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  • Springer  (92)
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  • 1
    ISSN: 1570-7458
    Keywords: host suitability ; acceptance ; biological control ; new associations ; Lepidoptera ; Pyralidae ; New World ; Old World ; stemborers ; Braconidae ; larval parasitoids ; Gramineae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present study examined the acceptability and suitability of Old World stemborers (Chilo partellus and C. orichalcociliellus) for the development of New World parasitoids (Apanteles deplanatus and A. minator) and New World stemborers (Diatraea saccharalis and D. grandiosella) for the development of Old World parasitoids (Cotesia sesamiae, C. flavipes and C. chilonis). Results revealed that acceptance and suitability were high in old associations. In new associations, parasitoids accepted about 60% of the new association hosts. In addition, 10 out of 17 new associations were successful. Apanteles species appeared to be more physiologically host specific than Cotesia species. For example, two of four new association hosts were accepted by A. deplanatus and only one (D. saccharalis) was partially suitable for progeny development. Among the Cotesia species, Cotesia flavipes appeared to have a wider host range than the two other species. It attacked all hosts offered and successfully parasitized all but one (D. grandiosella). Diatraea saccharalis was accepted and was a suitable host for the development of all parasitoid species tested, whereas D. grandiosella was unsuitable for the development of four out of five parasitoid species tested. No clear pattern was observed as behavioral acceptance did not always agree with the pattern of physiological suitability. Implications of these findings for importation biological control of stemborers are discussed.
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  • 2
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    Entomologia experimentalis et applicata 91 (1999), S. 327-336 
    ISSN: 1570-7458
    Keywords: biological control ; in vitro rearing ; rearing techniques ; host selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An artificial host egg was developed as a first attempt to improve the techniques for in vitro rearing of Trichogramma galloi Zucchi and T. pretiosum Riley (Hymenoptera, Trichogrammatidae). Plastic membranes (polyethylene, polypropylene and polystyrene) of different thickness were tested for their usefulness in manufacturing artificial eggs, using a thermal system to produce semi-spherical cupules on the plastic surface. These artificial eggs were filled with larval hemolymph of Helicoverpa zea (Boddie) (Lepidoptera, Noctuidae), and the optimal size of the artificial egg and density of females/artificial egg for the highest host acceptance (percentage of artificial eggs with at least one parasitoid egg) as well as the ideal level of parasitism (eggs/artificial egg) were determined. High-density polyethylene 7–8 and 9–10 μm thick resulted in the best acceptance and level of parasitization for both parasitoid species. Six females per artificial egg resulted in the best rate of parasitization for these parasitoids, although these data were not different when using 4 or 5 females of T. galloi. The size, shape and surface texture of the artificial eggs were found to provide enough cues to elicit parasitization behavior in Trichogramma females, since no chemical stimulus was provided.
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  • 3
    ISSN: 1570-7458
    Keywords: Trichogramma spp. ; Helicoverpa armigera ; Chrysoperla carnea ; Portugal ; parasitism ; biological control ; non-target species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Parasitism of two host species by five Trichogramma species (Hymenoptera, Trichogrammatidae) was studied in the laboratory. The host species were: i) the bollworm Helicoverpa armigera Hübner (Lepidoptera, Noctuidae), an important pest of many crops in the tropics and subtropics, and ii) one of its natural enemies, the lacewing Chrysoperla carnea Stephens (Neuroptera, Chrysopidae), a predator often used as a biological control agent. The proportion of H. armigera eggs parasitized from the total number of parasitized hosts differed between Trichogramma species. The average number of parasitized eggs per female in 24 h by Trichogramma pintoi and T. bourarachae was 10 of H. armigera and about 0.5 of C. carnea. For the other three Trichogramma species (T. cordubensis, T. evanescens and T. turkestanica) these averages varied from 6 to 11 H. armigera eggs and from 3 to 4 C. carnea eggs. Total adult offspring production, contacts with hosts, secondary clutch size and sex-ratio of each Trichogramma species were determined as well. The results show that sympatric Trichogramma may parasitize target and non-target species in different proportions. If this difference corresponds to the field situation, simple laboratory tests could be performed to select not only efficient biogical control agents, but also species which are the least detrimental to non-target hosts.
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  • 4
    ISSN: 1570-7458
    Keywords: host feeding ; host paralysis ; oviposition ; pre-oviposition phase ; Hypothenemus hampei ; Scolytidae ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of the density of the coffee berry borer, Hypothenemus hampei (Coleoptera: Scolytidae) on reproductive and host-feeding behaviours of the parasitoid Cephalonomia stephanoderis (Hymenoptera: Bethylidae) was evaluated under laboratory conditions. The number of hosts used for oviposition was density-dependent at low host density. Beyond a density of six hosts/day, the oviposition rate reached a maximum of 1.2 eggs/day due to egg limitation. Cephalonomia stephanoderis females responded to increasing host availability with a linear increase in host feeding. Overall, parasitoids killed more coffee berry borers by feeding and paralysis than by parasitism. At low host density, the pre-oviposition phase was extended, oogenesis was delayed, more males were produced, and host feeding occasionally occurred concurrently with oviposition. We suggest that the efficacy of C. stephanoderis as a biological control agent depends on seasonal variations in host density. Inoculative vs inundative releases in coffee plantations are discussed in relation to the abundance of the coffee berry borer during the fructification and interharvest periods.
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  • 5
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    Entomologia experimentalis et applicata 91 (1999), S. 449-457 
    ISSN: 1570-7458
    Keywords: Schizaphis graminum ; Aphelinus asychis ; aphid ; parasitoid ; natural enemy ; biological control ; greenbug ; host selection ; plant volatile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aphelinus asychis (Walker) can be valuable as a biocontrol agent of the aphid, Schizaphis graminum (Rondani), a major pest on grain crops in the United States. This study reports the wind tunnel, and olfactometric responses of this parasitoid to the host (aphid), plant (sorghum), and host-plant complex (aphid-infested sorghum). In addition, the parasitoids' response to malathion-treated plants is also presented. The goal of the present study was to test the hypothesis that volatiles associated with the host attract natural enemies, as reported in cases of many hymenopterous parasitoids, and also to learn about the effects of insecticides on these parasitoids. In wind tunnel studies, these parasitoids moved upwind in the direction of the host-infested plant. There was no direct flight observed, however, these parasitoids were observed to hop and jump, and sometimes walk to their host. In the olfactometer experiments, we found that A. asychis is attracted to host-plant complex. The parasitoids' response to malathion in the olfactometer suggested that a malathion formulation when applied to plants can lure these beneficials, thus providing new insight into the ongoing task of integrating chemical and biological control of insect pests.
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  • 6
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    Entomologia experimentalis et applicata 93 (1999), S. 267-273 
    ISSN: 1570-7458
    Keywords: Macrolophus caliginosus ; mirid bug ; life table ; longevity ; fecundity ; development time ; mortality ; sex ratio ; oviposition period ; intrinsic rate of increase ; spider mite ; Tetranychus urticae ; tomato ; biological control ; glasshouse pests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The life table characteristics of the polyphagous mirid Macrolophus caliginosus Wagner (Heteroptera: Miridae) preying on various stages of Tetranychus urticae Koch (Acari: Tetranychidae) with tomato as host plant were described at 22 °C. The following average parameters were obtained: Female longevity: 28.7 days; fecundity: 0.7 eggs/female/day; egg mortality: 2.6%; pre-oviposition period: 5.5 days; oviposition period: 18.1 days; post-oviposition period: 3.2 days; juvenile development time: 26.8 days; juvenile mortality: 34.9%; and sex ratio (♀/(♀+♂): 0.46. Life table parameters were estimated as net reproduction rate (R 0): 6.15; intrinsic rate of increase (r m): 0.031 day−1; finite rate of increase (λ): 1.032; mean generation time (T c): 58.17 days; and doubling time (T 2) 22.2 days. The parameters obtained were in accordance with those reported for M. caliginosus fed on another mite species (T. turkestani Ugarov & Nikolski (Acari: Tetranychidae)). However, compared to the performance of M. caliginosus fed on common glasshouse insect pests, a diet consisting of only mites appeared to be inferior. However, being a voracious predator, M. caliginosus may be a valuable addition to existing methods of mite control.
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  • 7
    ISSN: 1570-7458
    Keywords: biological control ; food web interactions ; volatiles ; Phytoseiidae ; predatory bugs ; thrips ; predator-predator interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Arthropods use odours associated with the presence of their food, enemies and competitors when searching for patches. Responses to these odours therefore determine the spatial distribution of animals, and are decisive for the occurrence and strength of interactions among species. Therefore, a logical first step in studying food web interactions is the analysis of behaviour of individuals that are searching for patches of food. We followed this approach when studying interactions in an artificial food web occurring on greenhouse cucumber in the Netherlands. In an earlier paper we found that one of the predators of the food web, the predatory mite Phytoseiulus persimilis Athias-Henriot, used to control spider mites, discriminates between odours from plants with spider mites, Tetranychus urticae Koch, and plants with spider mites plus conspecific predators. The odours used for discrimination are produced by adult prey in response to the presence of predators, and probably serve as an alarm pheromone to warn related spider mites. Other predator species may also trigger production of this alarm pheromone, which P. persimilis could use in turn to avoid plants with heterospecific predators. We therefore studied the response of the latter to odours from plants with spider mites and 3 other predator species, i.e. the generalist predatory bug Orius laevigatus (Fieber), the polyphagous thrips Frankliniella occidentalis and the spider-mite predator Neoseiulus californicus (McGregor). Both olfactometer and greenhouse release experiments yielded no evidence that P. persimilis avoids plants with any of the 3 heterospecific predators. This suggests that these predators do not elicit production of alarm pheromones in spider mites, and we argue that this is caused by a lack of coevolutionary history. The consequences of the lack of avoidance of heterospecific predators for interactions in food webs and biological control are discussed.
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  • 8
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    Entomologia experimentalis et applicata 93 (1999), S. 51-61 
    ISSN: 1570-7458
    Keywords: Metarhizium anisopliae ; Atta sexdens rubropilosa ; leaf-cutting ants ; biological control ; social homeostasis ; entomopathogenic fungus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There is growing interest in the use of entomopathogenic organisms to control leaf-cutting ants (Hymenoptera: Formicidae: Attini). However, the way leaf-cutting ants react as a colony to biohazards is poorly understood. We investigated the effects of Metarhizium anisopliae (Metschnikoff) (Deuteromycotina: Hyphomycetes) applied to the foraging arenas of mini-nests (queenless sub-colonies) of the leaf-cutting ant Atta sexdens rubropilosa (Forel). Dry spores were applied either alone or mixed with citrus powder, at 0.5 g or 0.05 g per mini-nest. The spores were removed four days after application, and all dead ants removed every three days. Ant numbers near the Metarhizium increased as the ants attempted to clean up the biohazard. The ants attempted to place the spores in piles, which they then covered over with other material. They were able to deal with the low doses in this way, but the high doses overwhelmed them. All treated mini-nests suffered increased ant mortality during the first ten days after application. This mortality was particularly high in the media worker caste which had played the major role in attempting to clean up the spores. Foraging activity decreased, as did the health of the fungus gardens. The mini-nests exposed to the low dose of spores mixed with citrus powder then recovered fully. The health of the other treated mini-nests declined gradually until around 26 days after application, when they began deteriorating sharply. However, the decline of these mini-nests after day 26 was not due directly to the pathogenic action of the Metarhizium, nor to the initial ant mortality it had caused. The results suggest that the social stress caused by even such a short-lived Metarhizium epizootic was sufficient to cause the decline and ultimate death of the mini-nests. This has important implications for the control of leaf-cutting ants. It also demonstrates how important the social homeostasis of the colony is to leaf-cutting ants.
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  • 9
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    Entomologia experimentalis et applicata 90 (1999), S. 103-107 
    ISSN: 1570-7458
    Keywords: learning ; Orius majusculus (Reuter) ; Heteroptera ; Anthocoridae ; predator ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 10
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    Entomologia experimentalis et applicata 90 (1999), S. 183-189 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Aphelinidae ; Platygasteridae ; Hemiptera ; Aleyrodidae ; whiteflies ; Bemisia ; Amitus bennetti ; parasitoid ; biological control ; life history ; instar preference ; oviposition behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amitus bennetti Viggiani & Evans (Hymenoptera: Platygasteridae) is a recently described parasitoid of the silverleaf whitefly, Bemisia argentifolii Bellows & Perring (Homoptera: Aleyrodidae). Behaviour and life history of the parasitoid are described. The first nymphal instar of B. argentifolii is preferred by the parasitoid, but the 1st through 4th instar may be parasitised. Females first investigate hosts with their antennae, then walk over the host, and eventually step with their front legs on the leaf and insert their ovipositor inside the host facing away from the host, while the hind legs are still on the host. The time from encounter to oviposition (=latency to oviposition) is shortest on the 1st instar. Oviposition duration (mean=39 s) comprises 50% of the handling time. Development time from egg to adult decreases from 72 days at 15 °C to 42 days at 20 °C to 28 days at 25 °C. We estimate that 400 degree days is required for development, with a development threshold of 10 °C. Adult longevity in the absence of hosts was 29, 26 and 19 days and with hosts present 8, 8 and 5 days at 15, 20 and 25 °C, respectively. Amitus bennetti is proovigenic and oviposits most eggs shortly after adult emergence. During the first day of their adult lives females laid 1, 31 and 49 eggs at 15, 20 and 25 °C, respectively. Compared with other parasitoid species, the development time of A. bennetti is very long, and the implications of this for management of B. argentifolii are discussed.
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  • 11
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    Entomologia experimentalis et applicata 93 (1999), S. 77-86 
    ISSN: 1570-7458
    Keywords: Acari ; behaviour ; biological control ; Gerbera jamesonii ; herbivore induced synomones ; infochemicals ; indirect defence ; Phytoseiidae ; semiochemicals ; terpenoids ; Tetranychus urticae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Jasmonic acid (JA) is a plant hormone that is involved in the induction of plant defence in response to herbivore attack. We studied the effect of exposure of gerbera leaves to JA on indirect plant defence, i.e. attraction of natural enemies of herbivores. Treatment of gerbera leaves with JA or feeding damage by the herbivorous spider mite Tetranychus urticae, both induced the production of a complex odour blend that attracts the predatory mite Phytoseiulus persimilis. This phytoseiid predator is a very effective biological control agent of the spider mite T. urticae. Comparison of headspace composition of gerbera leaves exposed to either JA or T. urticae revealed a large degree of resemblance, but some quantitative and qualitative differences were recorded. The major chemical group in both treatments is formed by the terpenoids which quantitatively comprised up to 80% of the total odour blend. These terpenoids included (E)-4,8-dimethyl-1,3,7-nonatriene, (E)-β-ocimene and linalool that are known to attract P. persimilis. Aldehydes, alcohols, esters and ketones, together with nitrogenous compounds formed the remaining constituents of the odour blend. The induction of predator attractants in plants by JA may be applied in biological control programs, which is discussed in this paper.
