current work provides information that can be used to track the dispersal of C. intestinalis populations along the Icelandic coast and for the development of effective management decisions.
    Type: Article , PeerReviewed
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  • 21
    Publication Date: 2021-01-08
    Description: Interest in microalgae-derived products is growing, mostly due to their unique characteristics and range of industrial applications. To obtain different products, one must employ specific pretreatments that retain the properties of the biologically active compounds extracted from microalgae biomass; thus, new extraction techniques require frequent upgrades. Due to increased interest in economically viable and ecologically friendly processes, new extraction methods that can be incorporated into microalgae biorefinery systems have become the main focus of research. Therefore, this review aims to address the potential applications, future prospects, and economic scenario of the new physicochemical treatments used in the extraction of bioactive microalgae compounds.
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  • 22
    Publication Date: 2021-01-08
    Description: Long-chain polyunsaturated fatty acids (LC-PUFAs) especially ω-3 fatty acids provide significant health benefits for human beings. However, ω-3 LC-PUFAs cannot be synthesized de novo in mammals. Traditionally, ω-3 LC-PUFAs are extracted from marine fish, and their production depends on sea fishing, which has not met ever-increasing global demand. To address the challenges, innovative cellular engineering strategies need to be developed. In nature, many fungi and microalgae are rich in ω-3 LC-PUFAs, representing promising sources of ω-3 LC-PUFAs. The latest progress in developing new cellular engineering strategies toward sustainable ω-3 LC-PUFAs production using fungi and microalga has demonstrated that they can to some extent address the supply shortage. In this review, we critically summarize the recent progress in enhancing the productivity in various ω-3 LC-PUFAs-producing organisms, as well as the latest efforts of biosynthesizing PUFAs in heterogenous biosystems. In addition, we also provide future perspectives in developing genetic toolkits for LC-PUFAs producing microbes so that cut-edging biotechnology such as gene stacking and genome editing can be further applied to increase the productivity of ω-3 LC-PUFAs.
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  • 23
    Publication Date: 2021-01-08
    Description: Factors affecting carotenoid extraction with edible oils were examined using edible brown seaweed, Sargassum horneri, as main sample. The results indicate that drying was essential to extract fucoxanthin (Fx) from S. horneri and physical (boiling) and chemical (acid/alkali) pretreatment of the wet sample increased the extraction rate of Fx. Additionally, more Fx was found from the dried S. horneri powder with a smaller particle size. The extraction rate of Fx is affected by the extraction temperature and time, showing that the effective extraction would be obtained at 50 °C within 12 hr extraction. Among the oils used, short-chain (C4 and C6) triacylglycerol (TAG) (SCT) and medium-chain (C8) TAG (MCT) could extract more Fx from S. horneri and more β-carotene and lutein from spinach and olive leaves. The relatively lower viscosity of SCT and MCT would be the most likely reason for the higher extraction rates of both TAGs.
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  • 24
    Publication Date: 2021-01-08
    Description: Sargassum fusiforme polysaccharides, acidic water-soluble polysaccharides extract from Sargassum fusiforme, are mainly composed of alginic acid, fucoidan and laminaran. Alginic acid is carboxyl-containing polysaccharide formed by joining β-D-mannuronic acid and α-L-guluronic acid through β-(1→4)/α-(1→4) glycosidic bond. Fucoidan, a natural water-soluble sulfated heteropolysaccharide with fucose and sulfuric acid groups as the core structure, is mainly linked by L-fucose through α-(1→3) glycosidic bond and has the strongest biological activity. Laminaran is mainly composed of β-D-glucose through β-(1→3) glycosidic bond linkage. Sargassum fusiforme polysaccharides have a variety of pharmacological activities, including antioxidant, anti-tumor, promoting immunity, anti-aging, prompting bone growth, lowering blood glucose, anti-coagulation, anti-virus, anti-bacteria, anti-fatigue, promoting growth and development, and skin protection. These activities are closely related to the functions of fucoidan in Sargassum fusiforme polysaccharides, which fucoidan is able to strengthen immune system and antioxidation in human body. In this review, the composition, the isolation and purification, and the biological activities of Sargassum fusiforme polysaccharides are discussed and can bereference for further study.
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  • 25
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    Elsevier
    In:  Computers and Geotechnics, 124 (Article number 103596).
    Publication Date: 2021-01-08
    Description: Numerical simulation is important for practical and efficient assessment of hydrate reservoir formation stability and gas production potential. The history-matching simulation of hydrate gas production tests is complex due to coupled THM (thermo-hydro-mechanical) phenomena. The well log data from the Eastern Nankai Trough methane gas production site suggest vertically heterogeneous field properties. In the previous numerical simulation research of this site, the heterogeneous geological data were homogenized by adopting the standard mean-field theory, which can potentially lead to inaccurate simulation results due to the mesh size effect. By introducing new upscaling techniques for the permeability profiles and mechanical responses, a revised homogenization approach is proposed to improve the coupled THM simulation accuracy. In this study, seven gas production simulations of a hypothetical reservoir, six simulations of the Eastern Nankai Trough gas production test, and four simulations of the randomly generated site formation production test with different mesh sizes and different homogenization approaches were carried out to demonstrate that the proposed upscaling techniques can improve the accuracy of the simulation results with a coarse mesh model. This work, in turn, provides researchers and field engineers a much quicker way to assess the complex geomechanical behaviors of hydrate gas production site.
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  • 26
    Publication Date: 2021-01-08
    Description: Nitrous oxide is an important greenhouse gas and there is a need for sensitive techniques to study its distribution in the environment at concentrations near equilibrium with the atmosphere (9.6 nM in water at 20 °C). Here we present an electrochemical sensor that can quantify N2O in the nanomolar range. The sensor principle relies on a front guard cathode placed in front of the measuring cathode. This cathode is used to periodically block the flux of N2O towards the measuring cathode, thereby creating an amplitude in the signal. This signal amplitude is unaffected by drift in the baseline current and can be read at very high resolution, resulting in a sensitivity of 2 nM N2O for newly constructed sensors. Interference from oxygen is prevented by placing the front guard cathode in oxygen-consuming electrolyte. The sensor was field tested by measuring an N2O profile to a depth of 120 m in the oxygen minimum zone of the Eastern Tropical North Pacific Ocean (ETNP) off the coast of Mexico.
