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  • Articles  (372)
  • Blackwell Publishing Ltd  (372)
  • 1995-1999  (372)
  • 1965-1969
  • 1995  (372)
  • Process Engineering, Biotechnology, Nutrition Technology  (372)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nonparametric models for sensory discrimination methods are developed. In these models, the relationship between the probability of a correct response, Pc, and the measures for sensory discriminability or sensory difference, p1 and p2 are given. On the basis of the models, different sensitivities for triadic tests were compared including 3-AFC and triangle methods with the strong stimulus as the odd sample and with the weak stimulus as the odd sample. Predictions were made which can be tested experimentally.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A table is provided for testing the significance of R-index values. The use of the table is illustrated and the theory and assumptions behind the method are explained.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are many ways in which a laboratory difference test differs from ‘real life’ discrimination of foods. One of these is the interval of time between tasting the two stimuli to be discriminated. To investigate this, judges performed same-different discrimination tests using a citrus flavored beverage as a medium. The time interval between tasting the standard and comparison stimuli was varied. In this initial study, short intervals of zero, 30, 60 s were examined. For judges unfamiltar with the stimuli, performance deteriorated as the time interval increased. For judges familiar with the stimuli, the zero time interval elicited best performance but there was no decrease in performance when the interval was increased from 30 to 60 s. The results were explained by hypothesizing different types of memory trace for the standard stimulus being utilized for comparison with the comparison stimulus.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: To determine quality characteristics of snack chips, six snack chip prototypes containing cowpea flour, cornmeal, wheat flour and cornstarch (26, 34, and 42%) were formulated. Desirable characteristics as determined by three focus groups were a golden yellow color in a thin, smooth corn-chip form with crunchy or crispy texture, greaseless or dry mouthfeel and salty corn flavor. Chips were undesirable if brown-colored, thick, oily or greasy, had either hard or crumbly texture, and had bland, fishy, or burnt flavors. In consumer acceptance tests, commercial products were found to be more appealing than the experimental products. A discriminant model based on instrumental measurements of color and texture from 11 snack chip samples (six prototypes and five commercial samples) correctly grouped 100% of the acceptable and 90% of unacceptable products.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: To find selective and predictive tests in texture profiling, a series of 20 tests and 2 texture profiles were submitted to 25 subjects. Selection was carried out according to ranking, scoring and a texture property knowledge questionnaire. Two profiles were then done on 5 Bolognese sauce and 8 rice samples. Assessors were assessed by Spearman correlation coefficients for ranking tests, F values for scoring, average square canonical correlation coefficients for measuring the dimensionality of individual sample space, product discrimination and response repeatability for both Bolognese sauce and rice samples.Characterization test results showed that subject ability varied widely according to the test and the profile. Only one significant relationship between the texture property knowledge test and rice profile performances was found. More tests were successfully related to one or several texture attributes of both profiles. The ability to complete a complex profile could be discerned, albeit with difficulty, through simple selection tests.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Instrumental measurement of color places hue in a 360° continuum, but sensory description of color usually refers to distinct hues — red, green, yellow etc. Color evaluation of “Golden Delicious” apples by both an instrumental method and an experienced sensory panel was conducted to determine if sensory panelists perceive changes of golden apple color in green and yellow hues separately or on a hue continuum from green to yellow. Apple color changed from green to yellow during storage. Two different sensory scoring ballots were used to determine if sensory panelists view color in discrete green and yellow hues or on a green-to-yellow continuum. Data collected from this study demonstrated that sensory panelists can detect the color changes of decreasing greenness, increasing yellowness, and color change from green to yellow in an equal fashion. Hue angle represented the best predictor of sensory color perception.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Interindividual differences in sweet and bitter taste sensitivity were investigated using time-intensity (TI) measurements and multivariate statistics. TI profiles were obtained in triplicate from 25 subjects for 23 sweet and/or bitter stimuli first matched to be approximately equi-intense to 200 mM NaCl. Sweet stimuli, except for the larger sweeteners, were less persistent, and required less time to reach maximum intensity than bitter stimuli. The results of principal component (PCA) and cluster (CA) analyses of the stimuli X subjects matrices for maximum intensity (Imax), time to maximum intensity (Tmax), total duration (Tdur), and area under the curve (Area) suggest that sweet and bitter stimuli do not share common receptors; and that there are at least two receptor mechanisms each for sweet taste (one for sugars and other small compounds, and the other for large sweeteners) and bitter taste (one for PTC/PROP and one for other bitter compounds).
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objective of this study was to assess the sensory characteristics of potassium lactate (PL) in combination with salt (NaCl) in a model system at common usage levels for meat systems. Using a gelatin-based (3%) model system containing various concentrations of potassium lactate (0%, 1%, 2%, and 3%) and sodium chloride (0%, 0.5%, 1%, 1.5%, 2%, 2.5%, and 3%), a sensory panel (10) selected for salt sensitivity (0.08% threshold or less) scored the intensity of saltiness and bitterness of each treatment combination using a scale of 0 = none and 15 = intense. As salt concentration increased, saltiness increased and bitterness decreased; as PL increased, saltiness and bitterness increased. At 2% salt, the bitterness of PL was masked; however, salt concentrations below and above 2% were less effective in bitterness reduction.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Multidimensional scaling (MDS) procedures produce maps of stimulus points, the dimensions of which may be sensory and/or physicochemical attributes. Our objective was to test the efficiencies of a cyclic design and a MDS method in the study of saltiness perception. Two levels of NaCl (0.1% and 0.2%, w/v) were added to two nonionic and two ionic gum solutions prepared to two concentrations. Subjects scaled dissimilarities between pairs of solutions and rated each sample for flavor attributes. Solution pairs were selected using cyclic designs. Repeated measures ANOVA determined that added NaCl was the only significant factor affecting saltiness perception. In contrast, from KYST-2A MDS maps, we concluded that saltiness perception was influenced by gum property, gum concentration, presence of calcium and potassium, and related to binding of the sodium ion (Na+) as determined by23Na nuclear magnetic resonance (NMR) spectroscopy. The MDS cyclic design successfully reduced the number of samples and subjects when using experienced subjects.
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  • 11
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    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Forty-three samples of shellfish (22 mussels and 21 clams) purchased from retail outlets from February through October were tested for the presence of vibrios associated with human disease. Vibrio spp. was found in 51.16% of the samples. V. alginolyticus was the commonest Vibrio species found in the samples, followed by V. parahaemolyticus, V. fluvialis and V. cholerae non-01. All V. parahaemolyticus isolated were Kanagawa-phenomenon negative. Hemolytic activities were shown in all isolates of V. fluvialis and V. cholerae. Bacterial indicators of quality and safety were within permitted limits by authorities. The results indicate the potential risks of food poisoning associated with the consumption of raw or undercooked shellfish.
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Meat and meat products are important vehicles of foodborne illness outbreaks in European countries. Salmonellas were the most commonly reported aetiology of infection, although the relative importance of other agents varied. The factors contributing to the increase in food poisoning and salmonellosis in England and Wales related both to foods eaten and their preparation. The implication of foods of animal origin as principle vehicles of infection was strengthened by reports associating these foods with outbreaks of human illness, and reports of salmonella infection in animals and poultry. The current increase in salmonella infection associated with poultry products suggests that reducing infection in, or contamination of poultry could significantly decrease human illness.The problem of human salmonellosis is multi-factorial. Trends are driven by both intrinsic factors relating to the microbiological quality of the food and standards of preparation, and extrinsic factors, such as ambient temperature, which amplify the intrinsic effects. Many of these factors may be amenable to preventive activities, including programs to reduce infection in animals and poultry and programs to educate the consumer in safe food handling.The costs of human salmonella infection in England and Wales were estimated to be between £231 million and £331 million in 1988 of which £143 million to £205 million may have been associated with meatborne infection. Reductions in human illness, as modelled by irradiation of poultry meat, could give substantial economic gains.
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  • 14
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Many chemical and microbial indicators can be used to determine the safety and shelf-life of meats. This paper considers their potential application as elements in an effective HACCP system. A range of chemical indicators, including lactic and acetic acids and citrulline are examined. Many of these are of limited value, although citrulline offers some potential. Microbially based indicators and systems including, a contamination index and the use of temperature function integration are discussed. The correlation to the growth of pathogens is outlined, with particular reference to the modelling of E. coli. The potential value of psychrotrophic and psychrophilic counts as process hygiene indicators in the prediction of meat and keeping quality is elucidated. The application of these systems in HACCP is presented, with particular emphasis on their value in monitoring and verifying critical control points. The value of developing more effective methods using rapid techniques in this area is highlighted.
