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  • Animals
  • Inorganic Chemistry
  • fish
  • Springer  (16)
  • 1995-1999  (16)
  • 1950-1954
  • 1996  (16)
Collection
Keywords
Publisher
Years
  • 1995-1999  (16)
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    Evolutionary relationships of eukaryotic kingdoms (1996)
    Springer
    Journal of molecular evolution 42 (1996), S. 183-193 
    Details
    ISSN: 1432-1432
    Keywords: Small-subunit ribosomal RNA ; Phylogeny ; Animals ; Fungi ; Plants ; Alveolates ; Heterokonts ; Stramenopiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The evolutionary relationships of four eukaryotic kingdoms—Animalia, Plantae, Fungi, and Protista—remain unclear. In particular, statistical support for the closeness of animals to fungi rather than to plants is lacking, and a preferred branching order of these and other eukaryotic lineages is still controversial even though molecular sequences from diverse eukaryotic taxa have been analyzed. We report a statistical analysis of 214 sequences of nuclear small-subunit ribosomal RNA (srRNA) gene undertaken to clarify these evolutionary relationships. We have considered the variability of substitution rates and the nonindependence of nucleotide substitution across sites in the srRNA gene in testing alternative hypotheses regarding the branching patterns of eukaryote phylogeny. We find that the rates of evolution among sites in the srRNA sequences vary substantially and are approximately gamma distributed with size and shape parameter equal to 0.76. Our results suggest that (1) the animals and true fungi are indeed closer to each other than to any other “crown” group in the eukaryote tree, (2) red algae are the closest relatives of animals, true fungi, and green plants, and (3) the heterokonts and alveolates probably evolved prior to the divergence of red algae and animal-fungus-green-plant lineages. Furthermore, our analyses indicate that the branching order of the eukaryotic lineages that diverged prior to the evolution of alveolates may be generally difficult to resolve with the srRNA sequence data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02198844
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  • 2
    Electronic Resource
    Electronic Resource
    Seasonal changes in levels of heavy metals in tissues of Mullus barbatus and Sparus aurata collected from Iskenderun Gulf (Turkey) (1996)
    Springer
    Water, air & soil pollution 90 (1996), S. 557-562 
    Details
    ISSN: 1573-2932
    Keywords: heavy metal ; accumulation ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Levels of cadmium, copper, zinc, lead and iron were determined seasonally in the liver, spleen, kidney, gill and muscle tissues of Mullus barbatus and Sparus aurata from the Iskenderun Gulf, East Mediterranean coast of Turkey. Wet digested tissues were analysed by atomic absorption spectroscopy. Metal levels were higher in liver, spleen and kidney compared with the gill and muscle tissues in both species; the levels of all metals in a given tissue were always higher in Mullus barbatus than in Sparus aurata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282669
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  • 3
    Electronic Resource
    Electronic Resource
    The influence of suspended silt on oxygen consumption by the redbreast tilapia, Tilapia rendalli (Boulenger,1896) (1996)
    Springer
    Hydrobiologia 325 (1996), S. 31-38 
    Details
    ISSN: 1573-5117
    Keywords: fish ; silt ; oxygen consumption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The routine oxygen consumption of redbreast tilapia, Tilapia rendalli exposed to silt from the Phalaborwa Barrage was investigated under controlled laboratory conditions. Exposure of adult fish to sublethal concentrations of silt did alter routine oxygen consumption by causing increased rates (53–77% from initial) of oxygen consumption. This indicates that sublethal levels of suspended silt concentrations results in an increase in oxygen consumption by the fish due to an increase in metabolic rate associated with increased activity and stress. From the laboratory experiments, it can therefore be predicted that sublethal releases from the Phalaborwa Barrage will stress the resident fish populations. However, the extent of the impact on the fish population will depend on factors such as frequency, duration and severity of exposure as well as the species in question. Long term monitoring of the fish population below the Phalaborwa Barrage is therefore essential.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023665
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  • 4
    Electronic Resource
    Electronic Resource
    Applying an index of biotic integrity based on fish assemblages in a West African river (1996)
    Springer
    Hydrobiologia 331 (1996), S. 71-78 
    Details
    ISSN: 1573-5117