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  • 27
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    Elsevier
    In:  Progress in Oceanography, 186 . p. 102346.
    Publication Date: 2021-01-08
    Description: The ability of consumers to convert ingested carbon into growth is critical for secondary production and trophic transfer. We conducted laboratory experiments to investigate the effect of different prey and concentration on the ingestion rate (IR), egg production rate (EPR) and egg production efficiency (EPE) of the ubiquitous copepod, Acartia tonsa. Experiments were run at several prey concentrations, ranging from 11 to 1132 μgC L–1, of the diatom Thalassiosira weissflogii, the autotrophic dinoflagellate Prorocentrum minimum, the heterotrophic dinoflagellate Oxyrrhis sp., the flagellate Dunaliella tertiolecta, and the bacterivorous scuticociliate Uronema sp. IR increased curvilinearly with concentration for all diets. EPR also increased curvilinearly with increasing food concentration similar to IR, with the exception of the flagellate diet, for which EPR decreased linearly with food concentration. EPR ranked as T. weissflogii 〉 P. minimum 〉 Oxyrrhis sp. = Uronema sp. 〉 D. tertiolecta. IR and EPR were linearly related, except for flagellate diet. The slope of the carbon-based relationship between IR and EPR, the egg production efficiency (EPE), was highest for the diatom (77.5%) and lowest for the scuticociliate (4.2%). Egg production was not correlated to ingestion of the flagellate offered to A. tonsa. We conclude that of the five prey species, the diatom T. weissflogii is the best prey to promote A. tonsa reproduction, to optimize trophic transfer efficiency, and to increase mass cultivation of this species.
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  • 28
    Publication Date: 2021-01-08
    Description: Geochemical patterns in the environment are always the result of certain processes. Therefore, it is essential to decipher a process to properly evaluate the environmental role and potential of chemical elements/compounds. This allows the distinction between natural and anthropogenic influence on elemental concentrations. However, if the compositional nature of geochemical data is neglected, erroneous or misleading conclusions regarding the processes involved are probable. In this study the reconstruction of depositional environments and processes through the Holocene in two sediment cores obtained from submerged sinkholes located on the island of Mljet, Croatia, was performed by taking into account the compositional nature of geochemical, mineralogical and grainsize data. Problems involving compositional data are always multivariate; for example, the concentration of a single element does not carry any interpretative information, as only the ratios between elements do. This has led to the discovery of a large number of geochemical proxies based on elemental ratios, which describe certain environmental conditions and processes involved. Nevertheless, some proxies have been found to be restricted to only some specific environments, thus preventing them from being used in general; therefore, some kind of relation between different proxies is necessary to obtain final conclusions. However, when using simple elemental ratios, those correlations cannot be obtained due to the nature of compositional data. With a sequential binary partition of a compositional vector, orthonormal log ratio (olr) coordinates (proxies) can be constructed. When based on expert knowledge, those proxies fully acknowledge the geochemical properties of the chosen elements with one major difference - that the correlation between newly obtained variables is mathematically well grounded. As a result, the final conclusion is more accurate. In this research, geochemical proxies obtained as a representation in olr coordinates of the elements that are enriched compared to the local soil were used to perform principal component analyses. It helped to unravel the evolution of sedimentary environments. Mineralogical (XRD and heavy mineral data) and grain size analyses supported the conclusions obtained based solely on geochemical data. Furthermore, data analysis suggests that the proxies for redox conditions described in the literature should be used with caution, as their use is somewhat limited.
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  • 29
    Publication Date: 2021-01-08
    Description: New antifungals are increasingly needed due to the emergence of resistant fungal strains. Traditional antifungal assays are laborious and require significant amounts of samples. The present work presents a new proposal to evaluate antifungal activity and antagonism among fungal species, based on experiments of fungal culture and co-culture, 1H NMR profile of fungal culture extracts and chemometrics. In order to develop the work, six axenic cultures of fungi that infested fruits (Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora, and C. gloeosporioides), and co-cultures of all possible combination among them were performed (totalizing 63 experiments). All fungal extracts were evaluated by 1H NMR followed by Principal Component Analyses (PCA) in order to determine spectral dissimilarity among the extracts. Results showed that 1H NMR data evaluated by PCA were capable to predict both antagonism and antifungal activity. Traditional antifungal in vitro assays of active and inactive extracts were also performed in order to prove the prediction made by PCA. The obtained data showed that the approach is an outstanding tool to simultaneously obtain and evaluate bioactive compounds because: it was able to predict the activity of five different extracts in a collection of sixty-three, which would be much more difficult and time consuming if applied randomly; most important antifungal extracts are indicated by PCA; hundreds of traditional in vitro assays are avoid; and, the method is very time and money saving.
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  • 30
    Publication Date: 2021-01-08
    Description: Highlights • Total modeled carbon cycling at disturbed sites is lower than at reference sites. • Projected microbial loop functioning is reduced 26 years after sediment disturbance. • Estimated faunal respiration has recovered from sediment disturbance. • Estimated microbial respiration has not recovered from the sediment disturbance. Abstract Due to the predicted future demand for critical metals, abyssal plains covered with polymetallic nodules are currently being prospected for deep-seabed mining. Deep-seabed mining will lead to significant sediment disturbance over large spatial scales and for extended periods of time. The environmental impact of a small-scale sediment disturbance was studied during the ‘DISturbance and reCOLonization’ (DISCOL) experiment in the Peru Basin in 1989 when 10.8 km2 of seafloor were ploughed with a plough harrow. Here, we present a detailed description of carbon-based food-web models constructed from various datasets collected in 2015, 26 years after the experiment. Detailed observations of the benthic food web were made at three distinct sites: inside 26-year old plough tracks (IPT, subjected to direct impact from ploughing), outside the plough tracks (OPT, exposed to settling of resuspended sediment), and at reference sites (REF, no impact). The observations were used to develop highly-resolved food-web models for each site that quantified the carbon (C) fluxes between biotic (ranging from prokaryotes to various functional groups in meio-, macro-, and megafauna) and abiotic (e.g. detritus) compartments. The model outputs were used to estimate total system throughput, i.e., the sum of all C flows in the food web (the ‘ecological size’ of the system), and microbial loop functioning, i.e., the C-cycling through the prokaryotic compartment for each site. Both the estimated total system throughput and the microbial loop cycling were significantly reduced (by 16% and 35%, respectively) inside the plough tracks compared to the other two sites. Site differences in modelled faunal respiration varied among the different faunal compartments. Overall, modelled faunal respiration appeared to have recovered to, or exceeded reference values after 26-years. The model results indicate that food-web functioning, and especially the microbial loop, have not recovered from the disturbance that was inflicted on the abyssal site 26 years ago.
