Publication Date:
2008-03-01
Description:
Long-term potentiation (LTP) at glutamatergic synapses is considered to underlie learning and memory and is associated with the enlargement of dendritic spines. Because the consolidation of memory and LTP require protein synthesis, it is important to clarify how protein synthesis affects spine enlargement. In rat brain slices, the repetitive pairing of postsynaptic spikes and two-photon uncaging of glutamate at single spines (a spike-timing protocol) produced both immediate and gradual phases of spine enlargement in CA1 pyramidal neurons. The gradual enlargement was strongly dependent on protein synthesis and brain-derived neurotrophic factor (BDNF) action, often associated with spine twitching, and was induced specifically at the spines that were immediately enlarged by the synaptic stimulation. Thus, this spike-timing protocol is an efficient trigger for BDNF secretion and induces protein synthesis-dependent long-term enlargement at the level of single spines.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4218863/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4218863/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tanaka, Jun-Ichi -- Horiike, Yoshihiro -- Matsuzaki, Masanori -- Miyazaki, Takashi -- Ellis-Davies, Graham C R -- Kasai, Haruo -- R01 GM053395/GM/NIGMS NIH HHS/ -- R01 GM053395-12/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Mar 21;319(5870):1683-7. doi: 10.1126/science.1152864. Epub 2008 Feb 28.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18309046" target="_blank"〉PubMed〈/a〉
Keywords:
Action Potentials
;
Animals
;
Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
;
Cells, Cultured
;
Dendritic Spines/*physiology/*ultrastructure
;
Glutamic Acid/metabolism
;
*Neuronal Plasticity
;
Patch-Clamp Techniques
;
*Protein Biosynthesis
;
Protein Synthesis Inhibitors/pharmacology
;
Pyramidal Cells/physiology/ultrastructure
;
Rats
;
Rats, Sprague-Dawley
;
Receptor, trkB/metabolism
;
Synapses/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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