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  • 1
    Digitale Medien
    Digitale Medien
    Reachability of locational Nash equilibria (1998)
    Springer
    OR spectrum 20 (1998), S. 101-107 
    Details
    ISSN: 1436-6304
    Schlagwort(e): Competitive location model ; Nash equilibria ; stability ; reachability ; Wettbewerbsmodelle in der Standorttheorie ; Nash Gleichgewicht ; Stabilität ; Erreichbarkeit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik , Wirtschaftswissenschaften
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung In der Arbeit werden die Standorte von Duopolisten in einem Baum untersucht. Unter der Annahme festgesetzter Preise werden notwendige und hinreichende Bedingungen für Nash Gleichgewichte für Standorte auf Bäumen hergeleitet. Unter Verwendung dieser Bedingungen wird dann gezeigt, daß — angenommen Nash Gleichgewichte existieren — diese in einem wiederholt angewandten sequentiellen Standortfindungsprozeß, in dem beide Duopolisten als Zielfunktion kurzfristige Gewinnmaximierung haben, auch erreicht werden.
    Notizen: Abstract This paper examines the location of duopolists on a tree. Given parametric prices, we first delineate necessary and sufficient conditions for locational Nash equilibria on trees. Given these conditions, we then show that Nash equilibria, provided they exist, can be reached in a repeated sequential relocation process in which both facilities follow short-term profit maximization objectives.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01539861
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  • 2
    Digitale Medien
    Digitale Medien
    Reachability of locational Nash equilibria (1998)
    Springer
    OR spectrum 20 (1998), S. 101-107 
    Details
    ISSN: 1436-6304
    Schlagwort(e): Key words: Competitive location model ; Nash equilibria ; stability ; reachability ; Schlüsselwörter: Wettbewerbsmodelle in der Standorttheorie ; Nash Gleichgewicht ; Stabilität ; Erreichbarkeit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik , Wirtschaftswissenschaften
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung. In der Arbeit werden die Standorte von Duopolisten in einem Baum untersucht. Unter der Annahme festgesetzter Preise werden notwendige und hinreichende Bedingungen für Nash Gleichgewichte für Standorte auf Bäumen hergeleitet. Unter Verwendung dieser Bedingungen wird dann gezeigt, daß– angenommen Nash Gleichgewichte existieren – diese in einem wiederholt angewandten sequentiellen Standortfindungsprozeß, in dem beide Duopolisten als Zielfunktion kurzfristige Gewinnmaximierung haben, auch erreicht werden. “Equilibrium is a place in heaven, but how do we get there from here?”
    Notizen: Abstract. This paper examines the location of duopolists on a tree. Given parametric prices, we first delineate necessary and sufficient conditions for locational Nash equilibria on trees. Given these conditions, we then show that Nash equilibria, provided they exist, can be reached in a repeated sequential relocation process in which both facilities follow short-term profit maximization objectives.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002910050058
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  • 3
    Digitale Medien
    Digitale Medien
    Money demand stability: Evidence from Spain (1998)
    Springer
    Empirical economics 23 (1998), S. 387-400 
    Details
    ISSN: 1435-8921
    Schlagwort(e): Key words: Money demand ; cointegration ; stability ; regime shift ; JEL classification: E41 ; C22
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Wirtschaftswissenschaften
    Notizen: Abstract. Using several tests for structural stability in regressions with I(1) variables and for the existence of cointegration in models with regime shifts, the empirical evidence on the existence of a structural break in the Spanish long-run demand for broad money (ALP2) is analysed.  The results indicate that shifts affecting the demand for ALP2 in recent years have substantially altered its long-run properties. As to the cause of this structural break, emphasis is placed on the role played by the increasing openness of the Spanish financial system to international markets as obstacles to free capital movements have progressively disappeared.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001810050027
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  • 4
    Digitale Medien
    Digitale Medien
    Discrete stability of the Differential System Method evaluated with geostatistical techniques (1998)
    Springer
    Stochastic environmental research and risk assessment 12 (1998), S. 191-204 
    Details
    ISSN: 1436-3259
    Schlagwort(e): Keywords: groundwater flow ; inverse problems ; stability ; geostatistical interpolation ; kriging.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Architektur, Bauingenieurwesen, Vermessung , Energietechnik , Geographie , Geologie und Paläontologie
    Notizen: Abstract The Differential System Method (DSM) permits identification of the physical parameters of finite-difference groundwater flow models in a confined aquifer when piezometric head and source terms are known at each point of the finite-difference lattice for at least two independent flow situations for which the hydraulic gradients are not parallel. Since piezometric head data are usually few and sparse, interpolation of the measured data onto a regular grid can be performed with geostatistical techniques. We apply kriging to the sparse data of a synthetic aquifer to evaluate the stability of the DSM with respect to uncorrelated measurement errors and interpolation errors. The numerical results show that the DSM is stable.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004770050017
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  • 5
    Digitale Medien
    Digitale Medien
    Characterization of stable matchings as extreme points of a polytope (1992)
    Springer
    Mathematical programming 54 (1992), S. 57-67 
    Details
    ISSN: 1436-4646
    Schlagwort(e): Matchings ; stability ; extreme points ; polytope
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik , Mathematik
    Notizen: Abstract The purpose of this paper is to extend a modified version of a recent result of Vande Vate (1989) which characterizes stable matchings as the extreme points of a certain polytope. Our proofs are simpler and more transparent than those of Vande Vate.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01586041
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  • 6
    Digitale Medien
    Digitale Medien
    A Two-Patch Model of Gambian Sleeping Sickness: Application to Vector Control Strategies in a Village and Plantations (1998)
    Springer
    Acta biotheoretica 46 (1998), S. 207-222 
    Details
    ISSN: 1572-8358
    Schlagwort(e): Epidemiology ; sleeping sickness ; vector controls ; differential equations ; spacialization ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A compartmental model is described for the spread of Gambian sleeping sickness in a spatially heterogeneous environment in which vector and human populations migrate between two "patches": the village and the plantations. The number of equilibrium points depends on two "summary parameters": gr the proportion removed among human infectives, and R0, the basic reproduction number. The origin is stable for R0 〈1 and unstable for R0 〉1. Control strategies are assessed by studying the mix of vector control between the two patches that bring R0 below 1. The results demonstrate the importance of vector control in the plantations. For example if 20 percent of flies are in the village and the blood meal rate in the village is 10 percent, then a 20 percent added vector mortality in the village must be combined with a 9 percent added mortality in the plantations in order to bring R0 below 1. The results are quite insentive to the blood meal rate in the village. Optimal strategies (that minimize the total number of flies trapped in both patches) are briefly discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1001776926167
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  • 7
    Digitale Medien
    Digitale Medien
    Phthalocyanine-containing polymers (1992)
    Springer
    Journal of inorganic and organometallic polymers and materials 2 (1992), S. 79-85 
    Details
    ISSN: 1572-8870
    Schlagwort(e): Phthalocyanines ; polymers ; siloxanes ; conductivity ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The author's work on the incorporation of phthalocyanines into inorganic polymers is reviewed. The synthesis of poly(siloxane phthalocyanines) and the synthesis and characterization of fluoro(phthalocyanine) Group III compounds and their conducting derivatives are described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00696537
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  • 8
    Digitale Medien
    Digitale Medien
    Analytical relationships between the ionization of 1,3-dicarbonylic ligands and stability of their Cr(III), Ni(II), Co(II) and Cu(II) 1∶1 chelates (1992)
    Springer
    Journal of solution chemistry 21 (1992), S. 1145-1152 
    Details
    ISSN: 1572-8927
    Schlagwort(e): Chromium(III) ; β-diketones ; β-diketonates ; chelation ; complexation ; correlation ; stability ; equilibrium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract It has been demonstrated that the logarithm of the stability constant of some monochelated chromium(III) compounds, with structurally similar 1,3-dicarbonylic species, is linearly related to the negative logarithm of the acid ionization constant of the ligand. Graphical and analytical correlations which could be useful in predicting equilibrium constants of chromium(III)-β-diketonates, as well as other first-row transition metal derivatives, have been developed. A quantitative evaluation of the complexes stability has been carried out, providing information about the effects of ligand substituents on the equilibrium constants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00651860
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  • 9
    Digitale Medien
    Digitale Medien
    Stability and Instability of Fourth-Order Solitary Waves (1998)
    Springer
    Journal of dynamics and differential equations 10 (1998), S. 151-188 
    Details
    ISSN: 1572-9222
    Schlagwort(e): Fourth-order solitary waves ; stability ; instability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract We study ground-state traveling wave solutions of a fourth-order wave equation. We find conditions on the speed of the waves which imply stability and instability of the solitary waves. The analysis depends on the variational characterization of the ground states rather than information about the linearized operator.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1022644629950
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  • 10
    Digitale Medien
    Digitale Medien
    Interval stability and interval covering property in finite posets (1992)
    Springer
    Order 9 (1992), S. 163-175 
    Details
    ISSN: 1572-9273
    Schlagwort(e): Primary 06A07 ; secondary 05C70 ; Partial order ; interval ; stability ; covering ; Sperner property ; symmetric chains ; NP-completeness
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract Given a finite ranked posetP, let α(P) be the maximum size of a subset ofP such that no two elements of it belong simultaneously to some interval ofP and let ϱ(P) be the minimum number of intervals covering all elements ofP. We say thatP has the strong interval stability property (resp. the strong interval covering property) if for each subposetP′ induced by consecutive levels ofP, i.e.,P′=P (l)∪...∪P (u), one has α(P′)=max{|P (l)|, |P (u)|} (resp. ϱ(P′)=max{|P (l)|, |P (u)|}). We prove these properties for several classes of posets and discuss some general facts concerning the numbers α(P) and ϱ(P), e.g., NP-completeness and min-max relations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00814408
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  • 11
    Digitale Medien
    Digitale Medien
    Some Results on Stability of Retarded Functional Differential Equations Using Dichotomic Map Techniques (1998)
    Springer
    Positivity 2 (1998), S. 229-238 
    Details
    ISSN: 1572-9281
    Schlagwort(e): asymptotic stability ; dichotomic maps ; retarded functional differential equation ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract This paper deals with the study of the stability of nonautonomous retarded functional differential equations using the theory of dichotomic maps. After some preliminaries, we prove the theorems on simple and asymptotic stability. Some examples are given to illustrate the application of the method. Main results about asymptotic stability of the equation $$x'(t) = - b(t)x(t - r)$$ and of itsnonlinear generalization $$x'(t) = b(t)f(x(t - r))$$ are established.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009742807025
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  • 12
    Digitale Medien
    Digitale Medien
    Stationary waiting time derivatives (1992)
    Springer
    Queueing systems 12 (1992), S. 369-389 
    Details
    ISSN: 1572-9443
    Schlagwort(e): Perturbation analysis ; stability ; stochastic difference equations ; simulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract We investigate the stability of waiting-time derivatives when inputs to a queueing system-service times and interarrival times-depend on a parameter. We give conditions under which the sequence of waiting-time derivatives admits a stationary distribution, and under which the derivatives converge to the stationary regime from all initial conditions. Further hypotheses ensure that the expectation of a stationary waiting-time derivative is, in fact, the derivative of the expected stationary waiting time. This validates the use of simulation-based infinitesimal perturbation analysis estimates with a variety of queueing processes. We examine waiting-time sequences satisfying recursive equations. Our basic assumption is that the input and its derivatives are stationary and ergodic. Under monotonicity conditions, the method of Loynes establishes the convergence of the derivatives. Even without such conditions, the derivatives obey a linear difference equation with random coefficients, and we exploit this fact to find stability conditions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01158809
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  • 13
    Digitale Medien
    Digitale Medien
    On the stability of a partially accessible multi‐station queue with state‐dependent routing (1998)
    Springer
    Queueing systems 29 (1998), S. 55-73 
    Details
    ISSN: 1572-9443
    Schlagwort(e): multi‐server queue ; customer class ; state‐dependent routing ; stability ; Markov chain ; fluid limit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract We consider a multi‐station queue with a multi‐class input process when any station is available for the service of only some (not all) customer classes. Upon arrival, any customer may choose one of its accessible stations according to some state‐dependent policy. We obtain simple stability criteria for this model in two particular cases when service rates are either station‐ or class‐independent. Then, we study a two‐station queue under general assumptions on service rates. Our proofs are based on the fluid approximation approach.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1019175812444
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  • 14
    Digitale Medien
    Digitale Medien
    On the guaranteed throughput and efficiency of closed re-entrant lines (1998)
    Springer
    Queueing systems 28 (1998), S. 33-54 
    Details
    ISSN: 1572-9443
    Schlagwort(e): queueing networks ; throughput ; closed networks ; efficiency ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract A closed network is said to be “guaranteed efficient” if the throughput converges under all non-idling policies to the capacity of the bottlenecks in the network, as the number of trapped customers increases to infinity. We obtain a necessary condition for guaranteed efficiency of closed re-entrant lines. For balanced two-station systems, this necessary condition is almost sufficient, differing from it only by the strictness of an inequality. This near characterization is obtained by studying a special type of virtual station called “alternating visit virtual station”. These special virtual stations allow us to relate the necessary condition to certain indices arising in heavy traffic studies using a Brownian network approximation, as well as to certain policies proposed as being extremal with respect to the asymptotic loss in the throughput. Using the near characterization of guaranteed efficiency we also answer the often pondered question of whether an open network or its closed counterpart has greater throughput - the answer is that neither can assure a greater guaranteed throughput.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1019199022922
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  • 15
    Digitale Medien
    Digitale Medien
    Stability of token passing rings (1992)
    Springer
    Queueing systems 11 (1992), S. 7-33 
    Details
    ISSN: 1572-9443
    Schlagwort(e): Token passing rings ; stability ; substability ; ergodicity ; Markov chains ; Loynes' scheme ; stochastically dominant ; Little's formula ; regenerative processes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract A sufficient stability condition for the standard token passing ring has been “known” since the seminal paper by Kuehn in 1979. However, this condition was derived without formal proof, and the proof seems to be of considerable interest to the research community. In fact, Watson observed that in the performance evaluation of token passing rings, “it is convenient to derive stability conditions ... (without proof)”. Our intention is to fill this gap, and to provide a formal proof of thesufficient and necessary stability condition for the token passing ring. In this paper, we present the case when the arrival process to each queue is Poisson but service times and switchover times are generally distributed. We consider in depth a gatedl-limited (l≤ ∞) service discipline for each station. We also indicate that the basic steps of our technique can be used to study the stability of some other multiqueue systems.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01159285
