Publication Date:
1998-03-21
Description:
Mice homozygous for a disrupted allele of the mismatch repair gene Pms2 have a mutator phenotype. When this allele is crossed into quasi-monoclonal (QM) mice, which have a very limited B cell repertoire, homozygotes have fewer somatic mutations at the immunoglobulin heavy chain and lambda chain loci than do heterozygotes or wild-type QM mice. That is, mismatch repair seems to contribute to somatic hypermutation rather than stifling it. It is suggested that at immunoglobulin loci in hypermutable B cells, mismatched base pairs are "corrected" according to the newly synthesized DNA strand, thereby fixing incipient mutations instead of eliminating them.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cascalho, M -- Wong, J -- Steinberg, C -- Wabl, M -- 1R01 GM37699/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1998 Feb 20;279(5354):1207-10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0670, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9469811" target="_blank"〉PubMed〈/a〉
Keywords:
*Adenosine Triphosphatases
;
Alleles
;
Amino Acid Sequence
;
Animals
;
B-Lymphocytes/immunology
;
Base Composition
;
Base Sequence
;
Cloning, Molecular
;
Crosses, Genetic
;
*DNA Repair
;
*DNA Repair Enzymes
;
*DNA-Binding Proteins
;
Female
;
Gene Rearrangement
;
*Genes, Immunoglobulin
;
Heterozygote
;
Immunoglobulin Heavy Chains/chemistry/genetics
;
Immunoglobulin Variable Region/chemistry/*genetics
;
Immunoglobulin lambda-Chains/chemistry/genetics
;
Male
;
Mice
;
Mice, Knockout
;
Molecular Sequence Data
;
*Mutation
;
Proteins/*genetics/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink