ALBERT

Description: We present a workflow to estimate geostatistical aquifer parameters from pumping test data using the Python package welltestpy. The procedure of pumping test analysis is exemplified for two data sets from the Horkheimer Insel site and from the Lauswiesen site, Germany. The analysis is based on a semi‐analytical drawdown solution from the upscaling approach Radial Coarse Graining, which enables to infer log‐transmissivity variance and horizontal correlation length, beside mean transmissivity, and storativity, from pumping test data. We estimate these parameters of aquifer heterogeneity from type‐curve analysis and determine their sensitivity. This procedure, implemented in welltestpy, is a template for analyzing any pumping test. It goes beyond the possibilities of standard methods, for example, based on Theis' equation, which are limited to mean transmissivity and storativity. A sensitivity study showed the impact of observation well positions on the parameter estimation quality. The insights of this study help to optimize future test setups for geostatistical aquifer analysis and provides guidance for investigating pumping tests with regard to aquifer statistics using the open‐source software package welltestpy.

Description: Article impact statement: We present a workflow to infer parameters of subsurface heterogeneity from pumping test data exemplified at two sites using welltestpy.

Description: German Federal Environmental Foundation (DBU) http://dx.doi.org/10.13039/100007636

Description: In designed experiments, different sources of variability and an adequate scale of measurement need to be considered, but not all approaches in common usage are equally valid. In order to elucidate the importance of sources of variability and choice of scale, we conducted an experiment where the effects of biochar and slurry applications on soil properties related to soil fertility were studied for different designs: (a) for a field‐scale sampling design with either a model soil (without natural variability) as an internal control or with composited soils, (b) for a design with a focus on amendment variabilities, and (c) for three individual field‐scale designs with true field replication and a combined analysis representative of the population of loess‐derived soils. Three silty loam sites in Germany were sampled and the soil macroaggregates were crushed. For each design, six treatments (0, 0.15 and 0.30 g slurry‐N kg−1 with and without 30 g biochar kg−1) were applied before incubating the units under constant soil moisture conditions for 78 days. CO2 fluxes were monitored and soils were analysed for macroaggregate yields and associated organic carbon (C). Mixed‐effects models were used to describe the effects. For all soil properties, results for the loess sites differed with respect to significant contributions of fixed effects for at least one site, suggesting the need for a general inclusion of different sites. Analysis using a multilevel model allowed generalizations for loess soils to be made and showed that site:slurry:biochar and site:slurry interactions were not negligible for macroaggregate yields. The use of a model soil as an internal control enabled observation of variabilities other than those related to soils or amendments. Experiments incorporating natural variability in soils or amendments resulted in partially different outcomes, indicating the need to include all important sources of variability. Highlights Effects of biochar and slurry applications were studied for different designs and mixed‐effects models were used to describe the effects. Including an internal control allowed observation of, e.g., methodological and analytical variabilities. The results suggested the need for a general inclusion of different sites. Analysis using a multilevel model allowed generalizations for loess soils. The results indicated the need to include all important sources of variability.

Description: Temperate forest soils are often considered as an important sink for atmospheric carbon (C), thereby buffering anthropogenic CO2 emissions. However, the effect of tree species composition on the magnitude of this sink is unclear. We resampled a tree species common garden experiment (six sites) a decade after initial sampling to evaluate whether forest floor (FF) and topsoil organic carbon (Corg) and total nitrogen (Nt) stocks changed in dependence of tree species (Norway spruce—Picea abies L., European beech—Fagus sylvatica L., pedunculate oak—Quercus robur L., sycamore maple—Acer pseudoplatanus L., European ash—Fraxinus excelsior L. and small‐leaved lime—Tilia cordata L.). Two groups of species were identified in terms of Corg and Nt distribution: (1) Spruce with high Corg and Nt stocks in the FF developed as a mor humus layer which tended to have smaller Corg and Nt stocks and a wider Corg:Nt ratio in the mineral topsoil, and (2) the broadleaved species, of which ash and maple distinguished most clearly from spruce by very low Corg and Nt stocks in the FF developed as mull humus layer, had greater Corg and Nt stocks, and narrow Corg:Nt ratios in the mineral topsoil. Over 11 years, FF Corg and Nt stocks increased most under spruce, while small decreases in bulk mineral soil (esp. in 0–15 cm and 0–30 cm depth) Corg and Nt stocks dominated irrespective of species. Observed decadal changes were associated with site‐related and tree species‐mediated soil properties in a way that hinted towards short‐term accumulation and mineralisation dynamics of easily available organic substances. We found no indication for Corg stabilisation. However, results indicated increasing Nt stabilisation with increasing biomass of burrowing earthworms, which were highest under ash, lime and maple and lowest under spruce. Highlights We studied if tree species differences in topsoil Corg and Nt stocks substantiate after a decade. The study is unique in its repeated soil sampling in a multisite common garden experiment. Forest floors increased under spruce, but topsoil stocks decreased irrespective of species. Changes were of short‐term nature. Nitrogen was most stable under arbuscular mycorrhizal species.

Description: Deutsche Forschungsgemeinschaff (DFG)

Description: Crystalline rocks can produce dangerous radiation levels on the basis of their content in radioisotopes. Here, we report radiological data from 10 metamorphic and igneous rock samples collected from the crystalline basement of the Peloritani Mountains (southern Italy). In order to evaluate the radiological properties of these rocks, the gamma radiation and the radon emanation have been measured. Moreover, since some of these rocks are employed as building materials, we assess the potential hazard for population connected to their use. Gamma spectroscopy was used to measure the 226Ra, 232Th and 40K activity concentration, whereas the radon emanation was investigated by using a RAD 7 detector. The results show 226Ra, 232Th and 40K activity concentration values ranging from (17 ± 4) to (56 ± 8) Bq kg-1, (14 ± 3) to (77 ± 14) Bq kg-1 and (167 ± 84) to (1760 ± 242) Bq kg-1, respectively. Values of the annual effective dose equivalent outdoor range from 0.035 to 0.152 mSv y-1, whereas the gamma index is in the range of 0.22-0.98. The 222Rn emanation coefficient and the 222Rn surface exhalation rate vary from (0.63 ± 0.3) to (8.27 ± 1.6)% and from (0.12 ± 0.03) to (2.75 ± 0.17) Bq m-2 h-1, respectively. The indoor radon derived from the building use of these rocks induces an approximate contribution to the annual effective dose ranging from 8 to 176 μSv y-1. All the obtained results suggest that the crystalline rocks from the Peloritani Mountains are not harmful for the residential population, even though they induce annual effective doses due to terrestrial gamma radiation above the worldwide average values. Moreover, their use as building materials does not produce significant health hazards connected to the indoor radon exposure.

Description: Published

Description: 452–464

Description: 6A. Geochimica per l'ambiente e geologia medica

Description: JCR Journal

Description: Leg 6 of CHAIN Cruise 115 began in Rio de Janeiro on 22 April 1974, and terminated in Recife on 18 May 1974. A multi-disciplinary scientific program was carried out within the Vema Channel and on the northern flanks of the Rio Grande Rise (see Figure 1). Personnel and scientific programs representing several institutions (W.H.O.I., Scripps, Lamont-Doherty) were included in the project; Brazilian observers representing PETROBRAS and the National Research Council also participated in the program.

Description: Prepared for the National Science Foundation under Grant GA-41186. Financial support for shipboard operations and most of the scientific programs during Leg 6 of CHAIN Cruise 115 was provided under National Science Foundation grant GA-41185. Seismic profiling and bathymetry were supported under O.N.R. Contract N00014-66-C-0241. Bottom current measurements received support under N.S.F. Grant No. GA-41285 to W. Patzert and to J.L. Reid (Scripps). Support for the Lamont-Doherty nephelometer program was provided under O.N.R. Contract N00014-67-A-0108-0004 and N.S.F. Grant GA-27281. Supplementary equipment items required for the transponder navigation system were provided by the Woods Hole Ocean Industry Program.

Description: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2021. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Description: In a recent study (Jozinovi\'c et al, 2020) we showed that convolutional neural networks (CNNs) applied to network seismic traces can be used for rapid prediction of earthquake peak ground motion intensity measures (IMs) at distant stations using only recordings from stations near the epicenter. The predictions are made without any previous knowledge concerning the earthquake location and magnitude. This approach differs from the standard procedure adopted by earthquake early warning systems (EEWSs) that rely on location and magnitude information. In the previous study, we used 10 s, raw, multistation waveforms for the 2016 earthquake sequence in central Italy for 915 events (CI dataset). The CI dataset has a large number of spatially concentrated earthquakes and a dense station network. In this work, we applied the CNN model to an area around the VIRGO gravitational waves observatory sited near Pisa, Italy. In our initial application of the technique, we used a dataset consisting of 266 earthquakes recorded by 39 stations. We found that the CNN model trained using this smaller dataset performed worse compared to the results presented in the original study by Jozinovi\'c et al. (2020). To counter the lack of data, we adopted transfer learning (TL) using two approaches: first, by using a pre-trained model built on the CI dataset and, next, by using a pre-trained model built on a different (seismological) problem that has a larger dataset available for training. We show that the use of TL improves the results in terms of outliers, bias, and variability of the residuals between predicted and true IMs values. We also demonstrate that adding knowledge of station positions as an additional layer in the neural network improves the results. The possible use for EEW is demonstrated by the times for the warnings that would be received at the station PII.

