ALBERT

Notes: Abstract. White rot (Sclerotium cepivorum) causes serious losses in Allium crops throughout the world. The pathogen produces sclerotia which survive for long periods and are the main source of inoculum. Sclerotial germination is stimulated by the host and new sclerotia are produced on the host near the soil surface. Allium crops are cultivated in various systems and environments and no one method of control is effective. There is increasing interest in control strategies based on combinations of treatments which decrease the populations of sclerotia in the soil, thereby improving the effectiveness of present methods of control. Materials and methods being tested for inclusion in programmes of integrated control include germination stimulants, soil fumigants, solar heating, roguing, aerobic composting, microbial control and combined chemical/microbial control with fungicide-resistant micro-organisms.

Notes: Abstract. The relationship between take-all and grain yield was investigated in a first spring wheat crop grown in a field experiment on artificially infested soil. Different incidences and severities of disease were obtained by using different cultivations to incorporate equal amounts of inoculum (killed oat grains colonized by the fungal pathogen) at different depths. The intention of incorporating inoculum at different stages in the same sequence of cultivations to achieve identical soil conditions with inoculum at different depths was not entirely successful because of weather conditions.For most sampling dates and different assessments of disease, there was a strong relationship between yield and disease: regression coefficients were negative and significant (P= 0.01). The linear regression model using logit transformations of disease data from infestations achieved using similar sequences of cultivations accounted for 〉 70% of the variance at all sampling dates, but with untransformed data (percentage plants and percentage roots infected) percentages of variance accounted for were much less at sampling times before anthesis. The plot area affected by premature ripening (whiteheads) also correlated well with yield where similar sequences of cultivations were used, but less well where rotovating to different depths created different soil conditions.The results are discussed in relation to published results from (1) farm surveys, (2) field experiments with natural infection and (3) experiments using different amounts of artificially-produced inoculum. The wider application of artificially-produced inoculum in field experiments on take-all is also considered.

Notes: Abstract. A rotation trial of four years’ pasture followed by two years’ arable was used to study the effect of cropping on the morphological and hydraulic properties of soil. An adjacent paddock in grass for the past 35 years was included as a permanent pasture reference. Initial infiltration and field saturated hydraulic conductivity (Kfs) were least for cultivated soil and increased with increasing time under pasture. This could be explained by the contrasting porosities of resin-impregnated blocks of undisturbed soil which had been infiltrated with methylene blue dye. Small Kfs values for cultivated soil resulted mainly from a thin surface crust, although pore discontinuity at the depth of the cultivation pan (130 mm) could also have contributed. Greater Kfs values under short-term pasture resulted primarily from water flowing through biogenic pores connected to the surface. The greatest Kfs values were in soil that had been under pasture for 35 years (P35). This was attributed to flow through biogenic pores and fissures associated with the strongly-developed subangular blocky structure. The amount of water that infiltrated the two- and four-year pasture soils (P2 and P4) under ponding was 2.5 and 5 times greater, respectively, than the soil that had been cultivated for two years (C2).As irrigation duration cannot be varied under the border-dyking system used on the Canterbury Plains, the interval between irrigations must be varied if the same total amount of water is to be applied to each of these soils through the season. The interval should be less for the cultivated soil than for those under pasture, and should increase with increasing time under pasture (i.e. P35 〉 P4 〉 P2 〉 C2).

Notes: Abstract. N-(n-butyl) thiophosphoric triamide (NBPT) is the most effective compound currently available for retarding hydrolysis of urea fertilizer in soil and for decreasing ammonia volatilization and nitrite e accumulation in soils treated with urea. It is a poor inhibitor of plant or microbial urease, but decomposes quite rapidly in soil with formation of N-(n-butyl) phosphoric triamide, which is a potent inhibitor of urease activity.The adverse effects of urea fertilizers on seed germination and seedling growth in soil are due to ammonia produced through hydrolysis of urea by soil urease. They can be eliminated by addition of a urease inhibitor to these fertilizers.The leaf-burn commonly observed after foliar fertilization of soybeans with urea results from accumulation of toxic amounts of urea in the soybean leaves rather than formation of toxic amounts of ammonia through urea hydrolysis by leaf urease. Leaf-burn is accordingly increased rather than decreased by addition of a urease inhibitor to the urea fertilizer applied.

Notes: Abstract. In most soils of temperate regions nitrate is not held on soil surfaces and moves freely in solution. But when soils carry positive charges, nitrate is held as an exchangeable anion. As a result, leaching of nitrate is delayed relative to the movement of water. The delay can be predicted provided the anion exchange capacity (AEC) can be measured and the concentration of counter-anions is known. For soils with variable charge, the AEC varies with both pH and ionic strength, and the effective AEC should be determined under conditions similar to those in soil solution. A simple leaching method is described which satisfies this requirement. Delays in the leaching of nitrate measured in columns of repacked soil were strongly related to the AEC.

Notes: Abstract. A brief resumé of the organisms involved in the nitrogen cycle is given. Benefits accruing to micro-organisms are considered in two categories: (1) where the reaction product is incorporated into cells (nitrogen-fixing and nitrate assimilating species), (2) where the reaction is used to provide energy for growth (nitrifying and denitrifying species). Some aspects of nitrogen cycling in soils are briefly considered, including inhibition of nitrification, the importance of C/N ratios and nitrate pollution.

Notes: Abstract. Autotrophic nitrifiers such as Nitrosomonas use ammonia mono-oxygenase for the initial stage of ammonia oxidation. Nitrification inhibitors have this enzyme as their site of action. Their mechanisms include alternative substrates, suicide substrates and cuprous copper chelators.In heterotrophs, organic nitrogen is normally in the fully reduced state, but a few cell metabolites contain N-O bonds. The synthesis and breakdown of such compounds provides a mechanism for heterotrophic nitrification. A non-enzymic mechanism for nitrogen-oxidation involves hydroxyl radicals produced by the Fenton reaction. Heterotrophic nitrification is particularly important in woodland soils, where wood-rotting fungi use free radicals to break down lignin. Tests for a radical mechanism are described.

Notes: Abstract. The workshops covered various aspects of nitrogen in the environment, with special emphasis on the problems posed by nitrogenous compounds as pollutants.

Notes: Abstract. The standard of English in papers submitted to Soil Use and Management varies from excellent to disgraceful. Good scientific English is direct and simple in structure. It uses familiar words in their correct sense and order with the minimum of qualification. Slovenly prose may imply slipshod research, and prolixity an inability to think clearly.Make your meaning plain. Express itSo we'll know, not merely guess it.(G.V. Jacks, The Summary)

Notes: Abstract. Machinery was designed specifically for relay-cropping on permanent raised beds (150 mm high and 1.5 m wide) in northern Victoria. This machinery enabled maize (Zea mays) to be successfully sown at 2, 4 and 5 weeks before harvest, and 1 day after harvest (Control), of wheat (Triticum aestivum). The sowing equipment consisted of a four-row cultivator, behind which were four precision seeders. The wheels (250 mm in diameter) were spaced at 1.5 m to track along the base of the furrows. In one pass on each bed, the sowing equipment tilled two strips (each 50 mm wide, 30 mm deep and 50 mm from the outer row of wheat) and sowed maize, with little damage to the wheat crop. We extended the axle of the trailed harvester so that the wheels (250 mm in diameter) were 3 m apart, and moved the drawbar 300 mm to one side so that all wheels ran along the base of the furrows. There were no significant differences between treatments in yield (mean 2.9 t ha-1) of dryland wheat, in final emergence percentage (mean 89%) or in early growth of irrigated maize. The maize yielded significantly less grain in the treatment sown at 5 weeks (9.6 t ha-1), but not 2 or 4 weeks (mean 10.6 t ha-1) before the wheat was harvested, than in the Control (10.8 t ha-1). The wheat and maize yielded more grain than those grown traditionally as sole crops in northern Victoria.

Notes: Abstract. Wet and droughty soils and those on steep slopes are now marginal for agriculture in Denmark. A nation-wide map of these soil types has therefore been made to show their distributions. This was based on an existing soil database containing maps and analytical data. The paper describes the methods used to extract and display the data.

Notes: Abstract The co-regionalization between relative elevation and zinc concentration was used to map zinc concentration in the soil of the Geul floodplain in the southern Netherlands by co-kriging from 154 observations. Point co-kriging and point kriging for estimating zinc content in the soil were compared in terms of kriging variances. Another 45 samples were used to compare the precision of the estimated values in terms of squared and absolute estimation errors. Point co-kriging produced better estimates of zinc concentration than either simple point kriging or linear regression from the relative elevation data alone. Moreover, the estimation variances for co-kriging are substantially smaller than those for kriging. The results suggest that knowledge of geomorphological processes can often improve the quality of interpolation maps of properties that are expensive to measure.

Notes: Abstract. Disjunctive kriging provides minimum variance estimates of properties from non-linear combinations of spatially correlated sample data. In addition it can be used to estimate the conditional probability that some critical threshold is exceeded or that there is a deficit at unsampled points. The technique has been applied to estimate and map the salinity of the soil in the Bet Shean Valley of Israel from measurements of electrical conductivity. In November 1985 the estimated electrical conductivity of the soil exceeded 4 mS per centimetre throughout most of the region, and in only a small area was the probability of salinity less than 0.2. By March 1986 the electrical conductivity had declined everywhere to less than 4 mS per centimetre, and the conditional probability of exceeding this value nowhere exceeded 0.25. Despite the fluctuation in salinity farmers seem to have it under control. The results suggest that winter wheat is likely to germinate poorly in the saltier parts of the region and that lucerne (alfalfa, Medicago sativa) is unlikely to yield its maximum over most of it. Cotton, a summer crop sown in spring, should not suffer.

Notes: Abstract. To allow land planners and managers to assess erosion under alternative management practices a model has been developed. It is based on the universal soil loss equation but uses subfactors for crop cover and management. Readily available agronomic data and field expert opinion were used in formulating the method. Locally-derived data are used to validate the model which is then applied to agricultural systems in New South Wales, ranking the relative erosion hazard associated with crop and land management practices.

