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  • Springer  (61,770)
  • American Meteorological Society
  • 1995-1999  (63,117)
  • 1990-1994
  • 1997  (63,117)
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  • 1
    ISSN: 1420-9098
    Keywords: Key words: Mitochondrial DNA, restriction site polymorphism, meat ants, Iridomyrmex purpureus, polygyny.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: The Australian meat ant Iridomyrmex purpureus is known from observations and allozyme surveys to have a high proportion of mature colonies polygynous, but very few incipient ones. Sixteen colonies in the vicinity of Maryborough, Victoria, revealed four mtDNA restriction site haplotypes. One of these nests presented two different haplotypes; given the haplotype frequencies, this result suggests that a high proportion of mature nests contain unrelated queens.
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  • 2
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    Insectes sociaux 44 (1997), S. 15-21 
    ISSN: 1420-9098
    Keywords: Key words: Mangrove, ants, Polyrhachis sokolova, nest, inundation, foraging.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: The nest sites of the mud-nesting ant Polyrhachis sokolova were studied in Darwin Harbour mangroves. They were found from the Ceriops tagal zone to the Rhizophora stylosa zone at elevations ranging from 7.22 to 5.99 meters above the lowest astronomical tide (LAT), which means that the nests were inundated in 13‐61% of all high tides and for durations of up to 3.5 hours. The nest structure was studied by excavating nests and making a cast of the galleries using polyurethane foam. The nests were quite extensive, normally with two elevated nest entrances and galleries down to depths of 45 cm. The loose soil particles at the nest entrances collapsed when the tide reached them and formed a stopper which prevented water from intruding into the nest. In this way, the galleries remained dry during high tide. The ants showed a clear swimming or "walking on the surface" behaviour when they returned to the nest just before the entrance collapsed and during ebb. The tolerance of the ants to submergence was tested in the laboratory, with 50% mortality after 11 hours submergence in seawater at 23 °C, and only 3.5 hours in water at 33 °C. Therefore, the nesting behaviour with trapped air in the galleries is necessary for survival in these environments.
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  • 3
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    Insectes sociaux 44 (1997), S. 23-33 
    ISSN: 1420-9098
    Keywords: Key words: Diversity, chaparral, Formicidae, inventory methods, serpentine.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: In northern California, ant assemblages in 8 sites in serpentine chaparral habitat were compared with those occurring in 8 chaparral sites on adjacent non-serpentine soils. A total of 27 species of ants was found: 22 species were collected in non-serpentine chaparral and 20 species in serpentine chaparral. Seven species were collected exclusively in non-serpentine, and 5 species were found only in serpentine habitats. A Formica species, conspecific with or closely related to F. xerophila, was found only in serpentine chaparral. Subsequent collections suggest that in northern California, this species is confined to serpentine outcrops. Two other species significantly differed in frequency of occurrence in each habit:Camponotus hyatti, C. cf. vicinus. Five species showed marginal significant differences in their relative abundance between habitats:Camponotus hyatti, C. cf. vicinus, Formica moki, Prenolepis imparis, Leptothorax nevadensis, Stenamma diecki.
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  • 4
    ISSN: 1420-9098
    Keywords: Key words: Foraging activity, Meliponinae, Melipona favosa, M. fasciata, M. beecheii, Tetragonisca angustula.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: For Melipona fasciata, M. beecheii, M. favosa and Tetragonisca angustula we found distinct intergeneric differences in foraging activity patterns. The Melipona species had a longer daily foraging activity period than T. angustula. Pollen foraging patterns of the Melipona species were different from that of T. angustula: Melipona collect pollen earlier in the day. In an experimental habitat without competitors, Melipona favosa did not collect pollen very early in the day. ¶Individually marked returning foragers demonstrated in the course of the day distinct differences in nature and frequency of interactions with nest mates. Individual foraging flight frequency was lower and the intranidal transfer of nectar was delayed when syrup of a lower concentration was offered. ¶The regulation of daily foraging activity patterns and the nectar foraging behaviour of individual worker bees are discussed.
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  • 5
    ISSN: 1420-9098
    Keywords: Key words: Leptothorax, colony size, demography, polydomy, Formicidae.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: 484 nests of Leptothorax tuberointerruptus were collected from a coastal limestone grassland community in Dorset, England, over a 16 month period from February 1992 to May 1993. Behavioural observations and dissections of females strongly suggested that the nests were obligately monogynous. Electrophoretic data also suggested that within most nests all the workers shared the same mother and father. Counts of nest contents revealed temporary but dramatic drops in both worker and queen numbers per nest in May—June 1992. Seasonal polydomy is postulated to be the cause of this variation. Such dramatic variation highlights the importance of sampling over as much space and time as possible if premature conclusions about colony demography are to be avoided.
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  • 6
    ISSN: 1420-9098
    Keywords: Key words: Ants, foraging, interspecific competition, Myrmica sabuleti, Formica fusca.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary: Myrmica sabuleti forms mature colonies of about one thousand workers and a few queens. Although living in dry and sunny biotopes, this species avoids foraging during the warmer hours of the day which correspond to the period of the maximum foraging activity of two sympatric species, Formica fusca and F. cunicularia. M. sabuleti is the most frequently observed species on the extrafloral nectaries of Vicia sativa, even during the warmer hours of the day, whereas F. fusca is the most frequent species during those hours at tubes of 1M sucrose solutions placed on the ground. In spite of this temporal segregation, the foraging activity of M. sabuleti and F. fusca overlaps two times a day (10:00‐11:00 am and 5:00‐8:00 pm). Newly discovered large food sources are exploited by M. sabuleti through an explosively increasing recruitment, whereas workers of F. fusca forage mainly individually. Interference between M. sabuleti and F. fusca was studied when offering sucrose solutions, large dead insects unretrievable by individual foragers of either species (cockroaches), medium-sized insects retrievable by single foragers of F. fusca but not by single M. sabuleti(larvae of Calliphora erythrocephala) and small insects (Drosophila) retrievable by individual foragers of both species. Owing to its rapid recruitment, M. sabuleti is able to displace F. fusca from sucrose solution and large dead prey during the overlaping of the activity cycles of both species. When medium-sized corpses are offered, the issue depends on the speed of the events. F. fusca is able to take the prey away only if it discovers it before M. sabuleti has recruited nestmates. Small dead prey do not lead to interference, but is taken by the first worker that finds it.
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  • 7
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    Pure and applied geophysics 149 (1997), S. 299-312 
    ISSN: 1420-9136
    Keywords: Key words: Peak acceleration, moment magnitude, hypocentral distance, Kamchatka.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —To estimate for the first time the typical relation between peak acceleration A max , moment magnitude M W and hypocentral distance R for Kamchatka, 101 analog strong motion records for 1969–1993 were employed as the initial data set. Records of acceleration and velocity meters were obtained at 15 rock to medium-ground Kamchatkan sites from 33 earthquakes with M W = 4.5–7.8, at R = 30–250 km. A max values were determined from "true" acceleration time histories calculated by spectral deconvolution of digitized records. The maximum value over the two horizontal components was used as the A max value in the further analysis. With the scarce data available, there were no chances to determine reliably the whole A max (M W  , R) average surface; thus the shape of this trend surface was determined on a theoretical basis and only the level was fitted to the data. The theoretical model employed included: (1) source spectrum: according to the Brune's spectral model; (2) point-source attenuation: as 1/R plus loss specified by Q(f) = 250 f 0.8; (3) finite-source correction for a disc-shaped incoherent source, its size depending on M W  ; (4) accelerogram duration: including source-dependent and distance-dependent terms; (5) A max value: based on random process representation. Distance trends calculated with this model agree with the empirical ones of FUKUSHIMA and TANAKA (1990). To calculate the absolute level for these trends, observed A max (M W  , R) values were reduced to M W = 8, R = 100 km using the theoretical trends as reference. The median of the reduced values, A max (8, 100), equal to 188 gal. was taken as the absolute reference level for the relation we sought. Note that in the process of data analysis we were forced to entirely reject relatively abundant data of two particular stations because of their prominent local amplification (×5.5) or deamplification (×0.45).
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  • 8
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    Pure and applied geophysics 149 (1997), S. 525-540 
    ISSN: 1420-9136
    Keywords: Key words: Narmada-Son Lineament, lower crust, layering.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —Comparison of deep seismic sounding (DSS) results of different profiles across the Narmada-Son Lineament (NSL), India indicates the anomalous nature of the crust along the Ujjain-Ma han profile. Forward travel time and synthetic seismogram modeling, using normalized record sections of refraction and wide angle reflection data acquired along the Ujjain-Mahan deep seismic sounding profile across NSL, brings into focus the presence of high velocity (7.0–7.3 km/s) subhorizontal layers from a depth of 8–12 km down to Moho. The tectonic implication of such reflections (layering in the crust) is discussed. The two fault zones, reported by earlier workers, flanking the rift might have acted as feeders for the mantle material to intrude into the middle and lower crustal columns.
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  • 9
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    Pure and applied geophysics 149 (1997), S. 541-551 
    ISSN: 1420-9136
    Keywords: Key words: Synthetic seismograms, absorption and dispersion.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —An algorithm has been developed to compute the dispersive and dissipative seismic response using FUTTERMAN’S (1962) third attenuation-dispersion relationship. In the computation, frequency-dependent velocity and quality factor Q have been used but in the case of the nondispersive synthetic seismogram, frequency-independent velocity has been used. The model’s parameters are density, phase velocity, quality factors and thicknesses of the layers. Dispersive and nondispersive synthetic seismograms have been computed with and without absorption for a layered earth geological model. Fast Fourier transform (FFT) technique has been adopted for converting the frequency domain response into the time domain. The frequency spacing, Δf = 0.976 Hz, has been considered to avoid the aliasing effect. The results have revealed changes in the reflected waveforms in the frequency domain as well as in the time domain for absorption and dispersion cases. It is also concluded that dispersion reduces the arrival time and this effect is increasing with the travel time. The effect of constant Q on the seismic response has also been studied.