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The use of DNA probes for rapid identification of foodborne pathogens is an emerging field. We have standardized a method for the detection of Salmonella in food samples by using a specific insertion sequence (IS-200) of Salmonella chromosomal DNA as the probe. The procedure for colony hybridization, and washing conditions for the stringency were established. All the ten Salmonella strains, belonging to various serotypes tested were positive while 18 other bacterial cultures, mainly belonging to the family Enterobacteriaceae such as E. coli Shigella, Proteus etc., were negative in colony hybridization. The sensitivity of the detection was found to be 105 cfu using 32P-labeled probe. We surveyed 16 chicken and 6 fish samples from local market using IS-200 probe after preenrichment and selective enrichment. All the samples tested were found positive with DNA probe. The major strains of Salmonella isolated from these samples were S. gallinarum, S. typhimurium, S. enteritidis, S. choleraesuis and S. paratyphi A. These results were confirmed by standard Salmonella identification method.
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Precooked homogenized crawfish tail meat was inoculated with Listeria monocytogenes, treated with lactic acid, and packaged in various atmospheres for D value determinations at 60C. D60 values using 0.0, 0.5, 0.75, and 1.0% lactic acid (LA) treated meat were 4.68, 4.41, 3.46, and 2.49 min, respectively. LA increased sensitivity of L. monocytogenes to heat but this was not solely due to a reduction of pH. Sensory analyses of odor, color, taste, and texture of uninoculated whole crawfish tail meat demonstrated no significant differences (P 〉 0.05) between LA treated samples and controls. D60 values for the bacterium in crawfish tail meat homogenate packed in air, O2, CO2, or N2 were 10.58, 9.22, 11.68, or 12.97 min, respectively, which were not significantly different (P 〉 0.05). When 1% LA was added, significant decreases (P 〈 0.05) in D60 values were observed in each packaging atmosphere. Similar (P 〉 0.05) D60 values for crawfish tail meat treated with 1% LA and packed in air, O2, CO2, or N2 were 3.44, 3.88, 3.48, or 3.08, respectively. These results show that heat treatments of 60C were lethal to L. monocytogenes in packaged crawfish tail meat homogenate regardless of package atmosphere. Additionally, LA increased the lethality of heat to the bacterium in all tested package atmospheres.
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  • 17
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of chlorine against 2 strains of E. coli attached to the surface of beef carcass tissue (BCT) were examined using a model carcass washer. Lean and adipose BCT with approximately 5 log10CFU/cm2 E. coli bacteria were spray-treated with water and sodium hypochlorite (NaOCl) to give chlorine concentrations of 50, 100, 250, 500, or 800ppm, incubated for 24 h, 4C, and E. coli populations enumerated. Spray treatments with water did significantly (P 〈 0.05) reduce the bacterial populations of either organism attached to lean or adipose BCT, as compared to populations of controls; however, reductions were less than 0.60 log10CFU/cm2. Treatments with 500 and 800 ppm chlorine against E. coli ATCC 25922 attached to BCT resulted in the greatest reductions of 1.22 and 1.28 log10CFU/cm2, respectively. At 800 ppm chlorine, E. coli O157:H7 ATCC 43895 attached to BCT was reduced by 1.04 log10CFU/cm2, whereas spray treatments with 50, 100, 250, and 500 ppm chlorine resulted in reductions of 〈 1 log10CFU/cm2. Spray treatments with chlorine from sodium hypochlorite solutions reduced populations of E. coli, however, these reductions were not sufficient to completely inactivate the bacteria attached to red meat.
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  • 18
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The triangular method, the same-different rating method, and the standard same-different method were each used with 10 tasters to obtain estimates of the discriminability of two concentrations of a colorless beverage. There were no significant differences in d′, an index of discriminability, among the three tasks. In the light of this parity, a choice amongst the methods should be driven by practical considerations. When receiver operating characteristics (ROCs) were fitted to the rating data, the model underlying published tables of d′ was strongly supported, so that discriminability can be assessed from same-different data either by entering tables or by fitting ROC curves. Same-different methods appear more acceptable to tasters than the triangular method.
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  • 19
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: paungency is a physical sensation usually perceived as a component of flavor. Foods and beverages which are described as pungent often impart unpalatable sensations to those uninitiated in the experience. Variations in pungency were examined in whiskies, ethanol-water mixtures, and whisky model systems. Effects on sensory pungency of changes in composition showed that pungency in whisky is not only dependent on the concentration of ethanol in the headspace, but also on a complex of other effects.
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  • 20
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Time-Intensity Curves are often summarized by average curves. From these curves TI-parameters are inferred. In this paper a different method is proposed in which the shape of the curves is the central concept. Isotropic scaling factors are computed for each curve. These factors can be tabled and inspected. Often a large number of scaling factors will be computed, which makes it hard to study them in a table. In such cases they can be represented in a plot, for which in this paper PCA is suggested. In this plot a structure in the scaling factors for the different TI-curves may become visible. It is also possible to make a plot in which the homogeneity of the panel of subjects can be studied.
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  • 21
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Texture of orange gels prepared with 15% fruit pulp, sucrose up to 55° Brix and five different gelling agents — kappa-carrageenan, kappa-carrageenan plus locust bean gum, alginate, gellan gum, and gellan, xanthan and locust bean gums — was studied by Free-Choice Profile (FCP) analysis. Maximum rupture force and deformation at rupture were also determined by uniaxial compression in an Instron texturometer. Generalized Procrustes Analysis applied to FCP data permitted differentiation between samples and informed on the textural attributes responsible for the observed differences. Sensory differences were in general in accordance with mechanical differences. However, carrageenan and gellan gum gels were differentiated with the sensory method applied but not with mechanical tests.
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  • 22
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    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In order to evaluate the suitability of signal detection theory methods for assessing the discriminability of foods and beverages, the discriminability of two dairy milk products that differed in fat content was measured with two detection-theoretic methods: the single-interval rating method, and the same-different method. The nominal fat contents of the milk products were 0.1 and 1.6%. Measures of discriminability for three observers were derived by fitting receiver operating characteristics (ROCs) based on equal-variance normal models to the ratings of each observer with a procedure that combined jackknifing and maximum-likelihood estimation. The fitted ROCs provided a good fit to the data indicating that the equal-variance models were appropriate for these tasks. The best-fitting estimates of d′ obtained for each task were not significantly different, demonstrating that d′ is a measure of sensitivity that is largely independent of the task from which it is determined. However, estimates of proportion correct obtained for each task were shown to be significantly different.
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  • 23
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method for the first step in the selection of trained sensory assessors, based on their skill in identifying basic tastes in low concentration solutions, was defined and evaluated. The solutions used were: 0.4 and 0.8% sucrose; 0.03, 0.04 and 0.06% citric acid; 0.08 and 0.15% sodium chloride; 0.02 and 0.03% caffeine. Concentration ranges were found adequate. The criterion for selection was to have over 65% correct answers. Out of 226 candidates who received the test, 141 (62%) were selected. The probability of a candidate having more than a certain percentage of correct answers was calculated. The influence of the dilution water quality on the perception of basic tastes was significant; distilled water was the most adequate.
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  • 24
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In a recent paper Berglund et al. (1993) applied the triangle task in conjunction with economic incentives to assess the bias and sensitivity of panelists discriminating between food and beverage products. A re-examination of the data from that study casts doubt on their interpretation of the results. Detection theory analyses of performance in sensory difference tests are increasingly used in food science and, where appropriate, permit independent bias and sensitivity measures to be extracted from discrimination data without the additional costs associated with the economic incentives test.
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  • 25
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The microbiological status of beef carcass meat was investigated at a traditional and a semi-modern abattoir in Indonesia. Carcasses from the traditional abattoir were generally less contaminated than those from the semi-modern abattoir. The average total aerobic viable bacterial count of carcasses produced at the traditional abattoir, (log10 3.62/cm2) was found to be significantly lower (p = 0.02) at the semi-modern abattoir (log10 4.02/cm2). More than 70% of carcasses at both abattoirs were found to be positive for Escherichia coli (〉 10 organisms/cm2), while Salmonellae were found on approximately 15% of carcasses at both locations.
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  • 26
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Characterization of ten Aeromonas spp. isolates from local foods and A. hydrophila ATCC 7965, revealed that all strains of A. hydrophila, A. sobria and one strain of A. caviae showed strong hemolytic activity. All of them were protease producers except one A. caviae strain. Some A. hydrophila and A. caviae strains fermented lactose, coagulated milk and reduced litmus. The majority of strains were resistant to at least 5 of 14 antibiotics tested. All the strains grew over the range of 5–42C except those of A. caviae which failed to grow at 42C. Frozen storage at -16C greatly decreased the viability of the tested strains. Radiation D10values of strains ranged from 0.109–0.253 kGy in nutrient broth and increased when some strains were irradiated in ground meat at room temperature. Both strains of A. hydrophila (ATCC andFI), A. sobria FI and A. caviae FI grew normally in the presence of 1 and 3% salt. Only A. hydrophila FI tolerated 6.5% salt. These four strains were able to grow in the presence of 0.05–0.3% potassium sorbate at pH 7.0, while at pH 5.0 the presence of sorbate prevented their growth.
