ALBERT

Notes: We argue that the Kearney/Foreign Policy (KFP) index of globalisation is constructed by making some problematic assumptions about the measurement, normalisation and weighting of the variables included in the index. We propose alternative measurement, normalisation and weighting rules, and using these rules, recalculate the ranking of the fifty countries, using the original KFP data. Specifically, we use, in various combinations: (i) variables ‘adjusted’ for geographical characteristics of countries; (ii) statistically optimal weights obtained by principal components analysis; (iii) a normalisation rule that treats different years of observations separately. We find that the country rankings change significantly when adjusted variables are used, indicating that the original KFP index is partially measuring geographical differences between countries.

Notes: The integration of the former communist countries of central and eastern Europe into the European Union creates a dilemma for the EU's regional policy. The EU's expenditure on regional policy (its ‘active’ regional policy) has been guided by political reactions to deepening or enlarging the EU, not by a rational strategy for regional policy. In contrast, the strong EU instrument of state aid control, developed for competition policy (its ‘reactive’ regional policy) has been relatively successful in avoiding a national race of regional subsidies among the member states. We show that a shift from active regional policy to reactive, competition-oriented, regional policies is the preferred way for the established member states to handle the challenge of enlargement. At the same time, however, this shift is politically difficult for the accession countries to accept, despite the fact that this shift might prove better for them economically. This regional policy dilemma is one of the major obstacles for the full integration of the accession countries into the EU.

Notes: The rules governing trade and capital flows have been at the centre of controversy as globalisation has proceeded. One reason is the belief that trade and capital flows have massive effects on the labour market – either positive, per the claims of international financial institutions and free trade enthusiasts, or negative, per the ubiquitous protestors at WTO, IMF and World Bank meetings demanding global labour standards. Comparing the claims made in this debate with the outcomes of trade agreements, this paper finds that the debate has exaggerated the effects of trade on economies and the labour market. Changes in trade policy have had modest impacts on the labour market. Other aspects of globalisation – immigration, capital flows and technology transfer – have greater impacts, with volatile capital flows creating great risk for the well-being of workers. As for labour standards, global standards do not threaten the comparative advantage of developing countries nor do poor labour standards create a ‘race to the bottom’.

Notes: Developing countries have been increasing their participation in the formal institutions and proceedings of the multilateral trading system. A prominent example is their more frequent involvement as defendants and plaintiffs in GATT/WTO trade disputes. This paper provides an initial economic appraisal of developing country performance in the GATT/WTO dispute settlement system. We measure the economic resolution of these disputes through trade liberalisation gains, and our results suggest that developing country plaintiffs have had more success under WTO disputes than was the case under the GATT. We also document evidence on potential determinants of this success: the capacity for plaintiffs to make credible retaliatory threats and the guilty determinations by GATT/WTO panels. Finally, there is also some evidence that developing countries have recognised the importance of retaliatory threats and have responded by changing their pattern of dispute initiation under the WTO to better take advantage of the instances in which they have sufficient leverage to threaten retaliation and induce compliance with GATT/WTO obligations.

Notes: This paper evaluates Burundi's progress with trade policy reform, by comparing earlier analyses of Burundi's trade policies undertaken in the 1980s with that of the WTO's recent Trade Policy Review. Since the mid-1980s Burundi has been trying to reform its trade and macroeconomic policies against the background of continuous socio-political tensions and periodic outbreaks of violent tribal conflict. A ‘then and now’ comparison allows us to assess both the extent of the trade reforms and of the economic return to those reforms. It is evident that there has been a significant rationalisation and simplification of trade policy. Burundi has eliminated most quantitative import restrictions and reduced the average level and range of its tariffs. The scope for allocative distortions, undesirable redistributive effects and for impediments to investment and growth has been substantially reduced. However, a return to reform in terms of export growth or diversification and of overall economic growth is not discernible yet. This is unsurprising, given the scale of the economic disruption. Sustained socio-political stability, among other things, will be required to induce the investment in human and physical capital needed for a positive return to trade policy reform.

Notes: The World Trade Organisation published a Trade Policy Review of Canada in 2003. In this paper, we discuss the WTO Review and augment the discussion by presenting original data and reviewing the empirical literature. The WTO concludes that Canada's trade regime is open and transparent but maintains barriers in a few important sectors. We subject this claim to empirical scrutiny, comparing Canada's actual imports to a multilateral benchmark based on the gravity equation. We show that Canada imports about what should be expected given the size of its economy and its location. In a second benchmarking exercise, we show that Canada's anti-dumping initiations are in proportion to its imports and that Canada's exports are targeted less by other countries’ anti-dumping investigations than what might be expected based on Canadian export levels. Like many other countries, Canada has pursued trade liberalisation through the World Trade Organisation while simultaneously signing multiple regional trade agreements. Our summary of the recent literature indicates that Canada's regional trade agreements have generated more trade creation than trade diversion. Canada has also spurred imports from the least developed countries by unilaterally eliminating tariffs and quota barriers on 48 of the world's poorest countries in January 2003. We also discuss Canadian progress in opening its agriculture and clothing industries. Overall, we conclude that Canada appears committed to advancing globalisation through multilateral trade liberalisation supplemented by unilateral and bilateral initiatives.

Notes: This paper draws on Hinkle and Schiff (2003). It analyses the planned Economic Partnership Agreements (EPAs) between the EU and Sub-Saharan Africa (SSA) from a development perspective. It does not take a position on whether SSA should enter into EPAs with the EU. Rather, it starts from the notion that the process of forming EPAs is unlikely to be reversed and examines the conditions that will maximise SSA's benefits from the EPAs. If this notion is correct, then the analysis presented in the paper applies. On the other hand, Pascal Lamy, the EU Trade Commissioner, made a proposal at the May 2004 G-90 summit in Dakar that might lead to a change in the EPA process. He proposed that the G-90, a group consisting of ACP and non-ACP LDC countries, should not have to make concessions at the WTO Doha Round of multilateral trade negotiations, i.e., he proposed a ‘free round’ for the G-90. This proposal opens the door to the possibility that the same might apply to the ACP countries in the EU-ACP negotiations and that the EPA process might be reversed. The paper considers the key issues raised by the planned EPAs, their relationship to the WTO's Doha Round and the EU's Everything-but-Arms Initiative, the changes needed to make the EPAs internally consistent, the domestic reforms in SSA that would need to accompany trade liberalisation in both goods and services, and the potential effects of the EPAs on regional integration in SSA. The EPAs will pose a number of policy challenges for SSA countries, including: restructuring of indirect tax systems, reduction of MFN tariffs, liberalisation of service imports on an MFN basis and related regulatory reforms in the services sector, and liberalisation of trade in both goods and services within the regional trading blocs in SSA. The paper also finds that the EPAs provide an opportunity to accelerate regional and global trade integration in SSA. To realise the potential development benefits of the planned EPAs, two steps are essential. First, the EU must, as it has stated, truly treat the EPAs as instruments of development, subordinating its commercial interests in the agreements to the development needs of SSA. Second, the SSA countries need to implement a number of EPA-related trade policy reforms. However, the latter is far from certain, given the lack of reform momentum in SSA.

Notes: Tunisia and Egypt have both recently undertaken significant steps toward trade reform. They have committed to a partnership agreement with the European Union. Both countries have also joined the WTO and are participating in Doha Round discussions on the liberalisation of non-tariff barriers on both goods and services trade. These developments provide an interesting context within which to investigate not only the changes in welfare associated with reforms affecting the trade in goods, but also the impacts of services liberalisation. Using open-economy computable general equilibrium models for both Tunisia and Egypt, this paper explores the reasons why structural differences in these two economies imply different opportunities and challenges with trade reform and services liberalisation. The gains from eliminating barriers at the border for goods trade are significantly greater for Tunisia than Egypt. Both countries, however, gain substantially from liberalisation of foreign direct investment in services. Furthermore, economic growth is more evenly distributed across sectors than with liberalisation of trade in goods alone. In addition to reporting on the impact of alternative policies on income, output, employment and trade, sector-level effects are also considered.

Notes: This paper analyses the impact of rules of origin on patterns of trade in the context of the pan-European system of diagonal cumulation. The paper first highlights the importance of rules of origin in all preferential trading arrangements while arguing that those rules can easily lead to trade suppression and/or trade diversion. We then focus on the introduction of the pan-European system in 1997 and show evidence to suggest that the introduction of the system materially impacted on trade between the EU, and its CEFTA, EFTA and Baltic states partner countries. The main body of the paper then empirically explores the impact of the lack of cumulation in the textile industry on the countries of the Southern Mediterranean. The results suggest that rules of origin may indeed substantially constrain trade between non-cumulating countries, possibly by as much as 70–80 per cent in aggregate. While preferential trading agreements thus serve to increase intra-PTA trade through the liberalisation of trade barriers, they may also be doing so by effectively raising external barriers to trade through the use of constraining rules of origin. To the extent that they do so increases the likelihood of trade diversion and trade suppresion.

Notes: The central theme of this paper is that sustained rapid growth cannot be achieved without rapid growth in trade. A review of the experience during the past four decades offers virtually no examples of countries achieving sustained rapid growth – called miracles in this paper – without simultaneously experiencing sustained rapid growth in trade in the presence of low or high but declining barriers to trade. Simultaneously, the claim that opening to trade leads to sustained income losses is unfounded. A review of the experience of the countries that have faced stagnation or declining per-capita incomes on a long-term basis – called debacles in this paper – reveals no connection to a sustained surge in imports.

Notes: Book reviewsINTERNATIONAL MONETARY FUND, Fiscal Adjustment in IMF-Supported Pro-grams: Evaluation Report.MAGNUS BLOMSTRÖM (ed.), Structural Impediments to Growth in Japan.EDWIN M. TRUMAN, Inflation Targeting in the World Economy.