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  • 10
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    Pure and applied geophysics 149 (1997), S. 553-574 
    ISSN: 1420-9136
    Keywords: Key words: Correlation dimension, Hurst exponent, point process, seismicity, Weibull distribution.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —Deception Island is characterized by small magnitude local events with constant energy flux and very low stress drop. To obtain information about its origin, an interevent time series of 546 events, corresponding to an observational period of two month, has been analyzed. From a statistical point of view, data satisfies a Weibull distribution and presents clustering. A rescaled range analysis reveals that data are not independent, i.e. have memory, and the correlation dimension saturates at 2.2; as a consequence, the system can be modeled as a nonlinear iterative equation with three degrees of freedom that presents chaotic behavior. Taking into account that the average interevent time is of the order of 130 minutes, too short to be only due to tectonic activity, the above results indicate that some other mechanism may coexist with the regional tectonic one. According to several geological and geophysical observations, we suggest that most of the local events may be originated by pressure waves generated by a sudden change of phase, of sea and fresh water infiltrated into the main fractures and faults and also from shallow and confined water-saturated layers.
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  • 11
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    Pure and applied geophysics 149 (1997), S. 623-639 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
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  • 12
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    Pure and applied geophysics 149 (1997), S. 775-792 
    ISSN: 1420-9136
    Keywords: Key words: Quasi-biennial oscillation, atmospheric temperature.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —The 4-season (12-month) running means of temperatures at five atmospheric levels (surface, 850–300 mb, 300–100 mb, 100–50 mb, 100–30 mb) and seven climatic zones (60°N–90°N, 30°N–60°N, 10°N–30°N, 10°N–10°S, 10°S–30°S, 30°S–60°S, 60°S–90°S) showed QBO (Quasi-biennial Oscillation), QTO (Quasi-triennial Oscillation) and larger periodicities. For stratosphere and tropopause, the temperature variations near the equator and North Pole somewhat resembled the 50mb low latitude zonal winds, mainly due to prominent QBO. For troposphere and surface, the temperature variations, especially those near the equator, resemble those of eastern equatorial Pacific sea-surface temperatures, mainly due to prominent QTO. In general, the temperature trends in the last 35 years show stratospheric cooling and tropospheric warming. But the trends are not monotonic. For example, the surface trends were downward during 1960–70, upward during 1970–82, downward during 1982–85 and upward thereafter. Models of green-house warming should take these non-uniformities into account.
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  • 13
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    Pure and applied geophysics 149 (1997), S. 761-774 
    ISSN: 1420-9136
    Keywords: Key words: Triad interactions, cross-spectral technique, wave-CISK process.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —In order to understand the physical mechanism for maintenance of low frequency waves, computations of nonlinear kinetic energy (KE) exchanges into individual triad interactions in frequency domain by use of cross-spectral technique over global tropics (20°S–30°N) at 850 hPa and 200 hPa for June, July and August of 1988 are carried out. The KE exchanges among different frequencies and the interactions between the transient eddies and the time mean flow are found to be an order of magnitude smaller in the lower troposphere than those in the upper troposphere. The results show two distinct spectral peaks of periods 45 and 25 days in nonlinear energy transfer in the lower troposphere. The former is more pronounced than the latter. The role of time mean flow on the low frequency transients is found to be secondary compared to the effect of the leading term due to nonlinear interactions in the lower troposphere. Low frequency waves suffer a net loss of energy in the upper troposphere. In the lower troposphere, north of 20°N low frequency waves lose energy through nonlinear triad interactions, unlike the upper troposphere where gain of energy is noticed. Longitude-frequency distributions suggest that wave-CISK process and strong gradient of SST are the possible mechanisms for the strong energy interactions associated with low frequency waves in the lower troposphere over the west Pacific and east coast of Africa, respectively. The study may aid investigation of the rapid loss of predictability of low frequency modes over the tropics.
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  • 14
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    Pure and applied geophysics 149 (1997), S. 809-834 
    ISSN: 1420-9136
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  • 15
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    Pure and applied geophysics 149 (1997), S. 689-706 
    ISSN: 1420-9136
    Keywords: Keywords: Slip distribution, stress drop, strong ground motion, Hurst exponent, fractional Brownian motion, fractal dimension.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —The characteristics of slip and stress drop distributions accompanying earthquakes are explored from the perspective of fractional Brownian motion (fBm). Slip and stress drop distributions are assumed to be processes of fBm.The Hurst exponent (H), which reveals the roughness of a random process of fBm, is first estimated from ten inferred slip maps for six crustal earthquakes occurring in California. The relationships between the Hurst exponents with respect to static slip (H u ),stress (H τ ), static stress drop (H δσ ) and slip velocity (H ú ) are then established following Andrews (1980). They are found to be H δσ = H τ = H u -1 = H ú -0.5. Empirically, H u is recognized as being about 1 which, according to the theory of fBm, implies that the static slip distribution of an earthquake is just on the margin between being and not being self-similar, depending on the individual case. Cases where H u is less than 1 (i.e., self-similar) suggest that H δσ 〈 0(i.e., the distribution of static stress drop diverges), which is, in light of fBm, invalid. One possible explanation for this paradox is that H u is less than 1 in crustal earthquake phenomena only over a certain specific bandwidth of wavenumbers, or it could be that the relation H δσ = H u -1 is not valid, which implies that static stress drop in the wavenumber domain is not the product of stiffness and slip as described in Andrews (1980). It could be that some different physics apply over this particular bandwidth. In such cases, multi-fractals may be a better way to explore the characteristics of the Hurst exponents of slip. In general, static stress drop and stress distributions are more likely to be self-similar than static slip distribution. H u ≌ 1 and H δσ ≌ 0 are good first approxima tions for the slip and stress drop distributions. The spectrum of ground motion displacement falls off as ω -(Hδσ + 2) with H δσ ≌ 0, consistent with an ω-2 model of the earthquake source.
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  • 16
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    Pure and applied geophysics 150 (1997), S. 113-120 
    ISSN: 1420-9136
    Keywords: Key words:ω-values, circum-Pacific belt, mechanical structure.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —A data set of earthquakes which occurred in the circum-Pacific regions has been used to compute the ω values. This parameter is obtained through Gumbel’s third asymptotic distribution of extreme values and is well known as upper bound magnitude. The results show relatively higher ω values in South America, Central America and Mexico, than in the western part of the circum-Pacific belt. The results are interpreted in terms of the different mechanical structure (heterogeneity) of the materials in the eastern and western parts of the examined area.
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  • 17
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    Pure and applied geophysics 150 (1997), S. 37-52 
    ISSN: 1420-9136
    Keywords: Key words: Spectrum analysis, magma, Vp/Vs ratio.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —The Nikko region, in northern Kanto, central Japan, which is a high seismicity region, is located near the volcanic front. Many seismic observations have been carried out around the Nikko region because the seismograms of the shallow earthquakes occurring in this region show an S-wave reflected phase (SxS) and an S-to-P mode-converted and reflected phase (SxP) from a mid-crustal seismic velocity discontinuity. The depth of the mid-crustal reflector was estimated to be about 9–16 km. From the amplitude analyses of SxS and SxP phases, the material of the mid-crustal layer just beneath the reflector was expected to be a low rigidity layer, which was interpreted as partially molten rocks. No evidence has been shown for the internal properties of the material of the reflector. The Vp/Vs ratio is a good measure to distinguish between the two candidates for seismic reflectors; i.e., partially molten rocks and rocks containing water. We investigate the Vp/Vs ratio of the reflector using the spectrum analyses of the data observed by a seismic array. From spectrum analysis of the data, we indicate that the Vp/Vs ratio of 2.5 is better than that of 1.8 to explain the observed data, which shows that the material beneath the reflector consists of partially molten rocks.
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  • 18
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    Pure and applied geophysics 150 (1997), S. 1-18 
    ISSN: 1420-9136
    Keywords: Key words: Coseismic surface deformation, terrain effects, the finite element method.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —Coseismic surface deformation provides important information needed to determine source rupture geometry and slip distribution as well as to estimate seismic moment. In this study, numerical experiments were designed to analyze and classify how free-surface topography affects surface deformation. The investigation was performed by 3-D finite element modeling. Results of this study show that crustal deformation induced by near-fault terrain is significant and can be measured with present geodetic survey techniques. The characteristics of the terrain effects show that a hill structure produces more crustal deformation than a half-space model, and that the crustal deformation of a basin structure is less than that of the half-space model. The topographic correction is in the order of five percent of the fault dislocation. On the basis of the relationship between fault offset and earthquake magnitude, it is suggested that the terrain effects on the coseismic crustal deformation of shallow earthquakes with a magnitude greater than 5.6 should be considered as one of the major errors in coseismic deformation modeling which ignored the surface topography on the order of 300 meters.
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  • 19
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    Pure and applied geophysics 150 (1997), S. 157-180 
    ISSN: 1420-9136
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  • 20
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    Pure and applied geophysics 150 (1997), S. 285-304 
    ISSN: 1420-9136
    Keywords: Key words: Campi Flegrei, attenuation, predominant frequency, azimuthal variations.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —In this paper we have determined apparent Q from body-wave dispersion and a spectral ratio method for the Campi Flegrei area. A contour map of azimuthal variations of apparent Q (ranging from Q = 4 to Q = 40) reveals that the lower Q (higher attenuation) corresponds to an area that includes the Solfatara Crater (with active fumaroles), near the site of maximum uplift produced during the bradyseismic crisis of 1984, and just over the site evidencing the presence of a magmatic body. A strong azimuthal variation of the predominant frequency of the first pulse of the P wave has been found and is interpreted in terms of a vertical travel time in a surficial resonant layer (∼ 1 km thick) over a halfspace. The attenuation maps, the interpretation of the resonant frequencies, and the additional information provided by the P-wave velocity suggest the presence of a circular structure in the area. Not only does the distribution of the thickness of the resonant layer (obtained through the estimation of the contribution of different harmonics) show the circular structure, but it also agrees with the presence of a thin layer of lighter sediments, determined by means of a microgravimetric study, and with the total magnetic map, previously obtained for this area. The results of the azimuthal variation provide new evidence of the structure that can be used to improve the knowledge of the area.