Notes: Abstract. The 296 soil associations of the National Soil Map of England and Wales are placed into five categories of erosion risk. These are based on land use, landform and soil properties and take into account the extent of erosion in the uplands, and its frequency, extent and rates in the lowlands. Erosion of arable land is by water or wind, but in the uplands frost action and disturbance by sheep are also important. A large proportion of arable England (36%) is at moderate to very high risk of erosion, including much of the better drained and more easily worked land, especially sandy soils. In the uplands thin soils or deep peats are most at risk. If land use changes, because of increasing intensification of agriculture or in response to climatic changes, many soil associations will become more at risk of erosion.

Notes: Abstract. Four lithological types of chalk are recognized. Normal white chalk has 34–50% porosity, nearly all of which holds water conventionally‘available’to plants. Hard chalk and grey chalk have less porosity and available water; chalk marl has very little available water. Significant capillary rise from below the root zone can be expected in normal white chalk only, and is not halted by fragmentation. Chalk has little N and usually little P. Only grey chalk and chalk marl have much ettractable K and Mg. These also slake, impeding roots. Thus, fertilized plants tolerant of high pH can grow well on normal white chalk debris, but on raw chalk marl, e.g. Channel Tunnel spoil, they need irrigation. Interesting plants volunteer on chalk debris, even on slaked chalk marl by the sea.

Notes: Abstract. Tillage and mulching effects on the environment of the seed zone and on growth of cowpea (Vigna unguiculata) seedlings in the humid tropics were studied at Ibadan, southwestern Nigeria, in the 1987 and 1988 late cropping seasons. The split-plot design experiment had conventional tillage (ploughing and harrowing), reduced tillage (ploughing only), zero tillage and grass mulch treatments. Conventional and reduced tillage practices decreased initial bulk density and increased seedling emergence, root growth, dry matter yield and overall seedling performance. Addition of mulch increased the soil moisture in the root zone and significantly decreased maximum soil temperatures and diurnal fluctuations in temperature. This provided a more stable environment for seedling establishment and growth than the unmulched soil.

Notes: Book reviewed in this article.Heavy Metals in Soils Editor B.J. Alloway.F.A.O. Soils Bulletin 61: Radioactive fallout in soils, crops and food By F.P.W. Winteringham.Dynamics of soil organic matter in tropical ecosystems Edited by D.C. Coleman, J.M. Oades and G. Uehara.Agroforestry for soil conservation By A. Young.

Notes: Abstract. One direct measurement and two indirect estimates suggest that 35–40 kg nitrogen per hectare are deposited on arable land from the atmosphere each year in the south and east of England. This could contribute markedly to nitrate leaching and soil acidification. It may also change the flora and fauna of ‘natural’ ecosystems, as such amounts are likely to exceed the critical load.

Notes: Abstract. Four bullock-drawn tillage implements (mouldboard plough, chisel plough, sweeps, and shovels) were evaluated on a hard-setting Alfisol. Measurements included draft requirement, bulk density, cone index, soil crust strength, water content of the plough-layer and crop yield. Changes in bulk density and cone index due to tillage decreased with time and were negligible by the end of the growing season. After tillage with a mouldboard plough the crust was stronger than after tillage with other implements. The shovel cultivator enabled the soil to store more water, and required least draft per unit effective area of cut.

Notes: Abstract. During 1986 erosion on soil mulched with 6 t ha-1 of straw was compared with that on soil maintained bare by herbicides in a mature apple orchard. The orchard was on well-drained soil with a sandy loam top on a slope of 2°. Erosion was greatest in the alleys between the trees, where traffic had compacted and smoothed the soil. The mean annual soil loss on bare ground in the alleys was 0.45 t ha-1; straw mulch reduced this by 85%. Erosion was worst between July and October, when rainfall 〉 10 mm h-1 was most frequent. However, differences in soil moisture and resistance to infiltration may have also contributed to differences in erosion throughout the year.

Notes: Abstract. Biological control by manipulating agricultural practices has been used for thousands of years. In the more restricted sense, of adding antagonistic micro-organisms, biological control is still a very small part of the chemical pesticide market, but reliable products are becoming available. Achieving consistent results in realistic agricultural conditions is a problem, but there is a good possibility of controlling root diseases, especially where the soil characteristics or the environmental conditions can be controlled. The use of biological control in integrated control is successful, and in the long-term genetic engineering techniques will be important in the development of biological control. Environmental safety and effective patent protection are still being developed, but there seem to be no insuperable problems.

Notes: Abstract. The recent spread of sugar-beet rhizomania and barley yellow mosaic virus in Europe has stimulated fresh interest in plant viruses with soil-borne fungal vectors. Although there are at least 20 such viruses, most of which cause diseases of important field crops, there are many gaps in our knowledge of their epidemiology because much research has concentrated exclusively on the viruses. The vectors are lower fungi that are obligate parasites of plant roots and are therefore difficult to study experimentally. Resting spores containing virus can survive indefinitely in soils but effects of rotation have not been greatly studied. There is no quantitative information relating inoculum levels of fungus and virus to disease development. Only limited information is available about inoculum distribution in soil and factors affecting its potency. The diseases are mostly dispersed by soil movement during agricultural operations but some features of disease distribution in infested fields await adequate explanation. Little is known about interactions with other micro-organisms.

Notes: Abstract. Root diseases are often hard to identify or quantify visually, particularly by the inexperienced, so several modern methods of diagnosis from other branches of biology have been modified for soil-borne plant pathogens. Though very sensitive, most of these techniques are slower and more laborious than the routine inspection of plants for disease symptoms, yet they need less expertise and are consistently accurate. Apart from immunology, most laboratory diagnostic procedures have so far proved ill-suited for field use, as they are neither sufficiently flexible nor portable. Despite the rapid detection and identification of numerous viruses by routine enzyme-linked immunosorbent assays (ELISA), there have been until recently few suitable protocols adapted for fungi. DNA hybridization forms the basis of another group of fairly rapid diagnostic techniques which will probably be restricted to the laboratory for some while yet. Nevertheless, DNA techniques have proved valuable in various investigations, such as unravelling the phylogeny of some species of soil-borne pathogens. For the foreseeable future methods based on antibodies and DNA probes are likely to extend their domination of detection and diagnosis techniques. By making recognition easier, these techniques play complementary roles in expanding our understanding of the biology, taxonomy and ecology of soil-borne pathogens.

Notes: Abstract . There are many records of mycorrhizae, particularly ectomycorrhizae, protecting roots against soil-borne plant pathogens, though fungal or viral diseases of the green parts are usually more severe in mycorrhizal plants. There are various mechanisms by which mycorrhizae protect themselves against soil-borne diseases. The occurrence and ecological significance of these mechanisms are discussed in relation to environmental factors. Mycorrhizae will probably become a very effective means of biological control once we have learned how to manage them.

Notes: Abstract. The most important root diseases of wheat in southern Australia are take-all, rhizoctonia bare patch and cereal cyst nematode. Control of grasses in annual pastures in the year preceding wheat crops decreased take-all on wheat and the amount of the take-all fungus in soil, decreased the damage caused by Rhizoctonia, and gave yield increases. Fumigation of cereal-growing soils gave yield increases in wheat of 0.75 to 2.8 tonnes per hectare, indicating that in southern Australia soil-borne root diseases impose a major constraint on productivity. Residues of the herbicide chlorsulfuron one year after application to an alkaline soil increased root damage by Rhizoctonia in barley and decreased grain yields by 1.5 tonnes per hectare. Root damage by cereal cyst nematode was decreased by direct drilling wheat and also by having a barley cultivar resistant to the pathogen as a preceding crop. The number of cysts of cereal cyst nematode on wheat roots was increased by the application of superphosphate in bands with the seed. These results show that in southern Australia soil management strategies which decrease the levels of root disease greatly increase grain yields.

Notes: Abstract. Farm livestock typically retain 5–23% of dietary nitrogen, and consequently excrete large amounts of nitrogen, mainly in urine. Areas affected by cattle urine may receive the equivalent of several hundred kg nitrogen per hectare. Urea is usually hydrolysed to ammonium carbonate within a few days. This increases the soil pH and thereby assists volatilization of ammonia. Volatilization is also increased by soil warmth and by small soil cation exchange capacities. Over the grazing season in lowland UK about 15% of the nitrogen in urine is likely to be volatilized as ammonia, but only 1–5% of the nitrogen in dung is lost in this way.Substantial volatilization of ammonia probably occurs from animal houses and after spreading of slurry in the field. About 3–4% of fertilizer nitrogen used in the UK is lost as gaseous ammonia. Cut grass herbage also loses ammonia by volatilization, if allowed to remain in the field in wet conditions. Total annual emissions of nitrogen as ammonia from grassland and livestock in UK are probably 320 000–420 000 tonnes.

Notes: Abstract. Public perceptions of agriculture as a contributor to environmental stress are resulting in changes within agricultural production systems. Increasingly, these systems will need to be economically viable, environmentally sound, socially and politically acceptable, and conserving of natural resources-the characteristics that describe a sustainable agriculture. Improving and maintaining agricultural production systems with these characteristics must continue to be the objectives of agricultural research. Nitrogen research has contributed markedly to meeting the production and economic goals of agriculture, but it has not yet focused enough on answering socially and politically sensitive questions. Questions are posed about the environmental effects of various nitrogen management practices and the research needed to answer them is outlined. Emphasis is put on immobilization-mineralization as affected by use of nitrification inhibitors and split-application, release of nitrogen from animal manures, legumes, and other crop residues, and establishing acceptable yield goals.

Notes: Book review in this ArticleManagement Systems to Reduce Impact of Nitrates. Editor J.C. Germon

Notes: Abstract. ALES, the Automated Land Evaluation System, is a microcomputer program that allows land evaluators to build their own knowledge-based systems with which they can compute the physical and economic suitability of land map units, in accordance with the FAO's Framework for Land Evaluation. The economic suitability of a land mapping unit for a land utilization type is determined from the predicted annual gross margin per unit area. Increasing limitations result in increased costs of production, decreased yields, or both. Evaluators build decision trees to express inferences from land characteristics to land qualities, from land qualities to predicted yields, and from land qualities to overall physical suitability. A representative model is described.