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  • 12
    ISSN: 1572-9397
    Keywords: evolutionary algorithms ; genetic algorithms ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper shows how evolutionary algorithms can be described in a concise, yet comprehensive and accurate way. A classification scheme is introduced and presented in a tabular form called TEA (Table of Evolutionary Algorithms). It distinguishes between different classes of evolutionary algorithms (e.g., genetic algorithms, ant systems) by enumerating the fundamental ingredients of each of these algorithms. At the end, possible uses of the TEA are illustrated on classical evolutionary algorithms.
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  • 13
    ISSN: 1573-0832
    Keywords: biological control ; compatible solutes ; Epicoccum nigrum ; polyols ; solid substrate fermentation ; water activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Epicoccum nigrum conidia were produced by solid fermentation on wheat grains (cv. Rendeveaux and Brigadier) at different water activities (aw). Conidial production was highest at high aw(0.996) than at reduced aw (0.98). However, conidial production at reduced aw was improved when the aw of the substrate was adjusted with a mixture of glycerol and water. Maximum levels ofconidiation were 7–11 × 106 conidia g−1 grain. The aw of the solid substrate affected the pattern of accumulation of compatible solutes in the conidia. Mannitol was the main polyol in all conidialtypes. However, the amounts of mannitol were higher in conidia produced at high aw. At reduced aw the conidia of E. nigrum accumulated moreglycerol, which is more efficient in the osmorregulation proccess than mannitol. Arabitol accumulated in low amounts, specifically in conidia produced at the lower aw, on cv. Rendeveaux but not on cv. Brigadier. Trehalose was detected in higher amounts in cv. Rendeveaux than in cv. Brigadier, andthe amounts were higher in conidia produced at high aw. A significant amount of endogenous solutes was detected in the washing liquid used for the separation of the conidia.
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  • 14
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    Integrated pest management reviews 4 (1999), S. 127-143 
    ISSN: 1572-9745
    Keywords: wheat ; stored-grain ; integrated pest management ; aeration ; biological control ; grain sampling ; insect monitoring ; modeling ; area-wide IPM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Management of stored-grain insect pests by farmers or elevator managers should be based upon a knowledge of the grain storage environment and the ecology of insect pests. Grain storage facilities and practices, geographical location, government policies, and marketing demands for grain quality are discussed as factors influencing stored-grain insect pest management decisions in the United States. Typical practices include a small number of grain samples designed to provide grain quality information for segregation, blending and marketing. This low sampling rate results in subjective evaluation and inconsistent penalties for insect-related quality factors. Information on the efficacy of insect pest management practices in the United States, mainly for farm-stored wheat, is discussed, and stored-grain integrated pest management (IPM) is compared to field-crop IPM. The transition from traditional stored-grain insect pest control to IPM will require greater emphasis on sampling to estimate insect densities, the development of sound economic thresholds and decision-making strategies, more selective use of pesticides, and greater use of nonchemical methods such as aeration. New developments in insect monitoring, predictive computer models, grain cooling by aeration, biological control, and fumigation are reviewed, their potential for improving insect pest management is discussed, and future research needs are examined.
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  • 15
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    Integrated pest management reviews 4 (1999), S. 21-37 
    ISSN: 1572-9745
    Keywords: carrot weevil ; IPM ; Anaphes spp. ; biological control ; ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The carrot weevil, Listronotus oregonensis (LeConte) (Coleoptera: Curculionidae) is a major pest of carrots in northeastern North America. The females oviposit in the leaf petiole and the developing larvae make tunnels in the carrot roots. Exclusive reliance on chemical control and the poor control obtained, prompted, in the early 1980s, research on the ecology and physiology of the pest and its natural enemies. Carrot weevil females start their oviposition by 147±9 DD7°C but they oviposit only in carrots past the 4 true-leaf-stage. As a result, oviposition is delayed, and damage reduced, in late-sown carrots. Monitoring carrot weevil adults in the spring with either carrot root sections or wooden plate traps enables growers to apply control treatments only if the population density justifies it. Egg parasitoids of the genus Anaphes (Hymenoptera: Mymaridae) are the most important natural enemies and they account, in untreated plots, for up to 80% mortality. Their effectiveness could be improved by managing field borders to establish secondary host species. An IPM programme was developed in the 1980s based on the research results obtained and, through regular monitoring, has achieved a substantial decrease in pesticide use.
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  • 16
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    Integrated pest management reviews 4 (1999), S. 39-52 
    ISSN: 1572-9745
    Keywords: Scapteriscus ; Neocurtilla ; Gryllotalpa ; biological control ; turf ; pastures ; vegetables
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract There are at least 70 species of mole crickets (Orthoptera: Gryllotalpidae). Some are rare, others are innocuous, and a few are important pests. These soil-dwelling pests damage underground parts of a long list of cultivated plants. Although tillage and flooding are used successfully in some situations to bring these pests to the soil surface and expose them to vertebrate and other predators, chemical pesticides are widely used against them. Knowledge of their life history is used to time application of chemical treatments to save money, but is not used as widely as it might be. Classical biological control has been used against immigrant mole crickets in Hawaii, Puerto Rico, and the southern USA. In Florida, three Scapteriscus species from South America cause major damage to pastures and turf and are targets of a classical biological control program. Population levels of two of the pest species have been reduced substantially in Florida by establishment of a tachinid fly (Ormia depleta) and a steinernematid nematode (Steinernema scapterisci) from South America. The nematode also functions as a biopesticide. Managers of pastures and turf in Florida have thus far derived benefit from these classical biological control agents without understanding their function: use of chemicals is reduced when mole cricket populations are lower due to action of these organisms. Future enhancement of the action of O. depleta and of a sphecid wasp (Larra bicolor, which also was introduced from South America) probably will demand deliberate planting of nectar sources for adults of these biological control agents, and the advantage will be to managers who adopt such a strategy. Chemical pesticide use is strongly promoted by a large chemical industry, whereas biopesticidal use has thus far been little promoted and sales have been few. Even managers who do not change their simple strategy of pesticide use in response to damage by mole crickets, and have no knowledge of the differing life cycles of the three Scapteriscus species or of the presence and action of the classical biological control agents, will derive benefit as these biological control agents (and a predatory beetle which has not yet been released) increase their distribution.
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  • 17
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    Integrated pest management reviews 4 (1999), S. 97-126 
    ISSN: 1572-9745
    Keywords: agriculture ; biological control ; campaign ; chemosterilent ; commensal ; control methods ; economics ; environmental and cultural methods ; horticulture ; India ; pest management ; pre- and post-harvest crop losses ; poultry farms ; rodent ; rodenticide ; South Asia ; trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Eighteen species of rodents are pests in agriculture, horticulture, forestry, animal and human dwellings and rural and urban storage facilities in India. Their habitat, distribution, abundance and economic significance varies in different crops, seasons and geographical regions of the country. Of these, Bandicota bengalensis is the most predominant and widespread pest of agriculture in wet and irrigated soils and has also established in houses and godowns in metropolitan cities like Bombay, Delhi and Calcutta. In dryland agriculture Tatera indica and Meriones hurrianae are the predominant rodent pests. Some species like Rattus meltada, Mus musculus and M. booduga occur in both wet and dry lands. Species like R. nitidus in north-eastern hill region and Gerbillus gleadowi in the Indian desert are important locally. The common commensal pests are Rattus rattus and M. musculus throughout the country including the islands. R. rattus along with squirrels Funambulus palmarum and F. tristriatus are serious pests of plantation crops such as coconut and oil palm in the southern peninsula. F. pennanti is abundant in orchards and gardens in the north and central plains and sub-mountain regions. Analysis of the information available on the damage and economic losses caused by rodents in rice, wheat, sugarcane, maize, pearl millet, sorghum, oil seed, legume and vegetable crop fields, horticulture and forestry, poultry farms, and rural and urban dwellings and storage facilities clearly shows that chronic damage ranging from 2% to 15% persists throughout the country and severe damage, sometimes even up to 100% loss of the field crop, is not rare. Several traditional and modern approaches and methods of rodent control are being used. The existing knowledge of the environmental, cultural, biological, mechanical and chemical methods of rodent control in India is reviewed. Considerable variations exist in the susceptibility of the pest species to different methods, particularly to rodenticides and trapping, their field applicability, efficacy and economics in different crops, seasons and geographical regions, behavioural responses of the pest species to these methods in different ecological conditions and their adoption by farmers in different regions of India. Environmental and cultural techniques, such as clean cultivation, proper soil tillage and crop scheduling, barriers, repellents and proofing which may reduce rodent harbourage, food sources and immigration have long lasting effects but are seldom adopted. However, their significance in relation to normal agricultural practices, intensification and diversification are discussed. Rodenticides, which provide an immediate solution to the rodent problem, form the major component of rodent control strategies in India. Poison baiting of rodents with zinc phosphide and burrow fumigation with aluminium phosphide are common in agricultural fields and recently Racumin (coumatetralyl) and bromadiolone have been introduced for the control of both agricultural and commensal rodent pests in India. Methods and timings of campaigns and successes and problems in implementation of rodent control are also reviewed.
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  • 18
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    Integrated pest management reviews 4 (1999), S. 307-312 
    ISSN: 1572-9745
    Keywords: microbial control ; biological control ; biopesticide ; mycopesticide ; Metarhizium ; Beauveria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Microbial control agents generally kill insects more slowly than chemical pesticides, and fast-moving migratory pests may not at first sight appear to offer the most promising targets for microbial control. Operators responsible for control may need to have recourse to chemical control agents. Nevertheless, there are many occasions when pests breed and feed outside the crop and a microbial control agent can be used. Similarly, immature stages may cause little damage and early treatment in the crop can avoid damage. Microbial control agents are particularly likely to be favoured if the pest breeds in a conservation area, and if a publicly-accountable agency is responsible for control. Other key points of importance are the IPM context, in particular detection, planning and forecasting of outbreaks and the role of natural enemies. With these points in mind, we identify several locust and grasshopper systems where microbial control is becoming established; additionally, Sunn pest of wheat and Armyworm are identified as promising situations forbreak microbials.
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    Journal of the history of biology 32 (1999), S. 509-555 
    ISSN: 1573-0387
    Keywords: Louis Agassiz ; Spencer Baird ; Charles Girard ; reputation ; taxonomy ; ichthyology ; Museum of Comparative Zoology ; Smithsonian Institution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , History
    Notes: Abstract The reputations of scientists among their contemporaries depend not only on accomplishment, but also on interactions affected by influence and personality. The historical lore of most fields of scientific endeavor preserve these reputations, often through the identification of founders, innovators, and prolific workers whose contributions are considered fundamental to progress in the field. Historians frequently rely on the historical lore of scientists to guide their studies of the development of ideas, exhibiting justifiable caution in reassessing reputations in the light of current knowledge. However, the transmission of historical lore can obscure the relative importance of accomplishment, influence and personality in shaping contemporary reputations, leaving the historian to either accept reputations at face value or attempt to reconstruct the context in which they were created. The science of taxonomy, because of its rules of priority, leaves a relatively accurate record of historical accomplishment through the persistence of taxa in catalogues and faunal guides. These records allow the modern historian an unbiased means to assess the relative accomplishments of historical figures and therefore a means to critically reassess reputations independent of personality and influence. In the historical lore of North American ichthyology, Louis Agassiz at Harvard and Spencer Baird at the Smithsonian emerge as central figures in the early development of the field during the mid-1800s, contributing not only through the quality and quantity of their science, but also through their roles as institutional leaders and mentors to workers who followed. Charles Girard, originally a student of Agassiz's and later a coworker with Baird, receives little notice in the history of ichthyology, and his reputation is that of a minor player in the initial description of the North American fish fauna, and one whose work appears to have been flawed or even careless when compared to his contemporaries. However, a review of both contemporary and modern taxonomic works reveals that Girard's productivity far exceeded that of either Agassiz or Baird. Furthermore, an examination of the tendency of Girard and his contemporaries to introduce synonymous names into the literature, which might reflect careless or uncritical work, suggests that Girard was among the more accomplished workers of hisera, including Agassiz and Baird. Girard's low ranking in the folklore of North American ichthyology, therefore, can not be attributed to discernible shortcomings in his scientific work, but rather to a public and private campaign of criticism waged by Agassiz after Girard's departure from Harvard. While Agassiz's dispute with Girard stemmed from their personal interactions, he expressed them as criticisms of Girard's work, and thus helped shape Girard's scientific reputation as it has been transmitted through the lore of ichthyology. This case study reveals how scientific reputation may not always rest on accomplishment, but can be influenced by personal interactions obscured by time but nonetheless important to history.
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    Journal of chemical ecology 25 (1999), S. 1555-1565 
    ISSN: 1573-1561
    Keywords: Yeasts ; Botrytis cinerea ; apple ; volatiles ; gray mold ; biological control ; fungal interaction ; acetate esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The fungus, Botrytis cinerea, causes decay in apples in postharvest storage. Conidia of B. cinerea on polycarbonate membranes were incubated on filter paper disks saturated with water or suspensions of yeasts (2 × 106 CFU/filter) and then exposed for 24 hr at 22°C to 0–16 μl of ethyl, butyl, or hexyl acetates injected into the headspaces of 500-cc glass jars. Germination of conidia was increased by exposure to 4–16 μl of ethyl acetate compared to the no-ester controls. Conidia were stimulated to germinate by 4 μl of butyl acetate and 8 μl of hexyl acetate, but greater volumes were toxic to germination. The yeasts Cryptococcus laurentii and Sporobolomyces roseus were more effective at reducing the stimulatory effect than Saccharomyces cerevisiae. Acetate esters also stimulated germination of conidia on polycarbonate membranes on apples inside jars. Cryptococcus laurentii or S. roseus suppressed this effect more than S. cerevisiae. Germination of conidia on a membrane on water inside a 3.9-liter jar containing an apple was 2.5-fold greater than the no-apple control. Butyl acetate increased Botrytis infection of apple wounds. Butyl and hexyl acetate were detected by GLC in the headspace of Golden Delicious apples. Results indicated that some acetate esters produced by apple stimulated germination of B. cinerea conidia, and this effect was suppressed by yeasts.
