ALBERT

Notes: Abstract. Much of the low-lying farmland around the coastline of south-east England was once inter-tidal salt marsh, which was subsequently reclaimed from the sea and converted to farmland. It is becoming increasingly uneconomic to maintain the embankments which protect this land from the sea. ‘Managed retreat’ involves relocating the embankments further inland and recreating inter-tidal habitat in front of them. Salt marsh not only provides a protective buffer for these sea walls by dissipating wave energy, but is also important as a habitat for birds and as a source of organic matter for fish and inter-tidal fauna. When ‘managed retreat’ takes place, the creation of inter-tidal habitat occurs on soils that have undergone physical and chemical changes, some of which are irreversible. However, the indications are that rapid sedimentation creates conditions in which salt marsh plants germinate and become established; soil salinity rises quickly to a level which restricts competition from terrestrial plants, and deposited sediment is relatively rich in available phosphorus. The physical properties of the old agricultural soil influence the subsequent development of creeks.

Notes: Abstract. When the farmers of the Highlands of Tigray (northern Ethiopia) consider rock fragment cover in their fields to be excessive, they remove some of them. In addition, large amounts of rock fragments of all sizes are removed from fields for building stone bunds. Semi-structured interviews indicate that the farmers are often reluctant to take away the smaller rock fragments (i.e. 〈 5 cm across) from their fields, since they believe these benefit soil moisture conservation and protect topsoil from erosion. A field experiment was carried out on a Vertic Cambisol (average slope: 0.125 m m–1), 2 km east of Hagere Selam (subhumid climate). Rock fragments were totally, partially or not removed from the 12 runoff plots (5 m × 6 m) before the beginning of the 1999 cropping season, during which a local mixture of wheat varieties (Triticum spp.) was sown. After harvest, erosion rates were assessed by measuring deposited sediment volume in trenches at the lower side of each subplot, and grain and straw yields were assessed. We found a significant negative relationship between rock fragment cover and soil loss by water erosion. However, the resulting positive relationship between rock fragment cover and grain and straw yield was weak. This might be explained by the fact that the plot did not suffer from drought due to soil and climatic conditions. Detailed analysis showed that cover by medium and large rock fragments (〉 2 cm diameter) showed an optimum percentage cover above which crop yields decrease. A recommendation resulting from this study is to rely on the farmers’ experience: smaller rock fragments should never be removed from the surface of fields during soil and water conservation works; instead rock fragment rich soil can be used to top the stone bunds.

Notes: Abstract. The fertilizer nitrogen requirement of winter wheat was assessed in sixteen experiments on marine silt soils in Eastern England. Eight experimental sites followed potatoes (Solanum tuberosum), six vining peas (Pisum sativum) and two wheat (Triticum aestivum). The yield response to nitrogen fertilizer was much less following peas than potatoes or wheat, five sites following peas showed little response to more than 30 kg N ha–1. Previous crop explained some 79.7% of the variation in nitrogen optima. When autumn soil mineral nitrogen was also taken into account 81.9% of the variation in optimum nitrogen rate was explained (P〈0.001). The study revealed noticeably higher levels of autumn soil mineral nitrogen following vining peas on some sites than those found elsewhere in the UK and as assumed in the standard national fertilizer recommendation system.

Notes: Abstract. The aim of this study was to investigate the effects of water submergence depth on radial oxygen loss (ROL), soil solution chemistry and rice growth performance in acid sulphate soils in southern Vietnam. ROL was measured in a solution culture. In a separate pot experiment the impact of water submergence depth on rice growth and soil solution chemistry was studied. Three submergence depths were used in the two experiments (5, 10 and 15 cm). ROL declined with submergence depth and was significantly greater in young roots (with no root hairs) than in older roots. In the pot experiment rice growth and soil solution chemistry were clearly affected by the submergence depth. During the first crop at 5 cm submergence, there was a significantly higher yield and a higher oxidation state (pe+pH) compared to 10 or 15 cm submergence. The Fe concentration was significantly greater at the 5 cm depth compared to the 10 or 15 cm depth. SO42– reduction was delayed at the 5 cm depth. Rice yield was c. 25% less at the 15 cm than at the 5 cm depth. During a second crop, there was a substantial SO42- reduction and H2S formation and almost no significant effects of submergence depth on either soil solution chemistry or crop yield. In a field experiment with a dry-season rice crop, yield and Fe, Al and SO42– concentrations were higher at a shallow submergence depth than at greater depths in the same field, showing similar depth trends to those found during the first crop in the pot experiment. Farmers should be advised to use a shallow submergence depth and, if possible, avoid deep-rooted rice varieties. A conceptual model is suggested, which summarizes the relationships between ROL and soil solution chemistry.

Notes: Abstract. Tillage displaces large amounts of soil from upper slopes and deposits soil in lower landscape positions, greatly affecting productivity in these areas. The long-term effect of tillage on soil erosion was studied in four field sites growing mainly rainfed wheat. The soil loss from landscape positions with slopes, ranging from 3 to 28%, was estimated by: (a) comparing data of horizon thickness described at the same position at different times; and (b) using soil movement tracers added to the soil. Existing empirical relationships were used for estimating soil loss by tillage and runoff water, and loss in wheat biomass production. The experimental data showed soil losses of 0.4 to 1.4 cm yr–1 depending on slope gradient, plough depth, and tillage direction. In two of the sites, soil depth has been reduced by 24–30 cm in a period of 63 years. The mean soil displacement of the plough layer (30 cm thick), measured by soil movement tracers, ranged from 31 to 95 cm yr–1 depending mainly on slope gradient, corresponding to a rate of soil loss of 0.3 cm to 1.4 cm yr–1. Soil eroded from the upper slopes was deposited on the lower slopes increasing soil thickness by 0.4 cm to 1.4 cm yr–1. The application of empirical relationships, estimating soil loss by tillage and water runoff, showed that soil erosion at the field sites can be mainly attributed to tillage. The loss in wheat biomass production due to erosion was estimated at 26% on upper slopes for a period of 63 years, while a 14.5% increase in wheat production was estimated due to deposition of soil material in the lower landscape.

Notes: Abstract. An estimate of organic carbon stored in French soils to a depth of 30 cm was made using data from geo-referenced databases. We produced statistics on carbon stocks in soils according to land use, different land uses and soil type. Then, using a combination of maps of soil and land use we were able to estimate regional and national carbon stocks. This soil carbon map of France allowed us to identify the main controlling factors of the carbon distribution: land use, soil type in some cases, clay content, and elevation. Carbon stocks in French soils were found to be about 3.1 Pg (1015g).

Notes: Abstract. The problem of soil erosion is particularly evident in New Zealand, given the combination of coarse-textured soils, steep relief, high rainfall, and intensification of agriculture. A study was undertaken to assess the effects of land use change on soil erosion and sediment transport for the Ngongotaha catchment in New Zealand's North Island, using a GIS based decision support and modelling system. Model simulations considered the effect of increased catchment area under deer farming and forestry on the amount of sediment delivered to the catchment outlet, averaged over a period of six years. The simulations predicted that sediment loss from land under deer farming was considerably greater than from land under other livestock or forestry. Further model simulations testing best management practices demonstrated that sediment yield could be halved if deer farming was restricted to slopes under 20%.

Notes: Abstract. An increasing number of breeding sows is kept outdoors in Europe. Outdoor pig production has benefits in terms of animalwelfare but may have hidden costs through nutrient losses. We investigated the distribution of nutrients in sow paddocks and the consequence for losses and utilization in the succeeding crop. Significant correlation between soil inorganicNand the distance to feeding sites was observed after the paddocks had been used by lactating sows for 6 months (P〈0.01). Near to feeders inorganic N levels became extremely high whereas 30–40m from feeders some patches hadN levels in the topsoil corresponding to the levels in the reference area without sows. In the following spring only a minor part of inorganic N was still present in the top 0–40 cm. Similarly, extractable P and exchangeable K in topsoil were significantly affected by distance to feeders with the highest values close to the feeders (P〈0.001). In addition there were significant effects of the distance to huts with increasing nutrient content closer to huts. Although huge variations in dry matter production and nutrient content occurred in the succeeding potato crop, these were only weakly related to the distribution of nutrients (N, P and K) in the previous year, which explained 17%of the total variation in dry matter production. To increase nutrient efficiency in outdoor pig production a uniformdistribution of nutrients should be obtained by manipulating the excretory behaviour of the sows and stocking densities must be adjusted to locally acceptable nutrient surpluses.

Notes: Abstract. A survey of manure management practice was undertaken in 1996, by postal questionnaire submitted to a stratified sample of egg and broiler producers in England and Wales. Out of a target of 500 laying hen and 500 broiler (chickens produced for meat) production units in the survey sample, 356 (36%) returned questionnaires. The survey provided information on amount and type of manure production, manure storage and land application strategies (timing, techniques and awareness of nutrient content). Within the survey, no attempt was made to differentiate between organic and conventional production systems. About 45% of manure production was estimated to come from layer holdings, 55% from broiler litter. It was estimated that 70% of the national manure production is litter-based and about 30% are droppings collected without litter. Sawdust/shavings are the most popular bedding material, with an average final depth of 100 mm for broilers and 140 mm on litter-based layer units. Commonly, storage is available within housing for at least the length of the cropping cycle (6 weeks in broiler production, or 12 months in deep pit laying houses), around 60% of poultry manure is stored for a period following removal from the house, most commonly for 3-6 months. Overall, autumn was the peak period for manure spreading, with over 40% of laying hen manure and 50% of broiler manure applied at that time. On grassland, spreading was reasonably evenly distributed throughout the year but autumn application was favoured for arable crops, especially before the establishment of cereals and root crops, overall, almost 50% of layer and broiler manure was applied in the autumn. In the survey, up to 10% of manures were claimed to be incorporated within a day of application and about 60% within a week of application, presumably because of concern about odour nuisance. Around 25% of poultry manure was applied by contractors. A high proportion of farmers (c. 40% with layers, c. 60% with broilers) exported manures from their holdings, the proportion removed amounting to almost 90% on these farms. Although evidence elsewhere indicates that farmers make little allowance for manures in planning crop fertilizer inputs, the survey responses suggested that farmers do make an effort to allow for manures but that their confidence in the advice available to them is lacking, or they may have other technical reasons for not taking advantage of the manurial value. Information provided by the survey is of significant importance to policy makers (e.g. for the construction of environmental emissions inventories), researchers, consultants and farmers.

Notes: Abstract. The phosphorus (P) sorption and desorption dynamics of eleven major agricultural grassland soil types in Ireland were examined using laboratory techniques, so that soils vulnerable to P loss might be identified. Desorption of P from soil using the iron-oxide paper strip test (Pfeo), water extractable P (Pw) and calcium chloride extractable P (Pcacl2) depended on soil P status in all soils. However, soil types with high organic matter levels (OM), namely peat soils (%OM 〉30), had lower Pfeo and Pw but higher Pcacl2 values compared to mineral soils at similar soil test P levels. Phosphorus sorption capacity remaining (PSCr) was measured using a single addition of P to soils and used to calculate total P sorption capacities (PSCt) and degree of P saturation (DPS). Phosphorus sorption capacities correlated negatively with % OM in soils indicating that OM may inhibit P sorption from solution to soil. High organic matter soils exhibited low P sorption capacities and poor P reserves (total P, oxalate extractable P) compared to mineral soils. Low P sorption capacities (PSCt) in peat soils were attributed to OM, which blocked or eliminated sorption sites with organic acids, therefore, P remained in the soil solution phase (Pcacl2). In this work, peat and high organic matter soils exhibited P sorption and desorption characteristics which suggest that these soils may not be suitable for heavy applications of manure or fertilizer P owing to their low capacities for P sorption and storage.

Notes: Abstract. Changes in particulate organic carbon (POC) relative to total organic carbon (TOC) were measured in soils from five agronomic trial sites in New South Wales, Australia. These sites covered a wide range of different land use and management practices. POC made up 42–74% of TOC and tended to be greater under pasture and more conservative management than traditional cropping regimes. It was the form of organic carbon preferentially lost when soils under long-term pasture were brought under cultivation. It was also the dominant form of organic carbon accumulating under more conservative management practices (direct drilling, stubble retained and organic farming). Across all sites, changes in POC accounted for 81.2% (range 69–94%) of the changes in total organic carbon caused by differences in land use and management. Significant differences were found between pasture and cropped soils in the carbon content in the 〈53 μm fraction, particularly for hardsetting soils. However, even with these, POC was a more sensitive indicator of change caused by land use and management practices than TOC. The current method for measuring POC involves dispersion using sodium hexametaphosphate. The dispersing agent was found to extract 4–19 % of the TOC, leading to a significant under-estimation of POC.

Notes: Abstract. The most common way of assessing soil organic matter content is by loss on ignition, which is both simple and inexpensive. This method tends to overestimate organic matter content because additional weight losses occur during ignition. An alternative, more expensive and time-consuming method for determining soil organic matter content is by an acid dichromate oxidation. This paper compares the results of applying these methods to soil on different parent materials in two arable fields. Summary statistics and correlation coefficients showed that there were consistent relationships between the two sets of values: the stronger was for the sandy soil and the weaker was for the clay loam. This relationship can be used to improve the accuracy with which soil organic matter content is known while using fewer of the expensive measurements and more of the inexpensive ones. Two approaches to prediction were compared: the geostatistical method of cokriging, and simple linear regression. These were used to predict organic matter determined by an acid dichromate oxidation from the loss on ignition. The estimates from cokriging were more accurate but the method requires the spatial correlation to be modelled reliably. The regression results showed it to be a valuable and practical approach. Using the information from nine carefully selected sampling sites a regression line could be fitted that was representative of the full data.

Notes: Abstract. Demand for water from catchments dominated by upland peat as a source of drinking water supplies in the UK is likely to increase in the future as demand per capita continues to rise (Thomsen 1990) and/or summer droughts increase in frequency (Arnell 1992). Concern has been expressed in recent years over rising colour levels (related to dissolved organic carbon (DOC) and iron (Fe)) from such catchments (e.g. Kay et al. 1989) causing reduced drinking water quality. One of the major causes of increased DOC concentrations is rewetting following periods of relative drought (Mitchell & McDonald 1992). Experimental rewetting of a naturally drained wetland in Mid-Wales over four years was found to substantially increase the concentrations of DOC, and Fe in the pore-water, with peak values of 〉60 mg dm–3 (Fe) and 〉300 mg dm–3 (DOC) after rewetting, compared with typical values of 〈1 mgdm–3 (Fe) and 〈15 mg dm–3 (DOC) under the drained conditions. Seasonal peak concentrations of Fe and DOC have since remained at these higher levels. Rewetting produced a selective enrichment of the 〉5000 to 〈90 000 apparent molecular weight (AMW) material and this fraction was found to yield peak Fe concentrations. Two additional peaks of DOC were also found in the experimental wetland (not present in the control wetland), of 〉90 000 to 〈200 000 AMW and 〉200 000 AMW material. The AMW spectrum of DOC in the experimental wetland changed with season, and the 〉90 000 to 〈200 000 AMW fraction could only be seen in spring, representing a transient pool of carbon that is rapidly transformed in or transported from the wetland. These findings suggest that rewetting of peatland following drought (e.g. due to climate change) has the potential to reduce water quality. Moreover, recent interest in restoration (rewetting) of drained peatlands (Wheeler & Shaw 1995) could create an additional source of DOC rich water.