Notes: Abstract. After restructuring of a heavy saline gypsum containing clay soil by intensive subsoiling to 0.7 m depth, it was possible to leach 60% of the leachable salts from the restructured soil in 28 days by inducing a lateral flow of leaching water through the soil profile.

Notes: Abstract. This paper describes experiments on the inhibitory effects of hydroquinone (HQ), phenylphos-phorodiamidate (PPDA) and N-butyl phosphorothioic triamide (NBPT) on the nitrification in soil. Incubations were carried out at 2/3 field capacity at 25°C of soil samples to which either ammonium or nitrite was added together with inhibitors. Addition of PPDA or NBPT did not influence the oxidation of ammonium. HQ. however, retarded the process significantly, and also the accumulation of nitrite. This was confirmed in experiments whereby added nitrite was followed. Some of the differences could be explained by changes in the soil pH. During incubation the evolution of the total mineral nitrogen was not importantly altered by addition of the inhibitors.

Notes: Abstract. The properties of soil under 15-year-old plantations of gmelina (Gmelina arborea) and teak (Tectona grandis) were compared with logged forest soil in south-western Nigeria. The soil was significantly denser in the 0–10 cm layer of plantation soil and total porosity less than that of forest soil. Organic carbon was significantly greater in the 0–10 cm layer of forest soil. Similarly, the concentrations of total N, exchangeable Ca, Mg and K were greater under forest soil, but the concentrations of available P were similar under all three ecosystems. The smaller organic carbon and nutrient content of plantation soil is mainly due to its more open organic matter and nutrient cycles and nutrient immobilization in the fast-growing exotics.

Notes: Abstract. The soil has been investigated at a long-term experiment examining the silvicultural and site-related effects of four tree species (Scots pine, Norway spruce, oak, common alder), planted pure or in mixtures. The experiment is located in Gisburn Forest, Yorkshire, and commenced in 1955. After 32 years, small significant differences were found for soil properties relating to soil organic matter accumulation and incorporation; the soil under the conifers and alder was slightly more acid than that under oak and grass control plots, and the conifers had thicker F and H but thinner A horizons. There was also some evidence that the conifers and alder has retarded the formation of a grey iron-deficient B horizon. Some mixed plots were differentiated from pure ones by canonical variate analysis. Soil pH has declined under all plots since 1954; the effect of mixing species on this property is discussed.

Notes: Abstract. Nutrient manipulation through fertilization or modification of the soil environment to influence nutrient availability is an important cultural control for plant disease and an integral component of production agriculture. Fertilization decreases soil-borne diseases by maximizing the inherent disease resistance of plants, by facilitating disease escape through increased nutrient availability or stimulated plant growth, and by altering the external environment to influence the survival, germination and penetration of pathogens. The flexibility in most disease-nutrient interactions permits a much broader utilization of this cultural control in decreasing disease severity than is presently practised. It is clear that the severity of most diseases can be decreased and the chemical, biological or genetic control of many plant pathogens enhanced by proper fertilization. Breeding nutrient-efficient or disease-tolerant crops and establishing cultivar requirements should further improve production efficiency.

Notes: Abstract. The aluminium (Al), iron (Fe) and Dissolved Organic Carbon (DOC) contents of the soil solution were monitored in two upland grassland and afforested podzol soils in Mid-Wales. Al organo-metallic complexes predominated in the O horizon leachates of the grassland soil, whereas inorganic monomeric Al forms dominated in the lower mineral horizons. Dissolved organic matter determines the chemistry, solubility, and transport of Al and Fe in the O horizon, and these are under strong biological control. The distributions of organic-Al, Fe and DOC within the soil profile were consistent with traditional podzolization theory. Observed increases in the molar ratios of Al:DOC in solution in the lower soil horizons may be responsible for the small solubility of Al organo-metallic complexes in those horizons. Afforestation increased the concentrations of organic-Al and Fe in the soil solution as compared with the concentrations observed for the grassland soil. Clearcutting further significantly mobilized Al and Fe from the upper soil horizon, primarily by increasing the DOC concentration in the soil water.

Notes: Abstract. Current estimates are tabulated for the quantities of nitrogen circulating in the global nitrogen cycle. Five gases, NH3, N2O, NO, NO2 and N2, dominate the movement of nitrogen between the earth's surface and the atmosphere. The input of combined nitrogen to the land surface of the earth is tentatively estimated at 290 million tonnes per year, a total which includes 74 million tonnes from fertilizers. Known outputs from land (as gaseous NH3, N2O and NOX, and as inorganic nitrogen carried to the sea by rivers) are much less, totalling 130 million tonnes per year. Emissions of N2 gas probably account for most of the difference. There has been an increase in the use of nitrogen of about 5% per year over the last ten years. The demand for fertilizer nitrogen is likely to continue to grow if the population of the world continues to increase.

Notes: Abstract. The methods available for measurement of nitrogen fixation by field grown Phaseolus vulgaris are reviewed. Phaseolus is generally considered to have little ability to fix nitrogen. This is discussed in the light of research on host genotypes and Rhizobium strains, and the influences of environment on these in isolation and in symbiosis.

Notes: Abstract. Nitrogen-fixing micro-organisms contain the metalloenzyme nitrogenase, which can be separated into two proteins with molecular weights of approximately 58000 and 220000. Molybdenum held with iron and sulphide atoms in the cofactor (FeMoco) cluster of the larger protein is probably responsible for binding and reduction of dinitrogen (N2). X-ray absorption spectroscopy indicates that the moybdenum is surrounded by three oxygen (or nitrogen) atoms, three irons and three sulphurs. Synthetic clusters with similar X-ray absorption structures to FeMoco do not, however, interact with dinitrogen. Many metal compounds, such as those with tertiary phosphines as co-ligands, can bind dinitrogen, and some containing molybdenum can also reduce it in acidic solution to produce ammonia via several intermediate compounds. These may be developed for production of ammonia fertilizer.

Notes: Abstract. 15N2 was used in a sealed controlled environment chamber to investigate the transfer of fixed nitrogen from white clover to perennial ryegrass growing in soil in pots. There was no difference in the 15N content of roots and shoots of clover plants after exposure to 15N. No labelled fixed nitrogen was detected in ryegrass plants growing with the clover plants for a period of 129 days. There was therefore no evidence of rapid direct transfer (excretion) of fixed nitrogen from clover to ryegrass.

Notes: Abstract. Microbial osmoregulation as a factor regulating the nitrogen and carbon contents of soil microbial biomass was studied in two experiments. In the first the percentages of the carbon and nitrogen occurring in the cytoplasm of Aspergillus flavus and Pseudomonas sp. were shown to be strongly influenced by osmotic stress. In the second, biomass carbon and nitrogen initially increased with increasing water stress (osmotic and matric) up to −1.0 and −1.5 MPa, respectively, but declined under greater osmotic stress. As the soil microbial carbon and nitrogen pools are affected by these stresses, allowance must be made for them when interpreting biomass measurements in water-stressed soils.

Notes: Abstract. Nematodes and protozoa assist nitrogen mineralization by consuming bacteria, and this may increase the amount of nitrogen taken up by plants so that it exceeds the amount lost by root exudation. One of the methods used to determine bacterial consumption by protozoan grazers in aquatic ecosystems, namely progressive dilution of the grazing pressure, was tested in a sandy soil. The result suggests that a basic assumption of the technique, that grazing is directly proportional to the dilution factor, is untrue for soils, possibly because the increase in moisture content with increasing dilution enhances grazer activity.

Notes: Abstract. Samples of peat were incubated with 15N-labelled ammonium sulphate, urea, wheat straw and glycine and divided into six size fractions of solid components and a water-soluble fraction. The fractions were analysed by NMR spectroscopy to study the formation of humic substances and rind how fertilizer nitrogen is immobilized in peaty soils. After six months' incubation about half of the ammonium sulphate nitrogen was still present as ammonium in the soluble fraction, the urea had been entirely metabolized to ammonium and various organic compounds, about half the straw had been decomposed to ammonium and amino acid or peptide materials, and most of the glycine had been transformed to ammonium, amide and aliphatic amine.

Notes: Effects of sowing date, fungicide seed treatments, and the head smut fungus Ustilago bullata Berk, on establishment, herbage, and seed production of prairie grass cv. Grasslands Matua (Bromus willdenowii Kunth) were measured in autumn- and spring-sown field trials in New Zealand.Autumn seedling establishment from thiram-treated seed relative to untreated seed was increased by 27% from the earliest sowing (early March), and by 65% from the latest sowing 8 weeks later. Infestation of seed with ustilospores of U. bullata reduced seedling establishment at all but the latest autumn sowing. Fungicide seed treatments had little effect on spring seedling establishment, but V. bullata infestation of seed reduced establishment compared with healthy seed, particularly after late sowings (early November).Herbage production was greater from early than from late sowings in both autumn and spring, and was less in plants grown from U. bullata infested seed than in plants from healthy seed. Enhanced autumn establishment from thiram-treated seed led to improved herbage production when plants were young, but this effect disappeared about 18 weeks after late autumn sowing. Overall herbage production from spring sowing was more than twice that from autumn sowing.A 6-week delay in autumn sowing led to a 58% reduction in seed production the following summer.Early autumn and spring sowings, together with adequate control of seedling diseases, are likely to give optimum herbage and seed production from prairie grass.