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  • 27
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Acetanisole (4′-methoxyacetophenone) (AC), benzaldehyde (BE), cinna-maldehyde (CI), diacetyl (DI), phenylpropionaldehyde (PH), and pyruvaldehyde (PY) were tested against growth of S. aureus WRRC B124 in brain heart infusion broth. Activity was assessed in the presence and absence of oxygen at 12, 19 and 37C, and in combination with mild (20 min at 50 or 60C) heat treatments. The carbonyl compounds limited S. aureus growth at minimal inhibitory concentrations (MIC) of 〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:01496085:JFS337:les" location="les.gif"/〉 0.5–8.0 mM. After 4 h at 37C, a 2 to 3-log10 CFU/ml population reduction was observed with cultures containing 8.0 mM PH, PY, DI or CI. Activity was O2-tension independent, with CI (0.5 mM), DI(2.0 mM) and PY(2.0 mM) being most active. The MIC for CI was temperature independent, while PY was most effective at 19C, and PH and DI at 12C. Mild heat treatment of carbonyl-supplemented samples reduced previously observed MICs. At 60C, for example, the MICs for AC and BE, 4.0 and 8.0 mM respectively, were both reduced to 〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:01496085:JFS337:les" location="les.gif"/〉 0.5 mM. The decimal reduction times for S. aureus exposed to both UV-light and 8 mM flavor compounds were 3.3 and 4.3 s for CI and DI, respectively. However, the other compounds were not as effective in the presence of UV since the decimal reduction times ranged from 7.7 to 9.0s. The carbonyl compounds tested were effective antistaphylococcal agents and their use in combination with thermal processing may serve as a new approach to control S. aureus growth and other gram-positive foodborne pathogens.
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  • 28
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 29
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Meat products are very important sources of protein in the human diet. The contamination of these products with pathogenic microorganisms, such as Salmonella and Campylobacter, make both production and consumption of them a precarious proposition. Several methods can decrease the level of contamination with these pathogenic microorganisms. However, there are still situations of high microbial load which cannot be explained and are often attributed to stress. This paper describes the effect of transport, husbandry and nutrition practices on contamination of slaughtered products. Examples of the stress occurring during fattening, catching and loading, transport and conditioning at the processing plant, are given.
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  • 30
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 31
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effectiveness of decontamination systems for controlling the presence of pathogens and spoilage organisms on carcasses is discussed. Research using organic acids and water (hot or cold) demonstrates the general effectiveness of such treatments in lowering the aerobic plate counts (APC) on carcasses by 1–3 log10 cfu per unit area. Chlorine has been found to be relatively ineffective for use in animal carcass spray washers. An example of direct application of the bacteriocin nisin to inoculated tissue in a spray washer is discussed. Reductions attributable to nisin were 2–2.5 log10 cfu per unit area higher than those reported for various organic acids or water. Areas for further research are highlighted along with the potential use of newer technologies to elucidate attachment and detachment mechanisms of bacteria to meat animal carcasses.
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  • 32
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The individual and combined antilisterial activity of the preservatives sodium lactate (4%), potassium sorbate (0.3%) and nisin (400 IU/ml), in either the presence or absence of the curing salts nitrite (125 ppm) and polyphosphate (0.5%), was assessed in buffered BHI broth (pH 5.5) during incubation at 4C. A cocktail of the strain Listeria monocytogenes NCTC 7973 and two food derived strains was used as the inoculum in challenge studies. In the absence of the preservatives, L. monocytogenes grew in the presence of polyphosphate but not in the presence of nitrite or a combination of nitrite and polyphosphate. The antilisterial action of nitrite was predominantly bacteriostatic in nature. Lactate acting alone, or in the presence of curing salts, produced a bacteriostatic effect. Sorbate acting alone had a bacteriostatic effect. Sorbate acting in the presence of nitrite or nitrite and polyphosphate produced a marked listericidal effect, reducing the population by 6.7 logs and 5.4 logs, respectively. Nisin acting alone produced an almost immediate 90% listericidal response (1 log population decrease), but initial numbers were restored within 14 days by regrowth. Regrowth was eliminated when nisin was used in combination with lactate, nitrite or nitrite and polyphosphate. The combination of lactate and sorbate offered no advantage over the use of sorbate alone. Sorbate and nisin acting in combination produced an enhanced listericidal effect, which was also seen in the presence of curing salts, but was delayed. No listericidal advantage over sorbate-nisin combination was achieved through the use of the lactate-sorbate-nisin combination. The combined use of sorbate and nisin offers promise as a means of eliminating L. monocytogenes from low pH cured meat products. The efficacy of this preservative combination remains to be evaluated in a meat system before its practical application can be considered.
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  • 33
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Five strains of Cellulomonas (ATCC 482, ATCC 488, ATCC 491, ATCC 15724 and ATCC 21399) were evaluated for toxicity. Extracts of cells in both water and oil as well as the cell free spent broth injected into fertile chicken eggs were not lethal to the embryos. Viable organisms inoculated in rats, mice and rabbits by oral, subcutaneous and intraperitoneal routes respectively, did not cause generalized or localized infection nor abnormal changes in the vital organs. The sexual maturity, fertility and the vital organs of adult rats as well as the vital data of their progeny were not affected by feeding 10% protein from all strains.
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  • 34
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cylindrical samples of beef semitendinosus muscle were dipped into an inoculum of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium. After air drying for 10 min, samples were sanitized by dipping in fumaric acid (1.0 or 1.5%), lactic acid (1%) or acetic acid (1.0%) solutions for 15 or 30 s at 55C. The 1.5% fumaric acid was most inhibitory against L. monocytogenes, resulting in inactivation of this pathogen after 7 days of storage. Samples treated with 1.0% and 1.5% fumaric acid solutions for 30 sec gave 2.54 and 1.51 log10 reductions, respectively, in S. typhimurium population compared to nontreated samples. The 1.5% fumaric acid treatments for 30 s exerted the maximum inhibition of E. coli O157:7. Acetic and lactic acid treatments were significantly less effective in reducing populations of the tested pathogens than fumaric acid treatments. As the sanitizing time in acid solutions increased from 15 to 30 s, the numbers of surviving organisms were reduced significantly.
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  • 35
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    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Contaminants are capable of growth on meat and poultry products due to the nutrient composition of the products, the available water, the favorable pH and very often the temperature and other conditions of storage. These factors and processing conditions influence greatly the pattern of development of the spoilage and pathogenic microflora. This paper will consider the effects of the general bacterial flora on pathogenic bacteria occurring on meat and poultry as well as the impact of external factors on some of the different types of bacteria found on these products.
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  • 36
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The concept of “Healthy Food from Healthy Animals”relies upon the pre-and postharvest implementation of an integrated approach for protecting the food product, from contamination or adulteration with harmful agents. This extended form of control is now an integral part of Hazard Analysis Critical Control Point Programs and provides a practical means of validating the health status of herds and flocks of food-producing animals. In this context the veterinary food hygienist, as a food company employee, has much to offer the agri-food industry. She/He can appraise not alone the onfarm conditions but also the animal health and other data provided by the regulatory veterinarian assigned to the plant. Such information, complemented by the plant's own food quality and safety assurance program, can then be used to improve and promote the safety standards of the company. Extended health control programs emphasize the health and safety of the final product for the consumer at each stage of production and processing, and enable veterinary food hygienists, and others, to provide the agri-food industry, and governmental agencies, with a scientific basis for harmonizing trade in meat and other animal-derived foods.
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  • 37
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An important component of a Hazard Analysis Critical Control Point (HACCP) approach applied to animal production is reducing the carriage of food-associated pathogens by animals. Two approaches that have both great potential for reducing pathogen colonization of animals and merit for practical application include (1) vaccination and (2) competitive exclusion. Vaccination involves acquisition of immunity in an animal following exposure to an attenuated pathogen or an antigen of a virulent microorganism. Adherence factors that influence bacterial colonization of animals are useful antigens for vaccines. A strategy to developing a vaccine to reduce carriage of pathogens includes (1) identifying sites of colonization by the pathogen in the animal, (2) defining the mechanism of colonization, (3) characterizing genes that encode colonization factors, (4) transforming the colonization factor antigen genes into a suitable nonpathogenic vector, and (5) determining the optimal methods of immunization. Studies are underway to identify colonization factors of Escherichia coli O157:H7 for use in vaccine development to protect cattle from colonization by this pathogen. Competitive exclusion involves the use of microbial cultures that out-compete pathogens from colonizing specific niches. A science-based approach to identifying/developing useful competitive exclusion cultures is to: (1) define how a pathogen colonizes the site of interest, (2) isolate microbes that colonize the same site and produce metabolites that inhibit or kill the pathogen, and (3) verify that the inhibitory microbes, when introduced into pathogen-free animals, can reduce or prevent colonization by the pathogen. This approach has been used successfully to identify defined bacterial cultures that can greatly reduce colonization of Campylobacter jejuni in poultry. Commercial implementation of techniques of these types is an essential part of the overall HACCP approach to reducing the prevalence of foodborne pathogens from farm to fork.