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  • 21
    ISSN: 1871-4528
    Keywords: bioassay ; biological control ; blackleg ; green crop harvesting ; haulm killing ; skin damage ; wound protection ; Solanum tuberosum L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Green crop lifting (GCL) for haulm killing was developed in The Netherlands and offers ideal conditions for controlling the blackleg pathogenErwinia carotovora subsp.atroseptica (Eca) by antagonists. Based on the use of mini-tubers or young tubers from field crops, two bioassays for wound protection were developed. GCL was simulated by artificially skinning or wounding tubers, inoculating the damaged skin with Eca, treating with antagonists and incubating in either potting compost or outside in field soil. Mainly fluorescent pseudomonads, pre-screened for in vitro antagonistic activity on agar and high soft rot reducing ability on tuber slices, were tested in the mini-tuber bioassay. Strains giving the highest degree of wound protection were further tested individually and in combination under field conditions in the young tuber bioassay. One individual strain and two combinations, resulting in reduction of contamination levels on skinned surfaces of 85% and between 60% and 70%, respectively, show good potential for biological control of blackleg.
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    Mycoscience 40 (1999), S. 73-80 
    ISSN: 1618-2545
    Keywords: Mycena auricoma ; Mycena sect.Radiatae ; Mycena spinosissima ; taxonomy
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    Topics: Biology
    Notes: Abstract Two lignicolous species ofMycena (Agaricales, Basidiomycetes) are described and illustrated from eastern, Japan:Mycena auricoma sp. nov., forming ephemeral coprinoid basidiomata and belonging to sectionRadiatae, was found on a dead fallen twig ofQuercus serrata. It appears to close to a Malaysian species,“Trogia” crinipelliformis. Mycena spinosissima in sectionSacchariferae, new to Japan, was collected from dead bark ofAphananthe aspera, a dead fallen inflorescence ofCryptomeria japonica, and a dead fallen twig ofQuercus serrata.
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  • 23
    ISSN: 1618-2545
    Keywords: cellular slime molds ; dictyostelids ; macrocyst ; mating system ; taxonomy
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    Topics: Biology
    Notes: Abstract Macrocyst formation in the sexual cycle was found in three dictyostelid species:Dictyostelium monochasioides, Polysphondylium candidum, andP. pseudo-candidum. Mating tests suggested thatD. monochasioides andP. pseudocandidum were heterothallic andP. candidum was homothallic. The primary walls of macrocysts had partially or fully degenerated, while the inner walls, believed to be tertiary walls, showed an undulate structure.
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    Mycoscience 40 (1999), S. 189-191 
    ISSN: 1618-2545
    Keywords: bamboo fungus ; hyperparasitism ; hyphomycetes ; mycoparasites ; taxonomy
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    Topics: Biology
    Notes: Abstract Cryptophiale sphaerospora sp. nov. is described and illustrated based on a single collection attached to a synnematous fungus,Janetia synnematosa, from a dead bamboo culm. It differs from other species ofCryptophiala in having spherical to subspherical conidia and a cerebroid layer of phialides. The overall morohology of this species is smaller than that of previously described species.
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  • 25
    ISSN: 1615-6110
    Keywords: Bryophyta ; Mitochondria ; nad5 gene ; group I intron ; molecular phylogeny ; taxonomy
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    Topics: Biology
    Notes: Abstract In contrast to animals, the slowly evolving mitochondrial nucleotide sequences of plants appear well suited to investigate phylogenetic relations between old taxonomic groups. Analysis ofnad5 gene sequences in 47 bryophytes, the living representatives of very early land plants, confirm this assessment. Statistically reliable phylogenetic trees are obtained with different mathematical approaches. A group I intron sequence conserved in thenad5 gene of all 30 mosses and 15 liverworts investigated supports a sister group relationship of the two classes. The intron sequence adds phylogenetic information for fine resolution on top of the conserved exon sequences down to the level of classically defined orders or families, respectively. This intron is not present in the hornwortsAnthoceros husnotii andA. punctatus. The results allow statements on diverging taxonomic interpretations and support the monophyly of the liverworts, mosses, Jungermanniidae, Marchantiidae and Bryidae, and allow recognition of subclasses like Hypnanae and Dicrananae. Among the mosses, the derived orders (subclass Bryidae) are confidently set apart from the Sphagnales, Andreaeales, Polytrichales and Tetraphidales with Buxbaumiales occupying a mediating position. Among the liverworts, full support is found for the classic separation of simple (jungermanniid) and complex thalloid (marchantiid) species with a strikingly low mitochondrial sequence divergence among the latter.
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    Journal of applied phycology 11 (1999), S. 411-419 
    ISSN: 1573-5176
    Keywords: Bangiales ; Porphyra life history ; culture ; conchocelis ; conchosporangia ; spores ; neutral conchospores morphology ; taxonomy
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    Topics: Biology
    Notes: Abstract Since 1992, as part of a study to circumscribe the genus Porphyra in New Zealand, an extensive culture programme has been developed at the Museum of New Zealand Te Papa Tongarewa. This collection currently houses more than 800 cultures and 150strains. We currently recognise at least 20 species in New Zealand, most of which are undescribed. Close observation of reproduction and the development of conchocelis-phase filaments grown in the laboratory, under controlled conditions of temperature, irradiance and photoperiod, has enabled the recognition of characters that can assist in species separation. The comparative taxonomic value of a range of such characters is discussed, including reporting a novel reproductive mode.
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  • 27
    ISSN: 1573-5176
    Keywords: distribution ; harvesting ; Porphyra ; recruitment ; seasonality ; South Africa ; taxonomy
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    Topics: Biology
    Notes: Abstract Although Porphyra is commercially farmed in many countries, in South Africa only small harvests of wild populations for sale as nori have been carried out. The discovery that Porphyra improves growth of South African abalone (Haliotis midae) farmed inland-based tanks has led to increased pressure to harvest wild populations. This paper reports on a survey of the distribution and seasonality of Porphyra in the southern Western Cape. Porphyrawas present at all sites surveyed, and showed considerable temporal variation. A significant amount of the Porphyra present is in reserves and therefore protected from harvesting. Close rexamination of one site revealed seasonal populations of Porphyra that occupied different niches dependent on season. Recruitment peaked in spring and autumn, leading to dense summer and winter populations. Summer populations generally grew lower in the eulittoral than winter populations. No pattern in the mortality of larger thalli wasde tected, though sporeling mortality was high following recruitment peaks. Although it seems that most sites in the southern Western Cape are suitable for harvesting, the taxonomy of the genus in the region urgently needs revision if populations are to be appropriately managed.
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    Mycoscience 40 (1999), S. 165-183 
    ISSN: 1618-2545
    Keywords: freshwater fungi ; Halosphaeriaceae ; taxonomy ; tropics
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    Topics: Biology
    Notes: Abstract The generic concepts inAniptodera, Halosarpheia, Nais andPhaeonectriella are discussed and those species occurring in freshwater are described, some with illustrations. Five new species:Aniptodera lignicola, A. mauritaniensis, A. megalospora, A. palmicola, Phaeonectriella appendiculata are also described. A key to the freshwater species in the above genera and a synoptic diagram of their ascospores are provided.
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  • 29
    ISSN: 1618-2545
    Keywords: Ascomycota ; marine fungi ; new species ; taxonomy
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    Notes: Abstract A new speciesDactylospora mangrovei is described and illustrated and compared withD. haliotrepha andD. canariensis, all marine inhabiting species.Dactylospora haliotrepha andD. mangrovei are tropical/sub-tropical mangrove species collected in Hong Kong, Malaysia, Taiwan and Thailand, whileD. canariensis was described from temperate waters. A map of the geographical distribution of the three marineDactylospora species is presented.
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    Mycoscience 40 (1999), S. 185-188 
    ISSN: 1618-2545
    Keywords: Amphisphaeria ; ascomycetes ; bamboo fungi ; taxonomy
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    Topics: Biology
    Notes: Abstract A new species ofArecophila and a species previously known asAmphisphaeria coronata are described and illustrated from dead culms of bamboo.Arecophila bambusae sp. nov. is distinct in the genus in having ellipsoidal ascospores with slightly round ends, and asci with a narrow subapical ring.Arecophila coronata comb. nov. has asci with a wedgeshaped apical ring and weakly striated ascospores enclosed in wide mucilaginous sheath.
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  • 31
    ISSN: 1573-1464
    Keywords: Adelges tsugae ; biological control ; Diapterobates humeralis ; hemlock ; Japan ; natural enemies ; Picea spp. ; population dynamics ; predators ; Pseudoscymnus tsugae ; Tsuga spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hemlock woolly adelgid, Adelges tsugae Annand (Homoptera: Adelgidae), is native to Japan where it is an innocuous inhabitant of Tsuga diversifolia Masters and T. sieboldii Carriere throughout their natural growing areas. Native adelgid populations are regulated by host resistance and natural enemies, in particular the oribatid mite, Diapterobates humeralis (Hermann) and the coccinellid beetle, Pseudoscymnus tsugae Sasaji and McClure. Invading populations of A. tsugae in western North America on T. heterophylla Sargent and T. mertensiana Carriere are mainly regulated by host resistance. However, invading populations in eastern North America attain damaging levels on T. canadensis (L.) Carriere and T. caroliniana Engelmann and are regulated mainly by weather and negative density-dependent feedback mechanisms related to host deterioration. Although A. tsugae is only passively dispersed by wind, birds, forest-dwelling mammals and humans, it is spreading at an alarming rate and is sufficiently cold hardy to threaten the existence of the two eastern hemlock species throughout their natural ranges. The current hope for suppressing invading populations of hemlock woolly adelgid in eastern North America lies with the exotic predator, P. tsugae. Extensive laboratory studies of the biology and predatory ability of P. tsugae revealed that it feeds on all life stages of its prey, that its multivoltine life cycle is well synchronized with that of the adelgid, and that it has great potential for biological control. We have reared and released nearly 130,000 adults of P. tsugae in forests in Connecticut, New Jersey and Virginia during the past four years. P. tsugae has reproduced, dispersed, overwintered and reduced densities of hemlock woolly adelgid by 47–88% in only five months on release branches at these sites. Current studies are investigating the long-term ability of P. tsugae to regulate invading populations of A. tsugae in eastern North America.
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    Biological invasions 1 (1999), S. 301-311 
    ISSN: 1573-1464
    Keywords: Alliaria petiolata ; biological control ; garlic mustard ; invasions ; Lythrum salicaria ; monitoring ; Phragmites australis ; purple loosestrife
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    Topics: Biology
    Notes: Abstract The invasion of non-indigenous plants is considered one of the primary threats to rare and endangered species as well as to the integrity and function of North American ecosystems. However, many of the suspected negative ecosystem impacts are based on anecdotal evidence. For example, there is almost unanimous agreement among natural resource managers of the detrimental ecological impacts of species such as Lythrum salicaria (purple loosestrife), Phragmites australis (common reed) and Alliaria petiolata (garlic mustard) but convincing documentation is scarce. Experimental and theoretical ecology predicts large ecosystem impacts of the most widespread invasive species. However, it is difficult to prioritize control of species that occur at intermediate densities. Long-term monitoring before and during the invasion as well as before, during and after any control attempts can provide valuable ecological information. In particular, it is important to understand how changes in the abundance of species influence ecosystem properties and processes which, in turn, will help guide management decisions. Ideally, this monitoring has to go beyond 'simple’ impacts on plant communities, involve cross-disciplinary teams of scientists and should incorporate many different taxa and their interactions. Monitoring design and data collection should be sophisticated enough to allow statistically sound data analysis. The available information will be paramount in (1) developing new political and scientific guidelines in invasive species management, (2) helping resolve potential conflicts of interest and (3) helping change public attitudes regarding growth, sale, and control of non-indigenous species.
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  • 33
    ISSN: 1573-1464
    Keywords: biological control ; biological invasions ; colonization ; Galerucella calmariensis ; Galerucella pusilla ; population ; establishment ; release size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Introductions of biological control organisms offer a unique opportunity to experimentally study the process of invasion by exotic species. I used two chrysomelid beetles, Galerucella calmariensis and Galerucella pusilla, which are currently being introduced into North America for the biological control of purple loosestrife (Lythrum salicaria), to determine how the initial size of a release affects the probability that the introduced population grows and persists. I released both species into stands of their host plant at 36 sites scattered throughout central New York State using four release sizes: 20, 60, 180, and 540. I returned to these sites over the next 3 years to census the populations. For both species, the probability of population establishment increased with release size. Population growth rates also depended positively on release size. The implication from these results is that the demographic factors whose influence depends on population size or density such as demographic stochasticity, Allee effects, and genetics play important roles in the establishment of invading populations. A second set of releases was used to determine if it was at all possible for a single gravid female to found a population. Out of twenty individual females released, one female (a G. calmariensis) founded a population that persisted until the end of the study (3 generations).
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    European journal of plant pathology 105 (1999), S. 123-137 
    ISSN: 1573-8469
    Keywords: Aureobasidium pullulans ; biological control ; Cryptococcus albidus ; grey mould ; Trichoderma harzianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The efficacy of Trichoderma harzianum T39 and the yeasts Aureobasidium pullulans and Cryptococcus albidus against Botrytis cinerea in cucumber and tomato was compared with chemical control. Four experiments were conducted in cucumber grown under different climatic conditions in The Netherlands, and two experiments were done in tomato both in the Netherlands and in Israel. T. harzianum and A. pullulans showed the most consistent control of B. cinerea, reducing stem lesions and death of plants by 40–100% in most cases. Control of stem lesions and subsequent wilting was generally better than control of symptoms on fruits. In some cases, the biocontrol agents were more effective than the broad-spectrum fungicide tolylfluanid and the selective fungicide iprodione. The climatic conditions did not strongly influence the efficacy of the biocontrol agents, but regression analysis showed that high temperature during the day and high vapour pressure deficit during the night reduced biocontrol efficacy. From the results, prospects for biocontrol of B. cinerea in greenhouse vegetables appear good under a range of conditions.
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    European journal of plant pathology 105 (1999), S. 95-101 
    ISSN: 1573-8469
    Keywords: bacteria ; biological control ; Chryseobacterium ; Flavobacterium ; grey mold ; Pseudomonas ; strawberries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Most potential fungal antagonists have been identified only after exhaustive non-selective screening. Bacteria antagonistic to Botrytis cinerea were selected using a simple method based on techniques developed for trace enrichment studies, where a crude cell wall preparation from B. cinerea was used as a selective substrate and bacteria were isolated based on growth on cell wall agar media. Most of the 52 bacterial isolates that were obtained from the surface of ‘organically grown’ strawberry fruit and could grow on B. cinerea wall media in culture showed some ability to inhibit the growth of the fungus. Potato dextrose agar seeded with B. cinerea spores (104 ml−1) were challenged with each of these bacterial isolates at concentrations of 109 and 106 colony forming units ml−1. Eleven of the 52 isolates initially recovered, subsequently demonstrated strong antagonism in vitro and were selected for additional screening tests on strawberry fruit. All 11 isolates reduced grey mold rot incidence on fruit in storage. Three of the best isolates were tested in limited field trials, and also reduced grey mold rot on fruit under field conditions.
