ISSN:
1573-5036
Keywords:
ELISA
;
inoculation
;
nodulation
;
pea
;
Pisum sativum L.
;
Rhizobium leguminosarum
;
strain-specific resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Symbiotic interactions between peas and Rhizobium leguminosarum were investigated by inoculating four pea lines, three of which are strain-specific resistant to the European strain 311d, with various combinations of two strains of Rhizobium, 311d and Tom++. The strains were almost equally good to infect the susceptible European cultivar Hero when added singly inoculated. After mixed inoculation (1:1 proportion) strain analysis by ELISA revealed that the nodules were preferentially formed by 311d, although some Tom++ nodules were also found mainly on the upper part of the root. Our conclusion is that Tom++ is less compatible in comparison with 311d. In addition, we found that as the Hero plants emerged, they were becoming more resistant towards infection with not adapted bacteria. The strain-specific resistant lines from Afghanistan belong to two different systems: Afgh. I, completely resistant to 311d and highly nodulating with Tom++, and Afgh. III, incompletely resistant to 311d and poorly nodulating with Tom++. Mixed inoculations resulted in nodule depressions, as compared to single inoculations with Tom++ ranging from 87% to 14%. The ability of 311d to block infection sites on the roots were found to depend on the degree of symbiotic adaptation between Afgh. I and Tom++, respectively Afgh. III and Tom++. Strain analysis after double strain inoculation of Afgh. I plants revealed that some nodules were induced by strain 311d. Thus, the presence of Tom++ in this case influences the degree of host resistance. However, in Afgh. III plants the resistance towards nodulation were unaffected by the presence of Tom++. We suggest that the degree of symbiotic adaptation may change the barrier of resistance towards infection.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00012317
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