    Keywords: fish ; biotic integrity ; river ; Guinea ; mormyridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A multivariate measure of river quality, the Index of Biotic Integrity (IBI), was adapted to a West African river, the Konkoure (Republic of Guinea). Fish assemblages were sampled using gill-nets during the dry season to provide data for the IBI. Ten metrics were subsequently defined. The capacity of the modified IBI to assess the impact of a bauxite treatment plant was tested. The IBI decreased as expected at the impacted station. Detailed examination of the data revealed that one family, the Mormyridae, was almost eliminated from the impacted station, suggesting that members of this familiy can be considered as intolerant species in future uses of the IBI in other African rivers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025409
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  • 5
    Electronic Resource
    Electronic Resource
    16. Dispersal of freshwater algae — a review (1996)
    Springer
    Hydrobiologia 336 (1996), S. 151-157 
    Details
    ISSN: 1573-5117
    Keywords: dispersal mechanisms ; water ; fish ; insects ; mammals ; birds ; air ; distances
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey is given on the various methods for dispersal of freshwater algae. Dispersal factors are either water or air, or organisms — from beetles, dragonflies and mammals to birds, the latter being the most important group. The question of dispersal distances is discussed, in relation to dispersal mechanisms and to the resistance of the algae to transport conditions. Man's recent importance in algal dispersal is emphasized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00010829
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of riparian land use on contributions of terrestrial invertebrates to streams (1996)
    Springer
    Hydrobiologia 337 (1996), S. 151-159 
    Details
    ISSN: 1573-5117
    Keywords: riparian zone ; grassland ; pasture ; forest ; fish ; diet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Since terrestrial invertebrates are often consumed by stream fishes, land-use practices that influence the input of terrestrial invertebrates to streams are predicted to have consequences for fish production. We studied the effect of riparian land-use regime on terrestrial invertebrate inputs by estimating the biomass, abundance and taxonomic richness of terrestrial invertebrate drift from 15 streams draining catchments with three different riparian land-use regimes and vegetation types: intensive grazing — exotic pasture grasses (4 streams), extensive grazing — native tussock grasses (6 streams), reserve — native forest (5 streams). Terrestrial invertebrate drift was sampled from replicated stream reaches enclosed by two 1 mm mesh drift nets that spanned the entire channel. The mean biomass of terrestrial invertebrates that entered tussock grassland (12 mg ash-free dry mass m−2 d−1) and forest streams (6 mg AFDM m−2 d−1) was not significantly different (p 〉 0.05). However, biomass estimated for tussock grassland and forest streams was significantly higher than biomass that entered pasture streams (1 mg AFDM m−2 d−1). Mean abundance and richness of drifting terrestrial invertebrates was not significantly different among land-use types. Winged insects contributed more biomass than wingless invertebrates to both pasture and tussock grassland streams. Winged and wingless invertebrates contributed equally to biomass entering forest streams. Land use was a useful variable explaining landscape-level patterns of terrestrial invertebrate input for New Zealand streams. Evidence from this study suggests that riparian land-use regime will have important influences on the availability of terrestrial invertebrates to stream fishes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028516
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  • 7
    Electronic Resource
    Electronic Resource
    Ratios of expected values (REX), a method for impact evaluation of thermal discharges on the fish community in the Pickering littoral waters of Lake Ontario (1996)
    Springer
    Hydrobiologia 328 (1996), S. 75-83 
    Details
    ISSN: 1573-5117
    Keywords: fish ; community ; power station ; evaluation ; discharge effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Discharge effects on the fish community are assessed for the Pickering power station and a creek in the neighbourhood, located in littoral waters of the northern shore of Lake Ontario. Since the ambience is cold in spring and fall, when the main spawning migration takes place, any positive thermal variation is selected with preference by the onshore migrants. Our goal is to identify these effects when significant or, alternatively, the community richness reduction, and even the biological damage to fish. Ratio to Expected Values (REX) involves a comparison of the diversity indexes from locations under impact versus ambient samples, in order to identify the effect magnitude. To clear the background records of local