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  • Articles  (91)
  • Life and Medical Sciences  (90)
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  • 1995-1999  (91)
  • 1955-1959
  • 1995  (91)
  • Physics  (91)
  • 1
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 16 (1995) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 2
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    Bioelectromagnetics 16 (1995), S. 1-1 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 3
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    Bioelectromagnetics 16 (1995), S. 2-8 
    ISSN: 0197-8462
    Keywords: ELF fields ; biological effects ; Poisson processes ; threshold detection ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: If a weak, exogenous, extremely low-frequency (ELF) electric or magnetic field is to produce biological sequelae, then there must exist averaging sufficient to lift some primary effect of that field above the endogenous stochastic variations of the biological system. One way in which a field could accomplish this is by changing the intensity of some stochastic operation that controls an important and not trivially reversible biological transformation. In this paper, this operation is modeled as a doubly stochastic Poisson process. It is then shown, first, that (in theory) even a minuscule exogenous influence might appreciably shift the incidence of a sufficiently rare transformation and, second, that this shift might be observable if a trial were allowed to run long enough over a sufficiently large population of exposed entities. © 1995 Wiley-Liss, Inc.
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  • 4
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    Bioelectromagnetics 16 (1995), S. 11-11 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 5
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    Bioelectromagnetics 16 (1995), S. 12-16 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 6
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    Bioelectromagnetics 16 (1995), S. 17-19 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 7
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    Bioelectromagnetics 16 (1995), S. 97-105 
    ISSN: 0197-8462
    Keywords: atrioventricular block ; heat coagulation ; reversible and irreversible block ; catheter antenna ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The use of microwave energy for ablation of the atrioventricular (AV) junction was examined in open-chest dogs. Using a specially designed microwave catheter and a 2450 MHz generator, microwave energy was delivered to the AV junction according to one of two protocols. In protocol 1, increasing amounts of energy were delivered until irreversible AV block occurred. In protocol 2, only two applications of energy were used, one at low energy and the other at an energy found to be high enough to cause irreversible AV block. Each dog received between one and six applications of microwave energy. The amount of energy delivered per application ranged from 25.6 to 311.4 J. No AV block was seen at 59.4 ± 28.3 J. Reversible AV block was seen with an energy of 120.6 ± 58 J. Irreversible AV block was seen at 188.1 ± 75.9 J. Irreversible AV block could be achieved in each animal. There was no difference in the energy required to cause irreversible AV block between the two protocols. The tissue temperature measured near the tip of the microwave catheter was correlated with both the amount of energy delivered and the extent of AV block caused. Histologic examination demonstrated coagulation necrosis of the conduction system. Microwave energy is a feasible alternative energy source for myocardial ablation. Since tissue damage is due exclusively to heating and the resulting rise in temperature can be measured, microwave energy may have advantages over currently existing energy sources in terms of both titrating delivered energy and monitoring the extent of tissue destruction. © 1995 Wiley-Liss, Inc.
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  • 8
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    Bioelectromagnetics 16 (1995), S. 160-171 
    ISSN: 0197-8462
    Keywords: RF radiation ; E-field ; SAR ; cell model ; membrane free water ; bound water ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The spatial distributions of induced 27 or 2450 MHz radiofrequency (RF) electric fields (E-fields) and specific absorption rates (SARs) in a three-component spherical cell model (cytoplasm, membrane, extracellular space) were determined by Mie scattering theory. The results were compared to results for the same cell model but with 0.5 nm thick of bound water on the inner (cytoplasmic) and outer (extracellular) membrane surfaces (i.e., five-component cell model). The results provide insight regarding direct frequency-dependent RF radiation effects at the cellular level. Induced E-fields and SARs were calculated for two bound-water characteristic frequencies (400 or 1000 MHz) and ionic conductivities (1-1000 mS/m). In order to estimate the dependence of the results on bound water within the membrane per se, the model was revised to include bound water within the inner and outer membrane surfaces. The results were as follows: (1) On the x-axis, the y- and z-components of the induced E-field were of insignificant magnitude compared to the x-component for an incident E-field parallel to the x-axis; (2) the ratio of transmembrane E-fields induced by 2450 MHz vs. 27 MHz RF [i.e., Ex (2450 MHz)/Ex (27 MHz)] was 0.1; (3) for the three-component cell model, the corresponding SAR ratios [SAR (2450 MHz)/SAR (27 MHz)] in the cytoplasm and extracellular space were 1.66 and 5.0, respectively; (4) the SAR ratios [SAR (2450 MHz)/SAR (27 MHz)] for the cytoplasm and extracellular space for the five-component cell model were 1.66 and 5.0, respectively; (5) the ratio of the E-fields induced in the cytoplasmic and extracellular layers of bound water in the five-component cell model [E (2450 MHz)/E (27 MHz)] were 0.62 and 0.63, respectively; (6) the SAR ratios [SAR (2450 MHz)/SAR (27 MHz)] for the cytoplasmic and extracellular bound-water layers were 66 and 65.3, respectively; and (7) variation of bound-water characteristic frequency, ionic conductivity, or bound-water incorporation inside the membrane surfaces, per se, did not significantly affect the E-field or SAR ratios. These results indicate that frequency-dependent nonuniformities may occur in the distribution of induced RF E-fields and SARs at the cellular level. © 1995 Wiley-Liss, Inc.
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  • 9
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    Bioelectromagnetics 16 (1995), S. 402-404 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Dr. Peter Valberg has written an excellent and clear paper describing our current understanding of the many facets of characterizing exposure to extremely-low-frequency (ELF) electric and magnetic fields. Dr. Valberg has directed his paper to the characterization of exposures used in laboratory experimentation. In this context, it seems to me that Valberg's program of exposure characterization can be accomplished with currently available instrumentation and with relatively modest effort. Thus, I have no fundamental problems with his recommendations and, really, have only a few minor comments to make. However, if one argues that Valberg's recommendations should be extended to the characterization of exposure in epidemio-logical research, I have serious reservations, which I will mention briefly at the end of this commentary. © 1995 Wiley-Liss, Inc.
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  • 10
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    Bioelectromagnetics 16 (1995), S. 124-131 
    ISSN: 0197-8462
    Keywords: millimeter microwaves ; ionic channels ; lipid-soluble ions ; bilayer membranes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of millimeter microwaves in the frequency range of 54-76 GHz on capacitance and conductance of lipid bilayer membranes (BLM) were studied. Some of the membranes were modified by gramicidin A and amphotericin B or by tetraphenylboron anions (TPhB-). The millimeter microwaves were pulse-modulated (PW) at repetition rates ranging from 1 to 100 pps, PW at 1000 pps, or unmodulated continuous waves (CW). The maximum output power at the waveguide outlet was 20 mW. It was found that CW irradiation decreased the unmodified BLM capacitance by 1.2% ± 0.5%. At the same time, membrane current induced by TPhB transport increased by 5% ± 1%. The changes in conductance of ionic channels formed by gramicidin A and amphotericin B were small (0.6% ± 0.4%). No “resonance-like” effects of mm-wave irradiation on membrane capacitance, ionic channel currents, or TPhB transport were detected. All changes in membrane capacitance and currents were independent of the modulation employed and were equivalent to heating by approximately 1.1 °C. © 1995 Wiley-Liss, Inc.
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  • 11
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    Bioelectromagnetics 16 (1995), S. 106-112 
    ISSN: 0197-8462
    Keywords: extremely low frequency (ELF) ; magnetic field ; ion cyclotron resonance ; planarian regeneration ; orientation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Extremely-low-frequency (ELF), low-intensity magnetic fields have been shown to influence cell signaling processes in a variety of systems, both in vivo and in vitro. Similar effects have been demonstrated for nervous system development and neurite outgrowth. We report that regeneration in planaria, which incorporates many of these processes, is also affected by ELF magnetic fields. The rate of cephalic regeneration, reflected by the mean regeneration time (MRT), for planaria populations regenerating under continuous exposure to combined DC (78.4 μT) and AC (60.0 Hz at 10.0 μT peak) magnetic fields applied in parallel was found to be significantly delayed (P ≪ 0.001) by 48 ± 1 h relative to two different types of control populations (MRT ˜ 140 ± 12 h). One control population was exposed to only the AC component of this field combination, while the other experienced only the ambient geomagnetic field. All measurements were conducted in a low-gradient, low-noise magnetics laboratory under well-maintained temperature conditions. This delay in regeneration was shown to be dependent on the planaria having a fixed orientation with respect to the magnetic field vectors. Results also indicate that this orientation-dependent transduction process does not result from Faraday induction but is consistent with a Ca2+ cyclotron resonance mechanism. Data interpretation also permits the tentative conclusion that the effect results from an inhibition of events at an early stage in the regeneration process before the onset of proliferation and differentiation. © 1995 Wiley-Liss, Inc.