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  • 36
    ISSN: 1573-8469
    Keywords: in vitro interactions ; biological control ; rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fusarium wilt diseases, caused by the fungus Fusarium oxysporum, lead to significant yield losses of crops. One strategy to control fusarium wilt is the use of antagonistic, root-colonizing Pseudomonas spp. It has been demonstrated that different strains of these bacteria suppress disease by different mechanisms. Therefore, application of a mixture of these biocontrol strains, and thus of several suppressive mechanisms, may represent a viable control strategy. A prerequisite for biocontrol by combinations of biocontrol agents can be the compatibility of the co-inoculated micro-organisms. Hence, compatibility between several Pseudomonas spp. strains, that have the ability to suppress fusarium wilt of radish, was tested in vitro on KB agar plates. Growth of P. fluorescens strain RS111 was strongly inhibited by Pseudomonas spp. strains RE8, RS13, RS56 and RS158, whereas a mutant of strain RS111 (RS111-a) was insensitive to inhibition by these strains. Strains RS111 and RS111-a only slightly inhibited some other strains. Suppression of fusarium wilt of radish in a potting soil bioassay by the incompatible combination of RE8 and RS111 was comparable to the effects of the single strains. However, disease suppression by the compatible combination of RE8 and RS111-a was significantly better as compared to the single strains. In contrast, the incompatible combination of RS56 with RS111 resulted in enhanced disease suppression as compared to the single strains. Increased disease suppression by combinations of RS13 or RS158 with RS111 or RS111-a was not observed. This indicates that specific interactions between biocontrol strains influence disease suppression by combinations of these strains.
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    Journal of systems integration 9 (1999), S. 167-185 
    ISSN: 1573-8787
    Keywords: flexibility ; complexity ; systems approach ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract In this paper we present a taxonomy of manufacturing problems, labeled in a general sense as Design, Production, or Distribution problems. One or more basic systems concepts, such as complexity and adaptation, attach themselves to each such problems. By combining the hierarchical Design—Production—Distribution idea with system concepts, we establish the fact that there is, indeed, a significant systems component to most problems of modern manufacturing.
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    Plant systematics and evolution 214 (1999), S. 91-102 
    ISSN: 1615-6110
    Keywords: Asteraceae ; Lactuceae ; Aetheorhiza ; Launaea ; Reichardia ; Sonchus ; Pollen ; taxonomy ; harmomegathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pollen morphology of the taxa belonging to the generaAetheorhiza Cass.,Launaea Cass.,Reichardia Roth andSonchus L. in the Iberian Peninsula has been studied with light and electron microscopy. The pollen is 3(-4)-zonocolporate and echinolophate (without polar lacunae, but in general with prelacunae), with equatorial ridges and 15–20 lacunae: 3–4 poral, 6–8 abporal and 6–8 paraporal. Small to medium size, P × E = 19−36 × 23–42 µm; sometimes two different sizes have been found. Exine 3–9 µm thick and ornamentation microreticulate and echinate. The results clearly show the relationships between genera.
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    Plant systematics and evolution 214 (1999), S. 161-186 
    ISSN: 1615-6110
    Keywords: Rubiaceae ; Rubioideae ; Angiosperms ; cladistics ; DNA sequences ; phylogeny ; rps16 intron ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phylogeny of the subfamilyRubioideae (Rubiaceae) was estimated from sequence variation in therps16 intron (cpDNA) in 143 ingroup and 5 outgroup taxa. The analysis largely confirms a recent one based onrbcL sequences, but branch support is often much stronger. Three of the traditional subfamilies are supported,Rubioideae, Cinchonoideae s. str., andIxoroideae s. l. while there is no support forAntirheoideae. TheRubioideae are the sister group of all otherRubiaceae and comprise the tribesAnthospermeae, Coccocypseleae, Cruckshanksieae, Coussareeae, Gaertnereae, Hedyotideae, Knoxieae, Morindeae, Ophiorrhizeae, Paederieae, Pauridiantheae, Perameae, Psychotrieae, Rubieae, Spermacoceae, Theligoneae, andUrophylleae. TheHamelieae andHillieae belong to theCinchonoideae. Rachicallis andSiemensia should be transferred from theHedyotideae to theCinchonoideae. ThePauridiantheae, Urophylleae, Ophiorrhizeae, andRaritebe form the basalmost subclade of theRubioideae. The second basalmost clade consists of the generaLasianthus andPerama. The third basalmost clade consists of the tribesCoussareeae, Coccocypseleae andCruckshanksieae, and the generaDeclieuxia andHindsia. The tribesKnoxieae, Anthospermeae, Argostemmateae, Paederieae, Theligoneae, Rubieae, Hedyotideae, andSpermacoceae are members of one clade. TheKnoxieae are monophyletic ifOtiophora, Otomeria, andPentas are included. The tribeAnthospermeae is supported as monophyletic, but its subtribes are not. ThePaederieae, together withTheligonum, form a paraphyletic grade basal to theRubieae. TheHedyotideae, includingSchismatoclada, form a grade at the base of theSpermacoceae. TheGaertnereae are monophyletic and distinct from thePsychotrieae. TheMorindeae are monophyletic and includeDamnacanthus andMitchella. Schradera is the sister group of theMorindeae. ThePsychotrieae are monophyletic when theGaertnereae, Lasianthus, andDeclieuxia are excluded. The recognition of a subtribeHydnophytineae leaves the rest of thePsychotrieae paraphyletic.Psychotria is paraphyletic with respect to all other genera of the tribe. Approximately 50 genera are here classified for the first time based on molecular data.
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    Plant systematics and evolution 219 (1999), S. 199-208 
    ISSN: 1615-6110
    Keywords: Brexioideae ; Brexia ; Ixerba ; Roussea ; Saxifragaceae s. l. ; 18S rDNA andrbcL sequence analysis ; molecular systematics ; phylogenetic analysis ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous molecular phylogenetic analyses have demonstrated that Saxifragaceae sensu lato are polyphyletic, with component lineages scattered throughout the eudicots. As part of our effort to elucidate the relationships of members of Engler and Prantl's Saxifragaceae s. l., we undertook a molecular systematic study of subfamily Brexioideae, which comprises three genera:Brexia, Ixerba, andRoussea. Not all taxonomic treatments have concurred, however, in placing these genera together. To elucidate relationships among these three genera as well as their relationships to other angiosperms we constructed large data sets ofrbcL, 18S rDNA, andrbcL + 18S rDNA sequences. Our phylogenetic analyses indicate clearly that Brexioideae are polyphyletic.Brexia is part of a celastroid clade that also includesParnassia, Lepuropetalon, and Celastraceae.Ixerba appears as sister to a large eurosid I clade;Roussea appears as part of Asterales. Molecular data, therefore, indicate that Brexioideae are a polyphyletic assemblage and component genera should ultimately be incorporated into other groups. Our studies continue to demonstrate the polyphyly not only Saxifragaceae s. l., but also of its constituent subfamilies.
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  • 41
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    Genetic resources and crop evolution 46 (1999), S. 143-147 
    ISSN: 1573-5109
    Keywords: Amygdalus ; distribution ; domestication ; taxonomy ; wild relatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Almond, Amygdalus communis L., is an ancient crop of south west Asia. Selection of the sweet type marks the beginning of almond domestication. Wild almonds are bitter and eating even a relatively small number of nuts can be fatal. How man selected the sweet type remains a riddle. Also, the wild ancestor of almond has not been properly identified among the many wild almond species. Breeding experiment, which is the most critical test for identifying the wild progenitors of other crops, is ineffective in almond, because it is interfertile with many wild taxa. The so-called wild A. communis of central Asia cannot be regarded as a genuine wild form, but as a feral form, or remains of old afforestation. The wild taxa morphologically akin to almond, A. korshinskyi (H.-M.) Bomm. and A. webbii Spach, are also feral types occurring in the Middle East and southern Europe, respectively. The taxon A. fenzliana (Fritsch) Lipsky is the most likely wild ancestor of almond for three reasons: 1. It is a genuine wild type forming extensive thickets of large trees young seedlings and all the intergradations between them in nature; 2. Its morphology, and particularly the partially pitted grooved nut-shell are within the range of variation of almond, and 3. A. fenzliana is native of Armenia and western Azerbaijan in the Middle East where almond was apparently domesticated.
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  • 42
    ISSN: 1573-5109
    Keywords: evolution ; genetic resources ; PCR-RFLP ; RAPDs ; Vicia ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We report the results of two methods of DNA analysis to elucidate phylogenetic relationships among 29 Vicia subgen. Vicia species in comparison with two species of subgenus Vicilla sect. Vicilla. The methods employed were RAPD analysis of total genomic DNA and PCR-RFLP analysis of five chloroplast genes, rbcL, rpoB, 16S, psaA and trnK. The results of each method were similar and complementary, and support the current taxonomic systems of subsp. Vicia. According to RAPD and PCR-RFLP analysis the Narbonensis complex can be considered a well separated section, which may be related to section Vicia. Sections Vicia, Atossa and Wiggersia are separate, but closely related sections. Species of the section Hypechusa form a single monophyletic section, where V. lutea, V. anatolica and V. hyrcanica are quite remote from other species. Our results suggest that within the subgenus Vicia, V. faba is more closely related to V. bithynica and that these two species are most closely related to section Peregrinae. We found that PCR-RFLP of cp DNA provided more precise information concerning relationships between Vicia sections than RAPD analysis. However, RAPD analysis was more informative concerning diversity of closely related Vicia taxa, such as the variable groups, section Narbonensis and V. sativa aggregate.
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  • 43
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    Genetic resources and crop evolution 46 (1999), S. 207-211 
    ISSN: 1573-5109
    Keywords: introduced species ; karyotypes ; taxonomy ; Vicia sativa agg
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract V. sativa L. sensu strictu was introduced in Rio Grande do Sul (Southern Brazil) by Italian settlers and is nowadays cultivated for forage in backyards and fields. V. angustifolia L. is a widespread highly polymorphic naturalized ruderal. Both taxa grow sympatrically and morphologically transitional types are often found. We analyzed 37 accessions of V. sativa, V. angustifolia, intermediate types, and V. cordata Wulf. ex. Hoppe from Rio Grande do Sul, for karyotypes, corolla and legume color, seed color pattern, pod constrictions and phenological development. Karyotypes allowed a clear distinction between the taxa: V. sativa (2n=12) had a metacentric marker chromosome, V. angustifolia and intermediate types (2n=12) only acrocentric chromosomes and V. cordata presented 2n=10. Flowering and fruiting periods were shorter for V. sativa than for V. angustifolia, which was separated into early and late flowering groups. Qualitative characters separated the taxa by Jaccard coefficients into three groups: V. sativa at 0.5 similarity level, V. angustifolia and intermediate types at 0.6 and V. cordata joined V. sativa at 0.12. Our results show that karyotypes and a range of qualitative characters are diagnostic for the aggregate represented in Southern Brazil.
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  • 44
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    Hydrobiologia 403 (1999), S. 27-38 
    ISSN: 1573-5117
    Keywords: Cladocera ; Daphnia ; taxonomy ; homonymy ; phylogenetic relationships ; Patagonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Daphnia sarsi Daday, 1902 is redescribed from Patagonian populations and type material. Its taxonomic and nomenclatorial status is discussed. Because of the homonymy with D. sarsi Schödler, 1877, assigned to Australian populations, D. dadayana is proposed as new name. It is concluded that D. dadayana is a valid species with several morphological peculiarities and the proposed synonymies with D. carinata and D. similis can not be supported. Its subgeneric position is intermediate between Daphnia s. str. and Ctenodaphnia and there is an ontogenetical shift of the dorsocephalic suture from Ctenodaphnia-like to Daphnia-like. The possible origin of the group of species assigned to the subgenus Daphnia from a Ctenodaphnia-like ancestor is suggested.
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  • 45
    ISSN: 1573-5117
    Keywords: taxonomy ; marine nematodes ; Sabatieria ; the Strait of Magellan ; Chile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eight species of the genus Sabatieria, one of which is new to science, are described from the Strait of Magellan and the Beagle Channel, Chile. Sabatieria coomansi n. sp. is characterised by a rather long body (3236–4012 μm) with conspicuous lateral differentiation; head diameter 24 μm, amphids 2.25 turns; long spicules (132–149 μm) or 1.6–1.9 abd, 23–26 precloacal supplements, tail 3.3–3.6 abd. This species belongs to the praedatrix group (Platt, 1985). Sabatieria arcuata is found since its original description by Wieser in 1954. Six other species, i.e., S. celtica Southern, 1914, S. falcifera Wieser, 1954, S. furcillata Wieser, 1954, S. granifer Wieser, 1954, S. mortenseni (Ditlevsen, 1921) and S. parabyssalis Wieser, 1954 are redescribed in detail with emphasis on new or shortly described morphological features.
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  • 46
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    Hydrobiologia 394 (1999), S. 145-152 
    ISSN: 1573-5117
    Keywords: Heliodiaptomus ; H. elegans ; redescription ; synonymy ; H. serratus ; taxonomy ; Copepoda ; Calanoida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heliodiaptomus elegans Kiefer, 1935, incompletely described from Myanmar, is redescribed on specimens from Thailand. A Chinese congener, H. serratus Shen & Tai, 1962, is sunk into the synonymy of H. elegans.
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  • 47
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    Hydrobiologia 397 (1999), S. 21-28 
    ISSN: 1573-5117
    Keywords: Turbellaria ; taxonomy ; morphology ; Extremadura ; Spain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three new species of Rhabdocoela belonging to the Families Dalyellidae: Microdalyellia bipennata, and Typhloplanidae: Mesocastrada iberiensis, and Mesostoma extremeniensis are described. Fourteen species of the Orders Catenulida, Macrostomida, Proseriata and Rhabdocoela are cited the first time for Extremadura (southwest Spain).