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  • 16
    Digitale Medien
    Digitale Medien
    Stability and performance analysis of rate-based feedback flow controlled ATM networks (1998)
    Springer
    Queueing systems 29 (1998), S. 129-159 
    Details
    ISSN: 1572-9443
    Schlagwort(e): rate-based feedback control ; ATM networks ; stability ; optimal algorithms
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract Motivated by ABR class of service in ATM networks, we study a continuous time queueing system with a feedback control of the arrival rate of some of the sources. The feedback about the queue length or the total workload is provided at regular intervals (variations on it, especially the traffic management specification TM 4.0, are also considered). The propagation delays can be nonnegligible. For a general class of feedback algorithms, we obtain the stability of the system in the presence of one or more bottleneck nodes in the virtual circuit. Our system is general enough that it can be useful to study feedback control in other network protocols. We also obtain rates of convergence to the stationary distributions and finiteness of moments. For the single botterneck case, we provide algorithms to compute the stationary distributions and the moments of the sojourn times in different sets of states. We also show analytically (by showing continuity of stationary distributions and moments) that for small propagation delays, we can provide feedback algorithms which have higher mean throughput, lower probability of overflow and lower delay jitter than any open loop policy. Finally these results are supplemented by some computational results.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1019127929282
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  • 17
    Digitale Medien
    Digitale Medien
    Stable steady state of some population models (1992)
    Springer
    Journal of dynamics and differential equations 4 (1992), S. 161-190 
    Details
    ISSN: 1572-9222
    Schlagwort(e): Delay differential equations ; equilibrium ; stability ; limiting equations ; population dynamics ; 34K20 ; 34K25 ; 92A15
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract Applying an analytical method and several limiting equations arguments, some sufficient conditions are provided for the existence of a unique positive equilibriumK for the delay differential equationx=−γx+D(x t ), which is the general form of many population models. The results are concerned with the global attractivity, uniform stability, and uniform asymptotic stability ofK. Application of the results to some known population models, which shows the effectiveness of the methods applied here, is also presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01048159
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  • 18
    Digitale Medien
    Digitale Medien
    Stability estimates in inverse scattering (1992)
    Springer
    Acta applicandae mathematicae 28 (1992), S. 1-42 
    Details
    ISSN: 1572-9036
    Schlagwort(e): 35R30 ; Inverse scattering ; stability ; noisy data
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract An algorithm is given for calculating the solution to the 3D inverse scattering problem with noisy discrete fixed energy data. The error estimates for the calculated solution are derived. The methods developed are of a general nature and can be used in many applications: in nondestructive evaluation and remote sensing, in geophysical exploration, medical diagnostics, and technology.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00047029
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  • 19
    Digitale Medien
    Digitale Medien
    Multiwavelet approximation methods for pseudodifferential equations on curves. Stability and convergence analysis (1998)
    Springer
    Advances in computational mathematics 9 (1998), S. 145-171 
    Details
    ISSN: 1572-9044
    Schlagwort(e): periodic pseudodifferential equations ; multiwavelets ; splines with multiple knots ; generalized Galerkin–Petrov schemes ; boundary element methods ; error analysis ; stability ; Strang–Fix condition ; 65J10 ; 65N30 ; 65N35 ; 65R20 ; 47G30 ; 45P05 ; 41A25 ; 41A30 ; 41A15
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract We develop a stability and convergence analysis of Galerkin–Petrov schemes based on a general setting of multiresolution generated by several refinable functions for the numerical solution of pseudodifferential equations on smooth closed curves. Particular realizations of such a multiresolution analysis are trial spaces generated by biorthogonal wavelets or by splines with multiple knots. The main result presents necessary and sufficient conditions for the stability of the numerical method in terms of the principal symbol of the pseudodifferential operator and the Fourier transforms of the generating multiscaling functions as well as of the test functionals. Moreover, optimal convergence rates for the approximate solutions in a range of Sobolev spaces are established.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018977120831
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  • 20
    Digitale Medien
    Digitale Medien
    A new interpolation procedure for adapting Runge-Kutta methods to delay differential equations (1992)
    Springer
    BIT 32 (1992), S. 634-649 
    Details
    ISSN: 1572-9125
    Schlagwort(e): AMS(MOS): 65L20 ; CR: 5.17 ; delay differential equations ; numerical solution ; Runge-Kutta methods ; interpolation procedures ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract This paper deals with adapting Runge-Kutta methods to differential equations with a lagging argument. A new interpolation procedure is introduced which leads to numerical processes that satisfy an important asymptotic stability condition related to the class of testproblemsU′(t)=λU(t)+μU(t−τ) with λ, μ ε C, Re(λ)〈−|μ|, and τ〉0. Ifc i denotes theith abscissa of a given Runge-Kutta method, then in thenth stept n−1→t n :=t n−1+h of the numerical process our interpolation procedure computes an approximation toU(t n−1+c i h−τ) from approximations that have already been generated by the process at pointst j−1+c i h(j=1,2,3,...). For two of these new processes and a standard process we shall consider the convergence behaviour in an actual application to a given, stiff problem.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01994847
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  • 21
    Digitale Medien
    Digitale Medien
    On a class of variational equations transformable to the Gauss hypergeometric equation (1992)
    Springer
    Celestial mechanics and dynamical astronomy 53 (1992), S. 145-150 
    Details
    ISSN: 1572-9478
    Schlagwort(e): Monodromy matrix ; Gauss hypergeometric equation ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A new class of linear ordinary differential equations with periodic coefficients is found which can be transformed to the Gauss hypergeometric equation, and therefore the monodromy matrices are computable explicitly. These equations appear as the variational equations around a straight-line solution in Hamiltonian systems of the form H = T(p) + V(q), where T(p) and V(q) are homogeneous functions of p and q, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00049462
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  • 22
    Digitale Medien
    Digitale Medien
    Effects of small external forces on the planar oscillation of a cable connected satellites system (1992)
    Springer
    Celestial mechanics and dynamical astronomy 53 (1992), S. 219-226 
    Details
    ISSN: 1572-9478
    Schlagwort(e): Artificial satellite ; dissipative forces ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The effects of small external dissipative and disturbing forces on the non-linear planar oscillation of a cable connected satellites system in the central gravitational field of earth have been studied. Typical non-linear oscillation's phenomena arizing from the aforesaid external forces are shown to take place. The presence of these forces enables the application of asymptotic methods of the theory of non-linear oscillations due to Bogoliubov and Mitropolsky to the equation characterizing the non-linear oscillation of the system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00052610
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  • 23
    Digitale Medien
    Digitale Medien
    Nonlinear Stability of the Lagrangian Libration Points in the Chermnykh Problem (1998)
    Springer
    Celestial mechanics and dynamical astronomy 70 (1998), S. 41-58 
    Details
    ISSN: 1572-9478
    Schlagwort(e): three-body problem ; libration points ; stability ; normal forms.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract In this paper we consider the problem of motion of an infinitesimal point mass in the gravity field of an uniformly rotating dumb-bell. The aim of our study is to investigate Liapunov stability of Lagrangian libration points of this problem. We analyze the stability of libration points in the whole range of parameters ω, μ of the problem. In particular, we consider all resonance cases when the order of resonance is not greater than five.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008250207046
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  • 24
    Digitale Medien
    Digitale Medien
    On a spherical integral transformation and sections of star bodies (1998)
    Springer
    Monatshefte für Mathematik 126 (1998), S. 117-124 
    Details
    ISSN: 1436-5081
    Schlagwort(e): 52A20 ; 52A22 ; star bodies ; spherical integral transformations ; convex bodies ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract LetK be ad-dimensional star body (with respect to the origino). It is known that the (d−1)-dimensional volume of the intersections ofK with the hyperplanes througho does not uniquely determineK. Uniqueness can only be achieved under additional assumptions, such as central symmetry. Here it is pointed out that if one uses, instead of intersections by hyperplanes, intersections by half-planes that containo on the boundary, then, without any additional assumptions, the volume of these intersections determinesK uniquely. This assertion, and more general results of this kind, together with stability estimates, are obtained from uniqueness results and estimates concerning a particular spherical integral transformation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01473582
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  • 25
    Digitale Medien
    Digitale Medien
    Ethene hydroformylation on Co/SiO2 catalysts (1998)
    Springer
    Catalysis letters 53 (1998), S. 97-101 
    Details
    ISSN: 1572-879X
    Schlagwort(e): ethene hydroformylation ; heterogeneous catalysts ; cobalt catalysts ; gas‐phase deposition ; dispersion ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The results from ethene hydroformylation at 173°C showed that a Co(acac)3/SiO2 catalyst prepared from Co(acac)3 precursor by gas‐phase deposition was three times as active as a catalyst prepared by impregnation from cobalt nitrate, but oxo‐selectivities were similar. The high propanal selectivities on the Co(acac)3/SiO2 seem to be related to the presence of highly dispersed active sites favouring CO insertion. As dispersion is decreased from 23 to 8% due to increasing metal content (from 5 to 16 wt%), oxo‐selectivity decreased from 39 to 25%. The activity of Co(acac)3/SiO2 remained unchanged during 68 h on stream. The gas‐phase deposition technique described here is a promising method for the preparation of active, selective and stable heterogeneous hydroformylation catalysts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1019053805618
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  • 26
    Digitale Medien
    Digitale Medien
    Preparation of model catalysts by laser interference nanolithography followed by metal cluster deposition (1998)
    Springer
    Catalysis letters 56 (1998), S. 1-6 
    Details
    ISSN: 1572-879X
    Schlagwort(e): nanolithography ; model catalyst ; palladium ; copper ; stability ; spin-coating ; SEM ; AFM ; XPS
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Metal clusters arranged on nanostructured oxidized silicon wafers are presented as new model catalyst systems. A photoresist layer spun on top of a wafer was patterned by laser interference exposure. The grid obtained after removing the exposed parts of the resist is used as an etching mask. Hollows with diameters of 300 nm and depths between 50 and 60 nm were etched into the oxide layer using wet chemical methods. Two methods were applied to deposit metal clusters (Pd or Cu) in a defined way within the hollows. The particles ranged from 10 to 50 nm in height and from 80 to 200 nm in diameter. The model catalyst systems were characterized by atomic force microscopy and X-ray photoelectron spectroscopy. The method presented here allows us to produce 4 inch wafers that are covered completely by nanometer-sized structures in a reasonable period of time.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1019080222823
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  • 27
    Digitale Medien
    Digitale Medien
    Nuclear calcium: a key regulator of gene expression (1998)
    Springer
    BioMetals 11 (1998), S. 345-358 
    Details
    ISSN: 1572-8773
    Schlagwort(e): calcium ; CREB ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Through the evolution of multicellular organisms, calcium has emerged as the preferred ion for intracel-lular signalling. It now occupies a pivotal role in many cell types and nowhere is it more important than in neurons, where it mediates both the relaying and long-term storage of information. The latter is a process that enables learning and memory to be formed and requires the activation of gene expression by calcium signals. Evidence from a number of diverse organisms shows that transcription mediated by the transcrip-tion factor CREB is critical for learning and memory. Here we review the features of CREB activation by calcium signals in mammalian cells. In contrast to other transcription factors, its regulation is dependent on an elevation of nuclear calcium concentration, potentially placing this spatially distinct pool of calcium as an important mediator of information storage.© Kluwer Academic Publishers
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009257909785
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  • 28
    Digitale Medien
    Digitale Medien
    Multiplex Titration RT-PCR: Rapid Determination of Gene Expression Patterns for a Large Number of Genes (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 323-339 
    Details
    ISSN: 1572-9818
    Schlagwort(e): Aux/IAA genes ; gene expression ; gene families ; RT-PCR ; tomato
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have developed an improved method for determination of gene expression levels with RT-PCR. The procedure is rapid and does not require extensive optimization or densitometric analysis. Since the detection of individual transcripts is PCR-based, small amounts of tissue samples are sufficient for the analysis of expression patterns in large gene families. Using this method, we were able to rapidly screen nine members of the Aux/IAA family of auxin- responsive genes and identify those genes which vary in message abundance in a tissue- and light-specific manner. While not offering the accuracy of conventional semi-quantitative or competitive RT-PCR, our method allows quick screening of large numbers of genes in a wide range of RNA samples with just a thermal cycler and standard gel analysis equipment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007523305132
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  • 29
    Digitale Medien
    Digitale Medien
    Real-time feedback scheduling of flexible manufacturing systems (1992)
    Springer
    Journal of intelligent and robotic systems 6 (1992), S. 51-63 
    Details
    ISSN: 1573-0409
    Schlagwort(e): Scheduling ; flexible manufacturing systems ; stability ; feedback
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik , Maschinenbau
    Notizen: Abstract A genetic manufacturing environment is considered. The emphasis is on small-lot, discrete, and asynchronous type of manufacturing systems rather than high volume and continuous type. Two classes of scheduling policies are proposed to render the machine stable. The policies are of feedback type. The decision is made in real-time and on-line.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00314697
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  • 30
    Digitale Medien
    Digitale Medien
    Force control of robots with nonlinearities (1992)
    Springer
    Journal of intelligent and robotic systems 6 (1992), S. 219-240 
    Details
    ISSN: 1573-0409
    Schlagwort(e): Force control ; slip-stick friction ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik , Maschinenbau