Notes: Herbage production and quality of swards of brome grass (Bromus carinatus. Hook and Am) were compared with other commonly sown grasses at two sites in Scotland. At Ayr, the comparison was with perennial ryegrass (Lolium perenne L.), cocksfoot (Dactylis glomerata L.) and timothy (Phleum pratense L.) under 6-cut (experiment 1) and 4-cut (experiment 2) regimes over 3 years with 360 kg ha−1 fertilizer N applied annually in each experiment. At Edinburgh, brome grass was compared over 3 years with perennial ryegrass and cocksfoot under a 7-cut system given 3(X)-35O kg N ha−1 year−1 (experiment 3) and with perennial ryegrass, cocksfoot and Italian ryegrass (Lolium multiflorum Lam.) under a 4-cut system given 250–325 kg N ha−1 annually (experiment 4).Over the 3 years, brome grass gave 1.18, 6.19 and 1.3% less dry matter (DM) production than the other grasses in experiments 1, 2 and 3 respectively; in experiment 4, it was 1 % less productive than Italian ryegrass but 1.2% more productive than the other grasses. The organic matter digestibility (OMD) of brome grass was lower than that of perennial ryegrass but higher than timothy at Ayr, similar to perennial and Italian ryegrasses at Edinburgh but markedly superior to cocksfoot at both sites. N concentrations in brome grass were higher than in the ryegrasses but lower than in cocksfoot. Mineral composition data showed brome grass to be high in P and K, low in Ca and Mg and very low in Na compared with corresponding concentrations in the other grasses.The variable performance of this brome grass species (B. carinatus) against commonly used grasses in the reported experiments, together with similar evidence from the literature, leads to the conclusion that it is unlikely to be suitable for widespread use in the UK; nevertheless, it has shown some promise in drought-prone situations.

Notes: Book reviews in this article: Advances in Nitrogen Cycling in Agricultural Ecosystems Edited by J.R. Wilson Ruminant nutrition. Recommended allowances & feed tables Edited by R. Jarrige Soil Husbandry Tom Batey Evaluation of straws in ruminant feeding Edited by M. Chenost and P. Reiniger

Notes: An experiment was conducted to examine the effect of concentrate supplementation on the performance of suckling lambs while grazing at variable levels of herbage allowance. Twenty-four ewes suckling twin lambs were allocated 55 d after lambing between four grazing treatments (two levels of herbage allowance × two levels of supplementation) in a 2×2 factorial design. The sward consisted of Tall Fescue and was grazed rotationally.Daily herbage organic matter (OM) allowances were 57.0 (H) and 38.5 (L) g OM kg LW−1 above 1·5 cm cutting height. Lambs were either supplemented (S) ad libitum with high-moisture whole maize grain or unsupplemented (U).Supplement intake during the 60-d grazing period was 16 kg DM and 17.5 kg DM respectively for LS and HS lambs. The effect of supplementation on lamb growth rate differed significantly between allowances: at the low allowance level, supplementation increased growth rate (287 g d−1 vs 226 g d−1), whereas it had no effect at the high level (277 g d−1 vs 276 g d−1). Growth rate of unsupplemented lambs was significantly reduced at the low level of allowance compared to the high level (226 g d−1 vs 276 g d−1). The effect of sward height on time spent grazing by unsupplemented lambs is described during the defoliation of a plot.

Notes: This study evaluated the relationships between legume proportion and dry matter (DM) yield, N yield, and herbage N concentration for three binary legume-grass mixtures In Uruguay. Two identical trials were established, one in 1983 (Trial 1) and another in 1984 (Trial 2) and were evaluated for 2 years. Treatments were white clover (Trifolium repens L.) (WC), red clover (Trifolium pratense L.) (RC), and birds-foot trefoil (Lotus corniculatus L.) (BT), each grown with tall fescue (Festuca arundinacea Schreb.) (TF) at four legume proportions, plus pure stands of TF. In Trial 1, annual DM yields of RC-TF were greater than yields of WC-TF or BT-TF and in Trial 2 RC-TF and BT-TF yielded more than WC-TF. The N concentration of either the legume or TF components usually was greater for WC-TF than other mixtures in both trials. Annual DM yields in Trial 1 were optimized by legume proportions of 47 to 59% for WC, 62 to 67% for RC-TF, and 57 to 100% for BT-TF; and N yields were optimized by 70% of WC or RC, and by 100% BT. In Trial 2, which had greater soil-N availability than Trial 1, optimum DM or N yields were achieved at lower WC proportions than in Trial 1. Changes in legume proportion did not affect legume N concentrations, but N concentration of TF in mixture was always positively and linearly related with legume proportion. It is concluded that DM yields for the first 2 years after seeding were greater for RC-TF and BT-TF than for WC-based mixtures. Herbage DM and N yields of WC-TF were optimized by lower legume proportions than for RC-TF and BT-TF. The N concentration of TF increased linearly with increasing proportions of any of the three legumes.

Notes: The numbers of lactic acid bacteria (LAB), as measured by Rogosa SL agar, on lucerne (Medicago sativa) chopped for ensiling were predicted using two methods. The first prediction method consisted of regression equations developed from two previous harvesting seasons and required inputs of wilting time between mowing and chopping, average wilting temperature and moisture content of the lucerne at chopping. The second method simulated the growth of LAB on the lucerne during wilting and needed similar inputs. The former method predicted LAB counts within one order of magnitude more than 95% of the time except for lucerne samples drier than 60% dry matter. The prediction of the simulation model had more variation than those of the regression equations, but the simulation model was applicable over a wider range of conditions. These results, together with earlier studies, provide a preliminary basis for determining when a silage inoculant containing LAB will be most beneficial.

Notes: The occurrence of Listeria spp. in pasture grass and grass silage made with various additives and preservation techniques is reported. Silage samples were collected three times (in November, February and May) from eighty dairy farms. The prevalence of Listeria spp. in pasture grass samples was 0.647 and in silage samples 0.227. Only two species, L. monocytogenes and L. innocua were observed. Listeria spp. Were isolated from the silage at least once at thirtynine farms (0.488), and L. monocytogenes at twenty-seven farms (0.338). The occurrence of Listeria was highest among the silage samples collected in November (0.288). Silages preserved with acids had the lowest prevalence of Listeria (0.194). Silages preserved in tower silos were most often free of Listeria, only 0.056 being positive. The highest prevalence of the Listeria spp. (0.324) was detected in clamp silage. The results of chemical analyses of silage statistically reflect the frequency of Listeria.

Notes: An ultrasonic rangefinder (sonic sward stick) was developed for rapidly measuring and recording the undisturbed surface height of swards. HFRO and ultrasonic sward stick measurements of undisturbed sward height over continuously grazed pasture were closely related. The measurements obtained with both instruments were related to the sward mass; the distribution of that mass between leaf, stem and dead material had little effect.

Notes: A mathematical model is presented that predicts the time-course of aerobic deterioration in grass and whole-crop maize silages. The model predicts the stability of the silage taking into account the buffering capacity of the silage, the initial contents of organic acids and ethanol, pH, the initial temperature and the initial populations of the microorganisms. The specific processes simulated include the growth of yeast and acetic acid bacteria, the oxidation of fermentation products, the consumption of oxygen and the production of carbon dioxide, the rise in temperature. and the increase in pH.The deterioration of silage is seen to be initiated by acetic acid bacteria or by yeast, or by both groups together. The factors that determine which groups will prevail are the dry matter contents and the chemical composition of the silage. The output of the model is validated by comparison of the simulated data with data from published work on the deterioration of silage.

Notes: In experiment 1, a mixture of perennial ryegrass and white clover (176 g DM kg−1) was ensiled with no addition, or with the application of Lactobacillus plantarum, 3 × 106(g fresh weight of herbage)−1 or enterobacteria, 3 × 106 (g fresh weight of herbage)−1 or both of these inoculants. Silos were incubated at either 18°C or 26°C for 7 d and sealing of half of the silos was delayed for 48 h. In experiment 2, cocksfoot (247 g DM kg−1) was ensiled with no additive, with the application of L. plantarum, 4 × 106 (g fresh weight of herbage)−1 enterobacteria, 4 × 106 (g fresh weight of herbage)−1 or ammonium nitrate, 5 g (kg herbage)−1. Silos were incubated at 18°C and sealing was delayed for 48 h.In neither experiment were the untreated silages of satisfactory quality after 35 d ensilage, having pH's of 4·3 and 6·4. and ammonia-N concentrations of 139 and 209 g N (kg total N)−1 in experiments 1 and 2 respectively. The poorer fermentation in experiment 2 reflected the lower water-soluble carbohydrate (WSC) content of the ensiled herbage (136 and 53 g WSC (kg DM)−1 in experiments 1 and 2 respectively). Inoculation with L. plantarum significantly reduced silage pH and the concentrations of ammonia-N (experiment 1 only) and ethanol and increased lactic acid concentrations. Inoculation with enterobacteria had only minor effects in each experiment.In experiment 1, incubation at 26°C increased the rate of fermentation in the silos as pH was significantly lower after 7d incubation at 26°C than at 18°C, but this effect had largely disappeared after 35 d incubation. When sealing of the silos was delayed for 48 h, the resulting silages had significantly higher pH and ammonia-N contents than silages from silos that had been sealed immediately after filling.In experiment 2, addition of ammonium nitrate significantly reduced the concentrations of ethanol and acetic acid in silages opened after 7 d. After 35 d the concentrations of ethanol were significantly reduced and those of ammonia-N increased by the addition of ammonium nitrate. There were marked increases in silage pH between 7 and 35 d ensilage and in the concentrations of ammonia-N and acetic acid in the silages that had been inoculated with ammonium nitrate at ensilage.

Notes: An examination was made of the effects of different spring treatments on the growth of white clover in a ryegrass/white clover sward. Plots were either cut once (in February, March or April) or twice (in February and April) or left uncut. Nitrogen was applied to half of the plots in each instance. The clover was sampled at intervals of approximately 3 weeks from February to June to determine numbers of leaves and growing points and weights of plant parts. Rates of leaf appearance were also observed and estimates were made of total herbage mass from ground-level cuts.Percentages of white clover in the herbage were higher in unfertilized than in fertilized plots and in defoliated than in undefoliated plots. The percentage increases that followed defoliation were usually maintained into later regrowth, showing that clover content was not automatically reduced as herbage mass increased. Increases in growing points were recorded after the beginning of April in defoliated unfertilized plots but not in undefoliated fertilized plots or in plots fertilized and defoliated twice during the spring period, in which numbers fell substantially.Inverse relationships were found between rates of leaf appearance, or the number of green leaves retained per stolon, and herbage mass, whereas heights of clover and grass leaves and the percentage of dry matter allocated to petiole rather than leaf in the clover increased with increasing herbage mass.We suggest that the observed differences between spring treatments in clover percentage result primarily from their differential effects on the formation and death of tillers and growing points in the early stages of regrowth.