effects, they were checked for outliers, which have been replaced based on missing-value techniques. Applying the method, the slight thermal increase of the creek discharge was found to produce a higher richness compared to station discharges, hence offering more attraction for onshore migrants. Despite the high attraction of station thermal plumes, the general result is negative, due to a process described here as ambiguous attraction. It is preliminarily identified as a fish attraction, due to favourable thermal conditions at the plume edges, followed by repulsion and fish exhaustion, due to low oxygen level and absence of spawning conditions for the attracted fish, further in the plume. The paper also introduces a new statistical approach of effect evaluation for significance, based on pair comparison of records in the suspected area versus background records.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016901
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  • 8
    Electronic Resource
    Electronic Resource
    Structural phase diagrams of animal communities in assessment of freshwater ecosystem conditions (1996)
    Springer
    Hydrobiologia 322 (1996), S. 277-282 
    Details
    ISSN: 1573-5117
    Keywords: phase diagram ; dynamics of diversity ; Shannon's index ; steady state ; fish ; zooplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A phase diagram method, using indices of biological diversity, was applied to describe the dynamics of zooplankton and fish community structure in natural and experimental conditions. The phase diagram is constructed in the coordinates H′ and dH′ × dt−1, where H′ is the index of diversity of the community. The method reveals zones of steady state of a community and demonstrates community dynamics in undisturbed and disturbed conditions. Analysis of zooplankton dynamics in a fishless pond showed that in the absence of disturbances the community enters a zone of steady state (around dH′ × dt−1=0) and stays in it for a long time. Under the action of disturbances (e.g. oxygen depletion for fish, influence of heavy metals for zooplankton) the community leaves the steady state zone. The greater the disturbance, the more the system deviates from steady state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00031840
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  • 9
    Electronic Resource
    Electronic Resource
    Relationships between mesohabitats, ichthyological communities and IBI metrics adapted to a European river basin (The Meuse, Belgium) (1996)
    Springer
    Hydrobiologia 341 (1996), S. 133-144 
    Details
    ISSN: 1573-5117
    Keywords: fish ; Index of Biotic Integrity ; mesohabitats ; Belgium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the influence of mesohabitats on fish communities and on attributes of a multimetric index of fish integrity in the River Meuse basin. Three consecutive 150 m sectors in a Meuse tributary (Ourthe, Belgium), each divided in two or three sub-sections presenting various percentages of mesohabitats (riffles, runs and pools), were sampled by electrofishing. In each sub-section, relative and absolute biomasses of each fish species were estimated. The presence of limnophilic cyprinids was inversely correlated (r 2 = 0.70 and 0.56 for absolute and relative biomass, respectively) with the percentage of riffles. Salmonids preferred runs and their absolute biomass was highly dependent (r 2 = 0.71) on the proportion of this mesohabitat, whereas biomass of limnophilic cyprinids was highly correlated (r 2 = 0.75 and r 2 = 0.82 for absolute and relative biomass, respectively) with pools. A positive correlation (r 2 = 0.58) was established between relative biomass of predators and the percentage of this mesohabitat. An IBI was calculated for the three sectors on the basis of results from the entire Meuse catchment. Scores of most metrics showed low variation among sectors but values of two metrics (% of individuals as tolerant, % of individuals as ubiquitous spawners) were greatest in sector 2, where pools predominated. Further, the lowest IBI score (51/65, integrity class: fair to good) was recorded in sector 2 where pools dominated, while sector 1 (where runs dominated) obtained the highest score (63/65, integrity class: excellent). Sector 3 which has a balanced proportion of riffles and runs obtained an intermediate score (57/65). Considering the response of IBI to the natural variation of mesohabitat proportions, it appears that an accurate sampling requires the prospection of a variety of mesohabitats (with a majority of runs) for the evaluation of river quality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018117
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic variation and phenotypic plasticity in a trophically polymorphic population of pumpkinseed sunfish (Lepomis gibbosus) (1996)
    Springer
    Evolutionary ecology 10 (1996), S. 631-652 
    Details
    ISSN: 1573-8477