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  • 12
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    Bioelectromagnetics 16 (1995), S. 144-144 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 13
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    Bioelectromagnetics 16 (1995), S. 145-145 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 14
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    Bioelectromagnetics 16 (1995), S. 143-143 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 15
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    Bioelectromagnetics 16 (1995), S. 132-142 
    ISSN: 0197-8462
    Keywords: ELF ; epidemiology ; exposure assessment ; misclassification ; statistical power ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A computer program simulating case-control studies is described. It is used to estimate the minimum sample size required and to assess how this is affected by imprecise exposure assessment. In particular, the consequences of neglecting measurements of nonresidential exposure in case-control studies of residentially exposed adults are investigated. According to this model, while the consequent loss of power is not as large as was predicted by algebraic methods, it would be unwise to neglect it when planning a study. © 1995 Wiley-Liss, Inc.
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  • 16
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    Bioelectromagnetics 16 (1995) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 17
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    Bioelectromagnetics 16 (1995), S. 152-159 
    ISSN: 0197-8462
    Keywords: EMFs ; biomechanics ; bone ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Whole-body fields were tested for their efficacy in preventing the osteopenia caused by tail suspension in mice. The fields had fundamental frequencies corresponding to the upper range of predicted endogenous impact-generated frequencies (0.25-2.0 kHz) in the long bones. Three distinct whole-body EMFs were applied for 2 weeks on growing mice. Structural, geometric, and material properties of the femora, tibiae, and humeri of suspended mice were altered compared to controls. Comparison of suspended mice and mice subjected to caloric restriction indicates that the changes in caloric intake do not explain either the suspension or the field-induced effects. In agreement with past studies, rather, unloading appears to cause the suspension effects and to be addressed by the EMFs. The EMF effects on bone properties were apparently frequency dependent, with the lower two fundamental frequencies (260 and 910 Hz) altering, albeit slightly, the suspension-induced bone effects. The fields are not apparently optimized for frequency, etc., with respect to therapeutic potential; however, suspension provides a model system for further study of the in vivo effects of EMFs. © 1995 Wiley-Liss, Inc.
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  • 18
    ISSN: 0197-8462
    Keywords: magnetic field ; ATPase ; resting membrane potential ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In this study, we aimed to clarify the effects of chronically applied static magnetic field (200 Gauss) on specific ATPase activities and bioelectrical and biomechanical responses in the isolated rat diaphragm muscle. The mean activities of Na+-K+ ATPase and Ca2+ ATPase determined from the diaphragm homogenates were significantly higher in the magnetic field exposed group (n = 20), but that of Mg2+ ATPase was nonsignificantly lower compared to the control group (n = 13). Resting membrane potential, amplitude of muscle action potential, and overshoot values (mean ± SE) in the control group were found to be -76.5 ± 0.6, 100 ± 0.8, and 23.5 ± 0.6 mV, respectively; these values were determined to be -72.8 ± 0.4, 90.3 ± 0.5, and 17.2 ± 0.4 mV in the magnetic field-exposed group, respectively. The latency was determined to increase in the experimental group, and all the above-mentioned bioelectrical differences between the groups were significant statistically. Force of muscle twitch was found to decrease significantly in the magnetic field-exposed group, and this finding was attributed to the augmenting effect of magnetic field on Ca2+ ATPase activity. These results suggest that magnetic field exposure changes specific ATPase activities and, thence, bioelectrical and biomechanical properties in the rat diaphragm muscle. © 1995 Wiley-Liss, Inc.
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  • 19
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    Bioelectromagnetics 16 (1995), S. 172-177 
    ISSN: 0197-8462
    Keywords: effects function ; EMDEX ; simulation ; dose ; dose response ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A method for evaluating a variety of alternative biologically plausible effects functions through the use of simulation studies conducted on personal-monitor exposure data is described. Using magnetic field time series collected with EMDEX instruments, we demonstrate how the method can be used to explore (1) how the outputs from various effects functions simulations compare to the results obtained by assuming that effects are proportional to time average field strength; (2) how the results of epidemiological studies might be used to assess the relative likelihood that each of the alternative effects functions describes biological reality; and (3) how the results might be used to assess possible health risks. Although the available data are sufficient to demonstrate the general method, they are not yet sufficient to support actual discrimination among possible alternatives. The arguments on the use of the method are for illustrative purposes only. © 1995 Wiley-Liss, Inc.
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  • 20
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    Bioelectromagnetics 16 (1995), S. 188-196 
    ISSN: 0197-8462
    Keywords: electromagnetic fields ; microwaves ; ultraviolet ; bacteria ; biological effect ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Bacterial cells of the strain Escherichia coli K12 were exposed to millimeter electromagnetic waves (mm waves) with and without additional exposure to ultraviolet light λ = 254 nm (UVC). The mm waves were produced by a medical microwave generator emitting a 4-GHz-wide band around a 61 GHz center frequency and delivering an irradiation of 1 mW/cm2 and a standard absorption rate (SAR) of 84 W/kg to the bacteria. Exposure to the mm waves alone for up to 30 minutes did not change the survival rate of bacteria. Exposure to mm waves followed by UVC irradiation also did not alter the number of surviving E. coli cells in comparison to UVC-treated controls. When mm waves were applied after the UVC exposure, a dose-dependent increase of up to 30% in the survival of E. coli was observed compared to UVC + sham-irradiated bacteria. Because sham controls and experimental samples were maintained under the same thermal conditions, the effect is not likely to be due to heating, although the possibility of nonuniform distribution of microwave heating in different layers of irradiated bacterial suspension cannot be ruled out. The mechanism for this effect appears to involve certain DNA repair systems that act as cellular targets for mm waves. © 1995 Wiley-Liss, Inc.
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  • 21
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    Bioelectromagnetics 16 (1995), S. 71-92 
    ISSN: 0197-8462
    Keywords: Papio cynocephalus anubis ; biological effects ; central nervous system ; nonhuman primates ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Our previous research has demonstrated that 30 or 60 kV/m electric fields (EF) reliably produce temporary increases in the performance of three categories of baboon social behavior: Passive Affinity, Tension, and Stereotypy. The experimental design included 6 week preexposure, exposure, and postexposure periods with experimental and control groups, each with eight subjects. Here, we report two experiments that evaluated the effects of combined EF and magnetic fields (MF) on baboon social behavior. One experiment demonstrated that exposure to 6 kV/m EF and 50 μT (0.5 G) MF produced Period × Group interactions for Stereotypy and Attack, but the previously observed increases in Passive Affinity, Tension, and Stereotypy did not occur. A second experiment demonstrated that exposure to 30 kV/m EF and 100 μT 1.0 G MF did not produce the same magnitude of increases in Passive Affinity, Tension, and Stereotypy observed previously with 30 kV/m EF alone. The exposed group exhibited decreased performance rates for several behavior categories during exposure with further declines during postexposure. The control group showed fewer downward trends across periods. © 1995 Wiley-Liss, Inc.
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  • 22
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    Bioelectromagnetics 16 (1995), S. 227-230 
    ISSN: 0197-8462
    Keywords: geomagnetic metric ; ion cyclotron resonance ; epidemiological design ; power line orientation ; childhood leukemia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The metric of prime interest in power line epidemiological studies has been AC magnetic intensity. To consider also possible geomagnetic involvement, the orientation of a long straight power line is examined relative to a uniform geomagnetic field (GMF) with dip angle α. An expression is derived for the component of the total GMF that is parallel, at an elevation β, to the circuital magnetic field that surrounds the line. This component is a function of the angles α and β, the total geomagnetic intensity BT, and the angle θ between the axis of the power line and magnetic north. Plotting these geomagnetic parameters for known leukemia residences allows one to test for possible ion cyclotron resonance or other GMF interactions. This approach, in principle, is an easy addition to existing or planned studies, because residential access is not required to obtain local values for α, β, θ, and BT. We recommend including these parameters in the design of epidemiological studies examining power line fields and childhood leukemia. © 1995 Wiley-Liss, Inc.