Description: RISE (Union's Horizon 2020 research and innovation programme, grant agreement No.821115)

Description: Published

Description: 704–718

Description: 5T. Sismologia, geofisica e geologia per l'ingegneria sismica

Description: JCR Journal

Description: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2021. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Description: Ambient-noise records from the AlpArray network are used to measure Rayleigh wave phase velocities between more than 150,000 station pairs. From these, azimuthally anisotropic phase-velocity maps are obtained by applying the Eikonal tomography method. Several synthetic tests are shown to study the bias in the Ψ2 anisotropy. There are two main groups of bias, the first one caused by interference between refracted/reflected waves and the appearance of secondary wavefronts that affect the phase travel-time measurements. This bias can be reduced if the amplitude field can be estimated correctly. Another source of error is related to the incomplete reconstruction of the travel-time field that is only sparsely sampled due to the receiver locations. Both types of bias scale with the magnitude of the velocity heterogeneities. Most affected by the spurious Ψ2 anisotropy are areas inside and at the border of low-velocity zones. In the isotropic velocity distribution, most of the bias cancels out if the azimuthal coverage is good. Despite the lack of resolution in many parts of the surveyed area, we identify a number of anisotropic structures that are robust: in the central Alps, we find a layered anisotropic structure, arc-parallel at midcrustal depths and arc-perpendicular in the lower crust. In contrast, in the eastern Alps, the pattern is more consistently E-W oriented which we relate to the eastward extrusion. The northern Alpine forleand exhibits a preferential anisotropic orientation that is similar to SKS observations in the lowermost crust and uppermost mantle.

Description: German Science Foundation (SPP-2017, Project Ha 2403/21-1); Swiss National Science Foundation SINERGIA Project CRSII2-154434/1 (Swiss-AlpArray); Progetto Pianeta Dinamico, finanziamento MUR-INGV, Task S2 – 2021

Description: Published

Description: 151–170

Description: 1T. Struttura della Terra

Description: JCR Journal

Description: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2022. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Description: To understand the seismotectonics and the seismic hazard of the study sector of the Northern Apennines (Italy), one of the most important earthquakes of magnitude Mw = 6.5 which struck the Lunigiana and Garfagnana areas (Tuscany) on 7 September 1920 should be studied. Given the early instrumental epoch of the event, neither geometric and kinematic information on the fault-source nor its fault-plane solution were available. Both areas were candidates for hosting the source fault and there was uncertainty between a normal fault with Apenninic direction or an anti-Apenninic strike-slip. We retrieved 11 focal parameters (including the fault-plane solution) of the 1920 earthquake. Only macroseismic intensity information (from 499 inhabited centres) through the KF-NGA inversion technique was used. This technique uses a Kinematic model of the earthquake source and speeds up the calculation by a Genetic Algorithm with Niching. The result is a pure dip-slip focal solution. The intrinsic ambiguities of the KF-NGA method (±180° on the rake angle; choice of the fault plane between the two nodal planes) were solved with field and seismotectonic evidence. The earthquake was generated by a normal fault (rake angle = 265° ± 8°) with an Apennine direction (114° ± 5°) and dipping 38° ± 6° towards SW. The likely candidate for hosting the source-fault in 1920 is the Compione-Comano fault that borders the NE edge of the Lunigiana graben. The KF-NGA algorithm proved to be invaluable for studying the kinematics of early instrumental earthquakes and allowed us to uniquely individuate, for the first time ever, the seismogenic source of the 1920 earthquake. Our findings have implications in hazard computation and seismotectonic contexts.

Description: Published

Description: 1465–1477

Description: 4T. Sismicità dell'Italia

Description: JCR Journal

Description: Systematic variations in the crystal cargo and whole-rock isotopic compositions of mantle-derived basalts in the intraplate Dunedin Volcano (New Zealand) indicate the influence of a complex mantle-to-crust polybaric plumbing system. Basaltic rocks define a compositional spectrum from low-alkali basalts through mid-alkali basalts to high-alkali basalts. High-alkali basalts display clinopyroxene crystals with sector (hourglass) and oscillatory zoning (Mg#61–82) as well as Fe-rich green cores (Mg#43–69), whereas low-alkali basalts are characterized by clinopyroxenes with unzoned overgrowths (Mg#69–83) on resorbed mafic cores (Mg#78–88), coexisting with reversely zoned plagioclase crystals (An43–68 to An60–84 from core to rim). Complex magma dynamics are indicated by distinctive compositional variations in clinopyroxene phenocrysts, with Cr-rich zones (Mg#74–87) indicating continuous recharge by more mafic magmas. Crystallization of olivine, clinopyroxene and titanomagnetite occurred within a polybaric plumbing system extending from upper mantle to mid-crustal depths (485–1059 MPa and 1147–1286°C), whereas crystallization of plagioclase with subordinate clinopyroxene and titanomagnetite proceeded towards shallower crustal levels. The compositions of high-alkali basalts and mid-alkali basalts resemble those of ocean island basalts and are characterized by FOZO-HIMU isotopic signatures (87Sr/86Sri = 0.70277–0.70315, 143Nd/144Ndi = 0.51286–0.51294 and 206Pb/204Pb = 19.348–20.265), whereas low-alkali basalts have lower incompatible element abundances and isotopic compositions trending towards EMII (87Sr/86Sri = 0.70327–70397, 143Nd/144Ndi = 0.51282–0.51286 and 206Pb/204Pb = 19.278–19.793). High- and mid-alkali basalt magmas mostly crystallized in the lower crust, whereas low-alkali basalt magma recorded deeper upper mantle clinopyroxene crystallization before eruption. The variable alkaline character and isotope composition may result from interaction of low-alkaline melts derived from the asthenosphere with melts derived from lithospheric mantle, possibly initiated by asthenospheric melt percolation. The transition to more alkaline compositions was induced by variable degrees of melting of metasomatic lithologies in the lithospheric mantle, leading to eruption of predominantly small-volume, high-alkali magmas at the periphery of the volcano. Moreover, the lithosphere imposed a filtering effect on the alkalinity of these intraplate magmas. As a consequence, the eruption of low-alkali basalts with greater asthenospheric input was concentrated at the centre of the volcano, where the plumbing system was more developed.

Description: Published

Description: egab062

Description: 2V. Struttura e sistema di alimentazione dei vulcani

Description: 3V. Proprietà chimico-fisiche dei magmi e dei prodotti vulcanici

Description: 4V. Processi pre-eruttivi

Description: JCR Journal

Description: High-precision vertebral bomb radiocarbon measurements likely track philopatric movements in oceanic whitetip shark Carcharhinus longimanus.

Description: This work was made possible by a Graduate Student Fellowship from the National Ocean Sciences Accelerator Mass Spectrometry (NOSAMS) lab at Woods Hole Oceanographic Institution.

Description: The Northwest Tropical Atlantic Station (NTAS) was established to address the need for accurate air-sea flux estimates and upper ocean measurements in a region with strong sea surface temperature anomalies and the likelihood of significant local air–sea interaction on interannual to decadal timescales. The approach is to maintain a surface mooring outfitted for meteorological and oceanographic measurements at a site near 15°N, 51°W by successive mooring turnarounds. These observations are used to investigate air–sea interaction processes related to climate variability. The NTAS Ocean Reference Station (ORS NTAS) is supported by the National Oceanic and Atmospheric Administration’s (NOAA) Global Ocean Monitoring and Observing (GOMO) Program (formerly Ocean Observing and Monitoring Division). This report documents recovery of the NTAS-17 mooring and deployment of the NTAS-18 mooring at the same site. Both moorings used Surlyn foam buoys as the surface element. These buoys were outfitted with two Air–Sea Interaction Meteorology (ASIMET) systems. Each system measures, records, and transmits via satellite the surface meteorological variables necessary to compute air–sea fluxes of heat, moisture and momentum. The upper 160 m of the mooring line were outfitted with oceanographic sensors for the measurement of temperature, salinity and velocity. The mooring turnaround was done by the Upper Ocean Processes Group of the Woods Hole Oceanographic Institution (WHOI), onboard R/V Ron Brown, Cruise RB-20-01. The cruise took place between January 6 and 26 2020. The NTAS-18 mooring was deployed on January 10, and the NTAS-17 mooring was recovered on January 15. Inter-comparison between ship and buoys were performed on this cruise. This report describes these operations, as well as other work done on the cruise and some of the pre-cruise buoy preparations. Other operations during RB-20-01 consisted in the acoustic communications with the Meridional Overturning Variability Experiment (MOVE) subsurface mooring array MOVE 1-13 and acoustic downloads of data from Pressure Inverted Echo Sounders (PIES) was also conducted at MOVE 1. MOVE is designed to monitor the integrated deep meridional flow in the tropical North Atlantic. Two ARGO floats were also deployed on behalf of the WHOI ARGO group. During the cruise, atmospheric measurements of aerosols, as well as radar, Lidar, radiosondes were made as part of the ATOMIC campaign. 3