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  • 38
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Clostridium perfringens growth from a spore inoculum was investigated in vacuum-packaged, cook-in-bag ground beef that included 0.3% (w/w) sodium pyrophosphate, pH 5.5 or 7.0, and salt (sodium chloride) at 0 or 3% (w/w). The packages were processed to an internal temperature of 71.1C, ice chilled and stored at various temperatures. The total C. perfringens population was determined by plating diluted samples on tryptose-sulfite-cycloserine agar followed by anaerobic incubation for 48 h at 37C. At 28C, a combination of 3% salt and pH 5.5 was effective in delaying growth for 24 h. At 15C, growth occurred within 6 days in samples with pH 7.0, but was delayed until day 8 in the presence of 3% salt at pH 7.0. Vegetative cells were not observed even after 21 days 21 of storage at 15C in the presence 3% salt at pH 5.5. C. perfringens growth was not observed at 4C regardless of pH or salt levels. The D-values ranged from 23.33 min (3% salt, pH 7.0) to 13.99 min (3% salt, pH 5.5). Cyclic and static temperature abuse of refrigerated products for 12–15 h did not permit C. perfringens growth. However, temperature abuse of cook-in-bag beef products for periods longer than 15 h in the absence of salt led to growth of C. perfringens from a spore inoculum.
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  • 39
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Several organic acids or salts approved as food additives enhance the inactivation of foodborne pathogens such as Listeria monocytogenes. Although there has been research on the effects of individual organic acids on the inactivation kinetics of L. monocytogenes, little information exists on their activity when used in combination with other food additives. We undertook to characterize the effects of combinations of 90% sodium acetate or sodium propionate, two salts that inhibit L. monocytogenes, with 8% EDTA (disodium salt) and 2% ascorbic acid on the nonthermal inactivation of a three-strain mixture of L. monocytogenes. Activity was assessed in Brain Heart Infusion broth (BHI) at various concentrations (0.0–2.0% w/v), pH values (3.0–4.5) and temperatures (4–28C). Samples were removed periodically for up to 175 days and viable counts determined. Survivor curves were generated using a logistics-based inactivation model and used to calculate “time to a 4-D (99.99%) inactivation”(t4-D). The rate of inactivation was directly related to concentration of the acid mixture and temperature of incubation and inversely related to pH. The primary factor effecting inactivation rates was pH, followed by the concentration of the undissociated form of the primary organic acid (acetic or propionic). Evaluation of the mixture components individually and in combination indicated the components acted largely in an additive manner. The results indicate that combinations of primary and secondary organic acids and EDTA may have advantages for enhancing the inactivation of L. monocytogenes in refrigerated, mildly acidic foods, while avoiding organoleptic effects associated with excessive levels of single acids.
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  • 40
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    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sensory analysis has traditionally played a prominent role in quality control for food products. More and more principles from sensory analysis are also applied in the area of food product development, bringing sensory analysis more closely into the domain of marketing. Unfortunately, in practice integration between sensory and marketing practices is far from optimal. Differences in basic orientations between sensory and marketing are a major source contributing to this defective cooperation.Sensory analysis has traditionally been product oriented with an emphasis on internal validity of the test results. Implicitly or explicitly this approach emphasizes the relationships between sensory evaluation and characteristics of the product. Marketing, on the other hand, stresses the external validity of test results: the extent to which test results can be generalized to market behavior. Emphasis on external validity requires an approach to sensory analysis that is fundamentally different from current sensory practice in terms of type of respondents, type of stimuli, scaling procedures adopted and test circumstances.Closer integration between the product and consumer oriented approach to sensory analysis may contribute to the success of product development in the food industry. The literature suggests several factors that may contribute to a more fruitful cooperation between the two approaches to sensory analysis. The company's senior management plays a central role in the achievement of this integration by providing an infrastructure (in terms of personnel, organizational structure and financial resources) that paves the way for closer cooperation.
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  • 41
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Listeria monocytogenes was inoculated onto fresh pork and turkey slices. Inoculated and control samples were packaged under modified atmospheres (100% N2, and 20%/80% and 40%/60% CO2O2) or air in plastic bags of low gas permeability. Samples were stored at 1 and 7C. Samples stored in air showed a similar microbiological pattern to that usually observed in fresh meat stored aerobically. Packaging under modified atmospheres extended the meat shelf-life. Bacterial growth was strongly inhibited at 1C, particularly in samples stored under CO2/O2 enriched atmospheres. Temperature and pH were critical factors for L. monocytogenes growth. This pathogen grew only on pork (initial pH 5.3) packaged in air and stored at 7C. No L. monocytogenes growth was observed at 1C in any atmosphere assayed. However, growth on turkey (initial pH 6.0) was marked at 7C in all atmospheres tested, while at 1C, this bacterium grew weakly only on samples stored in air.
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  • 42
    ISSN: 1745-4565
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects and interactions of heating temperature (70–90C), pH (5–6.5), sodium chloride (0–3%), and sodium pyrophosphate (0–0.3%) on the heat resistance of a six strain mixture of spores of nonproteolytic Clostridium botulinum type B and type E in turkey were examined. Thermal death times were determined in submerged vials heated using a water bath. Heated spores were recovered on Reinforced Clostridial Medium (RCM) supplemented with lysozyme (10 μg/ml). Decimal reduction times (D-values) were calculated by fitting a survival model to the data with a curve fitting program. The D-values were analyzed by second order response surface regression for temperature, pH, salt (sodium chloride) and sodium pyrophosphate levels. The four variables interacted to effect the inactivation of spores. Confidence intervals (95%) predicted heat resistance of spores in turkey. The data suggest that the effect of reduced pH in increasing the inactivation was more pronounced at high temperatures and may provide an adequate degree of protection from nonproteolytic C. botulinum spores in minimally processed foods, particularly if employed in conjunction with combinations of salt and sodium pyrophosphate.
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  • 43
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 44
    ISSN: 1745-4565
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Temperature abuse is a main concern for chilled and/or MAP meat and poultry products since it will not only cause economic loss, but may also lead to a foodborne illness hazard. A major question for such products is whether organoleptic spoilage due to microbial or chemical action will occur before pathogen numbers or toxin levels become a risk when a product undergoes abuse temperatures. Time-temperature integrators (TTI) have shown potential to indicate or estimate temperature abuse. However knowledge of predictive food microbiology is needed for each food to employ a TTI tag. The simple Monod and the nonlinear Gompertz function can be used to obtain growth parameters (i.e., lag time, exponential growth rate) from kinetic studies of microbial spoilage. The temperature dependence of these parameters is best evaluated by the Arrhenius or the square root model. Effects of temperature on other deteriorative processes can also be described similarly. Three approaches are presented to evaluate the extent and impact of temperature abuse on meat and poultry by use of TTI tags, which include the effective temperature, the equivalent time and the equivalent point approach. Other technologies to evaluate or to reduce the impact of temperature abuse are also discussed.
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  • 45
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    Journal of food safety 15 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 46
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The United States Department of Agriculture's Food Safety and Inspection Service has initiated the National Microbiological Baseline Data Collection Programs to determine the prevalence and levels of selected bacteria on meat and poultry slaughtered under federal inspection. This paper reviews the methodology and results of the first completed study on samples collected from steer and heifer carcasses. Potential applications for this type of testing are discussed.
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  • 47
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The current annual risk of acquiring a foodborne disease in the United States is estimated at 2.7 × 10-2. The risk of associated death is estimated at 3.7 × 10-5. These represent a health care burden 〉$3 billion. Using a risk assessment model one can identify levels of microbial contamination which may be unacceptable in foods and appropriate controls needed to reduce these levels. Salmonella bacteria continue to represent a large percentage of the identifiable infections. A model developed from human dose-response studies predicts the probability of infection for Salmonella at 7.5 × 10-3 with exposure to a single CFU of the organism. Risks of severity (hospitalization), mortality, reactive arthritides, and mortality in the elderly are estimated at 3.1 × 10-6, 7.5 × 10-6, 1.7 × 10-5, and 2.8 × 10-4. Exposure to microbial contaminants needs to be evaluated on a single meal basis. For chicken, exposure may range from a single drum stick (38g) to a half broiler (176g) but averages around 80g. For beef between 51 and 85g may be consumed during a single meal. Therefore, methods for monitoring must be able to detect at least 1 CFU/80g. Risks for some pathogenic E. coli are estimated at 1,000 to 10,000 less than Salmonella. Therefore, use of coliforms as indicators needs to be assessed and related to occurrence and survival and regrowth potential of the enteric bacteria of greater public health concern. Because, 20% of the U.S. population may be considered to be in a special population category and at an increased risk of severe outcomes, no more than 20% failure of a standard should be acceptable.
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  • 48
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    Journal of food safety 15 (1995), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ionizing radiation can be an effective step in a HACCP program to kill enteric pathogens associated with meat and poultry products. The populations of most common enteric pathogens such as Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, and Aeromonas hydrophila can be significantly decreased or eliminated by low-dose (〈 3.0 kGy) treatments with ionizing radiation. Only the enteric viruses and the endospores of the genera Clostridium and Bacillus are highly resistant to ionizing radiation, and even these are affected to some degree. Temperature effects must be carefully considered, as reduced irradiation temperatures not only result in fewer adverse changes in the sensorial properties of meat and poultry products, but they also may demand that greater radiation doses be used to inactivate the foodborne pathogen. Equations have been developed that predict the effects of low-dose ionizing radiation treatments on several enteric pathogens associated with meat and poultry. In some cases substrate-specific differences in radiation resistance have been found. Irradiation in combination with vacuum packaging or modified-atmosphere packaging has increased both safety and shelf-life for some types of refrigerated products. High-dose irradiation treatments of enzyme-inactivated meat or poultry products in vacuo at subfreezing temperatures can be used to prepare sterile, shelf-stable products with excellent sensorial properties. Such products have been used extensively by military organizations, NASA during space flights, yachtsman, and hospitals. Contemporary research will be reviewed.