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  • 31
    Publication Date: 2021-01-08
    Description: Highlights: • Crystal structure of the malaria parasite lipocalin • Comparative analysis of lipocalin superfamily members in alveolate genomes • Localization of PfLipocalin to the parasitophorous vacuole and food vacuole • Reverse genetics reveal PfLipocalin function in oxidative damage control Summary: Proteins of the lipocalin family are known to bind small hydrophobic ligands and are involved in various physiological processes ranging from lipid transport to oxidative stress responses. The genome of the malaria parasite Plasmodium falciparum contains a single protein PF3D7_0925900 with a lipocalin signature. Using crystallography and small-angle X-ray scattering, we show that the protein has a tetrameric structure of typical lipocalin monomers; hence we name it P. falciparum lipocalin (PfLCN). We show that PfLCN is expressed in the intraerythrocytic stages of the parasite and localizes to the parasitophorous and food vacuoles. Conditional knockdown of PfLCN impairs parasite development, which can be rescued by treatment with the radical scavenger Trolox or by temporal inhibition of hemoglobin digestion. This suggests a key function of PfLCN in counteracting oxidative stress-induced cell damage during multiplication of parasites within erythrocytes.
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  • 32
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    Elsevier
    In:  Planetary and Space Science, 190 . Art.-Nr.: 105023.
    Publication Date: 2021-01-08
    Description: In a short note of 1998 Davankov questioned the generally accepted notion that the unique features of the planet Earth, namely, the presence of life and atmospheric oxygen are inseparably bonded as a case and effect. Indeed, photosynthesis in terrestrial and aquatic phototrophs simultaneously produces oxygen and carbohydrates in almost equal amounts. Since the degradation of organic matter through burning or rotting also consumes an equivalent amount of oxygen, the total masses of oxygen and organic material must always remain comparable. This correlation for Earth appears to be drastically distorted in favor of oxygen, thus disproving the still widespread delusion of biogenic origin of atmospheric oxygen. Instead, by analyzing more recent data on the balance between oxygen and organics we arrive at the conclusion that radiolysis of water vapors with the preferential dissipation of hydrogen to space, most probably, was the major source of the free and oxidation-spent oxygen. The present review tries to illuminate the most important uncertainties that still remain to be clarified before an interdisciplinary and scientifically-balanced picture on the evolution of Earth gets settled.
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  • 33
    Publication Date: 2021-01-08
    Description: Carnivorous gelatinous zooplankton (GZ) can be very abundant in marine ecosystems around the globe and exert considerable predation pressures on micro- to macrozooplankton as well as larval and juvenile fish. As these species are in many cases intermediate and top predators, their biomass can be easily evaluated, which could indicate overall food web stability or alteration. There is growing concern worldwide about increasing abundances of GZ species and consequently negative impacts on food webs and human coastal zones activities. In this paper, I present a case study from a shallow Danish cove and a long-term record spanning 29 years (1991–2019) obtained by unconventional means. Jellyfish were collected using horizontal sub-surface net tows in August and September during a summer student class. Sea surface temperature and chlorophyll a concentration were measured in parallel. No clear long-term trend in jellyfish biomass could be seen, whereas the values were highly variable from year to year. This is in contrast with other published Scandinavian long-term GZ time series. Sea surface temperature and chlorophyll a concentration had significant (and interactive) effects on the jellyfish biomass in Kertinge Nor. The scarcity and shortness of GZ long-term series do not allow solid conclusions in most marine ecosystems, so it becomes clear that publishing time series (even with small spatial extent) can contribute to improve the perception of interannual GZ population developments. Thus, I strongly recommend extending monitoring activities, explicitly including GZ taxa, in as many marine ecosystems as feasible.
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  • 34
    Publication Date: 2021-01-08
    Description: This paper describes methods of obtaining improved estimates of long-term sea level trends for the British Isles. This is achieved by lengthening the sea level records where possible, then removing known sources of variability, and then further adjusting for datum errors that are revealed by the previous processes after verification using metadata from archived sources. Local sea level variability is accounted for using a tide and surge model. Far field variability is accounted for using a “common mode”. This combination reduces the residual variability seen at tide gauges around the coast of the British Isles to the point that a number of previously unrecognised steps in individual records become apparent, permitting a higher level of quality control to be applied. A comprehensive data archaeology exercise was carried out which showed that these step-like errors are mostly coincident with recorded site-specific changes in instrumentation, and that in many cases the periodic tide gauge calibration records can be used to quantify these steps. A smaller number of steps are confirmed by “buddy-checking” against neighbouring tide gauges. After accounting for the observed steps, using levelling information where possible and an empirical fit otherwise, the records become significantly more consistent. The steps are not found to make a large difference to the trend and acceleration observed in UK sea level overall, but their correction results in much more consistent estimates of first order (Sea Level Rise) and second order (Sea Level Acceleration) trends over this 60-year period. We find a mean rate of sea level rise of 2.39 ± 0.27 mm yr−1, and an acceleration of 0.058 ± 0.030 mm yr−2 between Jan. 1958 and Dec. 2018. The cleaner dataset also permits us to show more clearly that the variability other than that derived from local meteorology is indeed consistent around the UK, and relates to sea level changes along the eastern boundary of the North Atlantic.
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  • 35
    Publication Date: 2021-01-08
    Description: Fucoxanthin is the major abundant xanthophyll in macro- and micro-algae as a component of photosynthetic light-harvesting complexes for photosynthesis and photoprotection. Nowadays, widespread application of fucoxanthin in food industry, pharmaceutical, and medical purposes are increasing. The current issue attracts the attention of researchers for producing of carotenoid from its natural resources, especially algae. Againts macroalgae, microalgae have rapid growth with the same source of food. In addition, they can grow from variety of situations and environmental conditions to produce a specific biochemical product. Microalgae, e.g. Tisochrysis lutea (T. lutea), can be cultivated under controlled conditions, low cost, and higher concentration of fucoxanthin. This review presents some of nutraceutical effects of fucoxanthin for human health and then, in particular, recent biotechnological developments in bioprocessing of this valuable product including, cultivation, harvesting, extraction, and purification, from T. lutea.