Notes: Forage production based on summer annual crops is a common agricultural practice in many areas of the world where the growing season is favourable.0An investigation was conducted over 3 years to compare the dry matter (DM) yield and quality of four annual crops; maize (Zea mays L.), sunflower (Helianthus annus L.), sudangrass (Sorghum bicolor L. Moensch) and sorghum (Sorghum bicolor L. Moensch) x sudangrass; in two rainfed locations with different summer temperatures in north-west Spain (Galicia). The crops were compared at three harvest dates; first weeks of August and September and, except for sunflower, at a later silage stage.The comparative forage yields of each crop varied depending on location and harvesting date, reflecting their suitability for the climate of the location.At Mabegondo, with relatively mild summer temperatures (16.5 °C), maize throughout summer and sunflower until September produced higher DM yields than sudangrass and sorghum × sundangrass. At this location the silage DM yields for maize, sorghum × sudangrass and sundangrass were respectively 15.2, 9.92 and 7.79 t ha−1. At Puebla de Brollón, where the average temperature from June to September was about 2 °C higher than at Mabegondo, the DM production of the sudan-type crops were similar to maize, while sunflower was significantly lower at the September harvest. The silage DM yields were: maize 14.07 t ha−1 sorghum x sudangrass, 14.05 t ha−1, sudangrass 12–26 t ha−1.The in vitro dry matter digestibility (IVDMD) of maize was always higher than that of the other crops, with an average value of 700 g kg−1.It is concluded that, in north-west Spain, maize is the most suitable summer crop for maximum yield of good quality forage from the first week of August through to the normal silage stage in October.

Notes: An experiment was carried out during 1984 to study the effect of treating grass at ensiling with three commercially available inoculant-type additives (H/M Inoculant, Grass Sile and Siron), formic acid (850 g kg−1; Add-F) or no additive on grass preservation, in-silo loss, intake and animal performance. Primary growth grass ensiled from 28–29 May into concrete-walled covered silos was of high dry matter (DM, 234 g kg−1), water-soluble carbohydrate content (WSC. 212 g kg DM−1) and digestibility (MADF, 250 g kg DM−1).The untreated silage displayed good preservation and with the exception of the Sirontreated silage which showed significantly lower buffering capacity (Be) and volatile fatty acid (VFA) contents than the untreated silage, the application of inoculant-type additives did not improve silage preservation or decrease in-silo DM losses. The formic acid-treated silage displayed significantly lower Be, water-soluble carbohydrate, ash, ammonia nitrogen (g kg total N−1) and lactate contents than the untreated silage.After a 133 d storage period, silages were offered to finishing beef cattle for an 84-d period. Cattle offered the silages displayed similar and non-significant daily DM intakes, daily liveweight gains, dressing proportions and daily carcass gains. From this experiment it appears unlikely that any of the additives evaluated will improve animal performance relative to a well-preserved untreated silage.

Notes: In two field experiments acetylene (C2H2) reduction by white clover in mixed swards was compared to N2-fixed measured by 15N dilution. In both experiments, samples for C2H2 reduction were 7.5 cm diameter turves taken from plots within which microplots of 24 cm diameter were delimited and to which 15N was applied as ammonium sulphate (15NH4)2SO4). C2H2 reduction was assayed every 6–7 d. The rate of C2H2 reduction per unit length of stolon was applied to the estimated stolon length within the appropriate microplot at the time of assay, and the amount of C2H2 that would have been reduced within the microplot was estimated by integration.In experiment 1, turves taken from grass/clover swards to which 0, 1·5, 3·0, 4·5 or 6·0 g N m−2 had been applied were incubated in sealed chambers (10% C2H2, 90% air). The mean ratio of C2H2 reduced to N2 fixed during 5 weeks was 0.74:1. Application of N fertilizer lowered the proportion of assimilated N derived from N2 fixation from 95% in unfertilized swards to 83% in those receiving 6 g N m−2 (60 kg N ha−1). In experiment 2, clover roots and stolons from plots that previously had been grazed were dissected from turves and incubated in a stream of C2H2 and air (i.e. the open system). The maximum rate of ethylene (C2H4) produced during the first 12 min was taken as a measure of true nitrogenase activity. The relationship between C2H2 reduced and N2 fixed was significant (r=0.80**). The mole ratio was 0.55:1 for the 6 weeks duration of the experiment, the low ratio possibly being due to disturbance of the nodules adversely affecting acetylene reduction. Mole ratios from both experiments were well short of the theoretical 4·3:1. Using the open system does not, therefore, overcome the shortcomings of the acetylene reduction technique for measuring N2 fixation of white clover in mixed swards.

Notes: A second cut of lucerne was wilted to 500 g DM kg−1 and either left untreated (control) or treated with formic acid (4.5 1 fresh forage t−1) or with a commercial inoculum of lactic acid bacteria (105 colony forming units (cfu) g forage−1). The forages were ensiled in 2-t capacity silos for 8 months, and later fed to six lambs (mean initial weight 27.7 ±1.60 kg) in a 3x3 duplicated Latin square with 27-d periods. Portions of the untreated and additive-treated forages were also ensiled in laboratory silos at 25 ° C for intervals up to 42 d. Results from the laboratory silos showed that the major increase in ammonia-N in silage occurred between 40 h and 7 d of fermentation; during this period, both formic acid and the inoculant produced a smaller increase in ammonia-N, than did the control. The pH of inoculated silage declined from 5.74 to 4.57 in 7 d, but it took 14 d for the pH of the control silage to fall below 5.0. Formic acid treatment immediately reduced the silage pH from 5.74 to 5.10 (P 〈 0·01); the pH then remained unchanged until 21 d, after which it decreased slightly. When compared with control, lambs fed formic acid-treated silage consumed more (P 〈 0·05) digestible organic matter; the response was associated with a trend towards decreased concentration of ammonia in plasma. Inoculation of lucerne silage did not (P 〈 0·05) affect voluntary intake but increased (P〈0.05) apparent digestibility of fibre and tended to increase N retention.

Notes: The percentage of clover in a mixture of Huia white clover and Melle perennial ryegrass decreased during each of three winters. In the most severe winter, both grass and clover suffered a net loss of weight, clover losing two–thirds of its maximum weight while grass lost about one–third. In the mildest winter, grass continued to gain weight throughout, while clover lost weight. Such losses, when combined with conditions such as the use of nitrogen fertilizer, which prevent a recovery of clover percentage during summer, will lead to a long–term decrease in the clover content of a sward.The losses from the clover were contributed to by all plant parts especially leaf, which lost between 60% and 95% of lamina weight. The change from autumn to winter caused a greater decrease in the number of live leaves per shoot. and in the area and weight of individual leaves, in clover than in grass. Other work has shown that there is a greater decrease in photosynthesis in clover than in grass, probably owing to changes in canopy structure; this is one cause of clover's poor performance, though differences between the two species in the effect of winter on the distribution of the carbon fixed in photosynthesis and in losses of weight in respiration and tissue death may also be involved.

Notes: Seedlings of Rumex obtusifolius L. were grown in gaps (11 cm diameter) in an established sward at Lolium perenne L., Root competition from the surrounding sward was controlled by PVC tubes and shoot competition was controlled by cutting the sward frequently. All combinations, with and without root competition and with and without shoot competition, were used.Plants received either 40 or 80 kg N ha−1 month−1 and 0 or 300 kg K2O ha−1 and were harvested 39 d after emergence; a second harvest was made 12 d later, after shading to 20% of full sunlight.The shoot dry weight per plant of R. obtusifolius was much more affected by root competition than by shoot competition, especially at low nitrogen applications. Potassium supply had no effect.More dry matter was allocated to the leaf petioles under shaded conditions and the specific leaf area was greater. This morphological plasticity enabled R. obtusifolius to grow well under shading and to be affected only slightly by shoot competition from L. perenne.The results suggest that competition for nitrogen was the main factor limiting the initial grown of R. obtusifolius in a ryegrass sward and that the morphological characteristics of R. obtusifolius make it less susceptible to competition for light in the early stages of its development.

Notes: Five experiments were carried out in the years 1980-1983 and 1986 to study the effect of treating grass at ensiling with sulphuric acid (850 g kg−1) and formic acid (850 g kg−1) additives alone, and in mixtures with or without formalin on the preservation of grass, in vivo digestibility in sheep, in-silo loss, intake and performance of finishing cattle.Primary growth grass was ensiled in experiments 1 (3–4 June 1980), 2 (12-15 June 1981) and 3 (31 May-2 June 1982), primary regrowth grass in experiment 4 (1-2 August 1983) and secondary regrowth grass in experiment 5 (7-10 October 1986). During the ensiling period within each experiment, approximately 60 t of unwilted, double-chopped, additive-treated or untreated grass was packed into covered concrete-walled 60-t capacity silos. The dry matter (DM) contents of the ensiled grass in experiments 1, 2 and 5 ranged from 155-180 g kg−1 and were lower than those recorded in experiments 3 and 4 (214 g kg−1). With the exception of grass ensiled in experiment 2, where water-soluble carbohydrate (WSC) contents were low, at 104 g kg−1 DM, grass in all other experiments contained relatively high WSC contents ranging from 140-154 g kg−1 DM.In experiments 1, 3 and 4 all silages were well-preserved. However, in experiment 2 the 450 g kg−1 sulphuric acid-treated and formic acid-treated silages displayed significantly lower pH, buffering capacity (Bc) and ammonia nitrogen contents than the untreated silage. In experiment 5, the sulphuric acid-treated and formic acid-treated silages displayed significantly lower pH, Bc, ammonia nitrogen, butyrate and volatile fatty acid (VFA) contents than the untreated silage.Each of the silages was offered daily with various levels of a supplementary concentrate for approximately 70-d periods to twelve animals of mixed breed in experiments 1, 3, 4 and 5 and to fifteen animals in experiment 2. All animals weighed between 380-470 kg at the start of the experiments.In experiments 1, 2 and 3 there were no significant differences between silages for any of the intake or animal performance parameters. In experiment 4, cattle fed the formic acid-treated silage displayed significantly higher silage DM intakes and daily liveweight gains than those fed the sulphuric acid-treated and untreated silages; in experiment 5, cattle fed the formic acid-treated silage displayed significantly higher silage DM intakes than those fed the untreated silage. It was concluded that formic acid was a more effective silage additive than sulphuric acid.Increasing the level of supplementation significantly decreased silage DM intakes in cattle in experiments 3 and 4, and significantly increased daily liveweight gains and daily carcass in cattle in experiments 1, 3 and 4.

