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  • Phytochrome  (56)
  • Springer  (56)
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • Springer Nature
  • 2020-2022
  • 1975-1979  (56)
Collection
Keywords
Publisher
  • Springer  (56)
  • American Meteorological Society
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • Springer Nature
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Evidence of phytochrome involvement in the entrainment of the circadian rhythm of carbon dioxide metabolism in Bryophyllum (1978)
    Springer
    Planta 138 (1978), S. 271-278 
    Details
    ISSN: 1432-2048
    Keywords: Bryophyllum ; Circadian rhythm ; CO2 output ; Phytochrome ; Red light ; Rhythm (circadian)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rhythm of carbon dioxide output in Bryophyllum leaves was entrained on exposure to 0.25 h of white light every 24 h. Entrainment also occurred on similar exposure to monochromatic radiation in spectral bands centred at 660 nm and, to a lesser extent, at 730 nm, but a band centred at 450 nm was without effect. A “skeleton” irradiation programme comprising two 0.25-h exposures to white light per 24 h also entrained the rhythm when the intervening dark periods were either 7.5 h and 16 h, or 10.5 h and 13 h. The rhythm disappeared when the two exposures were separated by 11.5-h and 12-h dark periods. Regular 0.25-h exposures to red light separated by 11.75-h periods of darkness also resulted in loss of the rhythm. Red/far-red reversibility was observed in irradiation schedules having either one or two exposures to red light daily. In the latter case, far-red reversal of the effects of one of the exposures to red light resulted in entrainment of the rhythm by the other, instead of abolition of the rhythm. The occurrence of distinct red/far-red reversibility suggests strongly that phytochrome is the pigment involved in entrainment of this rhythm by cycles of light and darkness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386822
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  • 2
    Electronic Resource
    Electronic Resource
    Phytochrome and phosphotungstate-chromate-positive vesicles from Cucurbita pepo L. (1977)
    Springer
    Planta 133 (1977), S. 169-177 
    Details
    ISSN: 1432-2048
    Keywords: Cell fractionation ; Cucurbita ; Histochemical staining ; Phosphotungstate-chromate staining ; Phytochrome ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phosphotungstic acid-chromic acid (PTA-CrO3) stain, putatively specific for the plasma membrane of plants, has been used in an attempt to monitor the distribution of this membrane in a 20,000 x g particulate fraction from Cucurbita hypocotyl hooks. On discontinuous sucrose gradients, the relative distributions of the phytochrome and PTA-CrO3-positive vesicles present in this fraction appear to be correlated. When intact tissue is stained, however, other components, in addition to the plasma membrane, react positively to the stain. These components include prolamellar-body membranes, lipid droplets, and ribosomes. This lack of specificity calls into question the reliability of the technique for the unequivocal identification and accurate quantitation of plasma-membrane fragments in isolated particulate fractions. The present data do not, therefore, provide unambiguous evidence that phytochrome is associated with plasma membrane in tissue homogenates from Cucurbita.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391916
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of chlorophyll content on phytochrome measurements in turnip cotyledons (1977)
    Springer
    Planta 134 (1977), S. 11-16 
    Details
    ISSN: 1432-2048
    Keywords: Brassica ; Chlorophyll ; Cotyledons ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome determinations at 730/800 nm were performed on de-etiolated turnip (Brassica rapa L.) cotyledons in which chlorophyll (Chl) content had been reduced experimentally to varying degrees by pre-treatment with high temperature (HT), or transfer to 3% ethanol. The magnitude of detectable phytochrome depended on Chl content, showing a linear relationshop in vivo. The results were confirmed by an in vitro experiment where, however, the correlation was exponential. An attempt is made to illustrate phytochrome decay in continuous blue or red light after corretion for Chl interference. To overcome the possible objection that the higher Δ(ΔA) measured in continuous light after HT pre-treatment could be caused by a reduced rate of destruction, apparent re-synthesis following red light was measured after several hours of darkness during which time the HT effect was lost. Under these conditions HT pre-treated samples display a more realistic magnitude of apparent new synthesis and make correlation with a physiological response possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390087
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  • 4
    Electronic Resource
    Electronic Resource
    Radiation-induced appearance of phytochrome in soybean cell suspensions (1977)
    Springer
    Planta 134 (1977), S. 57-59 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture ; Clycine ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract No phytochrome can be detected spectrophotometrically in soybean (Glycine max (L.) Merr. cv. Mandarin) cell suspensions grown in darkness. White light from a tungsten filament lamp and a band of far-red light have been found to induce the appearance of phytochrome in them. Red light failed to bring about phytochrome increase and destroyed the phytochrome induced by far-red light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390095
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  • 5
    Electronic Resource
    Electronic Resource
    Chlorophyll interference with phytochrome measurement (1977)
    Springer
    Planta 135 (1977), S. 119-123 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Measurements of phytochrome by Δ (ΔA725–815 nm) were completely suppressed at chlorophyll concentrations of the order of 20–40 μg g-1 f.wt. in vivo and 37 μg cm-3 in vitro, and the readings were reduced by 50% at only 12 μg cm-3 in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with ΔA measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387159
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  • 6
    Electronic Resource
    Electronic Resource
    Photoreception and photoresponses in the radish hypocotyl (1977)
    Springer
    Planta 136 (1977), S. 125-129 
    Details
    ISSN: 1432-2048
    Keywords: Blue-light-response ; Elongation ; High-irradiance-response ; Photomorphogenesis ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In etiolated hypocotyls of Raphanus sativus L. the growth responses to continuous red, far-red and blue light have been distinguished on the bases of photoreceptive sites and regions of physiological response. Blue light appeared to retard a fairly mature stage of elongation, acting immediately and directly on the cells irradiated. Far-red light caused a marked inhibition of all stages of elongation after a lag period, and the stimulus could be transmitted from the hook region. The effect of red light was complex and consisted of one promotive and two inhibitory responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396187
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  • 7
    Electronic Resource
    Electronic Resource
    Action spectra for the inhibition of growth in radish hypocotyls (1977)
    Springer
    Planta 136 (1977), S. 131-134 
    Details
    ISSN: 1432-2048
    Keywords: Action-spectrum ; Blue-light photoreceptor ; High-irradiance-response ; Photomor-phogenesis ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In etiolated seedlings of Raphanus sativus L. the inhibition of hypocotyl elongation by continuous light showed a major bimodal peak of action in the red and far-red, and two minor peaks in the blue regions of the spectrum. It is argued that, under conditions of prolonged irradiation, phytochrome is the pigment controlling the inhibition of hypocotyl elongation by red and far-red light, but that its mode of action in far-red is different from that in red. A distinct pigment is postulated for blue light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396188
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  • 8
    Electronic Resource
    Electronic Resource
    Regulation of enzyme levels by phytochrome in mustard cotyledons: Multiple mechanisms? (1977)
    Springer
    Planta 136 (1977), S. 181-186 
    Details
    ISSN: 1432-2048
    Keywords: Enzyme Regulation ; Sinapis alba ; Phytochrome ; Phenylalanine ammonia-lyase ; Ribulosebisphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome controls the appearance of many enzymes in the mustard (Sinapis alba L.) cotyledons. The problem has been whether the effect of phytochrome on the appearance of enzymes in this organ is due to a common initial action of Pfr, e.g. due to the liberation of a “second messenger”. We have compared the modulation by light (phytochrome) of the appearance of phenylalanine ammonia lyase (PAL)+ and ribulosebisphosphate carboxylase (Carboxylase)+. PAL becomes detectable in the mustard cotyledons at 27 h after sowing while Carboxylase starts to appear only at 42 h after sowing (starting points, 25° C). The starting points cannot be shifted by light. As a major result, in the case of PAL the inductive effect of continuous red light (given from the time of sowing) remains fully reversible by 756 nm-light up to the starting point (27 h after sowing) while with Carboxylase full reversibility in continuous red light is lost at approximately 15 h after sowing. While the induction of Carboxylase is already saturated at a very low level of Pfr (e.g. continuous 756 nm-light saturates the response) and does not depend on irradiance (e.g. continuous 675 mW m-2 red light and 67.5 mW m-2 red light lead to the same time course), PAL induction is a graded response over a wide range of Pfr doses and depends strongly on the fluence rate (high irradiance response, HIR). It is concluded that PAL induction and Carboxylase induction are not only separated in time but differ in every regard except that both responses are mediated by phytochrome. The present data support the previous conclusion that the specification of the temporal and spatial pattern of development is independent of phytochrome even though the realization of the pattern of development can only occur in the presence of phytochrome (Pfr). It seems that there is no feedback from pattern realization to pattern specification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396196
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  • 9
    Electronic Resource
    Electronic Resource
    Phytochrome and potassium uptake by mung bean hypocotyl sections (1977)
    Springer
    Planta 137 (1977), S. 61-64 
    Details
    ISSN: 1432-2048
    Keywords: Phaseolus ; Phytochrome ; Potassium uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uptake of potassium (K) and 86rubidiumlabelled potassium (86Rb) by sub-hypocotyl hook sections of Phaseolus aureus L. was inhibited by red light. The effect was reversible with far red light. Using short exposures of high irradiance the effect on 86Rb-labelled K uptake was observed after 5 min. The response showed no specificity for a particular anion. Uptake of 86Rb-labelled K by sections cut immediately below the cotyledons was enhanced by red light after 10 min incubation and was also far red reversible. These results are interpreted as a rapid phytochrome-induced change in membrane properties resulting in modified K uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394436
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  • 10
    Electronic Resource
    Electronic Resource
    Phytochrome and calcium ions are involved in light-induced membrane depolarization in Nitella (1977)
    Springer
    Planta 137 (1977), S. 225-229 
    Details
    ISSN: 1432-2048
    Keywords: Calcium Ions ; Membrane potential ; Nitella ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated internodes of Nitella (N. opaca, N. flexilis) and Nitellopsis spec. were punctured with single microelectrodes and their membrane potentials were recorded continuously during various light treatments. In red light the initial response was always a depolarization. This depolarization began with a lag-time of 0.4-3.5s and reached a steady state within 1–2 min of continuous illumination. Repolarization began within several seconds after turning off the light. The magnitude of the red-light-induced depolarization increased with the Ca2+-concentration of the medium. The largest depolarizations were recorded in 5 m mol l-1 Ca2+. Ca2+ could not be replaced in this function by Na+, Mg2+, La3+ or mannitol. Far-red light alone had no effect on the resting membrane potential. Far-red light applied immediately after red light accelerated the repolarization of the membrane potential. Far-red light applied simultaneously with red light reduced the amount of depolarization and increased the rate of repolarization. The results indicate that phytochrome and Ca2+ are involved in the light-induced depolarization of the membrane. They are consistent with the hypothesis that phytochrome may act by triggering a Ca2+-influx at the plasma membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388154
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