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  • 23
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    Bioelectromagnetics 16 (1995), S. 216-226 
    ISSN: 0197-8462
    Keywords: job classification ; occupational exposure ; personal-exposure monitoring ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The Electric and Magnetic Field Measurement Project for Utilities - the Electric Power Research Institute (EPRI) Electric and Magnetic Field Digital Exposure (EMDEX) Project (the EPRI EMDEX Project) - was a multifaceted project that entailed technology transfer, measurement protocol design, data management, and exposure assessment analyses. This paper addresses one specific objective of the project: the collection, analysis, and documentation of power-frequency magnetic field exposures for a diverse population of utility workers. Field exposure data measured by an EMDEX system were collected by volunteer utility employees at 59 sites in four countries between September, 1988, and September, 1989. Specially designed sampling procedures and data collection protocols were used to ensure uniform implementation across sites. Volunteers within 13 job classifications recorded which of eight work or three nonwork environments they occupied while wearing an EMDEX meter. Approximately 50,000 hours of magnetic field exposure records taken at 10 s intervals were obtained, about 70% of which were from work environments. Exposures and time spent in environments were analyzed by primary work environment, by occupied environment, and by job classification. Generally, for utility-specific job classifications related to the generation, transmission, and distribution of electricity, the field and exposure measurements in terms of workday mean field were higher than in more general occupations. The job classifications with the highest (median workday mean) exposure were substation operators (0.7 μT) and electricians (0.5μT). Total variance also tended to be largest for utility-specific job classifications. For these workers, the contributions of between-worker and within-worker variances to total variance were about the same. Measurements in utility-specific environments were higher than in more general environments. Estimates of time-integrated exposure indicated that utility-specific job classifications received about one-half or more of their total exposure on the job. The nonwork field and exposure distributions for workers in all job categories were comparable with median nonworkday means of about 0.09 μT. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 250-254 
    ISSN: 0197-8462
    Keywords: electromagnetic field ; frog auricle ; caffeine ; propranolol ; atropine ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The combined effects of microwave radiation and some drugs were studied in an isolated frog auricle preparation. The experiments established that exposure to pulse-modulated 915 MHz microwaves for up to 40 min had no effect on either the rate or the amplitude of spontaneous auricle twitches, unless the average absorbed power was high enough to produce preparation heating. Treatment of the preparation with saline containing (0.6-3.0) 10-5 M of propranolol or (0.5-1.5) 10-7 M of atropine altered neither its pacemaker nor its contractile functions; these drugs also had no effect when they were combined with nonthermal microwave irradiation. Caffeine (1 mM) strongly increased the average heart power, which was calculated as the product of twitch rate and amplitude. The caffeine effect appeared to be significantly augmented (by about 15%, P〈0.02) under exposure to burst-type pulsed microwaves (pulse width, 1.5 msec; pause, 2.5 msec; 8 pulses/burst, 16 bursts/s; average SAR, 8-10 W/kg). By itself, this modulation was not effective; the heating of the preparation and saline during exposure was approximately 0.1°C, which could not account for the detected changes. The experimental results demonstrate that caffeine treatment increases the microwave sensitivity of the frog auricle preparation and reveals primarily subthreshold, nonthermal microwave effect. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 268-269 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 255-262 
    ISSN: 0197-8462
    Keywords: electrical stimulation ; electrotonic ; hyperpolarization ; depolarization ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Pulsed electric fields directly influence the electrophysiology of tissue cells by transiently perturbing their transmembrane potential. To determine the magnitude and time course of this interaction, electrotonic cable theory was used to calculate the membrane potential perturbations induced in tissue cells by a spatially uniform, pulsed electric field. Analytic solutions were obtained that predict shifts in membrane potential along the length of cells as a function of time in response to an electrical pulse. For elongated tissue cells, or groups of tissue cells that are coupled electrotonically by gap junctions, significant hyperpolarizations and depolarizations can result from millisecond applications of electric fields with strengths on the order of 10-100 mV/cm. The results illustrate the importance of considering cellular cable parameters in assessing the effects of transient electric fields on biological systems, as well as in predicting the efficacy of pulsed electric fields in medical treatments. © 1995 Wiley-Liss, Inc.
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 335-336 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 339-340 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 365-376 
    ISSN: 0197-8462
    Keywords: nationwide EMF exposure ; transmission grid ; geographical information system ; cancer risk ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In a specific case, the magnetic field generated in a building by a nearby power line is usually easy to calculate, although the accuracy of these calculations is sensitive to the quality of source information. To be able to study public health dimensions of magnetic field exposure (e.g., risk of cancer), it is necessary to evaluate the size and exposure of the population at risk. Relatively little quantitative information on public exposure to power-frequency magnetic fields of high-voltage power lines is available. This report describes residential exposure to magnetic fields from 110 kV, 220 kV, and 400 kV power lines in Finland at the national level, including 90% of the total line length in 1989. A geographical information system (GIS) was used to identify the buildings located near the power lines. After determining the distances between the lines and the buildings, historical data on load currents of these lines were used to calculate the magnetic fields. The residential magnetic field histories were then linked to the residents by means of a computerized central population register. The data obtained on personal exposure have also been utilized in a nationwide epidemiological study on magnetic field exposure of power lines and risk of cancer. The methods of exposure assessment and results of the number of buildings near 110 kV, 220 kV, and 400 kV power lines, their average annual magnetic fields, and personal exposure to magnetic fields from these lines are described. We found that 15,600 residents lived in an average residential magnetic field ≥0.1 μT caused by power lines in 1989. The number of these residents increased fivefold during 1970-1989. We estimated that 0.3% of the population was exposed in their residences to an annual average magnetic flux density from 110 kV, 220 kV, and 400 kV power lines higher than 0.1 μT, the level that the background magnetic flux density in general does not exceed in Finnish homes. Thus, the problem of magnetic field exposure generated by high-voltage lines concerns only a relatively small fraction of the total population in Finland. However, the size and exposure of the population at risk remain somewhat arbitrary in practical multisource situations, as the biological interaction mechanism, the concept of harmful dose, and, in particular, the significance of the duration of exposure are unknown. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 381-386 
    ISSN: 0197-8462
    Keywords: ELF magnetic fields ; cAMP ; cell communication ; multicellular spheroids ; threedimensional cell contact ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: To investigate the influence of physiological parameters such as cell density and three-dimensional cell contact on the biological action of a 2 mT/50 Hz magnetic field, mouse fibroblasts were exposed as monolayers and as multicellular spheroids. Changes in cyclic AMP content of cells and alterations in gap junction-mediated intercellular communication were measured immediately after 5 min of exposure to the field. In monolayers of intermediate cell density (1 × 105 cells/cm2), the field treatment caused an increase in cAMP to 121% of the control level, whereas, at 3 × 105 cells/cm2 (near confluence), a decrease to 88% of the unexposed cells was observed. Furthermore, field exposure stimulated gap-junction communication to 160% of the control level as determined by Lucifer yellow dye exchange. In spheroids, alterations in the radial profile of cellular cAMP were observed that were due both to field-induced local cAMP changes and to increased gap-junction permeability for this second messenger, the latter causing radial cAMP gradients to be flattened. The results indicate a strong dependence of field action on physiological parameters of the system exposed. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 387-395 
    ISSN: 0197-8462
    Keywords: ELF ; in vitro ; dose response ; orthogonal AC/DC magnetic fields ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: We have shown that 50 Hz sinusoidal magnetic fields within the 5-10 micro Tesla (μT) rms range cause an intensity-dependent reduction in nerve growth factor (NGF) stimulation of neurite outgrowth (NO) in PC-12 cells. Here we report on the frequency dependence of this response over the 15-70 Hz range at 5 Hz intervals. Primed PC-12 cells were plated in collagen-coated, 60 mm plastic petri dishes with or without 5 ng/ml NGF and were exposed to sinusoidal magnetic fields for 22 h in a CO2 incubator at 37 °C. One 1,000-turn coil, 20 cm in diameter, generated vertically oriented magnetic fields. The dishes were stacked on the center axis of the coil to provide a range of intensities between 3.5 and 9.0 μT rms. The flux density of the ambient DC magnetic field was 37 μT vertical and 19 μT horizontal. The assay consisted of counting over 100 cells in the central portion (radius ≤0.3 cm) of each dish and scoring cells positive for NO. Sham exposure of cells treated identically with NGF demonstrated no difference in the percentage of cells with NO between exposed and magnetically shielded locations within the incubator. Analysis of variance demonstrated flux density-dependent reductions in NGF-stimulated NO over the 35-70 Hz frequency range, whereas frequencies between 15 Hz and 30 Hz produced no obvious reduction. The results also demonstrated a relative maximal sensitivity of cells at 40 Hz with a possible additional sensitivity region at or above 70 Hz. These findings suggest a biological influence of perpendicular AC/DC magnetic fields different from those identified by the ion parametric resonance model, which uses strictly parallel AC/DC fields. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 342-342 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 377-380 
    ISSN: 0197-8462