Notes: Rates of N accumulation were studied on sheep grazed grass swards maintained at a constant height of 5 cm for two growing seasons (1985 and 1986) and receiving no N fertilizer using a tiller tissue turnover technique. Grazing with normal excretal returns resulted in an 85-105% increase in the estimated rates of N accumulation by laminae compared with similarly grazed swards where excretal returns were prevented. Generally, increases in tiller numbers in plots receiving excreta were mainly responsible for the increased rates of N accumulation, rather than increased rates of N accumulation per tiller. Rates of N remobilization from laminae and senescence (flux of N to standing-dead litter pool) per tiller, and rates of leaf extension and leaf appearance/disappearance were unaffected by excretal returns, in urine patches increased tiller numbers and increased rates of accumulation per tiller both resulted in greater estimates of N accumulation compared with tillers not visibly affected by excreta. However, at any one time only about 11% of the plot area was affected by excreta and increases in N accumulation in excreta patches could not account for the overall plot increases in rates of N accumulation.The increased tillering in plots receiving excreta may have increased exploitation of the soil N. With an average stocking rate of 15 sheep ha−1, soil nitrate-N, but not total N (Kjeldahl) nor bulk density, was increased during the winter of the second year as a result of excretal returns.

Notes: Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10−6 MJ kg MD−1 and 160 and 191 g kg DM−1 respectively. The straw mix had an ME content of 10−1 MJ kg DM−1 and CP content of 134 g kg DM−1. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d−1) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd−1) 163, 155, 163.Animal performance was, for treatments G, Si and St respectively: milk yield (kg d−1) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg−1) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg−1) 35·3, 32·9, 33·4 (s.e.d. 0·76).

Notes: Grassland in Britain is a plagioclimax vegetation type. All lies on land originally cleared from forest and, without some form of management, would revert to forest through a process of natural succession. Traditionally managed, unsown grasslands nonetheless resemble more natural grasslands in other parts of the world and contain a substantial proportion of our native flora and fauna. They are also often important for recreational activities. Agricultural intensification over a long period has led to a loss in their extent, species and amenity. Reconciling agricultural and environmental objectives in grassland management is very difficult because increased fertility and production invariably leads to species loss. Current overcapacity in the agricultural industry is leading to the switching of some agricultural support from food production to countryside management. As a result some arable and intensively managed grass is being managed more extensively. However future production technologies may be so efficient that very many fewer stock are required with the result that much marginal grassland may revert once again to forest.

Notes: Herbage quality, yields, tiller and plant populations of ‘Grasslands Matua’ prairie grass and ‘Caramba’ tetraploid Westerwolds ryegrass were investigated in a 2-year field trial on a sandy soil. Plots were either harvested frequently (five to six cuts per year) or infrequently (four cuts). During the first year, herbage was separated into leaf, vegetative and reproductive pseudostem, and analysed separately.With very mild winters and adequate water supply, swards had good persistence and production for 2 years. Total yield in the first year (10.5 t DM ha−1) was similar for both species. Yields in the second year were (t DM ha−1) 13.4 and 18 for Matua and 11.1 and 13 for Caramba under frequent and infrequent cutting respectively. Leaf contributed 58% to yields and reproductive pseudostem 35%. Infrequently cut plots had: 23% higher dry matter yields, primarily due to higher yields of reproductive pseudostem; higher yields of most chemical components and higher contents of water-soluble carbohydrates and cell walls; lower digestibility and nitrogen content. Cell wall content was consistently higher in Matua but otherwise herbage quality was similar for the two species. It is suggested that prairie grass should be considered as a replacement for spring-sown Westerwolds ryegrass on sandy soils in The Netherlands.

Notes: White clover, (cv. Haifa) and subterranean clover (cv. Seaton Park) were grown in binary replacement series mixtures in the field at proportions of 100:0, 90:10, 50:50. 10:90 and 0:100, and cut every four weeks over three growing seasons from 1985 to 1987 at Scone, New South Wales (NSW) (32°S).Relatively low plant densities minimized competition in 1985, but in subsequent seasons Haifa and Seaton Park competed vigorously for the same resource;.; usually having relative crowding coefficients greater than 1.0, and relative yield totals greater than 1.0 in spring of 1986 and 1987, and autumn 1987. This over-yielding appeared to be due to some complementarity of leaf morphology and canopy architecture.Haifa severely depressed seed production of Seaton Park in mixtures. Haifa regenerated as an annual in 1986, but as a perennial, from surviving stolons, in 1987 owing to lower maximum temperatures and better rainfall distribution in late summer and autumn of 1987.It is concluded that white clover can survive severe competition from subterranean clover as a seedling, and if moisture allows, may severely depress seed production from subterranean clover, regardless of whether plants are annual or perennial in origin. Spatial separation may minimize competition in average years but competition effects may be severe in seasons with sufficient summer rainfall for perennation of white clover, and seasonal autumn rainfall for germination of subterranean clover.

Notes: Small swards of white clover and perennial ryegrass were established in Perlite in a heated glasshouse, as either monocultures or mixtures of equal plant numbers. On 26th March, 1984 the swards were moved outside and their growth studied over the period to 29th May. All swards received a basal level of nitrate N to simulate soil mineralization and two-thirds received additionally the equivalent of 80 kg N ha−1. Over the experimental period (26th March-29th May) clover maintained its proportion of total mixture dry weight In swards given ‘fertilizer’-N and increased its proportion in those given only ‘basal’ N, However, clover declined as a percentage of total mixture dry weight during the first period of the experiment when total leaf area index (LAI) and ambient temperatures were low, and increased its percentage later when temperature and LAI had risen. Changes in tiller and stolon growing point numbers were not good indicators of changes in dry weight. Relative yields and relative replacement rates also were not good indicators of relative performance in mixture. Where ‘fertilizer’-N was applied, clover derived less of its nitrogen from that source than grass although their uptakes per unit shoot dry weight were similar.

Notes: A field plot experiment was carried out on an established grassland sward from 1983–88 inclusive to examine the effects of time of application, chemical form of nitrogen (N) and cattle slurry dry matter (DM) content on yield and efficiency of N use. Four forms of fertilizer N (a semi-organic fertilizer, a combined 2.1:1 (w/w) semi-organic/calcium ammonium nitrate (CAN) fertilizer, CAN and urea, each supplying 300 kg N ha−1 year−1, were applied with or without unseparated or separated cattle slurry at 93 and 73 g kg−1 DM respectively, both supplying approximately 150 kg N ha−1 year−1. All fertilizers and slurries were applied in three equal dressings (February/March, May/June and July/August). The efficiency of use of fertilizer and slurry N was evaluated by measuring DM yield, N uptake and apparent recovery of N in herbage at all harvests during each growing season.Fertilizer N application significantly increased (P〈0.001) the mean yields of herbage at each harvest in all years. The form of fertilizer N had no significant effect (P〉0.05) on first harvest and total herbage yields, nor on N uptakes by herbage at the first harvest in any year. The performance of urea and of CAN was more variable at the second and third harvests relative to that of the semi-organic or combined 2.2:1 (w/w) semi-organic/CAN sources which had similar efficiencies of N use. Lower DM production was associated with reduced uptake of N. Values for mean overall apparent recovery of N ranged from 57.9 ± 2.67% for the semi-organic fertilizer to 50.2±3.05% for CAN.Unseparated cattle slurry and separated cattle slurry produced similar herbage yields and N responses that were lower and more variable than with fertilizer N. The overall mean apparent recovery of N from unseparated cattle slurry was 25.5 ± 5.03% compared to 5.0 ± 4.74% for separated cattle slurry. Efficiency of N use was highest with spring applications and least with mid-season applications. Recoveries ranged from −29% for separated cattle slurry applied in June 1984 to 56% for unseparated and separated cattle slurry applied in February 1988 and June 1987 respectively. No interactions were recorded between cattle slurry and fertilizer N in terms of DM production or N uptake by herbage.The results of this study support the use of a fertilizer N source, selected on a least-cost basis, in combination with slurry to promote spring herbage production. For subsequent production, N should be supplied in fertilizer form only. The use of urea is risky under low rainfall conditions. Mechanical separation did not improve the efficiency of use of slurry N.

Notes: A total of 1009 samples of silage made in bunker silos on commercial farms between 1972 and 1978 was analysed to investigate the effect of herbage water-soluble carbohydrate content (WSC) and weather conditions at ensilage on fermentation as measured by ammonia-N concentration and pH of first-cut grass silages.Silage dry matter (DM) content had the major effect on fermentation. Factors influencing silage DM were rainfall and hours of sunshine during silage making, and DM content of the grass cut. WSC content of herbage ensiled also had a significant effect on subsequent fermentation. The major influences on herbage WSC were hours of sunshine and rainfall during the growing season.The effect of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of silage DM.The minimum DM necessary to produce well-fermented silage without additive was approximately 260 g kg−1. Use of formic acid significantly reduced this requirement to 240 g kg−1 and to 252 g kg−1 for sulphuric add + formalin. The results indicate that the minimum herbage WSC necessary to prevent a clostridial fermentation developing in silage with a DM content of 230 g kg−1 is approximately 37 g kg−1 without additive and 30 g kg−1 with formic acid.It is concluded that on commercial farms, weather conditions i.e. amount of rainfall and sunshine prior to and at ensilage, have a greater effect on subsequent silage fermentation than additive use.