Notes: This paper discusses the potential impacts of services trade liberalisation on developing countries and reviews existing quantitative studies. Its purpose is to distill themes from current literature rather than to advocate specific policy changes. The picture emerging is one of valiant attempts to quantify in the presence of formidable analytical and data problems yielding only a clouded image of likely impacts on trade, consumption, production and welfare emerging to the point that the policy implications of results are not always clear.A central intuition would seem to be that with genuine two-sided (OECD/non-OECD) liberalisation in services that are seemingly considerably labour-intensive in delivery, the potential should be there for significant developing country gains from global liberalisation allowing full cross-border delivery. However, this picture is neither fully endorsed by available studies, neither is it explicitly contradicted. This seems to be the case for a number of reasons.One difficulty with the studies is that the conceptual underpinnings of what determines trade in services and how this trade differs analytically from that of trade in goods (if at all) is an issue prior to assessments of impacts of liberalisation of trade in services on developing countries being discussed. Key issues here are the treatment of mobility for service providers (both firms and workers), and the differing analytical structures needed to analyse individual service items (banking, insurance, telecoms, etc.). Some recent analytical work suggests that liber-alisation in some service items, such as banking, need not always yield gains, and this contrasts with quantitative studies where analytical structures mirror conventional trade in goods treatments.The discussion and measurement of barriers to service trade in both developed and developing countries is also problematic. One is talking of domestic regulation, entry barriers, portability of providers, competition policy regimes more so than only barriers at national borders, as with tariffs. Both representing and quantifying such barriers raise major difficulties, and these are also spelled out in the paper. Which barriers actually restrict trade, and which do not because they are redundant is one issue, for instance. It is also often misleading to represent barriers in simple ad valorem equivalent form. As a result, numerical modelling work on the effects of service trade barriers which is based on ad valorem equivalent modelling is often not fully convincing.In addition, individual country results vary considerably across studies in ways that it is frequently hard for outsiders to understand. Studies do, however, point towards a tentative conclusion that effects are small and positive for developed and most developing countries if FDI flow changes accompanying service trade liberalisation are excluded from the analysis, but much larger and more variable across countries if they are present. This could be taken to suggest that mode 3 GATS liberalisation (roughly captured in some studies) might be important for developing countries; but mode 4 GATS liberalisation could be even more important given large barriers to labour flows across countries. Thus, if service trade liberalisation is thought of primarily as a surrogate for improved functioning of global factor markets in which more capital flows to developing countries and more labour flows from them to developed countries, then developing countries could benefit in a major way from genuine two-sided (OECD/non-OECD) liberalisation. Developing countries fear, however, that in global negotiations on services liberalisation where there is an asymmetry of power that largely one-sided liberalisation may be the outcome, and their gains will be correspondingly limited. The paper concludes by evaluating econometric studies on linkage between services liberalisation and country growth rules, and briefly discusses some key sectoral issues in health services and transportation.

Notes: Books reviewed:DOUGLAS A. IRWIN, Free Trade Under FireBARBARA BLASZCZYK, IRAJ HOSHI and RICHARD WOODWARD (eds.), Secondary Privatisation in Transition Economies: The Evolution of Enterprise Ownership in the Czech Republic, Poland and SloveniaJAGDISH BHAGWATI (ed.), Going Alone: The Case for Relaxed Reciprocity in Freeing TradeTHEO S. EICHER and STEPHEN J. TURNOVSKY (eds.), Inequality and Growth: Theory and Policy Implications

Notes: We examine in this paper the effects of WTO Accession on policy-making and institutional reforms in transition countries. This is done by looking at the experience of those transition countries which are already Members of the WTO and/or which have recently acceded. We start by trying to distinguish between effects of accession negotiations and from those which are the results of autonomous policy initiatives. The areas of domestic policy-making which are considered in the analysis include market access, governance, government budget, structural reforms, trade and investment arrangements with regional partners and macroeconomic management. We find that no precise blueprint of accession conditions can be ascertained and argue that the WTO played a role, albeit not an exclusive one, in the process of liberalisation. We also find that the costs of WTO Accession are not negligible, but that the benefits of WTO Membership are significant in terms of improved, more predictable, market access and its stability, improved governance and a recourse to better economic policies without significant loss to government revenues.

Notes: In large measure, the voice that developing countries were able to exercise in Cancun was a result of their effective coalition formation. In this paper we present a brief overview of the various coalitions that played an important role at Cancun. The greater part of this paper focuses on one among these various coalitions: the G20 on agriculture. The G20 presents an especially fascinating case of a coalition that combined a great diversity of members and apparently incompatible interests. All theoretical reasoning and historical precedent predicted that the group would collapse in the endgame. And yet the group survived. We investigate the sources of the unity of this group and trace them to a process of learning that allowed the group to acquire certain structural features and develop strategies that helped to cement it further. While our central dependent variable is the cohesion of the G20, we also address the derivative question of the costs and benefits of maintaining such coalitions. The Cancun coalitions give us an excellent case of coalitions that managed to retain their cohesion, but also ended up with a situation of no agreement rather than a fulfilment of even some of their demands. We examine some of the causes behind the impasse in the negotiation process and suggest ways in which future outcomes could be improved.

Notes: This paper sheds light on the risks associated with currency board arrangements, referring to the severe liquidity crisis that emerged in Argentina in November 2000. The inability of the Argentinean economy to grow because of an overvalued peso and the massive borrowing needs of the government in the context of rapidly rising borrowing costs seriously undermined the credibility of the fixed-exchange rate regime. Given the widespread dollarisation of the financial sector on the liability side, Argentina had arguably little choice but to stick to the currency board. A series of measures aimed at reviving growth were implemented but with no signs of upturn in demand, increasingly distrustful international investors and growing social unrest, the country was forced into default in December 2001, putting an abrupt end to its decade long experiment with hard money. This study shows that with rigid labour markets, a lack of fiscal discipline and the absence of a natural anchor currency, Argentina was never a strong candidate for a hard peg.

Notes: For FDI to help alleviate absolute poverty and stimulate economic growth in developing countries, two conditions have to be met. First, developing countries need to be attractive to foreign investors. Second, the host-country environment in which foreign investors operate must be conducive to favourable FDI effects with regard to overall investment, economic spillovers and income growth. This paper argues that it is more difficult to benefit from FDI than to attract FDI. The widely perceived concentration of FDI in few developing countries tends to obscure that, in relative terms, various small and poor countries are fairly attractive to FDI. Yet, the mobilisation of domestic resources remains by far, more important than attracting FDI for financing investment and stimulating economic growth. Furthermore, high inward FDI is no guarantee for poverty alleviation and positive growth effects. In particular, the empirical evidence suggests that host-country conditions typically prevailing in poor countries, including weak institutions and an insufficient endowment of complementary factors of production, constrain the growth-enhancing and poverty-alleviating effects of FDI. The crux is that creating an environment in which FDI may deliver social returns will take considerable time exactly where development needs are most pressing.

Notes: Prior to the onset of the Asian financial crisis there was a deterioration in the external trade position of most countries that were affected by the Asian currency crisis. However, little is known about why this occurred. This paper aims to identify the causes of a slowdown in export growth in Malaysia. While misaligned exchange rates have been widely cited as a cause of the slowdown in East Asia; in the Malaysian context at least a vulnerability to the downturn in the electronic cycle could also be a major factor leading to poor export performance. Using the US/yen dollar rate as a proxy for exchange rate misalignment and US total new orders for electronics as a proxy for global electronics demand, cointegration analysis was used to establish the likely causes of a slowdown in Malaysia's export performance. The empirical evidence suggests that the coincidence of exchange rate misalignment with a downturn in the global electronics demand cycle was responsible for the sharp deterioration in export performance.

Notes: Two basic views can be discerned in post-mortems of Argentina's currency board: (1) that weak fiscal policy was fundamentally to blame, and (2) that the peso had become too severely overvalued for the peg to survive. This paper evaluates the evidence for these rival interpretations. The real effective exchange rate index did not indicate massive overvaluation, but this index does not capture the effects on the equilibrium rate of the ‘sudden stop’ in capital flows to emerging markets after 1998. It also understates the amount of adjustment required for Argentina to reach the equilibrium rate, because neighbour countries’ dollar exchange rates were held up by Argentina's overvaluation, as is indicated by their depreciation in 2002. Argentina was particularly vulnerable to the sudden stop because of the extreme volatility of its portfolio inflows. Fiscal policy simulations suggest that, even with a substantially improved primary balance from 1994 onwards, loss of investor confidence would still have triggered unsustainable debt dynamics once the recession began to bite after 1998. The stagnation of output and prices in Argentina created a yawning gap between the interest rate on debt and the rate of growth of nominal GDP. Had the currency been floated in, say, 1995, the real devaluation of the peso would still have pushed up the debt/GDP ratio, but higher output would have left greater scope for addressing this by running a sizeable primary surplus. Moreover, the more gradual depreciation under floating might have allowed the economy to adjust to higher debt service payments without resort to default. The IMF has criticised itself for not pressing for tighter fiscal policy in the 1990s. A more fundamental criticism would be that it was seduced by the bipolar model into complacency about adjustment to real shocks and forgetting the teachings of optimum currency area theory.

Notes: Books reviewed:Robert E. Litan, Michael Pomerleano and V. Sundararjan (eds), The Future of Domestic Capital Markets in Developing CountriesPedro-Pablo Kuczynski and John Williamson (eds), After the Washington Consensus: Restarting Growth and Reform in Latin America

Notes: Democratising the governance of the IMF will significantly improve the institution's capacity to manage crises. The implementation of a democratic framework requires a reform of the Fund's ‘quota regime,’ which mediates the distribution of voting power. An optimal reform of the quota regime that reflects the increased weight of emerging economies requires matching the number of policy objectives with the number of policy instruments. Presently, there is a classic ‘assignment problem’ whereby one policy instrument (i.e., the quota regime) is aimed at achieving three objectives (i.e., member contribution obligations, access rights, and voting rights). Three different instruments need to be adopted. Member contributions should be based on member's capacity to pay; access to resources should be based on need; and voting rights should balance the rights of creditors with the principle of sovereign equality. These reforms will enhance the Fund's legitimacy and accountability as a forum for global economic policy-making.

Notes: The issue of special and differential treatment (SDT) for developing countries in the WTO has become a source of tension in North-South trade relations. The absence of an effective SDT regime clearly contributed to the failure of the Cancún Ministerial meeting of the WTO. This paper argues for a new approach that puts the emphasis on efforts to improve the development relevance of WTO rules and create mechanisms which allow greater differentiation across WTO members in determining the applicability of WTO disciplines; complemented by non-discriminatory liberalisation of trade in goods and services in which developing countries have an export interest. The former is key in allowing the WTO to expand its reach to new ‘behind the border’ policies; and the latter is important to establishing a development dimension in multilateral trade negotiations.

Notes: Ninety samples of maize, chick-peas and groundnut seeds collected from the Egyptian market were found to be heavily contaminated by molds. Alternaria, Aspergillus, Cladosporium, Eurotium, Fusarium, Mucor, Penicillium and Rhizopus were the most common fungal genera isolated from nondisinfected seeds. Aspergillus alutaceus, A. flavus, Fusarium verticillioides and F. oxysporum were isolated from all surface-disinfected seeds and were reported to produce ochratoxin A, aflatoxin B1 and zearalenone, respectively. Irradiation at a dose 4.0 kGy reduced the mold growth greatly relative to unirradiated controls. There was no growth at dose 5.0 kGy. On the basis of the radiation survival data, the decimal reduction values D10 for A. alutaceus, A. flavus and F. verticilliodies were 0.70. 2.10 and 0.93 kGy in maize. A dose of 5 kGy inhibited the toxigenic molds and mycotoxin formation in seeds. Aflatoxin B1 and ochratoxin A were detected in maize and chick-peas, whereas zearalenone was detected in maize samples. Application of radiation at a dose of 6.0 kGy detoxified aflatoxin B1 by 74.3–76.7%, ochratoxin A by 51.3–96.2% and zearalenone by about 78%.