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  • 36
    Publication Date: 2021-01-08
    Description: Pretreatment of microalgal biomass possessing rigid cell wall is a critical step for enhancing the efficiency of microalgal biorefinery. However, the conventional pretreatment processes suffer the drawbacks of complex processing steps, long processing time, low conversion efficiency and high processing costs. This significantly hinders the industrial applicability of microalgal biorefinery. The innovative electricity-aid pretreatment techniques serve as a promising processing tool to extensively enhance the release of intracellular substances from microalgae. In this review, application of electric field-based techniques and recent advances of using electrical pretreatments on microalgae cell focusing on pulsed electric field, electrolysis, high voltage electrical discharges and moderate electric field are reviewed. In addition, the emerging techniques integrating electrolysis with liquid biphasic flotation process as promising downstream approach is discussed. This review delivers broad knowledge of the present significance of the application of these methods focusing on the development of electric assisted biomolecules extraction from microalgae.
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  • 37
    Publication Date: 2021-01-08
    Description: Coastal communities, knowingly or otherwise, rely on seagrasses for their livelihood, recreation and food source, among other services. However, despite its importance, seagrasses are not receiving similar attentions with its adjacent ecosystems, the mangroves, and coral reefs. Because of their role in climate change mitigation, seagrasses along with mangroves and salt marshes (the blue carbon ecosystems) are gaining attention recently. This preliminary study investigates the perception of coastal communities in Eastern Samar, Philippines on seagrasses. The results of the survey show that there is a level of high awareness among respondents to seagrass ecosystem services. Despite that, however, utilization remains low. The level of awareness varies on the type of ecosystem service; for instance, locals have a high awareness of provisioning services while low awareness of cultural services. The survey also included the perceived threats to seagrasses, where damages caused by natural disturbances are identified as the most concerning threat. This may be due to the geographic location of the sites that are frequented by typhoons. The perception potentially indicates depleted resources brought about by mismanagement or overlooking this natural resource. The results provide a more contextualized understanding on how local communities are aware of and interacting with, or the lack thereof, seagrass ecosystems. This could aid local government units (LGUs) and conservation groups in the area to craft practicable and sustainable management plans and threat-specific solutions.
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  • 38
    Publication Date: 2021-01-08
    Description: Simulating complex physical systems often involves solving partial differential equations (PDEs) with some closures due to the presence of multi-scale physics that cannot be fully resolved. Therefore, reliable and accurate closure models for unresolved physics remains an important requirement for many computational physics problems, e.g., turbulence simulation. Recently, several researchers have adopted generative adversarial networks (GANs), a novel paradigm of training machine learning models, to generate solutions of PDEs-governed complex systems without having to numerically solve these PDEs. However, GANs are known to be difficult in training and likely to converge to local minima, where the generated samples do not capture the true statistics of the training data. In this work, we present a statistical constrained generative adversarial network by enforcing constraints of covariance from the training data, which results in an improved machine-learning-based emulator to capture the statistics of the training data generated by solving fully resolved PDEs. We show that such a statistical regularization leads to better performance compared to standard GANs, measured by (1) the constrained model's ability to more faithfully emulate certain physical properties of the system and (2) the significantly reduced (by up to 80%) training time to reach the solution. We exemplify this approach on the Rayleigh-Benard convection, a turbulent flow system that is an idealized model of the Earth's atmosphere. With the growth of high-fidelity simulation databases of physical systems, this work suggests great potential for being an alternative to the explicit modeling of closures or parameterizations for unresolved physics, which are known to be a major source of uncertainty in simulating multi-scale physical systems, e.g., turbulence or Earth's climate.
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  • 39
    Publication Date: 2021-01-08
    Description: Microalgae are a rich source of natural bioactive compounds, e.g. astaxanthin, β-carotene, lutein, and fatty acids (FAs), that are currently in high demand in the market. Conventional extraction methods often produce adverse effects on some of these compounds. Replacing conventional extraction methods with more efficient advanced green technologies that offer greater extracts purity and low environmental impact is therefore a challenging and sought-for target. This review is a comprehensive overview of supercritical fluid (SCF) extraction processes, including the latest research on the extraction of bioactive compounds from microalgae biomass and their benefits on human health. In addition, the role of key operating parameters on the selectivity of various compounds is discussed. This study provides useful knowledge that can productively contribute to the future development of SCF-based extraction technologies on an industrial scale.
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  • 40
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    Elsevier
    In:  Trends in Genetics, 36 (6). pp. 395-402.
    Publication Date: 2021-01-08
    Description: Aging entails an irreversible deceleration of physiological processes, altered metabolic activities, and a decline of the integrity of tissues, organs, and organ systems. The accumulation of alterations in the genetic and epigenetic spaces has been proposed as an explanation for aging. They result, at least in part, from DNA replication and chromosome segregation errors due to cell division during development, growth, renewal, and repair. Such deleterious alterations, including epigenetic drift, irreversibly accumulate in a stepwise, ratchet-like manner and reduce cellular fitness, similar to the process known as Muller’s ratchet. Here, we revisit the Muller’s ratchet principle applied to the aging of somatic cell populations and discuss the implications for understanding the origins of senescence, frailty, and morbidity.
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  • 41
    Publication Date: 2021-01-08
    Description: Natural cycles in the seawater partial pressure of carbon dioxide (CO2) in the Gulf of Maine, which vary in surface waters from ~250 to 550 µatm seasonally, provide an opportunity to observe how the life cycle and phenology of the shelled pteropod Limacina retroversa responds to changing food, temperature and carbonate chemistry conditions. Distributional, hydrographic, and physiological sampling suggest that pteropod populations are located in the upper portion of the water column (0–150 m) with a maximum abundance above 50 m. Gene expression and shell condition measurements show that the population already experiences biomineralization stress in the winter months when measured aragonite saturation state was at a seasonal low (though slightly oversaturated), reinforcing the usefulness of this organism as a bio-indicator for pelagic ecosystem response to ocean acidification. There appear to be two reproductive events per year with one pulse timed to coincide with the spring bloom, the period with highest respiration rate, fluorescence, and pH, and a second more extended pulse in the late summer and fall when saturation states remain high and fluorescence begins to decline. During the fall there is transcriptomic evidence of lipid storage for overwintering, allowing the second generation to survive the period of low food and aragonite saturation state. Based on these observations we predict that in the future pteropods will likely be most vulnerable to changing CO2 regionally during the fall reproductive event when CO2 concentration already naturally rises and when there is the added stress of generating lipid stores.