Notes: Abstract. The hierarchial concept of land use planning becomes less relevant in a society with continuous interactions between stakeholders, researchers, planners and politicians. In this context, land use negotiation rather than land use planning appears to be the most appropriate concept. In the negotiation process, good quality data about the land is important as land properties are, obviously, key elements to be considered. Case studies at farm and regional level have been analysed to explore answers to a number of questions. How can soil data be presented most effectively? What are the research needs? How can the large existing body of data be mobilized most effectively? Studies on regional land use in Costa Rica used methods in a logical sequence including projections, explorations and predictions of land use patterns. The work involved upscaling of data, obtained at farm level, to the regional level. Work at farm level focussed on prototyping procedures in which farming systems were ‘designed’ by close interaction between farmers and scientists, including applications of precision agriculture. Soil data demands were analysed, emphasizing the effects of using data with different degrees of detail together with the application of pedotransfer functions which effectively transform existing data into parameters that are difficult or expensive to measure directly. This not only facilitated interactions with stakeholders but also with colleague scientists in interdisciplinary teams. In addition, use of Geographical Information Systems allowed visual presentations of alternative geographical land use patterns that were associated with various scenarios, thereby facilitating the interaction processes. A plea is made to increase interaction of stakeholders and researchers by considering research programmes as vehicles for joint learning.

Notes: Abstract. Topsoil (0–15 cm) bulk density, aggregate stability, soil dispersibility, water retention and infiltration were measured between 1989 and 1996 on an Alfisol under rehabilitation in southwestern Nigeria. The planted leguminous species were Pueraria phaseoloides, Senna siamea, Leucaena leucocephala, Acacia leptocarpa and A. auriculiformis. Also, plots with natural fallow and maize/cassava intercropping were included. Level (minimum) and mound tillage with hoes was adopted for the cultivated areas under study after 4 and 6 year fallow periods. Under fallow, the soil bulk density decreased from1.56 to 1.11 t m73.The continuously cropped treatment (level tillage) had significantly higher bulk density than the fallowed subplots after 6 years. Mean soil penetrometer resistance ranged from 75 to 157 kPa for fallowed plots and from 192 to 295 kPa for the continuously cropped (level tillage) subplot. Surface soilwater contentswere similar for all the treatments during the soil strength measurements. Although soil aggregates were generally of low stability and not well formed, they were improved by fallowing.Soil structural improvement by planted fallows was similar to that by natural fallow, but the trees were more promising for long-term fallow (〉6 years) than the herbaceous P. phaseoloides. However, the improvement in soil structure after 4 or 6 year fallow could not be maintained in subsequent cropping. Furthermore, the significant improvement in soil bulk density caused by A. auriculiformis and natural fallow was more rapidly lost on the cultivated subplots compared with other fallow treatments. Thus, soil structure recovery under a fallow does not imply a sustained improvement when stress is applied to this soil. Post-fallow soil management options such as residue incorporation and tillage to ameliorate compaction or soil strength will be necessary to enhance the improvements by fallow species.

Notes: Abstract. 15N labelled NH4NO3 (fertilizer N) was applied at a rate of 50 kg N ha–1 to an Ando-Humic Nitisol and two maize crops grown on it. About 20 months later, soil cores were taken to a depth of 2.5 m. Leached fertilizer N was found between 1.4 m and 1.8 m deep and was delayed relative to net drainage by between 4.2 and 4.9 pore volumes. Anion exchange capacity (AEC) increased ten-fold down the profile, up to 2.9 cmolckg–1. The delay to fertilizer N leaching was predicted to be between 4.1 and 5.3 pore volumes when calculated from the AEC and from an equation relating delay due to AEC in laboratory columns of repacked soil obtained by Wong et al. (1990b). It was concluded that the nitrate leaching delay equation was also valid in undisturbed field profiles. Two concentration maxima for mineral N were found, which did not usually coincide with the fertilizer N and were thought to result from mineralization of soil organic matter and plant residues at the end of each season. The delay equation overestimated their leaching delay but the results were considered close enough to support the hypothesis for their formation.

Notes: Abstract. Regions in the Po Valley, Northern Italy, are characterized by intensive crop-livestock farming systems. A simulation model has been chosen for an inter-regional project, which should help in defining groundwater vulnerability and pollution risk on a regional scale, in relation to agricultural land use, by allowing the prediction of nitrate leaching under different climate, soil, crop and management scenarios. The model derives from the coupling of a hydrological model, MACRO, simulating water flow and solute transport in structured soils, with a model simulating soil N dynamics, SOILN. The aim of this work was to test the model's ability to simulate nitrate leaching through soil after land spreading of pig slurries. A dataset obtained from lysimeter experiments which had been carried out in the period 1976–1981 was used for this purpose. Four soil types were compared (silty clay, sandy loam, loam and sand) in factorial combination with four rates of pig slurry (0, 142, 284, 426 g of N m–2, accumulated values from 1976 to 1979) for a seven crop sequence. The efficiency of the MACRO model ranged from 0.96, in the sandy-loam soil, to 0.81, in the sand. Percolation was usually under-estimated, the relative error ranging from 0.7 to 14.6, depending on the soil. The low efficiency of the SOILN model in simulating nitrate leaching is attributed to the lack of knowledge of the mechanisms regulating N transformation processes and especially the mineralization of pig slurry N. This lack of knowledge hampers the correct setting of the N transformation parameter values. A remarkable improvement of the model's performance was obtained by changing a few coefficients which control the mineralization-immobilization turnover of the faeces-organic N. The model efficiency, following this recalibration, ranged from –0.62 to 0.84, and the relative error ranged from –56 to 35, depending on soil and treatment. N leaching was under-estimated at the low pig slurry N application rates and over-estimated at the high ones.

Notes: Abstract. The effectiveness of contour grass strips in erosion control was investigated in a field experiment involving two grass treatments (Festuca ovina and Poa pratensis) and a bare soil control on an erodible sandy loam soil on a 5° slope using simulated rainstorms of 40 mm h–1 for 45 minutes duration. The grass strips resulted in significantly (P 〈 0.05) lower runoff and soil loss than the bare soil but there was no significant difference in the performance of the two grasses, despite their differences in density, height and leaf size. The effect of the lower density of the Poa pratensis was offset by its larger stem diameter so that the surface area facing the flow was similar for both grasses. Instead of acting as a filter with sedimentation occurring within the barrier, the grass strips operated by ponding water upslope of the barriers. Deposition then occurred in the ponded area above the barrier.

Notes: Abstract. Since the 1980s, land use in rural areas of China has changed greatly as the result of political initiatives. These changes have caused soil nutrient changes which are examined in this paper for Zunhua County, northern China from 1980 to 1999. The areas of farmland, grassland, and paddy decreased greatly and were replaced by increases in forest and residential land. The soils under forest in 1999 transformed from farmland in 1980 increased in organic matter by 21%, total nitrogen by 18%, available nitrogen by 65%, available phosphorus by 17% and available potassium by 17%. Similarly, in the area which was converted from farmland in 1980 to grassland in 1999, soil organic matter, total nitrogen, available nitrogen, available phosphorus, and available potassium increased by 38%, 37%, 71%, 2% and 28%, respectively. Changes from farmland to forest and grassland not only changed land cover but also improved soil fertility and probably reduced soil nutrient losses.

Notes: Abstract. Soil management studies show that intensive arable agriculture can lead to a decline in both organic matter levels and the stability of the soil structure. It is a priority to understand how soil structure responds when fresh organic materials are added to poor quality degraded arable soils. This is of particular interest because of its implication for carbon sequestration. We investigate whether the addition of organic materials can form stable aggregates in a degraded soil. Grass or peat residues were added to samples of soil obtained from the continuous grassland and arable plots of the long-term experiment at highfield, IACR-Rothamsted (UK) and incubated at 2° and 24°C, for upto 8 weeks at -5 kPa. At 1 day and at 2, 4 and 8 weeks the soil was slaked in de-ionised water and the aggregate size distributions were measured. The data was used to calculate mean weight diameters (MWD). The treatments with added grass showed increased aggregated relative to the control; the treatments with added peat did not. At 24°C the value of MWD increased with the incubation period, but at 2°C there was no further aggregation beyond week two. Respiration measurements were made and the samples that released the most CO2 were also those which re-aggregated the most. This suggests that the process of aggregation is microbiologically mediated. The results are discussed within the broader context of the implications of soil organic matter content on soil management.

Notes: Abstract. By comparing field measurements from 1989, 1997, and 1998, the differences between farmland (sloping farmland, sloping farmland with contour cultivation, terraced farmland) and orchard (terraced orchard, unterraced orchard), in the Three Gorges Reservoir Area, were significant for runoff (P〈0.01), erosion (P〈0.05) and nutrient loss (P〈0.05). Taking into account economic costs and environmental influences, reasonable and sustainable land use on slopes of 25° in the Three Gorges Reservoir Area should be unterraced orchard.