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  • 48
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    Hydrobiologia 412 (1999), S. 25-34 
    ISSN: 1573-5117
    Keywords: Chirocephalus ; Anostraca ; taxonomy ; ecology ; Slovenia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Numerous specimens of Chirocephaluswere found repeatedly over the last eight years in an intermittent lake in SW Slovenia. They were first identified as Chirocephalus spinicaudatusSimon, 1886. Later it turned out that they fit the diagnosis of Chirocephalus croaticus (Steuer, 1899), a species described from a small lake in Southern Croatia, about 300 km from Slovenia. It was considered as endemic of its type locality only. Now its range expands into NW direction. The population in Slovenia is in good condition and, if there is no change in land use, could persist for a long time. A small population of the same species in a small puddle near the intermittent lake Cerkniško Jezero (about 30 km from lake Petelinjsko Jezero) was destroyed a few years ago. A detailed description of specimens of both sexes is given and some information on their ecology is added. Particular attention is given to the male legs which could provide good diagnostic characters. For comparison, legs of Chirocephalus diaphanus Prévost, 1803 are shown.
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  • 49
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    Hydrobiologia 412 (1999), S. 143-153 
    ISSN: 1573-5117
    Keywords: Cletocamptus merbokensis ; Harpacticoida ; taxonomy ; Malaysia ; mangroves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cletocamptus merbokensis sp. nov. is described from material collected out of muddy sediments in the Merbok mangrove forest, northwest peninsula Malaysia. It is distinguished from all other known species of Cletocamptus by the presence of only two setae on P2 and P3 endopod-2 and no inner seta on P4 exopod-2; and by the form of the male P5 in which the baseoendopod and most of the endopodal and exopodal lobes are absorbed into the somatic cuticle. The higher taxonomic position of the genus is briefly discussed.
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  • 50
    ISSN: 1573-5133
    Keywords: ecophenotypism ; branchial sieve ; geographic variation ; fish ; meristics ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A marked salinity-related decrease in gillraker number in sea bass samples during growth was documented. All specimens share the same genetic stock and rearing conditions were constant during early ontogeny. Variation in gillraker number could be related to the ecophenotypism of this character but selection can not be excluded. Results obtained from reared specimens were compared with those reported in the literature and with data collected from wild stocks. Attention is focused on the pitfalls that the use of this character may have on the taxonomy of fish species.
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  • 51
    ISSN: 1439-0361
    Keywords: Geographic variation ; taxonomy ; Rufous-tailed Hummingbird ; Amazilia tzacatl brehmi ; subspec. nov. ; Trochilidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Von der in Mittelamerika und in NW-Südamerika weitverbreiteten Braunschwanzamazilie (Amazilia tzacatl) wurden mehrere Unterarten beschrieben, von denen in der neueren Literatur drei anerkannt wurden:A. t. tzacatl (De la Llave, 1832) mit einem Vorkommen von NO-Mexiko bis W-Venezuela einschließlich N-Kolumbiens;A. t. jucunda (Heine, 1863) von W-Kolumbien bis SW-Ecuador; undA. t. handleyi (Wetmore, 1963) als Endemit der Panamá vorgelagerten Isla Escudo de Veraguas (Karibik). Letzteres Taxon wird von einigen Autoren als Allospezies angesehen, hier aber lediglich als Unterart bewertet, da offenbar nur morphometrische Unterschiede zu den übrigen Taxa bestehen. Die Ergebnisse der vorliegenden biogeographisch-taxonomischen Analyse belegen, daß die geographische Variation der Art im südlichen Verbreitungsgebiet größer ist als bislang angenommen wurde. Aufgrund signifikanter Unterschiede in den Gefiedermaßen erhält die kolumbianischvenezuelanische Population ihren früheren, eigenständigen taxonomischen Rang (A. t. fuscicaudata Fraser, 1840) zurück. Ein neues Taxon,Amazilia tzacatl brehmi subsp. nov., wird von Nariño, Südwestkolumbien, beschrieben. Diese Unterart tritt offenbar allopatrisch zuA. t. jucunda auf und unterscheidet sich morphometrisch deutlich von konspezifischen Nachbarpopulationen.
    Notes: Summary The Rufous-tailed Hummingbird (Amazilia tzacatl De la Llave, 1832), a widespread inhabitant of Central America and NW South America, currently comprises two subspecies (A. t. tzacatl andA. t. jucunda). Another taxon of the species complex isA. t. handleyi (Wetmore, 1963), initially described as an allospecies but here regarded only as a subspecies ofA. tzacatl. Based on diverging morphological features, the amount of geographical variation ofA. tzacatl in its South American range suggests the distinction of further populations. As a taxonomical consequence, we recognize the validity ofA. t. fuscicaudata (Fraser, 1840), and describe a new subspecies,A. t. brehmi subspec. nov., from Nariño, Colombia.
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  • 52
    ISSN: 1573-1561
    Keywords: Allelopathy ; Allium tuberosum ; antibacterial activity ; biological control ; intercropping ; Lycopersicon esculentum Mill ; Pseudomonas solanacearum E. F. Smith ; root exudates ; soil-borne diseases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Tomato plants were grown alone or intercropped with Chinese chive plants, with or without the inoculation of Pseudomonas solanacearum. Chinese chive plants had no detrimental effects on the growth of tomato plants but significantly delayed and suppressed the occurrence of bacterial wilt of tomato. P. solanacearum population decreased faster in the soil grown with tomato alone than that in the soil grown with both tomato and Chinese chive. However, P. solanacearum population in bare soil was higher than that grown with Chinese chive. Root exudates of Chinese chive collected with a continuously trapping system were inhibitory to multiplication of P. solanacearum.
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  • 53
    ISSN: 1573-1561
    Keywords: Herbivore-induced synomones ; infochemicals ; semiochemicals ; learning ; biological control ; prey location ; behavior ; Acarina ; Phytoseiulus persimilis ; Tetranychus urticae ; Gerbera jamesonii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract When leaves of the ornamental crop Gerbera jamesonii are damaged by the spider mite Tetranychus urticae, they produce many volatile compounds in large quantities. Undamaged gerbera leaves produce only a few volatiles in very small quantities. In the headspace of spider mite-damaged gerbera leaves many terpenoids are present, comprising 65% of the volatile blend. In addition, a number of nitrogen containing compounds, such as oximes and nitriles, are produced. We studied the attraction of P. persimilis to the volatiles from spider mite-damaged gerbera leaves and how attraction is affected by starvation and previous experience. Phytoseiulus persimilis that were reared on spider mites (T. urticae) on Lima bean were not attracted to spider mite-induced volatiles from gerbera. Starvation did not influence the predator's response to these volatiles. In contrast, predators that were reared on spider mites on gerbera leaves were strongly attracted to volatiles from spider mite-infested gerbera. This was found also for predators that originated from a culture on spider mite-infested bean and were offered six days of experience with spider mites on gerbera leaves.
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  • 54
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    World journal of microbiology and biotechnology 15 (1999), S. 545-552 
    ISSN: 1573-0972
    Keywords: Fermentation ; polyene antifungal antibiotic ; Streptomyces ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A Streptomyces strain UK10 was isolated from Ukrainian soil and identified by taxonomical studies as Streptomyces arenae var ukrainiana. HA-2-91 was isolated from the biomass of S. arenae var ukrainiana and is supposedly a polyene macrolide antibiotic belonging to the tetraene group. HA-2-91 showed promising antifungal activity (in vitro) against yeasts and filamentous fungi, including plant pathogens and dermatophytes and was found to be less toxic in mice than nystatin and rimocidin.
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  • 55
    ISSN: 1573-0832
    Keywords: antagonism ; biological control ; modified dual plate culture method (MDPCM) ; Pythium acanthicum ; P. oligandrum ; P. periplocum ; P. ultimum var. ultimum ; sclerotia bait technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mycoparasitic Pythium species with spiny oogonia were surveyed in 50 Palestinian agricultural fields subject to different cropping practices using the Sclerotia Bait Technique (SBT) and the Surface-Soil-Dilution-Plate method (SSDP) with the selective VP3 medium. The mycoparasitic Pythium species were obtained from 21 (42%) soils using the SSDP method and from 37 (74%) soils using SBT. Pythium acanthicum and P. oligandrum were isolated by both methods, whereas P. periplocum was isolated only by the SBT. Using a newly modified dual plate culture method (MDPCM), the three mycoparasites showed varying antagonistic performance against several Pythium host species under a range of in vitro conditions. However, P. periplocum and P. oligandrum were found to be active biocontrol agents against P. ultimum, the damping-off organism of cucumber. This pathogen was antagonized, on thin films of water agar, by the three mycoparasites, and was moderately susceptible to P. periplocum while slightly susceptible to P. acanthicum and P. oligandrum. In direct application method in which antagonistic mycoparasites were incorporated into peat/sand mixture artificially infested with P. ultimum under growthroom conditions, Pythium oligandrum and P. periplocum (at 500 CFUg−1) significantly improved seedling emergence and protected seedlings from damping-off. In the seed coating method, biocontrol by two types of seed dressing (homogenate- or oospore coated seeds), was comparable to that achieved by direct application.
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    Experimental and applied acarology 23 (1999), S. 281-296 
    ISSN: 1572-9702
    Keywords: bodyguards ; plant–predator mutualism ; tritophic interactions ; castor bean ; phytoseiids ; life history ; pollen ; banker plant ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phytoseiid mite Iphiseius degenerans (Berlese) is an effective predator of western flower thrips, Frankliniella occidentalis (Pergande), in Dutch greenhouses. In the Mediterranean area, castor bean, Ricinus communis L., is known as a year-round host plant for this predatory mite. On flowering castor bean plants in greenhouses, I. degenerans can be found in densities of more than 100 per leaf. For this reason, the plant is being used as a ‘banker’ plant to augment biological control. It has been shown that pollen produced by the large apical flowers sustains reproduction and development for these mites. The objective of this study was to measure the contribution of the extrafloral nectar of this plant to the reproductive success of this predatory mite. A study conducted at 25°C in presence of free water showed that (1) I. degenerans is unable to develop beyond the protonymphal stage when fed only nectar and leaf tissue, (2) its ovipositional rate is higher when pollen is supplemented with nectar, (3) its reproduction ceases within a few days when fed on nectar only, but the predator can survive for several weeks and resume oviposition when fed pollen again and (4) the feeding of young females for one or two weeks with nectar only extends their longevity by approximately the same period and only slightly diminishes their lifetime reproductive potential (R0), as compared to mites continuously fed pollen. It can be concluded that extrafloral nectar can provide an important contribution to population growth and maintenance of I. degenerans on R. communis, particularly in pre- and post-blooming periods. Assuming these predators are beneficial to the plant in clearing them of herbivorous mites and thrips, this relationship may be regarded as an example of plant–predator mutualism. The combination of pollen and extrafloral nectar makes castor bean an ideal rearing and banker plant for I. degenerans.
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    Experimental and applied acarology 23 (1999), S. 741-763 
    ISSN: 1572-9702
    Keywords: biological control ; interspecific competition ; leaf hairiness ; grapevine ; Tetranychidae ; Eriophyidae ; Phytoseiidae ; Stigmaeidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a three-year study, mite populations were monitored in two vineyards, each having two grape varieties with different leaf hair density. In both vineyards native phytoseiids were present: Amblyseius andersoni in one vineyard, and Phytoseius finitimus in the other. The economically important predators Kampimodromus aberrans and Typhlodromus pyri were released in both vineyards in order to study their efficacy in controlling tetranychids and eriophyids and their persistence during periods of prey scarcity. In both vineyards, relative abundances of the mite species, especially phytoseiids, were found to differ on different varieties in the same vineyard. In the first experiment, A. andersoni reached higher densities and was more persistent on the variety with slightly pubescent leaf under-surface (Merlot). Typhlodromus pyri and K. aberrans releases were successful and the mites became more abundant on the variety with pubescent leaf under-surface (Verduzzo). In the second experiment, P. finitimus was more abundant on a variety with pubescent leaf under-surface (Prosecco) than with glabrous leaf under-surface (Riesling). The most interesting results of the present study concerned the interactions between native and released predators. In the first vineyard, different results were obtained when releasing T. pyri on the two varieties. On the variety with pubescent leaves, A. andersoni was rapidly displaced by T. pyri, whereas the former species persisted on the other variety throughout the three-year study, apparently becoming dominant during the last season. In contrast to T. pyri, interactions between K. aberrans and A. andersoni in this vineyard did not depend on variety. The results of the experiments carried out in the second vineyard stressed the importance of interspecific competition for phytoseiid releases. Typhlodromus pyri colonization failed on both varieties. Kampimodromus aberrans releases appeared to be more successful on Riesling than on Prosecco, where P. finitimus was more abundant. At the end of the experiments, K. aberrans displaced P. finitimus on both varieties.
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  • 58
    ISSN: 1572-9702
    Keywords: Citrus ; biological control ; Phytoseiidae ; Stigmaeidae ; pesticide disruption ; Thysanoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A series of experiments was initiated on ‘Tahiti’ lime trees to assess possible involvement in yield reduction by one or more species of thrips, especially Frankliniella bispinosa (Morgan) and F. kelliae Sakimura (Thysanoptera: Thripidae). Different pesticides were applied to ensure adequate coverage on flowers and to maintain effective thrips control. Outbreaks of spider mites were anticipated because of the pesticides used and frequency of application. Therefore, population dynamics of the citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae) and the predacious mites Typhlodromalus peregrinus (Muma) (Acari: Phytoseiidae) and Agistemus floridanus Gonzalez (Acari: Stigmaeidae) were monitored in four pesticide treatment regimes during flowering on ‘Tahiti’ lime, Citrus latifolia Tanaka in south Florida. Yield was significantly reduced in four pesticide treatment regimes by 41, 31, 28 and 17% relative to the untreated control. Elimination of predacious mites by the selected pesticides resulted in higher sustained populations of P. citri on treated trees compared to those untreated. The pesticide regimes resulted in differences in population densities of both citrus red mite and its key predators. Depending upon the treatment regime and application date(s), the duration and magnitude of disruption resulted in significant differences in yield. P. citri densities that averaged 5.2 or more per leaf during January–February, or 4.3 or more per leaf during January–March, or 3.9 or more per leaf during January– April contributed substantially to the feeding injury and resulted in reduced yield. Typhlodromalus peregrinus was the most common predacious mite collected during this study (80.4%) followed by A. floridanus with 15.7%. This study demonstrated that the use of one or more pesticides on ‘Tahiti’ lime trees can negatively impact populations of citrus red mites and their associated predators to the detriment of yield. The magnitude of impact was dependent upon the treatment regime and application date(s). Spider mites increased because of reduced predator populations and use of these pesticides, especially in combination, is to be discouraged.