Notes: Little bluestem (Schizachyrium scoparium (Michaux) Nash), a perennial bunchgrass, is a potentially valuable species for use in restoring ground cover in shallow range sites that are subject to drought. However, there are no data on the nutritive quality of the diverse phenotypes of little bluestem found in the north central USA and south central Canada, and there are no cultivars of little bluestem that are adapted to these areas. The objectives of this study were to measure the nutritive quality of little bluestem clones sampled on different dates and to determine whether clones differed sufficiently to facilitate selection efforts to improve forage quality. Whole plant samples of little bluestem were clipped on 8 August and 8 September 1983 and 5 July and 6 August 1984 from a field nursery at Mandan, North Dakota, containing plants originating from Minnesota and North and South Dakota in the USA. Significant differences between sampling dates were found each year for in vitro digestible organic matter (IVDOM), crude protein, lignin, calcium, phosphorus (P) and potassium (K). In order to minimize the effects of maturity on chemical composition, data were statistically evaluated for clonal differences, using only the August sampling dates. Clones were further separated into north and southeast groups according to stage of maturity and latitude and moisture conditions where the clones originated. Clones were significantly different with respect to IVDOM and P in both the north and southeast groups for the August 1983–1984 data. IVDOM and P among clones averaged over years for the August sampling date ranged from 374to 521 g kg−1 and from O-9to l-6g kg−1, respectively, for the north group and from 456 to 538 g kg−1 and from 1–2 to 2–0 g kg−1, respectively, for the southeast group. The range in IVDOM and P suggests that selection to improve little bluestem quality components, especially later in the season, may be warranted. However, it is important that these results be corroborated under conditions where the effects of maturity are further diminished.

Notes: The potential productivity of perennial ryegrass/ white clover swards (GC) under continuous stocking management was assessed by comparing their performance, when grazed by sheep at sward surface heights of 3, 6 and 9 cm, with that of an all–grass sward (G) maintained at 6 cm and fertilized with 420 kg N ha–1 The grass/clover swards received no nitrogen fertilizer. The different grazing treatments had a marked effect on animal performance. In the first year for example, for treatments GC3, GC6, GC9 and G6–420 respectively, mean stocking rates to weaning were 19–7, 14–3, 8–9 and 18–4 ewes ha–1 (plus twin lambs); lamb growth rates were 223, 268, 295 and 260 g d–1and so total lamb live weight gain was 1054, 920, 630 and 1148 kg h a–1. The relative performance of the treatments was similar in all three years. All three grazing treatments had a similar effect on the composition of the grass/clover swards. Clover content increased in 1985, and was sustained in 1986 and 1987 during the main grazing season, although a marked decline in clover content during the winter led to a progressive long–term decline in both the proportion and the amount of clover.It is suggested that a management based on maintaining a sward surface height close to 6 cm (as in all–grass swards) leads to optimum performance in grass/white clover swards grazed using continuous stocking with sheep. Despite the presence of a small and declining clover content, the output of the mixed grass/clover sward managed in this way was 80%, 80% and 82% of that of a grass sward supplied with 420 kg N ha–1 in 1985, 1986, and 1987 respectively and, similarly, 83% of the output in 1987 of a grass sward receiving 210 kg N ha–1.

Notes: Intensive grazing of pastures may cause drastic and rapid changes in swards which have major effects on ingestive behaviour and diet. Twelve adult Angus cows (Bos Taurus), mean live weight of 482±19 kg, were allowed to graze on swards of lucerne (Medicago sativa L.) that were not grazed (TO), or had been grazed previously for 1 h (T1), or 2 h (T2) in a balanced changeover design. Herbage dry ma er (DM) masses (〉5 cm) were 2611, 1895 and 1441 kg ha−1; leaf fractions were 0-48, 0-29 and 0-14; and herbage DM allowances per animal were 10·6, 7·9 and 6·0 kg h−1 for TO, T1 and T2, respectively. During a 1 h measured grazing session that followed an overnight fast, cows ingested 2-93, 1·71 and 0·66 kg DM h−1 with herbage DM intakes per bite of 1·6, 0·9 and 0·4 g for T0, T1. and T2, respectively. Rates of biting did not respond to sward treatment and averaged 30 bites min−1. Intake of leaf DM was estimated at 98, 70 and 6% of total DM intake for the same treatment sequence. Utilization of herbage allowance was 0·29, 0·23 and 0·12, for TO, T1 and T2, respectively. Metabolzable energy (ME) intake per animal was 30, 17 and 5 MJ h−1 and ME intake per bite was 16, 9 and 3 KJ for TO, T1 and T2, respectively. Data show that grazing-induced differences in sward characteristics moderate both ingestive behaviour and diet.

Notes: Harvesting techniques, which involve simultaneously preserving and upgrading mature crops of whole cereals using an alkali, have been developed. When treated crops have been fed to sheep and cattle, satisfactory intakes and growth rates have been observed. With the possibility of falling cereal prices, such an end-use for grain crops may be attractive in the future. Accordingly, a mathematical model of the production, harvesting and utilization of whole-crop wheat and grass silage has been developed to examine the economic implications. Using the model, the comparative costs of growing and feeding alkali-treated whole-crop wheat in place of grass silage for winter milk production have been examined.A milk production system based on whole-crop wheat rather than grass silage is estimated to improve profits by up to £130 per cow. However, the improvement in profitability is very sensitive to assumptions about the uses for surplus land or silage arising from such a switch. Furthermore, for physiological reasons it may be prudent to restrict the quantity of whole-crop wheat fed to about 50% of the total forage intake. Thus, whole-crop cereals may best be seen as a complement to rather than a substitute for grass silage. However, in the absence of data on feeding dairy cows with treated wheat silage, research is required before the computer simulations can be corroborated.

Notes: Respiration losses, effect of sugar content, formic acid addition and inoculation were evaluated in relation to initial fermentation and silage quality in three experiments. Changes in the content of fermentable substrate during post–harvest respiration and initial fermentation were studied in the laboratory. Soluble carbohydrates were estimated as glucose, fructose, sucrose, fructans and starch by an enzymatic method.About 70% of the sugars remained after 30 h of conventional wilting, as opposed to less than 50% in a chopped sample kept in the dark. During the first days of fermentation the metabolic activity was very intensive. Sugars, which accounted for 10% of the dry matter, were fermented within 4 d. There were indications, mainly in clover silages, that substances other than water-soluble carbohydrates were used as energy sources.Low-sugar crops were obtained by shading the fields. After 3 d the sugar in the grass had decreased to half of the original content. This effect was less pronounced in clover. The amount of available substrate and dry matter (DM) content had a major influence on silage quality. In an unwilted crop at least 25 g of water-soluble carbohydrate was needed per kg of fresh material to obtain an acceptable silage quality in the controls, while 20 g was sufficient in inoculated silage. Formic acid addition makes the fermentation less sensitive to water–soluble carbohydrate content. Inoculation of willed silage increased lactic acid production and decreased the pH. There was also less variation in silage quality.

Notes: A total of eighty-nine big-bale (BB) silages made on commercial farms in South Wales during the autumn in 1983-87 were analysed to discern the effect of dry matter (DM) content on fermentation. Silages were made predominantly in late September, during fine weather from permanent pasture or perennial ryegrass pastures, which had not been grazed for 9 weeks. On average 7·8 ha of pasture were cut; most farmers attempted to pre-wilt grass for an average of 36 h prior to ensilage in individual plastic bags. Silage making was usually completed within 4 d of starting to cut. Silage ammonia-N content was inversely correlated with DM content. The results indicated that a DM content of about 280 g kg−1 was needed to produce well-preserved silage with an ammonia-N content of 100 g (kg total N)−1. Silages were, therefore, generally poorly preserved because the DM increase from wilting was only 2·3 g kg−1 h−1.

Notes: Red fescue (Festuca rubra L.) cvs Reptans, Dawson and Ruby, perennial ryegrass (Lolium perenne L.) cvs Perma and Sprinter, crested dogstail (Cynosurus cristatus L.) cv. Southlands, smooth meadow-grass (Poa pratensis L.) cvs Parade and Arena, Yorkshire fog (Holcus lanatus L.) commercial types I and II, creeping bent (Agrostis stolonifera L.) cv. Penncross and rough meadow-grass (Poa trivialis L.) cvs Omega Øtofte, Dasas and Sabre were each sown with white clover (Trifolium repens L.) cv. Donna and the productivity of the fourteen mixtures assessed under simulated grazing (six harvests per year) for 3 years. A single fertilizer-N rate (50 kg ha−1) in early spring was applied to all treatments each year.Over the 3 years, mean annual dry matter (DM) production of the swards ranged from 5·25 t ha−1 (creeping bent) to 8·71 t ha−1 (red fescue cv. Reptans), with the species ranking order of: red fescue 〉 perennial ryegrass 〉 crested dogstail 〉 smooth meadow-grass 〉 Yorkshire fog 〉 creeping bent. Rough meadow-grass only persisted over the first harvest year. Mean annual organic matter digestibility (OMD) values of the swards ranged from 0·712 (creeping bent) to 0·782 (ryegrass cv. Sprinter). Mixtures with the lowest proportion of grass (crested dogstail and smooth meadow-grass) generally gave the highest proportions of white clover. The creeping bent mixture had exceptionally low production of both components.Red fescue, crested dogstail and smooth meadow-grass showed compatibility with clover and, therefore, have potential for use in grass/clover swards. Yorkshire fog had lower compatability with clover. Some breeding effort to improve selected characteristics of promising species, e.g. improvement of low OMD in red fescue, seems warranted.

Notes: The influence of N in slurry or in mineral fertilizers on herbage yield and nitrogen fixation by white clover grown in mixed swards was investigated. Two levels of N in cattle slurry were compared with a range of mineral N fertilization. The percentage of N derived from symbiosis (% Nsym) was measured by the 15N-isotope dilution method. The measurements were made in spring 1987 in two 4-year-old field trials, and included two out of five harvests.The % Nsym was approximately 90% without N fertilization. It decreased in response to both mineral N and N in slurry but did not fall below 63%. The sum of two harvests revealed that 51·2 kg N ha−1 were fixed in the absence of N fertilization. With 75 kg mineral N ha−1 or 50 m3 cattle slurry ha−1, the yield of fixed N decreased to 17·2 and 24·9 kg ha−1, respectively. When compared on the basis of the fertilizer effect on dry matter yield and N concentration of perennial ryegrass, the decrease in yield of N fixed due to treatment with cattle slurry was less than that due to mineral N fertilizer. This was owing to the smaller extent of the depression in the proportion of white clover in the sward when the same amount of N was applied in cattle slurry, as compared with mineral fertilizer, although % Nsym responded similarly to both types of N fertilization.