Description: Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA14OAR4320158

Description: R/V Knorr Voyage 134-15, Naples to Cagliari August 11, 1988 - August 18, 1988, science navigation log, part 1

Description: R/V Knorr Voyage 134-15 Naples to Cagliari 11 August to 18 August 1988 Science Navigation Log, Part 2

Description: The following lists actual and relative positions for 2 critical pieces in the AMPHORA Site - the WRECK and the BROKEN AMPHORA. First some comments. All LAT/LON positional data are referenced to GPS. The actual positions froM the STARELLA 88 data are reliable. The actual position (only located the broken AMPHORA on that trip) from the KNORR 88 data is not reliable for reasons which are detailed in a separate note to Tom Crook. In which case, the relative positions are important. From the relative positions of the 2 wreck locations, one can safely say that there is only one wreck in that area. The broken AMPHORA is the only tie between the two coordinate systems. This is assuming that there is only one, unique broken amphora. Uchupi believes this is the case. However, the possibility that there is more than one has not been completely ruled out. Considering the proximity of the wreck to the broken amphora based on the STARELLA 88 data, it seems implausible that you would have Missed the wreck completely once you found the broken amphora. This is what preserves the possibility of more than one, at least for the Moment. More careful and detailed examination of the KNORR 88 data tracks should indicate if you indeed went through an area located relative to the broken amphora where the wreck should have been. I haven't had time to examine the data myself yet.

Description: Equations 1-4 summarize the rotor calibration used at Woods Hole Oceanographic Institution for the VACM. A discussion of the instrumental and test details used to derive these equations follows. A list of other VACM documents and related bibliography is included.

Description: Prepared for the Office of Naval Research under Contract N00014-66-C0241; NR 083-004; N00014- 74-C0262; NR 083-004; and IDOE/NSF Grant GX-29054.

Description: The 2017 Geophysical Fluid Dynamics Summer Study Program theme was Ice-Ocean Interactions. Three principal lecturers, Andrew Fowler (Oxford), Adrian Jenkins (British Antarctic Survey) and Fiamma Straneo (WHOI/Scripps Institution of Oceanography) were our expert guides for the first two weeks. Their captivating lectures covered topics ranging from the theoretical underpinnings of ice-sheet dynamics, to models and observations of ice-ocean interactions and high-latitude ocean circulation, to the role of the cryosphere in climate change. These icy topics did not end after the first two weeks. Several of the Fellows' projects related to ice-ocean dynamics and thermodynamics, and many visitors gave talks on these themes.

Description: Funding was provided by the National Science Foundation under Grant No. OCE-1829864.

Description: The WHOI ASIT Tower station located approximately 2 miles southeast of Martha’s Vineyard. The met tower platform sits approximately 11m above the water line, depending on tide level. Site is accessed using a vessel provided by WHOI (Woods Hole Oceanographic Institution). Tower is maintained by WHOI and WHOI personnel are needed to access tower.

Description: This technical manual guides the user through the process of creating a data table for the submission of taxonomic and morphological information for plankton and other particles from images to a repository. Guidance is provided to produce documentation that should accompany the submission of plankton and other particle data to a repository, describes data collection and processing techniques, and outlines the creation of a data file. Field names include scientificName that represents the lowest level taxonomic classification (e.g., genus if not certain of species, family if not certain of genus) and scientificNameID, the unique identifier from a reference database such as the World Register of Marine Species or AlgaeBase. The data table described here includes the field names associatedMedia, scientificName/ scientificNameID for both automated and manual identification, biovolume, area_cross_section, length_representation and width_representation. Additional steps that instruct the user on how to format their data for a submission to the Ocean Biodiversity Information System (OBIS) are also included. Examples of documentation and data files are provided for the user to follow. The documentation requirements and data table format are approved by both NASA’s SeaWiFS Bio-optical Archive and Storage System (SeaBASS) and the National Science Foundation’s Biological and Chemical Oceanography Data Management Office (BCO-DMO).

Description: This report was an outcome of a working group supported by the Ocean Carbon and Biogeochemistry (OCB) project office, which is funded by the US National Science Foundation (OCE1558412) and the National Aeronautics and Space Administration (NNX17AB17G). AN, SB, and CP conceived and drafted the document. IC, IST, JF and HS contributed to the main body of the document as well as the example files. All members of the working group contributed to the content of the document, including the conceptualization of the data table and metadata format. We would also like thank the external reviewers Cecile Rousseaux (NASA GSFC), Susanne Menden-Deuer (URI) Frank Muller-Karger (USF), and Abigail Benson (USGS) for their valuable feedback.

Description: When filling out the publication agreement forms for a manuscript to be published what license should I choose? Studies continue to show favorable impact of Open Access on the scholarly literature through increased dissemination and re-use.

Description: Information is presented on the structural and functional properties of phosphates in "biochemical systems. Phosphates follow four structural formation principles: (1) linkage of phosphate units via oxygen co-ordinated metal ion polyhedra, (2) establishment of chains, (3) cross linkages of chains resulting in corrugated layers, and (4) cross linkages of layers resulting in distinct three-dimensional molecular networks. One Iinkage type does not require nor exclude another Iinkage type. On the basis of this concept on the associations of phosphate tetrahedra and metal ion oxygen polyhedra, a revised molecular model for DNA is proposed. Metal ion oxygen polyhedra excercise control on the shape of the DNA, and thus may introduce the stretching of the polymer chains This can be considered the ultimate reason why a single stranded DNA will associate itself readily with another single stranded DNA resulting in a double helix. In contrast, the coupling of a single stranded DNA by itself in making a sharp bend (loop) will not take place due to the stabilization of the structure by means of the metal ion oxygen polyhedra backbone. This model also explains thefunctional properties of the nucleic acids, for instance, the oxide chains and layers will favor proton jumps, and in the presence of a differential potential, they will form proton conduction bands. The molecular organization, as introduced by the association of metal ion coordination polyhedra with the PO4 groups, plays also a significant role in membrane dynamics. Analogous to the ion co-ordination interactions of polyphosphoric acids, the fixation of metal ions at the P-O surface and of membranes will result in a distinct molecular geometry as a whole. In this way, the membranes will act as dynamic molecular sieves, whereby the mesh size and the functional characteristics of these molecular sieves is determined by the flexible interplay of metals and the individual phospholipid compounds contained in the membranes. TP (111-P) is characterized for its ability to form a co-ordination polyhedron with polyvalent cations. In this way it resembles polyphosphates and differs from 1-P and 11-P. Triphosphate exhibits two significant properties: (1) terminal chain degradation, i.e. the release of terminal PO4 groups and formation of PO3 radicals which is controlled by external electrical forces,and (2) affinity to all cations by means of metal ion oxygen polyhedra and the establishment of an exchange affinity series for all metal ions. Concerning the biosynthesis of polysaccharides, proteins, and the nucleic acids, three conditions have to be fulfilled;. (1) acitvation of the react ion partner, (2) maximum efficiency and minimum error, and (3) well-defined control of the reactions in terms of kinetics and transportation mechanism. All three requirements are most effectively executed by triphosphates. The controlled formation of the reactive P03 radicaI not only activates the reaction partner, but also eliminates by means of the phosphate formation, the OH and O groups from the reaction system and this with extraordinary efficiency and elegance. In biochemical reactions, this role is commonly exercised by ATP.

Description: Submitted to the National Aeronautics and Space Administration under Contract NSR-22-014-001. and to the American Chemical Society under Contract PRF 1943-A2.