    Keywords: melatonin ; pineal gland ; aMT6s ; ELF ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effect of a 50 Hz, vertical magnetic field on the excretion of urinary 6-sulphatoxymelatonin (aMT6s) of male and female Wistar rats was studied in a self-controlled experiment. Twenty rats were kept in metabolic cages under 9:15 h light:dark conditions. The urine of the animals was collected twice per day for 5 consecutive days. The concentration of aMT6s in the rat urine was measured by 125I radioimmunoassay. The rats were exposed to 5 and 500 μT flux density for 24 h. The excretion of urinary aMT6s did not show significant changes during or after magnetic field exposure. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 343-355 
    ISSN: 0197-8462
    Keywords: ELF magnetic field ; growth curve ; histology ; hematology ; serum chemistry ; neurotransmitters ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: To provide possible laboratory support to health risk evaluation associated with long-term, low-intensity magnetic field exposure, 256 male albino rats and an equal number of control animals (initial age 12 weeks) were exposed 22 h/day to a 50 Hz magnetic flux density of 5 μmT for 32 weeks (a total of about 5000 h). Hematology was studied from blood samples before exposure to the field and at 12 week intervals. Morphology and histology of liver, heart, mesenteric lymph nodes, and testes as well as brain neurotransmitters were assessed at the end of the exposure period. In two identical sets of experiments, no significant differences in the investigated variables were found between exposed and sham-exposed animals. It is concluded that continuous exposure to a 50 Hz magnetic field of 5 μT from week 12 to week 44, which makes up ˜70% of the life span of the rat before sacrifice, does not cause changes in growth rate, in the morphology and histology of liver, heart, mesenteric lymph nodes, testes, and bone marrow, in hematology and hematochemistry, or in the neurotransmitters dopamine and serotonin. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 396-401 
    ISSN: 0197-8462
    Keywords: electric fields ; magnetic fields ; power lines ; ELF electromagnetic fields ; EMF metrics ; descriptive parameters ; exposure indices ; replication ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Anyone who has attempted to organize and synthesize the results of research on biological effects of electric and magnetic fields (EMF) has experienced frustration when trying to evaluate the comparability of EMF exposures among separate studies. Reporting of exposure characteristics is often incomplete, and some investigators focus on particular nuances of exposure, which in other laboratories go unrecorded because they are not regarded as important. The obstacles encountered when comparing studies, when designing replication studies, and when evaluating research proposals could be reduced were a more standardized approach taken in describing “EMF exposure.” To this end, a numerical listing of 18 separate parameters important to EMF exposure characterization is proposed. Although the goal of this list is primarily to expedite the description of EMF exposure, references are provided to examples of EMF exposures and to detailed discussions of EMF exposure systems. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 35-47 
    ISSN: 0197-8462
    Keywords: baboon ; Papio cynocephalus ; multiple schedule ; FR30 ; DRL20 ; work stoppage ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In two separate experiments, we examined the effects of a 60 Hz electric field (EF) on performance of an operant schedule consisting of two signaled components: fixed-ratio (FR30) and differential reinforcement of low-rate (DRL20). In each experiment, 12 naive baboons (Papio cynocephalus) were assigned randomly to either an EF-exposed experimental group or a sham-exposed control group. A homogeneous vertical EF of 30 kV/m was used in one experiment; 60 kV/m was used in the other. The experimental design for both experiments included 6 week preexposure, exposure, and postexposure periods. The planned analyses indicated no evidence of statistically significant (P 〈.05) effects of EF exposure. However, exploratory analyses comparing performance during the last week of preexposure and the first week of exposure revealed statistically significant acute effects (work stoppage): The mean response rates of the EF-exposed groups were greatly reduced on day 1 of exposure but were normal by the end of day 2 of EF exposure. We hypothesize that introduction of a highly unusual stimulus, the EF, temporarily interfered with normal operant behavior to produce a primary work stoppage. Supplementary cross-over experiments added at the end of each main experiment indicated that work stoppage occurred again when formerly EF-exposed subjects served as sham-exposed controls, while other subjects received their first EF exposure. Presumably, reoccurrence of other stimuli correlated with initial exposure to the EF became sufficient to subsequently cause secondary work stoppage in the absence of direct EF exposure. The primary and secondary work-stoppage effects were reproducible. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 48-60 
    ISSN: 0197-8462
    Keywords: aversion ; baboon (Papio cynocephalus) ; operant responding ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Using a set of six baboons (Papio cynocephalus), we conducted a series of seven experiments designed to evaluate the potentially aversive character of a 60 Hz electric field (EF). Initially, the subjects were trained, using food rewards as the reinforcer, to respond only when a cue light was illuminated. Next, an EF was presented along with the cue light; responses produced delivery of a food pellet and turned off both the cue light and the EF. Then, stimulus and reward conditions were varied. We determined that (1) presence of a strong EF does not affect operant responding for food rewards, (2) subjects will not respond at normal rates when the only reinforcer is termination of a strong EF, (3) presence of a strong EF can serve as a discriminative stimulus, (4) presence of a strong EF does not affect extinction of an appetite-motivated task, and (5) presentation of an EF can become a secondary reinforcer. The pattern of results was consistent across all experiments, suggesting that an EF of as much 65 kV/m is not aversive to nonhuman primates. Separately, we demonstrated that the average EF detection threshold for baboons is 12 kV/m. Thus, EF exposure at intensities well above the detection threshold and at species-scaled EF strengths greater than those found environmentally does not appear to be aversive. © 1995 Wiley-Liss, Inc.
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    ISSN: 0197-8462
    Keywords: Papio cynocephalus ; short-term memory ; operant behavior ; learning ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: We examined the effects of combined 60 Hz electric and magnetic field (EMF) exposure on performance of delayed match-to-sample (MTS) procedure involving the flash rate of a light as the stimulus. Six baboons (Papio cynocephalus) fully acquired the task; four others functioned accurately only when cued. All ten subjects were assigned to EMF-exposed or sham-exposed groups of five and were used to test for a work-stoppage effect that was previously observed with initial exposure to electric fields (EF) of 30 or 60 kV/m. Here, we report the results of two experiments, each consisting of 6 week preexposure, exposure, and postexposure periods. We found no evidence of work stoppage with fields of 6 kV/m and 50 μT (0.5 G) or with 30 kV/m and 100 μT (1.0 G). In neither experiment was there evidence of an adverse effect of 60 Hz EMF exposure on MTS performance. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 178-187 
    ISSN: 0197-8462
    Keywords: fibroblasts ; image cytometry ; cell migration ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Automated image cytometry techniques were used to measure motility and morphology in 3T3 fibro-blasts exposed to extremely-low-frequency (ELF) magnetic fields. Cell motility and morphology were measured as a function of time before, during, and after 3-4 hour exposures to vertically oriented, 100 μTRMS sinusoidal magnetic fields at various frequencies in the 10-63 Hz range. Sham exposures were also carried out. No static DC fields were applied, but the geomagnetic field was almost vertical and, therefore, had a large component (28.3 μT) parallel to the applied AC field. The morphology and motile behavior of the cells were characterized by mathematically defined descriptors, which were calculated and averaged for the exposure period as well as for control periods that preceded and followed the exposure period. Each experiment involved the tracking of 100 cells that were subjected to one of the test frequencies (unless a sham exposure was being conducted). Statistical analysis of the results showed that even small changes of 10-20% could be significant at the P 〈.05 level. Changes on this order were measured in a significant proportion of the experiments. However, because such results were seen for both the sham-exposed and the ELF-exposed cells, and because the range of values that was obtained for the sham exposures was the same as that obtained for the ELF exposures, we concluded that there was no evidence to show that any of the measured changes were attributable to the applied ELF magnetic field. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995) 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 197-206 
    ISSN: 0197-8462
    Keywords: static magnetic fields ; spatial field variation ; computer model ; gradient ; action potential blockade ; cultured sensory neurons ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: To characterize the properties of static magnetic fields on firing of action potentials (AP) by sensory neurons in cell culture, we developed a mathematical formalism based on the expression for the magnetic field of a single circular current loop. The calculated fields fit closely the field measurements taken with a Hall effect gaussmeter. The biological effect induced by different arrays of permanent magnets depended principally on the spatial variation of the fields, quantified by the value of the gradient of the field magnitude. Magnetic arrays of different sizes (macroarray: four center-charged neodymium magnets of ˜14 mm diameter; microarray: four micromagnets of the same material but of ˜0.4 mm diameter) allowed comparison of fields with similar gradients but different intensities at the cell position. These two arrays had a common gradient value of ˜1 mT/mm and blocked 〉70% of AP. Alternatively, cells placed in a field strength of ˜0.2 mT and a gradient of ˜0.02 mT/mm produced by the macroarray resulted in no significant reduction of firing; a microarray field of the same strength but with a higher gradient of ˜1.5 mT/mm caused ˜80% AP blockade. The experimental threshold gradient and the calculated threshold field intensity for blockade of action potentials by these arrays were estimated to be ˜0.02 mT/mm and -0.02 mT, respectively. In conclusion, these findings suggest that spatial variation of the magnetic field is the principal cause of AP blockade in dorsal root ganglia in vitro. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 290-294 
    ISSN: 0197-8462
    Keywords: stress-induced analgesia ; pigeons ; behavior ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The comparison of two measurements of the pigeon threshold for electrical stimuli, performed 2 h apart, reveals stress-induced analgesia as a result of stressful manipulations between the two tests. When pigeons are exposed to a weak, oscillating magnetic field between the two measurements, the analgesic response is inhibited and a hyperalgesic effect is recorded. The present findings are in agreement with previous studies showing that magnetic treatments may alter pigeons' emotional state and some of their behavioral patterns. © 1995 Wiley-Liss, Inc.