Notes: Abstract. Paper sludge composted or stored in static piles for six months was compared to raw and lime-stabilized sludge in a three-year pot experiment (loam) and a four-year field experiment (silt loam) at rates of up to 40 t DM ha-l. The original sludge contained equal amounts of fibre sludge and biological sludge, mixed with bark in 1:1 ratio to improve the structure. The N content in composted sludge (1.30%) was markedly lower than that of the uncomposted piles (1.98%), indicating significant loss during composting. The yield results were generally inversely proportionate to the C:N ratios of the sludges applied. Sludge from the uncomposted piles gave significant grain yield increases in the year following sludge application, while the other sludge types gave variable results. In the residual years there was, generally, a small but positive effect on yield from all the sludge types. The N and P content in grain generally increased with sludge application, but only the higher rates gave statistically significant increases. Sludge application also increased the Zn content in grain, while Mn, B and Cu was less affected. The increase in Cd content was very small. The 40 t ha-l sludge rate tended to increase the residual mineral N in soil at the field site and thus the risk of nitrate leaching.

Notes: Abstract. Measures to reduce ammonia (NH3) emissions by incorporating livestock manures into the soil may increase the potential for nitrate (NO3-) leaching. The Manure Evaluation Routine (MANNER) model estimates the amount of N available to crops following livestock manure applications after calculating losses due to NH3 volatilization and NO3- leaching. The main objective of this study was to use the MANNER model to quantify the impact on NO3- leaching of introducing measures to reduce NH3 emissions, following application of livestock manures. The data produced were also used to make preliminary estimates of the likely effect of selected NH3 abatement techniques on the potential for nitrous oxide (N2O) emissions. At typical UK rates of application, the potential for increased NO3- leaching following either injection of slurry or rapid incorporation of solid manures was greatest for broiler/turkey manure (22–58 kg N ha–1) and least for straw-based cattle manure (6–10 kg N ha–1). The results suggest that in order to avoid substantially increasing the potential for NO3- leaching as a consequence of NH3 abatement, livestock manures should not be applied by low NH3 emission techniques prior to autumn-sown crops in the UK. Instead, low-emission applications should be made from October onwards to grassland and where possible, late autumn-sown combinable crops or to arable land which will be planted in the spring. However, in several areas of England and Wales there is currently insufficient land planted to spring crops on which to incorporate the livestock manures produced in those areas.

Notes: Abstract. A survey of cattle manure management was undertaken in England and Wales, in 1997, by postal questionnaire sent out to a stratified sample (by unit size) of 1750 dairy and 1750 beef producers. The level of response obtained, with 471 dairy farmers (27%) and 515 beef farmers (29%) returning questionnaires, reflects well on the interest shown by the industry and on the survey design. The survey provided information on manure production and storage, when and how applied and nutrient value. Dairy farms are estimated to produce manures in the form of c. 65% slurry and 35% farmyard manures (FYM) and, beef units, 80%FYM and 20%slurry (based on survey response data, animal numbers and calculations of undiluted outputs of excreta). Slurry storage within both dairy and beef systems is typically up to 3–6 months capacity, although there is no storage for an estimated 16% of dairy and 25% of beef slurry. Autumn and winter spreading is common practice, with 40–50% of slurry and 50–60% of FYM applied at that time. Although some evidence suggests that farmers make little allowance for the nutrient content of manures in planning fertilizer inputs, the results of this survey suggest that many farmers do make some effort to utilize manure nutrients. However, they currently fail to be assured by the advice available to them or they lack confidence in manures as nutrient sources for a number of technical reasons. Information provided by the survey may be important to policy makers, researchers and consultants, as well as farmers.

Notes: Abstract. In 1983, an annual Survey of Fertiliser Practice in England and Wales was extended to Scotland, to provide comprehensive information on inorganic fertilizer, lime and also organic manure use in mainland Britain. It was based on an annual sample of about 1500 farms, selected from the Agricultural Census and stratified by farm type and size. Results from the first fifteen years (1983–97) show that fertilizer nitrogen (N) rates on both tillage crops and grassland peaked at 157 and 132 kg ha–1, respectively, in the mid 1980s and subsequently decreased by c.10%. The majority of N was applied in straight form (without P or K) to tillage crops and in compound form (containing two or more nutrients e.g. NPK; NK) to grassland. Total N use on cereals showed little change but autumn-applied N decreased on both winter cereals and winter oilseed rape. Total N rates decreased on oilseed rape and, to a smaller extent, on maincrop potatoes and sugarbeet. Between 1983–87 and 1993–97, mean phosphate (P2O5) rates declined by almost 10% on both tillage crops (from 58 to 53 kg ha–1) and on grassland (from 25 to 23kg ha–1). The corresponding mean potash (K2O) rates decreased slightly on both tillage crops (from 64 to 62 kg ha–1), and on grassland (from 32 to 31 kg ha–1), although annual usage was more variable on grassland. Sulphur use increased appreciably on cereal and oilseed rape crops between 1993, when S data were first recorded in the survey, and 1997 when 13% and 30%, respectively, of these crop areas received S-fertilizer. However, on grassland, S use remained very low. Average lime use increased on both tillage crops and grassland between the mid 1980s and mid 1990s, from 10 to 12% and 4 to 7% of the total area, respectively. The proportion of land receiving organic manures remained at c. 16% for tillage cropping but increased slightly for grassland, from a mean of 40% in 1983–87 to 44% in 1993–97. Manures were applied throughout the year but about half the applications to tillage land, and a quarter of those to grassland, were made in autumn when the risk of subsequent nitrate leaching loss is greatest.

Notes: Abstract. A two year field experiment was carried out in a semiarid Mediterranean area in order to evaluate, the effect on soil erosion of adding different urban organic wastes: a stabilized municipal waste (compost), an unstabilized municipal waste, and an aerobic sewage sludge. All the treatments significantly reduced soil erosion, compared to the control soil. The soil amended with compost was the most effective treatment, reducing soil loss by 94% and runoff by 54%.

Notes: Abstract. Cashew soils of South Eastern Tanzania become acidified due to sulphur used for controlling powdery mildew disease (Oidium anacardii Noack). The buffering capacity of surface and subsurface horizons of 35 soil profiles of major cashew growing areas –- the Makonde plateau, its piedmont and inland plains –- was studied. The buffering capacity of surface and subsurface horizons was strongly correlated with clay content and weakly with organic carbon content. In addition, it was only weakly correlated with total exchangeable bases and available P of the surface horizon, but strongly with soil pH, base saturation and cation exchange capacity of the clay fraction of the subsurface horizon. Highly weathered sandy soils, dominant on the Makonde plateau and common on the Piedmont, had the lowest buffering capacity. Soils from the inland plains had better buffering capacities as they are generally more clayey or are less weathered. The risk of severe acidification and of a decline in productivity of cashew and of food crops is highest on the Makonde plateau. Further development and dissemination of methods which can reduce the use of sulphur are required.

Notes: Abstract. Biomass productivity and soil microbial responses to long-term CO2 enrichment have been investigated in a Mediterranean natural forest ecosystem. Several biochemical parameters have been measured on soil samples taken from six open top chambers (OTCs), enclosing clumps of natural Mediterranean woody vegetation including: Quercus ilex L., Phillyrea angustifolia L., Pistacia lentiscus L. and Myrtus communis L. The CO2 concentration of the air inside the OTCs was either ambient or ambient plus 350 μmol mol–1 (c. 710 ppm as mean daily value). Microbial C biomass, microbial respiration, dehydrogenase, β-glucosidase, acid phosphatase and protease activities, inorganic N and soluble P, were tested in order to evaluate soil microbial size and activity. Statistically correlated seasonal patterns have been identified in some biochemical parameters in response to climatic conditions, soil nutritional status and the physiology of the vegetative cover. In situ soil respiration and above- and below-ground productivity were also measured. Microbial responses to CO2 enrichment were observed only at the beginning of the study and a general progressive reduction of the CO2 effect was recorded as monitoring continued. These results are in agreement with data from literature regarding similar studies on natural complex communities.