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  • 21
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    Pure and applied geophysics 150 (1997), S. 329-340 
    ISSN: 1420-9136
    Keywords: Key words: Maximum entropy spectral analysis, high latitude indices, MILDCAAs, horizontal (H) component, vertical (Z) component.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —Maximum entropy spectral analysis (MESA) has been applied to 24 series of hourly daily data and only one daily mean series for the horizontal (H) and vertical (Z) components of the geomagnetic field for the year 1983 as observed at Narssarssuaq, Greenland (71.2°N, 36.7°E) (gm coordinate). The method has isolated some prominent medium frequency signal components. The maximum peaks for H are at 06 hr (0.174 cycles per day (cpd), 3.2 × 104 db) and 08 hr (0.09 cpd, 3.5 × 104 db). Similarly, the maximum peak in Z is observed at 04 hr (0.114 cpd, 5.7 × 104 db). The spectral results for the daily-mean data indicate periods are greater than two days, with 178.5 days (nearly semiannual) being common to both H and Z. Other harmonics have been found for all the series of H and Z components which are mainly caused by the "Effective Period", i.e., the period produced by the combined effect of the sunspot numbers and the sun’s rotation period. Such frequencies correspond very well with those found in the geomagnetic indices A p  , C p and AE. This suggests that the disturbance transient variations are caused by viscous interaction of the solar energies emanating from sunspot regions with the outermost magnetospheric boundary which, in turn, influences the magnetosphere-iono sphere coupling and produces the medium intensity long-duration continuous auroral activities (MILD CAAs) over high latitude regions. Thus, the higher latitude geomagnetic activities are nothing but the "effective period driven MILDCAAs" having a recurrence tendency of 27/n, where n is an integer.
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  • 22
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    Pure and applied geophysics 150 (1997), S. 217-248 
    ISSN: 1420-9136
    Keywords: Key words: Earthquake precursors, Loma Prieta earthquake, fault zones, crustal fluids, electromagnetic theory, crustal conductivity.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —The 1989 M s = 7.1 Loma Prieta earthquake was preceded for 12 days by what have been claimed as precursory ultra-low-frequency (ULF) magnetic noise anomalies ten times background, and by a very high peak up to 100 times background just 3 hours before the earthquake. We propose that these anomalous fields could have been due to the formation of a long thin highly-conductive region along the earthquake fault, which magnified the external electromagnetic waves incident on the earth’s surface. We use a simplified quantitative model, assuming a highly-conductive elliptic cylinder embedded in a layered resistivity structure, which we base on independent magnetotelluric measurements. The magnetic-field anomaly observed 3 hours before the main shock can be modeled by assuming an elliptic conductor extending from the surface to the hypocenter with a conductivity of 5 S · m−1. Our computed anomaly matches the observed anomaly to within a deviation of 35% over an observed frequency range of over 2 orders of magnitude, over which the measured anomaly varies from only about twice background (at 5 Hz) to about 100 times background (at 0.01 Hz). In addition, other anomalies recorded up to 12 days before the earthquake, can be modeled in detail by varying only the size of the elliptic conductor.¶We show that such an increase in conductivity could be caused by a precursory reorganization of the geometry of fluid-filled porosity in the fault-zone, which we call a dilatant-conductive effect. The extreme observed magnetic anomalies can be modeled using the high fault-zone porosity (c. 10%) and fluid conductivity (equivalent to 2 M NaCl) implied by other workers’ magneto-telluric measurements, but without requiring the large-scale precursory fluid flow characteristic of other published models for the magnetic-field precursors.
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  • 23
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    Pure and applied geophysics 150 (1997), S. 381-391 
    ISSN: 1420-9136
    Keywords: Key words: Wall-rock velocities, rockbursts, slab buckling.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract. —Considerable evidence has been reported for wall-rock velocities during rockbursts in deep gold mines that are substantially greater than ground velocities associated with the primary seismic events. Whereas varied evidence suggests that slip across a fault at the source of an event generates nearby particle velocities of, at most, several m/s, numerous observations, in nearby damaged tunnels, for instance, imply wall-rock velocities of the order of 10 m/s and greater. The common observation of slab buckling or breakouts in the sidewalls of damaged excavations suggests that slab flexure may be the mechanism for causing high rock ejection velocities. Following its formation, a sidewall slab buckles, causing the flexure to increase until the stress generated by flexure reaches the limit S that can be supported by the sidewall rock. I assume here that S is the uniaxial compressive strength. Once the flexural stress exceeds S, presumably due to the additional load imposed by a nearby seismic event, the slab fractures and unflexes violently. The peak wall-rock velocity v thereby generated is given by ¶¶ v = (3 + (1 - v²)/2)½ S/(ρE)½¶¶for rock of density ρ, Young's modulus E, and Poisson's ratio ν. Typical values of these rock properties for the deep gold mines of South Africa yield v = 26 m/s and for especially strong quartzites encountered in these same mines, v 〉 50 m/s. Even though this slab buckling process leads to remarkably high ejection velocities and violent damage in excavations, the energy released during this failure is only a tiny fraction of that released in the primary seismic event, typically of magnitude 2 or greater.
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  • 24
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    Pure and applied geophysics 150 (1997), S. 473-492 
    ISSN: 1420-9136
    Keywords: Key words: Mechanism of reservoir-induced seismicity, Koyna, Monticello Reservoir, Lake Mead.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract. —In most cases of reservoir-induced seismicity, seismicity follows the impoundment, large lake-level changes, or filling at a later time above the highest water level achieved until then. We classify this as initial seismicity. This "initial seismicity" is ascribable to the coupled poroelastic response of the reservoir to initial filling or water level changes. It is characterized by an increase in seismicity above preimpoundment levels, large event(s), general stabilization and (usually) a lack of seismicity beneath the deepest part of the reservoir, widespread seismicity on the periphery, migrating outwards in one or more directions. With time, there is a decrease in both the number and magnitudes of earthquakes, with the seismicity returning to preimpoundment levels. However, after several years some reservoirs continue to be active; whereas, there is no seismicity at others. Preliminary results of two-dimensional (similar to those by Roeloffs, 1988) calculations suggest that, this "protracted seismicity" depends on the frequency and amplitude of lake-level changes, reservoir dimensions and hydromechanical properties of the substratum. Strength changes show delays with respect to lake-level changes. Longer period water level changes (∼1 year) are more likely to cause deeper and larger earthquakes than short period water level changes. Earthquakes occur at reservoirs where the lake-level changes are comparable or a large fraction of the least depth of water. The seismicity is likely to be more widespread and deeper for a larger reservoir than for a smaller one. The induced seismicity is observed both beneath the deepest part of the reservoir and in the surrounding areas. The location of the seismicity is governed by the nature of faulting below and near the reservoir.
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  • 25
    ISSN: 1420-9136
    Keywords: Key words: Reservoir-induced seismicity, nucleation, pore pressure.
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    Topics: Geosciences , Physics
    Notes: Abstract. —Earthquakes continue to occur in the vicinity of Shivaji Sagar Lake since its creation by the Koyna Dam in 1962. The seismicity peaked in 1967 with a M 6.3 earthquake which claimed over 200 human lives and destroyed the Koyna township. Earthquakes of M≥ 4 occur every year following an increase of water level in the reservoir. During 1973, 1980 and 1993–94 earthquakes exceeding magnitude 5 occurred. Most earthquakes of M≥ 4 are associated with pronounced foreshocks and aftershocks. Starting Sepember 1993, seismic monitoring was vastly improved with the deployment of additional close-by stations (analog and digital). The focal parameters now available have enabled delineation of the active faults and deciphering of the earthquake nucleation process. During 1995–96, 13 boreholes were drilled to depths of 130 to 250 m and measurement of water levels in these wells was initiated. A preliminary analysis of one year's data from a borehole 1 km south of Koyna reveals tidal signatures, indicating connection of the well to a confined aquifer which is favorable for detection of pore pressure anomalies induced by crustal strain. We hope to improve our understanding of the genesis of reservoir-induced earthquakes at Koyna with these new measurements.
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    Pure and applied geophysics 150 (1997), S. 461-472 
    ISSN: 1420-9136
    Keywords: Key words: Reservoir-induced seismicity, mine-induced seismicity, collapse earthquakes, Liaoning Province.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract. —We describe three types of induced seismicity observed in Liaoning Province, China reservoir-induced seismicity, mine-induced seismicity and collapse earthquakes. A shock with magnitude M = 5.2 took place on December 22, 1974 at Shenwo Reservoir and some smaller earthquakes caused by impoundment also took place near other reservoirs. Numerous earthquakes associated with mining activity occurred in some coal mines. 56 collapse earthquakes with magnitude of M 〉 1.8 occurred at Binggou coal mine in Jianchang county. An analysis of the cause and some features of these three categories of the induced earthquakes are described in this paper.