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  • 42
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    Elsevier
    In:  Computers & Geosciences, 139 . Art.Nr. 104482.
    Publication Date: 2021-01-08
    Description: We use numerical simulations on petrographically characterized thin-section images to predict three-dimensional elastic compressibility of sandstones. We predict two key statistics of compressibility curves measured under uniaxial boundary conditions in a laboratory - i) minimum compressibility at 1500 psi or 10 MPa depletion stress, and ii) maximum compressibility. A new Digital Rock workflow was developed for predicting compressibility based on the simulation of stress field using a segmented two-dimensional thin-section image. We also propose linear and non-linear relationships of log base 10 (compressibility) with in-situ porosity that can be used for compressibility prediction in the absence of laboratory measurements or two-dimensional images. Based on the results of application of the proposed relationships on samples from different fields with laboratory measurements, we conclude that the best prediction for minimum compressibility is obtained using the Digital Rock workflow and the best prediction for maximum compressibility is obtained using the proposed non-linear relationship using in-situ porosity. The range of compressibility values given by the difference between maximum and minimum compressibility predicted using the proposed methods can be used in making better informed economic decisions in field development planning.
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  • 43
    Publication Date: 2021-01-08
    Description: Pteropods are holopelagic marine snails and slugs that are of particular interest to science due to their role in marine food webs, global carbon cycle and their potential sensitivity to ocean change. Due to their pelagic, often exclusively offshore occurrence, samples are difficult to obtain, resulting in a lack of knowledge about their physiology, ecology, anatomy, geographical ranges, phylogenetic relationships and reproductive biology. Despite a recent increase in interest surrounding pteropod taxonomy, many evolutionary uncertainties remain due to limited taxon sampling and inavailability of molecular vouchers, in particular for the lesser investigated groups Pseudothecosomata and Gymnosomata. The Northwest Pacific Ocean is one of the least investigated areas for pteropods and in the adjacent semi-enclosed Sea of Okhotsk basin, current knowledge is restricted to the epipelagic zone. We summarize results from plankton hauls (from up to 5900 m depth) conducted during the joint German/Russian SokhoBio and KuramBio II cruises to the Sea of Okhotsk and the Kuril-Kamchatka-Trench region. This study presents an integrative taxonomic overview of six pteropod species identified by detailed morphological methods, including serial semithin sectioning, µCT and SEM scanning supported by multimarker (COI, 28S, and H3) genetic barcoding. We found four species of Gymnosomata slugs (Clione limacina, Clione okhotensis, Notobranchaea grandis and Thliptodon sp.), three species of Euthecosomata snails (Limacina helicina and two genetically delimited Clio spp.) and one shelled pseudothecosome species that is probably new to science (Peracle n. sp.). Multilocus phylogenetic analyses support monophyly of major traditional groups such as Pteropoda, Thecosomata, Pseudothecosomata and Gymnosomata. Micro-CT scanning was applied for the first time on pteropod soft bodies, allowing direct comparison between detailed anatomical peculiarities and molecular barcodes of the respective species. Furthermore, taxonomic positions, geographical ranges and potential dispersal barriers are discussed, with implications for future biodiversity comparisons. This study serves as a solid foundation for monitoring pteropods in a changing ocean.
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  • 44
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    Elsevier
    In:  European Journal of Medicinal Chemistry, 187 . Art.Nr. 111909.
    Publication Date: 2021-01-08
    Description: Hybrid chemical compounds formed by conjugation of two or more bioactive molecules have shown wide variety of applications in biology, microelectronics as well as material sciences. In particular, the conjugates of steroid framework are known to have broad biological activity profile due to their ability to penetrate the biomembranes and bind to specific hormone receptors. Among the various conjugates of steroids, Steroid Amino Acid Conjugates (SAACs) are attractive because of the possibility of fine tuning of the amphiphilicity with position, orientation and nature of amino acids. The structural details, applications, mechanistic insights and their diverse pharmacological as well as other physicochemical properties of several SAACs are summarized in the present review. This review provides better insight for medicinal chemists to design and explore such novel conjugates which can be used as lead structures in the future drug discovery or as probes to understand the complex biological system.
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  • 45
    Publication Date: 2021-01-08
    Description: Cell disruption is regarded as an indispensable pretreatment step before the extraction of microalgae with biomineralized cell walls. Here, two typical microalgae—diatom Chaetoceros gracilis (C. gracilis) and coccolithophore Pleurochrysis carterae (P. carterae)—covered by “hard” biomineralized cell walls were used as starting materials for lipid extraction using liquefied dimethyl ether (DME) without any pretreatment such as drying or cell disruption. The liquefied DME extraction experiments were performed at 25 °C and 0.59 MPa using a semi-continuous, flow-type system. The results of the yield, elemental composition, molecular weight distribution, fatty acid composition, and trace element composition indicated that the performance of liquefied DME extraction was similar to that of Bligh–Dyer extraction and better than that of hexane Soxhlet extraction, despite the latter two methods requiring pre-drying and cell disruption processes. It was also proven that the cell wall of microalgae would not affect lipid extraction of liquefied DME, thereby the liquefied DME extraction method is suitable for extracting lipids from microalgae with biomineralized cell walls. Besides, the lipids extracted by liquefied DME can be further used for biodiesel production.
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  • 46
    Publication Date: 2021-01-08
    Description: In recent years, metagenomic strategies have been widely used to isolate and identify new enzymes from uncultivable components of microbial communities. Among these enzymes, various lipases have been obtained from metagenomic libraries from different environments and characterized. Although many of these lipases have characteristics that could make them interesting for application in biocatalysis, relatively little work has been done to evaluate their potential to catalyze industrially important reactions. In the present article, we highlight the latest research on lipases obtained through metagenomic tools, focusing on studies of activity and stability and investigations of application in biocatalysis. We also discuss the challenges of metagenomic approaches for the bioprospecting of new lipases.