Notes: Fresh-cut cantalcupe has been recalled due to the possible presence of Listeria monocytogenes. Several studies have reported that naturally occurring microflora of vegetable surfaces may be antagonistic to pathogen attachment, growth or survival. To test this possibility for L. monocytogenes and cantaloupes, whole melon were treated with water, ethanol (70%) or chlorine (200 ppm) to reduce the native microflora on the melon surfaces. Treated or untreated melons were immersed in a six strain cocktail of L. monocytogenes (107 CFU/mL) for 10 min and then allowed to dry for 1 h inside a biosafety cabinet followed by storage at 5, 10 and 20C for 15 days. Fresh-cut pieces prepared from the treated or untreated melons and directly inoculated with L. monocytogenes (3.48 log CFU/g) were stored under the same conditions listed above. Populations of L. monocytogenes and five classes of native microflora were investigated. Growth of L. monocytogenes in sterile or nonsterile rind and fresh-cut homogenates was also studied. The population of L. monocytogenes recovered from inoculated (103 to 108 CFU/mL) whole melons given no disinfection treatment or washed with water was significantly less (P 〈 0.05) than that recovered from melons treated with chlorine or EtOH. In general, populations of L. monocytogenes declined on the surface of treated and untreated whole melons and on fresh-cut pieces over the 15 days storage period at the temperatures tested. However, the decline in pathogen populations was less rapid in the presence of reduced populations of native microflora. Higher populations of L. monocytogenes were attained in sterile tissue homogenates than in nonsterile homogenates. Addition of yeast and mold to sterile rind homogenates was highly inhibitory to growth and survival of the pathogen. The results of this study indicate that native microflora of whole cantaloupe inhibited attachment to rind surfaces as well as survival and growth of L. monocytogenes on cantaloupe surfaces and homogenized fresh-cut pieces. Thus, L. monocytogenes recontamination of melons having a reduced level of native microflora following application of a disinfection treatment may be a food safety concern.

Notes: Streptococcus pyogenes is widely recognized as a human pathogen. Whereas person-to-person transmission is the most common transmission mechanism for this pathogen, some outbreaks of S. pyogenes disease have been reported to occur in association with consumption of contaminated foods such as shrimp or potato salads. In this study, the behavior of S. pyogenes was studied in mashed potatoes as a function of storage temperature, types and amount of background biota and type of ingredients. Combined mashed potatoes (potatoes, butter, milk, egg and table salt) or plain mashed potatoes (potatoes only) were inoculated with a 5-strain cocktail of S. pyogenes and stored at 7, 25, 35 or 37C. At intervals during storage, samples were collected for counting S. pyogenes in blood agar plates or blood agar added with sodium azide, polymyxin and crystal violet. Mashed potatoes obtained from fast-food restaurants were used to determine the fate of S. pyogenes as affected by changes in aerobic mesophiles, coliform and lactic acid bacteria counts. S. pyogenes was able to survive in mashed potatoes stored at 7C and to grow in mashed potatoes stored at 25 or 37C with lag phase lengths of 3 and 2 h and generation times of 26.0 and 25.3 min, respectively. The generation time of S. pyogenes in plain mashed potatoes was 30.7 min at 35 C. Presence of active background biota at 2–3 log10 CFU/g concentrations did not prevent growth of S. pyogenes when stored at 35C. These results contribute to a better understanding of the potential for S. pyogenes to cause foodborne outbreaks.

Notes: Survival of stationary phase Listeria innocua (as surrogate microorganism for L. monocytogenes) inoculated in apple juice (pH 3.3 or 3.8) supplemented with vanillin (1,500 ppm or 3,000 ppm) or potassium sorbate (500 ppm or 1,000 ppm) and stored at room temperature was studied. L. innocua survived in apple juice without the preservatives at pH 3.3 or 3.8, with minimal population reductions. In the juices with the incorporation of potassium sorbate or vanillin, L. innocua behavior depended on the pH value, the type of antimicrobial and its concentration. At pH 3.3, the presence of vanillin (3,000 ppm) or potassium sorbate (1,000 ppm or 500 ppm) decreased L. innocua counts, with population reductions ranging from 4 to 5 log cycles after a 4 h – 8 h exposure at 30C. However, at pH 3.8, L. innocua showed sensitivity only to 3,000 ppm vanillin. Survival curves were successfully fitted using a Weibull type distribution of resistances. The results suggest that the use of potassium sorbate or vanillin could prevent the survival of L. innocua in contaminated unpasteurized and pasteurized apple juice. Vanillin, a natural antimicrobial, would be particularly suitable as an antilisterial additive for less acidic apple juice.

Notes: To monitor the bacteriological quality of raw milk and raw milk farm products, 143 samples of raw farm milk and 100 samples of raw milk farm products, 64 butters, 9 yogurts, 16 cheeses, 7 ice creams and 4 fresh cheeses, produced in Belgium were examined for coliforms, β-glucuronidase positive Escherichia coli, verotoxin producing Escherichia coli O157, Staphylococcus aureus, Salmonella spp., Listeria spp. and Listeria monocytogenes. The results were compared with the threshold and maximum values of the EC directive 92/46/EC or the maximum values of the Belgian Order of Council from September 3, 2000. The presence of staphylococcal enterotoxins was investigated on samples with S. aureus counts higher than the legal threshold values mentioned in the EC directive or, if not regulated in the directive, higher than the maximum value mentioned in the Belgian Order of Council. The obtained results for the hygiene-indicators coliforms, β-glucuronidase positive E. coli and S. aureus in the raw milk samples were comparable with most other industrialized countries. Compared to a prevalence of 0.7% and 6.3% for, respectively, E. coli O157 and L. monocytogenes, no Salmonella was found in the 25 g raw milk farm samples. The isolated E. coli O157 strain was confirmed to be verotoxigenic; it was positive for VT2, eaeA and hlyA. In butter not only a prevalence of 18.7% for L. monocytogenes in 25 g was found but also the maximum values for the hygiene-indicators mentioned in the Belgian Order of Council were often exceeded. No significant difference was found between the count of hygiene-indicators and the presence of Listeria spp. as well in raw milk as in raw milk butter. The bacteriological quality of on-farm made raw milk butter suggest that suitable hygienic conditions are not always provided. One of the 7 ice cream samples contained L. monocytogenes in 25 g.

Notes: Judges were required to rate the total intensity of NaCl solutions using a variety of unstructured category and line scales under a ‘rank-rating’ protocol and a traditional protocol that did not allow retasting or the reviewing of scores. The various scales and protocols induced two types of scaling errors. The first type was named a different-stimulus error. This involved a judge rating a stronger stimulus as equal to or less than a weaker stimulus. The second type was named a same-stimulus error. This involved a judge giving different ratings to two stimuli of equal concentration. For all scales, judges made a higher proportion of same-stimulus errors than different-stimulus errors. ‘Rank-rating’ only reduced the proportion of different-stimulus errors. It was hypothesized that a category scale with fewer categories would induce a higher level of different-stimulus errors but lower level of same-stimulus errors. These trends were noted but not always significantly. For line scales, the equivalent hypotheses regarding line length were either weak or not supported. It would appear that increasing the length of a line scale is not always equivalent to increasing the number of categories in a category scale.

Notes: The assessor performance is a key point in a sensory evaluation. In particular, at the end of a session, a decrease of the performance can be feared. We propose to analyze this performance with various criteria: usual ones as the main product effect or the error variance; a new one measuring the perceived products variability. The performance can then be studied all along the session from two points of view: in taking into account the only products tested at a given instant (named instantaneous); in taking into account all the products tested up to a given instant (named cumulative). In the presented example, in spite of the large number of products successively tested by each assessor, the instantaneous performance of the panel shows no significant deterioration. Furthermore, when the number of products tested by each assessor increases, more significant product effects can be obtained thanks to the accumulation of the amount of data. This shows that the number of products that can be reasonably studied by one assessor during one session is generally underestimated.

Notes: Two experimental procedures recommended for the evaluation of the psychophysical characteristics of odorous compounds, olfactory matching with the 1-butanol scale and cross-modality matching with the finger span are compared. The intensity of ethyl butyrate and guaiacol solutions presented at four different concentration levels was evaluated by a panel of sixteen subjects over five repetitions using the two methods. Each stimulus was delivered to the subject from a Teflon bag through a nose-shaped glass sniffing port.The discrimination ability, repeatability, panel homogeneity and within-subject variability of the methods were assessed. Results indicate that with both methods, subjects were able to highly discriminate the presented concentration levels of the odorants. There were no great difference in repeatability and the same individual variability was observed between both methods. However, the smaller within-subject variability highlighted for the 1-butanol scale method suggested that this method is potentially more powerful than finger-span method.

Notes: People can eat a food without having a strong preference for it, and people can prefer a food without eating it. Given this seeming disconnect between attitude and behavior, which type of measure or segment can best be used to profile or identify loyal consumer segments of a food, such as soy? This research compares a usage-based method (heavy-light-nonusers) with a new attitude-based method (seeker-neutral-avoider), and finds that the attitude-based method differentiates purchase-related intentions better than the usage-based method. Implications for profiling consumer taste patterns and consumer segments are provided.

Notes: Astringent and bitter sensations are characteristic sensory qualities of black tea. Three different classes of potential astringent reference standards (two concentrations each of alum and tannic acid and three fruit juices) were evaluated in this study. The perceived astringency, bitterness and sourness of each were profiled using computerized time-intensity and compared with the astringent intensity of a standardized brew of black tea. The differences in temporal profiles of potential reference standards across taste attributes were evident and intensity ratings were found to be dependent upon the stimulus and its concentration. Both concentrations of tannic acid were evaluated as the highest in perceived bitterness. For the juices, a strong sour taste was perceived in addition to astringency. It was concluded that the best reference standard for the astringency of black tea is a solution of 0.7 g/L alum as it is low in perceived bitterness and sourness.

Notes: We developed an alternative method for the determination of 6-n propylthiouracil (PROP) taster status that is based on the comparison of intensity ratings of PROP solutions with intensity ratings of visual, tactile, and kinesthetic (physical) stimuli. We then compared the classification obtained with this method to those derived from traditional approaches. One hundred and forty-two subjects rated the perceived intensity of 0.000032, 0.00018, 0.00032, 0.0018, and 0.0032 M PROP, and 0.01, 0.032, 0.1, 0.32, and 1.0 M NaCl, as well as five levels each of perceived roughness of sandpaper, grayness of shades, and thickness of honey and water mixtures on the Labeled Magnitude Scale (LMS). Ratings for 0.00032 M PROP and 0.1 M NaCl were also compared in a one solution test. Subjects were classified as PROP nontasters, tasters or supertasters by all three procedures. Seventy-six percent of panelists were classified in a similar way by the physical stimuli and NaCl procedures, while 70% percent of panelists were classified similarly by the physical stimuli procedure and the one solution test, as well as by the NaCl procedure and the one solution test. Only 65% of panelists were classified similarly by all three procedures. Further, in replicate ratings of PROP solutions, supertasters’ evaluations of PROP at all concentration levels decreased significantly (P 〈 0.01) from the first to the second rating. It is evident from these findings that the method used to determine PROP taster status can greatly influence the outcome of any investigation of PROP taster status on other phenotypes.