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    Experimental biology online 1 (1997), S. 1-8 
    ISSN: 1430-3418
    Keywords: Allometry ; Scaling ; Muscle ; Work loops ; Power output
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fibre bundles or whole muscles from Xenopus laevis, ranging in size from 0.5-60g, were studied. Maximum power output of predominantly fast (sartorius) and slow (adductor magnus) muscles was measured at cycle frequencies between 0.5 and 20Hz, using the work loop technique. Power output was highly dependent on cycle frequency, and in 50-60g adults was maximal at 6 Hz for fast fibres (65 Wkg-1), and 2 Hz for slow fibres (14 Wkg-1). The cycle frequency for maximum power output was dependent on body mass (Mb), and decreased as a function of Mb-0.07 in fast fibres, and Mb-0.23 in slow fibres. The functional significance of these differences is discussed.
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  • 28
    ISSN: 1430-3418
    Keywords: Concanavalin A receptors ; Recognition ; Chemosensory behaviour ; Tetrahymena
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    Topics: Biology
    Notes: Abstract The relationship between concanavalin A (ConA) receptors and the chemosensory behaviour of the ciliated protozoan Tetrahymena thermophila was studied using the peptide chemoattractants proteose peptone and fibroblast growth factor. Studies on the chemosensory behaviour in semisolid methylcellulose showed that 50 μg/ml ConA selectively inhibited the persistent element of swimming behaviour by reducing time runs of cells responding to proteose peptone from 12.2±4.5 min to 0.8±0.3 min. Methyl-alpha-D-mannoside, but not methyl-alpha-D-galactoside, abolished the inhibitory effect of ConA, suggesting that mannoside-containing ConA receptors are involved in maintaining a persistent swimming behaviour. Control experiments, carried out in liquids where persistent swimming is less important for cellular behaviour, showed that ConA did not affect proteose-peptone-induced chemoattraction under these conditions as measured by a two-phase assay for chemoattraction. Also, no inhibitory effect of ConA could be found on swimming rates when individual velocities of ConA-treated cells were determined. When tested in liquid chemoattraction assays, ConA was found to be a weak but significant chemoattractant. Studies of the cellular location of ConA receptors on the plasma membrane of starved cells showed an unequal distribution. A preferential clustering of receptors at the anterior end of the cell was observed when determined at high concentrations (100 μg/ml) of fluorescent ConA. Methyl-alpha-D-mannoside but not methyl-alpha-D-galactoside abolished the fluorescent ConA labelling, indicating a preferential clustering of these mannoside-containing receptors at the anterior part of the plasma membrane and cilia. At lower concentrations (25 μg/ml), FITC-ConA produced more general labelling of the entire cell membrane. The results suggest that ConA receptors are necessary for the persistent element of swimming and that binding of ConA to its receptors interferes with processes related to signal transduction rather than by limiting the free movement of cilia required for locomotion. The gradient of receptors seen at high FITC-ConA concentrations may be important for a putative spatial chemosensory mechanism, i.e. chemotaxis.
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    Experimental biology online 1 (1997), S. 1-11 
    ISSN: 1430-3418
    Keywords: Vocalization ; Respiration ; Squirrel monkey ; Lung-pressure modulation
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    Topics: Biology
    Notes: Abstract Respiratory abdominal movements during vocalization were measured in awake squirrel monkeys during spontaneous and playback-induced vocal activity. Large vocalization-correlated respiratory movements (VCRM) starting before vocalization were observed during several call types, such as peeping, trilling, cackling and err-chuck. Purring, in contrast, was accompanied by only small VCRM that started late after vocal onset. VCRM during trilling, a call with marked frequency modulation, showed a modulation in the rhythm of the frequency changes. A correlation with amplitude modulation was also present but more variable. As high frequencies need a higher lung pressure for production than low frequencies, the modulation of VCRM seems to serve to optimize the lung pressure in relation to the vocalization frequency. The modulation, furthermore, may act as a mechanism to produce different trill variants. During err-chucks and staccato peeps, which show a large amplitude modulation, a non-modulated VCRM occurred. This indicates the existence of a laryngeal amplitude-controlling mechanism that is independent of respiration.
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    Experimental biology online 1 (1997), S. 1-10 
    ISSN: 1430-3418
    Keywords: Leech skin ; Tight epithelium ; Hirudo medicinalis ; Pesticides ; Heavy metals ; Pollutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of the present study was to investigate the effects of environmental pollutants, such as heavy metals and pesticides on ion transport across the skin of the leech (Hirudo medicinalis). We wanted to examine the suitability of this epithelium as a model system for studies concerning the mechanisms of toxic action caused by environmental pollutants. For this purpose we performed Ussing chamber experiments to test three representative heavy metals and pesticides, respectively, for their effects on current flow across leech dorsal integument. Two representatives of each substance class showed distinct effects on ion transport across this epithelium. The heavy metal ions Pb2+ and Hg2+ produced a significant inhibition of amiloride-sensitive Na+ transport across leech skin in concentrations below or close to their limiting values in waste water. Therefore, it seems feasible to use leech skin for future investigations of the toxic actions of these heavy metals. The fact that Pb2+ and Hg2+ exerted their effects only when applied apically points to a specific action of these divalent cations on ion channels in the apical membrane. However, this inhibition does not seem to be a general feature of divalent cations because Cd2+ did not influence ion transport across leech skin at all. Since current flow through amiloride-sensitive Na+ channels in typical vertebrate tight epithelia is stimulated by numerous divalent cations, the pronounced inhibition of amiloride-sensitive Na+ channels in leech skin by Pb2+ and Hg2+ might lead to a further differentiation of amiloride-sensitive Na+ channels. The two widespread pesticides lindane and promecarb exerted their effects only at comparativ high concentrations. This low sensitivity restricts the usefulness of leech skin as a subject for further analysis of toxicity mechanisms, at least for these two pesticides.
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    Experimental biology online 2 (1997), S. 1-10 
    ISSN: 1430-3418
    Keywords: Dextran clearance ; Freshwater bivalve ; Inulin clearance ; PEG clearance ; Renal clearance ; Salinity tolerance ; Zebra mussel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Clearance of polyethylene glycol (PEG), inulin, or dextran that had been injected into the hemolymph of the mussel, Dreissena polymorpha, was measured in animals acclimated to pondwater (PW) or 10% seawater (SW). In addition, we measured the clearance of PEG from mussels acutely transferred into 10% SW and following return to PW after acclimation to 10% SW. Clearance values calculated for PW-acclimated mussels ranged from 2.0 to 3.3 ml (g dry tissue ċ h)-1 and declined to 0.28 ml (g dry tissue ċ h)-1 in 10% SW-acclimated animals. Transferring mussels into 10% SW resulted in a reduction in PEG clearance from the blood, coincident with the reduction of osmotic gradient. When 10% SW-acclimated mussels were returned to PW the clearance of PEG increased to rates observed in PW-acclimated animals within 1 h. The PEG clearance remained constant during the re-acclimation to PW even though the osmotic gradient declined from about 100 to 30 mosmol kg-1. Clearance of the solutes used in this study was likely to be a measurement of renal filtration rate. The clearance values appeared to be maximal when the animals were in PW. The limited capacity to increase clearance in the face of an osmotic challenge may be a critical factor in restricting D. polymorpha to freshwater or lower salinity environments with small ranges in salinity.
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    Experimental biology online 2 (1997), S. 1-4 
    ISSN: 1430-3418
    Keywords: Frankia ; Tetrazolium red ; Vital staining
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    Topics: Biology
    Notes: Abstract An improved method for determining the viability of the nitrogen-fixing actinomycete Frankia is presented. This method uses tetrazolium red as a vital stain, which proved more effective than a previously used method of acridine orange staining.
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    Experimental biology online 2 (1997), S. 1-12 
    ISSN: 1430-3418
    Keywords: Coelomic cells ; Eleocytes ; Nereis ; Nucleotides ; Polychaetes ; Sexual maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eleocytes (a type of coelomic cell) of the polychaete Nereis virens can store large amounts of adenine nucleotides at certain times. Since eleocytes have specific functions related to gametogenesis, we tested whether the presence of these large nucleotide stores in eleocytes is specific to gender or related to specific events during gametogenesis. Nucleotide pools in eleocytes isolated at different stages of sexual maturation from N. virens were analysed using high-performance liquid chromatography. Eleocytes from immature and male animals had extremely high concentrations of both AMP and ADP (each 〉 10 μmol/ml of packed cell volume). In eleocytes from male animals, the high nucleotide stores were maintained throughout the maturation phase and decreased at a late stage, while in female animals the nucleotides were degraded at an early stage of maturation. In male eleocytes, the decrease in the adenine nucleotide pool may be the result of its conversion to inosine which is then released by the eleocytes and reutilized by male germ cells for nucleic acid biosynthesis, as has been suggested previously. Our study shows that the time of degradation of the adenine nucleotide pool coincides with the period of spermatogonia proliferation which involves intense nucleic acid synthesis. ATP levels (0.4–1.5 μmol/ml packed cell volume) and the guanine nucleotide pool (GTP+GDP+GMP; 0.08–0.18 μmol/ml packed cell volume) were similar in both sexes, did not change during germ cell development and were decreased only in eleocytes from prespawning females. The GTP/GDP ratios were initially higher (up to 14) in eleocytes from females compared to ratios in eleocytes from immature (4–9) and male animals (up to 8), and decreased during the maturation phase of the animals. GTP levels were correlated with those of ATP; this correlation was much closer in eleocytes from females than from males. The results further support the hypothesis that the adenine nucleotide stores in the eleocytes are maintained as a supply of purine precursors for the growing germ cells.