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    ISSN: 0197-8462
    Keywords: nociception ; tooth threshold ; skin threshold ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: To assess the effect of a magnetic treatment on pain perception, we compared the sensory threshold in 18 healthy volunteers. We determined the threshold by noninvasive electrical stimulation of the tooth pulp and skin before and after exposure to an altered magnetic field of low intensity and to a sham treatment. Five different parameters were recorded: the sensory and pain thresholds for the tooth and the sensory, pain, and tolerance thresholds for the skin. Two hours of exposure to a weak, oscillating magnetic field induced a significant decrease in three parameters (dental sensory and cutaneous pain and tolerance thresholds), whereas the other two parameters showed a similar tendency. When the same subjects were exposed to a sham treatment, only marginal, nonsignificant variations in all parameters were observed. These results represent the first piece of evidence that weak alterations of the magnetic field may induce hyperalgesia in humans. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 317-323 
    ISSN: 0197-8462
    Keywords: human performance ; statistical power ; parametric resonance theory ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two double-blind studies were run in an attempt to confirm the finding that a 0.2 Hz magnetic field affects simple reaction time (RT) in humans, whereas a 0.1 Hz field does not. In the first experiment, 12 volunteer subjects were exposed to a continuous 0.2 Hz, 0.1 Hz, or sham field in a fully counter-balanced, within-subjects design. Subjects were run singly for one condition each day over 3 consecutive days with a field strength of 1.1 mT and a daily exposure duration of 5 min. Neither magnetic field had any effect on RT at any time during the exposure. One condition of a second study, using a new group of 24 volunteer subjects, also failed to find any field effects at 0.2 Hz. Additionally, the second study failed to show any effects when the frequency, flux density, and field orientation were set according to parametric resonance theory. It is suggested that, although ELF magnetic field effects on human behaviour may be elusive, future research can improve detection rates by paying greater attention to reducing error variance and increasing statistical power. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 301-306 
    ISSN: 0197-8462
    Keywords: static electric fields ; EMF ; air ions ; taste-aversion learning ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Taste-aversion (TA) learning was measured to determine whether exposure to high-voltage direct current (HVdc) static electric fields can produce TA learning in male Long Evans rats. Fifty-six rats were randomly distributed into four groups of 14 rats each. All rats were placed on a 20 min/day drinking schedule for 12 consecutive days prior to receiving five conditioning trials. During the conditioning trials, access to 0.1 % sodium saccharin-flavored water was given for 20 min, followed 30 min later by one of four treatments. Two groups of 14 rats each were individually exposed to static electric fields and air ions, one group to +75 kV/m (+2 × 105 air ions/cm3) and the other group to -75 kV/m (-2 × 105 air ions/cm3). Two other groups of 14 rats each served as sham-exposed controls, with the following variation in one of the sham-exposed groups: This group was subdivided into two subsets of seven rats each, so that a positive control group could be included to validate the experimental design. The positive control group (n = 7) was injected with cyclophosphamide 25 mg/kg, i.p., 30 min after access to saccharin-flavored water on conditioning days, whereas the other subset of seven rats was similarly injected with an equivalent volume of saline. Access to saccharin-flavored water on conditioning days was followed by the treatments described above and was alternated daily with water “recovery” sessions in which the rats received access to water for 20 min in the home cage without further treatment. Following the last water-recovery session, a 20 min, two-bottle preference test (between water and saccharin-flavored water) was administered to each group. The positive control group did show TA learning, thus validating the experimental protocol. No saccharin-flavored water was consumed in the two-bottle preference test by the cyclophosphamide-injected, sham-exposed group compared to 74% consumed by the saline-injected sham-exposed controls (P 〈.0001). Saccharin-preference data for the static field-exposed groups showed no TA learning compared to data for sham-exposed controls. In summary, exposure to intense static electric fields and air ions did not produce TA learning as assessed by this particular design. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 9-10 
    ISSN: 0197-8462
    Keywords: Elf bioeffects ; Poisson processes ; threshold detection ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: I show that Pickard's mathematics cannot properly describe the effects of time-correlated signals in the presence of incoherent noise and cannot, therefore, properly address most signal-to-noise questions. Aside from this objection, I show that Pickard's conclusions follow from an ingenuous definition of “small signals” and do not hold for realistic models of biological processes and more conventional descriptions of “small signals.” © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 33-40 
    ISSN: 0197-8462
    Keywords: ELF ; calcium ion ; cyclotron resonance ; cell membrane ; ion channel ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The total current of Ca2+ ions through patch-clamped cell membranes was measured while exposing clonal insulin-producing β-cells (RINm5F) to a combination of DC and AC magnetic fields at so-called cyclotron resonance conditions. Previous experimental evidence supports the theory that a resonant interaction between magnetic fields and organisms can exist. This experiment was designed to test one possible site of interaction: channels in the cell membrane. The transport of Ca2+ ions through the protein channels of the plasma membrane did not show any resonant behavior in the frequency range studied. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 20-32 
    ISSN: 0197-8462
    Keywords: static magnetic fields ; magnetic field gradient ; adult mammalian neurons in vitro ; action potentials ; electrophysiology ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Notes: To characterize the inhibitory effect of a static magnetic field, action potentials (AP) were elicited by intracellular application of 1 ms depolarizing current pulses of constant amplitude to the somata of adult mouse dorsal root ganglion neurons in monolayer dissociated cell culture. During the control period, 〈5% of stimuli failed to elicit AP. During exposure to an ˜11 mT static magnetic field at the cell position produced by an array of four permanent center-charged neodymium magnets of alternating polarity (MAG-4A), 66% of stimuli failed to elicit AP. The number of failures was maximal after about 200-250 s in the field and returned gradually to baseline over 400-600 s. A direct or indirect effect on the conformation of AP generating sodium channels could account for these results because (I) failure was preceded often by reduction of maximal rate of rise, an indirect measure of sodium current; (2) recovery was significantly prolonged in more than one-half of neurons that were not stimulated during exposure to the MAG-4A field; and (3) resting membrane potential, input resistance, and chronaxie were unaffected by the field.The effect was diminished or prevented by moving the MAG-4A array along the X or Z axis away from the neuron under study and by increasing the distance between magnets in the XY plane. Reduction of AP firing during exposure to the ˜0.1 mT field produced by a MAG-4A array of micromagnets was about the same as that produced by a MAG-4A array of the large magnets above. The ˜28 mT field produced at cell position by two magnets of alternating polarity and the ˜88 mT field produced by a single magnet had no significant effect on AP firing. These findings suggest that field strength alone cannot account for AP blockade. © 1995 Wiley-Liss, Inc.
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    ISSN: 0197-8462
    Keywords: signal transduction ; Jurkat cells ; calcium concentration ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Notes: Low-frequency magnetic fields (MF) can increase the cytosolic calcium concentration ([Ca2+]i) in lymphocytes in the same manner as a physiological stimulus such as antibodies directed towards the CD3 complex. In this study, MF with various frequencies and flux densities were used, while [Ca2+]i changes were recorded using microfluorometry with fura-2 as a probe. The applied sinusoidal MF induced oscillatory changes of [Ca2+], in the leukemic cell line Jurkat in a manner similar to that seen with stimulation by antibodies. The response at 0.15 mT was over a frequency range from 5 to 100 Hz, with a fairly broad peak having its maximum at 50 Hz. The result of testing increasing flux densities at 50 Hz was a threshold response with no effect below 0.04 mT and a plateau at 0.15 mT. On the basis of the characteristic calcium pattern resulting from an applied MF, we suggest that MF influence molecular events in regular signal transduction pathways of T cells. © 1995 Wiley-Liss, Inc.