    Keywords: common environment ; reciprocal transplant ; genetic differentiation ; phenotypic plasticity ; trophic polymorphism ; evolution ; specialization ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Adaptive variation can exist at a variety of scales in biological systems, including among species, among local populations of a single species and among individuals within a single population. Trophic or resource polymorphisms in fishes are a good example of the lowest level of this hierarchy. In lakes without bluegill sunfish (Lepomis macrochirus), pumpkinseed sunfish (Lepomis gibbosus) can be trophically polymorphic, including a planktivorous limnetic form found in the pelagic habitat, in addition to the usual benthic form found in the littoral zone. In this paper we examine the degree to which morphological differences between the two forms are caused by genetic differences versus phenotypic plasticity. Adults from pelagic and littoral sites in Paradox Lake, NY, were bred separately and their progeny were raised in cages both in the open water and shallow water habitats of an artificial pond. The experimental design permitted two tests of genetic differences between the breeding stocks (in open and shallow water cages, respectively) and two tests of phenotypic plasticity (in the limnetic and benthic offspring, respectively). Limnetic progeny were more fusiform than benthic progeny raised in the same habitat. In addition, progeny of both stocks displayed limnetic-type characteristics when raised in the open water and benthic-type characteristics in the shallow water. Thus, genetic differences and phenotypic plasticity both contributed to the trophic polymorphism. Phenotypic plasticity and genetic differentiation accounted for 53 and 14%, respectively, of the variation in morphology. This study addresses the nature of subtle phenotypic differences among individuals from a single population that is embedded within a complex community, a condition that is likely to be the norm for most natural populations, as opposed to very large differences that have evolved in relatively few populations that reside in species-poor environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01237711
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  • 11
    Electronic Resource
    Electronic Resource
    A healthy Fraser River? How will we know when we achieve this state? (1996)
    Springer
    Journal of aquatic ecosystem stress and recovery 5 (1996), S. 107-115 
    Details
    ISSN: 1573-5141
    Keywords: biomonitoring ; community structure ; ecosystem health ; fish ; rivers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Assessing the condition of an ecosystem to ascertain its ‘health’ presupposes that we can diagnose pathological states in system measures. Frequently this means comparing current conditions to reference states, either historical or other sites, which also exhibit some natural range of variation. In the Fraser River, a 9th order river on the west coast of Canada, and one of the most productive salmon rivers in the world. we have studied assemblages of fish and benthos to assess ecosystem health. The biggest challenge to using species composition and abundance measures as indicators of system condition is the absence of appropriate reference conditions in many instances. There are few unperturbed rivers of large size in western North America, and indeed in much of the world, with which to compare the Fraser River or any other large river ecosystem. Multiple insults from point and non-point sources make it difficult to isolate factors from natural longitudinal changes in terms of their effects on river biota. Potential solutions include analysis of fragmentary historical data, making comparisons with other large rivers, and conducting extensive surveys within the basin to account for spatial gradients. An absolute diagnosis of ecosystem health of large rivers in natural science terms is unlikely, and otherwise will depend on relative changes through time assuming these can be isolated from natural variation and local effects. Definition of health for large, riverine ecosystems remains largely a case of expert opinion and weight of evidence rather than a testable hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00662799
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  • 12
    Electronic Resource
    Electronic Resource
    Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout (Oncorhynchus mykiss) semen (1996)
    Springer
    Fish physiology and biochemistry 15 (1996), S. 1-10 
    Details
    ISSN: 1573-5168