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  • 34
    ISSN: 1430-3418
    Keywords: Macintosh simulation ; Rattlesnakes ; Searching behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Searchers in nature often have accurate knowledge of the spatial location of the resource targets they seek, though in many other cases they have none. For example, the spatial distribution of targets such as food patches or potential mates may shift or change unpredictably from season to season. Searchers encountering circumstances of these sorts may be said to be “naive”. This problem is compounded by the fact that spatial distributions of targets may vary statistically as well: they may be distributed randomly, uniformly, or they may be clustered. Accordingly, since we study an animal system in nature that encounters such challenges (i.e., free-ranging rattlesnakes in many parts of their range), we wrote a comprehensive spatial searching program for Macintosh systems that simulates this problem thoroughly, RattleSnake©. In a large series of experimental simulations using this software, we found that search paths of high vector magnitude (approaching 1.0), or those that approached straight lines, generated large numbers of collisions in large, clustered worlds. No search path was any better than any other in large, randomly or uniformly distributed worlds. Zig-zag paths of low vector magnitude (approaching zero) in small worlds of all types and of all densities were efficacious, due to continuous turning which prevented searchers from moving out of or exiting patches. Thus it appears that there are design rules in nature governing target collision probabilities in some but not all two-dimensional spatial worlds. Search paths of high vector magnitude, or those approaching straight lines, generate high collision frequencies in statistically clustered spatial worlds, for example. RattleSnake© thus may be useful in programs of basic and/or applied behavioral ecology, including conservation, as well as in laboratory and multimedia classroom education.
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  • 35
    ISSN: 1430-3418
    Keywords: Acherontia styx ; Azadirachtin ; Biogenic amines ; Development ; Nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous studies have suggested the involvement of biogenic amines in insect metamorphic events and post-embryonic development. The effect of azadirachtin (AZ), a natural antifeedant and growth-disrupting compound, on the nutrition, development, and biogenic amine contents of the last instar larvae of the Eastern Death’s Head hawk moth, Acherontia styx, was examined. Single doses of AZ, injected into the haemolymph at day 1 post-ecdysis, inhibited food consumption in a dose-dependent manner (ED50 = 0.65±0.08 μg AZ/g body weight), and was found to be highly effective at producing pupal deformities and inhibiting larval growth (0.1–0.2 μg AZ/g body weight range). Biogenic amine contents, namely octopamine (OA), dopamine (DA) and serotonin (5-HT), in the brain and the haemolymph of 4-day- and 8 day-old larvae were analysed using high-performance liquid chromatography (HPLC) with an electrochemical detector (ECD). A dose-response relationship between AZ and biogenic amine contents in the brain and the haemolymph was also established. Low doses of AZ (0.1–0.2 μg AZ/g body weight) caused a dramatic reduction in OA and 5-HT levels in both the brain and the haemolymph. However, higher doses (0.9–1.2 μg AZ/g body weight) were needed to induce a significant reduction in DA levels. The significance of these findings in relation to the possibility of the involvement of biogenic amines in regulating metamorphic events in insects through mediation of juvenile hormone synthesis and/or release is discussed.
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    Experimental biology online 2 (1997), S. 1-9 
    ISSN: 1430-3418
    Keywords: Algae ; Cryptomonad ; Diatom ; Dreissena polymorpha ; Fucus ; Gametes ; Spawning ; Zebra mussel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In zebra mussels (Dreissena polymorpha) simultaneous release of gametes and peaks in larval densities at particular locations suggest that spawning is triggered by synchronizing stimuli. Furthermore, spawning tends to occur only after an adequate environmental temperature is reached. To test the hypothesis that phytoplankton and gamete-associated chemicals initiate spawning in zebra mussels and that the responsiveness to such chemicals is affected by ambient temperature, the spawning response of zebra mussels to extracts from algae, a cryptomonad, and a cyanobacterium and to water associated with released gametes was assayed in animals acclimated to 12 ^C and 17 ^C. For animals held at 12 ^C, only serotonin, a known activator of bivalve spawning used as a positive control, stimulated spawning. However, for animals acclimated to 17 ^C, extracts made from a diatom (Phaeodactylum), a brown alga (Fucus), and a cryptomonad (Rhodomonas) stimulated spawning in both sexes; extracts from green algae (Platymonas and Dunaliella) and a cyanobacterium (Oscillatoria) did not cause spawning. Water associated with either released sperm or eggs elicited spawning in both females and males. Positive controls, stimulated with serotonin, spawned at a high (〉90%) rate, whereas no negative control spawned. Thus, phytoplankton chemicals and gamete-associated factors may have a role in synchronizing spawning in zebra mussels once adequate ambient temperature is reached.
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    Experimental biology online 2 (1997), S. 1-13 
    ISSN: 1430-3418
    Keywords: Spectrophotometer, fast scan ; light fiber, application ; absorption of turbid materials ; spectrophotometer, software ; absorption measurement, reflection measurement, luminescence measurement, fluorescence measurement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new optical spectrophotometer has been developed, based on a recently patented monochromator for spectral measurements of clear and, in particular, of turbid materials in the millisecond time range. The number of optical and mechanical components of the spectrophotometer has been reduced to a minimum, resulting in excellent light throughput, a low stray-light level, low cost, compactness and rigidity. The spectrophotometer has been designed for all kinds of spectral measurements such as absorption, transmission, reflection and luminescence/radiation in a single-beam mode as documented by several examples. In principle, there is no restriction of wavelengths, ranging from UV to NIR and up to the IR range. As many functions as possible are relocated from the hardware to the software part of the design, which allows for extraordinary flexibility and simplicity. An appropriate computer program providing data acquisition, control and calibration functions as well as real-time display of spectra has been developed on the basis of a compiler language; indispensable “fast routines” are written in assembler language.
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  • 38
    ISSN: 1430-3418
    Keywords: Cell deformation ; Monocyte ; Cytoskeleton ; Mechanical properties
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    Topics: Biology
    Notes: Abstract Blood leukocytes can exhibit extensive morphological changes during their passage through small capillary vessels. The human monocytic THP-1 cell line was used to explore the metabolic dependence of these changes in shape. Cells were aspirated into micropipettes for determination of the rate of protrusion formation. They were then released and the kinetics of morphological recovery was studied. Results were consistent with Evans’ model (Blood 64:1028, 1984) of a viscous liquid droplet surrounded by a tensile membrane. The estimated values of cytoplasmic viscosity and membrane tension were 162 Pa.s and 0.0142 mN/m respectively. The influence of metabolic inhibitors on cell mechanical behavior was then studied: results strongly suggested that deformation involved two sequential phases. The cell elongation rate measured during the first 30 s following the onset of aspiration was unaffected by azide, an inhibitor of energy production, and it was about doubled by cytochalasin D, a microfilament inhibitor, and colchicine, a microtubule inhibitor. However, during the following 2 min, deformation was almost abolished in cells treated with azide and cytochalasin D, whereas the protrusion of control cells exhibited an approximately threefold increase in length. It is concluded that, although cells seemed to deform as passive objects, active metabolic processes were required to allow extensive morphological changes triggered by external forces.
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  • 39
    ISSN: 1430-3418
    Keywords: Neutrophils ; Monocyte ; Actin ; Locomotion ; Adhesion ; Glucocorticoids
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    Topics: Biology
    Notes: Abstract A monocyte-derived steroid-induced factor has been shown previously to induce dispersive locomotion in human neutrophils and to lower adhesion to an albumin-coated glass surface. In this paper we show that this factor inhibits adhesion of neutrophils to bovine aorta and human endothelial cells by an undetermined mechanism. It induces unique changes in neutrophil shape with a characteristic monopolar pattern of F-actin distribution, which may correlate with the dispersive locomotion observed in the absence of a concentration gradient. This factor also inhibits N-formyl-methionyl-leucyl-phenylalanine-induced chemotaxis of neutrophils in a modified Boyden chamber assay. The reduction of adhesion and the inhibition of chemotaxis by the factor in vitro indicate a possible in vivo anti-inflammatory role.
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    The chemical educator 2 (1997), S. 1-2 
    ISSN: 1430-4171
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    Topics: Chemistry and Pharmacology
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    The chemical educator 2 (1997), S. 1-7 
    ISSN: 1430-4171
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    Topics: Chemistry and Pharmacology
    Notes: Abstract A mnemonic for the entire periodic table of the elements recalls the names and abbreviations of all the elements, their basic electron structures, and some properties. Elements are presented in chemically useful groupings with a method that allows one to position an element without recreating the entire table. With two additional lines one can derive all the major elements and the trace elements required by humans. Another verse identifies anomalies in orbital filling. The mnemonic and explanations can be fit on one double-sided page to provide a one-page reference.
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  • 43
    ISSN: 1430-4171
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Nuclear magnetic resonance spectroscopy holds a premier position as a tool for structure elucidation in organic chemistry. With the increased availability of high-field Fourier-transform spectrometers in undergraduate laboratories, there is an increased need for good instructional experiments. We describe a reliable one-step synthesis of a moderately complex structure, and a straightforward 1H NMR spectral assignment problem that illustrates the use of coupling constants for the determination of positional relationships, geminal coupling, and correlation spectroscopy (COSY) for the identification of coupled signals [1].
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    The chemical educator 2 (1997), S. 1-2 
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    The chemical educator 2 (1997), S. 1-1 
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    The chemical educator 1 (1997), S. 1-22 
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    The chemical educator 1 (1997), S. 1-10 
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    The chemical educator 1 (1997), S. 1-12 
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    The chemical educator 2 (1997), S. 1-1 
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    The chemical educator 2 (1997), S. 1-14 
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    The chemical educator 2 (1997), S. 1-10 
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    Notes: An essay assignment suitable for large introductory organic chemistry courses is described. Students were asked to write four-to five-page essays about any organic compound of their choosing that was in the news recently, was used widely, or had some social significance. The assignment was designed to stimulate student interest in the role of organic chemistry in their lives, to help them make connections between classroom information and the “real world”, to have them practice their writing skills, to teach them to use the library and other sources of scientific information, and to help them improve their grades by evaluating them with an instrument other than an exam. Techniques used by the instructor to manage the burden of grading a large number of essays are described.