Notes: Abstract. The Century model was used for estimating soil carbon levels under grassland at the regional scale in the Pampean Region of Argentina. Predicted values were compared with observed soil carbon contents obtained from soil surveys and the differences considered to be the results of cropping on soil organic matter. The Pampean Region was divided into five major sub-regions and carbon in the top 20 cm of each estimated by Century using aggregated soil and climatic data. In four of the sub-regions small differences between predicted and observed carbon contents were obtained which suggested little land use effect on soils. In the Rolling Pampa, a northern portion of the Pampean Region, observed carbon content was about half of Century prediction. In this sub-region, the main agricultural area of the country, cropping intensity, rainfall and temperature are higher than in the rest of the Pampas. A degradation index constructed by a multiplicative approach, taking into account percentage of surface cropped, rainfall and temperature was 2–5 times higher in the Rolling Pampa than in the other pampean sub-region. The difference between predicted C and observed C in the Rolling Pampa was attributed to carbon losses by cropping in a warm and wet climate.

Notes: Abstract. Recent developments in in situγ ray spectrometry offer a new approach to measuring the activity of radionuclides such as 137Cs and 40K in soils, and thus estimating erosion or deposition rates and field moist bulk density (ρm). Such estimates would be rapid and involve minimal site disturbance, especially important where archaeological remains are present. This paper presents the results of a pilot investigation of an eroded field in Scotland in which a portable hyper pure germanium (HPGe) detector was used to measure γ ray spectra in situ. The gamma (γ) photon flux observed at the soil surface is a function of the 137Cs inventory, its depth distribution characteristics and ρm. A coefficient, QCs, derived from the forward scattering of 137Cs γ ray photons within the soil profile relative to the 137Cs full energy peak (662 keV), was used to correct the in situ calibration for changes in the 137Cs vertical distribution in the ploughed field, a function of tillage, soil accumulation and ρm. Based on only 8 measurements, the agreement between in situγ ray spectrometry and soil sample measurements of 137Cs inventories improved from a non significant r2=0.05 to a significant r2=0.62 (P〈0.05). Erosion and deposition rates calculated from the corrected in situ137Cs measurements had a similarly good agreement with those calculated from soil cores. Mean soil bulk density was also calculated using a separate coefficient, QK, derived from the forward scattering γ photons from 40K within the soil relative to the 40K full energy peak (1460 keV). Again there was good agreement with soil core measurements (r2=0.64; P〈0.05). The precision of the in situ137Cs measurement was limited by the precision with which QCs can be estimated, a function of the low 137Cs deposition levels associated with the weapons testing fallout and relatively low detector efficiency (35%). In contrast, the precision of the in situ ρm determination was only limited by the spatial variability associated with soil sampling.

Notes: Books Reviewed in this Article: Fertiliser recommendations for agricultural and horticultural crops (RB209) Published with the permission of MAFF by HMSO, 7th edition 2000.

Notes: Abstract. Field peas (Pisum sativum L.) were grown in sequence with winter wheat (Triticum aestivum L.) or spring barley (Hordeum vulgare L.) in large outdoor lysimeters. The pea crop was harvested either in a green immature state or at physiological maturity and residues returned to the lysimeters after pea harvest. After harvest of the pea crop in 1993, pea crop residues (pods and straw) were replaced with corresponding amounts of 15N-labelled pea residues grown in an adjacent field plot. Reference lysimeters grew sequences of cereals (spring barley/spring barley and spring barley/winter wheat) with the straw removed. Leaching and crop offtake of 15N and total N were measured for the following two years. These treatments were tested on two soils: a coarse sand and a sandy loam. Nitrate concentrations were greatest in percolate from lysimeters with immature peas. Peas harvested at maturity also raised the nitrate concentrations above those recorded for continuous cereal growing. The cumulative nitrate loss was 9–12 g NO3-N m–2 after immature peas and 5–7 g NO3-N m–2 after mature peas. Autumn sown winter wheat did not significantly reduce leaching losses after field peas compared with spring sown barley. 15N derived from above-ground pea residues accounted for 18–25% of the total nitrate leaching losses after immature peas and 12–17% after mature peas. When compared with leaching losses from the cereals, the extra leaching loss of N from roots and rhizodeposits of mature peas were estimated to be similar to losses of 15N from the above-ground pea residues. Only winter wheat yield on the coarse sand was increased by a previous crop of peas compared to wheat following barley. Differences between barley grown after peas and after barley were not statistically significant. 15N lost by leaching in the first winter after incorporation accounted for 11–19% of 15N applied in immature pea residues and 10–15% of 15N in mature residues. Another 2–5% were lost in the second winter. The 15N recovery in the two crops succeeding the peas was 3–6% in the first crop and 1–3% in the second crop. The winter wheat did not significantly improve the utilization of 15N from the pea residues compared with spring barley.

Notes: Abstract. In view of the increasing wheel loads of agricultural vehicles, the question arises as to whether soil can recover from the mechanical impact of traffic. The damage to soil quality depends also on the soils resilience. This paper presents a new approach to monitoring vertical soil movement in situ. We assessed the effects of trafficking the soil with excavators and sugarbeet harvesters by monitoring surface and subsurface levels. The caterpillar loads of the crawlers varied from 13 to 19 t, the wheel loads of the sugarbeet harvesters from 6 to 11 t. Classical geodetic levelling was used to record soil surface movement and a hydrostatic settlement meter measured deformation at three depths within the soil profile. The results of three field tests prove the importance of wheel load and soil moisture for soil compaction. Trafficking very dry soil with an excavator did not cause significant plastic deformation in 30 cm depth. Conversely, trafficking wet soil with a sugarbeet harvester led to soil sinkage of 1 to 2 cm even at 60 cm depth. Increased wheel load in subsequent passes led to greater subsidence than during the first pass. Settlement decreased from the soil surface to deeper layers, but it remained throughout the monitoring period of up to 12 days. No soil recovery from plastic deformation was recorded within this time. The measuring system has the potential for long-term monitoring of the mechanical recovery of the soil. Additionally, it can contribute to the validation of mechanical impact models, which are based on soil stresses.

Notes: Abstract. Two case studies on Danish sandy loams investigated the long-term effects of fertilization and crop rotation. Case Study 1 compared a diversely cropped organically farmed soil (DFG(1)) with a conventionally farmed soil predominantly growing annual crops (DFA), both receiving animal manure. In Case Study 2, a diversely cropped organically managed soil (DFG(2)) receiving animal manure was compared with an almost exclusively cereal cropped conventionally farmed soil receiving no animal manure (CCC). A multi-level experimental strategy was followed to compare integrating field methods with specialized laboratory methods. Ease of tillage was described in the field and characterized using a drop shatter field test and by measuring aggregate tensile strength. Fitness as a seedbed was characterised visually in the field and from the drop shatter test. Impedance to seedling emergence and root penetration was evaluated by measuring bulk soil shear strength. For Case Study 1, soil tilth was better for the DFA soil than for the DFG(1) soil (i.e. lower soil strength and higher ease of fragmentation and friability index for DFA). However, a crumbly structure and a moderate bulk density suggested that the DFG(1) soil was also a favourable medium for plant growth. For Case Study 2, the CCC soil had a lower organic matter content, higher bulk density, higher soil strength, and lower ease of fragmentation in comparison with the DFG(2) soil.

Notes: Abstract. A two year field study was conducted to evaluate the effects of straw management and tillage on the soil profile (1.5m) water storage, nature of the moisture profile, infiltration and sorptivity as influenced by rainfall, evaporativity (E0) and soil texture. The straw mulch treatment stored more moisture under low E0 rainy conditions in three coarse to medium textured soils. Straw incorporation treatment was better under low E0 rain free conditions, as well as under high E0 rainy conditions in the two coarser textured soils. In the coarsest textured soil, tillage and straw mulching were not effective in maintaining greater soil water storage under high E0 because of the very open nature of the soil. The soil moisture profiles showed a sharper increase in water content below the tilled layer in the tillage and straw- incorporation treatments than the untreated and straw mulch treatments. Tillage and straw incorporation treatments increased the sorptivity of the soil compared with untreated and straw mulch treatments respectively. The results of this study suggest that when selecting a suitable soil water conservation practice to increase water storage in the soil profile, information on soil texture and weather (rain and evaporativity) must be considered.

