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  • Conference proceedings  (179)
  • Immunocytochemistry  (123)
  • Springer  (302)
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  • 1990-1994  (302)
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  • Springer  (302)
  • American Chemical Society (ACS)
  • American Geophysical Union
  • American Institute of Physics
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  • 2015-2019
  • 2005-2009
  • 1990-1994  (302)
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  • 1
    ISSN: 1432-1351
    Keywords: Photoreception ; Extraretinal Photoreceptor ; Chromophore ; Opsin ; Reptile ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Since the beginning of this century evidence has accumulated which demonstrates that non-mammalian vertebrates possess photoreceptors situated deep within the brain. While many attempts have been made to localize these sensory cells, studies have either failed or been inconclusive. In this report we have used several experimental approaches to localize the deep brain photoreceptors of the lizard Anolis carolinensis. Using 3 antibodies that bind vertebrate cone opsins, we have immunolabelled cerebrospinal fluid (CSF)-contacting neurons located at the ventricular border within the nucleus ventromedialis of the septum. Western blot analysis indicates that these antibodies recognized a single 40 kD protein in ocular, anterior brain, and pineal extracts. Immunoblots of rodent brain did not show a similar protein band. We have also identified specific retinoids associated with phototransduction (11-cis and all-trans-3,4-didehydroretinaldehyde) within anterior brain extracts. This combined data provides the most detailed analysis of deep brain photoreceptors in any vertebrate. Consequently, we feel Anolis provides an excellent model to study this unexplored sensory system of the vertebrates.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1351
    Keywords: Egg laying hormone ; Aplysia ; Binding protein ; Immunocytochemistry ; Reproductive system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A protein solubilized from a membrane preparation of the gonad of Aplysia californica has been isolated by affinity chromatography, using bag cell egg-laying hormone (ELH) as the bound ligand, and partially purified and characterized by gel electrophoresis. The protein has an apparent molecular weight of 52 kDa and consists of two disulfide-linked subunits of about 30 kDa each. The protein is glycosylated and has an acidic pI. Approximately 10–15 μg of this protein can be isolated from a single ovotestis, representing less than 1% of the total protein in the gonad; but the protein could not be detected in buccal mass or body wall, tissues which do not have apparent response to ELH. Antibodies generated against this ELH-binding protein (ELHBP) were used to localize sites in the ovotestis which might contain this molecule and thus represent targets for egg-laying hormone. Immunocytochemical results indicate that the oocytes are a rich source of this protein, since their cytoplasm was the only detectable site of immunoreactivity. Whether this binding protein represents an egg-laying hormone receptor is uncertain, but its prevalence in oocytes suggests that ELH plays a signaling role on these gametes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 125 (1994), S. 107-117 
    ISSN: 1573-0832
    Keywords: Aflatoxin B1 ; Immunocytochemistry ; Regeneration ; Tissue culture ; Tobacco plantlets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of aflatoxin B1, (0.5–25 µg ml−1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 µg ml−1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 µg ml−1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1715-1721 
    ISSN: 0392-6737
    Keywords: Fluctuations (noise, chaos, nonequilibrium superconductivity, localization, etc.) ; Flux pinning ; flux motion ; fluxon-defect interactions ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary High-quality single-phase thin platelets of BSCCO-2223 have been produced by a novel method on thermal-gradient-enhanced KCl flux which allows the growth of the 2223-phase in an extremely short time interval compared to those reported in the literature. In-plane electrical resistivity measurements in zero applied magnetic field have been performed. Experimental data have been analysed and interpreted within the Aslamazov-Larkin theory of amplitude fluctuations and the Ginzburg-Landau Coulomb gas model of phase fluctuations. These theories seem to fit in a very reliable way the experimentalR(T) curves.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1815-1820 
    ISSN: 0392-6737