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    The chemical educator 2 (1997), S. 1-11 
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    Topics: Chemistry and Pharmacology
    Notes: At California State University Fullerton the Departments of Chemistry and Biochemistry and Physics have jointly established an active learning instructional computer facility where students explore models and data in the upper division chemistry and physics curricula. This facility is also a component of the larger W.M. Keck Foundation Center for Molecular Structure (CMolS), a core research and education center where faculty and students throughout the California State University system have the opportunity for joint research and teaching activities directed at the determination and critical analysis of molecular structures. An array of Silicon Graphics workstations and a server housed in an “electronic classroom” provides a networking medium linking students and faculty across our curricula to resources and courses with common themes, but traditionally segregated. Through team teaching and utilization of resources and expertise across subdisciplines and disciplines, we are creating a learning pathway that coherently exposes our students in chemistry and biochemistry to more sophisticated problems and exploration. Computers provide visual reinforcement and inerpretation for concepts and principles that students may have difficulty understanding and that cannot be treated easily or well by the problem-solving methodology. Our students have responded enthusiastically to the electronic classroom, and introduction of chemical computation into the curriculum has had a positive pedagogical impact.
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    The chemical educator 2 (1997), S. 1-3 
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    Notes: Abstract A lecture demonstration of primary kinetic isotope effect using the bromination of acetone and acetone-d6 is described.
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    Notes: An undergraduate laboratory exercise to be used in an undergraduate instrumental methods course is presented for the electrochemical characterization of oxidation products of adrenaline. An objective of this experiment is to use information obtained through cyclic voltammetry and simulation software to develop a qualitative understanding of the oxidation mechanism of adrenaline.
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    The chemical educator 2 (1997), S. 1-7 
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    Notes: Abstract Although its origins arise from confidential information, this article emerges from an area gray enough - important enough - to make the author (hereafter: I) comfortable with the context and sufficiently cautious to clear the content with the National Science Foundation. Intrigued? Let me explain. My premise is that the way that grant proposals are written is representative of the way that a group of potentially leading-edge scholars
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    The chemical educator 2 (1997), S. 1-3 
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    Notes: Abstract The close of ChemConf’97 at the end of July gives me the opportunity to reflect on the effect of the Internet on scholarship and education in chemistry. This conference was first held in 1993, continued in 1996 and 1997, and is slated to run again September through December of 1997 and February through May of 1998. The quality of the papers presented and discussed at the last session was outstanding. The presenters included many of the premier names in chemical education. The Chemical Educator is proud to serve as an archive for one of those papers. This issue offers as its “sample article,” available free to anyone who registers at our Internet site, a paper presented at ChemConf’97 by Mary L. Swift and Theresa Zielinski. This paper “What Every Chemist Should Know About Computers II” addresses the challenges and opportunities associated with the use of computer technology in chemical education. We are also proud to have Hugh Cartwright, who presented his paper “Nature Doesn’t Solve Equations, So Why Should We? Mathematically-Lean Simulations in Chemistry” at the conference, on our Board of Editors and acting as our Media-Review Editor.
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    The chemical educator 2 (1997), S. 1-4 
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    The chemical educator 2 (1997), S. 1-7 
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    Notes: Abstract Solid phase microextraction (SPME) was developed to address the need for a fast, solvent-free and field compatible sample preparation method. It has been applied to a range of applications including environmental, industrial hygiene, process monitoring, clinical, forensic, food and drug analysis. In SPME, coated fibers are used to isolate and concentrate analytes into a range of coating materials. After extraction, the fibers are transferred, with the help of the syringe-like handling device, to analytical instruments for separation and quantification of the target analytes.
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    The chemical educator 2 (1997), S. 1-9 
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    The chemical educator 2 (1997), S. 1-12 
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    Notes: Abstract The results from a classic experiment in the undergraduate physical chemistry laboratory, the particle-in-a-box model for spectroscopic transitions of conjugated dyes, is compared to computational results obtained using a molecular mechanics structural approach and the extended Hückel molecular orbital picture. The goal of this exercise is to help students to think critically about their experimental data and to use comparisons of mathematical and computational models to try to understand departures of an experiment from expectations.
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    Notes: Abstract The acid-catalyzed hydrogen-deuterium exchange reaction in aliphatic ketones was modeled as a purely random process. Using a computer program, a statistically random hydrogen-deuterium exchange was simulated for a particular molecule, 2-indanone. For this molecule, there are two α carbons, each providing two potential sites for exchange. The program determined the relative amounts for each of the partially deuterated species, including the total percent deuteration. A comparison with mass spectral data showed good agreement with this model.
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    Notes: Abstract We present here a complete photochemical experiment suitable for biochemistry, bioorganic, and organic chemistry laboratories. It provides experiences in chemical and enzymatic syntheses, spectroscopy (IR, NMR, UV), chromatography (TLC, GC-MS), and a simple enzyme kinetic study utilizing UV spectroscopy. The application of light energy to produce chemical changes has recently expanded beyond photography, lithography, and organic synthesis to include use in tumor phototherapy [1] and as antiviral agents [2]. Dyes and a variety of photoactive chemicals have demonstrated potential use as environmentally benign insecticides, herbicides, and fungicides [3, 4]. Nevertheless, the traditional undergraduate chemical laboratory curriculum provides little exposure to photochemistry.
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    Notes: Abstract Goldschmidt combined a number of widely separated sciences to synthesize a new structural crystal chemistry. Although his work on the relative abundances of the elements, atomic and ionic radii, interionic distances, the effect of radius ratio on coordination number in crystals, replacement of ions in minerals, and the lanthanide contraction is found in almost every textbook of general and inorganic chemistry and has provided the basis for modern crystal chemistry and the use of size relationships for interpreting properties of inorganic substances, Goldschmidt’s name, life, and career remain relatively unknown to most chemical educators and practicing chemists. Goldschmidt used the basic properties of matter to provide simple and elegant explanations for the composition of our environment. Throughout his relatively brief career, filled with sorrow and tragedy, he continued to maintain his intense interest in the elements and their genesis, affinities, and associations despite his changes from one method to another in his attempts to obtain new and more complete data. Thus, although he used petrology, crystallography, and chemistry and enriched all these fields greatly, to him they were only tools for exploring the earth and its history.
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    The chemical educator 2 (1997), S. 1-8 
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    Notes: Abstract I use a simple rubber-tubing model to demonstrate principles of DNA topology, including DNA twist and supercoil, conservation of linking number, and the action of topoisomerases. The resilience of the rubber mimics the natural response of double-stranded DNA to distorting forces such as strand separation during replication. Teachers can use this demonstration in traditional lecture or adapt it as a group-learning exercise.
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    The chemical educator 2 (1997), S. 1-17 
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    Notes: Abstract A World Wide Web (WWW) site was developed on the subject of acids and bases for use as a supplement to the General Chemistry course. The site was used in two consecutive years of the course. Its effectiveness was assessed with surveys and with comparisons of grades for users and nonusers. Results show that students in general do not use the site to target their self-reported problem areas; instead, they tend to progress through the site in a linear fashion. Those students who used the site to help them with their problem areas were able to achieve a modest grade improvement over their performance on other exams.
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    The chemical educator 2 (1997), S. 1-21 
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    The chemical educator 2 (1997), S. 1-6 
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    The chemical educator 2 (1997), S. 1-10 
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Circular dichroism (CD) spectropolarimetry is one of only three analytical detector devices (sensors) capable of recognizing enantiomeric forms of optically active (chiral) molecules. The majority of naturally occurring substances that see significant applications as drug molecules are optically active. An intense interest in preparing chiral forms of drugs as single enantiomers has emerged with the rapid developments that are being made in the biotechnology industry. When a chiral drug is synthesized in the laboratory without the specificity normally provided in nature by enzymatically controlled reactions, the products are equimolar mixtures of both enantiomeric forms (racemicmixtures).
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  • 69
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    Notes: Abstract A convenient inorganic laboratory exercise is presented that exposes the student to the influences of ligand substituents on a metal center’s redox properties. Students prepare a series of compounds with the general formula [Ru(bpy)2(R1,R2mal)](PF6), where bpy is 2,2′-bipyridine and R1,R2mal is an anionic β-diketone ligand with various R1 and R2 substituents. Each complex is prepared in a single synthetic step, and the Ru(III)/Ru(II) couple is characterized by cyclic voltammetry. The potential of the Ru(III)/Ru(II) couple shifts upon varying R1 and R2 from 0.64 to 1.10 volts vs. SSCE in the following order: (R1 = R2 = Me) 〈 (R1 = R2 = Ph) 〈 (R1 = Me, R2 = CF3) 〈 (R1 = R2 = CF3). The inductive effects of the R substituents on the Ru(III)/Ru(II) couple are consistent with their electron-donating and electron-withdrawing properties. Hammett constants for the substituents provide an approximate measure of these effects. A linear relationship is observed when the Hammett constants are plotted against the Ru(III)/Ru(II) potential. This laboratory exercise applies a versatile electroanalytical method, cyclic voltammetry, to measure the substituent effects on a metal center’s electron density. Student results of the syntheses and redox characterizations of a series of [Ru(bpy)2(R1,R2mal)]+ complexes are presented and discussed.
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    Notes: Abstract A 10-week, open-ended experiment for a junior/senior-level integrated laboratory course is described. The project involves the synthesis and instrumental characterization of two monosubstituted and two disubstituted anthrylpolyamines of varying lengths, as well as a detailed investigation of their photophysical and photochemical properties in the presence of polyanions of biological interest. Depending on the nature of the polyanion, emission quenching of the anthracene chromophore occurs by a template-directed excimer formation, or by an energy-transfer process. A correlation between the charge of the protonated anthrylpolyamines and the degree of emission quenching is also investigated. This project is ideally suited for introducing students to different quenching mechanisms within the context of a research-oriented, integrated laboratory experience.