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  • 59
    ISSN: 1572-9702
    Keywords: Phytoseiidae ; walking speed ; biological control ; tritrophic interactions ; Gerbera jamesonii ; trichomes.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of leaf hairs on searching efficiency of adult female Phytoseiulus persimilis was investigated. For this purpose we used the ornamental crop Gerbera jamesonii and determined the predator's searching efficiency on three cultivars that differ largely in the density of leaf hairs on the undersurface of the leaves. Walking speed of the mites was highest on the cultivar with the lowest leaf hair density. Walking activity, defined as the percentage of time spent walking, was not dependent on leaf hair density of the cultivars. At both prey densities tested, time until first predation increased with leaf hair density. The predation rate of adult female P. persimilis is affected by trichome density, particularly when prey density is low. At prey densities of 1.3 and 2.5 Tetranychus urticae eggs cm–2, predation rate was inversely related to leaf hair density. At a prey density of 8.0 eggs cm–2 no significant effect of leaf hair density on predation rate was found. These negative effects on searching efficiency and predation success at low prey density of P. persimilis suggest that biological control of T. urticae on gerbera may be hampered by leaf hairs. © Rapid Science Ltd. 1998
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  • 60
    ISSN: 1572-9702
    Keywords: release ; establishment ; Amblyseius fallacis ; biological control ; Panonychus ulmi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We introduced a mass-reared pyrethroid-resistant strain of the predatory phytoseiid mite Amblyseius fallacis (Garman) into an Ontario peach orchard in an attempt to control populations of the phytophagous mites Panonychus ulmi Koch and Tetranychus urticae Koch (Acari: Tetranychidae). Releases of 1,000 and 2,000 mites per tree were made, at three different times. The release of 2,000 mites per tree in June and in July resulted in significantly higher phytoseiid densities than was observed on control trees. However, densities of P. ulmi or T. urticae were not significantly affected by any release rate or by timing. The release of 1,000 A. fallacis per tree, or of any density in August, did not significantly increase phytoseiid abundance. In the following year, population dynamics of both phytoseiid and phytophagous mites were not significantly affected by the previous year's release. Amblyseius fallacis can be a useful predator in some fruit orchards. However, further research is necessary into the timing and rate of release, modified spray programmes, and with different crops, in order to clarify the role of this species for biological control in Ontario peach orchards.
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  • 61
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    Experimental and applied acarology 23 (1999), S. 97-118 
    ISSN: 1572-9702
    Keywords: Chilocorus ; Coccinellidae ; Exochomus ; Hemisarcoptes ; ultrastructure ; phoresy ; biological control ; parasitism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The non-phoretic stages of mites of the genus Hemisarcoptes are predators of the family Diaspididae. The heteromorphic deutonymph (hypopus) maintains a stenoxenic relationship with beetles of the genus Chilocorus. The mites attach to the subelytral surface of the beetle elytron during transport. There is variation in mite density among species of Chilocorus. Both Hemisarcoptes and Chilocorus have been applied to biological control programmes around the world. The objective of this study was to determine whether subelytral ultrastructure (spine density) plays a role in the evolution of symbiosis between the mite and the beetle. The subelytral surfaces of 19 species of Chilocorus and 16 species of Exochomus were examined. Spine density was determined for five subelytral zones: the anterior pronotal margin, medial central region, caudoventral tip, lateral distal margin and epipleural region. Spine density on the subelytral surface of Chilocorus and Exochomus was inversely correlated with the size of the elytron for all zones except the caudoventral tip. This suggests that an increase in body size resulted in a redistribution of spines and not an addition of spines. The pattern of spine density in Exochomus and Chilocorus follows a single size–density trajectory. The pattern of subelytral ultrastructure is not strictly consistent with either beetle phylogeny or beetle allometry. The absence of spines is not correlated with either beetle genus or size and species of either Chilocorus or Exochomus may be devoid of spines in any zone, irrespective of body size. A general difference between species of Chilocorus and Exochomus is the fact that while spine density in Chilocorus is clinal relative to the size gradient, Exochomus is dichotomous and likely to have either many spines or no spines in a particular zone. No species of Chilocorus was completely devoid of spines. Five species of Exochomus had no spines at all, thus making it difficult to interpret the primary function of the subelytral spines in a general way. Within the genus Chilocorus, spine density may play a synergistic role in host association. Based on morphological evidence alone, these findings lead to the hypothesis that the species of Chilocorus that would be most conducive to biological control application in conjunction with Hemisarcoptes would be Chilocorus cacti, Chilocorus distigma, Chilocorus fraternus, Chilocorus orbus, Chilocorus tristis and, to a lesser extent, Chilocorus bipustulatus. © Rapid Science Ltd. 1998
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  • 62
    ISSN: 1572-9702
    Keywords: biological control ; strawberry ; Tetranychus urticae ; Amblyseius californicus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Naturally occurring beneficials, such as the phytoseiid mite Amblyseius californicus McGregor and the insects Stethorus punctillum Weise, Conwentzia psociformis (Curtis) and others, controlled Tetranychus urticae Koch in 11 strawberry plots near Valencia, Spain, during 1989–1992. The population levels of spider mites in 17 subplots under biological control were low or moderate, usually below 3000 mite days and similar to seven subplots with chemical control. In most of the crops A. californicus was the main predator, acting either alone or together with other beneficials. Predaceous insects colonized the crop when tetranychids reached medium to high levels. For levels above one spider mite per leaflet, a ratio of one A. californicus per five to ten T. urticae resulted in a decline of the prey population in the following sample (1–2 weeks later). These results suggest that naturally occurring predators are able to control spider mites and maintain them below damaging levels in strawberry crops from the Valencia area.
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  • 63
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    Hydrobiologia 403 (1999), S. 13-26 
    ISSN: 1573-5117
    Keywords: Cobitidae ; Cobitis ; taxonomy ; new subgenus ; Turkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the rivers and lakes of Turkey, ten species of loaches of the genus Cobitis occur, viz. C. fahireae, C. varderensis, C. splendens, C. kellei, C. puncticulata, C. strumicae, C. levantina, C. turcica, C. simplicispina and C. bilseli. Cobitis bilseli is separated into a new subgenus, Beyshehiria.
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  • 64
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    Keywords: Macrothrix laticornis-complex ; morphology ; taxonomy ; distribution ; males
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    Notes: Abstract Macrothix laticornis is redescribed and compared with populations from central North America (Mexico), and from south-east Asia (Vietnam). All three are closely related, but with sufficient morphological differences to establish a laticornis species-group. Two species new for science are described, viz. M. sierrafriatensis (Mexico) and M. vietnamensis (Vietnam).
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  • 65
    ISSN: 1573-5117
    Keywords: Branchiopoda ; ‘Cladocera’ ; Anomopoda ; Macrothrix tripectinata ; morphology ; redescription ; taxonomy ; key ; Eurasia ; distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rare Macrothrix tripectinata Weisig, 1934 is redescribed on material from the East Pamirs (Tajikistan) and Tuva (Russia). Adult and juvenile parthenogenetic females and adult males were studied using optical microscopy and SEM. The thoracic appendages and the molar surface of mandibles were investigated in detail. Selected ‘conventional’ diagnostic features are critically reviewed. The importance of characters, rarely used in the systematics of Macrothrix, such as: type of valve reticulation, similarity or dissimilarity in size of the terminal esthetascs of the antennules of females and males, presence of an abdominal projection and reticulation on the postabdomen, presence, length and armature of a male seta on the antennule, are discussed. A key for the Eurasian species of Macrothrix is proposed.
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  • 66
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    Hydrobiologia 403 (1999), S. 87-95 
    ISSN: 1573-5117
    Keywords: Thermocyclops ; China ; taxonomy ; distribution
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    Topics: Biology
    Notes: Abstract Based on the result of investigations and former records, there are five freshwater species of the genus Thermocyclops in China, namely T. taihokuensis Harada, 1931, T. crassus (Fischer, 1853), T. vermifer Lindberg, 1935, T. dybowskii (Lande, 1890), and T. kawamurai Kikuchi, 1940. Two previously recorded species, T. brevifurcatus Harada, 1931 and T. hyalinus (Rehberg, 1880) are recognized as synonyms of Thermocyclops crassus. Another species, Thermocyclops mongolicus Kiefer, 1937, described from China by Tai & Chen, is a synonym of T. taihokuensis based on this study. Thermocyclops orientalis Dussart & Fernando, 1985 was compared with Thermocyclops kawamurai Kikuchi, 1940. It shows conspicuous differences and it seems unlikely that it is a synonym of T. kawamurai. The paper presents diagnostic characteristics of each species and a key to five Chinese species of Thermocyclops.
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  • 67
    ISSN: 1573-5117
    Keywords: aquatic Oligochaeta ; Lake Biwa ; Japan ; taxonomy ; zoogeography
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    Topics: Biology
    Notes: Abstract At least 41 taxa of aquatic oligochaetes including five new records have been recorded from the ancient Lake Biwa in Japan. The lake is characterized by eurybathyal occurrence of Branchiura sowerbyi. The posterior gill filaments of B. sowerbyi develop normally in lagoons adjacent to the lake, while in the lake they weakly develop or often disappear at littoral and sublittoral depths shallower than 30 m, and they almost completely disappear in the profundal zone. The profundal population of B. sowerbyi also differs from other populations in eastern Asia in having fewer and shorter hairs, and simple-pointed crotchets, or bifid crotchets with parallel teeth, in dorsal bundles of chaetae. This suggests that an intralacustrine speciation is progressing in the profundal population. An aberrant modification is found in the pectination of dorsal crotchets in Tubifex tubifex occurring at deep profundal depths of the lake. The ventral chaetae in segment II of Teneridrilus mastix are rarely modified in Lake Biwa. This requires cancellation of the modification of chaetae in II as a diagnostic characteristic of the genus Teneridrilus. Limnodrilus amblysetus, previously known only from China, is recorded from Lake Biwa. Descriptions and taxonomic remarks are provided for Haemonais waldvogeli (new to Japan), Pristina longiseta, Stephensoniana trivandrana and Ripistes parasita (new to Lake Biwa), and Ophidonais serpentina, based on Lake Biwa material.
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  • 68
    ISSN: 1573-5117
    Keywords: Myriophyllum aquaticum ; Lysathia n. sp. ; Chrysomelidae ; biological control
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    Topics: Biology
    Notes: Abstract The plant, Myriophyllum aquaticum (Vell.) Verdc. (parrot's feather), is one of five alien water weeds causing problems in South Africa. M. aquaticum is presently considered a minor weed problem, but it is expected that it will become more important as the more troublesome water weeds are brought under control. For this reason, research was initiated on the possibility of biological control of M. aquaticum. Exploratory surveys on M. aquaticum in South America, the continent of origin, yielded several promising insect natural enemies. A leaf-feeding beetle, Lysathia n. sp. (Chrysomelidae), was the first biological control candidate imported. Its biology was studied and under quarantine conditions it was found that Lysathia n. sp. was host-specific, feeding and reproducing only on M. aquaticum. Permission for release was sought and given by the National Department of Agriculture, Directorate of Plant and Quality Control. This insect was released at five sites throughout the country in December 1995. Post-release studies on this beetle have been in progress for three years. An account follows of its field performance at one of these sites: Culmpine, Vaalwater, Northern Province on the Mokolo River. The results obtained at the Culmpine site indicate that Lysathia n. sp. shows promise as a biological control agent. A second agent may however be required at a later stage.
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  • 69
    ISSN: 1573-5117
    Keywords: actinomycetes ; biological control ; bluegreen algae ; cyanobacteria ; protozoa ; Streptomyces
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    Topics: Biology
    Notes: Abstract A range of naturally occurring organisms are available for the biological control of cyanobacteria: including viruses, bacteria, fungi, actinomycetes and protozoa. Development of these organisms as biological control agents involves isolation from environmental samples, characterisation of anti-cyanobacterial activity, microcosm and large-scale field experiments and final development of a biological control lake management strategy. Two groups of antagonist are considered in detail – actinomycetes (e.g. Streptomyces exfoliatus, mode of action by production of a lytic agent) and protozoa (Nuclearia delicatula and Nassula tumida, mode of action by predation). The effectiveness of biological control agents in the lake environment depends on a range of biological and physico-chemical factors. Various strategies can be implemented to optimise their activity.
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  • 70
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    Hydrobiologia 398-399 (1999), S. 65-74 
    ISSN: 1573-5117
    Keywords: Australia ; Coral Sea ; marine algae ; taxonomy ; biogeography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The marine benthic algae from North East Herald Cay, Coral Sea, South Pacific, are listed with taxonomic, bibliographic and biogeographic details. The checklist includes 66 species of which 23 are green, 2 are brown, and 41 are red algal species. The almost complete absence of brown algae from what is seemingly a typical tropical reef environment on which a true coral cay has developed is noteworthy. All samples were from the lagoon, which forms the concave side of a crescent-shaped reef and which ranges in depth from 0–30 m. The endemic Caribbean green alga Chamaedoris peniculum is recorded for the Pacific for the first time, and a possibly undescribed species of the genus Rhipiliopsis was also discovered. Although only preliminary, the survey shows that the marine flora is seemingly typical of coral cays for the general region of the Great Barrier Reef.