Description: The objectives of R/V Neil Armstrong cruise AR35-04 (Fig. 1) were to survey the flanks of the Reykjanes Ridge and determine the timing, geometry and associated geophysical characteristics of the large-scale tectonic reorganizations that occurred there in the Paleogene and continue to the present (Fig. 2). The North Atlantic plate boundary between what is today the Bight Fracture Zone and Iceland, a distance of nearly 1000 km, was originally a linear orthogonally-spreading ridge that became abruptly fragmented in a stair-step fashion following a change in plate motion [Smallwood and White, 2002]. Its subsequent evolution involved the systematic and progressive removal of offsets from north to south to re-establish its original linear configuration [Hey et al., 2016; Martinez and Hey, 2017], even though this required the ridge to then spread obliquely, since the new spreading direction remained stable. These tectonic reorganizations took place within the region of influence of the Iceland “hotspot” which creates a strong gradient in mantle melting along the ridge, increasing crustal thicknesses by ~3-4 km and decreasing ridge axis depths by ~ 3000 m between the Bight Fracture Zone and Iceland [Louden et al., 2004]. A mantle gradient in melting properties (compositional and/or thermal) is presumably what results in the regional residual basement depth anomaly that extends throughout this region of the North Atlantic from the Greenland-Iceland-Faroe Ridge to south of the Bight Fracture Zone. This gradient in mantle properties with distance from the Iceland hotspot apparently had strong modulating effects on the tectonic reorganizations: the initial segment lengths and offsets appear in regional magnetic anomaly and satellite-derived gravity maps to be smaller toward Iceland and the segments evolved to re-establish the linear ridge configuration more quickly to the north [Hey et al., 2016]. As both kinematic and “hotspot” effects influence their development, the Reykjanes ridge flanks are key areas for investigating lithospheric and mantle controls on ridge segmentation, formation and elimination of transform faults and the mechanisms controlling their evolution.

Description: This work was funded by NSF grant OCE-1756760. The Marine Advanced Technology and Education program supported the participation of the MATE interns. An InterRidge Cruise Bursary supported the participation of Dr. Dominik Palgan.

Description: Following an order of Massachusetts Clean Energy Center a calibration of a LiDAR of the type WindCube V2 by Leosphere (WLS7-436) against a met mast has been performed at a test site in the US. In this report the measurement results of the LiDAR device are compared to those from calibrated cup anemometers mounted on a met mast. The aim of this comparative assessment is to convey traceability to international standards of this particular LiDAR unit. The evaluation process is based on the IEC 61400-12-1 Ed. 2 Annex L [2]. This standard describes the calibration procedure of remote sensing devices in the frame of power curve measurements on wind turbines. However, this approach generally also applies in the field of wind resource assessment, under the recommendations of MEASNET [4]. The data of the LiDAR measurement (130 m, 125 m, 95 m and 60 m) have been compared with the measured met mast data at 4 different heights (130 m, 125 m, 94 m and 58 m) during a period of 36 days (2019.08.20 – 2019.09.25) for wind speed bins between 4 – 16 m/s and for the wind direction sector 67 – 354°. In addition, measurement results of the met mast and LiDAR unit have been compared in terms of turbulence intensity, wind direction and wind shear. UL was not involved in the installation of the instruments on the mast but has gathered all relevant information. However, UL was responsible for the installation of the LiDAR unit close to the mast. It is ensured that the results presented in this report have been measured in an unbiased manner, following the best practices and to the best knowledge of the participating persons.

Description: Performance Verification Certificate for Renewable NRG Systems reference Lidar: WLS7-94 on July 2020.

Description: AWS Truepower (AWST) has been engaged by the Massachusetts Clean Energy Center (MassCEC) to develop monitoring campaign guidance in support of MassCEC’s “Metocean Initiative”. The goal of the Initiative is to “advance planning and permitting and reduce the costs of offshore wind energy in the Bureau of Ocean Energy Management’s (BOEM) designated Massachusetts Wind Energy Area (MAWEA) and the Rhode Island/Massachusetts (RI/MA) Wind Energy Area (together, the ‘WEAs’).”[1] The data collected and developed during this campaign are planned to support characterization of the WEAs’ long-term wind resource and metocean design conditions. The body of this report presents a recommended monitoring campaign framework.. The content of this document is based upon the information presented in the MassCEC Metocean Data Needs Assessment report [1], the MassCEC Metocean Initiative RFP [2], and subsequent discussions with the campaign team. The metocean campaign described here is expected to form the cornerstone of new observed public data sets developed specifically to support regional offshore wind energy development.

Description: Author Posting. © Oxford University Press, 2021. This article is posted here by permission of [publisher] for personal use, not for redistribution. The definitive version was published in Lund, S., Acton, G., Clement, B., Okada, M., & Keigwin, L. On the relationship between palaeomagnetic secular variation and excursions-records from MIS 8-ODP leg 172. Geophysical Journal International, 225(2), (2021): 1129-1141, https://doi.org/10.1093/gji/ggaa564.

Description: Palaeomagnetic secular variation (PSV) and excursion data obtained across MIS 8 (243–300 ka) from the western North Atlantic Ocean ODP (Ocean Drilling Program) sites 1060–1063 show composite high-resolution PSV records (both directions and relative palaeointensity) developed for each site and intercompared. Two methods of chronostratigraphy allow us to date these records. First, we used published results that compared the calcium carbonate records of ODP Leg 172 sediments and tuned them with Milankovich cyclicity. We also compared our palaeointensity records with the PISO-1500 global palaeointensity record that was dated with oxygen isotope stratigraphy. We prefer the PISO-1500 record to date our cores. Two excursions are preserved in our PSV records—Excursions 8α and 9α. Our revised age estimates for both excursions are 8α (236.7–239.8 ka) and 9α (283.7–286.9 ka). We have compared shipboard measurements of the two excursions with u-channel measurements of selected excursion intervals. Excursion 8α is interpreted as a ‘Class II’ excursion (local reversal) with in-phase inclination and declination changes; Excursion 9α is a ‘Class I’ excursion with 90° out-of-phase inclination and declination changes. Averaged directions (after removal of true excursional directions) and relative palaeointensity in 3 and 9 ka overlapping intervals show significant PSV directional variability over 104 yr timescales that is regionally correlatable among the four sites. A notable pattern of angular dispersion variability involves most time spent with low (∼10°) dispersion, with three shorter intervals of high (∼25°) dispersion. The relative palaeointensity variability also shows significant variability over 104 yr timescales with three notable intervals of low palaeointensity in all four records and a direct correspondence between the three low-palaeointensity intervals and the three intervals of high angular dispersion. The two magnetic field excursions occur in two of the three low-palaeointensity/high-dispersion intervals. This suggests that the geomagnetic field operates in two states between reversals, one with regular to high palaeointensity and low directional variability and one with low palaeointensity and significantly higher directional variability and excursions.

Description: Lidar is deployed on the Air Sea Interaction Tower (ASIT) offshore structure owned and operated by Woods Hole Oceanographic Institution (WHOI); Tower is approximately 2 miles south of Martha’s Vineyard, MA. Station has a walking platform at approximately 11 m MSL, with a section of lattice mast that extends from the platform to approximately 21 m MSL. The walking platform has a “diving board” extension oriented southwest, on which the lidar is deployed. The lidar sits upon a work bench mounted outboard of the southeast side of the diving board. Figure 1 illustrates the site configuration Aside from the immediate structure, the closest obstruction is Martha’s vineyard. Open ocean fetch for the southern half of the compass; Site access controlled by WHOI; additional site details attached separately.

Description: On Aug 16-17, 2018 a rhodamine dye experiment was conducted in the coastal ocean south of Martha’s Vineyard, MA. One of the experiment’s aims was to investigate the exchanges, or the absence of such, between the mixed layer and the ocean underneath over a time scale of about a day.

Description: NSF EAR-1520825

Description: We recount here a series of attempts to tag and track whales at sea without catching them. Radio transmitters of 140 Mc were attached to the backs of whales from helicopters. Tracking the tagged whales was attempted by ship and by airplane receiving systems.

Description: Submitted to the Office of Naval Research under Contract Nonr-4446.

Description: Sound sources are designed to provide subsea tracking and re‐location of RAFOS floats and other Lagrangian drifters listening at 260Hz. More recently sweeps have been added to support FishChip tracking at 262Hz. These sources must be tuned to the water properties where they are to be deployed as they have a fairly narrow bandwidth. The high‐Q resonator’s bandwidth is about 4Hz. This report documents the tuning, and provides an overview of the sound source assembly.

Description: Funding was provided by the National Science Foundation under Grant No. OCE01756361

Description: This collection includes scientific instruments and equipment and instrument manuals, catalogs, and other material that are being maintained in conjunction with the collection of oceanographic instruments and equipment presently stored in the Falmouth Technology Park offsite storage facility. Also included in the collection are a video and photographs of a traveling exhibit by the U.S. Navy on historical oceanographic instruments, The Briny Deep Discovered, that included a number of instruments loaned to the Navy by WHOI. The exhibit made its final stop at WHOI to coincide with the Institution's 75th Anniversary, and was located in the lobby of the Clark building. This collection includes scientific equipment and instrument manuals, catalogs, reports, designs, drawings and other material. List of Series: Subject Files Equipment Manuals U.S. Navy Historical Instrument Exhibit WHOI Historical Instrument collection Instrument Development Photographs Oversized Design Drawings

Description: Performance Verification Certificate for Renewable NRG Systems reference Lidar: WLS7-94 on August 2016.