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    The chemical educator 2 (1997), S. 1-13 
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    Notes: Abstract In this paper, we describe the evolution of a laboratory syllabus for a course in Quantitative Analysis. Over the past two decades, the syllabus has changed from one having individual students do mostly ‘wet’ chemical analyses on commercial unknowns to one having groups of students do instrumental analyses on ‘real’ samples. We describe, in some detail, the current laboratory syllabus, which requires students to calibrate volumetric equipment, to determine the active ingredients in Dristan using UV-visible absorption spectroscopy, to determine calcium levels in over-the-counter tablets using both atomic absorption spectroscopy and an ion-selective electrode, to determine lead in wine bottle caps using differential pulse polarography, to measure pKa values for sulfonated naphthols using UV-visible absorption spectroscopy and to determine caffeine and aspartame in common beverages using HPLC. Group work is emphasized, and written reports are required. Students have responded quite positively to the current syllabus, especially to the use of ‘real’ samples.
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    The chemical educator 2 (1997), S. 1-17 
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    Notes: Abstract This paper probes the relationship between the psychometric construct known as ‘spatial ability’ and students’ performance in introductory chemistry courses. It examines some of the early literature on the evolution of the concept of spatial ability, reviews the results of research on the relationship between success (or failure) in introductory chemistry courses and students’ spatial ability, and describes a spatial ability test known as The Purdue Visualization of Rotations (ROT) test that has been shown to be among the spatial ability tests whose results are least likely to be complicated by analytical processing.
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    The chemical educator 2 (1997), S. 1-2 
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    Notes: Abstract The collective memory of the work of undergraduate student clubs is often fleeting, perhaps even more so than the memory of some academic faculty committees! Unlike fraternal organizations, with which members still identify long after they leave their student years, undergraduate students in preprofessional clubs more naturally dis-identify with their organizations once they
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    Notes: Abstract Going from almost zero to more than sixty active members in less than a year, the University of Michigan American Chemical Society Student Affiliate chapter is a success story about revitalizing a struggling chapter. Innovative officer positions, increased delegation of authority, and courage to try new ideas generated this special chemistry. In order to encourage each member to be creative and reach his or her full chapter potential, the officers stressed, ‘Dream of an outstanding activity, and everyone will make it happen.’ Strategically, this philosophy was reinforced by electing members who had spearheaded projects to the new officer positions. In the following article, we will present the events and philosophies that created the strong intra-Student Affiliate and inter-Student Affiliate/community chemical bonds.
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    The chemical educator 2 (1997), S. 1-4 
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    The chemical educator 2 (1997), S. 1-3 
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    Notes: Abstract I recently attended the national American Chemical Society meeting in Las Vegas (September 1997) and previous to that in San Francisco (April 1997). While there, I visited the Student Affiliates poster sessions that were held at those meetings. I did this both out of my own interest in undergraduate research (I have had five students present posters at national ACS meetings.) and to encourage the ACS Student Affiliates presenting successful chemistry club activities to contribute to this journal.1 I had a student presenter at one of the first of these events, San Francisco in April of 1992, and the growth of the student participation has amazed me. The sessions are now split into the subdisciplines of chemistry and there are more presentations at each of these than there were altogether in 1992. This makes me wonder what has sparked this growth in undergraduate research.
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    Notes: Abstract An attenuated total internal reflectance (ATR) cell has been designed and constructed by a group of four undergraduate and graduate students during an advanced laboratory course in the School of Chemical Engineering at Purdue University. Details for the assembly, which utilizes commercially available optical components, are given in this paper. The cell employs a 45-degree trapezoidal ZnSe crystal as the ATR element. Both spherical and flat gold-coated mirrors are used to focus and align the IR beam. The cell design presented here not only provides practical instrumentation design and implementation experience for students, it also has four major advantages imporatant for teaching purposes: a) It can be ported between different FTIR spectrometers with similar sample-compartment sizes; b) it provides an economical means for ATR spectroscopy in laboratory courses as the cost of this cell is less than half the price of similar commercial cells; c) all optical parts of the cell are easily accessible and visible for demonstration and adjustment purposes; and d) it can serve as a starting point for a variety of student experiments.
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    Notes: Abstract An environmentally friendly redox laboratory for the determination of Fe(II) in an acid aqueous medium is presented. This laboratory exercise is an appropriate substitute for the traditional dichromate titration, which is environmentally problematic. This titration method uses the periodate ion as the oxidizing agent and yields results which are as accurate as the dichromate titration. Student success rate in quantitative analysis is better than 80% in two separate laboratory-based trials. This method requires a fairly high degree of operator skill to yield good results, making this a viable laboratory exercise at the sophomore or junior level.
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    The chemical educator 2 (1997), S. 1-6 
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    Notes: Abstract The drinking bird toy is modified so that it operates using ice instead of liquids. It is shown how this and other modifications can be used in general and physical chemistry courses to illustrate a variety of thermodynamic concepts. Expressions are given for the maximum work in terms of the heat of vaporization or the heat of fusion. A sample calculation is given to compare the maximum work obtainable from equal amounts of liquid water and ice.
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    Notes: Abstract Using a simple 633-nm He/Ne or diode laser, the variation of the refractive indices of four ortho-substituted dihalogenated benzenes can be obtained, demonstrating the phenomenon of polarizability. In addition, this exercise is an excellent display of the correspondence between the properties of a chemical system on the macroscopic level and the characteristics of the molecule on a microscopic scale.
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    Notes: Abstract This experiment illustrates the value of using NMR spectroscopy to study geometric isomers, and the synthetic utility of the trans effect in inorganic reaction mechanisms. The syntheses of cis- and trans-diamminedichloroplatinum(II) are carried out on a small scale, using both 14N and 15N ammine ligands. Students use 195Pt and 15N NMR spectroscopies to study the coordination complexes prepared. The 195Pt chemical shift is indicative of the metal’s coordination environment. Furthermore, the 195Pt-15N coupling constant is sensitive to the nature and geometry of the ligands bound to the metal, and this is also explored.
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    The chemical educator 2 (1997), S. 1-3 
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    The chemical educator 2 (1997), S. 1-2 
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    The chemical educator 2 (1997), S. 1-9 
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    The chemical educator 2 (1997), S. 1-3 
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    Notes: Abstract Happy Holidays! This issue closes the second volume of The Chemical Educator. The release of Volume 2, Issue 5 in November and this, Issue 6, in December allows us to have a calendar-year schedule starting with Volume 3 (1998) and henceforth. We will have six bimonthly issues in 1998 and will move our release date to near the first of the month beginning in February 1998.
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    The chemical educator 2 (1997), S. 1-8 
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    Notes: Abstract The quest for a substantial increase in the peak capacity and, therefore, in the number of compounds that can be separated in a single chromatographic run, led to the development of multidimensional techniques in high-performance liquid chromatography (HPLC). The “classical” method of collecting fractions from the initial separation and separating them further in the secondary steps is very powerful but its fully automated “online” version requires quite complex instrumentation. In contrast, development of new chromatographic media with segregated biomodal chemistries allows the 2-D separations to be achieved in a single column with standard HPLC equipment.
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  • 87
    ISSN: 1432-041X
    Keywords: Key words Epidermis ; Newt ; Metamorphosis ; βγ-crystallin superfamily ; Cornea
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    Topics: Biology
    Notes: Abstract  EP37 is an epidermis-specific protein found in the developing embryo of the Japanese newt, Cynops pyrrhogaster. Our previous study predicted the presence of genes homologous to EP37, which show temporary shared expression at the turn of metamorphosis. In this study, we isolated and characterized three cDNAs encoding novel EP37 homologues; two from the skin of an adult newt and the other from swimming larva. Conceptual translation of the open reading frames of these cDNAs predicted proteins carrying βγ-crystallin motifs and putative calcium-binding sites, both of which are features shared by the originally identified EP37 (EP37L1), as well as a spore coat protein of Myxococcus xanthus, protein S. Immunoblot analyses and immunohistochemical studies indicated that two of the EP37 proteins, EP37L1 and EP37L2, are exclusively expressed in the epidermis (skein cells) including the figures of Eberth at premetamorphic stages. During and after metamorphosis, the expression of EP37 proteins was mainly observed in cutaneous glands, and a molecular transition to the adult types of EP37, EP37A1 and EP37A2, occurred. These observations suggest that EP37 proteins play an important role in construction of integumental tissues and adaptation to the aquatic or amphibious environment.
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    ISSN: 1432-041X
    Keywords: Key words Planaria ; Pattern formation ; Pharynx regeneration ; Cell renewal ; Monoclonal antibodies
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    Topics: Biology
    Notes: Abstract  The special morphological features of freshwater planarians make them an attractive and informative model for studying the processes of regeneration and pattern formation. In this work, we investigate pattern formation and maturation of the planarian pharynx during regeneration in tail fragments. Using three monoclonal antibodies (TCAV-1, TF-26 and TMUS-13) specific for epithelial, secretory and muscle cells, respectively, we followed the sequence and timing of differentiation and maturation of these three cell types within the regenerating pharynx. Two of these monoclonal antibodies, TCAV-1 and TMUS-13, also labelled morphologically immature cells that appear to be committed to the differentiation pathway leading to their respective adult cell types. Our results show that the cells forming the new pharynx come from undifferentiated cells through proliferation and differentiation processes rather than from differentiated cells of the old stump. We describe three stages of pharynx regeneration according to the immunoreactivity shown: (1) no immunoreactivity, corresponding to the accumulation of undifferentiated cells that form the pharynx primordium; (2) immunoreactivity to TCAV-1 and TMUS-13, corresponding to the re-building of the pharynx; and (3) immunoreactivity to TF-26, corresponding to a fully mature and functional pharynx. The sequence of differentiation of these three cell types suggests that the pharynx grows by intercalation of new undifferentiated cells coming from the parenchyma between the older pharyngeal cells, in agreement with existing models of pharynx regeneration. Finally, our results suggest an intercalary model for pharynx epithelial cell renewal.