    Notizen: Abstract This paper explores two practical issues related to the force control of manipulators. The first issue examined is how system stability is effected by commonly occurring manipulator nonlinearities, such as sampled-data, control signal saturation and slip-stick friction. It is shown that discretely implemented force control algorithms can drive the feedback force controlled manipulator into a limit cycle, even for a very small sampling period that by far satisfies Shannon's sampling theorem. The bounds of stability are enhanced by the presence of control signal saturation and slip stick friction. The second issue investigated is the inclusion of a high gain inner position loop as a means to minimize the unpredictability in the steady state error due to slip-stick friction. In order to support the theoretical conclusions, experiments were performed with the PUMP 560 industrial robot testbed facility developed at Colorado State University.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00248017
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  • 31
    Digitale Medien
    Digitale Medien
    Autonomous Intelligent Cruise Control with Actuator Delays (1998)
    Springer
    Journal of intelligent and robotic systems 23 (1998), S. 27-43 
    Details
    ISSN: 1573-0409
    Schlagwort(e): autonomous control ; actuator delays ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik , Maschinenbau
    Notizen: Abstract In this paper, we consider the control design problem of vehicle following systems with actuator delays. An upper bound for the time delays is first constructed to guarantee the vehicle stability. Second, sufficient conditions are presented to avoid slinky-effects in the vehicle following. Next, zero steady state achieved by the proposed controller is proven. Finally, simulations are given to examine our claims.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007946007601
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  • 32
    Digitale Medien
    Digitale Medien
    A structural approach to robust stability of polynomials (1992)
    Springer
    Multidimensional systems and signal processing 3 (1992), S. 421-425 
    Details
    ISSN: 1573-0824
    Schlagwort(e): Convex combination ; polynomial ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: Abstract A sufficient condition for Hurwitz stability of a convex combination of two stable polynomials is obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01940234
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  • 33
    Digitale Medien
    Digitale Medien
    Fertilizer value of pig slurries processed by the Promest procedure (1992)
    Springer
    Nutrient cycling in agroecosystems 32 (1992), S. 61-70 
    Details
    ISSN: 1573-0867
    Schlagwort(e): Pig slurry ; processing ; relative efficiency index ; N ; P ; K ; organic carbon ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract In the Netherlands, pig slurries are processed to improve their fertilizer value as well as handling and application. The relative efficiency index (REI) of N, P and K and the stability of organic carbon (C) in test products of processed pig slurries (PPS) were determined in pot and incubation experiments. The dry and easily manageable PPS were produced according to the Promest procedure. The mineral fraction of total N varied from 60 to 88%. It consisted mainly of nitrate. About 25% of the organic N was rapidly mineralized. The REI of N varied from 66 to 92%. After application REI of P from pelletized PPS varied from 41 to 62% in the first half year. The REI of P in ground but non-pelletized PPS varied from 81 to 100%. The REI of K was estimated to be 100%. PPS contained less easily decomposable organic C than untreated pig slurry due to the anaerobic digestion step in the processing procedure. About 34% of the organic C disappeared as methane and carbon dioxide during processing. It was concluded that processing improved the fertilizer value of PPS compared with that of untreated pig slurry.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01054395
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  • 34
    Digitale Medien
    Digitale Medien
    Genetic approaches to the study of mitochondrial biogenesis in yeast (1992)
    Springer
    Antonie van Leeuwenhoek 62 (1992), S. 131-153 
    Details
    ISSN: 1572-9699
    Schlagwort(e): mitochondrial DNA ; mutational analysis ; nucleo-mitochondrial interactions ; gene expression ; membrane assembly ; respiratory deficiency
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In contrast to most other organisms, the yeastSaccharomyces cerevisiae can survive without functional mitochondria. This ability has been exploited in genetic approaches to the study of mitochondrial biogenesis. In the last two decades, mitochondrial genetics have made major contributions to the identification of genes on the mitochondrial genome, the mapping of these genes and the establishment of structure-function relationships in the products they encode. In parallel, more than 200 complementation groups, corresponding to as many nuclear genes necessary for mitochondrial function or biogenesis have been described. Many of the latter are required for post-transcriptional events in mitochondrial gene expression, including the processing of mitochondrial pre-RNAs, the translation of mitochondrial mRNAs, or the assembly of mitochondrial translation products into the membrane. The aim of this review is to describe the genetic approaches used to unravel the intricacies of mitochondrial biogenesis and to summarize recent insights gained from their application.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00584467
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  • 35
    Digitale Medien
    Digitale Medien
    Renormalization of the Chapman–Enskog Expansion: Isothermal Fluid Flow and Rosenau Saturation (1998)
    Springer
    Journal of statistical physics 91 (1998), S. 285-305 
    Details
    ISSN: 1572-9613
    Schlagwort(e): Chapman–Enskog expansion ; Burnett equation ; Boltzmann equation ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract This paper continues the author's study of procedures for rewriting the well-known Chapman–Enskog expansion used in the kinetic theory of gases. The usual Chapman–Enskog expansion, when used in isothermal fluid motion, will introduce nonlinear instability at super-Burnett order O(ε3) truncation. The procedure given here eliminates the truncation instability and produces the desired dissipation inequality.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1023048322851
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  • 36
    Digitale Medien
    Digitale Medien
    Robust Regulator for Flexible-Joint Robots Using Integrator Backstepping (1998)
    Springer
    Journal of intelligent and robotic systems 22 (1998), S. 23-38 
    Details
    ISSN: 1573-0409
    Schlagwort(e): robot dynamic model ; stiffness matrix ; constant disturbance ; integrator backstepping ; Liapunov functions ; Barbalat lemma ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik , Maschinenbau
    Notizen: Abstract A robust regulator for flexible-joint robots is proposed, which yields constant torque disturbance rejection acting on the links. The design uses the integrator backstepping technique [4,5] to cancel nonlinearities and disturbance not in the range space of the control. Stability of the closed loop system is shown using iterative Liapunov functions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007947416837
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  • 37
    Digitale Medien
    Digitale Medien
    A reinforcement connectionist approach to robot path finding in non-maze-like environments (1992)
    Springer
    Machine learning 8 (1992), S. 363-395 
    Details
    ISSN: 0885-6125
    Schlagwort(e): Connectionism ; reinforcement learning ; robot path finding ; stability ; reactive systems
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract This paper presents a reinforcement connectionist system which finds and learns the suitable situation-action rules so as to generate feasible paths for a point robot in a 2D environment with circular obstacles. The basic reinforcement algorithm is extended with a strategy for discovering stable solution paths. Equipped with this strategy and a powerful codification scheme, the path-finder (i) learns quickly, (ii) deals with continuous-valued inputs and outputs, (iii) exhibits good noise-tolerance and generalization capabilities, (iv) copes with dynamic environments, and (v) solves an instance of the path finding problem with strong performance demands.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00992702
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  • 38
    Digitale Medien
    Digitale Medien
    A Reinforcement Connectionist Approach to Robot Path Finding in Non-Maze-Like Environments (1992)
    Springer
    Machine learning 8 (1992), S. 363-395 
    Details
    ISSN: 0885-6125
    Schlagwort(e): Connectionism ; reinforcement learning ; robot path finding ; stability ; reactive systems
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract This paper presents a reinforcement connectionist system which finds and learns the suitable situation-action rules so as to generate feasible paths for a point robot in a 2D environment with circular obstacles. The basic reinforcement algorithm is extended with a strategy for discovering stable solution paths. Equipped with this strategy and a powerful codification scheme, the path-finder (i) learns quickly, (ii) deals with continuous-valued inputs and outputs, (iii) exhibits good noise-tolerance and generalization capabilities, (iv) copes with dynamic environments, and (v) solves an instance of the path finding problem with strong performance demands.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1022684924132
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  • 39
    Digitale Medien
    Digitale Medien
    Stimulatory effect of calcium administration on regucalcin mRNA expression is attenuated in the kidney cortex of rats ingested with saline (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 275-281 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; gene expression ; calmodulin ; spontaneous hypertensive rats ; rat kidney cortex
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats ingested with saline was investigated. The alteration in regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb of open reading frame). Rats were freely given saline as drinking water for 7 days. Regucalcin mRNA levels in the kidney cortex were suppressed by saline ingestion. When calcium chloride (10 mg Ca/100 g body weight) was intraperitoneally administered to rats ingested with saline for 7 days, the effect of calcium administration to increase regucalcin mRNA levels was weakened by saline ingestion. Such effect was also seen by the administration of 2.5 and 5 mg Ca/100 g. Regucalcin mRNA levels in the kidney cortex of spontaneous hypertensive rats (SHR) were not appreciably increased by the administration of calcium (10 mg/100 g). Meanwhile, calcium content in the kidney cortex was significantly elevated by the administration of calcium (10 mg/100 g) to normal rats. This increase was weakened in saline-ingested rats. Moreover, Ca2+/calmodulin-dependent protein kinase activity in the cytosol of kidney cortex was significantly decreased by saline ingestion. These results suggest the possibility that saline ingestion-induced suppression of regucalcin mRNA expression in the kidney cortex is partly involved in the attenuation of Ca2+ signalling.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006898910955
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  • 40
    Digitale Medien
    Digitale Medien
    Molecular cloning, nucleotide sequence and expression of a cDNA encoding an intracellular protein tyrosine phosphatase, PTPase-2, from mouse testis and T-cells (1992)
    Springer
    Molecular and cellular biochemistry 118 (1992), S. 91-98 
    Details
    ISSN: 1573-4919
    Schlagwort(e): mouse ; protein tyrosine phosphatase ; cDNA cloning ; nucleotide sequence ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The PTP-2 cDNA encoding an intracellular protein tyrosine phosphatase (PTPase-2) was isolated and sequenced from mouse testis and T-cell cDNA libraries. This PTP-2 cDNA was found to be homologous to human PTP-TC and rat PTP-S, and contained 1,551 nucleotides, including 1,146 nucleotides encoding 382 amino acids as well as 5′ (61 nucleotides) and 3′ (344 nucleotides) non-coding regions. Northern blot analysis indicated that PTP-2 mRNA of 1.9 Kb was most abundant in testis and kidney, although it was also present in spleen, muscle, liver, heart and brain.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00249698
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  • 41
    Digitale Medien
    Digitale Medien
    Protein tyrosine phosphatase gene expression analysis in Swiss 3T3 fibroblasts (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 157-162 
    Details
    ISSN: 1573-4919
    Schlagwort(e): protein tyrosine phosphatases ; gene expression ; degenerate deoxyoligonucleotides ; RT-PCR ; Swiss 3T3 fibroblasts
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The aim of this study was to identify protein tyrosine phosphatases (PTPs) expressed in Swiss 3T3 fibroblasts and to examine their expression levels as well as to characterize quantitative aspects of RT-PCR based on degenerate deoxyoligonucleotides. By using an RT-PCR assay based on degenerate deoxyoligonucleotide primers, expression of mRNAs for two cytoplasmic- and six transmembrane-type PTPs in Swiss 3T3 cells was detected. The sequences of two of them are new. Among nine analyzed PTPs expressed to widely varied extends, only three have mRNA levels high enough to be seen on Northern blots with 10 µg of total RNA per lane. The frequencies with which the examined PTPs are represented among the PCR amplification products, correlate stronger with the primer fidelity, defined as the number of mismatches between the primer- and the cDNA target-sequences, rather than with the PTP expression levels. In conclusion, an RT-PCR assay based on degenerate primers can be successfully used to sample the expressed PTPs and to identify new members of this gene family. However, reliable quantification of their mRNA levels can only be achieved using the classical approaches, like Northern, RNase protection assay or non-degenerate quantitative RT-PCR.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006897629337
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  • 42
    Digitale Medien
    Digitale Medien
    Gene expression after short periods of coronary occlusion (1998)
    Springer
    Molecular and cellular biochemistry 186 (1998), S. 43-51 
    Details
    ISSN: 1573-4919
    Schlagwort(e): myocardial ischemia ; gene expression ; growth factors ; phospholamban ; calsequestrin heat shock proteins ; preconditioning ; stunning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Brief periods of coronary occlusion render the affected myocardium more tolarant to the otherwise devastating effects of long coronary occlusion. Besides this phenomena, called ischemic preconditioning, short periods of ischemia cause a regional dysfunction, namely myocardial stunning. The molecular mechanisms of both syndromes are not very well understood. We therefore investigated the expression of genes which may be involved in cardioprotection or repair processes.Using our porcine model of ischemia and reperfusion we were able to show an induction of genes coding for transcription factors (proto-oncogenes), for proteins involved in repair processes (heat shock genes), for proteins implicated in the calcium homeostasis (calcium-handling genes) and for growth factors. We could show that the increased mRNA levels are due to an enhanced transcriptional activity and not to a prolonged half-life of the transcripts. The angiogenic growth factor vascular endothelial growth factor (VEGF) represents an exception. It exhibits - in addition to a HIF-motif (Hypoxia Inducible Factor) in its promoter/enhancer - a protein binding region in its 3′ UTR which when occupied renders the mRNA more stable. However to what extent the expression of the distinct genes contributes to the cardioprotective effect of ischemic preconditioning or myocardial stunning can only be presumed. Increased mRNA stability can be confered via adenosine which is produced during ischemia by ATP-breakdown. The demasking of unknown genes - via differential display reverse transcription polymerase chain reaction (DDRT-PCR) - should provide a more comprehensive view of the mechanisms underlying both processes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006853432678
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  • 43
    Digitale Medien
    Digitale Medien
    Resilience in the Dynamics of Economy-Environment Systems (1998)
    Springer
    Environmental and resource economics 11 (1998), S. 503-520 
    Details
    ISSN: 1573-1502
    Schlagwort(e): biodiversity ; dynamics ; resilience ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik , Wirtschaftswissenschaften
    Notizen: Abstract The ecological concept of resilience has begun to inform analysis of change in economy-environment systems. The linkages between resilience and the stability of dynamical systems are discussed, along with its role in understanding of the evolution of such systems. Particular linkages discussed include those between resilience, biodiversity and the sustainability of alternative states. Recent developments in modelling the resilience of joint economy-environment systems suggest the advantages of analysing change in the system as a Markov process, the transition probabilities between states offering a natural measure of the resilience of the system in such states. It is argued that this ‘emergent property’ of the collaboration between ecology and economics has far-reaching implications for the way we think about, model and manage the environmental sustainability of economic development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008255614276
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  • 44
    Digitale Medien
    Digitale Medien