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  • 52
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    Bioelectromagnetics 16 (1995), S. 48-59 
    ISSN: 0197-8462
    Keywords: electromagnetic fields ; extremely low frequency ; case-control study ; static magnetic field ; magnetic resonance ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: We present a hypothesis that the risk of childhood leukemia is related to exposure to specific combinations of static and extremely-low-frequency (ELF) magnetic fields. Laboratory data from calcium efflux and diatom mobility experiments were used with the gyromagnetic equation to predict combinations of 60 Hz and static magnetic fields hypothesized to enhance leukemia risk. The laboratory data predicted 19 bands of the static field magnitude with a bandwidth of 9.1 μT that, together with 60 Hz magnetic fields, are expected to have biological activity. We then assessed the association between this exposure metric and childhood leukemia using data from a case-control study in Los Angeles County. ELF and static magnetic fields were measured in the bedrooms of 124 cases determined from a tumor registry and 99 controls drawn from friends and random digit dialing. Among these subjects, 26 cases and 20 controls were exposed to static magnetic fields lying in the predicted bands of biological activity centered at 38.0 μT and 50.6 μT. Although no association was found for childhood leukemia in relation to measured ELF or static magnetic fields alone, an increasing trend of leukemia risk with measured ELF fields was found for subjects within these static field bands (P for trend = 0.041). The odds ratio (OR) was 3.3 [95% confidence interval (CI) = 0.4-30.5] for subjects exposed to static fields within the derived bands and to ELF magnetic field above 0.30 μT (compared to subjects exposed to static fields outside the bands and ELF magnetic fields below 0.07 μT). When the 60 Hz magnetic fields were assessed according to the Wertheimer-Leeper code for wiring configurations, leukemia risks were again greater with the hypothesized exposure conditions (OR = 9.2 for very high current configurations within the static field bands: 95% CI = 1.3-64.6). Although the risk estimates are based on limited magnetic field measurements for a small number of subjects, these findings suggest that the risk of childhood leukemia may be related to the combined effects of the static and ELF magnetic fields. Further tests of the hypothesis are proposed. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 60-69 
    ISSN: 0197-8462
    Keywords: mobile phone ; AMPS ; dosimetry ; E-field ; eye ; brain ; temperature ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Electric fields (E-fields) induced within a phantom head from exposure to three different advanced mobile phone system (AMPS) hand-held telephones were measured using an implantable E-field probe. Measurements were taken in the eye nearest the phone and along a lateral scan through the brain from its centre to the side nearest the phone. During measurement, the phones were positioned alongside the phantom head as in typical use and were configured to transmit at maximum power (600 mW nominal). The specific absorption rate (SAR) was calculated from the in situ E-field measurements, which varied significantly between phone models and antenna configuration. The SARs induced in the eye ranged from 0.007 to 0.21 W/kg. Metal-framed spectacles enhanced SAR levels in the eye by 9-29%. In the brain, maximum levels were recorded at the measurement point closest to the phone and ranged from 0.12 to 0.83 W/kg. These SARs are below peak spatial limits recommended in the U.S. and Australian national standards [IEEE Standards Coordinating Committee 28 (1991): C95.1-1991 and Standards Australia (1990): AS2772.1-1990] and the IRPA guidelines for safe exposure to radio frequency (RF) electromagnetic fields [IRPA (1988): Health Phys 54:115-123]. Furthermore, a detailed thermal analysis of the eye indicated only a 0.022°C maximum steady-state temperature rise in the eye from a uniform SAR loading of 0.21 W/kg. A more approximate thermal analysis in the brain also indicated only a small maximum temperature rise of 0.034°C for a local SAR loading of 0.83 W/kg. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995) 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. I 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 70-74 
    ISSN: 0197-8462
    Keywords: video display terminals ; embryogenesis ; teratology ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Eighty-nine Swiss Webster mice were randomly divided into four groups: a control group, a pulsed magnetic field (PMF) group, a cytosine arabinoside (ara-C, a teratogen) group, and, a combined PMF + ara-C group. Mice in the PMF and PMF + ara-C groups were irradiated with a PMF (a sawtooth waveform with 52 μs rise time, 12 μs decay time, and 15.6 kHz frequency) at a peak magnetic flux density of 40 μT for 4 hours daily on days 6-17 of gestation. The mice in the ara-C and the PMF + ara-C groups were injected intraperitoneally on day 9 of gestation with 10 mg/kg of ara-C. The incidence of resorption and dead fetuses was not affected by PMF but was increased by ara-C injection. The malformation incidence of cleft palate (CP) and/or cleft lip (CL) was significantly higher in all three of the treated groups than in the control group (P 〈 0.05). If, however, statistical analyses had been done on litters rather than on individual fetuses, they would show that the incidence of CP and/or CL in the PMF group is not significantly greater than that in the control group. A significantly higher incidence of CP and/or CL was found in the PMF + ara-C group (49%) than the ara-C alone group (26.1%). These data suggest that PMF might enhance the development of ara-C-induced CP and/or CL. The incidence of minor variations in skeletal development, including reduction of skeletal calcification and loss of skeleton, was not statistically significant in the PMF group. However, it was higher in the two ara-C-treated groups, and there was no significant difference between the ara-C alone group and the ara-C + PMF group. From these results it is concluded that the very weak embryotoxic effects of PMF exposure may be revealed and enhanced in combination with a teratogenic agent. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 75-85 
    ISSN: 0197-8462
    Keywords: theoretical models ; nonlinear self-sustained oscillators ; limit-cycle oscillator ; intracellular calcium oscillations ; coherent fields ; noise ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Experiments on the effects of extremely-low-frequency (ELF) electric and magnetic fields on cells of the immune system, T-lymphocytes in particular, suggest that the external field interacts with the cell at the level of intracellular signal transduction pathways. These are directly connected with changes in the calcium-signaling processes of the cell. Based on these findings, a theoretical model for receptor-controlled cytosolic calcium oscillations and for external influences on the signal transduction pathway is presented. We discuss the possibility that the external field acts on the kinetics of the signal transduction between the activated receptors at the cell membrane and the G-proteins. It is shown that, depending on the specific combination of cell internal biochemical and external physical parameters, entirely different responses of the cell can occur. We compare the effects of a coherent (periodic) modulation and of incoherent perturbations (noise). The model and the calculations are based on the theory of self-sustained, nonlinear oscillators. It is argued that these systems form an ideal basis for information-encoding processes in biological systems. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 86-96 
    ISSN: 0197-8462
    Keywords: electromagnetic fields ; cancer ; currents ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: This study examines childhood cancer risk in relation to certain factors likely to indicate magnetic field exposure from ground currents in the home. Substantial ground currents are most often found in homes having conductive plumbing, in which an uninterrupted metallic path in the water pipes and water main connects the grounding systems of neighboring houses. Information on plumbing conductivity was obtained from water suppliers for the homes of 347 cases and 277 controls identified in an earlier study of magnetic field exposure and childhood cancer in the Denver area. An increased cancer risk was observed for children in homes with conductive plumbing: The matched odds ratio was 1.72 (1.03-2.88) and increased to 3.00 (1.33-6.76) when analysis was limited to cases and controls who were residentially stable from the reference date to the study date. A measurement metric likely to indicate active ground currents (measurements having above-median intensity and a nonvertical orientation of 〈55° from the horizontal) was identified. In contrast to measured field intensity alone, for which only modest associations with cancer have been reported, this metric shows a high and significant cancer risk [matched O.R. = 4.0 (1.6-10.0)] consistent over a range of intensity and angle cutpoints. Such elevated nonvertical fields were also associated with cancer in an independent data set, which was gathered to study adult nonlymphocytic leukemia in the Seattle area. The associations of cancer with conductive plumbing and with this exposure metric both suggest that cancer risk is increased among persons with elevated magnetic field exposure from residential ground currents. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 207-210 
    ISSN: 0197-8462
    Keywords: microwaves ; brain cells ; DNA damage ; rats ; single-strand ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: Levels of DNA single-strand break were assayed in brain cells from rats acutely exposed to low-intensity 2450 MHz microwaves using an alkaline microgel electrophoresis method. Immediately after 2 h of exposure to pulsed (2 μs width, 500 pulses/s) microwaves, no significant effect was observed, whereas a dose rate-dependent [0.6 and 1.2 W/kg whole body specific absorption rate (SAR)] increase in DNA single-strand breaks was found in brain cells of rats at 4 h postexposure. Furthermore, in rats exposed for 2 h to continuous-wave 2450 MHz microwaves (SAR 1.2 W/kg), increases in brain cell DNA single-strand breaks were observed immediately as well as at 4 h postexposure. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 211-215 
    ISSN: 0197-8462
    Keywords: ad/dc magnetic fields ; parametric resonance ; nociceptive ; land snails ; IPR model ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: Changes observed in the behavioral response of land snails from exposure to parallel ac and dc magnetic fields demonstrate limited agreement with the predictions of an interaction model proposed by Lednev and the predictions of a recently proposed ion parametric resonance (IPR) model. However, the inadequate number of reported data points, particularly in a critical exposure range, prevents unambiguous application of either the Lednev or the IPR model. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 231-240 
    ISSN: 0197-8462
    Keywords: ELF ; RF ; cell division time ; developmental effects ; mitosis ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: This study demonstrates that exposure to 60 Hz magnetic fields (3.4-8.8 mT) and magnetic fields over the range DC-600 kHz (2.5-6.5 mT) can alter the early embryonic development of sea urchin embryos by inducing alterations in the timing of the cell cycle. Batches of fertilized eggs were exposed to the fields produced by a coil system. Samples of the continuous cultures were taken and scored for cell division. The times of both the first and second cell divisions were advanced by ELF AC fields and by static fields. The magnitude of the 60 Hz effect appears proportional to the field strength over the range tested. The relationship to field frequency was nonlinear and complex. For certain frequencies above the ELF range, the exposure resulted in a delay of the onset of mitosis. The advance of mitosis was also dependent on the duration of exposure and on the timing of exposure relative to fertilization. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 241-249 
    ISSN: 0197-8462
    Keywords: electromagnetic field ; pulse modulati ; frog auricle ; twitch rate ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: Dependence of the microwave effect on modulation parameters (pulse width, duty ratio, and peak intensity) was studied in an isolated frog auricle preparation. The rate and amplitude of spontaneous auricle twitches were measured during and after a 2 min exposure to 915 or 885 MHz microwaves and were compared to preexposure values. The studied ranges of modulation parameters were: pulse width, 10-6-10-2 s; duty ratio, 7:100000, and peak specific absorption rate, 100-3000 W/kg. Combinations of the parameters were chosen by chance, and about 400 various exposure regimes were tested. The experiments established that no regime was effective unless the average microwave power was high enough to induce preparation heating (0.1-0.4°C). The twitch rate instantly increased, and the amplitude decreased, as the temperature rose; similar changes could be induced by equivalent conventional heating. The data provide evidence that the effect of short-term microwave exposure on the isolated heart pacemaker and contractile functions depends on pulse modulation just as much as modulation determines the average absorbed power. These functions demonstrated no specific dependence on exposure parameters such as frequency or power windows. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 270-271 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 263-267 
    ISSN: 0197-8462
    Keywords: magnetic field ; cholinergic neurons ; nerve growth factor ; mouse brain ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: C57/B1 mice were exposed during pregnancy (gestation days 0-19) to a 20 kHz magnetic field (MF). The asymmetric sawtooth-wave form magnetic field in the exposed racks had a flux density of 15 μT (peak to peak). After 19 days, the exposure was terminated, and the mice were housed individually under normal laboratory conditions. On postnatal day (PD) 1, PD21, and PD308, various neurochemical markers in the brains of the offspring were investigated and the brains weighed. No significant difference was found in the whole brain weight at PD1 or PD21 between exposed offspring and control animals. However, on PD308, a significant decrease in weight of the whole brain was detected in exposed animals. No significant differences were found in the weight of cortex, hippocampus, septum, or cerebellum on any of the sampling occasions, nor were any significant differences detected in protein-, DNA-level, nerve growth factor (NGF), acetylcholine esterase- (AChE), or 2′,3′-cyclic nucle-otide 3′-phosphodiesterase- (CNP; marker for oligodendrocytes) activities on PD21 in cerebellum. Cortex showed a more complex pattern of response to MF: MF treatment resulted in a decrease in DNA level and increases in the activities of CNP, AChE, and NGF protein. On PD308, the amount of DNA was significantly reduced in MF-treated cerebellum and CNP activity was still enhanced in MF-treated cortex compared to controls. Most of the effects of MF treatment during the embryonic period were similar to those induced by ionizing radiation but much weaker. However, the duration of the exposure required to elucidate the response of different markers to MF seems to be greater and effects appear later during development compared to responses to ionizing radiation. © 1995 Wiley-Liss, Inc.