Notes: Abstract. Phosphorus (P) use in UK agriculture is reviewed and a P balance sheet presented. The productive grassland and arable area has accumulated an average P surplus of c. 1000 kg ha–1 over the last 65 years. Over the period 1935–1970, the annual P surplus more than doubled due to an increase in animal numbers and associated requirements for inorganic fertilizers and livestock feeds. Since 1970, surplus P has declined by c. 40% as crop yields and P offtake have continued to increase while fertilizer and manure P inputs have remained relatively constant. In 1993, P use efficiency (P imports/P exports) in UK agriculture was estimated at 25% leading to an average annual surplus of 15 kg P ha–1 yr–1, although the latter has since decreased slightly due to reduced fertilizer use. Intensification and specialization of agriculture has also increased the range in P surpluses that are likely between livestock and arable dominated systems. The largest P surpluses occur in the relatively limited areas of arable soils which receive manure from intensive pig and poultry units, whilst farms without manure inputs generate only small surpluses, or are in balance. The cumulative P surplus has led to a build-up of soil total and easily-exchangeable P, especially in areas receiving both fertilizers and manures. Fundamental differences in P use efficiency, surplus P accumulation and the potential for P loss to water, exist between arable and grassland farms and it is important to separate these, due to the marked regionalization of UK agriculture. More judicial use of feeds and fertilizers is required to further reduce the P surplus and minimize the long-term risk of water eutrophication.

Notes: Abstract. The ability of two nitrogen cycle models, of contrasting complexity, to predict N mineralization from a range of grassland soils in the UK, was evaluated. These were NCYCLE, a simple mass balance model of the N cycle in UK grasslands, and CENTURY, a more complex model simulating long-term C, N, P & S dynamics in grassland ecosystems. The models were tested using field measurements of net N mineralization from a range of grassland soils (differing in soil type, history & management practice), obtained over a 2 year period using a soil core incubation technique. This method was considered to measure the total net release of mineral N from the soil organic matter over a specified time, including N which may have been recycled several times. NCYCLE consistently under-estimated mineralization rates at all sites. By contrast, there was some correlation between CENTURY predictions of net N mineralization and field measurements. This may have reflected the different abilities of the two models to simulate N recycling. Neither model, however, was able to predict adequately the effect of cultivation and reseeding on net N mineralization.

Notes: Abstract. Nitrate leaching and soil mineral N status under grassland were measured on three contrasting soils, spanning winters 1995/96, 1996/97 and 1997/98, in Western England. The soils investigated were a freely draining silty clay loam (Rosemaund), a well drained loam (IGER 1) and a poorly drained clay loam (IGER 2). The effects of reseeding (ploughing and resowing grass) at IGER 1 and IGER 2 in autumn 1995 or 1996 were compared with undisturbed pasture. Reseeding at Rosemaund, in autumns 1995 or 1996, or spring 1996 was compared with undisturbed pasture of 3 sward ages (2, 5, 〉50 years).Nitrate-N leaching losses during the winter immediately following autumn reseeding ranged between 60 and 350 kg N ha–1 in 1995/96, depending on soil type, sward management history and rainfall. Losses were much less in the following winter when treatments were repeated (10–107 kg N ha–1).Reseeding in spring had little effect on soil mineral N content or leaching losses in the following autumn, compared with undisturbed pasture. Similarly, leaching losses from autumn reseeds in the second winter after cultivation were the same as undisturbed pasture (1-19 kg N ha–1). The effect of ploughing grassland for reseeding was relatively short-term, in contrast to the effect of repeated annual cultivation associated with arable rotations.

Notes: Abstract. Intensively managed grasslands are potentially a large source of N2O in the North Coast of Spain because of the large N input, the wet soil conditions and mild temperatures. To quantify the effect of fertilizer type and management practices carried out by farmers in this area, field N2O losses were measured over a year using the closed chamber technique. Plots received two types of fertilizer: cattle slurry (536 kg N ha–1) and calcium ammonium nitrate (140 kg N ha–1). N2O losses were less in the slurry treatment than after mineral fertilizer. This was probably due to high, short-lived peaks of N2O encountered immediately following mineral N addition. In contrast, the seasonal distribution of N2O losses from the slurry amended plot was more uniform over the year. The greater N2O losses in the mineral treatment might have been enhanced by the combined effect of mineral fertilizer and past organic residues present from previous organic amendments. Weak relationships were found between N2O emission rates and soil nitrate, soil ammonium, soil water content and temperature. Better relationships were obtained in the mineral treatment than in the slurry plots, because of the wider range in soil mineral N. Water filled pore space (WFPS) was a key factor controlling N2O emissions. In the 〉 90% WFPS range no relationships were found. The best regressions were found for the mineral treatment in the 40–65% WFPS range, 49% of the variance being explained by soil nitrate and ammonium content. In the 65–90% WFPS range, 43% of the variance was explained by nitrate only, but the inclusion of soil ammonium did not improve the model as it did in the 40–65% WFPS range. This fact indicates that nitrification is likely to be an important process involved in N2O emissions at the 40–65% WFPS.

Notes: Abstract. Boreholes have been constructed at eight sites on the Permo-Triassic Sandstone and Chalk aquifers to assess the extent of chemical and microbiological contamination emanating from unlined farm manure stores. Slurry along fracture faces in the Chalk was found on cores taken from beneath two stores. Porewaters from the Chalk sites and one of the Sandstone sites were discoloured and showed high concentrations of nitrate, ammonium and organic carbon to depths in excess of 10 m. Although Cryptosporidia and Escherichia coli O157 were found in many of the cattle slurry lagoons, neither were found in the aquifer material beneath. The self-sealing of unlined slurry stores is seen as a crucial step in minimizing leakage. A simple mass balance shows farm boreholes near to contaminant sources are at greater risk than public supply wells. Contaminant modelling shows discontinuing use of an unlined farm manure store will lead to little difference in solute concentrations over the short to medium term. Groundwater is most at risk where the water table is shallow since direct hydraulic connection between the lagoon base and the water table considerably increases the rate of vertical migration. This is of greatest significance for pathogens that are thought to be relatively short lived in the subsurface. Under the majority of situations minimal threat is posed to potable groundwater drinking supplies.

Notes: . Cryptosporidium meleagridis is a common cause of cryptosporidiosis in birds. In addition, recent reports have described the parasite as an etiologic agent of cryptosporidiosis in both immunocompetent and immunocompromised humans. Therefore, it is important to genetically characterize isolates of C. meleagridis from different hosts and geographic areas, and to develop molecular tools to differentiate isolates from various hosts or areas. In this study, a total of 11 isolates of Cryptosporidium meleagridis from both human and avian hosts were examined at three genetic loci: the small-subunit rRNA, 60-kDa glycoprotein precursor, and 70-kDa heat shock protein genes. Two genotypes of C. meleagridis were seen at the small-subunit rRNA locus. These differed from each other by the presence or lack of a heterogeneous copy of the gene and an ATT repeat. The 60-kDa glycoprotein precursor gene divided these eleven isolates of C. meleagridis into six genotypes with high sequence diversity between groups. The highest genetic heterogeneity, however, was seen at the 70-kDa heat shock protein locus, and was primarily present at the 3′end of the gene. This heterogeneity separated eight isolates of C. meleagridis into six genotypes. These data could be useful in the development of molecular tools to promote understanding of the transmission of C. meleagridisiin humans.