    Expression of calcium-binding protein regucalcin mRNA in fetal rat liver is stimulated by calcium administration (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 283-287 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; gene expression ; fetal development ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The expression of hepatic calcium-binding protein regucalcin mRNA in fetal rats was investigated. The alteration in regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin cDNA (0.9 kb with complete open reading frame). Hepatic regucalcin mRNA levels were progressively increased with fetal development; the mRNA was clearly expressed at 15 and 21 days of pregnancy but only slightly at the 8 days. Meanwhile, β-actin mRNA levels in the fetal liver were remarkable at 8 and 15 days of pregnancy. The fetal liver regucalcin mRNA levels at 15 days of pregnancy were significantly decreased by overnight-fasting of maternal rats. The oral administration of calcium chloride (50 mg Ca/100 g body weight) to maternal rats at 15 days of pregnancy caused a remarkable elevation (about 2 fold) of regucalcin mRNA levels in the fetal liver; this increase was seen 60 and 180 min after the calcium administration. After birth, regucalcin mRNA was increasingly expressed in the livers of newborn and weanling rats, while hepatic β-actin mRNA expression was not appreciably altered with increasing ages. These findings demonstrate that the expression of hepatic regucalcin mRNA is increased with fetal development, and that the gene expression may be stimulated by the ingestion of dietary calcium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006803211864
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  • 45
    Digitale Medien
    Digitale Medien
    Enhanced NOR-1 gene expression by exposure of Chinese hamster cells to high-density 50 Hz magnetic fields (1998)
    Springer
    Molecular and cellular biochemistry 181 (1998), S. 191-195 
    Details
    ISSN: 1573-4919
    Schlagwort(e): extremely low frequency magnetic fields ; gene expression ; neuron derived orphan receptor-1 ; signal transduction ; Chinese hamster ovary K1 cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Enhanced expression of neuron derived orphan receptor (NOR-1) gene was observed by exposure of Chinese hamster ovary K1 (CHO-K1) cells to an extremely low frequency magnetic field (ELFMF) of 50 Hz at 400 mT, but not at 5 mT. The enhanced expression, reaching the maximum at 6 h, was transient and reduced to the control level after exposure to 400 mT ELFMF for 24 h. The NOR-1 expression induced by treatment with forskolin and TPA was further enhanced by the simultaneous treatment with 400 mT ELFMF, in which the maximum response was at 3 h. The NOR-1 expression by these treatments was induced more earlier than that by 400 mT ELFMF alone. When cells were treated with an inhibitor of the protein kinase C (calphostin C or crocetin) and Ca2+ entry blockers (nifedipin and dantrolen) during the 400 mT ELFMF exposure, the enhanced NOR-1 expression was not observed. Exposure of CHO-K1 cells to the high-density 400 mT ELFMF may affect the signal transduction in the cells, resulting in the enhanced NOR-1 gene expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006828400868
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  • 46
    Digitale Medien
    Digitale Medien
    The molecular basis for the role of zinc in developmental biology (1998)
    Springer
    Molecular and cellular biochemistry 188 (1998), S. 41-48 
    Details
    ISSN: 1573-4919
    Schlagwort(e): zinc ; transcription factors ; gene expression ; organogenesis ; Xenopus laevis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Zinc regulates the gene expression machinery. It affects the structure of chromatin, the template function of its DNA, the activity of numerous transcription factors and of RNA polymerases. Hence, it determines both the types of mRNA transcripts synthesized and the rate of transcription itself. Alterations in one or more of these zinc dependent processes have been proposed to account for the proliferative arrest and teratology induced by zinc deficiency. To examine this proposal, studies of zinc during X. laevis development have been initiated. The kinetics of X. laevis oocyte zinc uptake and storage and of zinc utilization during embryogenesis have been examined first. Vitellogenin carries zinc into the oocyte. Ten % of the total zinc (10 ng/egg) remains within the cytosol while 90% (90 ng/egg) is stored in the yolk platelets associated with lipovitellin. The cytosolic pool is the source of the zinc for all newly formed metalloproteins involved in embryo development. The yolk platelet zinc pool is stored for later use during early metamorphosis. It is now possible to examine zinc transfer to molecules, such as e.g. transcription factors, and the role of the metal in their function in development and organogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006808119862
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  • 47
    Digitale Medien
    Digitale Medien
    Effect of 60 Hz magnetic field exposure on c-fos expression in stimulated PC12 cells (1998)
    Springer
    Molecular and cellular biochemistry 189 (1998), S. 107-111 
    Details
    ISSN: 1573-4919
    Schlagwort(e): gene expression ; electromagnetic fields ; superinduction ; anisomycin ; immediate early gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Rat pheochromocytoma PC12 cells have been treated with nerve growth factor (NGF) at final concentrations of 2, 4, 8, and 16 ng/ml, and then were exposed to 60-Hz, sinusoidal magnetic fields (MF) of 12.5, 25, 50, and 100 μT (rms) for 30 min. Transcript levels for both c-fos and glyceraldehyde-3 -phosphate dehydrogenase were determined by Northern blot analysis using 32P-labeled cDNA probes. No change in c-fos expression was measured at any condition employed. Treatment of PC12 cells with a combination of agents (NGF, forskolin, and tetradecanoylphorbol acetate [TPA]) increased c-fos expression over that detected with NGF alone. MF exposure of cells treated with the three-agent regimen produced two outcomes, either no change or a doubling of c-fos expression. In subsequent experiments, cells were treated with NGF, NGF + forskolin + TPA, or pre-treated with anisomycin and then treated with NGF + forskolin + TPA. It was determined that MF exposure, like superinduction with anisomycin, increased c-fos expression only in cultures which were not yet exhibiting maximal c-fos expression. It is hypothesized that MF exposure, like anisomycin, may alter the activity of key intracellular protein kinases.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006872309385
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  • 48
    Digitale Medien
    Digitale Medien
    Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells (1998)
    Springer
    Molecular biology reports 25 (1998), S. 13-19 
    Details
    ISSN: 1573-4978
    Schlagwort(e): gene expression ; nuclear matrix proteins ; ocular lens epithelial cells ; transcription factors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Association of transcription factors with the nuclear matrix represents a mechanism by which nuclear architecture may influence transcriptional control of gene expression. This investigation examines nuclear matrix associated proteins (NMP's) isolated from ocular lens epithelial cells by monitoring DNA binding activities using consensus oligonucleotides recognized by the transcription factors YY1, AML-1, AP-1, SP-1 and ATF. The nuclear matrix fractions tested included an immortilized human lens epithelial cell line containing the SV40 large T-antigen, and two mouse lens epithelial cell lines derived from either a normal mouse or a cataract mouse. A rabbit epidermal epithelial cell line and HeLa cells were also included in this study for comparison. The data from these experiments reveal that ubiquitously represented and tissue restricted regulatory proteins are associated with nuclear matrix of lens epithelial cells. The functional significance of the nuclear matrix association of these transcription factors remains to be determined. However, our findings raise the possibility that the transcription factors associated with the nuclear matrix could have specific roles in gene regulation and eye tissue development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006886110771
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  • 49
    Digitale Medien
    Digitale Medien
    Effect of deletions 5′ to the translation initiation sequence on the expression of an mRNA in animal cells (1992)
    Springer
    Molecular biology reports 16 (1992), S. 277-284 
    Details
    ISSN: 1573-4978
    Schlagwort(e): translational initiation ; 18S rRNA ; mRNA secondary structure ; gene expression ; initiation mutants ; β-galactosidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To learn if an mRNA·18S rRNA interaction or a special secondary structure in the mRNA start region is essential for translation in eukaryotic cells, we constructed recombinant plasmids with the SV40 early promoter 5′ to part of the Escherichia coli tuf B-lacZ gene. Deletion of bases potentially complementary to the 18S rRNA highly increased the transient β-galactosidase expressed in transfected CHO cells. Deletion of bases that fostered formation of potential hairpins with the mRNA 5′-terminus or altered the structure of the coding region reduced β-galactosidase activity suggesting that these features of the mRNA secondary structure may be essential for initiation of translation. Computer aided analysis of the potential structure of 290 mRNAs suggests these are conserved features of the initiation region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00419668
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  • 50
    Digitale Medien
    Digitale Medien
    Isolation and characterization of a cDNA that encodes ECP31, an embryogenic-cell protein from carrot (1992)
    Springer
    Plant molecular biology 19 (1992), S. 239-249 
    Details
    ISSN: 1573-5028
    Schlagwort(e): ABA ; Daucus carota ; ECP31 ; gene expression ; LEA clone ; somatic embryogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A full-length cDNA for ECP31, an embryogenic cell protein from carrot (Daucus carota L.) with a M r of 31000 (Kiyosue T, Satoh S, Kamada H, Harada H (1991) Plant Physiol 95: 1077–1083), was isolated from a cDNA library prepared from embryogenic cells using PCR-amplified DNA as a probe. The genomic Southern blot analysis revealed that there are two or three genes for ECP31 in the carrot genome. The transcripts of ECP31 accumulated in the peripheral regions of clusters of embryogenic cells and disappeared in the course of somatic embryogenesis that was induced by transfer of the embryogenic cells to auxin-free media. The cDNA encodes a polypeptide of 256 amino acids, and the calculated molecular weight of this polypeptide is 26 111. The deduced amino acid sequence shows a high degree (62.2%) of similarity to that of a protein that is abundant during late embryogenesis of cotton (LEA D34; Baker JC, Steele C, Dure III (1988) Plant Mol Biol 11: 227–291). The mRNAs for ECP31 started to accumulate in zygotic embryos at a late stage of embryogenesis but were undetectable in mature embryos within 24 h after imbibition of seeds. In dry fruits (seeds), the transcripts were detected only in zygotic embryos by in situ hybridization. The level of ECP31 transcripts increased after treatment with abscisic acid (ABA) in torpedo-shaped somatic embryos but not in seven-day-old seedlings. These results suggest that both embryo-specific factor(s) and ABA are involved in the expression of the gene for ECP31.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027345
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  • 51
    Digitale Medien
    Digitale Medien
    The heat shock response of pollen and other tissues of maize (1992)
    Springer
    Plant molecular biology 19 (1992), S. 623-630 
    Details
    ISSN: 1573-5028
    Schlagwort(e): gene expression ; heat shock ; intron ; maize ; pollen ; RNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract While a heat shock treatment of 40 °C or 45 °C induced the vegetative tissues of maize to produce the typical heat shock proteins (HSPs), germinating maize pollen exposed to the same temperatures did not synthesize these characteristic HSPs. Comparison of RNA accumulation in shoot and tassel tissue showed that mRNAs for HSP70 and HSP18 increased several-fold, reaching high levels within 1 or 2 hours. At the higher temperature of 45 °C these vegetative tissues were blocked in removal of an intron from the HSP70 mRNA precursor, which accumulated to a high level in tassel tissue. In germinating pollen exposed to heat shock, mRNAs for these HSPs were induced but accumulated only to low levels. The stressed pollen maintained high levels of RNA for α-tubulin, a representative normal transcript. It is likely that the defective heat shock response of maize pollen is due to inefficient induction of heat shock gene transcription.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00026788
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  • 52
    Digitale Medien
    Digitale Medien
    Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs (1992)
    Springer
    Plant molecular biology 19 (1992), S. 781-792 
    Details
    ISSN: 1573-5028
    Schlagwort(e): sunflower ; gene expression ; zygotic embryogenesis ; Lea proteins ; heat-shock proteins ; abscisic acid ; osmotic stress
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have cloned and sequenced three different cDNAs from sunflower seed-stored mRNA. Sequence similarities and response to heat-shock identified one of the cDNAs as a low-molecular-weight heat-shock protein (lmw-HSP). The other two clones showed significant sequence similarity to the cotton and carrot late-embryogenesis-abundant (Lea) proteins D-113 and Emb-1, respectively. The three cDNAs showed similar expression patterns during zygotic embryo development, as well as in vegetative tissues of 3-day-old seedlings in response to stress. Maximal accumulation of all three mRNAs was detected in dry seeds and during embryo mid-maturation stage, in the absence of exogenous stress. In seedlings, mRNAs accumulated to lower levels in response to osmotic stress and exogenous abscisic acid (ABA) treatments. A differential time course of response to osmotic stress was observed: lmw-HSP mRNA accumulation was induced earlier than that of Lea mRNAs. The coordinate accumulation of Lea and lmw-HSP transcripts during embryo development and in response to stress and ABA suggests the existence of common regulatory elements for Lea and lmw-HSP genes, and supports the notion that HSPs might have alternative functions in the plant cell.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027074
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  • 53
    Digitale Medien
    Digitale Medien
    Molecular analysis of a cruciferin storage protein gene family of Brassica napus (1992)
    Springer
    Plant molecular biology 19 (1992), S. 1049-1055 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Brassica napus ; rapeseed ; gene expression ; nucleotide sequence ; storage proteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have isolated a five-member gene subfamily which encodes cruciferin, a legumin-like 12S storage protein of Brassica napus L., and have analyzed the structure and expression of the family members in developing embryos. Sequence analysis has shown that the coding regions of all five genes are highly similar, with the two most divergent members of the family retaining 89% sequence identity. The analysis of this cruciferin gene family's expression indicates that the developmental pattern of expression of each gene is similar, and the steady-state mRNA levels of each gene are approximately equivalent to each other at all developmental stages.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040536
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  • 54
    Digitale Medien
    Digitale Medien
    Isolation and characterization of a tobacco gene with homology to pectate lyase which is specifically expressed during microsporogenesis (1992)
    Springer
    Plant molecular biology 20 (1992), S. 493-502 
    Details
    ISSN: 1573-5028
    Schlagwort(e): gene expression ; microsporogenesis ; Nicotiana tabacum ; pectate lyase ; pollen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A genomic clone has been isolated which contains an open reading frame of 1191 bp interrupted by two small introns. The ORF has been sequenced and the transcriptional start determined. The predicted amino acid sequence shows homology to the deduced amino acid sequences of two pollen-specific pectate lyase genes identified in tomato. The genomic clone was isolated using a partial cDNA clone, TP10, which had been isolated from a Nicotiana tabacum pollen cDNA library by means of differential screening. TP10 has been fully sequenced and contains an open reading frame of 792 bp which shows 96% homology to the ORF in the genomic clone. The transcript corresponding to TP10 is maximally expressed late in pollen development, and has not been detected in vegetative tissues.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040608
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  • 55
    Digitale Medien
    Digitale Medien
    Differential accumulation of mRNAs encoding extracellular and intracellular PR proteins in tomato induced by virulent and avirulent races of Cladosporium fulvum (1992)
    Springer
    Plant molecular biology 20 (1992), S. 513-527 
    Details
    ISSN: 1573-5028
    Schlagwort(e): β-1,3-glucanase ; gene expression ; pathogenesis-related proteins ; plant-fungus interaction ; protein P14