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  • 47
    Publication Date: 2021-01-08
    Description: There are no effective therapies for achondroplasia. An open-label study suggested that vosoritide administration might increase growth velocity in children with achondroplasia. This phase 3 trial was designed to further assess these preliminary findings. Methods This randomised, double-blind, phase 3, placebo-controlled, multicentre trial compared once-daily subcutaneous administration of vosoritide with placebo in children with achondroplasia. The trial was done in hospitals at 24 sites in seven countries (Australia, Germany, Japan, Spain, Turkey, the USA, and the UK). Eligible patients had a clinical diagnosis of achondroplasia, were ambulatory, had participated for 6 months in a baseline growth study and were aged 5 to less than 18 years at enrolment. Randomisation was done by means of a voice or web-response system, stratified according to sex and Tanner stage. Participants, investigators, and trial sponsor were masked to group assignment. Participants received either vosoritide 15·0 μg/kg or placebo, as allocated, for the duration of the 52-week treatment period administered by daily subcutaneous injections in their homes by trained caregivers. The primary endpoint was change from baseline in mean annualised growth velocity at 52 weeks in treated patients as compared with controls. All randomly assigned patients were included in the efficacy analyses (n=121). All patients who received one dose of vosoritide or placebo (n=121) were included in the safety analyses. The trial is complete and is registered, with EudraCT, number, 2015-003836-11. Findings All participants were recruited from Dec 12, 2016, to Nov 7, 2018, with 60 assigned to receive vosoritide and 61 to receive placebo. Of 124 patients screened for eligibility, 121 patients were randomly assigned, and 119 patients completed the 52-week trial. The adjusted mean difference in annualised growth velocity between patients in the vosoritide group and placebo group was 1·57 cm/year in favour of vosoritide (95% CI [1·22–1·93]; two-sided p〈0·0001). A total of 119 patients had at least one adverse event; vosoritide group, 59 (98%), and placebo group, 60 (98%). None of the serious adverse events were considered to be treatment related and no deaths occurred. Interpretation Vosoritide is an effective treatment to increase growth in children with achondroplasia. It is not known whether final adult height will be increased, or what the harms of long-term therapy might be.
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  • 48
    Publication Date: 2021-01-08
    Description: Biofouling is a challenge in global sea-based salmon farming. Norway's salmon-growing industry relies primarily on copper-based antifouling coatings. However, copper is an increasingly recognised environmental hazard, and there is a need to develop alternative antifouling products to prevent biofouling in marine aquaculture. Using field experiments, this study compared the efficacy of six novel antifouling coatings for fish farm nets (two with reduced copper content, three with alternative biocides and one biocide-free coating) against a popular commercial copper coating and uncoated samples. The performance of one of the new coatings with lower copper content was more similar to the commercial copper control while the rest were colonised by biofouling faster and/or at higher abundances. However, none of the tested products were able to prevent biofouling entirely, underlining the importance of the search for alternative and improved antifouling technologies.
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  • 49
    Publication Date: 2021-01-08
    Description: Seagrass meadows are important productive ecosystems; during the Summer period in touristic beaches, such as those located in the high Adriatic coast, seagrasses are removed from the shoreline and disposed in landfill. This study investigated anaerobic digestion potential of beach-cast seagrass wrack, considering the physicochemical characteristics of the substrate and analysing heavy metal presence in the digestate, with the aim of transporting the material to local wastewater treatment plants to increase biogas yield from excess sludge anaerobic digestion. The methane production obtained from seagrass wrack was compared with three theoretical models. Seagrass wrack had a good methane potential of 103.1–262.3 NmL CH4/g Volatile Solids (VS), depending on substrate humidity and applied inoculum-to-substrate ratio. Predictive models, based on elemental composition and proximate analysis, successfully estimated methane yields; heavy metal concentration in digestate was low, boosting for digestate agricultural reuse. A simplified energy analysis revealed that transport to local wastewater treatment plants and use in anaerobic digestion process would provide up to 245,000 Nm3/y of methane, with an estimated economic income of 33,500–193,300 €/y, considering local seagrass production (1,465–8,454 t/y). Actual yearly costs sustained by beach management company for landfill disposal was about 117,200–676,320 €/y. Seagrass reuse in local digesters would compensate for the lack of excess sludge encountered during the cold season, allowing the digester to operate more continuously, increasing biogas production and reducing plant energy need.
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  • 50
    Publication Date: 2021-01-08
    Description: Six new compounds including two azaphilones, lunatoic acids D–E (1, 2), three isocoumarins, lunatinins B–D (3-5), and one α-pyrone derivative, lunatinin E (6), as well as four known ones, lunatoic acid A (7), lunatinin (8), penicipyran D (9) and chaetoquadrin F (10) were isolated from the rice medium of the gut fungus Paraphaeosphaeria sp. XZD2-1. Their planar structures were elucidated by analysis of 1D and 2D NMR as well as HRESIMS spectroscopic data. The absolute configurations of compounds 1-3 were assigned by experimental and calculated ECD data and an ester hydrolysis reaction. Compounds 4 and 5 were a pair of enantiomeric excess mixture. Structure of compound 6 was further confirmed by comparing optical rotation with known compounds according to literature. The antimicrobial activity and cytotoxicity of compounds 1-10 were examined.
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  • 51
    Publication Date: 2021-01-08
    Description: As the frequency and intensity of storms increase, a growing need exists for resilient shore protection techniques that have both environmental and economic benefits. In addition to producing seafood, aquaculture farms may also provide coastal protection benefits either alone or with other nature-based structures. In this paper, a generalized three-layer frequency dependent theoretical model is derived for random wave attenuation due to presence of biomass within the water column. The biomass can be characterized as submerged, emerged, suspended and floating canopies that can consist of natural aquatic vegetation with potential aquaculture systems of kelp or mussels. The present analytical solutions can reduce to the solutions by Mendez and Losada (2004), Chen and Zhao (2012) and Jacobsen et al. (2019) for submerged rigid aquatic vegetation. The present theoretical model incorporates the motion of these canopies using a cantilever-beam model for slender components and a buoy-on-rope model for elements with concentrated mass and buoyancy. Analytical results are compared with existing laboratory and field datasets for submerged and suspended canopies. The theoretical model was then used (in a case study at a field site in Northeastern US) to investigate the capacity of suspended mussel farms with submerged aquatic vegetation (SAV) to dissipate wave energy during a recent storm event. Compared to a dense SAV meadow in shallower water, the suspended aquaculture farms more effectively attenuate random waves with a smaller peak period and the higher frequency components of wave spectrum. The performance of suspended aquaculture farms is less affected by water level changes due to tides, surge and sea level rise, while the wave attenuation performance of SAV decreases with increasing water level due to decreased wave motion near the sea bed. Incorporating suspended aquaculture farms offshore significantly enhance the coastal protection effectiveness of SAV-based living shorelines and extend the wave attenuation capacity over a wider wave period and water level range. The combination of suspended aquaculture farms and traditional living shorelines provides a more effective nature-based coastal defense strategy than the traditional living shorelines alone.