    Keywords: fish ; vitamin C ; spermatozoa ; seminal plasma ; proteinase inhibitors ; aspartate aminotransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During the reproductive season, rainbow trout spermatozoa are stored in the sperm ducts for several months. There is no sperm production at this time since spermatogenesis is completed before spawning. To leam more about characteristics of semen during such a long storage, we analyzed changes in protein concentrations, anti-proteinase activity in seminal plasma and sperm aspartate aminotransferase activity during an extended reproductive period during which fish were fed diets supplemented with various ascorbic acid concentrations. Seminal plasma protein concentration and anti-proteinase activity declined toward the end of the reproductive season. These phenomena may be related to oncoming proteolytic events leading to degradation of the sperm. Protein concentrations and anti-proteinase activities were strongly correlated within groups of different ascorbic acid supplementations and several sampling dates (r=0.6–0.9 in most cases, p〈0.05). Ascorbic acid deficiency resulted in a decrease in both parameter levels as compared to levels in groups with vitamin C supplement (p〈0.08). Deficiency also resulted in lower stimulation of aspartate aminotransferase by an exogenous pyridoxal 5′-phosphate in comparison to fish fed vitamin C-supplemented diets (p〈0.05). These results support earlier studies suggesting a protective role of ascorbic acid toward maintaining sperm quality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874832
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  • 13
    Electronic Resource
    Electronic Resource
    Immunochemical relationships of cytochrome P4503A-like proteins in teleost fish (1996)
    Springer
    Fish physiology and biochemistry 15 (1996), S. 323-332 
    Details
    ISSN: 1573-5168
    Keywords: cytochrome P450 ; CYP3A ; fish ; immunoblotting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Multiple P450 proteins have been purified from several teleost species, including rainbow trout (Oncorhynchus mykiss), scup (Stenotomus chrysops) and Atlantic cod (Gadus morhua). Identity, relationships and/or functions have been established in these fish species for the cytochrome P4501 As. Information about the structure, function, regulation and relationships of other piscine cytochrome P450 (CYP) proteins is sparse. In the present study we have focused on constitutively expressed CYP forms, P450con and LMC5 isolated from rainbow trout, P450A from scup, and P450b from Atlantic cod, and we consider evidence for the relationship of these proteins to mammalian members of the CYP3A subfamily. Reciprocal western blot analysis shows that P450con and LMC5, isolated from rainbow trout in two different laboratories, are closely related and ostensibly identical proteins. These trout proteins show specific reciprocal cross-reactivity with scup P450A, and polyclonal antibodies (PAb) to the trout and scup proteins both recognize cod P450b, indicating that rainbow trout P450con/LMC5, scup P450A and cod P450b are immunochemically-related proteins. In analyses of liver microsomes of trout, scup and cod, PAb to trout P450con/LMC5 and scup P450A recognize only bands that are identical in migration to the CYP proteins purified from these species, and which were used as immunogens. These CYP proteins purified from fish are each immunochemically-related to mammalian CYP3A proteins, showing recognition by PAb to human CYP3A4 and to rat CYP3A1. PAb to the mammalian CYP3As also recognize the same bands in liver microsomes from these fish species as seen by PAb to the fish proteins. These results strongly suggest that these fish proteins are members of theCYP3 gene family and probably theCYP3A subfamily. Although sequence analysis is required before their designation in the CYP3A subfamily can be confirmed and specified, we refer to these as CYP3A-like. Immunoblot analyses of hepatic microsomes from other fish species with PAb to scup P450A and trout P450con show that multiple CYP3A-like proteins are expressed in liver of several species, including killifish (Fundulus heteroclitus) and winter flounder (Pleuronectes americanus). Important questions still remain to be addressed concerning CYP3A structure, multiplicity, physiological function, regulation and metabolism of endogenous as well as exogenous substrates in fish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02112359
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  • 14
    Electronic Resource
    Electronic Resource
    Learning and sibling odor preference in juvenile arctic char,Salvelinus alpinus (L.) (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 773-786 
    Details
    ISSN: 1573-1561