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 284-289 
    ISSN: 0197-8462
    Keywords: mouse ; embryogenesis ; implantation ; resorption ; malformation ; magnetic field ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of low-frequency magnetic fields (MFs) on early pregnancy were studied in CBA/S mice. The magnetic field was a 20 kHz, 15 μT sawtooth. Pregnant females were divided into four groups, two control groups and two exposed groups. One group was exposed to MFs continuously from day 1 postconception (pc) until day 5.5 pc, and the other group was exposed continuously until day 7 pc. All animals were sacrificed on day 19 pc, the day before partus, and their uterine contents were analyzed. No significant increase in the resorption (early fetal death) rate was found in the exposed animals compared to the sham controls. In the group exposed during days 1.0-5.5 pc, the body weight and length of the living fetuses were significantly decreased. Except on day 3 pc (progesterone) and day 13 pc (calcium) in the treated groups, there were no significant differences in progesterone and calcium levels in peripheral blood. Implantation occurred on the same day in MF-treated and control animals. © 1995 Wiley-Liss, Inc.
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    Keywords: c-myc oncogene ; electromagnetic fields ; EBV-immortalized lymphoid cells ; HL60 leukemic cells ; synchronized cells ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of 50 Hz electromagnetic fields (EMFs) on the expression of the c-myc oncogene, known to be involved in normal cell proliferation and possibly also in tumor processes, were investigated in nonsynchronized human lymphoid cells immortalized by Epstein-Barr virus. Viral injury to such cells makes them a good model for exploring the possible cancer-promoting effects of 50 Hz magnetic fields. Parallel experiments were conducted on human HL60 leukemic cells. Cells were exposed to sinusoidal 50 Hz EMFs at 10 μT or 1 mT for 20 min, 1 h, 24 h, or 72 h. Exposure was performed either immediately after refeeding or 1.5 h after refeeding. C-myc transcript values were assessed by Northern blot analysis and normalized to those of the noninducible gene GaPDH. No statistically significant difference between the c-myc transcript levels of control and exposed cells was found in lymphoid or leukemic cells under our experimental conditions, either after short exposures of 20 min and 1 h or after longer exposures of 24 and 72 h. Other experiments were carried out with pseudosynchronized cells in an attempt to establish whether cells were especially sensitive to 50 Hz magnetic field exposure in any particular phase of the cell cycle. Accordingly, cells were pseudosynchronized in G0/G1 by serum deprivation and exposed for 20 min to a 50 Hz magnetic field, at 10 μT for lymphoid cells and 1 mT for HL60 cells. No significant difference was observed between the c-myc transcript levels of control and exposed cells for either of the synchronized cell types. These results for synchronized cells correlated with those for nonsynchronized cells. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 307-316 
    ISSN: 0197-8462
    Keywords: thermography ; temperature ; phantom ; dielectric properties ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: The possibility of tissue heating due to an auditory brainstem implant (ABI) or a modified cochlear implant (CI) during magnetic resonance imaging (MRI) of the head was tested on a full-sized human phantom using a realistic phantom head consisting of simulated skull, brain, and muscle. Dielectric properties of the brain, muscle, and bone materials were similar to those of human tissues at 64 MHz. The body consisted of homogeneous phantom muscle enclosed in a human-shaped fiberglass shell. Thermographic and fiber-optic temperature measurements were conducted to reveal any heating. Thermograms of sagittal, frontal, and horizontal planes of the head with the ABI and CI electrodes were taken immediately before and after a 26 min MRI scan. The MRI sequence was set at 94 excitations and 25 ms echo time to induce maximum radiofrequency heating, as suggested by the General Electric Company. The difference of these two thermograms gives the heating results. In two uncut phantom heads, Teflon tubes were placed along the implanted ABI and CI, and temperature data were recorded via fiber-optic probes before, during, and after the MRI. Results showed no observable heating associated with the ABI and the modified CI during worst-case MRI of the head. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 324-329 
    ISSN: 0197-8462
    Keywords: cytochrome P-450 ; mice ; rats ; Western blot ; immunology ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of 50 Hz, 1.2 mT magnetic fields (MFs) were tested on hepatic monooxygenase enzymes of basal and β-naphthoflavone-phenobarbital-preinduced rats and mice. An inductive effect on cytochrome P-450 level and on some enzymatic cytochrome P-450-dependent activities was observed in basal mice after MF exposure. Enzymatic activities in preinduced mice and rats were reduced by MFs, the degree of reduction depending on the enzyme. A specific inhibitory effect was determined in some of the assayed activities and in the relative peculiar P-450 isoforms detected by Western blot analysis. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 337-338 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 341-341 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 16 (1995), S. 119-123 
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    Keywords: EMF ; melatonin ; puberty ; sheep ; transmission line ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Topics: Biology , Physics
    Notes: In an earlier study, we found no effects of 60 Hz electric and magnetic fields (EMF) from a 500 kV transmission line on serum melatonin patterns or on puberty in ten female Suffolk lambs (Ovis aries). We conducted a larger replicate study of 15 lambs exposed to a mean electric field of 6.3 kV/m and a mean magnetic field of 3.77 μT and 15 controls exposed to EMF two orders of magnitude weaker than in the line area. The replicate produced essentially the same results as our previous study. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 113-118 
    ISSN: 0197-8462
    Keywords: amino acid ; hyperthermia ; magnetic resonance imaging (MRI) ; microwave ; monoamine ; radiofrequency radiation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: It is well known that metal objects perturb electromagnetic fields. Therefore, a conventional metal microdialysis probe cannot be used to determine the bioeffects of electromagnetic radiation. Using fused-silica tubing, we developed an inexpensive nonmetallic, rigid microdialysis probe for use in electromagnetic radiation research or during magnetic resonance imaging. This probe has a concentric tube design, with the membrane length adjustable to the size of the area to be dialyzed. The probes tested had regenerated-cellulose membranes that were 3 mm in length. This report describes how to make this probe. Average relative recovery rates at flow rates of 2.0, 1.0, and 0.5 μl/min were 21%, 27%, and 42%, respectively. These rates were slightly lower than the 30%, 42%, and 68% obtained with the commercially available metallic CMA10 microdialysis probe with a 3 mm membrane. This may be due to the fused-silica probe and CMA10 probe being made with different types of dialysis membranes. © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 405-405 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Bioelectromagnetics 16 (1995), S. 406-406 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 78
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    Bioelectromagnetics 16 (1995), S. 410-412 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 79
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    Bioelectromagnetics 16 (1995) 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 80
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    Bioelectromagnetics 16 (1995), S. 409-409 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
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  • 81
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 82
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    Bioelectromagnetics 16 (1995), S. 1-1 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 83
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    Bioelectromagnetics 16 (1995), S. 23-34 
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; method of constant stimuli ; psychophysics ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Because responses of animals to detection of the presence of an electric field (EF) are a possible mechanism for production of biological effects, it is important to know what EF intensities are detectable. Operant methods were used to train six baboons (Papio cyncocephalus) to perform a psychophysical task involving detection of EF presence. During the response phase of a trial, a subject responded on one push button to report the presence of the EF and on a different push button to report the absence of the EF. Correct reports of EF presence or absence produced delivery of food rewards. The subjects became proficient at performing this psychophysical detection task; during 35 days of testing, false alarm rates averaged 9%. The average EF detection threshold was 12 kV/m; the range of means among subjects was 5-15 kV/m. Two special test procedures confirmed that the subjects were responding directly to EF presence or absence and not to artifacts that might be associated with EF generation. The EF detection threshold of nonhuman primates is similar to thresholds reported for rats and humans. © 1995 Wiley-Liss, Inc.