Notes: Land Use and the European Environment Edited by M. Whitby and J. Ollerenshaw

Notes: A small-plot cutting trial was carried out 1983-85 at North Wyke Research Station, Devon to compare the dry matter (DM) production and response to nitrogen (N) of a naturally occurring Agrostis stolonifera-dominant sward, developed under low fertility conditions on a poorly structured soil. and a sward of Lotium perenne cv. Melle, direct-drilled in 1982 at the same site. All plots received standard inputs of P. and graded inputs of K, related to the four annual N treatments (0, 200, 400 and 800 kg ha−1, in seven equal applications). All plots were cut six times each year, except in 1983; DM yield, herbage digestibility, herbage N concentration, response to applied N, apparent recovery of applied N. and botanical composition of the swards were recorded.On average, A. stolonifera gave 69% of the DM yield of L. perenne. A. stolonifera did not out-yield L. perenne at the low N levels (0 or 200 kg N ha−1 a−1), or in mid-season. and yielded significantly (P〈0-001) less in drought conditions in 1984 (4-4 t DM ha−1 and 6-9 t DM ha−1, s.e.d.±0-22). Average digestibility of A. stolonifera was 3 units of D-value lower (P〈0-001) than L. perenne, but N concentrations were higher (3-01% and 2-53%, s.e.d. ±0-074). However, total yields of N from A. stolonifera were lower. Responses in DM production per kg N applied were lower in A. Stolonifera than in L. perenne for all N increments, except the highest, (400-800 kg N ha−1). Fitted response curves allowed interpolation of the values of DM yield (Y10) and N input (N10) where the response was 10 kg DM (kg N)−1. Values of Y10 for A. stolonifera and L. perenne averaged 7-1 t DM ha−1 and 12-9 t DM ha−1respectively. and values of Mo averaged 460 kg N ha−1 and 570 kg N ha−1, which further indicated the lower responsiveness of A. stolonifera to applied N. Apparent recovery of applied N was generally low, with significantly higher values (P〈0-001) for L. perenne in 1983 and 1984, but not in 1985. The botanical composition of L. perenne swards with no applied N showed a steady and rapid decline in ground cover by the sown species. AH L. perenne swards showed an abrupt decrease of L. perenne cover in late 1985, with invasion by A. stolonifera at all but the highest N level (800 kg N ha−1). A. stolonifera treatments receiving 400 or 800 kg N ha−1 showed initial expansion of their native L. perenne populations; however, these declined after the drought in 1984. Only low levels of L. perenne cover remained in the A. stolonifera swards at the end of the trial; these were unaffected by N level. It was concluded that under the conditions of this trial, A. stolonifera had lower DM productivity and efficiency of N use than L. perenne.

Notes: Populations of Aberystwyth S23 and S24 ryegrass tillers were individually labelled and harvested at intervals to follow their life histories and productivity in response to the effect of infection with crown rust at two levels of nitrogen fertilization.The number of tillers in a population was reduced as a result of infection with crown rust. Increased rates of tiller death due to the pathogen occurred throughout the population age-range and were most pronounced in the youngest tillers. Population changes were evident following infection and continued to be apparent in the following spring. The disease caused alterations in sward age structure, leading both to a higher proportion of older tillers in the more rust-damaged treatments and to a decrease in the weight of the herbage produced. It is concluded that rust infection of a sward may lead to changes in population structure with long-term yield effects, in addition to the immediate direct yield reductions commonly reported. The significance of these results to the ecological and agronomic balance of grassland is discussed.

Notes: The productivity of permanent swards of mixed species composition was compared with that of sown Lolium perenne, cv. Melle at five fertilizer-N rates (0, 150, 300, 450 and 900 kg N ha−1) and with L. perenne/Trifolium repens, cv. Grasslands Huia at 0 kg N ha−1. The investigation was conducted under two cutting frequencies at sixteen sites in England and Wales, representing a range of grassland environments.Annual total herbage dry matter (DM) production from both permanent and reseeded swards increased with successive increments of fertilizer-N up to 450 kg N ha−1. Herbage DM production from reseeded swards in the first year after sowing was signficantly higher than from the permanent swards, at all fertilizer-N treatments. In subsequent years the production advantage of the L. perenne reseeds was maintained only at the higher N rates, though sown L. perenne/T. repens was the most productive sward type at 0 N. Average differences in modified acid-detergent fibre suggested small advantages in herbage quality to the reseeded swards.It is concluded that, while reseeded swards are more productive in the year after sowing, many permanent swards are capable of high levels of production and that reseeding to a L. perenne sward cannot always be justified, particularly for grassland receiving low or moderate inputs of fertilizer-N.

Notes: The drying rates of grasses and lucerne, following treatment with 1% formic acid, were compared with those of untreated crops. Material was fully exposed to a flow of air in a ventilated laboratory oven set at 23 °C. Treatment with formic acid initially enhanced the rate of drying of grass, but this was followed by retardation. This reduction in the rate of drying was caused by the rapid desiccation of laminae, which reduced their effectiveness in providing a pathway for water loss from the stem. The rate of water loss directly from the stem surface was not increased by formic acid treatment, probably because the enveloping leaf sheaths acted as a protective layer. In contrast, formic acid increased drying rate in lucerne. whose stems have no such protective layer. The results suggest that chemical desiccants. which have a contact action are unlikely to have any application in making hay from grass.

Notes: In a pot experiment the pH of a hill peaty soil was adjusted to cover the range 3.8 to 6.8 with Ca(OH)2. Yield and N content of the harvested plant material was greatest at pH 6.8 in pots sown with only Lolium perenne, and greatest at soil pH 6.1 in pots sown with Trifolium repens and L. perenne. T. repens established only at soil pH between 4·3 and 6·1. A small reduction in sward yield and a large and highly significant decrease in total N recovered by the harvested material, which occurred with increase in soil pH from 3·8 to 4·3, was attributed to a combination of increased N immobilization in the soil microbial biomass and to the poor performance of both sown and self-propagating indigenous species around pH 4·3.

Notes: SPOT high-resolution visible (HRV) multispectral data were used to estimate the biomass of an improved pasture near Clare in South Australia. The study examined the effects of trees, senescent vegetation, surface wetness, herbage composition and management practices including the age of the pasture and stocking rate, on reflectance. This was undertaken by a comparison of spectral responses and through the use of partial correlation analyses. Changes in biomass had the greatest influence on reflectance, though the presence of trees and senescent vegetation influenced reflectance significantly in all wavebands. These areas were excluded from the model developed to estimate pasture biomass. The regression model was inverted and applied to a normalized difference SPOT HRV image. The accuracy of the biomass estimation was 43–81% at the 95% confidence level, or ±57.0 gm−2 of dry grass. This technique provides resource managers with a tool for routinely assessing pasture condition over large areas.

Notes: The use of brine in plastic pipes inserted into the ground is now an established technique for sampling leatherjackets in grassland. Three sequential sampling plans were compared with a standard twelve-pipe sample for the probability of a recommendation to spray and the number of sample units necessary before a decision over a range of population densities. Data derived from field studies showed a good fit with the expected outcomes. All four plan models were applied to leatherjacket distribution data from Northern Ireland, This shows the number of samples required before a decision was reduced with sequential sampling and that the use of single sequential samples would increase the risk of error. The results are discussed in the context of the physical limitations of the brine sampling procedure and it is concluded that a sequential scheme based upon groups of four pipes would reduce average sampling time by 36% without increasing error.

Notes: Small plots of a Festuca-Agrostis upland sward on a peaty gley podsol were strip-seeded during late June 1986 with white clover cvs Aberystwyth S184 or Menna at 4 kg ha−1 and defoliated early (20 August) or late (3 September) and then frequently or infrequently (every 2 weeks or 4 weeks) until the end of September. All plots were defoliated in early November, at 3-weekly intervals during the growing season in 1987 and then grazed rotationally during 1988.Satisfactory seedling establishment, representing 46% emergence, was achieved 5 weeks after sowing. The differential defoliation regimes had no persistent significant effects on clover development. S184 soon produced more leaves per seedling than Menna and a smaller proportion of its leaf number and weight were removed at each defoliation. Following large losses of leaves over the 1986–87 winter, SI84 had significantly more leaves per stolon than Menna; subsequently it also colonized the sward at a quicker rate. During 1987 amounts of herbage harvested (6.1 t ha−1) were similar with the two clover cultivars, with S184 contributing 47% and Menna 44% of this respectively. SI84 made a larger contribution to yield during May and June but Menna was more productive during September and October. During 1988 clover populations were maintained with rotational grazing without additional fertilizer inputs.The results show that, despite initial soil and climatic contraints, both small and medium-leaved clovers can be strip-seeded into upland swards with large subsequent benefits to yield and herbage quality. However, they also indicate the need for further experiments to determine the influence of sward morphology and defoliation regime on stolon branching rates and accumulation of growing points which, in turn, govern sward colonization.

Notes: Permanently marked tillers of five perennial grasses, native to the mixed-grass prairie of North America, were monitored to determine patterns of defoliation, architectural attributes influencing probabilities of defoliation. and post-defoliation responses. Frequency of tiller defoliation was greatest for one of the dominant midgrasses, intermediate for the remaining midgrass and two dominant shortgrasses. and least for the subdominant midgrass. Midgrass species, including the infrequently grazed subordinate species, were consistently grazed more intensively than the shortgrass species. However, the relative intensity of defoliation did not vary among species or grazing periods indicating that intensity of defoliation was primarily a function of pre-defoliation tiller height. Tiller architecture, including height, lamina number and the presence of reproductive culms, did not significantly influence frequency or intensity of defoliation within a species. The lack of evidence supporting tiller architecture as a selection criterion within a species suggests that animals were selecting on vegetation parameters at higher levels of vegetation organization than individual tillers. Grazed tillers of the three midgrasses exhibited greater relative rates of tiller elongation between grazing periods than non-grazed tillers, particularly early in the season when environmental conditions for growth were most favourable. The patterns of interspecific tiller defoliation observed in this study parallel the long-term patterns of grazing-induced species replacement observed in this grassland.