    Keywords: Insulator-superconductor transitions ; unconventional mechanisms of high-T c superconductivity ; Other topics in superconductivity ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The temperature dependence of local Cu site conformations in single-domain crystals of Bi2Sr2CaCu2O8+y (Bi-2212) and La1.85Sr0.15CuO4 (La-214) has been determined by EXAFS (Extended X-ray Absorption Fine Structure), a fast (10−15 s) and local probe. Two sets of Cu site conformations characterized by the Cu−O(apical) bond distances and tilting of copper square plane have been found atT〈T*∼1.5T c . The difference between the long and short Cu−O(apical) distance appeared to be 0.17 Å and 0.12 Å atT〈T* in Bi-2212 and La-214 systems, respectively. The polarons are associated with the short Cu−O(apical) bond distance. The ratio between the number of short and the total number of Cu−O(apical) bonds (N short/N tot∼0.42 in both systems) is a measure of the relative area of distorted and undistorted domains. By using this result we have been able to show the polaronic nature of the one-dimensional structural anharmonic modulation of the CuO2 plane in Bi-2212 where the polarons have dimensionW=(11±1) Å atT〈T*≈1.4T c and are condensed into a one-dimensional generalized Wigner charge density wave (CDW). It appears that superconductivity is stabilized at thigh temperature by resonant quantum confinement in a superlattice of stripe widthL wherek Fx ∼k Fy ∼π/L.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1843-1849 
    ISSN: 0392-6737
    Keywords: Fluctuations (noise, chaos, nonequilibrium superconductivity, localization, etc.) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary This work is focused on how oxygen stoichiometry and distribution affects the pretransitional region in YBa2Cu3O7−δ pellets prepared by different thermal treatments starting from the same batch with oxygen content 6.94. We find that slightly deoxygenated samples, which show higherT c and narrower transition than the starting material, also exhibit a more pronounced rounding of the resistivity extending towards higher temperatures. We analyse the presence and the extension of regions with different power law behaviour of excess conductivityvs. temperature.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1871-1876 
    ISSN: 0392-6737
    Keywords: Magnetization curves ; Meissner effect ; penetration depth ; Critical currents ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary BSCCO (2223) phase superconductor, prepared by the traditional solid-state reaction route, was densified by hot pressing, yielding high-density and textured-bulk materials. A correlation between processing parameters and inter-, intra-granular properties of the sample was performed through the evaluation of microstructural features and the study of electrical resistivity and magnetization behaviour as a function of different temperature and magnetic field.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1925-1932 
    ISSN: 0392-6737
    Keywords: Flux pinning ; flux motion ; fluxon-defect interactions ; Critical currents ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary The results of low-temperature magnetic-relaxation experiments in TlBaCaCuO superconductors (2212 and 2223 phase) are reported. They give evidence of a crossover from a thermally activated regime of flux motion to a nonthermally activated one. The magnetic-relaxation rate is found to become temperature independent below ≅7K and ≅3K, for the 2212 and 2223 phase, respectively. Its value compares well with that predicted by the theories of flux motion by quantum tunnelling.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1917-1924 
    ISSN: 0392-6737
    Keywords: Flux pinning ; flux motion ; fluxon-defect interactions ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Measurements of fundamental and higher harmonics of a.c. susceptibility on polycrystalline high-T c superconductors were carried out using a new method, involving a 2-channel dynamic analyser, allowing 20 harmonics in phase and out of phase to be measured at the same time. The critical-state model, used for describing the higher-harmonics dependence on temperature at fixed d.c. and a.c. magnetic fields, did not explain the experimental results. A frequency analysis indeed suggested that an effect of the fluxon dynamics takes place. Simple considerations lead to the conclusion that really Thermally Activated Flux Flow (TAFF), Flux Creep (FC) and Flux Flow (FF) would have a big role in determining the higher harmonics of a.c. susceptibility. Measurements on low-T c superconductors showed the occurrence of the same effect.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 16 (1994), S. 1933-1939 
    ISSN: 0392-6737
    Keywords: Flux pinning, flux creep, and flux-line lattice dynamics ; Critical currents ; Material effects onT c, κ, critical currents (including impurities, ion implantation, etc.) ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Bulk slabs of sintered YBCO were irradiated with monoenergetic protons at different energies (3.5 and 6.5 MeV) and different fluences, ranging from 1.8·1016 to 8.6·1016 p/cm2. The effects on intergrain critical currents of protons crossing the sample and the effects of protons implanting in the specimen, has been studied by means of a.c. susceptibility characterizations. D.c. hysteresis cycles were measured in low fields in order to investigate the modifications in the intergrain critical current density due to proton implantation. The main result is the evidence that the intergrainJ c response to the applied field can be modulated by means of the proton irradiation. Resistive measurements were also performed in order to investigate the complex transport phenomena in polycrystalline materials. Irradiation-induced effects on both superconducting- and normal-state resistivity are studied. The addition of extrinsic defects can lead to enhanced transport properties, emerging especially when a magnetic field is applied.
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