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    Development genes and evolution 206 (1997), S. 447-454 
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    Keywords: Key words Apis mellifera ; Long-germ segmentation ; Frame of pair-rule patterning ; Alternating stripe intensities ; even-skipped
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have studied the binding pattern of antibody mAB 2B8 directed against even-skipped orthologous proteins (EVE) in honeybee embryos. Primary and secondary EVE stripes form in roughly anterior-to-posterior succession; there are 8 primary and 16 secondary stripes. The most posterior primary stripes appear only after the onset of gastrulation. The secondary stripes form by a splitting of primary stripes; they demarcate the parasegmental pattern. While these findings resemble EVE expression in long-germ beetles, the honeybee differs from both beetles and dipterans by two transient pair-rule traits in the parasegmental EVE pattern: the secondary stripes in head and thorax alternate in strength, yet out of register with the Drosophila pattern, and over the whole pattern the odd-numbered stripes vanish earlier than their even-numbered counterparts. As in Drosophila, however, the strong EVE stripes coincide with the weak engrailed (EN) stripes. These findings are taken to indicate that (1) honeybee and beetles share a conserved mode of EVE stripe formation whilst Drosophila has diverged in this respect, (2) honeybee and Drosophila have diverged from the beetles in specific pair-rule traits during the parasegmental expression of both EVE and EN, and (3) some of these traits differ in the register of segment pairing and thus may reflect regulatory divergences at the pair-rule level between dipterans and the honeybee.
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  • 90
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    Development genes and evolution 206 (1997), S. 503-514 
    ISSN: 1432-041X
    Keywords: Key words Preimplantation mouse embryo ; Brefeldin-A ; Monensin ; Golgi ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The intracellular trafficking of integral membrane and secreted proteins is likely to be a key element involved in the morphogenesis and differentiation of the early mammalian embryo. In this study, we used transmission electron microscopy (TEM) to analyse the effects of brefeldin-A (BFA) and monensin, well known inhibitors of vesicular protein trafficking in somatic cells, on the structure of preimplantation mouse embryos. Both BFA and monensin distinctively altered the morphology of Golgi compartments in the blastomeres of treated morulae. BFA-treated morulae lacked recognizable Golgi complexes but possessed heterogeneous organelle clusters consisting of an abundance of smooth tubular and vesicular membrane compartments in addition to mitochondria, endosomes and lysosomes. Treatment of morulae with monensin was associated with swelling of Golgi compartments in addition to altering the morphology of mitochondria, lysosomes and the plasma membrane. BFA, and to a lesser extent monensin, inhibited cytokinesis as evidenced by the detection of binucleate blastomeres. In addition, BFA induced morulae to decompact. These latter effects have not been reported previously for these agents in mammalian somatic cell lines or other vertebrate or invertebrate embryos. These results provide the first demonstration of the structural effects of BFA and monensin on cells of the early mammalian embryo, some of which are consistent with the known actions of these agents on components of the vesicular protein trafficking system in mammalian somatic cells. This information serves as a foundation for the further use of these agents in studies of vesicular protein trafficking as an agent of preimplantation morphogenesis.
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  • 91
    ISSN: 1432-041X
    Keywords: Key words Mesoderm ; Notochord ; Brachyury ; Amphioxus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In amphioxus embryos, the nascent and early mesoderm (including chorda-mesoderm) was visualized by expression of a Brachyury gene (AmBra-2). A band of mesoderm is first detected encircling the earliest (vegetal plate stage) gastrula sub-equatorially. Soon thereafter, the vegetal plate invaginates, resulting in a cap-shaped gastrula with the mesoderm localized at the blastoporal lip and completely encircling the blastopore. As the gastrula stage progresses, DiI (a vital dye) labeling demonstrates that the entire mesoderm is internalized by a slight involution of the epiblast into the hypoblast all around the perimeter of the blastopore. Subsequently, during the early neurula stage, the internalized mesoderm undergoes anterior extension mid-dorsally (as notochord) and dorsolaterally (in paraxial regions where segments will later form). By the late neurula stage, AmBra-2 is no longer transcribed throughout the mesoderm as a whole; instead, expression is detectable only in the posterior mesoderm and in the notochord, but not in paraxial mesoderm where definitive somites have formed.
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  • 92
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    Development genes and evolution 206 (1997), S. 536-540 
    ISSN: 1432-041X
    Keywords: Key words Brain development ; Axogenesis ; Glia ; gcm ; repo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  During embryogenesis in insects, the axonscaffold of the brain is built around the embryonic foregut which separates the anlagen of the brain hemispheres. Here, we investigate this process in Drosophila and show that the major longitudinal and horizontal tracts of the embryonic brain form superficially near the interface between the foregut and embryonic brain hemispheres. We identify three types of cellular structures which might be involved in tract formation. These are rows of glial cells at the medial brain margin, cellular bridges composed of neuronal somata and the epithelial surface of the foregut itself. The close proximity to the outgrowing axons suggests that the structures at the brain-foregut interface may play a role in the morphogenesis of embryonic brain tracts in Drosophila.
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  • 93
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    Development genes and evolution 206 (1997), S. 541-545 
    ISSN: 1432-041X
    Keywords: Key words Drosophila ; Calmodulin ; Gene expression ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The expression of the Drosophila calmodulin (CAM) gene is surprisingly complex. The nervous system, which shows intense transcription in embryogenesis, contains no detectable transcripts at the end of larval life, but becomes transcriptionally active again at pupariation. The gut shows high levels of expression throughout the life cycle, except during pupal reorganization. In contrast, CAM expression in the thoracic muscles drops significantly on transition from pupal to adult life. In the testis, transcription is strongly up-regulated prior to meiosis. Growing cells show lower transcript levels than most differentiated tissues and in general, cells with intense exocytotic or endocytotic activity show the highest mRNA levels.
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  • 94
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    Development genes and evolution 207 (1997), S. 51-63 
    ISSN: 1432-041X
    Keywords: Key words Notch genes ; Gene duplication ; Gene expression ; Somitogenesis ; Gastrulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Notch genes encode transmembrane receptors that interact with numerous signal transduction pathways and are essential for animal development. To facilitate analysis of vertebrate Notch gene function, we isolated cDNA fragments of three novel Notch genes from zebrafish (Danio rerio), Notch1b, Notch5 and Notch6. Notch1b is a second zebrafish Notch1 gene. From analysis of the Notch1b sequence we argue that the various vertebrate Notch gene subfamilies encode receptors with different signalling specificities. Notch5 and Notch6 represent novel vertebrate Notch gene subfamilies. Remarkably, Notch1b lacks expression in presomitic mesoderm, Notch5 is expressed in a metameric pattern within the presomitic mesoderm whilst Notch6 expression is excluded from the nervous system. The expression patterns of these genes suggest important roles in gastrulation, somitogenesis, tail bud extension, myogenesis, heart development and neurogenesis. We discuss the implications of our observations for Notch gene evolution and function.
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  • 95
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    Development genes and evolution 207 (1997), S. 131-135 
    ISSN: 1432-041X
    Keywords: Key words Drosophila ; Embryonic development ; Antibody staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genetic dissection of any developmental processes requires mutagenesis protocols and the subsequent phenotypic screen of the established mutant strains. Whereas external structures such as the Drosophila cuticle are relatively easy to score without the need of further manipulations, the analyses of internal structures such as the nervous system often requires the use of antibodies to detect abnormalities. Here we describe an improved method to: (a) simultaneously collect Drosophila eggs from large number of fly strains, (b) process them fast for antibody staining and (c) facilitate rapid subsequent screening.
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  • 96
    ISSN: 1432-041X
    Keywords: Key words Somitogenesis ; Segmentation ; Sclerotome Kidney ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  During vertebrate embryonic development, pairs of metameric units, the somites, bud off at the cranial end of the paraxial mesoderm. The somites soon obtain cranio-caudal and dorso-ventral polarity. Establishment of dorso-ventral and medio-lateral polarity depends on multiple signals from the notochord, neural tube surface ectoderm and lateral mesoderm. The establishment of cranio-caudal polarity in the somite is less well understood. One molecule involved is the Dll1 gene product, a transmembrane protein expressed in the unsegmented paraxial mesoderm and in the caudal half of the somites. We have identified a gene, Uncx4.1, expressed in the caudal half of newly formed somites. It encodes a protein belonging to the paired-related class of homeodomain transcription factors. Uncx4.1 expression is first detected in the entire caudal half of the somites, is later down-regulated in the myotome and dermatome, and is maintained in the caudal sclerotome and its derivatives from which part of the vertebral column will form. Thus, Uncx4.1 may be involved in the establishment and maintenance of segment polarity and in vertebral column formation. Uncx4.1 is also expressed in the first branchial arch, the meso- and metanephric kidney, the central nervous system and the first digit of the forelimb, suggesting control functions of Uncx4.1 in multiple processes of embryogenesis.
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  • 97
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    The chemical educator 1 (1997), S. 1-8 
    ISSN: 1430-4171
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 98
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    The chemical educator 1 (1997), S. 1-18 
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    Topics: Chemistry and Pharmacology
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  • 99
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    The chemical educator 1 (1997), S. 1-12 
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    Topics: Chemistry and Pharmacology
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    The chemical educator 1 (1997), S. 1-9 
    ISSN: 1430-4171
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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