Notes: Cell culture infectivity assays can provide an accurate means of detecting viable Cryptosporidium parvum oocysts from environmental samples or to test the effects of various treatments on oocyst infectivity. Cell culture assays can also be used to test candidate chemotherapeutic agents. The use of a human cell line provides a situation close to human infection. The present assay uses an anti-Cryptospordium primary antibody, combined with a biotinylated secondary antibody, and an immunoperoxidase detection system. Cryptosporidium parvum oocysts excysted in vitro when placed on monolayers of HCT-8 cells and developmental stages including schizonts and merozoites were visualized using light microscopy of the immunoperoxidase stained slides and by transmission electron microscopy of infected HCT-8 cell cultures. Because the immunoperoxidase system used gives a permanent preparation, the cell cultures can be retained and examined later. Dose titration of oocysts indicated that as few as 50 inoculated oocysts could be detected. The activity of paromomycin was evaluated in this system and 500 μg/ml produced a 97.8% reduction in infection.

Notes: . We applied a two-dimensional pulsed-field gel electrophoresis procedure to the genomes of two karyotype variants assigned to two different strains of the microsporidian Encephalitozoon cuniculi, termed D (strain III) and F (strain II). Data obtained for BssHII and Mlul restriction fragment length polymorphisms in each chromosome are compiled and compared to the reference strain I variant A. Six Insertion/Deletion (InDels) are found in subterminal position, some of these being characteristic of either D or F. Like in strain I, the terminal fragments extending between each telomere and rDNA locus are conserved in length for each chromosome. They are however smaller than in reference variant. This size reduction is estimated to be 2.5 kbp for the strain III isolate and 3.5 kbp for the strain II isolate. We hypothesize that for the three E. cuniculi strains, all chromosome extremities are prone to a constant process of sequence homogenization through mitolic recombination between conserved regions.

Notes: . The single name Pneumocystis carinii consists of an heterogeneous group of specific fungal organisms that colonize a very wide range of mammalian hosts. In the present study, mitochondrial large subunit (mtLSU) and small subunit (mtSSU) rRNA sequences of P. carinii organisms from 24 different mammalian species were compared. The mammals were included in six major groups: Primates (12 species). Rodents (5 species). Carnivores (3 species). Bats (1 species), Lagomorphs (1 species), Marsupials (1 species) and Ungulates (1 species). Direct sequencing of PCR products demonstrated that specific mtSSU and mtLSU rRNA Pneumocystis sequence could be attributed to each mammalian species. No animal harbored P. carinii f. sp. hominis. Comparison of combined mtLSU and mtSSU aligned sequences confirmed cospeciation of P. carinii and corresponding mammalian hosts. P. carinii organisms isolated from mammals of the same zoological group systematically clustered together. Within each cluster, the genetic divergence between P. carinii organisms varied in terms of the phylogenetic divergence existing among the corresponding host species. However, the relative position of P. carinii groups (rodent, carnivore or primate-derived P. carinii) could not be clearly determined. Further resolution will require the integration of additional sequence data.

Notes: SUMMARY. Toxoplasma gondii infections have been reported in a number of marine mammals. Presently it is not known how these animals acquire T. gondii from their aquatic environment. The eastern oyster, Crassostrea virginica, has been shown to remove Cryptosporidium oocysts from seawater and a similar phenomenon may be occurring with T. gondii oocysts and marine invertebrates. The present study was done to determine if eastern oysters could remove and retain T. gondii oocysts from seawater. Oocysts of the VEG strain of T. gondii (1 × 106 oocysts) were placed in seawater (32 ppt NaCl) containing live eastern oysters. The infected seawater was removed one day postinoculation (PI) and replaced with fresh seawater. Selected oysters were removed at 1, 3 and 6 days PL Hemolymph, gill washes, and oyster tissue were collected separately at each observation time. The oyster tissue was homogenized and all 3 samples fed separately to mice. Toxoplasma gondii positive mice were observed at each time period. The results indicate that T. gondii oocysts can be removed from seawater by eastern oysters and retain their infectivity. Contaminated raw oysters may serve as a source of T. gondii infection for marine mammals and humans.

Notes: . Eleven clones of a single strain of Leishmania infantum (MCAN/ES/88/ISS441, Doba) were analyzed for biological behavior in vivo and in vitro. Different clones showed differences in growth dependent upon the two culture media employed. All clones displayed only slight differences in H2O2 and NaNO2 sensitivity compared to the original strain, whereas in vitro infectivity for mouse peritoneal macrophages differed significantly among the clones. In vivo infections in hamsters correlated strongly with in vitro infectivity. The phenotypic differences found suggest a polyclonal structure for the Leishmania infantum strain studied.

Notes: . A new myxosporean, Leptotheca sparidarum n. sp., is described from the trunk kidney of two sparid fish, Sparus aurata and Dentex dentex, in several culture facilities from the Western Mediterranean coasts. It is distinguished from all the previously described species by spore morphometrics. Spores with two equal polar capsules and one binucleated sporoplasm. Spore measurements from D. dentex were 5–7.1 μ long × 8.8–12.3 μ thick × 5.88–6.18 μ wide; polar capsules 2.6–3.5 μ in diam. Spore measurements from S. aurata were 5.1–8.24 μ long × 9.41–11.76 μ thick; polar capsules 2.4–3.2 μ in diam. No significant differences were detected between either host. Prevalence could reach 21.4% in D. dentex and 19% in some stock of S. aurata. Renal tubules were the typical site of infection, which was also found in ureters and glomeruli, but seldom in the epiepithelial position of the gut. Spores were formed in disporous sporoblasts, and spore maturation seemed to proceed from the host epithelium towards the lumen. Trophozoites were attached to the host epithelial cells of renal tubules by pseudopodial-like projections, which were inserted into gaps between epithelial cells. Also, cell junctions were observed between primary cells and between neighboring spores.

Notes: . We have isolated and analysed an α-tubulin-encoding gene (atub1) in an early-diverging eukaryote, Trichomonas vaginalis. The complete atub1 open reading frame included 1,356 bp encoding a polypeptide of 452 amino-acyl residues. A second α- tubulin gene (atub2) was amplified by PCR using primers derived from consensus α-tubulin amino acid sequences. Both T. vaginalisα-tubulin sequences showed high identity to those described in other parabasalids (94.4%–97.3%), and exhibited a high degree of similarity to sequences from Metazoa (such as pig brain) and diplomonads (such as Giardia). Despite large evolutionary distances previously observed between trichomonads and mammals, the three-dimensional model of the T. vaginalis tubulin dimer was very similar to that of pig brain. Possible correlations between α-tubulin sequences and posttranslational modifications (PTMs) were examined. Our observations corroborated previous data obtained in T. vaginalis using specific anti-PTMs antibodies. As described in the related species Tritrichomonas mobilensis, microtubules are likely acetylated, non-tyrosinated, glutamylated, and non-glycylated in T. vaginalis. Evolutionary considerations concerning the time of appearance of these tubulin PTMs are also discussed since trichomonads are potentially one of the earliest diverging eukaryotic lineages.

Notes: . A new species of naked amoeba, Platyamoeba pseudovannellida n.sp., is described on the basis of light microscopic and fine structural features. The amoeba was isolated from the Salton Sea, California, from water at a salinity of ca. 44‰. Locomotive amoebae occasionally had a spatulate outline and floating cells had radiating pseudopodia, sometimes with pointed tips. Both these features are reminiscent of the genus Vannella. However, the surface coat (glycocalyx) as revealed by TEM indicates that this is a species of Platyamoeba. Although salinity was not used as a diagnostic feature, this species was found to have remarkable tolerance to fluctuating salinity levels, even when changes were rapid. Amoebae survived over the range 0‰ to 150%c salt and grew within the range 0%c to 138%c salt. The generation time of cells averaged 29 h and was not markedly affected by salt concentration. This is longer than expected for an amoeba of this size and suggests a high energetic cost of coping with salinity changes. The morphology of cells changed with increasing salinity: at 0‰ cells were flattened and active and at the other extreme (138‰) amoebae were wrinkled and domed and cell movement was very slow. At the ultrastructural level, the cytoplasm of cells grown at high salinity (98‰) was considerably denser than that of cells reared at 0‰.