    Keywords: Arctic char ; attraction ; fish ; fluviarium ; kinship ; odor ; salmonids ; Salvelinus alpinus ; siblings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The importance of learning for sibling odor preference in juvenile Arctic char was analyzed in the present study. Fish were reared in the following eight conditions: (1) communally with siblings for 15 months; (2) communally with siblings for 17 months; (3) in isolation since fertilization; (4) in isolation since fertilization and exposed to sibling scent during the whole rearing period; (5) in isolation since fertilization and exposed to sibling scent from time of free swimming; (6) in isolation since fertilization and exposed to sibling scent during the whole rearing period, except two months without scent until testing; (7) in isolation since fertilization and exposed to sibling scent from time of free swimming, except two months without scent until testing; and (8) communally with siblings followed by a two-month isolation until testing. Char were followed individually in a Y-maze (fluviarium test) with a video-computer-based image analysis system for 12 hr. Sibling-scented water was supplied to one lateral half of the test area and water from non-siblings on the opposite half. Isolated individuals without any preexposure to siblings showed no significant preference. Test fish reared with siblings and those that had been reared in isolation but exposed to sibling scent until testing preferred water conditioned by their own siblings. Isolated fish that had been exposed to sibling scent since fertilization, or since free swimming, followed by a two-month period with only pure water, showed no significant preference. Char isolated for two months after being communally reared preferred water scented by siblings. The results demonstrated that behavioral discrimination between siblings and nonsibling odors occurred after total isolation (isolated both from siblings and sibling odors) only in individuals that had been communally reared. This may suggest that social interactions are important for learning and long-term memory of sibling odors in Arctic char.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02033585
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  • 15
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    The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals (1996)
    Springer
    Cell biology and toxicology 12 (1996), S. 29-38 
    Details
    ISSN: 1573-6822
    Keywords: cadmium ; cell lines ; cytotoxicity ; fish ; mammalians ; primary cultures ; serum-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The differential cytotoxic effects of cadmium on fish and mammalian epithelial cells in established and primary culture were assessed by looking at the reduction of the colony-forming ability and reduction in the extent of growth. The influence of medium composition on the toxicity of cadmium was also studied using serum-free and serum-containing media. The experiments using immortalized cell lines showed that mammalian cells were more sensitive than fish cells to cadmium. Both cell types were grown at the same serum concentration. However, using the normal primary system, human epithelial tissue explants showed less sensitivity to cadmium than did similar cultures from rainbow trout. It is likely that cellular mechanisms of cadmium resistance in the different cell types are responsible for these effects. As expected, cadmium proved to be more toxic when tested in serum-free medium. With fish skin primary cultures, reduction of cell numbers was observed at concentrations as low as 5 μmol/L in serum-free medium compared to 100 μmol/L in serum-containing medium. This was found to be due to the reduction in the activity of free cadmium ions, caused by the presence of serum in the medium. It is concluded that serum-free formulations are probably preferable when testing pollutants in vitro. The results highlight the importance of establishing species-specific pollution tests and standardizing variables. In summary, the results show species and culture media differences in cadmium toxicity and suggest that caution is required when extrapolating results for pollutant effects from one in vitro system to another.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00143392
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  • 16
    Electronic Resource
    Electronic Resource
    Distribution of glutamine synthetase in the snapper (Pagrus auratus) and implications for the immune system (1996)
    Springer
    Fish physiology and biochemistry 15 (1996), S. 187-194 
    Details
    ISSN: 1573-5168
    Keywords: glutamine ; glutamine synthetase ; immunity ; stress ; teleost ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A sensitive radiotracer method was used to detect glutamine synthetase activity in fish. The distribution of glutamine synthetase activity using this method was shown to be widespread in organs and tissues of the marine finfish, snapper,Pagrus auratus. The optimum pH and temperature for glutamine synthetase activity and Km of glutamate from the brain of the snapper are also reported. Interestingly no glutamine synthetase activity was detected in lymphoid organs, indicating, that as in the mammal, the teleostean immune system is likely to rely on glutamine in circulation for energy and nucleotide synthesis. Furthermore circulating glutamine levels fell as fish became stressed or became ill. Implications of these observations on the function of the immune system of fish are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875569
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