Notes: The objective of this study was to verify key aroma-active compounds responsible for reconstituted fresh skim milk powder (SMP) aroma using threshold analysis, odor activity values, and model systems. Twelve odor-active compounds of SMP and one odor-active compound from fluid milk were selected based on flavor dilution factors from gas chromatography-olfactometry. Thresholds for the 13 odor-active compounds were identified using five-set ascending forced choice threshold analysis in odor-free water and fluid skim milk. Model systems were prepared using rehydrated milk retentate (RMR). The aroma of each model was evaluated by descriptive sensory analysis and by difference-from-control testing using a trained panel. The aroma of reconstituted fresh SMP and liquid skim milk were used as controls. Models containing a mixture of twelve of the thirteen chemicals had the most similar odor characteristics to rehydrated SMP aroma (9.0/10) indicating that these compounds constitute the character impact odorants of rehydrated fresh SMP.

Notes: The sensory characteristic “beany” is a limiting factor in introducing soy products to many consumers in western cultures. This research described sensory properties of beany flavor and described sensory odor characteristics of 19 chemicals that have been associated with beany aroma and flavor in previous literature. Seven concentrations varying from 1 to 100,000 ppm of each chemical (if soluble in propylene glycol) were examined by a five member trained descriptive panel. The panel described beany as having multiple attributes. Musty/earthy, musty/dusty, sour aromatics, and characterizing attributes such as green/pea pod, nutty, or brown collectively imparted beany aroma and flavor of most beans. Starchy flavor and powdery texture also were intrinsically associated with beany in bean product samples. Some chemicals tested were not beany at any level tested. Three alcohols, two ketones, one aldehyde, and one pyrazine exhibited beany characteristics (green/peapod or brown) only at low concentrations, generally 1-10 ppm.

Notes: This paper deals with the analysis of drivers for self-defined craveability assessed in an Internet-based, conjoint analysis task. The stimuli comprised 36 descriptions of restaurant hamburgers, including product features, benefits, restaurant names, and emotional reactions that might ensue after eating the hamburger. Elements were combined into concepts by experimental design, and the resulting concepts evaluated by 145 respondents, on the attribute of craveability. Models relating the presence/absence of concept elements to ratings revealed that statements about the hamburger itself were the most powerful, but that no single element was highest across all the respondents. Segmentation of the 145 individuals by the pattern of their individual utilities revealed four key segments. These are Elaborates who may be sensory-oriented and respond strongly to product descriptions; Classics who like the notion of a grilled hamburger; Imaginers who respond to restaurant name and advertising copy; and Emotionals who respond to statements about food to descriptions how the eater feels after consumption.

Notes: Judges were required to rate the total intensity of NaCl solutions and the sweetness of orange flavored beverages using a variety of unstructured category and line scales. Discrimination errors were noted for each type of scale. No strong differences were observed in discrimination between category and line scales although a 20-point category scale incurred fewer discrimination errors than 9-point category and line scales. Allowing judges to retaste stimuli and review and modify their scores reduced discrimination errors.

Notes: The objectives of this study were to compare oral and nonoral sensory evaluation for discrimination of texture of acid skim milk gels and to establish whether nonoral attributes could be correlated to the oral perception of texture. Trained panelists (n = 13) identified 11 nonoral (visual and in-hand) and 4 oral attributes during a preliminary profiling session that could be used to discriminate textures (P 〈 0.001) in a range of acid gels prepared with different solid contents and heat treatment of the milks. Both methods of sensory appraisal were found to discriminate between gels. Correlation analysis showed high interrelationship between individual oral and nonoral attributes (P 〈 0.01). Principal component analysis revealed that all 4 oral attributes could be combined into one single attribute (PC1), with equal relative importance of the individual attributes in explaining the variance in the oral sensory data set. Canonical correlation analysis revealed good correlation between the oral and nonoral set of attributes (R2 〉 87.5%).

Notes: : This study examined the effects of location within a package and display time on the incidence of premature browning in patties made from ground beef packaged in a high-oxygen modified atmosphere (80% oxygen and 20% carbon dioxide) or in vacuum for up to 14 d. On days 3, 5, 8, 10, 12, and 14 of display, patties were formed from both top and bottom portions of tissue exposed to high-oxygen. These patties contained predominantly oxymyoglobin and metmyoglobin at the time of formation and cooking. As display progressed, the percentage of oxymyoglobin decreased (P 〈 0.05) and metmyoglobin increased (P 〈 0.05) in the patties for both locations, whereas the percentage of deoxymyoglobin remained stable at less than 2%. Samples that were vacuum-packaged contained predominantly deoxymyoglobin. Patties from high-oxygen packages cooked to a temperature of 71.1 °C were prematurely brown (P 〈 0.05) compared with vacuum-packaged samples. Patties containing predominantly deoxymyoglobin remained pinker than patties cooked directly from high-oxygen packages (lower visual color score, higher a* value; P 〈 0.05) on the interior after cooking. Data in this study clearly demonstrate that, regardless of time in display, nearly 100% of patties formed from high-oxygen-packaged ground beef would be prematurely brown when cooked and could pose a food safety problem if not cooked to an endpoint temperature ensuring safety.

Notes: : A recently reported, miniaturized method can simultaneously enumerate 4 critical indicator microorganisms in 24 h on a single 96-well microtiter plate, and is a convenient monitoring system for ensuring food plant hygiene. However, the utility of this method is limited by the necessity of plates being freshly prepared prior to each use. This study was undertaken to develop a method that would permit these plates to be prepared and stored in a stable form that could be conveniently used later. A microtiter plate filled with media dedicated to enumerating 4 specific classes of bacteria was freeze-dried. A given series was dedicated to enumerating either, total mesophilic bacteria, Gram-negative bacteria, coliform bacteria, or Escherichia coli. Freeze-dried plates were reconstituted simply by adding 100 μL of sterile water to each well. After adding samples, two-fold serial dilutions were performed, and the plate was incubated for 24 h at 32 °C. Growth of 4 indicator microorganisms was detected in each series using metabolic indicators. The numerical estimates that these procedures yielded correlated very closely with numbers gained from conventional spread-plating methods (r2 〉 0.90). Additionally, the estimates obtained from liquid media microtiter plates and freeze-dried media plates showed a significantly strong relationship (r2 〉 0.92). Analysis of commercial ground beef showed a highly associated relationship (r2 〉 0.94) between the liquid four-culture and freeze-dried four-culture plate methods. The greatly enhanced facility of using premanufactured, freeze-dried microtiter plates would make this a most convenient way to perform microbial food quality analysis.

Notes: : Electron beam irradiated sliced cantaloupe was tested for 21 d of storage for total aerobic microbial counts, texture, color, and different sensorial parameters as a function of irradiation doses 0, 0.7, and 1.4 kGy and the wash treatments, 1 and 200 ppm chlorine applied to the melons before cutting. Irradiation resulted in a reduction in the total aerobic microbial counts with increasing doses. Melons washed only with water before cutting had total aerobic bacterial counts of 4.0, 2.0, and 0.8 log colony-forming units (CFU)/g on day 0 at irradiation doses of 0, 0.7, and 1.4 kGy, respectively. Across all doses of irradiation, counts were consistently lower for cantaloupe pieces obtained from melons that had been subjected to chlorine rinse in comparison with those washed with water without chlorine. Melons washed with chlorine before cutting had total aerobic bacterial counts of 2.7, 0.7, and 0.5 log CFU/g on day 0 at irradiation doses of 0, 0.7, and 1.4 kGy, respectively. Objective color analysis indicated no significant effect of irradiation on the color of cantaloupe. Texture measured as compression force was lower only for cantaloupe irradiated at 1.4 kGy. Irradiation did not affect descriptive attribute flavor and texture sensory attributes of cantaloupe pieces. Decontamination of whole cantaloupes before cutting using chlorine wash may be combined with low-dose irradiation for shelf-life extension of sliced cantaloupe.

Notes: : Magnesium is bound as the central atom of the porphyrin ring of the green plant pigments chlorophyll a and b. It has been suggested that chlorophyll-bound magnesium may play an important role in magnesium nutrition because when iron is similarly bound in the porphyrin ring of heme, it is absorbed to a greater extent than non-heme iron. We have analyzed 22 frequently consumed fruits and vegetables for the chlorophyll content by high-pressure liquid chromatography and for magnesium with atomic absorption spectroscopy. Chlorophyll concentrations ranged from 6 μg/g (grape) to 790 μg/g (spinach) (median 63 μg/g). Magnesium concentrations ranged from 48 μg/g (grape) to 849 μg/g (spinach) (median 122 μg/g). In the green leafy vegetables, such as spinach and lettuce, chlorophyll-bound magnesium represented 2.5% to 10.5% of total magnesium whereas other common green vegetables, pulses and fruits contained 〈 1% chlorophyll-bound magnesium. The chlorophyll content of spinach was further decreased by about 35% on thawing frozen spinach or on chopping fresh spinach, and this degradation increased to about 50% after boiling and steaming. Based on the present results and published food consumption data, we estimate that chlorophyll-bound magnesium represents a very low fraction of total magnesium intake in industrialized countries, less than 1% in the case for data obtained from Switzerland. Thus, chlorophyll-bound magnesium is of little relevance to magnesium nutrition.

Notes: : Oxidative stress induced by reactive oxygen species may be linked to neurodegenerative diseases. Fresh Red Delicious apples, having 232.9 mg/100 g vitamin C equivalent antioxidant capacity, protected the rat pheochromocytoma neuronal (PC-12) cells from H2O2-induced oxidative toxicity in vitro in a dose-dependent manner. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) reduction assay showed significant increase in cell viability when PC-12 cells were treated with apple extracts. This indicates that the apple phenolics protected oxidative stress-induced neurotoxicity. Because oxidative stress is also known to increase neuronal cell membrane breakdown, we further investigated by lactate dehydrogenase and trypan blue exclusion assays. Apple phenolics inhibited oxidative stress-induced membrane damage in neuronal cells. Therefore, these results may suggest that naturally occurring antioxidants, such as phenolic phytochemicals in fresh apples, may reduce the risk of neurodegenerative disorders.