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Tomato leaves infected by the fungal pathogen Cladosporium fulvum contain several types of intracellular and extracellular pathogenesis-related (PR) proteins. Previously, we reported the purification and serological characterization of five extracellular PR proteins: P2, P4, P6, a chitinase and a β-1,3-glucanase [22, 23]. Here we describe the purification of a basic intracellular 33 kDa β-1,3-glucanase and the isolation and characterization of cDNA clones encoding the two extracellular P14 isomers P4 and P6, the extracellular acidic β-1,3-glucanase and a basic 35 kDa β-1,3-glucanase, different from the purified 33 kDa protein. Southern blot analysis demonstrated that tomato PR proteins are not encoded by large gene families, as is the case in tobacco. The number of genes corresponding to each protein was estimated to vary between one and three. A northern blot analysis indicated that the mRNAs for the extracellular PR proteins (P4, P6 and acidic β-1,3-glucanase) accumulate to similar levels in compatible and incompatible tomato-C. fulvum interactions, although the maximum level of expression is reached much faster in the incompatible interaction. On the other hand, the mRNA for the basic 35 kDa β-1,3-glucanase is induced rapidly to high levels in both interactions, but declines in time to background levels only in the incompatible interaction. The relevance of this difference in relation to plant defence is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040610
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  • 56
    Digitale Medien
    Digitale Medien
    cDNA cloning of a tetraubiquitin gene, and expression of ubiquitin-containing transcripts, in aleurone layers of Avena fatua (1992)
    Springer
    Plant molecular biology 20 (1992), S. 753-758 
    Details
    ISSN: 1573-5028
    Schlagwort(e): aleurone ; Avena fatua ; cDNA nucleotide sequence ; gene expression ; gibberellin ; polyubiquitin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A λgt11 cDNA library, constructed from poly(A)+ mRNA isolated from Avena fatua aleurone layers incubated with 1 μM gibberellin A1 (GA1) for 4 days, was screened with an anti-idiotypic antiserum raised against the GA-specific monoclonal antibody MAC 182. One positive clone was isolated, sequenced and shown to encode a tetraubiquitin based on the deduced amino acid sequence. This polyubiquitin cDNA exhibited a high degree of homology to a cloned wheat hexaubiquitin in its 3′-non-coding region. Analysis of total RNA isolated from A. fatua aleurone layers, treated without or with a range of concentrations of GA1 from 10-11 to 10-6 M, by northern blotting using the cDNA probe revealed 8 different ubiquitin-containing transcript classes all of which are constitutively expressed in aleurone and are regulated by GA1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046461
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  • 57
    Digitale Medien
    Digitale Medien
    Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses (1992)
    Springer
    Plant molecular biology 20 (1992), S. 897-910 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Cell division ; gene expression ; Nicotiana sylvestris ; protoplast ; stress ; ubiquitin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027161
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  • 58
    Digitale Medien
    Digitale Medien
    Translation controls the expression level of a chimaeric reporter gene (1992)
    Springer
    Plant molecular biology 20 (1992), S. 921-938 
    Details
    ISSN: 1573-5028
    Schlagwort(e): β-D-glucuronidase ; mannopine synthase promoter ; Agrobacterium ; gene expression ; initiation of translation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Transcriptional and translational fusions between the reading frame of the β-D-glucuronidase gene (gusA) and the 2′ as well as the 1′ promoter of mannopine synthase (mas), a TR locus of Agrobacterium tumefaciens, were made. The expression of these constructs was studied in the transgenic F1 offspring of independent tobacco transformants at the protein level by assaying for GUS activity and western blot analysis of the GUS protein and at the steady-state mRNA level. In leaves, stems and roots no correlation was found between steady-state levels of GUS mRNA and enzyme activity. In older tissues significantly higher GUS activities were found. This is explained by the stable character of the GUS protein together with an accumulation of protein upon ageing. Three to ten times higher GUS activities were found for in vitro grown plants than for greenhouse-grown plants of the same offspring, despite similar levels of GUS mRNA. Roots from in vitro grown plants display three to ten times higher GUS activities than stems and leaves. In transgenic plants grown in vitro, containing a translational fusion with two AUGs in phase, the initiation of translation in leaf material occurred at both AUGs. Initiation of translation at the first AUG, however, was ten times more frequent. In contrast, initiation in roots from in vitro grown plants occurred exclusively at the second AUG.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00027163
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  • 59
    Digitale Medien
    Digitale Medien
    A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome (1992)
    Springer
    Plant molecular biology 20 (1992), S. 1203-1207 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Agrobacterium ; binary vector ; CaMV 35S ; gene expression ; β-glucuronidase ; Nicotiana plumbaginifolia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A versatile gene expression cartridge and binary vector system was constructed for use in Agrobacterium-mediated plant transformation. The expression cartridge of the primary cloning vector, pART7, comprises of cauliflower mosaic virus Cabb B-JI isolate 35S promoter, a multiple cloning site and the transcriptional termination region of the octopine synthase gene. The entire cartridge can be removed from pART7 as a Not I fragment and introduced directly into the binary vector, pART27, recombinants being selected by blue/white screening for β-galactosidase. pART27 carries the RK2 minimal replicon for maintenance in Agrobacterium, the ColE1 origin of replication for high-copy maintenance in Escherichia coli and the Tn7 spectinomycin/streptomycin resistance gene as a bacterial selectable marker. The organisational structure of the T-DNA of pART27 has been constructed taking into account the right to left border, 5′ to 3′ model of T-DNA transfer. The T-DNA carries the chimaeric kanamycin resistance gene (nopaline synthase promoter-neomycin phosphotransferase-nopaline synthase terminator) distal to the right border relative to the lacZ′ region. Utilisation of these vectors in Agrobacterium-mediated transformation of tobacco demonstrated efficient T-DNA transfer to the plant genome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00028910
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  • 60
    Digitale Medien
    Digitale Medien
    The early genetic response to light in the green unicellular alga Chlamydomonas eugametos grown under light/dark cycles involves genes that represent direct responses to light and photosynthesis (1992)
    Springer
    Plant molecular biology 18 (1992), S. 429-445 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Chlamydomonas eugametos ; chlorophyll a/b-binding proteins ; circadian oscillator ; gene expression ; light-regulated genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In the green unicellular alga Chlamydomonas eugametos, cellular division is readily synchronized by light/dark cycles. Under these conditions, light initiates photosynthetic growth in daughter cells and begins the G1 phase. Genes whose expression is regulated upon illumination are likely to be important mechanisms controlling cell proliferation. To identify some of those genes, two cDNA libraries were prepared with poly(A)+ extracted from cells either stimulated with light for 1 h or held in darkness (quiescent cells) during the same period. To restrict our analysis to those genes that are part of the primary response, cells were incubated in presence of cycloheximide. Differential screening of approximately 40 000 clones in each library revealed 44 clones which hybridize preferentially with a [32P] cDNA probe derived from RNA of light-stimulated cells and 15 clones which react selectively with a [32P] cDNA probe synthesized from poly(A)+ RNA of quiescent cells. Cross-hybridization of these clones identified 4 independent sequences in the light-induced (LI) collection and 2 in the uninduced (LR) library. Four of these cDNAs correspond to mRNAs that are positively or negatively regulated upon activation of photosynthesis. One clone represents a mRNA that accumulates transitorily at both transitions. Finally, LI818 cDNA identifies a new chlorophyll a/b-binding (cab) gene family whose mRNA accumulation is controlled by light and a circadian oscillator. The endogenous timing system controls LI818 mRNA accumulation so that it precedes the onset of illumination by a few hours. On the other hand, light affects LI818 mRNA levels independently of active photosynthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040659
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  • 61
    Digitale Medien
    Digitale Medien
    Accumulation of wound-inducible ACC synthase transcript in tomato fruit is inhibited by salicylic acid and polyamines (1992)
    Springer
    Plant molecular biology 18 (1992), S. 477-487 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Tomato ; gene expression ; wounding ; ethylene ; glycine-rich protein ; rRNA ; polyamines
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Regulation of wound-inducible 1-aminocyclopropane-1-carboxylic acid (ACC) synthase expression was studied in tomato fruit (Lycopersicon esculentum cv. Pik-Red). A 70 base oligonucleotide probe homologous to published ACC synthase cDNA sequences was successfully used to identify and analyze regulation of a wound-inducible transcript. The 1.8 kb ACC synthase transcript increased upon wounding the fruit as well as during fruit ripening. Salicylic acid, an inhibitor of wound-responsive genes in tomato, inhibited the wound-induced accumulation of the ACC synthase transcript. Further, polyamines (putrescine, spermidine and spermine) that have anti-senescence properties and have been shown to inhibit the development of ACC synthase activity, inhibited the accumulation of the wound-inducible ACC synthase transcript. The inhibition by spermine was greater than that caused by putrescine or spermidine. The transcript level of a wound-repressible glycine-rich protein gene and that of the constitutively expressed rRNA were not affected as markedly by either salicylic acid or polyamines. These data suggest that salicylic acid and polyamines may specifically regulate ethylene biosynthesis at the level of ACC synthase transcript accumulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040664
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  • 62
    Digitale Medien
    Digitale Medien
    Differential gene expression during germination and after the induction of adventitious bud formation in Norway spruce embryos (1992)
    Springer
    Plant molecular biology 18 (1992), S. 713-724 
    Details
    ISSN: 1573-5028
    Schlagwort(e): adventitious buds ; cDNA cloning ; cytokinin ; gene expression ; germination ; Norway spruce
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A pulse treatment of embryos of Norway spruce with cytokinin suppresses germinative development and induces the coordinate formation of adventitious buds from subepidermal cell layers. To analyse the patterns of gene expression associated with germination and the alterations induced by the bud induction treatment, we have isolated cDNA clones corresponding to genes that are differentially expressed in cytokinin-treated and untreatedin vitro germinating embryos. One category of 14 clones hybridized to transcripts that were abundant specifically during germination. The expression of 8 of these genes was reduced by the bud induction treatment. Four clones, including one identified as a histone H2A gene, recognized transcripts that showed an increased abundance in bud-induced versusin vitro germinating embryos. A second category of 13 clones hybridized to transcripts that increased in abundance during post-germinative development of the seedling. Among these a subset of 8 clones, including an α-tubulin clone, corresponds to genes suppressed by the bud induction treatment, whereas 5 clones, including a gene with sequence similarity to polyubiquitin, were unaffected by the treatment. One clone hybridized to a message abundant in the seed, during early germination as well as in the vegetative bud, and showed 60% partial sequence identity to a barley (1→3)-β-glucanase gene. Genes expressed exclusively in bud-induced orin vitro germinating embryos were not found. The results show that a major difference in gene expression between treated and untreated embryos is related to the shift from extensive cell proliferation to elongation and differentiation that occurs at the transition from germination to post-germinative development, and which is suppressed in the bud-induced embryos.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020013
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  • 63
    Digitale Medien
    Digitale Medien
    A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants (1992)
    Springer
    Plant molecular biology 18 (1992), S. 749-757 
    Details
    ISSN: 1573-5028
    Schlagwort(e): salt stress ; stem-specific expression ; lipid transfer protein ; cDNA sequence ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A full-length tomato cDNA clone, TSW12, which is developmentally and environmentally regulated, has been isolated and characterized. TSW12 mRNA is accumulated during tomato seed germination and its level increases after NaCl treatment or heat shock. In mature plants, TSW12 mRNA is only detected upon treatment with NaCl, mannitol or ABA and its expression mainly occurs in stems. The nucleotide sequence of TSW12 includes an open reading frame coding for a basic protein of 114 amino acids; the first 23 amino acids exhibit the sequence characteristic of a signal peptide. The high similarity between the TSW12-deduced amino acid sequence and reported lipid transfer proteins suggests that TSW12 encodes a lipid transfer protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020016
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  • 64
    Digitale Medien
    Digitale Medien
    Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts (1992)
    Springer
    Plant molecular biology 18 (1992), S. 815-818 
    Details
    ISSN: 1573-5028
    Schlagwort(e): expression cassette ; gene expression ; protoplasts ; translation initiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Expression cassettes containing a duplicated cauliflower mosaic virus (CaMV) 35S promoter fused to a polylinker preceded by the CCACCATGG and AACAATGG sequences were constructed. These two sequences correspond to the consensus sequences around the translation start codons in vertebrates and plants respectively. Translational fusions were made with the β-glucuronidase-coding sequence and transient expression was recorded in tobacco mesophyll protoplasts. Approximately three times more GUS activity was found in protoplasts incubated with the constructs harbouring translational fusions as compared to a control harbouring a transcriptional fusion. No significant difference was observed between GUS activities obtained with the two consensus sequences.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020027
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  • 65
    Digitale Medien
    Digitale Medien
    Structure of a rice β-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors (1992)
    Springer
    Plant molecular biology 18 (1992), S. 33-45 
    Details
    ISSN: 1573-5028
    Schlagwort(e): glucanase ; gene expression ; pathogenesis response ; stress response ; plant growth regulators ; gene family
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A rice β-glucanase gene was sequenced and its expression analyzed at the level of mRNA accumulation. This gene (Gns1) is expressed at relatively low levels in germinating seeds, shoots, leaves, panicles and callus, but it is expressed at higher levels in roots. Expression in the roots appears to be constitutive. Shoots expressGns1 at much higher levels when treated with ethylene, cytokinin, salicylic acid, and fungal elicitors derived from the pathogenSclerotium oryzae or from the non-pathogenSaccharomyces cereviseae. Shoots also expressGns1 at higher levels in response to wounding. Expression in the shoots is not significantly affected by auxin, gibberellic acid or abscisic acid. The β-glucanase shows 82% amino acid similarity to the barley 1,3;1,4-β-D-glucanases, and from hybridization studies it is the β-glucanase gene in the rice genome closest to the barley 1,3;1,4-β-glucanase EI gene. The mature peptide has a calculated molecular mass of 32 kDa. The gene has a large 3145 bp intron in the codon for the 25th amino acid of the signal peptide. The gene exhibits a very strong codon bias of 99% G+C in the third position of the codon in the mature peptide coding region, but only 61% G+C in the signal peptide region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00018454
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  • 66
    Digitale Medien
    Digitale Medien
    Nucleotide sequence of a tobacco cDNA encoding plastidic glutamine synthetase and light inducibility, organ specificity and diurnal rhythmicity in the expression of the corresponding genes of tobacco and tomato (1992)
    Springer
    Plant molecular biology 19 (1992), S. 367-379 
    Details
    ISSN: 1573-5028