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  • 49
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of modified atmosphere packaging (MAP) (70% CO2/30% N2; 30% CO2/70% N2) on the shelf-life of fresh chicken carcasses stored at 2, 4, 7 and 9C was investigated. The shelf-lives of MAP carcasses (70% CO2/30%N2) stored at 2, 4, 7 and 9C were 25, 21, 12 and 8 days, respectively compared with 7 days for air-packaged ones stored at 4C; the shelf-life of MAP carcasses (30% CO2/70%N2) stored at the same temperatures were 20, 15, 8 and 8 days, respectively. The inhibitory effect of MAP on the growth of Enterobacteriaceae and on the production of spoilage metabolites, such as free fatty acids and extract release volume, was negligible at higher temperatures (7 and 9C) and more pronounced at lower temperatures (2 and 4C), especially at higher concentrations of CO2 (70% CO2/30% N2). The occurrence and growth of organisms such as Escherichia coli, coliforms, Staphylococcus aureus, Salmonella and Campylobacter in carcasses stored at different temperatures were also documented.
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  • 50
    ISSN: 1745-4565
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Polyphosphates are multifunctional ingredients added to many foods, particularly meat products. In addition to their moisture-binding properties, polyphosphates have been reported to inhibit various bacteria. In the current study, four food-grade polyphosphates were evaluated for their effects on the growth of Aeromonas hydrophila K144 in both a model system and a food system. Since polyphosphates can interact with other food factors, effect of these compounds were studied at different NaCl levels and temperatures. The model system was BHI Broth modified by the addition of polyphosphates (sodium pyrophosphate, sodium tripolyphosphate, Hexaphos, or Sodaphos) and NaCl; incubation was aerobic at 5 or 28C. The food system was ground pork with NaCl and polyphosphate added and inoculated with A. hydrophila. Individually, the polyphosphates were relatively noninhibitory in both BHI broth and ground pork, but NaCl and temperature interacted with the polyphosphates in the model system to increase generation and lag times of A. hydrophila. In BHI broth, a combination of 2% of any of the polyphosphates tested and 3.5% NaCl inactivated the bacterium; this inactivation was temperature-dependent. By both a plating system and electron microscopy, the polyphosphate-NaCl combination was shown to injure the bacterium. In the ground pork, the polyphosphate-NaCl combination limited growth of the bacterium during refrigerated storage. These results suggest that polyphosphates could be useful to control the presence of A. hydrophila in certain foods.
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  • 52
    ISSN: 1745-459X
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A battery of sensory tests is proposed to select potential descriptive panelists. This set of tests is flavor specific. Several abilities are examined: odor and taste recognition, odor memory, discrimination and descriptive capacities. A detailed example of such a battery to select a Camembert cheese descriptive panel is given. The objectives of each test are discussed. Stimuli are chosen to be consistent with the sensory properties which can be perceived in cheese. Score distributions demonstrate the discrimination among candidates for each test. Globally, results show the difficulty to find 20 panelists amongst about a hundred with good scores on each test. The panel leader has to choose the final criteria taking into account whether or not some abilities could be improved by training and whether weak capacities (small deficiencies) in some panel members could be compensated for by other subjects.
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  • 53
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    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Taste intensity ratings are higher when many low-intensity stimuli are presented than when many high-intensity stimuli are presented. It is investigated whether similar contextual shifts occur for hedonic judgments.Experiment 1 assessed the perceived pleasantness of a heterogeneous stimulus set containing either a large number of quinine (unpleasant context) or sucrose (pleasant context) solutions. In Experiment 2 subjects made hedonic judgments for a set of sucrose solutions. They were classified pre-experimentally as ‘sweet-likers,’‘neutrals,’ or ‘sweet-dislikers.’ Stimulus frequency distributions were positively or negatively skewed. In Experiment 1, hedonic ratings in the pleasant context were lower than those obtained in the unpleasant context. In Experiment 2, no effect of stimulus context was found.It is argued that contextual shifts in hedonic judgments are found only if the stimuli differ substantially along the hedonic dimension. In addition, the subject groups should be homogeneous with regard to preference rank orders, and should be sufficiently large to allow testing with adequate statistical power.
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  • 54
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    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Although menthol is a common ingredient in pharmaceutical and food products, its sensory properties have not been studied extensively. The objective of this study was to describe and compare the temporal properties of l- and d-menthol. The cooling, burning, and bitterness of two menthol isomers (l-, d-) each at 0.01, 0.02, 0.04, 0.08% (w/v) in aqueous solution were evaluated by 11 trained panelists using time-intensity methodology. The intensity of all three attributes were evaluated continuously from introduction of the sample into the mouth, through expectoration at 10 s, until the termination of the sensation. The l-menthol samples had a greater maximum intensity and longer total duration of cooling and burning sensations than the d-menthol samples. In addition, maximum intensity and total duration of cooling and burn increased with concentration. In contrast, the total duration of the burning sensation was only dependent upon concentration of the l-isomer. Increasing menthol concentration significantly increased maximum intensity and total duration of bitterness for both isomers.
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  • 55
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    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Short-time continuous conching of chocolate offers significant economic advantages over traditional long-time batch methods. High-shear continous mixers can affect conching in a matter of minutes as compared to hours for traditional systems. Milk chocolate conched in two twin-screw, co-rotating, continous mixers operating in series was compared by a variety of sensory methods to chocolate conched by a batch method. A significant difference (P 〈 0.05) in flavor was found between chocolates conched at 60C by the continuous and batch methods, yet there was no preference for either chocolate. Chocolate conched by the batch method (23 h at 60C) had stronger caramel flavor (P 〈 .10) than chocolate conched continuously, but there were no significant differences (P 〈 .10) in sweet, chocolate or milk flavor. In the continuous system, caramel flavor generally increased with conching temperature and residence time; although, at the highest temperature (95C) and the longest residence time (7.5 min) caramel flavor decreased. Increasing the temperature of continuous conching from 70 to 90C produced chocolate significantly (P 〈 .10) more like chocolate conched in a batch system for 21.5 h at 60C.
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  • 56
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    Journal of sensory studies 10 (1995), S. 0 
    ISSN: 1745-459X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper investigates the combined effects of price and product quality (overall liking) on the acceptance of restaurant pizza pies. The results reveal that when a panelist evaluates both liking and purchase intent (with price attached to the purchase intent question), product quality is a significantly stronger determinant of purchase intent than is the stated item price. For instance, a 10% increase in product quality (viz., liking) has the same effect on purchase intent as a 20% reduction in item price. The results are surprising, because for other categories investigated by the same method price is a significantly greater determinant of purchase intent. In terms of predictability, 57% of the variation in purchase intent ratings is accounted for by liking, 24% of the variation is accounted for by price, and the remaining 19% is due to error.
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  • 57
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    Journal of sensory studies 10 (1995), S. 0 
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sipwise and flowise presentation of taste stimuli were compared and better taste discrimination was noted for flowise presentation. Using a model system, discrimination between NaCl solutions and purified water, analysis with atomic absorption spectrophotometry indicated that the superiority of flowise presentation was due to reduction in stimulus variance. Flowise presentation was also superior for discrimination between commercially available natural spring waters.
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  • 58
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    Journal of sensory studies 10 (1995), S. 0 
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A measure of the reliability (T-IR) of time-intensity measurements was developed based on the concept of standard deviation as a measure of panelist variability. The T-IR measure was applied to time-intensity data collected from 10 panelists evaluating the sweetness of 4 model sweetener solutions on horizontal and vertical time-intensity line orientations. T-IR scores showed that the panelists were similarly reliable across the sweeteners and orientations. As well, independent of scale orientation, responses to sweeteners were similarly reliable. The T-IR measure can be used to maintain a high level of performance by monitoring time-intensity panelists. T-IR also provides an objective method of selecting panelists for time-intensity panels.
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  • 59
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    Journal of sensory studies 10 (1995), S. 0 
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Thurstonian modeling predictions were tested and confirmed for 3-AFC and triangle test methods, as well as for different versions of the tetrad test method, using judges who were required to discriminate bitterness in beer. Sequential Sensitivity Analysis predictions were also confirmed for 3-AFC/triangular methods but not for the tetrad method. It is hypothesized that memory effects might have interfered with the later prediction.