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  • 84
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; electric field ; exposure ; homogeneity ; magnetic field ; stability ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A unique exposure facility was designed and constructed to generate large-scale vertical electric fields (EF) of up to 65 kV/m and horizontal magnetic fields (MF) of up to 100μT (1G), so that the behavioral and neuroendocrine effects of 60 Hz EF or combined electric and magnetic field (E/MF) exposure could be examined using nonhuman primates as subjects. Facility design and operational problems and their solutions are presented, and representative operational data from four sets of experiments are provided. A specially designed, optically isolated, 4 cm spherical-dipole EF probe and a commercially available MF probe were used to map the EF and MF within the fiberglass animal cages. In addition, amplifiers, signal conditioners, and A/D converters provided EF, MF, and transformer signals to a microcomputer at 15 min intervals. The apparatus produced homogeneous, stable E/MF at the desired intensities, and the fiberglass cages did not produce appreciable distortion or attenuation. Levels of recognized EF artifacts such as corona and ozone were negligible. The facility worked as intended, providing a well-characterized and artifact-controlled environment for experiments with baboons (Papio cynocephalus). © 1995 Wiley-Liss, Inc.
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    Bioelectromagnetics 16 (1995), S. 93-102 
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; interleukin 2 ; mitogen response ; peripheral blood lymphocytes ; T helper/suppressor cell ratio ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In a pilot immunology experiment, peripheral blood samples from six baboons (Papio cynocephalus) housed as a social group were collected during week 5 of preexposure, exposure, and postexposure periods that were each 6 weeks in duration. The subjects were exposed to vertical 6 kV/m and horizontal 50 μT (0.5 G) fields for 12 h per day. Lymphocytes collected during the exposure period displayed statistically significant (P 〈.05) reductions in CD3+ and CD4+ counts, interleukin 2 receptor expression, and proliferative response to pokeweed mitogen. A second experiment was conducted using samples from seven subjects exposed to 30 kV/m and 100 μT (1.0 G) and eight sham-exposed subjects. Statistically significant Period × Group interactions occurred for total white blood cell count and CD4+ to CD8+ ratio, but the pattern of results was not suggestive of an exposure-related effect. Although components of the nonhuman primate immune system appear to be affected by 60 Hz electric and magnetic field exposure in one of two experiments, additional experiments are required to evaluate this possibility. © 1995 Wiley-Liss, Inc.
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  • 86
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; computer-controlled ; intravenous catheter ; hormone ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: An automated blood sampling system was developed for use with tethered baboons (Papio cynocephalus) during concurrent exposure to 60 Hz 30 kV/m electric fields and 0.1 mT (1.0 G) magnetic fields. The system was controlled by a FORTH-based microcomputer, which operated a pump, a fraction collector, and two pinch valves. A swivel mechanism at the end of the tether allowed the baboons to move freely in their cages. The hardware and software were designed for fail-safe operation. Heparinized saline was infused at a rate of 0.5 ml/min until a sample cycle was initiated. Then, blood was drawn from the animal into a storage tube at a rate of 12.5 ml/min, a sample of undiluted blood was taken from the end of the storage tube near the baboon, and the blood remaining in the storage tube was then flushed back into the animal. Use of the storage tube prevented the peristaltic pump rollers from pressing on tubing containing blood, and return of the blood diluted with saline limited the blood wasted per sample to less than 0.5 ml. The system functioned reliably in three experiments, collecting samples as scheduled 97% of the time. Although it was initially designed for and used successfully with primates in an electric and magnetic field environment, this type of system could be employed in many areas of biomedical research or medical treatment. © 1995 Wiley-Liss, Inc.
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  • 87
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; cannula ; electric field ; magnetic field ; pineal gland ; radioimmunoassay ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Experiments conducted with laboratory rodents indicate that exposure to 60 Hz electric fields or magnetic fields can suppress nocturnal melatonin concentrations in pineal gland and blood. In three experiments employing three field-exposed and three sham-exposed nonhuman primates, each implanted with an indwelling venous cannula to allow repeated blood sampling, we studied the effects of either 6 kV/m and 50 μT (0.5 G) or 30 kV/m and 100 μT (1.0 G) on serum melatonin patterns. The fields were ramped on and off slowly, so that no transients occurred. Extensive quality control for the melatonin assay, computerized control and monitoring of field intensities, and consistent exposure protocols were used. No changes in nocturnal serum melatonin concentration resulted from 6 weeks of day-time exposure with slow field onset/offset and a highly regular exposure protocol. These results indicate that, under the conditions tested, day-time exposure to 60 Hz electric and magnetic fields in combination does not result in melatonin suppression in primates. © 1995 Wiley-Liss, Inc.
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  • 88
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; intermittent ; irregular ; pineal gland ; transient ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Experiments with rodents indicate that power-frequency electric field (EF) or magnetic field (MF) exposure can suppress the normal nocturnal increase in melatonin concentration in pineal gland and blood. In a separate set of three experiments conducted with nonhuman primates, we did not observe melatonin suppression as a result of 6 weeks of day-time exposure to combined 60 Hz electric and magnetic fields (E/MF) with regularly scheduled “slow” E/MF onsets/offsets. The study described here used a different exposure paradigm in which two baboons were exposed to E/MF with “rapid” E/MF onsets/offsets accompanied by EF transients not found with slowly ramped E/MF onset/offset; profound reductions in nocturnal serum melatonin concentration were observed in this experiment. If replicated in a more extensive experiment, the observation of melatonin suppression only in the presence of E/MF transients would suggest that very specific exposure parameters determine the effects of 60 Hz E/MF on melatonin. © 1995 Wiley-Liss, Inc.
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  • 89
    ISSN: 0197-8462
    Keywords: ELF ; magnetic fields ; cytotoxic effects ; male germ cells ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Flow cytometry (FCM) was performed to monitor the cellular effects of extremely-low-frequency magnetic field on mouse spermatogenesis. Groups of five male hybrid F1 mice aged 8-10 weeks were exposed to 50 Hz magnetic field. The strength of the magnetic field was 1.7 mT. Exposure times of 2 and 4 h were chosen. FCM measurements were performed 7, 14, 21, 28, 35, and 42 days after treatment. For each experimental point, a sham-treated group was used as a control. The possible effects were studied by analyzing the DNA content distribution of the different cell types involved in spermatogenesis and using the elongated spermatids as the reference population. The relative frequencies of the various testicular cell types were calculated using specific software. In groups exposed for 2 h, no effects were observed. In groups exposed for 4 h, a statistically significant (P 〈 0.001) decrease in elongated spermatids was observed at 28 days after treatment. This change suggests a possible cytotoxic and/or cytostatic effect on differentiating spermatogonia. However, further studies are being carried out to investigate the effects of longer exposure times. © 1995 Wiley-Liss, Inc.
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  • 90
    ISSN: 0197-8462
    Keywords: harmonics ; dosimetry ; numerical method ; ELF ; VLF ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Magnetic fields emitted by electric appliances such as razors, hair dryers, and drills were measured in the frequency domain. Results show the presence of high-frequency components (up to 96 kHz for razors, up to 3.4 kHz for hair dryers, and up to 8.6 kHz for drills) in the harmonic content of the fields. The measured fields were used to calculate the induced current densities in an anatomically based model of the human head (resolution 1.31 cm) by using the impedance method. The harmonic field contribution to the current density was higher than that from the carrier frequency for all the tested appliances. © 1995 Wiley-Liss, Inc.
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  • 91
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1995-09-29
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉New York, N.Y. -- Science. 1995 Sep 29;269(5232):1807.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11644753" target="_blank"〉PubMed〈/a〉
    Keywords: *Advisory Committees ; *Bioethical Issues ; *Bioethics ; Federal Government ; Genetics ; Government ; Human Experimentation ; *Public Policy ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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