    Schlagwort(e): cDNA sequence ; gene expression ; glutamine synthetase ; phytochrome ; Solanaceae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A full-length cDNA encoding glutamine synthetase (GS) was cloned from a λgt10 library of tobacco leaf RNA, and the nucleotide sequence was determined. An open reading frame accounting for a primary translation product consisting of 432 amino acids has been localized on the cDNA. The calculated molecular mass of the encoded protein is 47.2 kDa. The predicted amino acid sequence of this precursor shows higher homology to GS-2 protein sequences from other species than to a leaf GS-1 polypeptide sequence, indicating that the cDNA isolated encodes the chloroplastic isoform (GS-2) of tobacco GS. The presence of C-and N-terminal extensions which are characteristic of GS-2 proteins supports this conclusion. Genomic Southern blot analysis indicated that GS-2 is encoded by a single gene in the diploid genomes of both tomato and Nicotiana sylvestris, while two GS-2 genes are very likely present in the amphidiploid tobacco genome. Western blot analysis indicated that in etiolated and in green tomato cotyledons GS-2 subunits are represented by polypeptides of similar size, while in green tomato leaves an additional GS-2 polypeptide of higher apparent molecular weight is detectable. In contrast, tobacco GS-2 is composed of subunits of identical size in all organs examined. GS-2 transcripts and GS-2 proteins could be detected at high levels in the leaves of both tobacco or tomato. Lower amounts of GS-2 mRNA were detected in stems, corolla, and roots of tomato, but not in non-green organs of tobacco. The GS-2 transcript abundance exhibited a diurnal fluctuation in tomato leaves but not in tobacco leaves. White or red light stimulated the accumulation of GS-2 transcripts and GS-2 protein in etiolated tomato cotyledons. Far-red light cancelled this stimulation. The red light response of the GS-2 gene was reduced in etiolated seedlings of the phytochrome-deficient aurea mutant of tomato. These results indicate a phytochrome-mediated light stimulation of GS-2 gene expression during greening in tomato.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023384
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  • 67
    Digitale Medien
    Digitale Medien
    Structure of the pine (Pinus thunbergii) chlorophyll a/b-binding protein gene expressed in the absence of light (1992)
    Springer
    Plant molecular biology 19 (1992), S. 405-410 
    Details
    ISSN: 1573-5028
    Schlagwort(e): cab gene ; chlorophyll a/b-binding protein ; gymnosperm ; gene expression ; pine (Pinus thunbergii)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thunbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The cab-6 gene is expressed in dark-grown seedlings at a very high level, differing from angiosperm cab genes which are induced by light. The cab-6 gene typifies the coniferous plant cab genes in light-independent gene expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023388
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  • 68
    Digitale Medien
    Digitale Medien
    Pea dehydrins: identification, characterisation and expression (1992)
    Springer
    Plant molecular biology 19 (1992), S. 1031-1044 
    Details
    ISSN: 1573-5028
    Schlagwort(e): ABA ; dehydrin ; gene expression ; pea (Pisum sativum L.) ; seed development ; water stress
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An antiserum raised against dehydrin from maize (Zea mays) recognised several polypeptides in extracts of pea (Pisum sativum) cotyledons. A cDNA expression library was prepared from mRNA of developing cotyledons, screened with the antiserum and positive clones were purified and characterised. The nucleotide sequence of one such clone, pPsB12, contained an open reading frame which would encode a polypeptide with regions of significant amino acid sequence similarity to dehydrins from other plant species. The deduced amino acid sequence of the pea dehydrin encoded by B12 is 197 amino acids in length, has a high glycine content (25.9%), lacks tryptophan and is highly hydrophilic. The polypeptide has an estimated molecular mass of 20.4 kDa and pI=6.4. An in vitro synthesised product from the clone comigrates with one of the in vivo proteins recognised by the antiserum. A comparison of the pea dehydrin sequence with sequences from other species revealed conserved amino acid regions: an N-terminal DEYGNP and a lysine-rich block (KIKEKLPG), both of which are present in two copies. Unexpectedly, pea dehydrin lacks a stretch of serine residues which is conserved in other dehydrins. B12 mRNA and dehydrin proteins accumulated in dehydration-stressed seedlings, associated with elevated levels of endogenous abscisic acid (ABA). Applied ABA induced expression of dehydrins in unstressed seedlings. Dehydrin expression was rapidly reversed when seedlings were removed from the stress or from treatment with ABA and placed in water. During pea cotyledon development, dehydrin mRNA and proteins accumulated in mid to late embryogenesis. Dehydrin proteins were some of the most actively synthesised at about the time of maximum fresh weight and represent about 2% of protein in mature cotyledons.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040534
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  • 69
    Digitale Medien
    Digitale Medien
    Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression (1992)
    Springer
    Plant molecular biology 18 (1992), S. 1-11 
    Details
    ISSN: 1573-5028
    Schlagwort(e): betaine aldehyde dehydrogenase ; gene expression ; glycine betaine ; osmotic stress ; salt tolerance ; sugar beet
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Members of the Chenopodiaceae, such as sugar beet and spinach, accumulate glycine betaine in response to salinity or drought stress. The last enzyme in the glycine betaine biosynthetic pathway is betaine aldehyde dehydrogenase (BADH). In sugar beet the activity of BADH was found to increase two- to four-fold in both leaves and roots as the NaCl level in the irrigation solution was raised from 0 to 500 mM. This increase in BADH activity was paralleled by an increase in level of translatable BADH mRNA. Several cDNAs encoding BADH were cloned from a λgt10 libary representing poly(A)+ RNA from salinized leaves of sugar beet plants, by hybridization with a spinach BADH cDNA. Three nearly full-length cDNA clones were confirmed to encode BADH by their nucleotide and deduced amino acid sequence identity to spinach BADH; these clones showed minor nucleotide sequence differences consistent with their being of two different BADH alleles. The clones averaged 1.7 kb and contained an open reading frame predicting a polypeptide of 500 amino acids with 83% identity to spinach BADH. RNA gel blot analysis of total RNA showed that salinization to 500 mM NaCl increased BADH mRNA levels four-fold in leaves and three-fold in the taproot. DNA gel blot analyses indicated the presence of at least two copies of BADH in the haploid sugar beet genome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00018451
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  • 70
    Digitale Medien
    Digitale Medien
    Cytoplasmic ribosomal protein S15a from Brassica napus: Molecular cloning and developmental expression in mitotically active tissues (1992)
    Springer
    Plant molecular biology 18 (1992), S. 909-919 
    Details
    ISSN: 1573-5028
    Schlagwort(e): ribosomal protein S15a ; cDNA clones ; rapessed ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have isolated two cDNA clones which appear to encode the 40S ribosomal subunit protein S15a from Brassica napus (oilseed rape). The open reading frame in both clones contains 390 bases, encoding a deduced polypeptide sequence of 130 amino acids (100% homology between clones) with 76% sequence identity to the N-terminal 37 amino acids of the rat ribosomal protein S15a and 80% identity to the S24 polypeptide of yeast. Both the yeast and rapeseed proteins have a net positive charge of +9 and the rapeseed S15a protein has a molecular mass of 14778 Da compared to 14762 Da for the yeast protein. The rapeseed ribosomal protein S15a is encoded by a small multi-gene family with at least two actively transcribed members. A single transcript of ca. 1.0 kb, corresponding to ribosomal protein S15a, is abundant in actively dividing tissues such as apical meristem, flower buds and young leaves and less abundant in mature stem and fully expanded leaves.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00019205
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  • 71
    Digitale Medien
    Digitale Medien
    Characterization and expression of U1snRNA genes from potato (1992)
    Springer
    Plant molecular biology 19 (1992), S. 959-971 
    Details
    ISSN: 1573-5028
    Schlagwort(e): gene expression ; gene variants ; pre-mRNA splicing ; pseudogenes ; U1 small nuclear RNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract U1 small nuclear RNAs (U1snRNAs) occur in the nucleus of plants and animals where, complexed with several proteins in the form of U1 small nuclear ribonucleoprotein particles (U1snRNPs), they play an important role in precursor messenger RNA (pre-mRNA) splicing. Ten potato U1snRNA genes have been isolated on two genomic clones illustrating the clustering of this multigene family on the potato genome. Based on both the sequence of their coding regions and upstream regulatory elements, seven of the genes are potentially functional. The other three genes were pseudogenes with defective promoter or coding region sequences. Analysis of expression of individual cloned U1snRNA genes in transfected tobacco protoplasts was impossible due to the similarity of U1snRNA sequences in tobacco. However, by marking the coding regions with oligonucleotides or constructing chimaeric genes consisting of a potato U1snRNA promoter region and maize U5snRNA coding region, three of the U1 promoter regions were shown to be transcriptionally active.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040528
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  • 72
    Digitale Medien
    Digitale Medien
    Characterization of the U3 and U6 snRNA genes from wheat: U3 snRNA genes in monocot plants are transcribed by RNa polymerase III (1992)
    Springer
    Plant molecular biology 19 (1992), S. 973-983 
    Details
    ISSN: 1573-5028
    Schlagwort(e): gene expression ; promoter specificity ; snRNA gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have demonstrated recently that the genes encoding the U3 small nuclear RNA (snRNA) in dicot plants are transcribed by RNA polymerase III (pol III), and not RNA polymerase II (pol II) as in all other organisms studied to date. The U3 gene was the first example of a gene transcribed by different polymerases in different organisms. Based on phylogenetic arguments we proposed that a polymerase specificity change of the U3 snRNA gene promoter occurred during plant evolution. To map such an event we are examining the U3 gene polymerase specificity in other plant species. We report here the characterization of a U3 gene from wheat, a monocot plant. This gene contains the conserved promoter elements, USE and TATA, in a pol III-specific spacing seen also in a wheat U6 snRNA gene characterized in this report. Both the U3 and the U6 genes possess typical pol III termination signals but lack the cis element, responsible for 3′-end formation, found in all plant pol II-specific snRNA genes. In addition, expression of the U3 gene in transfected maize protoplasts is less sensitive to α-amanitin than a pol II-transcribed U2 gene. Based on these data we conclude that the wheat U3 gene is transcribed by pol III. This observation suggests that the postulated RNA polymerase specificity switch of the U3 gene took place prior to the divergence of angiosperm plants into monocots and dicots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040529
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  • 73
    Digitale Medien
    Digitale Medien
    Molecular cloning and expression of the gene encoding ADP-glucose pyrophosphorylase from the cyanobacteriumAnabaena sp. strain PCC 7120 (1992)
    Springer
    Plant molecular biology 20 (1992), S. 37-47 
    Details
    ISSN: 1573-5028
    Schlagwort(e): ADP-glucose pyrophosphorylase ; Anabaena 7120 ; Escherichia coli ; gene cloning ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Previous studies have indicated that ADP-glucose pyrophosphorylase (ADPGlc PPase) from the cyanobacteriumAnabaena sp. strain PCC 7120 is more similar to higher-plant than to enteric bacterial enzymes in antigenicity and allosteric properties. In this paper, we report the isolation of theAnabaena ADPGlc PPase gene and its expression inEscherichia coli. The gene we isolated from a genomic library utilizes GTG as the start codon and codes for a protein of 48347 Da which is in agreement with the molecular mass determined by SDS-PAGE for theAnabaena enzyme. The deduced amino acid sequence is 63, 54, and 33% identical to the rice endosperm small subunit, maize endosperm large subunit, and theE. coli sequences, respectively. Southern analysis indicated that there is only one copy of this gene in theAnabaena genome. The cloned gene encodes an active ADPGlc PPase when expressed in anE. coli mutant strain AC70R1-504 which lacks endogenous activity of the enzyme. The recombinant enzyme is activated and inhibited primarily by 3-phosphoglycerate and Pi, respectively, as is the nativeAnabaena ADPGlc PPase. Immunological and other biochemical studies further confirmed the recombinant enzyme to be theAnabaena enzyme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00029147
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  • 74
    Digitale Medien
    Digitale Medien
    Nucleotide sequence and temporal regulation of a seed-specificBrassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase (1992)
    Springer
    Plant molecular biology 20 (1992), S. 151-155 
    Details
    ISSN: 1573-5028
    Schlagwort(e): gene expression ; desaturation ; oil synthesis ; embryogenesis ; stearoyl-acyl carrier protein desaturase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The nucleotide sequence is reported for a cDNA containing the entire coding region of a stearoyl-ACP desaturase (EC 1.14.99.6) fromBrassica napus L. cv. Jet neuf. The cDNA was obtained from a library constructed from poly(A)+ RNA purified from embryo tissue. The derived amino acid sequence demonstrates substantial similarity with those from other plant Δ9-desaturases. Comparative RNA-dot blot analyses using the Δ9-desaturase cDNA and a rapessed oleosin cDNA as probes showed that although both these transcripts were seed-specific, they exhibited distinct patterns of temporal regulation. The desaturase message was induced by 25 days after anthesis (DAA), peaking at 45 DAA but decreasing considerably thereafter. In contrast, the oleosin transcript did not increase until 45–50 DAA, reaching a peak much later at about 70 DAA.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00029157
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  • 75
    Digitale Medien
    Digitale Medien
    Temporal and spatial regulation of a novel gene in barley embryos (1992)
    Springer
    Plant molecular biology 20 (1992), S. 255-266 
    Details
    ISSN: 1573-5028
    Schlagwort(e): barley (Hordeum vulgare) ; zygotic embryogenesis ; plant development ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The temporal and spatial pattern of expression of a novel barley gene is described. The gene has been identified through the differential screening of a cDNA library constructed to poly(A)+ RNA of zygotic embryos. Transcripts corresponding to the cDNA, pZE40, become abundant in the non-axial tissues of the developing embryo within 8–10 days after anthesis, when steady-state levels are high in the scutellum, coleoptile and coleorhiza, with the exception of the scutellar epithelium. This expression pattern is maintained throughout maturation of the embryo until levels eventually decline as the grain desiccates. On germination, there is a transient re-appearance of mRNA to pZE40, with accumulation specifically restricted to the scutellum of the seedling. In situ hybridization has enabled the detection of transcripts elsewhere in the barley plant, in highly localized groups of cells. The timing and cell specificity of expression suggests the gene product is involved in the synthesis and/or transport of metabolites.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00014493
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  • 76
    Digitale Medien
    Digitale Medien
    Expression and sequence analysis of cDNAs induced during the early stages of tuberisation in different organs of the potato plant (Solanum tuberosum L.) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 641-651 
    Details
    ISSN: 1573-5028
    Schlagwort(e): S-adenosylmethionine decarboxylase ; differential screening ; gene expression ; Solanum tuberosum ; tuberisation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract cDNA clones of two genes (TUB8 and TUB13) which show a 25–30-fold increase in transcript in the stolon tip during the early stages of tuberisation, have been isolated by differential screening. These genes are also expressed in leaves, stems and roots and the expression pattern in these organs changes on tuberisation. Southern analysis shows homologous sequences in the non-tuberising wild type potato species Solanum brevidens and in Lycopersicon esculentum (tomato). Sequence analysis reveals a high degree of similarity between the TUB13 cDNA, and a human S-adenosylmethionine decarboxylase gene. The predicted TUB8 peptide sequence shows several repeats of alanine, glutamate and proline which suggests a structural role for the encoded protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046449
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  • 77
    Digitale Medien
    Digitale Medien
    Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase (1992)
    Springer
    Plant molecular biology 20 (1992), S. 663-671 
    Details
    ISSN: 1573-5028
    Schlagwort(e): PEP carboxylase ; housekeeping gene ; gene expression ; gene structure ; light-mediated activation ; Saccharum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from −1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other ‘housekeeping’ PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046451
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  • 78
    Digitale Medien
    Digitale Medien
    Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum (1992)
    Springer
    Plant molecular biology 20 (1992), S. 705-713 
    Details
    ISSN: 1573-5028
    Schlagwort(e): alanine aminotransferase ; C4 photosynthesis ; gene expression ; nucleotide sequencing ; Panicum miliaceum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have determined the nucleotide sequence of a cDNA encoding AlaAT-2, which is believed to function in the C4-pathway of Panicum miliaceum. An open reading frame (1446 bp) encodes a protein of 482 amino acid residues. The deduced amino acid sequence of AlaAT-2 shows 44.2 and 44.8% homology with the amino acid sequences of AlaATs from rat and human livers, respectively. Northern blot analysis showed that the gene encoding AlaAT-2 in mesophyll and bundle sheath cells was the same and transcribed similarly in the cells. The level of translatable mRNA for AlaAT-2 increased dramatically during greening.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046455
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  • 79
    Digitale Medien
    Digitale Medien
    Dynamical behavior of psb gene transcripts in greening wheat seedlings. I. Time course of accumulation of the psbA through psbN gene transcripts during light-induced greening (1992)
    Springer
    Plant molecular biology 20 (1992), S. 695-704 
    Details
    ISSN: 1573-5028
    Schlagwort(e): chloroplast ; gene expression ; photosystem 2 ; transcription ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The time course of the accumulation of the transcripts from 13 psb genes encoding a major part of the proteins composing photosystem II during light-induced greening of dark-grown wheat seedlings was examined focusing on early stages of plastid development (0.5 h through 72 h). The 13 genes can be divided into three groups. (1) The psbA gene is transcribed as a single transcript of 1.3 kb in the dark-grown seedlings, but its level increases 5- to 7-fold in response to light due to selective increase in RNA stability as well as in transcription activity. (2) The psbE-F-L-J operon, psbM and psbN genes are transcribed as a single transcript of 1.1 kb, two transcripts of 0.5 and 0.7 kb and a single transcript of 0.3 kb, respectively, in the dark-grown seedlings. The levels of accumulation of every transcript remain unchanged or rather decrease during plastid development under illumination. (3) The psbK-I-D-C gene cluster and psbB-H operon exhibit fairly complicated northern hybridization patterns during the greening process. When a psbC or psbD gene probe was used for northern hybridization, five transcripts differing in length were detected in the etioplasts from 5-day old dark-grown seedlings. After 2 h illumination, two new transcripts of different length appeared. Light induction of new transcripts was also observed in the psbB-H operon.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046454
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  • 80
    Digitale Medien
    Digitale Medien
    Rescue of an MMTV transgene by co-integration reveals novel locus control properties of the ovine β-lactoglobulin gene that confer locus commitment to heterogeneous tissues (1998)
    Springer
    Transgenic research 7 (1998), S. 205-212 
    Details
    ISSN: 1573-9368
    Schlagwort(e): transgenic mice ; gene expression ; mammary gland ; co-injection ; milk protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In an attempt to enhance the frequency and level of expression of a poor-performing MMTV-driven transgene, we co-integrated this construct with the ovine β-lactoglobulin (BLG) gene in transgenic mice. Seven lines of transgenic mice possessing co-integrated BLG and MMTV-RZ5 transgenes were compared with 12 lines of mice that possessed only the MMTV-RZ5 construct. Co-integration enhanced the frequency of expression in the mammary gland from two out of 12 lines for the MMTV-RZ5 transgene alone, to five out of seven when co-integrated with BLG. Surprisingly, co-integration also resulted in co-expression of the two transgenes in the salivary gland, lung and spleen in addition to the mammary gland. Furthermore, both transgenes were expressed in virgin animals, and throughout pregnancy and lactation, suggesting that the developmental regulation of the locus follows that of the MMTV-promoter. These findings represent a novel locus control property of the ovine BLG gene that confer s commitment of the locus to the mammary gland, but also to a range of heterogeneous tissues possibly defined by the second promoter at the locus
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008893030461
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  • 81
    Digitale Medien
    Digitale Medien
    Expression of Human Erythropoietin Transgenes and of the Endogenous Wap Gene in the Mammary Gland of Transgenic Rabbits during Gestation and Lactation (1998)
    Springer
    Transgenic research 7 (1998), S. 311-317 
    Details
    ISSN: 1573-9368
    Schlagwort(e): transgenic rabbits ; gene expression ; mammary gland ; erythropoietin ; WAP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An understanding of the expression of transgenes in the mammary gland during gestation and lactation is crucial for the use of transgenic mammals as bioreactors. Here we describe the temporal pattern of expression of the endogenous rabbit WAP gene and human erythropoietin (hEPO) transgenes under the control of rabbit WAP promoter and 3′ flanking sequences. The endogenous rabbit WAP gene was expressed throughout gestation including the day of mating, as well as during lactation in transgenic rabbits bearing a minigene construct. In non-pregnant cycling females, WAP expression was found independent of transgenic status; however, WAP expression was not detected in non-cycling females. The significance of this new finding is not clear at present. hEPO mRNA was detected in mammary gland biopsies from pregnant transgenic rabbits only on day 28 of gestation. During lactation, transcripts were present in mammary gland biopsy samples taken on days 0, 7, 14 and 21. A sharp decline in the levels ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008882332133
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  • 82
    Digitale Medien
    Digitale Medien
    The structure of the SF6 molecule and the SF 6 − anion excited states (1992)
    Springer
    Russian chemical bulletin 41 (1992), S. 504-510 
    Details
    ISSN: 1573-9171
    Schlagwort(e): electronic structure ; geometry optimization ; electron affinity ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The electronic and geometric structures of the ground state and a number of excited states of the SF6 molecule and the SF 6 − anion have been calculated by the discrete-variation method of the local density-functionals. The anion was found to possess a number of states stable toward the outer electron detachment, and at least one excited state stable toward dissociation. The adiabatic electron affinity (EA) was determined as 3.46 eV at the highest level of theory. This result is correlated to the high EAs of the isovalent compound SeF6 and TeF6; however, it does not agree with the presently accepted experimental estimate of 1.0 ± 0.2 eV for the SF6EA value. The basic anion configuration is octahedral with a S-F bond length of 1.717 Å. The calculated limit for the highest dissociation channel of the ground state SF 6 − → SF 5 − + F is ≈ 1.5 eV lower than the minimum of the total energy of the neutral molecule; this is in good agreement with experimental estimates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00863073
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  • 83
    Digitale Medien
    Digitale Medien
    Stability of linear time variant dynamic systems with multiple time delays (1998)
    Springer
    Acta mechanica Sinica 14 (1998), S. 274-282 
    Details
    ISSN: 1614-3116
    Schlagwort(e): time delay ; stability ; frozen time approach ; retarded dynamic systems
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract By means of the frozen time approach and the Kronecker product, two criteria of asymptotic stability are derived for the linear, time variant dynamic systems with either short time delays or with weak feedback involving arbitrary time delays, respectively. It is found that the asymptotic stability of these retarded dynamic systems is governed by the maximal and minimal singular values of the coefficient matrices and their time derivatives.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02487763
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  • 84
    Digitale Medien
    Digitale Medien
    The stability of viscous liquid jets in a swirling gas (1998)
    Springer
    Acta mechanica Sinica 14 (1998), S. 226-233 
    Details
    ISSN: 1614-3116
    Schlagwort(e): jet ; stability ; dispersion equation ; swirling gas
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract Based on the linear analysis of stability, a dispersion equation is deduced which delineates the evolution of a general 3-dimensional disturbance on the free surface of an incompressible viscous liquid jet injected into a gas with swirl. Here, the dimensionless parameterJ e is again introduced, in the meantime, another dimensionless parameterE called as circulation is also introduced to represent the relative swirling intensity. With respect to the spatial growing disturbance mode, the numerical results obtained from solving the dispersion equation reveal the following facts. First, at the same value ofE, in pace with the changing ofJ e , the variation of disturbance and the critical disturbance mode still keep the same characters. Second, the present results are the same as that of S.P. Lin whenJ e 〉1; but in the range ofJ e 〈1, it's no more the case, the swirl decreases the axisymmetric disturbance, yet increases the asymmetric disturbance, furthermore the swirl may make the character of the most unstable disturbance mode changed (axisymmetric or asymmetric); the above action of the swirl becomes much stronger whenJ e ≪1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02487757
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  • 85
    Digitale Medien
    Digitale Medien
    Relationships of differential gene expression in leaves with heterosis and heterozygosity in a rice diallel cross (1998)
    Springer
    Molecular breeding 4 (1998), S. 129-136 
    Details
    ISSN: 1572-9788
    Schlagwort(e): differential display ; gene expression ; hybrid vigor ; molecular marker heterozygosity ; Oryza sativa L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Using differential display analysis, we assessed the patterns of differential gene expression in hybrids relative to their parents in a diallel cross involving 8 elite rice lines. The analysis revealed several patterns of differential expression including: (1) bands present in one parent and F1 but absent in the other parent, (2) bands observed in both parents but not in the F1, (3) bands occurring in only one parent but not in the F1 or the other parent, and, (4) bands detected only in the F1 but in neither of the parents. Relationships between differential gene expression and heterosis and marker heterozygosity were evaluated using data for RFLPs, SSRs and a number of agronomic characters. The analysis showed that there was very little correlation between patterns of differential expression and the F1 means for all six agronomic traits. Differentially expressed fragments that occurred only in one parent but not in the other parent or in F1 in each of the respective crosses were positively correlated with heterosis and heterozygosity. And conversely, fragments that were detected in F1s but in neither of the respective parents were negatively correlated with heterosis and heterozygosity. The remaining patterns of differential expression were not correlated with heterosis or heterozygosity. The relationships between the patterns of differential expression and heterosis observed in this study were not consistent with expectations based on dominance or overdominance hypotheses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009685820649
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  • 86
    Digitale Medien
    Digitale Medien
    Competing Models of Stability in Complex, Evolving Systems: Kauffman vs. Simon (1998)
    Springer
    Biology and philosophy 13 (1998), S. 541-554 
    Details
    ISSN: 1572-8404
    Schlagwort(e): complex natural system ; stability ; evolvability ; decomposable hierarchy ; genetic network ; Random NK Boolean Network
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Philosophie
    Notizen: Abstract I criticize Herbert Simon's argument for the claim that complex natural systems must constitute decomposable, mereological or functional hierarchies. The argument depends on certain assumptions about the requirements for the successful evolution of complex systems, most importantly, the existence of stable, intermediate stages in evolution. Simon offers an abstract model of any process that succeeds in meeting these requirements. This model necessarily involves construction through a decomposable hierarchy, and thus suggests that any complex, natural, i.e., evolved, system is constituted by a decomposable hierarchy. I argue that Stuart Kauffman's recent models of genetic regulatory networks succeed in specifying processes that could meet Simon's requirements for evolvability without requiring construction through a decomposable hierarchy. Since Kauffman's models are at least as plausible as Simon's model, Simon's argument that complex natural systems must constitute decomposable, mereological or functional hierarchies does not succeed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006567306546
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  • 87
    Digitale Medien
    Digitale Medien
    Pharmaceutical development of a parenteral lyophilized formulation of the investigational antitumor neuropeptide antagonist [Arg6, D-Trp7,9, MePhe8]-Substance P {6-11} (1998)
    Springer
    Investigational new drugs 16 (1998), S. 99-111 
    Details
    ISSN: 1573-0646
    Schlagwort(e): Antagonist G ([Arg6, D-Trp7,9,,MePhe 8 ]-Substance P {6-11}) ; analytical characterization ; parenteral formulation ; stability ; oxidative degradation products
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Abstract The aim of this study was to develop a stable parenteral dosage form for the investigational cytotoxic drug [Arg6, D-Trp7,9, MePhe8]-Substance P {6-11} (Substance P Antagonist G; Antagonist G). Antagonist G bulk drug was structurally and analytically characterized. The drug exhibits excellent aqueous solubility, although relatively poor aqueous stability characteristics. Lyophilization was, therefore, selected as the manufacturing process. Differential scanning calorimetry studies were conducted to determine the freeze-drying cycle parameters which resulted in a stable, lyophilized formulation of Antagonist G. The prototype, containing 50 mg Antagonist G per vial, was found to be the optimal formulation in terms of solubility, length of the freeze-drying cycle, stability, and dosage requirements in the planned phase I clinical trials. Quality control of the freeze-dried formulation showed that the manufacturing process does not change the integrity of Antagonist G. Shelf life studies demonstrated that the formulation is stable for at least 3 years, when stored at 2–8°C in a dark environment. Oxidative degradation products of Antagonist G were isolated and structurally characterized by mass spectrometry, nuclear magnetic resonance spectroscopy, and infrared spectroscopy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006041024109
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  • 88
    Digitale Medien
    Digitale Medien
    The vibration and stability analysis of moderate thick plates by the method of lines (1992)
    Springer
    Applied mathematics and mechanics 13 (1992), S. 523-531 
    Details
    ISSN: 1573-2754
    Schlagwort(e): moderate thick plate ; vibration ; stability ; method of lines
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Mathematik , Physik
    Notizen: Abstract The method of lines based on Hu Hai-chang's theory for the vibration and stability of moderate thick plates is developed. The standard nonlinear ordinary differential equation (ODE) system for natural frequencies and critical load is given by use of ODE techniques, and then any indicated eigenvalue could be obtained directly from ODE solver by employing the so-called initial eigenfunction technique instead of the mode orthogonality condition. Numerical examples show that the present method is very effective and reliable.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02451515
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  • 89
    Digitale Medien
    Digitale Medien
    The auto-adjustable damping method for solving nonlinear equations (1998)
    Springer
    Applied mathematics and mechanics 19 (1998), S. 163-168 
    Details
    ISSN: 1573-2754
    Schlagwort(e): nonlinear equation ; stability ; Newton's method ; auto-adjustable damping method ; the vector of damping factors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Mathematik , Physik