Notes: : The impact of label information on the liking and closeness to ideal of tomato juice beverages was examined by having 100 judges assess 3 tomato juice beverages (Campbell's tomato juice, V8 juice, and an experimental tomato juice enhanced with soy) either with or without labeling information. Judges rated overall liking of each product and then rated appropriateness of various attributes (saltiness, tomato flavor, thickness, texture, red color, orange color, and brown color) on 5-point just-right scales. Only half of the judges were presented with product-related label information during evaluation. Overall liking scores were analyzed using repeated-measures ANOVA, whereas different attribute ratings were analyzed using Thurstonian Ideal Point modeling and Chi-square. Product-associated label information did not significantly alter overall liking ratings (P 〉 0.05), although a significant difference in liking was found between products (P 〈 0.05). Similarly, the label information did not impact comparison of product attribute levels to ideal attribute levels. Both V8 and Campbell's were significantly different from the ideal for 3 of the attributes (P 〈 0.05). For the soy-enhanced tomato juice, all 7 attributes were significantly different from the ideal (P 〈 0.05). In this instance, labeling information had no notable impact on assessments.

Notes: : The effects of genotype, root size, storage, and processing on the content of bioactive compounds in carrots were determined to investigate the possibilities for optimizing the health-promoting properties of this vegetable. The content of polyacetylenes (falcarinol, falcarindiol, falcarindiol-3-acetate), carotenoids (α-and β-carotene), and isocoumarin 6-methoxymellein (6-MM) varied significantly between 6 genotypes of the Nantes type. The content of falcarindiol, falcarindiol-3-acetate, and 6-MM was significantly higher in small (50- to 100-g root size) than in extra large root sizes (〉250-g root size). Refrigerated storage of the roots for 4 mo at 1 °C before processing resulted in a significantly higher content of polyacetylenes and no differences in the content of carotenoids and 6-MM compared with frozen storage of processed carrots. The content of falcarinol increased and that of falcarindiol and falcarindiol-3-acetate decreased during steam blanching of the carrots before freezing. No changes were observed in the content of carotenoids and 6-MM.

Notes: : The dairy beverage market is a competitive and growing category in the food industry. Within this arena, chocolate milks vary widely in flavor, color, and viscosity. Understanding what sensory properties drive consumer liking is critical for maximum market share. This study was conducted to identify and define sensory characteristics of commercial chocolate milks and to link these differences to consumer preferences through the application of internal and external preference mapping. A sensory language was identified to document the sensory properties (visual, flavor, mouthfeel) of chocolate milks. Twenty-eight commercial chocolate milks were subsequently evaluated by descriptive sensory analysis using the identified sensory language. Thirteen representative milks were chosen for consumer acceptance testing followed by internal and external preference mapping to identify key drivers. Instrumental color and viscosity measurements were also taken. Two different techniques were used for external preference mapping: cluster analysis with generalized procrustes analysis and landscape segmentation analysis. Chocolate milks were differentiated by descriptive sensory analysis (P 〈 0.001). Wide variability was also observed in consumer acceptability of chocolate milks (P 〈 0.001). Correlations were observed among descriptive and consumer, descriptive and instrumental, and instrumental and consumer results (P 〈 0.001). Generalized procrustes analysis revealed 2 groups of consumers with 1 nondistinguishing driver of liking: cocoa aroma. Landscape segmentation analysis confirmed and clarified generalized procrustes analysis results by identifying 3 consumer segments with 3 drivers: cocoa aroma, malty, and cooked/eggy flavors.

Notes: : Pasteurized fluid milk samples were systematically collected from 3 commercial dairy plants. Samples were evaluated for microbial, chemical, and sensory attributes throughout shelf life. In general, product shelf lives were limited by multiplication of heat-resistant psychrotrophic organisms that caused undesirable flavors in milk. The predominant microorganisms identified were Gram-positive rods including Paenibacillus, Bacillus, and Microbacterium. Principal component analysis of sensory data collected using quantitative descriptive analysis showed that attributes related to milk flavor defects explained the largest amount of variance. These findings highlight the need to develop specific strategies for excluding bacterial contaminants from milk to further extend product shelf lives.

Notes: : In this study, pulsed electric field (PEF) treatment of beer, effectiveness of PEF treatment on microbial inactivation, effects of PEF treatment on sensory properties, and detection of electrode material migration were explored. Beer samples were treated by PEF for the inactivation of natural flora and inoculated cultures of Saccaromyces uvarum, Rhodotorula rubra, Lactobacillus plantarum, Pediococcus damnosus, and Bacillus subtilis. Inactivation induced by the PEF treatment was 0.5, 4.1, 4.3, 4.7, 5.8, and 4.8 log10 colony-forming units/mL in the above microorganisms, respectively (P 〈 0.05). There was a significant increase in the amount of Cr, Zn, Fe, and Mn ions in the beer samples after PEF treatment (P 〈 0.05) leading to a statistically significant degradation in flavor and mouth feel. Further studies are needed to optimize electrode materials and PEF treatment to minimize or eliminate this degradation.

Notes: : Physicochemical parameters, sensory attributes, and total aroma emission of ‘Fuji’ apples (Malus×domestica Borkh.) were studied in relation to storage conditions, storage duration, and shelf life period. Commercially ripe fruit were analyzed after 3, 5, and 7 mo of cold storage in normal atmosphere (AIR) (210 L/m3 O2+ 0.3 L/m3 CO2) or under 3 different controlled atmosphere (CA) treatments (10 L/m3 O2+ 10 L/m3 CO2, 20 L/m3 O2+ 20 L/m3 CO2, or 10 L/m3 O2+ 30 L/m3 CO2), after which apples were kept at 20 °C for 1, 5, and 10 d. Data were subjected to partial least square regression (PLSR) analysis. Physicochemical parameters were well preserved throughout storage, especially in CA-stored apples; however, these apples showed lower total aroma emission. Sensory acceptability was also higher for CA-stored fruit after 7 mo of storage, whereas no significant differences were found for shorter storage periods. Accordingly, greater scores in sensory firmness, sensory flavor, sensory acidity, and appearance were observed for fruit stored in 10 L/m3 O2+ 10 L/m3 CO2 after long storage. Two PLSR models were established, 1 for relating physicochemical parameters to overall acceptability, and another for assessing the correlation between sensory acidity and instrumentally measured titratable acidity. The 1st PLSR model indicated that soluble solids concentration, titratable acidity, flesh firmness, and background color of the shaded side have a positive influence on acceptability. The 2nd model indicated that sensory acidity also showed an excellent correlation to instrumentally measured titratable acidity.

Notes: : The 2002 Farm Bill allows irradiated ground beef to be distributed in the Natl. School Lunch Program (NSLP). Sensory properties of irradiated ground beef specified for the NSLP were evaluated. Frozen ground beef patties with 15% fat content were either not irradiated or irradiated at doses of 1.35 and 3.0 kGy. Aroma, taste, aftertaste, and texture as well as overall liking of cooked patties after 0 and 6 mo of storage were evaluated using nontrained panels. Results showed that irradiation had no significant (P 〉 0.05) effect on the ratings of any of the sensory attributes either at 0 or after 6 mo of storage. Average ratings of liking of aroma, taste, aftertaste, and overall were higher at 6 mo than at 0 mo.

Notes: : Two studies, one in Uruguay using Uruguayan white pan bread (WPB) and another in Spain using Spanish WPB, were carried out with trained judges and consumers from the country in question. The objectives were to correlate the consumer panel acceptability, trained sensory panel scores, and instrumental measurements of defects likely to appear during WPB storage, to compare the acceptability prediction models obtained in each country, and to apply survival analysis methodology to estimate the shelf life of the product. Logistic regression was used to predict the acceptance percentage and multiple regression was used to predict acceptability. Sensory variables (strange odor) showed a greater prediction capacity for Uruguayan WPB acceptability and acceptance percentage. For the Spanish WPB, instrumental cohesiveness and moisture content variables showed a greater prediction capacity for the acceptance percentage, whereas sensory (cohesiveness and color) and moisture content variables showed a greater prediction capacity for acceptability. Uruguayan WPB shelf life was 15 d for a 50% probability of consumer rejection and 13 d for a 25% probability of consumer rejection, whereas for Spanish WPB it was 23 d for a 50% probability of consumer rejection and 16 d for a 25% probability of consumer rejection.

Notes: : Squid skin color is one of many important factors to evaluate market values. The effects of chilling and hypoxia treatments on skin color of Japanese common squid Todarodes pacificus and spear squid Loligo bleekeri were investigated to prevent highly fresh squids from abnormal fading skin color with inappropriate commercial value losses. Skin color of the squids gradually faded away during ice-cold storage. Measurements of chromato-phores expansion showed that skin color can be maintained well after death by keeping the bodies well-oxygenated and avoiding direct contact with ice.

Notes: : Lysozyme-chitosan composite films were developed for enhancing the antimicrobial properties of chitosan films. A 10% lysozyme solution was incorporated into 2% chitosan film-forming solution (FFS) at a ratio of 0%, 20%, 60%, and 100% (w lysozyme/w chitosan). Films were prepared by solvent evaporation. Lysozyme release from the film matrix, the antimicrobial activity of films against Escherichia coli and Streptococcus faecalis, and basic film properties were investigated. The lysozyme release proportionally increased with increasing initial concentration of lysozyme in the film matrix, and the amount of released lysozyme was in natural log relationship with time. The films with 60% lysozyme incorporation enhanced the inhibition efficacy of chitosan films against both S. faecalis and E. coli, where 3.8 log cycles reduction in S. faecalis and 2.7 log cycles reduction in E. coli were achieved. Water vapor permeability of the chitosan films was not affected by lysozyme incorporation, whereas the tensile strength and percent elongation values decreased with increased lysozyme concentration. Scanning electron microscopy images revealed that lysozyme was homogeneously distributed throughout the film matrix. This study demonstrated that enhanced antimicrobial activity of lysozyme-chitosan composite films can be achieved by incorporating lysozyme into chitosan, thus broadening their applications in ensuring food quality and safety.

Notes: : Color is one of the determinant sensory qualities for green tea. As cold tea beverages in clear bottles are getting more popular, the traditional evaluation methods are gradually being altered to attach more importance to the tea infusion color. For quality control, experiments were carried out on 10 green tea samples to investigate the compounds influencing the colors of dry tea leaves and infusions. By calculating the coefficients of determination between the greenness (expressed in tristimulus data) and chemical composition, chlorophylls proved to be the influential compounds for the color of dry tea leaves; water-insoluble chlorophylls were also released from the fragile tea leaves during infusion and increased both the greenness and turbidity of tea infusions. Among the flavonoids (catechins and flavonols) detected in green tea infusions, quercetin was shown to be the most important phenolic compound contributing to the greenness of tea infusion.