Description: The lidar is re-deployed on the south shelf of the ASIT diving board platform. The site is a three legged fixed tower situated 1 NL south of Martha’s Vineyard in 17 m of water.

Description: The MBLWHOI Library’s Institutional Repository (IR) is a CoreTrustSeal certified repository. We chose to go through this process to demonstrate our commitment to quality stewardship and to be a trusted option for our researchers facing funder and publisher requirements that data be accessible, and more recently, also citable with a DOI. The Library always recommends that researchers deposit data in an appropriate subject or community repository, but there are many cases where the dataset needing a DOI does not fit that scenario. The ability to quickly and easily deposit data in a certified repository is a value added service for our users. Some funders now mandate the data must be deposited in a FAIR repository. Being a CoreTrustSeal certified repository ensures that the MBLWHOI Library’s Institutional Repository practices FAIR principles. This lighting talk will show the steps we went through to become certified, some of the hurdles and benefits, as well our current status as an application reviewer. This process enabled us to review our internal process and procedures and re-examine any outdated practices. It shined a light on places we could improve our documentation and more clearly state our policies. The importance of demonstrating our commitment to quality and the Library’s continued efforts with the repository to remain on the forefront of technology with linked open data, schema.org, etc.

Description: The Ocean Reference Station at 20°S, 85°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology, air-sea fluxes of heat, freshwater, and momentum, and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administration’s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with past cruises that have come between October and May. This cruise was conducted on the NOAA research vessel Ronald H. Brown. During the 2017 cruise on the Ronald H. Brown to the ORS Stratus site, the primary activities were the recovery of the previous (Stratus 15) WHOI surface mooring, deployment of the new Stratus 16 WHOI surface mooring, in-situ calibration of the buoy meteorological sensors by comparison with instrumentation installed on the ship, CTD casts near the moorings. Surface drifters and ARGO floats were also launched along the track.

Description: Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA14OAR4320158

Description: The Surface Ocean – Lower Atmosphere Study (SOLAS) (http://www.solas-int.org/) is an international research initiative focused on understanding the key biogeochemical-physical interactions and feedbacks between the ocean and atmosphere that are critical elements of climate and global biogeochemical cycles. Following the release of the SOLAS Decadal Science Plan (2015-2025) (Brévière et al., 2016), the Ocean-Atmosphere Interaction Committee (OAIC) was formed as a subcommittee of the Ocean Carbon and Biogeochemistry (OCB) Scientific Steering Committee to coordinate US SOLAS efforts and activities, facilitate interactions among atmospheric and ocean scientists, and strengthen US contributions to international SOLAS. In October 2019, with support from OCB, the OAIC convened an open community workshop, Ocean-Atmosphere Interactions: Scoping directions for new research with the goal of fostering new collaborations and identifying knowledge gaps and high-priority science questions to formulate a US SOLAS Science Plan. Based on presentations and discussions at the workshop, the OAIC and workshop participants have developed this US SOLAS Science Plan. The first part of the workshop and this Science Plan were purposefully designed around the five themes of the SOLAS Decadal Science Plan (2015-2025) (Brévière et al., 2016) to provide a common set of research priorities and ensure a more cohesive US contribution to international SOLAS.

Description: This report was developed with federal support of NSF (OCE-1558412) and NASA (NNX17AB17G).

Description: In recent years, German cities were heavily impacted by pluvial flooding and related damage is projected to increase due to climate change and urbanisation. It is important to ask how to improve urban pluvial flood risk management. To understand the current state of property level adaptation, a survey was conducted in four municipalities that had recently been impacted by pluvial flooding. A hybrid framework based on the Protection Motivation Theory (PMT) and the Protection Action Decision Model (PADM) was used to investigate drivers of adaptive behaviour through both descriptive and regression analyses. Descriptive statistics revealed that participants tended to instal more low‐ and medium‐cost measures than high‐cost measures. Regression analyses showed that coping appraisal increased protection motivation, but that the adaptive behaviour also depends on framing factors, particularly homeownership. We further found that, while threat appraisal solely affects protection motivation and responsibility appraisal affects solely maladaptive thinking, coping appraisal affects both. Our results indicate that PMT is a solid starting point to study adaptive behaviours in the context of pluvial flooding, but we need to go beyond that by, for instance, considering factors of the PADM, such as responsibility, ownership, or respondent age, to fully understand this complex decision‐making process.

Description: Bundesministerium für Bildung und Forschung http://dx.doi.org/10.13039/501100002347

Description: This data was collected by a team led by Kirincich as part of a Metocean monitoring campaign sponsored by the Massachusetts Clean Energy Center (MassCEC). The campaign was designed to observe key atmospheric and ocean parameters at an existing offshore platform in the proximity of the Massachusetts and Rhode Island Wind Energy Areas. The campaign supported the purchase and installation of a LIDAR wind profiler, two cup anemometers and a wind direction vane at the MVCO Air-Sea Interaction Tower (ASIT). These instruments were installed and operated by WHOI and validated by UL-AWS Truepower following a MetOcean Measurement Plan created by UL-AWST, WHOI, and the MassCEC. All instruments were installed on WHOI's offshore tower in the fall of 2016 and operated continuously, as possible, through the end of 2020. After this time the project transitioned to become the MetOcean Reference Site (MORS-1), supported by the National Offshore Wind Research and Development Consortium (NOWRDC).

Description: This zipped content contains Lidar summary data: Daily 10-minute average files from 53-200m amsl for 2021.

Description: This data was collected by a team led by Kirincich as part of a Metocean monitoring campaign sponsored by the Massachusetts Clean Energy Center (MassCEC). The campaign was designed to observe key atmospheric and ocean parameters at an existing offshore platform in the proximity of the Massachusetts and Rhode Island Wind Energy Areas. The campaign supported the purchase and installation of a LIDAR wind profiler, two cup anemometers and a wind direction vane at the MVCO Air-Sea Interaction Tower (ASIT). These instruments were installed and operated by WHOI and validated by UL-AWS Truepower following a MetOcean Measurement Plan created by UL-AWST, WHOI, and the MassCEC. All instruments were installed on WHOI's offshore tower in the fall of 2016 and operated continuously, as possible, through the end of 2020. After this time the project transitioned to become the MetOcean Reference Site (MORS-1), supported by the National Offshore Wind Research and Development Consortium (NOWRDC).

Description: Author Posting. © The Author(s), 2021. This is the author's version of the work. It is posted here by permission of Oxford University Press for personal use, not for redistribution. The definitive version was published in Toxicological Sciences 182(20), (2021): 310-326, https://doi.org/10.1093/toxsci/kfab066.

Description: Harmful algal blooms produce potent neurotoxins that accumulate in seafood and are hazardous to human health. Developmental exposure to the harmful algal bloom toxin, domoic acid (DomA), has behavioral consequences well into adulthood, but the cellular and molecular mechanisms of DomA developmental neurotoxicity are largely unknown. To assess these, we exposed zebrafish embryos to DomA during the previously identified window of susceptibility and used the well-known startle response circuit as a tool to identify specific neuronal components that are targeted by exposure to DomA. Exposure to DomA reduced startle responsiveness to both auditory/vibrational and electrical stimuli, and even at the highest stimulus intensities tested, led to a dramatic reduction of one type of startle (short-latency c-starts). Furthermore, DomA-exposed larvae had altered kinematics for both types of startle responses tested, exhibiting shallower bend angles and slower maximal angular velocities. Using vital dye staining, immunolabeling, and live imaging of transgenic lines, we determined that although the sensory inputs were intact, the reticulospinal neurons required for short-latency c-starts were absent in most DomA-exposed larvae. Furthermore, axon tracing revealed that DomA-treated larvae also showed significantly reduced primary motor neuron axon collaterals. Overall, these results show that developmental exposure to DomA targets large reticulospinal neurons and motor neuron axon collaterals, resulting in measurable deficits in startle behavior. They further provide a framework for using the startle response circuit to identify specific neural populations disrupted by toxins or toxicants and to link these disruptions to functional consequences for neural circuit function and behavior.

Description: This research was supported by a WHOI Von Damm and Ocean Ridge Initiative Fellowships to J.M.P. and the Woods Hole Center for Oceans and Human Health (NIH: P01ES021923 and P01ES028938; NSF: OCE-1314642 and OCE-1840381).

Description: 2022-06-07

Description: Data Workup From AMPHORA Site This is a list of the work to be done on the data from the AMPHORA Site. All these data are from STARELLA Leg DSL88-02. No data from Starella Leg 1 or the Knorr cruise are included in this list.