Notes: . Naegleria fowleri, an amoeboflagellate, is the causative agent of Primary Amoebic Meningoencephalitis, a fulminating disease of the central nervous system. In order to elucidate the mechanisms of pathogenicity of this amoeba, a cDNA expression library was prepared from N. fowleri RNA. A specific protein was found to be expressed from a cDNA clone designated Mp2CL5. Northern blot analysis showed that the Mp2CL5 mRNA was expressed in pathogenic N. fowleri but was not expressed in non-pathogenic Naegleria species nor in Acanthamoeba. Western blot analysis using anti-. N fowleri antiserum demonstrated that IPTG-induced Escherichia coli Mp2CL5 expressed a 23-kDa recombinant protein. The Mp2CL5 recombinant protein was histidine-tagged and purified to homogeneity from E. coli. A polyclonal rabbit antiserum was prepared against the purified Mp2CL5 recombinant protein. This antibody was used to further characterize the Mp2CL5 native protein expressed by Nfowleri. Western blot analysis in conjunction with immunofLuorescence microscopy demonstrated the presence of a native protein of 17 kDa on the plasma membrane of N. fowleri trophozoites. The native N. fowleri protein was expressed in the logarithmic phase of trophozoite growth and the production of this protein increased through the stationary phase of growth. Studies are in progress to examine further its role as a virulence factor.

Notes: . Homopolar doublets with twofold rotational symmetry were generated in Paramecium tetraurelia and in P. undecaurelia by electrofusion or by arrested conjugation. These doublets underwent a complex cortical reorganization over time, which led to their reversion to singlets. This reorganization involved a reduction in number of ciliary rows, a progressive inactivation and loss of one oral meridian, and a reduction and eventual disappearance of one cortical surface (semicell) situated between the two oral meridians. The intermediate steps of this reorganization included some processes that resemble those previously described in regulating doublets of other ciliates, and others that are peculiar to members of the “P. aurelia” species-group and some of its close relatives. The former included a disappearance of one cortical landmark (a contractile vacuole meridian) and transient appearance of another (a third cytoproct) within the narrower semicell. The latter included a reorganization of the paratene zone and the associated invariant (non-duplicating) region to occupy the entire narrower semicell and a redistribution of zones of most active basal-body proliferation within the opposite, wider semicell. The final steps of reorganization involved anterior displacement, invagination, and resorption of one of the two oral apparatuses and eventual disappearance of the associated oral meridian. An oral meridian deprived of its oral apparatus, either by spontaneous resorption or microsurgical removal, could persist for some time in “incomplete doublets” before regulating to the singlet condition. The phylogenetically widespread events encountered in the regulation of doublets to singlets suggest that Paramecium shares some of the global regulatory properties that are likely to be ancestral in ciliates. The more specific events are probably associated with the complex cytoskeletal architecture of this organism and with the frequent occurrence of autogamy that was described in the preceding study (Prajer et al. 1999).

Notes: . We employed an improved fixation procedure for electron microscopy using ruthenium red, and found a bundle of contractile tubules inside the axopodia of the heliozoon Actinophrys sol. Upon food uptake, the tubules shorten and transform into a mass of small granules when rapid axopodial contraction occurs, suggesting that these structures are involved in the process of axopodial contraction. The relationship between transformation of the contractile tubules and accompanying disassembly of the axonemal microtubules was studied by examining the ultrastructure of the contractile tubules after disassembly of the microtubules was artificially induced by cold or colchicine treatment. Granulation of the contractile tubules was induced by cold but not by colchicine treatment. During recovery from cold treatment, granular forms of the contractile tubules became re-elongated and their initial tubular appearance was restored. These results suggest that the contractile tubules in heliozoon axopodia play a role in repetitive cytoplasmic contraction.

Notes: . Directed migration of trophozoites from the midgut toward the Malpighian tubules is essential for Ascogregarina taiwanensis (Apicomplexa: Lecudinidae) to complete its developmental cycle within the natural host Aedes albopictus. We have obtained a 275-bp actin cDNA fragment amplified from extracted mRNAs of migrating trophozoites, suggesting the involvement of actin in trophozoite motility. Down-regulation on the migration of the trophozoite was seen in mosquito larvae fed with cytochalasin D, ML-7. and BDM, indicating that myosin, in the form of an actomyosin system, may also be involved in driving motility of the trophozoite. The “protruding apparatus” (PA) formed at the anterior end of trophozoites during the migrating stage had significant deposits of actin by immunofluorescent microscopy. Moreover, PA formation was enhanced in response to elevated levels of 20-hydroxyecdysone (20-HE) in cultures of alimentary canals in which the trophozite was contained. Thus, 20-HE may also promote expression of actin and perhaps myosin simultaneously.

Notes: . A quantitative colorimetric in situ hybridization assay was developed for detecting Cryptosporidium parvum infection in cell cultures using a digoxigenin-labeled probe targeting 18S rRNA. Intra-cellular developmental stages of C. parvum such as trophozoites and meronts were clearly discerned by light microscopy as localized areas of dark purple/black precipitate against a colorless background. Infections developed focally and the term infectious focus was applied to each cluster of developmental stages. There were no significant differences in the number of infectious foci following 24 h or 48 h incubation. However, 24 h and 48 h dose response curves were significantly different when infectivity was measured as the number of developmental stages per monolayer, with an average of 5.3-fold more stages following 48 h incubation. When infectivity was expressed as the number of infectious foci per inoculum oocyst converted to a percentage, it was demonstrated that the rate of infection decreased with increasing oocyst age. Oocysts of the Iowa isolate that were 7–10 days old demonstrated 7.8 ± 2.4% infectivity (mean ± standard deviation) compared to 4.2 ± 0.8% for 21–28 day-old oocysts and 1.4 ± 1.3% for 42–70 day-old oocysts. The assay also detected infection with other genotype 2 oocysts and a genoptye 1 isolate. This assay provides a direct quantitative approach for measuring C. parvum infectivity in cell culture.

Notes: . The ciliate Sorogena stoianovitchae, which can form a multicellular fruiting body, has been classified based upon its ultrastructure and morphology: the oral and somatic infraciliature of S. stoianovitchae most closely resemble those of members of the order Cyrtolophosidida in the class Colpodea. We characterized the small subunit ribosomal DNA (SSU rDNA) gene sequence from S. stoianovitchae and compared this sequence with those from representatives of all ciliate classes. These analyses placed S. stoianovitchae as either sister to members of the class Nassophorea or Colpodea. In an in-group analysis, including all SSU rDNA sequences from members of the classes Nassophorea and Colpodea and representatives of appropriate outgroups, S. stoianovitchae was always sister to Platyophrya vorax (class Colpodea, order Cyrtolophosidida). However, our analyses failed to support the monophyly ot the class Colpodea. Instead, our data suggest that there are essentially three unresolved clades: (1) the class Nassophorea; (2) Bresslaua vorax, Colpoda inflata, Pseudoplatyophrya nana, and Bursaria truncatella (class Colpodea); and (3) P. vorax and S. stoianovitchae (class Colpodea).

Notes: . Germinal-vesicle-breakdown (GVBD) was induced if a 132,000-g supernatant of Tetrahymena thermophila homogenates was injected into Xenopus oocytes. Using this induction of GVBD as a bioassay system, a GVBD-inducing substance was purified from the Tetrahymena by ultra-filtration, liquid chromatography, and electroelution from a band on native-PAGE gel. Proteins eluted from the single band on the native-PAGE gel induced GVBD in the absence of oocyte protein synthesis. This band resolved into two bands on SDS-PAGE: 60 and 1 12 kDa. The 60 kDa protein was the active fraction inducing GVBD. Immunoprecipitation of the 60 kDa protein prevented the GVBD-inducing activity, supporting the conclusion that the 60 kDa protein is the GVBD-inducing substance. An immunoblot with anti-60 kDa monoclonal antibody and PSTAIR antibody showed that p 13sue1 -beads could remove cdc2 homologues from T. thermophila supernatant but could not remove the GVBD-inducing activity. The 60-kDa protein appeared at the same time as micronuclear division and disappeared at the beginning of the macronuclear division during synchronous cell division. The cyclic appearance of the 60-kDa protein in the T. thermophila cell cycle suggests that this protein has a cell cycle function. Key Words. cdc2 homologue, cell cycle, M-phase promoting factor, PSTAIR antibody, p13sue1-beads, Xenopus oocytes.