Notes: : The hypothesis was tested that total thermal input (combined time and temperature), rather than merely heating rate, is the determining factor in heat-induced gel formation by muscle proteins. For comminuted pastes of pollock surimi and turkey breast, rapid heating plus a brief holding time at the endpoint temperature produced similar textural properties to gels, which were heated by a slower cook schedule and cooled immediately. These results suggest that the equivalent point method, a tool used to compare and communicate equivalent heat treatments for effecting bacterial reduction and/or enzyme inactivation, can be used to identify other heat processes having similar effects on gelation.

Notes: : Nonenzymatic browning (NEB) rates of amorphous, carbohydrate-based, freeze-dried and spray-dried food model systems containing L-lysine and D-xylose as reactants (5% w/w) were investigated at different temperatures (40 °C, 50 °C, 60 °C, 70 °C, 80 °C, 90 °C). Samples were exposed to various relative vapor pressure levels (11%, 23%, and 33%) to adjust water contents. Water sorption was determined gravimetrically, and data were modeled using the Brunauer-Emmett-Teller and Guggenheim-Anderson-deBoer equations. Glass transition, Tg, was observed by differential scanning calorimetry. NEB was followed spectrophotometrically. The surface structures of freeze-dried and spray-dried models were observed by scanning electron microscopy. The freeze-dried samples and the spray-dried samples showed different surface structures and slight differences in thermal behavior. Crystallization of component sugars in the freeze-dried samples was slightly more delayed than in spray-dried samples. The glass transition temperatures in spray-dried samples were higher than those of the freeze-dried samples at the same water activity. The temperature dependence of NEB rate in both systems followed the Arrhenius kinetics, but the activation energies were different. Williams-Landel-Ferry equation could be used to model the NEB kinetics in the freeze-dried system, but for the spray-dried system, negative constant values were not within the allowable range.

Notes: : The purpose of this research was to evaluate the effects of various concentrations of glucono-δ-lactone (GDL) and skim milk powder, as well as the addition of prebiotics, on the rheology and probiotic viabilities of dairy tofu. Additionally, modern optimization techniques were applied to attempt to determine the optimal processing conditions and growth rate for the selected probiotics (Lactobacillus. acidophilus, L. casei, Bifidobacteria bifidum, and B. longum). There were 2 stages in this research to accomplish the goal. The 1st stage was to derive surface models using response surface methodology (RSM); the 2nd stage performed optimization on the models using sequential quadratic programming (SQP) techniques. The results were demonstrated to be effective. The most favorable production conditions of dairy tofu were 1% GDL, 0% peptides, 3% isomaltooligosaccharides (IMO), and 18% milk, as confirmed by subsequent verification experiments. Analysis of the sensory evaluation results revealed no significant difference between the probiotic dairy tofu and the GDL analog in terms of texture and appearance (P 〈 0.05). The viable numbers of probiotics were well above the recommended limit of 106 CFU/g for the probiotic dairy tofu throughout the tested storage period.

Notes: : Fresh ground pork patties from longissimus dorsi muscle were cooked to endpoint temperatures of 54.4 °C, 60.0 °C, 65.6 °C, 71.1 °C, 76.7 °C, or 82.2 °C. After cooking, patties were immersed in a solution of sodium nitrite (125 ppm) and water for 24 h. All patties then were heated again to a final endpoint temperature of 82.2 °C to allow possible formation of nitrosohemochrome. Instrumental color data, spectrophotometric 650/570 nm reflectance ratio, and visual panelists’ scores on both exterior and interior surfaces revealed greater redness (pinking) at the lower initial cooked temperature endpoints. Most importantly, this study showed that all patties developed pink color on reheating, regardless of initial endpoint cooked temperature.

Notes: : Lycopene, a tomato carotenoid, has been associated with the inhibition of certain chronic diseases including prostate cancer. Tomato oleoresin is a lipid-rich material resulting from successive solvent extraction of the tomato fruit. Thermal stability and isomerization of lycopene in oleoresins prepared from 3 different tomato varieties, Roma, High Lycopene, and Tangerine, and tomato peel waste, were studied at 25 °C, 50 °C, 75 °C, and 100 °C in the dark. Thermally degraded lycopene compounds and isomers of lycopene were analyzed by a combination of C30 reversed-phase high-performance liquid chromatograph with a photodiode array detector, UV-visible spectrometer, or mass spectrometer. Effects of antioxidants on lycopene were also studied at 50 °C. As the storage temperature increased from 25 °C to 100 °C, the degradation of total lycopene in oleoresin from all samples increased significantly (P 〈0.05). Lycopene at 25 °C and 50 °C may degrade mainly through oxidation without isomerization. Isomerization of lycopene in tomato oleoresins increased at 75 °C and 100 °C. Tetra-cis lycopene in Tangerine tomato varieties followed different degradation and isomerization pathways compared with all-trans lycopene in other tomato varieties. Addition of α-tocopherol or butylated hydroxytoluene slowed the rate of degradation of lycopene in oleoresin.

Notes: : The effectiveness of temperature, SO2, citric acid, and industrial juice-processing enzymes (n= 9) for producing extracts of blueberries (Vaccinium corymbosum, cv. Rubel) and blueberry skins that are rich in anthocyanins and polyphenolics were evaluated individually and/or in combination. Enzyme treatment had little effect on total monomeric anthocyanins and on total phenolics recovery. Various combinations of heat, SO2, and citric acid yielded extracts with higher concentrations of ACY and TP than the control. The distribution of anthocyanins and polyphenolics in ‘Rubel’ was also investigated. Anthocyanins existed almost exclusively in the skins, and polyphenolics were mostly in the skins with lesser amounts in flesh and seeds. Skins were also highest in antioxidant activity. All portions contained the same individual anthocyanins but in varying amounts. Cinnamic acid derivatives and flavonol-glycosides were found in the skins and seeds, whereas the flesh contained only cinnamic acids.

Notes: : The functional properties of egg white (EW) glycated with a rare D-psicose and 2 alimentary sugars (D-fructose and D-glucose) were investigated. Glycation led not only to the formation of cross-links but also to the formation of antioxidative substances to EW, the extent of which depended on the sugar used, modification, and incubation time. The temporal development of browning and fluorescence of psicose-EW was relatively faster than those of fructose-EW and glucose-EW. The psicose-EW showed the greater 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5carboxanilide (XTT) reducibility, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity, and gelling rheological properties. These results indicated that psicose-EW as a food ingredient might have the ability to improve gelling behavior and decrease oxidation reactions during food processing and storage.

Notes: : A high-performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD) method was developed to determine natural forms of soyasaponin B with (αg, βg, βa, γg, γya) and without (V, I, II, III, IV) 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) in soybean. The coefficient of variance of intra-day assays was 〈 9.51% and that of inter-day assays was 〈 10.91%. The soyasaponin I was used as external standard for quantification of all soyasaponins because it was established that their ELSD signal responses were essentially the same under the specific operating conditions. The extraction conditions were optimized and the extract storage conditions were established for analysis of soyasaponin Bs in their natural state. The energy of activation of soyasaponin Pg was 40.76 kJ/mol. The recovery rates for soyasaponin I and II were 98.3% and 93.1%, respectively. The average of total soyasaponins content from 5 soybean samples was 3.37 μmol/g with ranges from 2.78 to 4.03 μmol/g. This method is simple and easy to use, and the sample preparation takes less time than existing methods.

Notes: : Oxidative modification of low-density lipoprotein (LDL) appears to play a pivotal role in atherosclerosis. In the present study the effect of several concentrations of rutin, its aglycon quercetin, cyanidin 3-O-β-d-glucoside, its aglycon cyanidin, hydroxycinnamic acids, and a standardized red orange (Citrus sinensis varieties) extract (ROE) on LDL oxidation was tested. Lipid peroxidation was monitored by conjugated diene formation and by different electrophoretic mobility of native LDL and oxidized LDL. Results obtained in the present study suggest specific effects of flavonoids tested and, in particular, of ROE on the prevention of LDL oxidation involved in the development of cardiovascular diseases.

Notes: : An approach to classify potato chips using pattern recognition from color digital images consists of 5 steps: (1) image acquisition, (2) preprocessing, (3) segmentation, (4) feature extraction, and (5) classification. Ten chips prepared for each of the following 6 conditions were examined: 2 pretreatments (blanched and unblanched) at 3 temperatures (120°C, 150°C, and 180°C). More than 1500 features were extracted from each of the 60 images. Finally, 11 features were selected according to their classification attributes. Seven different classification cases (for example, classification of the 6 classes or distinction between blanched and unblanched samples) were analyzed using the selected features. Although samples were highly heterogeneous, using a simple classifier and a small number of features, it was possible to obtain a good performance value in all cases: classification of the 6 classes was in the confidence interval between 78% and 89% with a probability of 95%.

Notes: : The fat fraction of an oil-in-water emulsion was measured in vitro and in vivo in the gastric lumen using inversion recovery echo-planar magnetic resonance imaging and a non-exchanging, 2-compartment longitudinal relaxation model. This predicted the oil emulsion fat fraction in vitro well (Pearson's R= 0.97). Intragastric measurements in vivo correlated well with nasogastric aspirates taken from the stomachs of 7 healthy human subjects (R= 0.72). This method has potentially important applications in studying the properties of oil-in-water emulsions and also in assessing the intragastric distribution of fat emulsions in vivo and in correlating physiologic gastric emptying of fat and lipid blood absorption profiles.

Notes: : The possibility of modeling the cross-contamination of Listeria species, total Listeria monocytogenes, or specific L. monocytogenes strains using a quantitative mathematical model using Monte Carlo simulation techniques is proposed. This article illustrates this approach using 2 different models: one that tracks L. monocytogenes number and prevalence for 4 different strains (Model I) and one that tracks only prevalence for a single strain (Model II). These models have been developed to provide a starting framework for predictive modelers and scientists studying L. monocytogenes to begin research together with the ultimate goal of understanding and controlling L. monocytogenes in food-processing plants.

Notes: : The aim of the present study was to clarify by means of sensory tests whether taste, aroma, or texture is the dominant contributor to people's ability to identify the animal species of meat. The meat samples used were loins of beef, pork, and lamb; chicken thigh; and aigamo (crossbreed of domestic duck and wild duck) breast. Panelists (32 to 35 persons) wearing eye masks ate cooked pieces, patties, and heated soups prepared from the meats of these 5 species with and without pinching their noses to regulate the aroma sensation, attempted to identify the species, and gave reasons for their identifications. In the test involving meat pieces and pinched noses, the percentage of panelists identifying chicken correctly was the highest, at 72%, whereas the percentages of correct identification for all other animal species were less than 50%; however, values were higher for each species in tests without nose pinching. In the tests of meat patties involving pinched noses, the percentage of correct identification for each animal species was slightly lower in comparison with the tests of meat pieces; however, the values were higher in tests without pinched noses. In the tests of soups using pinched noses, the percentage of correct identification for each animal species was lower than 30%; however, the values were higher in tests without pinched noses. These results led us to the conclusion that aroma is the most important contributor to the identification of the animal species of meat, and texture is the 2nd most important contributor. The contribution of taste appears much smaller than that of aroma and texture.