Description: R/V Knorr Voyage 134-15 Fiber Optic Mangus Lowering Observations August 1988

Description: The following lists actual and relative positions for 2 critical pieces in the AMPHORA Site- the WRECK and the BROKEN AMPHORA. First, some comments. All LAT/LON positional data are referenced to GPS. The actual positions from the STARELLA 88 data are reliable. The actual position (only located the broken AMPHORA on that trip) from the KNORR 88 data is not reliable for reasons which are detailed in a separate note to Tom Crook. In which case, the relative positions are important.

Description: Navigation Log for MV Carolyn Chouest Skerki Bank June 1997

Description: Argo data log Jason I Jaspro 88

Description: These are the observational data collected in 2017 from the North River estuary. Data files include the long-term (LT) CTD and Aquadopp measurements from April to July, the short-term (STI from April to May and STII in late July) CTD measurements, eight shipboard CTD and ADCP surveys in April, May and July, the ADV measurements in late July, the North River mid-estuary region bathymetry, and the North River discharge (from USGS measurements).

Description: National Science Foundation#1634480

Description: A collection of documentation, memos, notes and log files relating to Long Baseline Acoustic Navigation, SHARPS navigation, vehicle control and JASON ROV general information.

Description: This is a list of the work to be done on the data from the AMPHORA site. All these data are from STARELLA Leg DSLBB-02. No data from Starella Leg 1 or the Knorr cruise are included in this list.

Description: The goals of this cruise are to test the dynamic positioning system, including all sensors and interfaces. We hope to gather data under a variety of sea conditions and quantify performance. The following control configurations will be tested: 1. Automatic heading. 2. Open-loop position keeping with wind compensation . 3. Automatic track following : ARGONAV , forward cycloid only. 4. Automatic position control: ARGONAV, both cycloids. 5. Automatic track following: ARGONAV, both cyc l oi ds. 6. Automatic position control : GPS. 7. Automatic track following: GPS.

Description: Marsili Seamount Louise Jone's data log

Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bowen, J. L., Giblin, A. E., Murphy, A. E., Bulseco, A. N., Deegan, L. A., Johnson, D. S., Nelson, J. A., Mozdzer, T. J., & Sullivan, H. L. Not all nitrogen is created equal: differential effects of nitrate and ammonium enrichment in coastal wetlands. Bioscience, 70(12), (2020): 1108-1119, doi:10.1093/biosci/biaa140.

Description: Excess reactive nitrogen (N) flows from agricultural, suburban, and urban systems to coasts, where it causes eutrophication. Coastal wetlands take up some of this N, thereby ameliorating the impacts on nearshore waters. Although the consequences of N on coastal wetlands have been extensively studied, the effect of the specific form of N is not often considered. Both oxidized N forms (nitrate, NO3−) and reduced forms (ammonium, NH4+) can relieve nutrient limitation and increase primary production. However, unlike NH4+, NO3− can also be used as an electron acceptor for microbial respiration. We present results demonstrating that, in salt marshes, microbes use NO3− to support organic matter decomposition and primary production is less stimulated than when enriched with reduced N. Understanding how different forms of N mediate the balance between primary production and decomposition is essential for managing coastal wetlands as N enrichment and sea level rise continue to assail our coasts.

Description: This work was supported by the following funding sources: National Science Foundation (NSF) grant no. DEB 1902712 to LAD, JLB, DSJ, and TJM; NSF grant no. DEB 1902695 to AEG; NSF grant no. DEB 1902704 to JAN; NSF grant no. DEB 1354214 to TJM; NSF grant no. DEB 1350491 to JLB; NSF grant no. OCE 1637630 to AEG and LAD; and additional funding from the Dorr Foundation, the Department of the Interior Northeast Climate Science Center (grant no. DOI G12AC00001), and a Bullard Fellowship (Harvard University) to LAD and from the National Academies of Science, Medicine, and Engineering Gulf Research Program to JAN. Resources purchased with funds from the NSF Biological Field Stations and Marine Laboratories program (grant no. DBI 1722553, to Northeastern University) were used to generate the data for the manuscript. Initial conversations on the effects of nutrient enrichment in marshes with Scott Warren and Bruce Peterson were critical in informing the work described in the manuscript. Sam Kelsey and Jane Tucker contributed to much of the N cycling biogeochemistry; Caitlin Bauer, Frankie Leach, Paige Weber, Emily Geoghegan and Sophie Drew assisted with field work; and Joe Vineis assisted with metagenomic analysis. This is contribution 3941 from the Virginia Institute of Marine Science. The data were compiled from multiple published sources. Links to published data can be found here: https://pie-lter.ecosystems.mbl.edu/data. The sequence data used to derive figure 6 are publicly available on the MG-RAST website under project number mgp84173.

Description: The Northwest Tropical Atlantic Station (NTAS) was established to address the need for accurate air-sea flux estimates and upper ocean measurements in a region with strong sea surface temperature anomalies and the likelihood of significant local air–sea interaction on interannual to decadal timescales. The approach is to maintain a surface mooring outfitted for meteorological and oceanographic measurements at a site near 15°N, 51°W by successive mooring turnarounds. These observations will be used to investigate air–sea interaction processes related to climate variability. This report documents recovery of the NTAS-18 mooring and deployment of the NTAS-19 mooring at the same site. Both moorings used Surlyn foam buoys as the surface element. These buoys were outfitted with two Air–Sea Interaction Meteorology (ASIMET) systems. Each system measures, records, and transmits via Argos satellite the surface meteorological variables necessary to compute air–sea fluxes of heat, moisture and momentum. The upper 160 m of the mooring line were outfitted with oceanographic sensors for the measurement of temperature, salinity and velocity. Deep ocean temperature and salinity are measured at approximately 38 m above the bottom. The mooring turnaround was done on the National Oceanic and Atmospheric Administration (NOAA) Ship Ronald H. Brown, Cruise RB-20-06, by the Upper Ocean Processes Group of the Woods Hole Oceanographic Institution. The cruise took place between 14 October and 1 November 2020. The NTAS-19 mooring was deployed on 22 October, with an anchor position of about 14° 49.48° N, 51° 00.96° W in 4985 m of water. A 31-hour intercomparison period followed, during which satellite telemetry data from the NTAS-19 buoy and the ship’s meteorological sensors were monitored. The NTAS-18 buoy, which had gone adrift on 28 April 2020, was recovered on 20 October near 13° 41.96° N, 58° 38.67° W. This report describes these operations, as well as other work done on the cruise and some of the pre-cruise buoy preparations.

Description: Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA19OAR4320074.

Description: The purpose of the Brazil Basin Tracer Release Experiment is to measure diapycnal (across isopycnal) mixing and epipycnal (along-isopycnal) mixing and stirring in the deep ocean. This cruise is the fourth in the overall experiment. In the first cruise in early 1996, 110 kg of sulfur hexafluoride (SF6) were released on an isopycnal surface near 4000 meters depth in the eastern part of the basin on the flanks of the Mid-Atlantic Ridge (MAR). The location of the release was near 21.7 S, 18.4 W. The release site was over a zonal valley that leads to the MAR and is about 5000 m deep. The isopycnal surface of the release was defined as the surface on which the potential density anomaly, referenced to 4000 dbar pressure, was 45.9408 kg/m3. The release streaks and results of initial sampling in 1996 are described in Polzin et al. [1997].

Description: © The Author(s), 2004. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hayes, J. M. (2004). An Introduction to Isotopic Calculations. Woods Hole Oceanographic Institution, https://doi.org/10.1575/1912/27058.

Description: These notes provide an introduction to: • Methods for the expression of isotopic abundances, • Isotopic mass balances, and • Isotope effects and their consequences in open and closed systems.

Description: Figures 1, 2, 6, and 9 were prepared by Steve Studley. These notes derive from teaching materials prepared for classes at Indiana University, Bloomington, and at Harvard University, Cambridge, Massachusetts. I am grateful to the students in those classes and for support provided by those institutions and by the National Science Foundation (OCE-0228996).

Description: Performance Verification Certificate for Renewable NRG Systems reference Lidar: WLS7-94 on July 2019.

Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hirschberger, C., Sleight, V. A., Criswell, K. E., Clark, S. J., & Gillis, J. A. Conserved and unique transcriptional features of pharyngeal arches in the skate (Leucoraja erinacea) and evolution of the jaw. Molecular Biology and Evolution, (2021): msab123, https://doi.org/10.1093/molbev/msab123

Description: The origin of the jaw is a long-standing problem in vertebrate evolutionary biology. Classical hypotheses of serial homology propose that the upper and lower jaw evolved through modifications of dorsal and ventral gill arch skeletal elements, respectively. If the jaw and gill arches are derived members of a primitive branchial series, we predict that they would share common developmental patterning mechanisms. Using candidate and RNAseq/differential gene expression analyses, we find broad conservation of dorsoventral patterning mechanisms within the developing mandibular, hyoid and gill arches of a cartilaginous fish, the skate (Leucoraja erinacea). Shared features include expression of genes encoding members of the ventralising BMP and endothelin signalling pathways and their effectors, the joint markers nkx3.2 and gdf5 and pro-chondrogenic transcription factor barx1, and the dorsal territory marker pou3f3. Additionally, we find that mesenchymal expression of eya1/six1 is an ancestral feature of the mandibular arch of jawed vertebrates, while differences in notch signalling distinguish the mandibular and gill arches in skate. Comparative transcriptomic analyses of mandibular and gill arch tissues reveal additional genes differentially expressed along the dorsoventral axis of the pharyngeal arches, including scamp5 as a novel marker of the dorsal mandibular arch, as well as distinct transcriptional features of mandibular and gill arch muscle progenitors and developing gill buds. Taken together, our findings reveal conserved patterning mechanisms in the pharyngeal arches of jawed vertebrates, consistent with serial homology of their skeletal derivatives, as well as unique transcriptional features that may underpin distinct jaw and gill arch morphologies.

Description: This work was supported by a Biotechnology and Biological Sciences Research Council Doctoral Training Partnership studentship to CH, by a Wolfson College Junior Research Fellowship and MBL Whitman Early Career Fellowship to VAS, and by a Royal Society University Research Fellowship (UF130182 and URF\R\191007), Royal Society Research Grant (RG140377) and University of Cambridge Sir Isaac Newton Trust Grant (14.23z) to JAG.

Description: The Ocean Reference Station at 20°S, 85°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology, air-sea fluxes of heat, freshwater, and momentum, and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administration’s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with past cruises that have come between October and May. This cruise was conducted on the Chilean research vessel Cabo de Hornos. During the 2018 cruise on the Cabo de Hornos to the ORS Stratus site, the primary activities were the recovery of the previous (Stratus 16) WHOI surface mooring, deployment of the new Stratus 17 WHOI surface mooring, in-situ calibration of the buoy meteorological sensors by comparison with instrumentation installed on the ship, CTD casts near the moorings. The Stratus 17 had parted from its anchor site on January 4 2018, so its recovery was done in two separate operations: first the drifting buoy with mooring line under it, then the bottom part still attached to the anchor. Surface drifters and ARGO floats were also launched along the track.

Description: Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA14OAR4320158.

Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Doo, S. S., Kealoha, A., Andersson, A., Cohen, A. L., Hicks, T. L., Johnson, Z., I., Long, M. H., McElhany, P., Mollica, N., Shamberger, K. E. F., Silbiger, N. J., Takeshita, Y., & Busch, D. S. The challenges of detecting and attributing ocean acidification impacts on marine ecosystems. ICES Journal of Marine Science, 77(7-8), (2020): 2411-2422, https://doi.org/10.1093/icesjms/fsaa094.

Description: A substantial body of research now exists demonstrating sensitivities of marine organisms to ocean acidification (OA) in laboratory settings. However, corresponding in situ observations of marine species or ecosystem changes that can be unequivocally attributed to anthropogenic OA are limited. Challenges remain in detecting and attributing OA effects in nature, in part because multiple environmental changes are co-occurring with OA, all of which have the potential to influence marine ecosystem responses. Furthermore, the change in ocean pH since the industrial revolution is small relative to the natural variability within many systems, making it difficult to detect, and in some cases, has yet to cross physiological thresholds. The small number of studies that clearly document OA impacts in nature cannot be interpreted as a lack of larger-scale attributable impacts at the present time or in the future but highlights the need for innovative research approaches and analyses. We summarize the general findings in four relatively well-studied marine groups (seagrasses, pteropods, oysters, and coral reefs) and integrate overarching themes to highlight the challenges involved in detecting and attributing the effects of OA in natural environments. We then discuss four potential strategies to better evaluate and attribute OA impacts on species and ecosystems. First, we highlight the need for work quantifying the anthropogenic input of CO2 in coastal and open-ocean waters to understand how this increase in CO2 interacts with other physical and chemical factors to drive organismal conditions. Second, understanding OA-induced changes in population-level demography, potentially increased sensitivities in certain life stages, and how these effects scale to ecosystem-level processes (e.g. community metabolism) will improve our ability to attribute impacts to OA among co-varying parameters. Third, there is a great need to understand the potential modulation of OA impacts through the interplay of ecology and evolution (eco–evo dynamics). Lastly, further research efforts designed to detect, quantify, and project the effects of OA on marine organisms and ecosystems utilizing a comparative approach with long-term data sets will also provide critical information for informing the management of marine ecosystems.

Description: SSD was funded by NSF OCE (grant # 1415268). DSB and PM were supported by the NOAA Ocean Acidification Program and Northwest Fisheries Science Center, MHL was supported by NSF OCE (grant # 1633951), ZIJ was supported by NSF OCE (grant # 1416665) and DOE EERE (grant #DE-EE008518), NJS was supported by NSF OCE (grant # 1924281), ALC was supported by NSF OCE (grant # 1737311), and AA was supported by NSF OCE (grant # 1416518). KEFS, AK, and TLH were supported by Texas A&M University. This is CSUN Marine Biology contribution (# 306).

Description: This collection of brief "summaries of investigations" has been prepared by the members of the research staff of the Woods Hole Oceanographic Institution and this volume is a continuation of our early traditions. For the first decade or so reports of progress by individual investigators were included as an appendix to each annual report. There were only fourteen such summaries occupying less than seven pages in the 1931 Annual Report; there were but thirteen persons on the research staff at that time. With the expansion of the Institution during the World War II years it became impractical to include a comprehensive report of progress for each investigator, but the Annual Reports did continue to describe very briefly the work being done by each. With the continued expansion of the Institution the printed annual report has become more and more impersonal and the lack of a comprehensive summary of current investigations has been apparent to all. The Collected Reprints of the Institution have continued to provide a record of the scientific results obtained by our staff members, but publication delays make these at least a year out-of-date before they appear. This report is the sixth in the series of Summaries of Investigations. They are similar in style to the reports of progress included as appendices to earlier Annual Reports and a limited number of copies is available. This collection of summaries is intended not only to supplement the limited information about the scientific investigations included in the Annual Report for 1967, but also to let our friends and associates know what each individual staff member is currently studying. These summaries have been reviewed by the department chairmen but typed, insofar as possible, without editorial change, adhering strictly to the original manuscript in most cases.

Description: A very large part of the support of our research programs came from the agencies and departments of the Federal Government listed below. We wish to express our thanks to these organizations as a whole and to the executives and administrators in them who have been so helpful to us during the past year. Acknowledgment of Support Atomic Energy Commission Coast and Geodetic Survey U. S. Coast Guard Office of Naval Research Fish and Wildlife Service, Bureau of Commerical Fisheries U. S. Geological Survey National Science Foundation Naval Ship Systems Command Naval Air Systems Command National Institutes of Health National Aeronautics and Space Administration The support for our research programs provided by private foundations and organizations, individuals and the Associates of the Woods Hole Oceanographic Institution is also very much appreciated, for it enabled us to initiate projects for research or education which could not be funded through Federal agencies.

Description: The following lists actual and relative positions for 2 critical pieces in the AMPHORA Site- the WRECK and the BROKEN AMPHORA. First, some comments. All LAT/LON positional data are referenced to GPS. The actual positions from the STARELLA 88 data are reliable. The actual position (only located the broken AMPHORA on that trip) from the KNORR 88 data is not reliable for reasons which are detailed in a separate note to Tom Crook. In which case, the relative positions are important.

Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Keller, A. G., Apprill, A., Lebaron, P., Robbins, J., Romano, T. A., Overton, E., Rong, Y., Yuan, R., Pollara, S., & Whalen, K. E. Characterizing the culturable surface microbiomes of diverse marine animals. FEMS Microbiology Ecology, 97(4), (2021): fiab040, https://doi.org/10.1093/femsec/fiab040.

Description: Biofilm-forming bacteria have the potential to contribute to the health, physiology, behavior and ecology of the host and serve as its first line of defense against adverse conditions in the environment. While metabarcoding and metagenomic information furthers our understanding of microbiome composition, fewer studies use cultured samples to study the diverse interactions among the host and its microbiome, as cultured representatives are often lacking. This study examines the surface microbiomes cultured from three shallow-water coral species and two whale species. These unique marine animals place strong selective pressures on their microbial symbionts and contain members under similar environmental and anthropogenic stress. We developed an intense cultivation procedure, utilizing a suite of culture conditions targeting a rich assortment of biofilm-forming microorganisms. We identified 592 microbial isolates contained within 15 bacterial orders representing 50 bacterial genera, and two fungal species. Culturable bacteria from coral and whale samples paralleled taxonomic groups identified in culture-independent surveys, including 29% of all bacterial genera identified in the Megaptera novaeangliae skin microbiome through culture-independent methods. This microbial repository provides raw material and biological input for more nuanced studies which can explore how members of the microbiome both shape their micro-niche and impact host fitness.