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  • 52
    Publication Date: 2021-01-08
    Description: Application of polyester-degrading enzymes should be considered as an eco-friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. Many hydrolases from several fungi and bacteria have been discovered and successfully evaluated for their activity towards different aliphatic polyesters (PHA, PBS, PBSA, PCL, PLA), aromatic polyesters (PET, PBT, PMT) as well as their co-polyesters (PBST, PBAT, PBSTIL). This revision gives an up-to-date overview on the main biochemical features and biotechnological applications of those reported enzymes which are able to degrade polyester-based plastics, including different microbial polyester depolymerases, esterases, cutinase-like enzymes and lipases. Summarized information includes available protein sequences with the corresponding accession numbers deposited in NCBI server, 3D resolved structures, and data about optimal conditions for enzymatic activity and stability of many of these microbial enzymes that would be helpful for researchers in this topic. Although screening and identification of new native polyester hydrolases from microbial sources is undeniable according to literature, we briefly highlight the importance of the design of improved enzymes towards recalcitrant aromatic polyesters through different approaches that include site-directed mutagenesis and surface protein engineering.
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  • 53
    Publication Date: 2021-01-08
    Description: The subpopulation of Atlantic cod, Gadus morhua, in the eastern part of the Baltic Sea has experienced a significant increase in infections with anisakid nematode larvae of the species Contracaecum osculatum sensu lato (s.l.) since the year 2000. The life cycle of the parasite includes seals and especially the grey seal, Halichoerus grypus, as final hosts, carrying the adult nematodes in the stomach, crustaceans (copepods, amphipods) as first intermediate hosts and various fish species (clupeids, sandeel) including cod as second intermediate/paratenic hosts. Cod with a body length below 28 cm are generally non-infected but experience increasing infection levels when they switch to a piscine diet (infected intermediate/paratenic hosts). We present an overall frequency distribution analysis of worms in 166 cod (body length 30–49 cm) collected in the spawning area over the last 5 years. It shows a fit to the negative binomial distribution, a prevalence of infection of 89.8%, a mean intensity of 29.3 parasites per fish (range 1–377) and a variance/mean ratio of 59.2 (≫1), indicating overdispersion. We present measurements of the adult Contracaecum osculatum (s.l.) specimens in the seal stomach and show that the parasites reach a maximum length of 6.6 cm (females) and 5.8 cm (males). L3s in sprat have a total length from 1to 11 mm whereas the larvae in cod liver are 3–27 mm. A decreasing mean worm length associated with high worm densities in cod (number of nematodes per liver) was recorded. Possible explanations might include timing of feeding on infected intermediate/paratenic hosts, intraspecific competition (crowding) between larvae in cod and host responses (indicated by a significant antibody production in cod against C. osculatum (s.l.) antigens). A significant negative correlation between infection intensity and muscle mass of cod was found, suggesting parasite-induced down-regulation of growth factors in cod.
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  • 54
    Publication Date: 2021-01-08
    Description: Despite their critical role as the main energy pathway between phytoplankton and fish, the functional complexity of zooplankton is typically poorly resolved in marine ecosystem models. Trait-based approaches—where zooplankton are represented with functional traits such as body size—could help improve the resolution of zooplankton in marine ecosystem models and their role in trophic transfer and carbon sequestration. Here, we present the Zooplankton Model of Size Spectra version 2 (ZooMSSv2), a functional size-spectrum model that resolves nine major zooplankton functional groups (heterotrophic flagellates, heterotrophic ciliates, larvaceans, omnivorous copepods, carnivorous copepods, chaetognaths, euphausiids, salps and jellyfish). Each group is represented by the functional traits of body size, size-based feeding characteristics and carbon content. The model is run globally at 5° resolution to steady-state using long-term average temperature and chlorophyll a for each grid-cell. Zooplankton community composition emerges based on the relative fitness of the different groups. Emergent steady-state patterns of global zooplankton abundance, biomass and growth rates agree well with empirical data, and the model is robust to changes in the boundary conditions of the zooplankton. We use the model to consider the role of the zooplankton groups in supporting higher trophic levels, by exploring the sensitivity of steady-state fish biomass to the removal of individual zooplankton groups across the global ocean. Our model shows zooplankton play a key role in supporting fish biomass in the global ocean. For example, the removal of euphausiids or omnivorous copepods caused fish biomass to decrease by up to 80%. By contrast, the removal of carnivorous copepods caused fish biomass to increase by up to 75%. Our results suggest that including zooplankton complexity in ecosystem models could be key to better understanding the distribution of fish biomass and trophic efficiency across the global ocean.
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  • 55
    Publication Date: 2021-01-08
    Description: Mitigating human contributions to climate change is a highly debated topic, as it becomes evident that many nations do not adhere to optional reductions in global emission. Substantial research is taking place into negative carbon technologies that actively reduce the amount of atmospheric carbon dioxide (CO2) via greenhouse gas removal (GGR). Various GGR methods have been proposed, from reforestation to ocean fertilisation. This article discusses advantages of an approach based on enhanced input of tephra to the ocean, to increase the drawdown of atmospheric CO2. Natural addition of tephra to the ocean results in preservation of enhanced organic matter in sediment. Hence, augmenting its delivery should raise the level of sequestration. Calculations indicate that offshore tephra addition could sequester 2750 tonnes of CO2 per 50,000 tonnes of ash delivered (a typical bulk carrier’s capacity). The cost is estimated as ∼$55 per tonne of CO2 sequestered and is an order of magnitude cheaper than many proposed GGR technologies. Further advantages include: tephra addition is simply an augmentation of a natural Earth process, it is a low technology approach that requires few developments, and it may sequester carbon for thousands of years. Hence, offshore tephra addition warrants further investigation to assess its viability.