Notes: : Consumption of omega-3 (ω-3) fatty acids is beneficial for human health. Incorporation of ω-3 fatty acids into functional foods is limited by their high susceptibility to oxidative degradation. Oil-in-water emulsions may be a more effective method to deliver ω-3 fatty acids into functional foods. Protein-stabilized oil-in-water emulsions at pH values below the isoelectric point of the protein produce cationic emulsion droplets that decrease the oxidation of lipids by decreasing iron-lipid interactions. This research showed that whey protein isolate (WPI)-stabilized algal oil emulsions at pH 3.0 had good physical and oxidative stability after pasteurization. Addition of ethylenediaminetetraacetic acid

Notes: : Boldo is an endemic plant from Chile widely used in folk medicine, whose biological (mainly antioxidant) activity has been attributed to the presence of the alkaloid boldine. The aim of this study was to determine the contribution of the flavonoid and other crude fractions from boldo leaves to the antioxidant activity of extracts. The main constituent in the flavonoid fraction was also isolated. The antioxidant activity and reducing power of crude boldo extract and fractions were measured and compared with natural and synthetic antioxidants. The antioxidant activity of boldo leaf extracts came mainly from the flavonoid fraction (44.1%) followed by the alkaloid fraction (15.6%), with catechin and boldine being the main contributors of the antioxidant activity of these 2 fractions (60.9% and 35.6% of the total activity, respectively). On the basis of these results, we propose to use catechin and boldine as markers for the antioxidant activity of boldo leaves. Tailor-made antioxidants to be used in foods can be prepared by enriching these 2 fractions.

Notes: : Seven potential variables, pretreatment temperature, pretreatment time, concentration of OH−, concentration of H+, extraction temperature, extraction time, and skin/water ratio, were investigated by a 27-3 fractional factorial design to identify critical control factors for pollock skin gelatin extraction, and 3 responses, yield, gel strength, and viscosity, were evaluated. The results suggest that 4 variables, pretreatment temperature, concentration of OH−, concentration of H+, and extraction temperature, have significant effects on gelatin extraction, and these key factors were then selected for the subsequent optimization using response surface methodology with a 4-factor, 5-level central composite rotatable design. It suggests that a concentration of OH− at 0.25 mol/L, a concentration of H+ at 0.09 mol/L, a pretreatment temperature at 2°C, and an extraction temperature at 50°C, will give the highest values for the 3 responses. The predicted responses for these extraction conditions are that the yield will be 18%, gel strength will be 460 g, and viscosity will be 6.2 c P.

Notes: : Detection of irradiated ostrich meat with doses of 1 to 10 kGy using the DNA comet assay and hydrocarbon determinations by gas chromatography-mass spectrometry was investigated. The irradiated ostrich meat showed comets with long tails whereas nonirradiated controls showed intact cells only slight comets. Concentrations of radiation-induced hydrocarbons increased with irradiation dose. Radiation-induced hydrocarbons in ostrich meat were detected at doses of 1 kGy or more; however, they were not detected in nonirradiated controls. The prominent radiation-induced hydrocarbons included 14:1, 15:0, 16:2, 16:3, 17:1, and 17:2, with 16:2 the most abundant.

Notes: : Anthocyanin pigments are responsible for the attractive red to purple to blue colors of many fruits, vegetables, flowers, and cereal grains. In plants they serve as attractants for pollination and seed dispersal, give protection against the harmful effects of UV irradiation, and provide antiviral and antimicrobial activities. Anthocyanin-based colorants are commercially manufactured for food use from horticultural crops and from processing wastes. There is a large body of knowledge on anthocyanin chemistry in the literature that includes established, systematic methods for identifying anthocyanins and measuring their concentration and color properties. Structural variation (B-ring substitution, glycosidic substitution, and acylation) produces a multiplicity (over 400) of anthocyanins. Color intensity and hue, as well as stability, are markedly affected by pH, which can be a major limitation for many food applications. Increased glycosidic substitution and acylation with cinnamic acids will improve pigment stability. However, the matrix in which the pigments exist can have a much greater impact on pigment degradation. Anthocyanin pigments are very effective scavengers of free radicals. Currently, there is much research activity on their possible health benefits. While anthocyanin-based colorants are universally approved for food use, there are considerable regulatory differences among countries.

Notes: : Structural and rheological properties have been investigated for an aerated system containing a high content of invert sugar and 2 to 10 wt% egg albumen. The specific gravity and the overrun were found to be influenced by the protein concentration and the whipping time. The systems exhibited rheopexy and shear-thinning at high shear rate, with flow data fitted to a power-law model. At small deformations, viscous character was found to dominate over elastic character over the frequency range 10−2 to 10 Hz. Images taken from confocal microscopy indicated that mean bubble size and concentration varied systematically with protein concentration and whipping time, and that more protein accumulated on the bubble surfaces as whipping proceeded. The study of this model system illustrates how the texture of a high sugar content aerated food depends on protein content and whipping conditions.

Notes: : Trials were conducted to evaluate the potential for using bacteriophages to control Salmonella in sprouting seeds. Two phages (Phage-A, capable of lysing S. Typhimurium and S. Enteritidis, and Phage-B, capable of lysing S. Montevideo) were isolated and characterized as members of the Myoviridae and Siphoviridae families, respectively. Salmonella counts increased in all inoculated seeds during soaking and mustard seeds supported greater growth of the inoculated Salmonella than broccoli seeds. A 1.37 log suppression of Salmonella growth was achieved by applying Phage-A on mustard seeds. The mixture of Phage-A and Phage-B caused a 1.50 log suppression of Salmonella growth in the soaking water of broccoli seeds. Host specificity observed in the study stresses the importance of developing phage mixtures that can control a broad range of potential contaminants.

Notes: : Ingestion of probiotics can be recommended as a preventative approach to maintaining intestinal microflora balance and thereby enhance‘well-being'. Undoubtedly, probiotic bacteria will vary in their efficacy. The literature indicates positive results in over 50 human trials with prevention/treatment of infections the most frequently reported. In theory, increased levels of probiotics may induce a ‘barrier’ influence against common pathogens. Mechanisms of effect are likely to include the excretion of acids (lactate, acetate), competition for nutrients and gut receptor sites, immuno-modulation and the formation of specific antimicrobial agents. An alternative, or additional, approach is the prebiotic concept. This takes the view that probiotics are present indigenous to the gut and that a rational approach towards increasing their numbers would be to consume food ingredients (carbohydrates) that have a selective metabolism in the lower gut. A prebiotic is‘a nondigestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon that can improve the host health.' In particular, the ingestion of fructo-oligosac-charides, galacto-oligosaccharides, and lactulose has shown to stimulate bifidobacteria in the lower gut.

Notes: : We report the development of automated flourometric immunoassay for the detection of Escherichia coli O157:H7, using antibody-directed liposomes (immunoliposomes) encapsulating fluorophore as an analytical reagent. Thiolated antibodies (anti-E. coli O157:H7) were coupled to malemide-tagged liposomes encapsulating dye. To automate the assay, a fluorescence plate reader was included in the assay system to detect fluorophore released from lysed liposomes in a microplate. The detection limit of the current assay with pure cultures of the serotype was about 104 colony-forming units (CFU)/mL. The assay can detect E. coli O157 in ground beef samples inoculated with as few as 0.8 CFU/mL after a 12-h enrichment. These results demonstrate the feasibility of using fluorophore-encapsulated immunoliposomes in a microtiter plate for the rapid and automated detection of molecules with multivalent antigenic sites.

Notes: : Flat oysters, Ostrea edulis, were emersed and stored for 23 d in freshwater ice and cold stored without ice. A control group was maintained in running seawater. Death, histological changes, bacterial growth, and changes in sensory profile were measured and described. Death of the oysters could be determined by measuring tissue pH. The sensory profiles of iced and cold-stored oysters were significantly different from the controls, and changes in sensory profile correlated with the appearance of spoilage bacteria and degradation of digestive tissues. Changes were most pronounced in iced specimens. Freshness of the oysters was correlated to the smell of sea, fresh fish, and shellfish and the appearance of the mantle.

Notes: : To construct a Cheddar taste in media similar to Cheddar cheese, we developed dairy, nondairy, and mozzarella model systems. We trained a descriptive analysis panel to evaluate real and model cheeses for a variety of taste attributes and for Cheddar-like taste. We added sodium chloride, lactic acid, citric acid, and monosodium glutamate to the model systems using mixture designs and used response surface methodology to determine optimum levels of these 3 components. Less sodium chloride and acids were needed to simulate the taste of mild Cheddar compared with aged Cheddar. None of the model cheeses closely mimicked the texture of real Cheddar. We approximated, but did not match, the taste of aged cheddar using our mozzarella base. Panelists generally rated the optimized tastes in the dairy model system as more Cheddar-like than the optimized tastes in the nondairy model.

Notes: : Eight cooked chuck muscles were subjected to Warner-Bratzler shear (WBS) force analysis in phase I of the study to choose the cooking methods and temperature combinations suitable for descriptive sensory analysis in phase II. WBS force analysis was unable to discriminate (P 〉 0.05) cooking and temperature combinations among various muscles. Nine chuck muscles were then subjected to cooking by grilling, roasting, and braising to an internal temperature of 63°C and 77°C. A panel of 10 trained judges performed the descriptive analysis of the cooked chuck muscle steaks. Grilling, under medium rare conditions, was most suitable for the chuck muscles because it yielded more juiciness and roasted flavor than any other cooking method/temperature combinations.

Notes: : Different washing treatments with chlorinated and ozonated water were applied to carrots (Daucus carota L.) on an industrial scale to improve the sensorial and microbial quality of packaged ready-to-eat produce. Quality of shredded carrots was determined by sensory evaluation and microbiological analysis. Washing shredded carrots resulted in increased sugar leaching and loss of sensorial quality, whereas prewashing uncut carrots with chlorine ensured sugar retention, reduced microbial load, and concurrently minimized cross-contamination. Comparable germ reduction was not achieved by prewashing with ozone. These findings demonstrated that prewashing uncut carrots with chlorine provided sufficient microbiological safety paired with improved sensorial properties.

Notes: : Data were collected from 902 rural and urban residents of Ohio during the winter of 2003 to assess attitudes toward the production of genetically modified (GM) plants and animals. Attitudes were assessed using Likert-type attitude statements. The theoretical perspective used in the investigation was developed from diffusion and risk perception theories. Regression modeling demonstrated that the theoretical model was very good for predicting variability in attitudes toward GM plants and animals. Approximately 61% of the variance in the dependent variable was explained by the statistical model. The findings are discussed in the context of the social acceptability of GM food and fiber products among Ohio residents.