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  • 71
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    Hydrobiologia 406 (1999), S. 9-19 
    ISSN: 1573-5117
    Keywords: Annelida ; Clitellata ; history ; taxonomy ; ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fourteen aquatic oligochaetes were described between 1773 and 1828. Dramatic increases in descriptions occurred in the decades beginning 1900, 1930 and 1960. These can be associated with specific authors, such as W. Michaelsen and S. Hrabě. In the period 1970–1990, some 250 marine species (excluding Enchytraeidae) were described, largely by C. Erséus. Some of the major scholars, such as W. Michaelsen and F. Vejdovský, I. I. Malevič and P. G. Svetlov, founded centers of oligochaete study that survive in Europe today. Others, such as those in the English speaking world, have had to develop in isolation. While numerical and phenetic methods had little influence on microdrile systematics, phylogenetic (parsimony) methods have, since the first publication by B. G. M. Jamieson. Most literature prior to 1970 was taxonomic. Other earlier work documented community structure in relation to pollution. The study of Rybinsk Reservoir (Russia) stimulated a large body of ecological and biological work by T. L. Poddubnaya. The St. Lawrence Great Lakes was also a focus for worm studies until recently. Early physiological work focused on respiration, with studies dating from the 1920s. This has been continued in relation to pollution ecology and energy flow. Physiological work was notably absent from our first proceedings volume and only poorly represented since then. Future prospects are difficult to identify. Developments in ultrastructure (including sperm and muscle cells), molecular biology and parsimony or cladistic phylogenetic methods will move from rather simple initial work that shows the possibilities, to a revisionary phase where the complexities begin to be revealed before it is possible to assess their value. This author has too superficial a level of understanding to predict the outcome, but suspects that agreement among the results of several methods is needed for them to be persuasive. I would hope that multivariate methods of data analysis in pollution studies would prove superior to the development of indices and over-reliance on toxicology. Toxicity studies are of use in comparing risks among products but not in predicting field effects. Benthic biology needs a method for identifying factors that are directly related to worm biology, such as food, rather than physical and chemical factors related to the water column. Surveys, relating communities to chemical and physiographic factors will remain unpredictive. It is easier to forsee some changes in taxonomy that are almost overdue. The haplotaxids should be broken up into several small families, Haplotaxis s.s. will be one of them, with a number of highly adapted and very similar species, many currently regarded as synonymous, redefined by careful anatomical study. The lumbriculids and branchiobdellidans may well be combined into a single taxon defined by their unique semi-prosopore male ducts, with lumbriculids defined by their unique muscle structure and the branchiobdellids by many apomorphic characters related to ectocommensal life. The position of Hirudinea remains unresolved, but relationship to this taxon is not excluded. Detailed study of penes in lumbriculids is required. The naidids and tubificids seem to be a monophyly. Existing genera may be hard to analyze phylogenetically because definitions are based on atrial form, which is hard to express as characters. Chaetotaxy in Naididae will prove insufficient and many synonyms will be declared. Capilloventridae and Randiellidae require description of male ducts and gonads. They may provide evidence counter to the octogonadal theory of oligochaete descent, or of polyphyly in the group. Knowledge of phreodrilid diversity will increase. Work will increase in Asia and South America, but sub-Saharan Africa remains under explored. Fish diseases will create more interest in North American worms. New methods, including molecular studies, and renewed interest in ontogeny and detailed anatomical studies, may improve our ability to resolve clusters of taxa with few, if any, apomorphic character states that remain at the base of cladograms. An ability to include sound evidence from vestigial organs and logical arguments on a 'weight of evidence' basis is needed as an adjunct. While molecular studies seem to hold much promise, early studies can prove over simplistic and can provide conflicting hypotheses. We need to complete the review of taxonomy according to parsimony before major terminology changes, such as abandonment of the term Oligochaeta, can gain acceptance.
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  • 72
    ISSN: 1573-5117
    Keywords: mitochondrial DNA ; cytochrome oxidase I ; competitive PCR ; copepoda ; taxonomy ; marine zooplankton
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    Topics: Biology
    Notes: Abstract Accurate taxonomic identification of species at all life stages is critical to understand and predict the processes that together determine marine community dynamics. However, zooplankton assemblages may include numerous sibling and congeneric species distinguished by subtle morphological characteristics. Molecular systematic databases, including DNA sequences of homologous gene regions for selected taxonomic groups, allow the design of rapid protocols to determine species' diversity and identify individuals. In this study, the DNA sequence of a 300 base-pair region of the mitochondrial cytochrome oxidase I (COI) gene was determined for eight species of three genera of calanoid copepods: Calanus finmarchicus, C. glacialis and C. helgolandicus; Neocalanus cristatus, N. flemingeri and N. plumchrus; and Pseudocalanus moultoni and P. newmani. The DNA sequences differed between congeneric species by 13 – 22% of the nucleotides; the protein sequences differed by zero to five amino acid substitutions. Both the DNA and amino acid sequences resolved the evolutionary relationships among congeneric species; relationships among the genera were not well-resolved by this region of mtCOI. Using the same conserved primers, the only amplification product for C. finmarchicus was an aberrant sequence (and putative pseudogene) which differed from the C. finmarchicus COI sequence by 36% of the nucleotides and 32 amino acid substitutions. Species-specific oligonucleotide primers were designed for Calanus spp. (which cannot be distinguished at larval stages) and Pseudocalanus spp. (which are difficult to distinguish even as adults). Individual copepods were identified using competitive, multiplexed species-specific polymerase chain reactions (PCR) in two studies of co-occurring sibling species. The first study confirmed the presence of three Calanus spp. in Oslofjord, Norway and found a predominance of C. helgolandicus. The second study determined patterns of distribution and abundance of Pseudocalanus spp. on Georges Bank in the NW Atlantic and showed that P. moultoni predominated in shallow and coastal waters, while P. newmani was more abundant in offshore regions flanking the Bank. Competitive, species-specific PCR is a useful tool for biological oceanographers. This simple, rapid, and inexpensive assay may be used to identify morphologically-similar individuals of any size and life stage, and to determine a species' presence or absence in pooled samples.
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  • 73
    ISSN: 1573-5117
    Keywords: grass carp ; submerged weeds ; biological control ; channels ; stocking rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two experiments were conducted in Río Negro province (Argentina) to study macrophyte response to different stocking rates of the grass carp (Ctenopharyngodon idella Val.). The first of these was performed in nine polyethylene-lined ponds, 12 m2 each, planted with Illinois pondweed (Potamogeton illinoensis Morong) at two fish stocking rates, 100 and 360 kg ha-1. The second experiment was set up under natural conditions in a channel full of Potamogeton pectinatusL., Myriophyllum aquaticum (Vell.) Verd. and Chara contrariaA. Braun ex Kurtz, at 100 and 200 kg ha-1. In both cases, the initial individual fish weight was between 22 and 40 g. A complete randomised block design was used in both experiments. Five samples of plant biomass were taken per experimental unit. Data were subjected to ANOVA and LSD was used as a comparative test. In both cases, the experimental period was 2 months because of the herbivory under the high stocking rate completely depleted both the leaves of P. illinoensis under pond conditions and the aboveground plant biomass in the channel. The results showed a high potential of biocontrol under tested conditions and suggest a stocking rate lower than 100 kg ha-1 as enough for local conditions and for a prolonged fish presence in the area.
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  • 74
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    Hydrobiologia 415 (1999), S. 203-206 
    ISSN: 1573-5117
    Keywords: Red water fern ; biological control ; mechanical control ; herbicide control ; utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Azolla filiculoides Lamarck (Pteridophyta: Azollaceae) (red water fern) is native to South America and was first recorded in South Africa in 1948. The lack of natural enemies and the presence of enriched waters have contributed to its establishment throughout the country where dense infestations cause severe degradation of aquatic ecosystems and limit their utilisation. The biology of A. filiculoides precludes it from being controlled manually or mechanically and herbicidal control in the aquatic environment is undesirable. Biological control is suggested as the only sustainable control method for this weed and a natural enemy, Stenopelmus rufinasus Gyllenhal (Coleptera: Curculionidae), was released for this purpose in late 1997. The weevil has established well and has caused a significant decline in the Azolla mat at a number of sites, suggesting that the weevil will be an efficient tool in the management of A. filiculoides in South Africa.
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    Reviews in fish biology and fisheries 9 (1999), S. 325-352 
    ISSN: 1573-5184
    Keywords: evolution ; nomenclature ; phylogeny ; species ; systematics ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract It is argued, with selected examples from freshwaterfish systematics, that species should be viewed as anexpression of self-perpetuated clustered variation innature, conforming to the phylogenetic speciesconcept. The importance of species lies in thefunctional and structural significance of theirdiagnostic characters. Species can be nested by theircharacters into a tree diagram (phylogeny) orhierarchical alignment structure (classification) ofcharacter distribution, which may be taken to reflectevolution, the unifying theory of organismaldiversification. The phylogenetic species concept,which emphasizes recognition of a pattern ofvariation, describes better than any other proposedconcept the units called species by systematists.Other concepts are based on processes and normally donot permit recognition of particular taxa. Specieshave unique histories, and speciation may proceed bydifferent mechanisms. Whereas it may be postulatedthat speciation entails an irreversible change in thegenetic structure of taxa, recognized by phenotypicexpression and apparently also maintained to a largeextent by selection for a particular phenotype,species recognition must remain independent ofassumptions about species history and spatialdistribution. Species are monophyletic taxa and thespecies category does not differ significantly inphylogenetic regard from other systematic categories.Species as such are not necessarily evolutionaryunits. It is recommended to apply species names withreference to the diagnostic characters of the speciesand to abandon the type specimen described by theInternational Code of Zoological Nomenclature as anomenclatural reference unit.
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  • 76
    ISSN: 1573-9708
    Keywords: biostratigraphy ; Fiji ; Gastropoda Indonesia ; Miocene ; Mollusca ; Pteropoda ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Pteropoda (Mollusca, Gastropoda, Euthecosomata) described by Beets (1943, 1950, 1953) from the alleged Late Miocene/Early Pliocene asphaltic deposits of Buton (SE Sulawesi, Indonesia) are revised. The following species are distinguished: Styliola subula (Quoy & Gaimard, 1827), Cavolinia bituminata (Beets, 1953), Cavolinia mexicana (Collins, 1934), Cavolinia vendryesiana (Guppy, 1873) and Diacria mbaensis Ladd, 1934. A comparison with the Fiji pteropod associations suggests a Miocene (Tortonian to Messinian) age for the Indonesian faunules. Pteropod species described by Ladd (1934) from Viti Levu (Fiji Islands), are revised in an appendix. Five species are distinguished, viz. Creseis acicula (Rang, 1828) (?), Cavolinia gypsorum (Bellardi, 1873), C. mexicana, C. rewaensis (Ladd, 1934) and Diacria mbaensis Ladd, 1934. The presence of Cavolina gypsorum dates this association as Miocene (Tortonian–Messinian).
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  • 77
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    Netherlands journal of geosciences 78 (1999), S. 215-229 
    ISSN: 1573-9708
    Keywords: Actinopterygii ; Coccolepidae ; Halecomorphi ; Halecostomi ; taxonomy ; Teleostei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract An overview is presented of the actinopterygian fishes from the Late Jurassic (Late Kimmeridgian and Early Tithonian) ‘Plattenkalke’ near Solnhofen (Solnhofen lithographic limestone), Bavaria, Germany. The fish fauna is very diverse, with the palaeonisciform Coccolepis, halecostomes such as Lepidotes, Heterostrophus, three genera of macrosemiids and six genera of pycnodontiforms, halecomorphs including two genera of caturids, two genera of amiids, Ophiopsis, ‘Furo’, Ionoscopus, Brachyichthys, Callopterus, Liodesmus, ?Lophiurus, five genera of pachycormids, three genera of pholidophorids, Pleuropholis, two genera of aspidorhynchids and eleven genera of basal teleosts. Although several groups have been subject of revision, most of the fauna remains poorly known. Study of this rich fauna will provide a lot of information on the phylogeny and interrelationships of halecostome fishes.
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    Netherlands journal of geosciences 78 (1999), S. 267-280 
    ISSN: 1573-9708
    Keywords: ‘Agnatha’ ; biostratigraphy ; Gnathostomata ; Ludlovian ; Pridolian ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract The fauna from a Late Silurian residue sample from Övedskloster (Skåne) is listed. It comprises scales of ‘Agnatha’ (rare osteostracan scales, and the thelodonts Thelodus parvidens, ‘T. traquairi’, T. sculptilis, T. admirabilis, and ‘Loganellia cuneata’) and of Gnathostomata (the acanthodians Nostolepis striata, Gomphonchus sandelensis, Poracanthodes? lehmani sp. nov., and Poracanthodes cf. punctatus, tesserae of the head and other specialised elements of the squamation; and the osteichthyan Andreolepis hedei). This fauna is considered to be as old as, or slightly younger than that from the complex of sedimentary rocks along the Anglo-Central Urals margin of the Laurussian continent affected by the Ludfordian A. hedei Event. This event marks the transition between the hedei and sculptilis Zones of the Microvertebrate Standard Zonation and can be correlated with the snajdri Conodont Interval Zone. The evidence favours a simultaneous palaeogeographical appearance of the punctatiform and porosiform poracanthodid acanthodians within the Baltica region of Laurussia.
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    World journal of microbiology and biotechnology 15 (1999), S. 535-543 
    ISSN: 1573-0972
    Keywords: Biochemical ; biological control ; molecular ; rhizobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The rhizosphere is the narrow zone of soil surrounding the root that is subject to influence by the root. Rhizobacteria are plant-associated bacteria that are able to colonize and persist on roots. An understanding of the ecology of a microorganism is a fundamental requirement for the introduction of a microbial inoculant into the open environment. This is particularly true for biological control of root pathogens in the rhizosphere, where one is actively seeking to alter the ecological balance so as to favour growth of the host plant and to curtail the development of pathogens. Some strains of plant growth-promoting rhizobacteria can effectively colonize plant roots and protect plants from diseases caused by a variety of root pathogens and growth promotion of plants through direct stimulation of growth hormone. Such beneficial or plant health-promoting strains are emerging as promising biocontrol agents. They are suitable as soil inoculants either individually or in combination and may be compatible with current chemical pesticides. Considerable progress has been achieved using molecular genetic techniques to elucidate the important microbial factors or genetic traits involved in the suppression of fungal root diseases. Strategies utilizing molecular genetic techniques have been developed to complement the ongoing research ranging from the characterization and genetic improvement of a selected biocontrol agent to the measurement of its persistence and dispersal. Finally, biocontrol is considered as part of a disease control strategy like integrated pest management which offers a successful approach for the deployment of both agro-chemicals and biocontrol agents.
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  • 80
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    Interface science 7 (1999), S. 251-271 
    ISSN: 1573-2746
    Keywords: triple junctions ; taxonomy ; dimensionality ; structure ; energy ; segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We provide an overview of the properties of triple junctions and quadruple points. It is shown that these junctions may exhibit distinct behaviors that imply that they have and thermodynamically distinct properties in the same way that grain boundaries can be considered as thermodynamically distinct phases, separate from the material that they inhabit. It is shown that the treatment of triple junctions as thermodynamically distinct defects is a natural extension of the treatment of grain boundaries, and that it can be further extended to other junctions such as quadruple nodes. Equilibrium dihedral angles under conditions of anisotropic interfacial energy are explored, and it is found that the dihedral angles may be variable under a range of different conditions.
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  • 81
    ISSN: 1573-8469
    Keywords: Alternaria brassicae ; biological control ; cross infection ; disease management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Resistance in susceptible mustard cv. PR-15 against the highly virulent A. brassicae isolate A (AbA) and moderately virulent isolate C (AbC) was induced using an avirulent Alternaria brassicae isolate D (AbD). The induction of resistance due to AbD against AbA or AbC resulted in significant reduction in disease severity. The A. alternata (Aa) failed to induce resistance against AbA and AbC, on the contrary it induced susceptibility against them.