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  • 60
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    Journal of sensory studies 10 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Tables of means, over assessors, are often used to summarize the results of sensory profile experiments. These tables are sometimes further summarized by Principal Components Analysis (PCA) to give plots of the samples in the principal sensory dimensions. An alternative procedure is to use Generalized Procrutes Analysis (GPA) on the assessor data to allow for differences in usage of the vocabulary and in the proportion of the scale used. It is shown that these methods give different configurations in the principal sensory dimensions when applied to the data from a study of cheeses (Muir et al. 1995). Using a Jackknife method to calculate the variability of the samples in the principal sensory dimensions, the results from the GPA method are shown to have a higher dimensionality than from the PCA method. Jackknife estimates of variability are used to calculate confidence ellipses to attach to the sensory space maps.
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  • 61
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    Journal of sensory studies 10 (1995), S. 0 
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 62
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 63
    ISSN: 1750-3841
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Precooked longissimus chops and semimembranosus/adductor roasts from pigs (n = 30) given no supplemental vitamin E (CON) or supplemented with 100 mg vitamin E/kg diet (VITE) were evaluated for lipid oxidation, microbial growth, sensory characteristics, cooking/storage losses and reheating losses. Chops and roasts were vacuum packaged, precooked to 60°C and stored at 2°C for 0, 7, 14, 28, or 56 days. Lipid oxidation was lower in VITE chops and roasts than in CON chops and roasts. Off-flavor intensity scores were more acceptable and storage/ cooking losses were lower for VITE roasts than for CON roasts. Supplementation of vitamin E in a swine diet provided added protection against lipid oxidation and precooking pork under vacuum provided a palatable product with a shelf-life of 56 days.
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  • 64
    ISSN: 1750-3841
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hams were processed with standard cure adjuncts under two drying temperatures and analyzed after aging for 7 mo. Dried hams revealed that flavor and texture traits were affected by moisture and nonprotein nitrogen (NPN) content and that NPN was enhanced by greater cathepsin B activity, lower salt levels and higher temperature. Two major defects of dry-cured ham, mushy mouthfeel and surface white film, occurred in about 15% of samples. Most of these hams had abnormal NPN values and were shown to originate from meat with high enzyme activity and low protein content. We concluded that this type of meat is prone to uncontrolled proteolysis and related undesired traits, and to be a potential hazard in dry-cured hams cured with less salt.
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  • 65
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    Journal of food science 60 (1995), S. 0 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A typical channel catfish diet was supplemented with 0, 1.5, and 3% menhaden oil. Fillets were subjected to lipid, fatty acid, and TBA analyses. After 6 mo storage at −18°C, fillets were again analyzed for TBA value and flavor panel profile. Fillets from catfish fed diets with 1.5 and 3% supplemental fish oil had elevated levels of n-3 fatty acids (P〈0.05). TBA number and off-flavor attributes were not affected by treatments. Menhaden oil supplementation up to 3% enhanced the content of n-3 fatty acids in channel catfish fillets without adversely affecting flavor attributes or storage quality.
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  • 66
    ISSN: 1750-3841
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: HPLC methods and modified extraction procedures were used to analyze residues of oxytetracycline (Terramycin, OTC) or sulfadimethoxine (SDM) and ormetoprim (OMP) in channel catfish fed OTC or Romet-30 under controlled conditions. Mean recovery rates in fish muscle were 92.5% for OTC over concentrations of 0.05–1.0 ppm and 86.3% for OMP and 87.9% for SDM over 0.05–5.0 ppm. SDM and OMP were rapidly depleted from large catfish (about 345g) after 5 days feeding with Romet-30 at 50–100 mg/kg body weight. By day 2 post-treatment, no residue was detected. Residues of OTC, SDM and OMP were also detected in fingerling channel catfish (about 20.3g) after 4 and 8 wk of feeding with Romet-30 or OTC at 12.5, 25, or 50 mg/kg body weight, but not after a 3-wk-withdrawal period.
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  • 67
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Renaturation of α1 chains from shark skin collagen (1) was measured by the increase in optical rotation and viscosity. The rotation change of the α1(1) chains became constant after 18 hr, while the viscosity increased further with time. Size exclusion chromatographic patterns of renaturation products of α1 (I) chain gave similar results to that of native collagen. The renaturation products remelted with rising temperature in a similar way to the melting of native collagen as judged from the rotation-temperature curve.
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  • 68
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An activity staining method using SDS-substrate gel was used to determine Pacific whiting proteolytic activity. The most active protease band was resolved at an apparent M, of 39,500 corresponding to the activity of cathepsin L. The activity was more pronounced for ocean-caught Pacific whiting than Puget Sound Pacific whiting or ocean-caught arrowtooth flounder. Gel electrophoresis activity staining of proteolytic enzymes in Pacific whiting was not as sensitive as the TCA-azocasein assay but provided information on the molecular properties of the enzyme and a semi-quantitative estimation of activity.
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  • 69
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Trays containing 100g of mushrooms overwrapped with PVC film were held at 12°C. Two 3 mm holes were made on top of the ovetwrap for ventilation and a TyvekR pouch containing sorbitol was placed at the bottom of the tray before storage. Surface moisture content of mushrooms decreased in the presence of sorbitol. Mushrooms packaged with 10g sorbitol had a constant surface moisture content and those packaged with 15g sorbitol had the best overall color. Principal component analysis of Vis-NIR spectra revealed that surface moisture content affected the scattering of incident light and mushroom color. Lowering of the in-package relative humidity did not affect the maturation rate of mushrooms, but reduced bacterial growth, suggesting that improvement in color was probably due to reduced bacterial activity.
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  • 70
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    Journal of food science 60 (1995), S. 0 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aroma of fresh and hot-air dried bell peppers (Capsicum annuum) was evaluated by sensory and instrumental methods. Hot-air drying decreased levels of the odor compounds (Z)-3-hexenal, 2-heptanone, (Z)-2-hexenal, (E)-2-hexenal, hexanol, (Z)-3-hexanol, (E)-2-hexenol, and linalool, which have green, vegetable-like, fruity, and floral notes, while intensity scores of corresponding sensory aroma attributes also decreased. The aroma of rehydrated dried samples was mainly characterized as savory, rancid/sweaty, sweet/sickly, hay-like, cacao, caramel, and nutty. Drying increased the levels of 2-methylpropanal, 2-and 3-methylbutanal, which have cacao, spicy, and rancid/sweaty odor notes; these volatiles may be correlated with the corresponding aroma attributes in the dried fruits. Principal component analysis demonstrated relationships between instrumental and sensory data for fresh samples, whereas they were more complex for dried fruits.
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  • 71
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ozone exposure was assessed for storage of thornless blackberries which are prone to fungal decay. Blackberries were harvested and stored for 12 days at 2°C in 0.0, 0.1, and 0.3 ppm ozone. Berries were evaluated for fungal decay, anthocyanins, color and peroxidase (POD) activity. Ozone storage suppressed fungal development for 12 days, while 20% of control fruits showed decay. The main mold was Botrytis cinerea. Ozone storage did not cause observable injury or defects. By 12 days, anthocyanin content of juice was similar to initial levels for all treatments. Surface color was better retained in 0.1 and 0.3 ppm-stored berries by 5 days and in 0.3 ppm berries by 12 days, by hue angle values. POD was greater in controls and 0.1 ppm samples, and was lowest in 0.3 ppm fruits by 12 days. Ozone storage resulted in market quality extension.
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  • 72
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two carbonated beverages fortified with 30 mg L-ascorbic acid (AsA) equivalents/250 mL in the form of L-ascorbate 2-monophosphate (AsMP) and stored at 25–35°C retained more vitamin C than those with added AsA or L-ascorbate 2-polyphosphate (AsPP). Breads enriched with ferrous iron and fortified with 560 AsA eq./100g flour in the form of AsPP and AsA retained 40% and 5% vitamin C, respectively, after 6 davs at 25°C. Bran (40%) flakes fortified with 0.19% AsA ea. as AsMP and AsPP gave much improved retention of vitamin C during storage for 7 mo at 25–40°C and 7–11% moisture levels. The sodium salts of AsA, AsMP, and AsPP, when injected into whole eye of round beef at 0.18% AsA eq., inhibited hexanal formation after the meat was roasted and stored for 5 days at 5°C. Those sodium salts did not prevent accumulation of peroxides in peanut paste.
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  • 73
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The susceptibility to heat denaturation of durum wheat β-amylase was studied in aqueous solution at 50 to 65°C. The activation energy of the reaction of denatnration was 439 kJ/mole between 56 and 65°C. Additives were tested to determine their protective effects on enzyme activity (at 65°C) in aqueous solution. Maltose, resulting from degradation of starch by β-amylase, appeared to be the most protective. The β-amylase became more resistant to heat with higher concentrations of maltose, indicating an enzyme-maltose complex which was more stable to heat than the enzyme alone. The same mechanism occurred when durum wheat pasta was processed: maltose produced either during mixing and sheeting or during extrusion protected the enzyme. The degree of protection was proportional to the intensity of mechanical work imparted to the dough.