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  • 56
    Publication Date: 2021-01-08
    Description: Used during an oil spill to minimise the formation of an oil slick, dispersants have negative biological effects on marine model organisms. However, no study has investigated the impacts of dispersants on adult sponge individuals. Here, we examine the effects of water accommodated oil fraction (WAF - oil in seawater), chemically enhanced WAF (CEWAF - oil and dispersant in seawater) and Benzo[A]Pyrene on sponge Halichondria panicea at physiological and molecular levels. Sponge clearance rate decreased sharply when exposed to WAF and CEWAF but the oil loading at which the clearance rate was reduced by 50% (ED50) was 39-fold lower in CEWAF than in WAF. Transcriptomic analysis revealed a homogenous molecular response with the greatest number of differentially expressed genes identified in CEWAF samples (1,461 genes). Specifically, genes involved in stress responses were up-regulated. This study presents evidence that the use of dispersants should be considered carefully in areas where sponges are present.
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  • 57
    Publication Date: 2021-01-08
    Description: This study evaluated the application of a Halobacteriovorax isolated from water of the Adriatic Sea (Italy) in controlling V. parahaemolyticus in mussels (Mytilus galloprovincialis). Two 72 h laboratory-scale V. parahaemolyticus decontamination experiments of mussels were performed. The test microcosm of experiment 1 was prepared using predator/prey free mussels experimentally contaminated with Halobacteriovorax/V. parahaemolyticus at a ratio of 103 PFU/105 CFU per ml, while that of experiment 2 using mussels naturally harbouring Halobacteriovorax that were experimentally contaminated with 105 CFU per ml of V. parahaemolyticus. For experiment 1, was also tested a control microcosm only contaminated with 105 CFU per ml of V. parahaemolyticus.. Double layer agar plating and pour plate techniques were used to enumerate Halobacteriovorax and V. parahaemolyticus, respectively. 16 S rRNA analysis was used to identify Halobacteriovorax. For both experiments in the test microcosm the concentration of prey remained at the same level as that experimentally added, i.e. 5 log for the entire analysis period. In experiment 1, V. parahaemolyticus counts in mussels were significantly lower in the test microcosm than the control with the maximum difference of 2.2 log at 24 h. Results demonstrate that Halobacteriovorax can modulate V. parahaemolyticus level in the mussels. The public impact of V. parahaemolyticus in bivalves is relevant and current decontamination processes are not always effective. Halobacteriovorax is a suitable candidate in the development of a biological approach to the purification of V. parahaemolyticus in mussels.
    Type: Article , PeerReviewed
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  • 58
    Publication Date: 2021-01-08
    Description: The ctenophore Mnemiopsis leidyi, ranking among the 100 most damaging bioinvaders in the world, is a major predator of zooplankton, known to alter the biodiversity and functioning of the ecosystems in which it has been introduced. This first survey on the trophic relationships of M. leidyi in a Mediterranean lagoon (Berre, South of France) was performed through stable isotope analyses. Carbon and nitrogen isotope composition was used (1) to determine the types of prey ingested by this ctenophore and (2) the influence of individual size on its isotope composition, and (3) to make assumptions to explore its trophic relationships with the native jellyfish Aurelia sp.. The two gelatinous species ingested mainly (79% to 97% of the diet) planktonic prey (copepods, cirriped nauplii, gastropod larvae and cladocerans), but also preyed upon benthic organisms (mainly harpacticoid copepods and the amphipod Monocorophium insidiosum) in lower proportions (2.5% to 21%). Size-related changes in M. leidyi diet were evidenced with an increase in trophic level and benthic prey consumption in the larger individuals. These two gelatinous organisms probably play an important and underestimated role in the benthic–pelagic​ coupling in coastal lagoons by transferring benthic organic matter to the pelagic food webs.
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  • 59
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    Unknown
    Elsevier
    In:  In: Green Sustainable Process for Chemical and Environmental Engineering and Science. , ed. by Inamuddin, Elsevier, Amsterdam, pp. 17-39, 23 pp. ISBN 978-0-12-817386-2
    Publication Date: 2020-05-08
    Description: Microalgal oils are considered an important source of industrially valuable oleochemicals with significant applications ranging from the energy to pharmaceutical sectors. Industrial production of microalgal oil is emerging rapidly; however, the high cost associated with downstream processes may constrain this process. Oils are accumulated intracellularly in oleaginous microalgae in the form of lipid droplets, which in turn require cell wall disruption followed by extraction in order to recover them. Disruption of the microalgal cell is very challenging owing to its distinctive features like high water content, hard cell wall, presence of algaenan, and sporopollenin like biopolymers that in turn create hurdles in efficient extraction of lipids. Various conventional pretreatment methods have been explored to rupture the cellular integrity of microalgal cells to enhance lipid extraction, and each method has certain advantages and disadvantages. Supercritical fluid extraction is the oldest technique for the extraction of valuable compounds from microalgae and is considered an alternative to conventional solvent extraction methods. It has several advantageous features such as being free from organic solvents (and their disposal), environment-friendly, and operating at a mild range of temperature (40–80°C). CO2 is considered to be an ideal supercritical fluid due to its non-toxic, non-flammable, and lipophilic nature. In this chapter, use of supercritical carbon dioxide extraction of lipids from microalgae is discussed and compared with other available lipid extraction methods.
    Type: Book chapter , NonPeerReviewed
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  • 60
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    Unknown
    Elsevier
    In:  In: Handbook of Algal Science, Technology and Medicine. , ed. by Konur, O. Elsevier, San Diego, pp. 675-696.
    Publication Date: 2020-04-08
    Description: The analysis of more than 400 papers found in the literature on Sargassum biosorption has shown the existence of more than 700 equilibrium entries corresponding to data at different temperature and pH conditions. The following ten single metals: Cd, Co, Cr(III, VI), Cu, Fe, Hg, La, Ni, Pb, and Zn, are the main focus of most of the equilibrium data (507) in more than half of the studied references. The studies reflecting the interaction of nine of these metals with Sargassum sp. is described and analyzed. The use of Langmuir equation, the effect of temperature and pH on sorption is critically reviewed. In addition, we also analyze all the data available on elemental chemical composition of native Sargassum. These data reflect the interaction of this kind of marine biomass with nine of the metals mentioned above, which are present in seawater worldwide.
    Type: Book chapter , NonPeerReviewed
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