    Notizen: Abstract The general approach for solving the nonlinear equations is linearizing the equations and forming various iterative procedures, then executing the numerical simulation. For the strongly nonlinear problems, the solution obtained in the iterative process is always difficult, even divergent due to the numerical instability. It can not fulfill the engineering requirements. Newton's method and its variants can not settle this problem. As a result, the application of numerical simulation for the strongly nonlinear problems is limited. An auto-adjustable damping method has been presented in this paper. This is a further improvement of Newton's method with damping factor. A set of vector of damping factor is introduced. This set of vector can be adjusted continuously during the iterative process in accordance with the judgement and adjustment. An effective convergence coefficient and quichening coefficient are employed to relax the restricted requirements for the initial values and to shorten the iterative process. Then, the numerical stability will be ensured for the solution of complicated strongly nonlinear equations. Using this method, some complicated strongly nonlinear heat transfer problems in airplanes and aeroengines have been numerically simulated successfully. It can be used for the numerical simulation of strongly nonlinear problems in engineering such as nonlinear hydrodynamics and aerodynamics, heat transfer and structural dynamic response etc.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02457684
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  • 90
    Digitale Medien
    Digitale Medien
    Disturbances to a rotating fluid with a sphere moving along the axis of rotation (1998)
    Springer
    Applied mathematics and mechanics 19 (1998), S. 861-867 
    Details
    ISSN: 1573-2754
    Schlagwort(e): rotating fluids ; motion of body ; small disturbances ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Mathematik , Physik
    Notizen: Abstract In this paper, the disturbances to a uniformly rotating ideal fluid with a sphere moving steadily along the axis of rotation are analysed by using linearization theory, the equations of disturbance, pressure and disturbance stream function governing the stability of motion are derived based on the assumption that the flow is rotational symmetric. The equation of disturbance stream function is analysed with the method of normal modes, and the constraints on wave number and wave velocity of the nontrivial neutral disturbances are established and the exact expression of the neutral disturbances are obtained. The conclusion is drawn that three are three kinds of possible forms of neutral disturbances.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02458241
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  • 91
    Digitale Medien
    Digitale Medien
    Stable production of an analog of human tissue plasminogen activator from culturedDrosophila cells (1992)
    Springer
    Cytotechnology 10 (1992), S. 157-167 
    Details
    ISSN: 1573-0778
    Schlagwort(e): Drosophila cell culture ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract We have studied the expression of an analog of human tissue plasminogen activator, FK2P, inDrosophila Schneider 2 cells. A number of promoters were tested, including theDrosophila metallothionein promoter (MTd), baculovirus immediate early promoter (IE),Drosophila copia promoter, mouse metallothionein promoter, cytomegalovirus immediate early promoter with or without intron, SV40 immediate early promoter, and human elongation factor 1α promoter. Two of these promoters drove significant expression of FK2P. The MTd promoter is tightly regulated and upon induction with copper or cadmium expression of FK2P increases as much as 180-fold, accumulating in the culture medium to about 7 μg FK2P/106 cells/day as determined by ELISA. The IE promoter can direct the constitutive expression to yield about 0.4 μg FK2P/106 cells/day. The production of FK2P in these cell lines remains at about the same level after repeated passages, even in the absence of selective pressure. The FK2P accumulated in the culture medium is fully active in an assay using a chromogenic substrate for serine proteases. Western immunoblot analysis shows that the product remains predominately as single-chain molecules in serum-free medium, while in serum-containing medium two-chain material occurs as expected due to the presence of plasmin in serum. Judged from the size in Western immunoblots, the FK2P produced is glycosylated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00570892
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  • 92
    Digitale Medien
    Digitale Medien
    Bifurcations in Spherical MHD Models (1998)
    Springer
    Studia geophysica et geodaetica 42 (1998), S. 320-327 
    Details
    ISSN: 1573-1626
    Schlagwort(e): MHD ; stability ; bifurcations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Architektur, Bauingenieurwesen, Vermessung , Geologie und Paläontologie , Physik
    Notizen: Abstract A series of numerical studies on the behaviour of magnetic fields and motions in a spherical body of an electrically conducting incompressible fluid have been carried out. The magnetic field was assumed to be maintained by a given electromotive force inside the body and to continue as a potential field in outer space. In view of the motion an external forcing was taken into account, and boundary conditions were considered which correspond to a stress-free surface. The stability of several steady states has been studied as well as the evolutions starting from unstable states. In this paper a configuration with a poloidal magnetic field and a differential rotation, both symmetric about the same axis, is considered. This configuration is stable only for sufficiently small Hartmann numbers but evolves, if disturbed, in the case of larger Hartmann numbers toward a non-axisymmetric state. In this case the well-known symmetrization effect of differential rotation in magnetic fields is destroyed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1023356720631
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  • 93
    Digitale Medien
    Digitale Medien
    The stability in neural networks with interneuronal transmission delays (1998)
    Springer
    Applied mathematics and mechanics 19 (1998), S. 457-462 
    Details
    ISSN: 1573-2754
    Schlagwort(e): neural networks ; equilibrium ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Mathematik , Physik
    Notizen: Abstract In this paper, some sufficient conditions are obtained for the global asymptotic stability of the equilibrium of neural networks with interneuronal transmission delays of the type $$x'_i (t) = - b_i x_i (t) + \sum\limits_{j = 1}^n {\omega _{\ddot y} f_j (x_j (t - \tau _j )) + p_i (t 〉 0;i = 1,2, \cdots ,n)} $$
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02457788
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  • 94
    Digitale Medien
    Digitale Medien
    Timed Petri Nets in Hybrid Systems: Stability and Supervisory Control (1998)
    Springer
    Discrete event dynamic systems 8 (1998), S. 137-173 
    Details
    ISSN: 1573-7594
    Schlagwort(e): hybrid systems ; switched systems ; timed Petri nets ; stability ; supervisory control
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract In this paper, timed Petri nets are used to model and control hybrid systems. Petri nets are used instead of finite automata primarily because of the advantages they offer in dealing with concurrency and complexity issues. A brief overview of existing results on hybrid systems that are based on Petri nets is first presented. A class of timed Petri nets named programmable timed Petri nets (PTPN) is then used to model hybrid systems. Using the PTPN, the stability and supervisory control of hybrid systems are addressed and efficient algorithms are introduced. In particular, we present sufficient conditions for the uniform ultimate boundness of hybrid systems composed of multiple linear time invariant plants which are switched between using a logical rule described by a Petri net. This paper also examines the supervisory control of a hybrid system in which the continuous state is transfered to a region of the state space in a way that respects safety specifications on the plant's discrete and continuous dynamics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008293802713
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  • 95
    Digitale Medien
    Digitale Medien
    Modified Conjugate Gradient Method for the Solution of Ax=b (1998)
    Springer
    Journal of scientific computing 13 (1998), S. 173-183 
    Details
    ISSN: 1573-7691
    Schlagwort(e): Modified conjugate gradient method ; conjugate gradient method ; Krylov space ; convergence rate ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract In this note, we examine a modified conjugate gradient procedure for solving $$A\underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{x} = \underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{b}$$ in which the approximation space is based upon the Krylov space ( $$A\underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{x} = \underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{b}$$ ) associated with $$\sqrt A$$ and $$\underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{b}$$ . We show that, given initial vectors $$\underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{b}$$ and $$\sqrt A \underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{b}$$ (possibly computed at some expense), the best fit solution in $$K^k \sqrt A ,\underset{\raise0.3em\hbox{$\smash{\scriptscriptstyle-}$}}{b}$$ can be computed using a finite-term recurrence requiring only one multiplication by A per iteration. The initial convergence rate appears, as expected, to be twice as fast as that of the standard conjugate gradient method, but stability problems cause the convergence to be degraded.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1023222110984
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  • 96
    Digitale Medien
    Digitale Medien
    Constrained Learning in Neural Networks: Application to Stable Factorization of 2-D Polynomials (1998)
    Springer
    Neural processing letters 7 (1998), S. 5-14 
    Details
    ISSN: 1573-773X
    Schlagwort(e): constrained learning ; factorization ; feedforward networks ; IIR filters ; polynomials ; stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract Adaptive artificial neural network techniques are introduced and applied to the factorization of 2-D second order polynomials. The proposed neural network is trained using a constrained learning algorithm that achieves minimization of the usual mean square error criterion along with simultaneous satisfaction of multiple equality and inequality constraints between the polynomial coefficients. Using this method, we are able to obtain good approximate solutions for non-factorable polynomials. By incorporating stability constraints into the formalism, our method can be successfully used for the realization of stable 2-D second order IIR filters in cascade form.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009655902122
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  • 97
    Digitale Medien
    Digitale Medien
    Genetic and physiological evidence for the production of N-acyl homoserine lactones by Pseudomonas syringae pv. syringae and other fluorescent plant pathogenic Pseudomonas species (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 569-582 
    Details
    ISSN: 1573-8469
    Schlagwort(e): quorum-sensing ; gene expression ; autoinducer ; secondary metabolites
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract N-acyl homoserine lactones (AHLs) function as cell density (quorum) sensing signals and regulate diverse metabolic processes in several gram negative bacteria. We report that strains of Pseudomonas syringae pvs. syringae (Pss), tabaci and tomato as well as P. corrugata and P. savastanoi produce difussible AHLs that activate the lux operons of Vibrio fischeri or the tra::lacZ fusion of Agrobacterium tumefaciens. In Pss strain B3A, AHL production occurs in cell density dependent manner. Nucleotide sequence and genetic complementation data revealed the presence of ahlIPss, a luxI homolog within the Ahl+ DNA of Pss strain B3A. The $$ahlI_{Pss}^ + $$ DNA expresses in AHL-deficient strains of P. fluorescens and E. carotovora subsp. carotovora (Ecc), and restores extracellular enzyme production and pathogenicity in the Ecc strain. The derivatives of Pss strains B3A and 301D carrying chromosomal ahlI::lacZ do not produce AHL, but like their wild type parents, produce extracellular protease and the phytotoxin syringomycin as well as elicit the hypersensitive reaction in tobacco leaves. While these strains also produce a basal level of β-galactosidase activity, the expression of ahlI::lacZ is substantially stimulated in the presence of multiple copies of the $$ahlI_{Pss}^ + $$ DNA or by the addition of cell-free spent cultures containing AHL. The activation of β-galactosidase production occurs with spent cultures of some, but not all Pseudomonas strains which produce AHL as indicated by the Lux and tra::lacZ assays. Pss strains deficient in the global regulatory genes, gacA or lemA, produce very low levels of AHL. Since inactivation of ahlIPss eliminates AHL production and since Ahl+ Pseudomonas strains carry the homolog of ahlIPss, we conclude that ahlIPss specifies a key step in AHL biosynthesis and it has been conserved in many plant pathogenic pseudomonads.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008651300599
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    Digitale Medien
    Digitale Medien
    Senescence-induced RNases in tomato (1998)
    Springer
    Plant molecular biology 36 (1998), S. 439-449 
    Details
    ISSN: 1573-5028
    Schlagwort(e): ethylene ; gene expression ; leaf senescence ; RNase ; tomato ; wounding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A main feature of leaf senescence is the hydrolysis of macromolecules by hydrolases of various types, and redistribution of released materials. We have initiated a study for the characterization of RNases involved in nucleic acid catabolism during senescence. Using a PCR-based cloning approach we isolated from tomato two senescence-induced RNase cDNA clones. Each of these cDNAs hybridized to a senescence-induced transcript in northern analysis. One RNase cDNA was identical to the tomato LX RNase while the second corresponded to the LE RNase. Both LX and LE RNase genes had originally been demonstrated to be induced after phosphate starvation of tomato cell culture but nothing was known about their expression or function in plants. We observed that the expression of the LX and LE genes is induced in leaves during an advanced stage of senescence with the LX transcript level being much more induced than that of LE. Low-level expression of the RNase genes was observed in flowers and artificially senescing detached leaves while no expression could be detected in stems, roots, or fruits at different ripening stages. Ethylene activated the LX gene expression in detached young leaves while LE gene expression, which could be transiently induced by wounding, appeared to be activated by abscisic acid. We suggest that the LX RNase has a role in RNA catabolism in the final stage of senescence, and LE may function during wounding as a plant defense protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005993024161
    Standort Signatur Erwartet Verfügbarkeit
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  • 99
    Digitale Medien
    Digitale Medien
    Molecular cloning and mRNA localization of tomato pollen profilin (1998)
    Springer
    Plant molecular biology 36 (1998), S. 699-707 
    Details
    ISSN: 1573-5028
    Schlagwort(e): actin cytoskeleton ; in situ hybridization ; gene expression ; profilin ; RT-PCR ; tomato pollen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 µm thick anther sections using a non-radioactive labeling method reveals that LePro1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro1 belongs to the former class.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005971327353
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Digitale Medien
    Digitale Medien
    Induction of AGAMOUS gene expression plays a key role in ripening of tomato sepals in vitro (1998)
    Springer
    Plant molecular biology 36 (1998), S. 733-739 
    Details
    ISSN: 1573-5028
    Schlagwort(e): AGAMOUS ; tomato ; ripening ; calyx ; gene expression ; sepals ; in vitro
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In vitro culture of VFNT Cherry tomato sepals (calyx) at 16–21 °C results in developmental changes that are similar to those that occur in fruit tissue [10]. Sepals become swollen, red, and succulent, produce ethylene, and have increased levels of polygalacturonase RNA. They also produce many flavor volatiles characteristic of ripe tomato fruit and undergo similar changes in sugar content [11]. We examined the expression of the tomato AGAMOUS gene, TAG1, in ripening, in vitro sepal cultures and other tissues from the plant and found that TAG1 RNA accumulates to higher levels than expected from data from other plants. Contrary to reports on the absence of AGAMOUS in sepals, TAG1 RNA levels in green sepals from greenhouse-grown plants is detectable, its concentration increasing with in vitro ripening to levels that were even higher than in red, ripe fruit. Sepals of fruit on transgenic tomato plants that expressed TAG1 ectopically were induced by low temperature to ripen in vivo, producing lycopene and undergoing cell wall softening as is characteristic of pericarpic tissue. We therefore propose that the induction of elevated TAG1 gene expression plays a key role in developmental changes that result in sepal ripening.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005941330004
    Standort Signatur Erwartet Verfügbarkeit
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