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  • 74
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sailfish fillets were involved in a 1994 food poisoning outbreak in westem Taiwan. Samples were collected from the victims’residues and from retail and wholesale suppliers. They were analyzed for amines by a gradient elution system of HPLC. Tryptamine, histamine, spermine, trimethylamine, spermidine, and cadaverine were found; other amines were not detectable. The concentration of histamine was higher than 100 mg/100g which was assumed as the toxic level. The allergy-like symptoms of the victims along with the high content of histamine in the sailfish they had eaten were reasonable evidence that the food poisoning was directly associated with histamine intoxication.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oxidation of triolein resulted in the formation of 5 major aldehydes (heptanal, octanal, nonanal, 2-decenal and 2-undecenal). Pre-treatment of the triolein with a twofold molar excess of N2O3 did not qualitatively affect the compounds formed but reduced the amount formed by 63 to 74%. Treatment with tenfold excess of N2O3 resulted in formation of several unknown compounds. However, the ratio of N2O3 to olefinic groups in lipids in cured meats was less than 1:750. These data indicate that nitrite reduced the formation of aldehydes from lipid oxidation and contributed to anti-oxidant activity. However, the amount of nitrite added to meats is probably insufficient for this to be a major anti-oxidant mechanism.
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  • 76
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Acceptability of beef steak tenderness in home and restaurant environments was evaluated by 62 consumers. Steaks (loin, ribeye, or bottom and top round) were consumed by each panelist in their home and in a fine dining restaurant. Each consumer rated steaks for tenderness and overall acceptability in the home (over a 2-mo period) and one steak per week (over a 7-wk period) in the restaurant. Acceptability for steaks consumed at home and in the restaurant ranked between 3 (moderately tough) and 4 (slightly tough) on an 8-point scale. The Warner-Bratzler Shear force transition level for beef tenderness acceptability in the home was between 4.6 and 5.0 kg and in the restaurant between 4.3 and 5.2 kg. The unacceptable level of beef steak tenderness was ≤4.3 kg. Results suggested that consumers were more critical of beef tenderness in the home than in restaurants. Consumer responses may have been influenced by receiving free meals in the home or restaurant, as well as by the dining environment.
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  • 77
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: For propane, conditions were 298K and 308K at the liquid vapor pressure. For supercritical mixtures, conditions were 308K at pressures from 8.9 to 15.7 MPa. The solubility of fat in CO2-propane increased with increasing pressure, up to 0.66wt% at 15.7 MPa. The average solubility of the fat in propane at 298K was 16.7wt% and at 308K it was 21.4wt%. The extracted fat was solid with a melting range of 295–314K. There was no apparent degradation of collagen during extractions. Beef shank fat extraction with propane is feasible and advantageous over use of supercritical CO2-propane.
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  • 78
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pork was cut into small disks, perfused with phosphate buffer, and fiberoptic reflectance was measured as pH was changed under computer control. After myoglobin removal, the effect of pH on paleness was reversible. From pH 7 to pH 5, reflectance increased as pH decreased, and decreased as pH increased (P 〈 0.005). A notable degree (∼40% total reflectance) of light scattering from normal pork may originate from a reversible effect of pH on myofibrils. However, this makes only a minor contribution (∼5% total reflectance) to the very high reflectance of severely pale, soft, exudative pork.
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Boar taint, an unpleasant odor observed in pork from some mature intact male pigs, is attributed primarily to the presence of two compounds in boar fat, androstenone (Sα-androst-16-ene-3-one) and skatole (3-methyl indole). A rapid extraction method for the preparation of fat samples for androstenone/skatole screening assays would lead to more efficient use of boar carcasses for pork production. Supercritical carbon dioxide removed 97 ± 2% of the androstenone (extraction at 40°C; 5 min) and 65 ± 3% of the skatole (extraction at 40°C; 20 min) from 0.5 g of boar backfat. Supercritical CO2 provides the basis for a fairly rapid extraction method to remove compounds associated with boar taint.
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  • 80
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two panels of monoclonal antibodies (Mabs) raised against duck (Barbary) egg white lysozyme or hen egg white lysozyme, were tested in antigen-coated plate (ACP) and double antibody sandwich (DAS) ELISA for cross-reaction with various avian lysozymes. The antibodies to hen lysozyme cross-reacted with goose lysozyme, but most antibodies to duck lysozyme reacted with it. One antibody to duck lysozyme reacted more strongly with goose lysozyme than with the homologous antigen, a heterospecific reaction confirmed by biosensor technology. Many lysozyme epitopes recognized by different antibodies showed considerable resistance to heat denaturation. Such Mabs may be useful for detecting chicken liver adulterants in “foies gras” of goose or duck origin.
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  • 81
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Off-flavor and off-aroma development, which may be catalyzed by lipoxygenase (LPO), are common in frozen stored sweet corn. Lipoxygenase activity in the germ fraction of sweet com (Zea nruys L. cv. Jubilee) was determined and compared with that in the degermed fraction. Lipoxygenase activity/g germ was about three times greater than that of the degermed fraction, Optimized procedures for isolation of lipoxygenase from the germ fraction were developed. Lipoxygenase was isolated by preparation as an acetone powder, extraction with 0.2M TrisHCI, pH 8.0 (4°C), fractionation with 40–60% saturated ammonium sulfate and dialysis. Optimum pH was 6–7 and temperature 50°C for activity of partially purified lipoxygenase. The enzyme appeared stable at pH 5–8 and ∼90% of original activity was inactivated after heating in pH 7 buffer at 70°C for 3 min.
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  • 82
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Descriptive sensory analysis of a homogenate of frozen stored unblan-ched sweet corn indicated that mean overall intensity and most descriptors describing undesirable characteristics were higher than those from blanched corn. To investigate the involvement of corn germ enzymes in off-odor formation, crude enzyme and purified lipoxygenase (LPO) and peroxidase (POD) extracts were prepared and added to homogenates of blanched corn. Addition of the LPO extract increased “painty” and “stale/oxidized” off-odor descriptors and lowered “sweet” and “corn” descriptors. Evidence suggested that sweet corn germ peroxidase is not important in off-odor formation, in which case lipoxygenase may be more appropriate as a blanching indicator.
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  • 83
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Thermal and other physicochemical properties of starch from 42 potato genotypes were studied to find those with unique properties for food use, and to analyze relationships between thermal and other physicochemical properties. Onset and peak transition temperatures and gelatinization enthalpy intercorrelated. Transition temperatures intercorrelated with pasting temperature using a Brabender Visco-amylograph. Gelatinization entbalpy correlated with Brabender pasting temperature and peak paste viscosity, and onset temperature correlated with phosphorus content. Genotype E55–3.5 with highest onset and peak transition temperatures also had highest phosphorus and peak Brabender viscosity. DSC might be useful for rapidly screening samples of 〈1g starch for such. Potato starch DSC characteristics did not correlate with amylose, intrinsic viscosity, or water-binding. For 10 genotypes from successive years, correlations were observed for pasting temperature (r = 0.83), phosphorus content (r = 0.80), and stability ratio (r = 0.66). Direct comparison between samples from consecutive years showed good reproducibility for amylose, but not for phosphorus or pasting.
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  • 84
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Whey protein isolate (WPI) was modified to enhance foaming characteristics by controlled hydrolysis using proteolytic enzymes. Alcalase, acid fungal protease, chymotrypsin, pepsin and trypsin were used to hydrolyze 5% rehydrated WPI. Decree of hydrolvsis was estimated by freezing point depression and terminated at 2.5 to 3% by heating or pH adjustment. Controls were treated under similar conditions but without enzymes. Hydrolysates were separated into permeate and retentate by ultrafiltration and concentrated by reverse osmosis before freeze drying. Foam capacity, stability and surface tension of hydrolysates were measured. Permeate from Alcalase exhibited the best foaming characteristics, comparable to egg white.
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  • 85
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microwave oven heating rates of various oil-in-water emulsions, water-in-oil emulsions, and layered systems were compared. Emulsions heated faster than corresponding layered systems, which heated faster than a calculated weighted average of individual components. Differences were attributed to increased power absorption caused by the large number of interfaces occurring in emulsions, or by a single interface in the layered system, and by resonant absorption of microwave radiation, which is determined by dielectric properties. The types and proportions of emulsions determined the number of interfaces. The dielectric properties and sample size determined in which samples resonant absorption occurred.
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  • 86
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The distribution and chemical form of zinc of several foods prepared from soybean were analyzed. There was a marked variation among soy foods in total zinc, water-soluble zinc, and zinc solubilized on enzymatic digestion. Chromatographic analysis indicated several zinc components were solubilized ion digestion. A significant negative correlation was found for % zinc solubilized after the digestion of soy foods to the original contents of three components, i.e., protein, phosphorus and calcium. Variations among soy foods in chemical form of zinc after digestion should be taken into account on evaluating bioavailability of zinc from soy foods.
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  • 87
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Degradable films from spray-dried (SD) and flash-dried (FD) wheat gluten prepared at various pHs, salt levels, temperatures, and shearing times were compared. Differences were observed in properties of the films related to processing conditions. Films prepared from SD wheat gluten were thicker, and had higher tensile strengths and Young's moduli. SD wheat gluten solutions applied as coating material resulted in Grade A-quality shell eggs maintaining quality for 30 days at room temperature. Additionally, SD wheat gluten coating increased the water stability of starch-based packaging.