Notes: : Modern food production technology is given great challenges by the emerging fields of biotechnology and molecular biology. Knowledge of conventional fermentation technology is upgraded by the gene level explanations of enzyme actions and physiological functions of biomaterials derived therefrom. The use of genetically modified organisms (GMOs) and their products in food widens the availability of resources while also raising public interest about safety and labeling. As an example of the application of molecular biology in conventional fermentation technology the selection of proteases from a Bacillus species grown in Korean traditional soybean fermentation starter, Meju, and the production of peptides with blood cholesterol lowering effect, obtained from soybean protein hydrolysate, are presented. Recent developments in the Korean bioindustry are reviewed as an example of the role of biotechnology in the food industry. The present status of GMO enzymes in food production is reviewed and safety issues about GMO use in the food system are discussed.

Notes: : Casein was hydrolyzed by trypsin to 5% degree of hydrolysis, and the hydrolysate was fractionated by ultrafiltration. While screening for the best emulsifying properties, the retentate fraction was systematically fractionated by ammonium sulfate precipitation, ion exchange, and reverse-phase high-performance liquid chromatography (HPLC). Residue from 30% ammonium sulfate saturation yielded 3 fractions on anion exchange chromatography. Preparative reverse-phase HPLC was used to isolate 4 peptides with good emulsifying properties for characterization. Computational methods were used to complement amino acid sequencing, and the peptides were identified as originating from αs-1 casein (f167-208), β-casein (f48-63), and (f129-184). A 4th peptide could not be conclusively identified. Properties of the emulsifying peptides are discussed.

Notes: : Mean methanethiol headspace concentrations above aqueous slurries of isolated soy proteins (ISP) increased 17-to 36-fold over the controls with the addition of L-cysteine. Corresponding hydrogen sulfide levels were also greatly increased. Dithiothreitol, sodium sulfite, and glutathione increased headspace methanethiol from aqueous ISPs 23-to 44-fold, 8-to 9-fold, and 5-fold, respectively, but did not elevate hydrogen sulfide. These observations, along with the effects from the addition of dithiothreitol/O-acetyl-serine, the addition of a pyridoxial phosphate inhibitor and the intrinsic sulfite content of ISP samples (22 to 31 ppm), indicate that methanethiol from soy proteins is formed by way of components of a sulfite-to-cysteine pathway.

Notes: Water solubility, hermal properties, tensile strength, percent elongation, oxygen permeability (OP), water-vapor permeability (WVP) of cross-linked glycerol plasticized whey protein isolate films were studied to determine the effect of cross-linkers (glutaraldehyde, formaldehyde, dialdehyde starch, carbonyldiimidazole, and UV irradiation) on film properties. With the exception of UV treatment, solubility of the films decreased (P〉0.05) upon treatment of the film-forming solutions with chemical cross-linkers. Tensile strength increased (P〉0.05), whereas percent elongation was not affected by cross-linking. Chemical cross-linking increased (P〉0.05) WVP and decreased (P〉0.05) OP of the films. UV treatment had no effect on WVP and O P. With the exception of UV-treated films, both onset temperature and degradation temperatures, as determined by differential scanning calorimetry, were increased upon cross-linking.

Notes: : The properties of total sarcoplasmic proteins from oil sardine (Sardinella longiceps)have been assessed. Ammonium sulfate fractionation of total sarcoplasmic proteins yielded 5 different fractions. The UV-ab-sorption spectra of all 5 sarcoplasmic proteins fraction showed a peak in the wavelength range of 275 to 280 nm indicating aromatic amino acid residues in its composition. The SDS-PAGE of different fractions showed components in the molecular weight range of 97 to 29 kD. The thermal stability in the temperature range of 40° to 80 °C of different fractions were varied in nature. The flow profile of different fractions indicated pseudo-plastic behavior and minimum structural impairment for fraction V at 30 and 40 °C, respectively. The gelation profile of washed sardine meat in combination with different sarcoplasmic proteins fraction revealed fraction V could enhance the storage modulus values during heating regime.

Notes: : We studied the effectiveness of partial replacement of glycerol with citric, lactic, malic, and tartaric acids on the antimicrobial activities of nisin (205 IU/g protein)-incorporated soy protein film against Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella gaminara. S. gaminara inoculated into 2.6% malic acid-incorporated films and lactic acid-incorporated films with nisin (5.7 and 3.4 log number colony-forming units (CFU)/mL, respectively) and without nisin (3.2 and 3.0 log number CFU/mL, respectively) had fewer survivors than HCl-incorporated film with and without nisin (8.6 and 7.9 log number CFU/mL, respectively). Malic acid (2.6%)-incorporated soy protein film had the fewest survivors of L. monocytogenes, S. gaminara, and E. coli O157:H7 (5.5, 3.0, and 6.8 log number CFU/mL, respectively) and has the potential to inhibit a wide spectrum of microbes in product application.

Notes: : The objective of this study was to apply mathematical expressions commonly used in the analysis of mass-transfer processes to the study of the cod desalting operation to be able to predict the changes suffered by cod during the process. The NaCl concentration in the cod liquid phase at equilibrium was well predicted by using a mass balance. It was observed that the changes in the cod liquid phase concentration and the total weight changes throughout the process could be predicted by using a pseudo-diffusional approach or a biexponential equation, whereas a linear relation was observed between the NaCl cod liquid phase concentration and the cod density.

Notes: : Low-density polyethylene film was coated with a solution containing a high-molecular-weight or low-molecular-weight methylcellulose and hydroxypropyl methylcellulose. Films contained 10000, 7500, 5000, 2500, or 0 IU/cm2 nisin. Film samples were placed into peptone water, and 10-μL samples were removed and placed onto spiral plated lawns of Listeria monocytogenes. Zones of inhibition were measured using a caliper. Films containing 5000, 7500, and 10000 IU/cm2 nisin inhibited L. monocytogenes after 30 min; films with 7500 and 10000 IU/cm2 nisin inhibited L. monocytogenes after 60 min and 8 h, respectively. No zones of inhibition were observed after 24 h and 4 d for all films. After 8 d, zones of inhibition were observed for films with all levels of nisin except 2500 IU/cm2. Films with 0 and 2500 IU/ cm2 nisin did not produce zones of inhibition throughout the study. Molecular weight of the cellulose-based carrier had no effect on inhibition of L. monocytogenes. A standard curve of inhibition was developed using solutions of 10000, 7500, 5000, 2500, or 0 IU/cm2 nisin applied directly to lawns of L. monocytogenes. The amount of inhibition using a direct application of a solution was 25% to 50% more effective for inhibition of L. monocytogenes compared with the coated film samples. Overall, the coated film samples were effective for inhibition of L. monocytogenes, particularly when 7500 and 10000 IU/cm2 nisin were used, but the release of nisin was not controlled and did not provide consistent inhibition throughout the 8-d study.

Notes: : Development of a browning inhibitor for fresh-cut apples that would not support human pathogen survival was investigated. Granny Smith and Fuji wedges were treated with acidic or neutral browning inhibitors with and without addition of sodium hexametaphosphate. Wedges in modified atmosphere packaging pouches were observed for browning during storage at 4 °C and 10 °C. A pH 2.9 dip containing ascorbic acid, citric acid, and sodium hexametaphosphate suppressed browning for at least 3 wk at 4 °C, whereas formulations without hexametaphosphate failed within 1 wk. These results demonstrate that browning in fresh-cut apples can be controlled with a formulation unlikely to support human pathogen survival or growth.

Notes: : Protein isolate (PI) was prepared from 3 cowpea varieties and 2 soybean varieties in each of 2 y. The protein contents of cowpea PI (CPI) (91.4% to 91.8%) and soy PI (SPI) (93.0% to 93.5%) were significantly different (P 〈 0.05). Electrophoretogram showed that CPI was concentrated at 60 and 40 kDa, whereas SPI showed bands at 95, 65, 60, 40, and 35 kDa. The CPI had higher lysine (49.3 to 51.8 mg/g) than SPI (41.9 to 43.4 mg/g). The CPI and SPI had surface hydrophobicities ranging from 387.8 to 572.9 and 640.6 to 643.1, respectively. Denaturation temperature (85.2 to 88.4 °C) and enthalpy (8.42 to 10.33 J/g) of CPI were similar to the 7S fraction of SPI (82.6 °C and 96.0 to 96.3 J/g). These characteristics of CPI might be useful to explain its functionalities for application in food systems.

Notes: : The effects of 1-methylcyclopropene (1-MCP) and alginate-based edible coatings on the rate of respiration, C2H4 production, color, texture, and total ascorbic-acid level of minimally processed and stored Baby Butterhead lettuce were determined. Samples were stored at 2 °C and 6 °C for 12 d. It was observed that the 1-MCP-treated lettuce had the lowest respiration and ethylene production rates. Russet spotting on the midribs of the leaves in 1-MCP-treated lettuce was the most retarded, and the total ascorbic acid level was the highest. Alginate treatment of lettuce had the most pronounced effect on crispness. Overall, the 1-MCP-treated lettuce had the best quality, followed by the alginate-coated and the control lettuce.

Notes: : We analyzed the effects of press aids on strawberry, raspberry, and blueberry juice quality and evaluated the effectiveness of dried apple pomace as an alternative press aid. Juice yields with apple pomace were similar to the yields from using rice hulls and paper. Triangle difference tests showed that there were differences between the berry juices pressed with conventional press aid to the berry juices pressed with apple pomace press aid, and paired comparison preference tests showed that the berry juices pressed with apple pomace were preferred. Flavor analysis using gas chromatography-olfactometry indicated possible off-flavors being extracted from rice hulls, such as indole and 4-vinylguaical, and from paper, such as (Z)-2-octenal and 2-nonenal, into the berry juices.

Notes: : Food entrepreneurship is a vital part of the food industry that focuses on creating specialty foods from agricultural products. Many entrepreneurial businesses are farm-based to complement the fresh market with longer shelf-life value-added processed foods that utilize products not suited for the fresh market, and excess production that commonly ends up as farm waste/losses. In some cases, the agricultural production is solely dedicated to fulfill the specialty niche market targeted by the small processor. Food entrepreneurs need comprehensive assistance to become successful processors and marketers. As start-up ventures, their knowledge and economic resources are limited. Support from university-based food venture centers must include training, counseling, technical services, regulatory compliance assistance, technology transfer, and specialized referrals. The Northeast Center for Food Entrepreneurship, a joint effort of Cornell Univ. and the Univ. of Vermont, is a successful model that benefits from key partnerships to promote food ventures in rural and urban communities. For the last 3 years the center has provided assistance and training to more than 3500 individuals interested in food entrepreneurship, and assisted the development and marketing of over 1000 specialty products. Three examples of farm-based value-added enterprises are presented to highlight the center's accomplishments and economic impact.