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  • 82
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    European journal of plant pathology 105 (1999), S. 177-189 
    ISSN: 1573-8469
    Keywords: biological control ; grey mould ; mechanisms ; pathogenicity enzymes ; proteolysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The role of protease of Trichoderma harzianum in the biocontrol of Botrytis cinerea was examined. Two isolates of T. harzianum were compared for their ability to produce protease in liquid culture medium and on the surface of bean leaves. The biocontrol agent T. harziaum T39 produced 58 mU/ml of protease and T. harzianum NCIM1185 produced 54 mU/ml on the 5th day of growth in liquid culture medium. On bean leaves, combinations of B. cinerea and T. harzianum isolates were examined for the synthesis of protease. The protease activities were 0.9 and 0.6 mU/ml for T. harzianum T39 and NCIM1185, respectively, and 0.5 mU/ml for B. cinerea alone after 48 h of incubation. In the presence of T. harzianum T39 culture liquid containing protease, a 55% reduction in B. cinerea germination and a 80% reduction in the germ tube length were observed after 17 h of incubation in vitro. When T. harzianum isolates were added to B. cinerea on bean leaves, increased synthesis of protease was observed (1.0 and 1.2 mU/ml for T39 and NCIM1185, respectively). In the presence of T. harzianum NCIM1185 protease, although the rate of germination was reduced, B. cinerea attained 98% germination after 17 h of incubation. The hydrolytic enzymes produced by B. cinerea, endo-polygalacturonase (PG) and exoPG were partially deactivated by protease from the T. harzianum isolates. Carboxymethyl cellulase was deactivated only by protease of NCIM1185. On the surface of bean leaves, the protease (obtained from liquid culture medium of T. harzianum isolates) resulted in 56–100% reduction of disease severity. The culture liquid containing protease synthesized on the surface of bean leaves treated with B. cinerea and with T. harzianum was collected and added to fresh leaves infected by B. cinerea. There was 56–100% and 30–75% reduction of disease severity with liquid droplet collected from the leaves treated with T. harzianum T39 and NCIM1185, respectively. Increased control of disease was obtained by combining the conidia of T. harzianum isolates with protease obtained from culture media. Protease inhibitors, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E64), antipain hydrochloride, and a mixture of inhibitors, but not pepstatin A, fully or partially nullified the biocontrol effect of T39. T39 was found to be a poor producer of chitinase and β-1,3-glucanase in vitro. These enzymes were not detected on leaves treated with T39. Involvement of protease in biocontrol of B. cinerea is suggested.
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  • 83
    ISSN: 1573-8248
    Keywords: Acari ; biological control ; generalist ; intraspecific predation ; multiple species release ; predatory mites ; specialist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We examined intra- and interspecific predation of adult females and immature stages of the generalist Neoseiulus californicus and the specialist Phytoseiulus persimilis. Adult females and immatures of both predators exhibited higher predation rates on larvae than on eggs and protonymphs. N. californicus fed more inter- than intraspecifically. Predation on P. persimilis by N. californicus was more severe than vice versa. P. persimilis had higher predation rates on conspecifics than heterospecifics and was more prone to cannibalism than N. californicus. When provided with phytoseiid prey, P. persimilis suffered higher mortality than N. californicus. When held without food, adult females and protonymphs of N. californicus survived longer than the corresponding stages of P. persimilis. N. californicus females were able to sustain oviposition when preying upon P. persimilis, whereas cannibalizing females did not lay eggs. Females of P. persimilis were not able to sustain oviposition, irrespective of con- or heterospecific prey. Immatures of both predators were able to reach adulthood when provided with either con- or heterospecifics. Juvenile development of N. californicus was shorter with heterospecific vs. conspecific larvae; mortality of P. persimilis immatures was less when feeding on conspecific vs. heterospecific larvae. Different behavioral pattern in intra- and interspecific predation are discussed in regard to their feeding types (generalist vs. specialist).
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  • 84
    ISSN: 1573-8248
    Keywords: biological control ; Botrytis aclada ; Botrytis cinerea ; cyclamen ; ecological adaptation ; hydrangea ; lily ; onion ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ulocladium atrum and Gliocladium roseum are fungal antagonists capable of suppressing sporulation of Botrytis spp. on dead plant parts. The effect of temperature (3 to 36 °C) on antagonist conidial germination and mycelial growth was assessed on agar. In addition conidial germination of U. atrum was measured on dead lily leaves. The optimum temperature of both antagonists for both conidial germination and mycelial growth was between 27 and 30 °C. U. atrum was less affected by lower temperatures than G. roseum. At optimum temperature, 50% of conidia of U. atrum and G. roseum germinated within 2.6 and 10.0 hrs, respectively. At low sub-optimal temperatures (6 °C), 50% of conidia germinated within 18 and 96 hours, respectively. In bioassays on dead onion leaves, U. atrum suppressed sporulation of B. cinerea and B. aclada at all temperatures tested (6 to 24 °C) by more than 85%. On dead cyclamen leaves, G. roseum was more efficient than U. atrum at 21 and 24 °C but, in contrast to U. atrum, showed no antagonistic activity at temperatures below 21 °C. On dead hydrangea leaves, U. atrum significantly reduced sporulation of B. cinerea at temperatures as low as 3 and 1 °C. Under Dutch growing conditions, the mean air temperature during leaf wetness periods in onion and lily fields was 15 °C with temperatures only occasionally above 20 °C. In greenhouse crops of cyclamen, the mean temperature during high humidity periods was 17 °C. It is therefore concluded that U. atrum is better adapted than G. roseum to temperatures which occur in the field, in greenhouse crops such as cyclamen, or during cold storage of plant stocks.
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  • 85
    ISSN: 1573-8248
    Keywords: Anaxipha longipennis ; biological control ; Gryllidae ; Metioche vitatticollis ; parasitism ; predation ; Trichogramma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Egg mortality of rice leaf folders Cnaphalocrocis medinalisand Marasmia patnalis was studied in unsprayed irrigated rice fields in Laguna Province, the Philippines. Mortality was assessed by field exposure of laboratory-laid eggs for two days and by monitoring of field-laid eggs. Egg disappearance, the major mortality factor, was low in the first four weeks after transplanting and then increased. Egg parasitism by Trichogrammajaponicum was highest at the start of the crop and decreased to a low level towards crop maturity. Non-hatching of eggs was of minor importance. Over the total duration of the egg stage, the average disappearance of exposed laboratory-laid eggs was40%, and of field-laid eggs 46%. Egg mortality due to parasitism averaged 15% and 18%, respectively. The potential impact of egg parasitism is probably partly obscured by the disappearance of parasitized eggs. Mortality rates were highly variable between egg cohorts, but with multiple regression analysis several factors were identified that statistically explained a significant part of this variation. The results suggest that the predatory crickets Metiochevittaticollis and Anaxipha longipennis play a major role in egg disappearance, and that egg parasitism is positively dependent on the overall density of host eggs of Trichogramma in the field.
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  • 86
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    BioControl 44 (1999), S. 159-169 
    ISSN: 1573-8248
    Keywords: biological control ; Euphorinae ; species identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four parasitoids of the subfamily Euphorinae are common in New Zealand pasture. These are Microctonus aethiopoides, M. hyperodae, M. zealandicus and Dinocampus coccinellae. There is overlap in these parasitoids' host ranges and, although adults of these species can be identified morphologically, their larval stages have not yet been compared. This contribution provides morphological descriptions of the head capsules of 1st instar larvae that enable the four parasitoid species to be distinguished. Diagnostic characteristics are the shape of the mandible and the pattern of sclerotisation on the hypopharynx. Post 1st instar larvae can be identified by retrieving the shed 1st instar head capsule from the host abdomen.
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  • 87
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    BioControl 44 (1999), S. 347-367 
    ISSN: 1573-8248
    Keywords: Aphidiidae ; biological control ; Chrysopidae ; Coccinellidae ; entomopathogens ; Hemerobiidae ; Syrphidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sixteen cohorts of the brown citrus aphid, Toxoptera citricida, were followed from colony initiation to maturation/extinction in citrus groves at two sites in Puerto Rico and nine sites in Florida, USA. Infested citrus terminals were sampled repeatedly in a non-destructive manner and data recorded on the recruitment of natural enemies and the fate of aphid colonies. Coccinellidae were the most efficient predators of T. citricida, primarily Cycloneda sanguinea, and Coelophora inaequalis (Puerto Rico), C. sanguinea, Harmonia axyridis (Florida). Coccinellids were abundant in citrus year-round in Puerto Rico, but in Florida citrus their abundance peaked in spring and declined thereafter. Syrphid flies were other important predators, especially Pseudodorus clavatus (Florida and Puerto Rico) and Ocyptamus fuscipennis (Puerto Rico). Syrphids increased in abundance from spring to fall in both regions. Other predators included Cereaochrysa lineaticornis, Chrysoperla rufilabris (Chrysopidae) and Micromus posticus (Hemerobiidae). The parasitoid Lysiphlebus testaceipes was ubiquitous in T. citricida cohorts, but its impact on colony survival was usually low. The fungal pathogen Verticillium lecanii was a significant source of colony mortality at one site in Puerto Rico, but was not observed on T. citricida in Florida.
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  • 88
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    BioControl 44 (1999), S. 291-299 
    ISSN: 1573-8248
    Keywords: biological control ; cerambycid ; Coleoptera ; pine wood borer ; poplar wood borer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Artificial diets for the colydiid beetle, Dastarcus helophoroides, a predator of cerambycid beetles and xylocopid bees, were evaluated. Hatched larvae were reared on artificial diets composed of silkworm pupa-powder, dry yeasts, yeast extract, sucrose, peptone, squid liver oil, preservatives and distilled water, but their emergence rate was very low. If larvae were fed paralyzed cerambycid larvae till they became approximately 8 mm in body length and then reared on artificial diet, emergence rates were high. Using the latter method, mass-production of this colydiid beetle is feasible.
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  • 89
    ISSN: 1573-8248
    Keywords: bacterial antagonists ; biological control ; Pantoea agglomerans ; pseudosclerotia ; Sclerotiniaceae ; Vaccinium macrocarpon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bacteria inhibitory to Moniliniaoxycocci, the cranberry cotton ball pathogen, were identified. Eighty-three bacteria isolated from a cranberry marsh and Erwinia herbicola C9-1,which is being developed elsewhere for the biological control of fire blight of pome fruits, were tested fortheir ability to inhibit radial growth and conidial germination of M. oxycocci in vitro. IsolateBA35 from cranberry (tentatively identified as Pseudomonas syringae pv. morsprunorum) and E. herbicola C9-1 completely inhibited radial growth of M. oxycocci. BA35 and C9-1 were among the most effective of 21 bacteria tested for inhibition of conidial germination. Growth of bacteria in sclerotial extracts of M. oxycocci and Sclerotinia sclerotiorum was determined in order to select isolates that might be successful in degradingsclerotia, thereby reducing the viability of M.oxycocci. Populations of isolates 62 and S-18(identities unknown), and S-19 (tentatively identified as Micrococcus luteus), increased approximately3.0--5.0 log10 units in all extracts within 24 h. Populations of isolate S-10 increased by about 3.5log10 units in all sclerotial extracts within 48 h. Populations of isolate S-49 (identity unknown)were lower in extracts of M. oxycocci than S. sclerotiorum after 48-h. In buffer controls, bacterial populations remained stable or decreased over the 48-h period.
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  • 90
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    BioControl 43 (1999), S. 441-456 
    ISSN: 1573-8248
    Keywords: agroecosystems ; biological control ; Carabidae ; carabid community ; Coleoptera ; generalist predators ; natural enemy abundance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Generalist natural enemies such as carabid beetles have the potential to maintain a variety of pests below outbreak levels in annual crops. To assess the relationship between carabid beetle abundance and field rates of prey removal, we created plots surrounded by different boundaries that selectively affected dispersal of edaphic arthropods, primarily carabids. Three treatments were established: (1) naturally occurring communities, (2) augmented communities using ingress boundaries, and (3) reduced communities using egress boundaries. Selective boundaries altered carabid communities with minimal habitat alteration and without use of insecticides. Three times during the growing season, a fixed number of onion fly pupae were placed in plots to evaluate the impact of carabid abundance on predation rates. A combination of vertebrate and invertebrate exclosures allowed us to evaluate prey removal by invertebrates alone. In comparison to the no boundary treatment, carabids increased 54.2% and decreased 83.1% in plots surrounded by ingress and egress boundaries respectively. Predation rates were positively correlated with carabid abundance (r2 = 0.70, p 〈 0.0001). Significantly more pupae were removed from exclosures allowing access to invertebrates alone than from total exclosures, suggesting that invertebrates represented an important group of predators. Laboratory trials tested the feeding potential of the four most abundant carabid species and showed that they readily consumed onion fly pupae, supporting our hypothesis that carabids were the main predators in field tests. This study corroborates and extends previous observations of the importance of carabid beetles as generalist predators of insect pests in agricultural fields.
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  • 91
    ISSN: 1573-8248
    Keywords: Acari ; biological control ; cannibalism ; generalist ; interspecific predation ; multiple predators release ; predatory mites ; specialist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Discrimination between and predation preference for con- or heterospecific larvae was examined for adult females of P. persimilis and N. californicus in plexiglass cages with and without their primary prey T. urticae. Rates of intra- and interspecific predation on larvae were measured for females held on leaves and provided with excess amounts of spider mites. Females of the generalist N. californicus distinguished con- and heterospecific larvae and preferred to prey upon the latter. Females of the specialist P. persimilis appeared to lack discrimination ability and fed equally on con- and heterospecifics. When spider mites and phytoseiids were offered simultaneously, all P. persimilis females chose to first attack T. urticae, whereas N. californicus females attacked both tetranychids and heterospecific phytoseiids. Females of both predators preyed upon phytoseiid larvae when held on leaves with surplus T. urticae: while P. persimilis fed on both con- and heterospecifics, N. californicus attacked larvae of P. persimilis but avoided cannibalizing larvae. The different behaviors of P. persimilis and N. californicus are discussed with regard to different predation types (generalists vs. specialists) and the possible consequences of mixed release for biological control of spider mites in greenhouses.
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  • 92
    ISSN: 1573-8248
    Keywords: biological control ; kaolin ; mycoherbicide ; preservation ; weed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Different solid substrates were investigated as spore production methods for Stagonospora convolvulistrain LA39, a potential bioherbicide for field bindweed (Convolvulusarvensis L.). Up to 4 × 108 spores/g of substratewere yielded on cous-cous (cracked hard wheat). Thespores were as pathogenic as those grown on artificial medium (V-8-juice agar). The air-drying on kaolin and storage at 3 °C kept spores viable and pathogenic for 180 days. Spore germination exceeded70% for the first 140 days and then declined to 50%after 175 days. Less than 5% of spores were still viable after 17 months. The preservation of stock cultures in 10% glycerine at −80 ° C and in liquid nitrogen did not affect viability orpathogenicity of the spores.
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