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  • 88
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Slabs of agar gel were heated in a microwave oven. Temperatures were measured at various depths into the sample to experimentally determine the internal temperature profile. These were compared to power and temperature profiles predicted from Lambert's law, Maxwell's field equations and a Combined equation. Lambert's law and the Combined equation predicted a much slower heating rate than found experimentally, while Maxwell's field equations gave a much more accurate prediction. Because of the internal standing waves that are created, a small variation in sample thickness could make a large difference in heating rate for thin samples.
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  • 89
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Muscle gels (10% protein) and myofibril gels (8% protein) were prepared at pH 6.0 with 2% NaCl and a heating rate of 0.7°C/min. No difference in gel strength occurred between stretched and cold-shortened muscles, but cooking loss was lower for stretched muscle. Stretched muscle sarcomeres were longer than those of cold-shortened muscle. The myofibril fraction from stretched muscle had higher gel strength, viscosity index, elasticity, and lower cooking loss than that from cold-shortened muscle. These results suggest that the contractile state of the muscle affects protein binding and water binding of the myofibrillar fraction.
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  • 90
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Immunoquantification of myoglobin, L-lactic dehydrogenases (LDH) M4 and H4, albumin, transferrin and immunoglobulin G (IgG) was performed after heat treatment of crude soluble sarcoplasmic protein extract over a range of 54–70°C for 30–780 min. Heat denaturation occurred in the following order: transfenin 〉 IgG 〉 LDH M4 〉 LDH H4 = myoglobin 〉 albumin and could be described by first order reaction kinetics. The very low coefficient of variation (2–4%) of the single radial immunodiffusion technique resulted in a very weak calculated error (± 0.l–0.2°C) on temperature determination. Monitoring the concentration of LDH M4 appears appropriate for determining the minimum heating achieved in long-time, low-temperature cooking and albumin or myoglobin could be monitored for meat cooked at 69°C without holding time.
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  • 91
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    Journal of food science 60 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the addition of antioxidants, spray-dried soy protein isolates (SPI) exhibited increased solubilities by 8% over the control in 0.1M NaCl. Increased solubilities corresponded to a 15% and 8% reduction in protein oxidation as determined by protein carbonyl contents. The greatest differences in solubility between a spray-dried SPI with antioxidant (66% protein solubility) and the control (54% protein solubility), as well as the minimum protein solubility, occurred in 0.2M NaCl. Following the initial solubilization step in SPI processing, amount of extracted proteins was increased by 4.5% over the control with addition of antioxidants. Gel fracturability, hardness and adhesiveness for heat set gels of 12% SPI processed with added antioxidants were increased by 26.3%, 23.5% and 24.6%, respectively.
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  • 92
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    Journal of food science 60 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Four forms of pectinmethylesterase have been partially purified from red grapefruit finisher pulp by chromatography on Heparin-Sepharose CL-6B. Three forms bound to the heparin-sepharose and could be eluted with a sodium chloride gradient. One form (RGPME3) exhibited high thermal stability retaining 66.7% relative activity after 2 min incubation in an 80°C water bath, and 45.2% of its relative activity after 60 set incubation in a 95°C water bath. RGPME3 had a Km of 0.106 mg * mL−1 citrus pectin and a pH optimum between 6 and 7. The most abundant form (RGPMEZ) had a Km of 0.079 mg * mL−1 citrus pectin. Denaturing electrophoresis and gel filtration chromatography suggested molecular weights between 20 and 40 kDa for the various forms.
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  • 93
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    Journal of food science 60 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Anhydrous butteroil or a high-melting milkfat fraction prepared from it were encapsulated in lactose, maltose or sucrose matrices by spray drying to compare ctional characteristics. Both types of milkfat were retained efficiently (〉90%) within the encapsulants at 40% fat level. Retention declined as fat concentration increased from 40 to 60%, regardless of melting temperature of the milk-fat. Encapsulant or type of milkfat did not alter bulk properties. Powders withstood compaction pressures 〈 60% with minimal capsule rupture. Powder functionality may be modified by varying disaccharide choice and milkfat melting properties.
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  • 94
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    Journal of food science 60 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aqueous nonionic (0.3% w/v) and ionic (0.1% and 0.3% w/v) gum systems containing NaCl, or equal weights of NaCl plus KCl, or NaCl plus CaCl, were examined. At equivalent molar concentrations of added ions, 23Na NMR transverse relaxation rates (R2, set−1) showed an increase in average Na+ mobility with the addition of K+ or Ca2+ to ionic gum systems. Correspondingly, salt taste increased with addition of KCl as determined by Decision Boundary modeling of subject identification data. Viscosity did not affect saltiness. Na+ was free to induce salt taste when K+ was bound to the gum. Enhancement of salt taste by KCl is due, in part, to competitive binding of Na+ and K+ in a system.
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  • 95
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some basic components of an indirect competitive enzyme-linked immunosorbent assay (ELISA) were manipulated to reduce sulfathiazole (ST) detection limits. 3.3′.5.5′-Tetramethvlbenzidine (TMB) was more kffeitive than several aliemative chromogens tested for the hetection of horseradish peroxidase activity. Color development was maximized at pH 4.0. Using TMB and a 2M sulfuric acid stopping reagent, an assay was develoued using highly diluted (1:500,000) rabbit serum and low levels (0.0313 μg/mY.), of coating conjugate, with a C value (inflection point of sigmoid standard curve) of 4.4 ppb ST. Urease was unsuitable as a marker for indirect competitive ELISAs.
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  • 96
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    Journal of food science 60 (1995), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some wheats have a 1B/1R translocation and may produce flours that give sticky doughs, even with optimum mixing time and water absorption. Studies showed that the water-soluble fraction caused sticky dough. Dialysis experiments indicated that the responsible substance had low molecular weight. Ionic exchange showed it to be either neutral or negatively charged. Gel filtration chromatography indicated it was both carbohydrate and UV-absorbing. Saponification caused it to lose ability to cause sticky dough. The UV-absorbing material and the carbohydrate had to be linked for the compound to be active. HPLC mass spectrometry indicated the UV-absorbing fraction was ferulic acid and the carbohydrate was a hexose.
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  • 97
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The thermal diffusivity and conductivity of cooked tortillas processed under different conditions were measured at room temperature using a proposed open-photoacoustic cell (OPC) detection technique. Samples were cut from cooked tortillas and exposed to air for different times prior to measurements. Variations in thermal diffusivity inversely correlated with cooking time. Lime appeared to increase the degree of crosslinking, raising thermal diffusivity and decreasing cooking time. By careful process modification a measure of control could be used to improve the heat conduction properties of tortillas.
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  • 98
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Flavonoid, ascorbic acid and total phenolic content and antioxidant activity of “jalapeño” (Veracruz, Mitla, Tam Mild, Jaloro, Sweet Jalapeño), “yellow wax” [(Hungarian Yellow, Long Hot Yellow, Gold Spike (hybrid)], “Chile” (New Mexico-6, Green Chile), “ancho” (San Luis Ancho), and “serrano” (Hidalgo) peppers were investigated at green or yellow stages of maturity. Major pepper flavonoids were quercetin and luteolin which were present in conjugate forms. Total flavonoid content varied from none detectable to 800 mg/kg after hydrolysis. “Chile”, “yellow wax” and “ancho” peppers had greater flavonoid and ascorbic acid contents and antioxidant activities than “jalapeño” peppers. Sep-Pak C18(tm) bound phenolic compounds, including flavonoids, correlated well with antioxidant activity (r2=0.86). Luteolin had highest antioxidant activity followed by capsaicin and quercetin on equimolar basis.
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  • 99
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new peanut line has been developed at the University of Florida with about 80% oleic and 3% linoleic acid. Volatiles and sensory characteristics of roasted normal and high oleic acid peanuts stored at 25°C were compared. Volatiles were analyzed using adsorbent trapping and GCMS, a 20-member trained panel was used for sensory evaluation, and a GC sniffer port was used to evaluate odor characteristics of volatile isolates. Peroxide values were lower for high oleic (HO) peanuts than normal peanuts during storage at 25°C and 40°C. The hexanal content of the peanuts was higher for normal than HO. Peanutty flavor was more stable for HO than normal after 6 wk storage. Painty and cardboard flavors were higher in normal peanuts than HO during storage. Differences for both painty and cardboard flavors were significant after 6 wk storage. Pyrazines were more stable in HO peanuts. Shelf life was estimated from sensory data to be two times longer in HO peanuts.
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  • 100
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    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Predictive microbiology requires a sound basis of observed results, and factors that contribute to death of bacterial cells, before accurate equations can be developed. The effect of chilling and freezing strains of Escherichia coli and salmonella serotypes in nutrient broth, a noninhi-bitory liquid medium, was investigated. The phase of growth, small changes in composition of test medium, and sub-cultures made after primary isolation, influenced survival. Therefore, such influences must be considered when attempting to extrapolate results Erom pure cultures on laboratory media, to predict behavior of similar organisms in foods during chilling and freezing.
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