Description: Funding was provided by the National Science Foundation (Biological Oceanography) award #1657808 and National Institutes of Health grants 1R21-AI119311–01 to K. E. Whalen, as well as funding from the Koshland Integrated Natural Science Center and Green Fund at Haverford College. This constitutes scientific manuscript #298 from the Sea Research Foundation.

Description: We have developed a hollow core fiber optic sensor capable of measuring dissolved methane gas in liquids using only nanoliters of sample gas. The sensor is based on an anti-resonant hollow core fiber combined with a permeable capillary membrane inlet which extracts gas from the liquid for analysis.

Description: Woods Hole Oceanographic Institution Innovative Technology Award Schmidt Marine Technology Partners G-2004-59353 NOAA Office of Ocean Exploration and ResearchNA18OAR0110354

Description: Dissociation of methane hydrates due to ocean warming releases methane, a powerful greenhouse gas, to the atmosphere. Dissociation of gas hydrates may have led to rapid and dramatic environmental changes in the past. Thus, understanding the impact of those events requires information about their timing and magnitudes. While the foraminiferal fossil record provides a powerful tool to understand past environmental conditions, seep-endemic foraminifera are unknown, which limits evaluation of seep-specific information. However, geographically widespread benthic foraminifera do inhabit seep sites, as documented widely in the literature, and may provide information useful to the understanding of past methane releases. In an effort to better understand how benthic foraminifera inhabit this chemosynthesis-based ecosystem, and if they faithfully record the methane emissions, we conducted a multipronged analysis of foraminifera associated with a gas hydrate emission site in the Arctic. Our goal was to simultaneously assess, in single representative calcareous benthic foraminiferal individuals, the cell biology, test stable carbon isotope ratio, and carbonate microstructure (e.g., wall thickness, survey for authigenic overgrowths), from samples collected south of Svalbard, or on Vestnesa Ridge, west of Svalbard). Serially, each specimen was scanned with microCT (computerized tomography) to assess test characteristics, then the test dissolved by acidification while capturing gas to measure stable carbon isotope ratio via continuous-flow mass spectrometry, and finally the remaining soft parts embedded and examined for cell ultrastructure with a Transmission Electron Microscope (TEM). TEM). Data from isotopic analyses, microCT scans and TEM imaging are presented here.

Description: This project was funded by NSF (WHOI)OCE-1634469 NSF (UFL)OCE-1634248 Norwegian Research Council223259

Description: High-frequency radar-based observations of surface currents along the east coast of Taiwan, obtained over a 50-day period in early 2017, are used to examine the occurrence, generation, and downstream advection of submesoscale eddies in the Kuroshio. Measured at an effective depth of 2 m and radial resolution of 3 km from four land-based HF radar systems spanning an 250-km along-stream distance, the surface current observations reveal the instantaneous position of the Kuroshio on hourly time scales as well as the occurrence of numerous high relative vorticity features. Vortex features with spatial scales of 5-20 km were concentrated in the first 30 km offshore, with many created at the southern tip of Taiwan on tidal timescales. Most features, with relative vorticities approaching zeta/f=1, translated northward along the coast at the speed of the Kuroshio itself and were coherent over the 250-km length of the Taiwanese coastline. Both tides and regional winds appear to influence when long-lived features form, and the offshore advection of surface waters by the vortices are observable in intermittent Satellite images of surface chlorophyll. While most features are advected northward with the current, a submarine ridge acts to impede the flow, scattering northward moving features and causing occasional southward-migrating vortices. Data Description: DESCRIPTION; The surface current observations used here were obtained from four long-range (4 MHz transmit frequency) land-based coastal radar systems, operated by the Taiwan Ocean Research Institute (TORI) and the National Taiwan University (NTU). All systems were Codar Ocean Sensors SeaSondes, with the three southern stations operated by TORI, and the northern-most station by NTU. Collected over the time period spanning February 1st to March 26th, 2017, the hourly observations of Doppler cross-spectra had a radial resolution of 3 km. Horizontal resolution was dependent on both the resolution of the measured antenna patterns (1 degree in azimuth) as well as the inherent azimuthal resolution of the radar returns themselves. DATA_PREPARATION_DESCRIPTION; Observed Doppler cross-spectra were reprocessed following Kirincich et al. (2012) using adjusted measured antenna patterns and advanced quality control metrics to estimate the radial surface currents observed at each site. Measured antenna response patterns were adjusted iteratively to reduce radar-to-radar inconsistencies defined using synthetic radials estimated from adjacent radars as well as systematic biases in mean vorticity and divergence patterns. Vector combinations of the radial surface currents, representative of the average currents over the top 2 m of the water column (StewartJoy, 1974) were estimated using power-weighed least-squares methods (Kirincich et al. 2012, Kaplan et al 2005) with a fixed horizontal averaging length-scale of 3 km, and masked for errors due to the geometric dilution of precision (GDOP) greater than 2 (Barrack, 2002). Acquisition Description: SENSOR_INFORMATION; Radio frequency interference from the ionosphere is a particular problem for the TORI and NTU radars, due to a combination of latitude and transmit frequency, causing elevated background noise during local nighttime. Returns at ranges of 90 km, the distance to the primary scattering layer within the ionosphere, are especially affected. SNR was used as an effective screening tool to isolate and eliminate data contaminated by ionospheric radio noise common in the region, adding further improvements to the radial velocity results. However, data from a 50x50 km region directly offshore of the radar site near 23deg 30' N 121deg 30' E was excised during the hours of 11 to 17 UTC each day during the observational period due to poor data returns during times of high ionospheric reflections and radio noise that resulted in poorly resolved and inaccurate vector current estimates. Using synthetic radials from adjacent HFR sites (Emery et al 2019), surface current uncertainties are estimated to be 5-10 cm/s. the west of the 2018-2019 mooring locations. The surface mooring was located at 41.0706degN 70.8177degW in 40 m of water and sampled surface vector winds, air temperature, air pressure, and relative humidity using a Vaisala WXT520 located at 2 m above mean sea level at 10 min ensemble averages, of 1 Hz data. The 2020 surface mooring also had 5 temperature-conductivity sensors (SBE37s) that sampled the oceanic water column at fixed depths below the surface of 0.6,4,6.5,10, and 20-m at 2 min increments. Finally the 2020 subsurface mooring was deployed at 41.0706degN 70.8177degW and contained a sub-surface float at 8-m below sea level in 40 m of water. The float held an upward looking Nortek Signature 1000 AD2CP that collected 2048 pings @4Hz every 20 min at 0.25 m bin depths.

Description: HF Radar observations used here were funded by Taiwan's National Applied Research Laboratories as well as the National Taiwan University. A. Kirincich was funded by the U.S. Office of Naval Research under contract #N000141712761.

Description: Time series of current velocity, water temperature, and salinity profiles, and near-bottom water depth from the 7-m, 12-m, 17-m, and 27-m site of an inner-shelf array deployed as part of the Stratification, Wind, and Waves on the Inner shelf of Martha’s Vineyard (SWWIM) study. There were 6 deployments of an array of four sites across the inner-shelf south of Martha's Vineyard, Massachusetts. The four sites are designated by nominal depth 7-m, 12-m (MVCO node), 17-m and 27-m. Each site consisted of an upwarded looking ADCP mounted on a bottom frame and a surface mooring spanning the water column with temperature and temperature/conductivity instruments. Gaps between deployments varied from 1 to 2 months. Each site was off the south coast of Martha's Vineyard, Massachusetts and the time series span for all four sites was 11 October 2006 – 5 February 2010. Sites: 7-m site was at 41.347°N 70.556°W, 0.4 km offshore in 7 - 8 m of water 12-m site was at 41.337°N 70.556°W, 1.5 km offshore in 12 m of water 17-m site was at 41.319°N 70.570W, 3.8 km offshore in 17.5 m of water 27-m site was at 41.254°N 70.592°W, 11.1 km offshore in 27.5 m of water

Description: National Science Foundation: OCE-0548961

Description: Presented at Fall AGU (ESIP Data Helpdesk), New Orleans, 11-17, December 2021

Description: BCO-DMO curates a database of research-ready data spanning the full range of marine ecosystem related measurements including in-situ and remotely sensed observations, experimental and model results, and synthesis products. We work closely with investigators to publish data and information from research projects supported by the National Science Foundation (NSF), as well as those supported by state, private, and other funding sources. BCO-DMO supports all phases of the data life cycle and ensures open access of well-curated project data and information. We employ F.A.I.R. Principles that comprise a set of values intended to guide data producers and publishers in establishing good data management practices that will enable effective reuse.

Description: Award(s): NSF #1924618